20.109(S10): TA notes for module 1: Difference between revisions
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'''Materials required:''' | '''Materials required:''' | ||
* | *DNA plasmid for aptamers 6-5 and 8-12. | ||
*Forward and Reverse primers | |||
*PCR Mastermix (premade or appropriate components) | |||
'''Day of Lab (T/W):''' | '''Day of Lab (T/W):''' | ||
*Prepare . | *Prepare PCR Mastermix with T7 polymerase and pyrophosphatase enzymes fresh. Keep on ice. | ||
'''Days after Lab if relevant:''' | '''Days after Lab if relevant:''' | ||
Revision as of 13:37, 20 January 2010
General notes
Key preparation:
- blah
Scheme: each pair of students will prepare aptamer-encoding DNA, then each one (?) will do an IVT, and each will do her/his own column selection. Partners will do the same ratio of 6-5:8-12 and vary column washes, or perhaps vice-versa. (Maybe latter makes more sense for balanced use of materials?)
Day 1: Amplify aptamer-encoding DNA
Materials required:
- DNA plasmid for aptamers 6-5 and 8-12.
- Forward and Reverse primers
- PCR Mastermix (premade or appropriate components)
Day of Lab (T/W):
- Prepare PCR Mastermix with T7 polymerase and pyrophosphatase enzymes fresh. Keep on ice.
Days after Lab if relevant:
How it went:
Day 2: Purify aptamer-encoding DNA
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Day 3: Prepare RNA by IVT
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Day 4: Purify RNA and run affinity column
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Day 5: RNA to DNA by RT-PCR
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Day 6: Post-selection IVT and journal club
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Day 7: Aptamer binding assay
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Day 8: Journal club
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
