20.109(S13):Context-setting and primer design (Day1): Difference between revisions
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Requires a highly conserved gene. Something essential and little tolerance of mutation. Hence rRNA. | Requires a highly conserved gene. Something essential and little tolerance of mutation. Hence rRNA. | ||
(Clarridge review) | |||
*background about small subunit rRNA | *background about small subunit rRNA | ||
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Not a time of full genome sequence information | Not a time of full genome sequence information | ||
(Broad initiative) | |||
Cites for bird infections and zoonotic potential | Cites for bird infections and zoonotic potential | ||
Revision as of 12:39, 13 December 2012
Introduction
- overall motivation
Identifying pathogens in humans can suggest appropriate course of treatment. Identifying pathogens in other species can be both intellectually interesting and have applications for human health
Molecular identification of pathogens as powerful and perhaps less subjective than the identification according to morphology and phenotype
Requires a highly conserved gene. Something essential and little tolerance of mutation. Hence rRNA.
(Clarridge review)
- background about small subunit rRNA
- bacteria-specific background
- microsporidia-specific background
Over 1000 species, with 14 infecting humans
Not a time of full genome sequence information
(Broad initiative)
Cites for bird infections and zoonotic potential
Parallels to avian flu
- primer design basic background here or directly in protocols?
Protocols
Part 1: Lab practical
You and your partner may work together on the lab practical. (Note: this will not be the case for future quizzes.) You are of course welcome to give different answers should you disagree.
Part 2: Explore existing microsporidia-specific primers
Part 3: Design novel microsporidia-specific primers
For next time
Reagent list
write something here or not accessible to edit
