20.109(S10): TA notes for module 1: Difference between revisions
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[[20.109(S10):RNA to DNA by RT-PCR (Day5)| Day 5: RNA to DNA by RT-PCR]]<br> | |||
[[20.109(S10):Post-selection IVT and journal club (Day6)| Day 6: Post-selection IVT and journal club]]<br> | |||
[[20.109(S10):Aptamer binding assay (Day7)| Day 7: Aptamer binding assay]]<br> | |||
[[20.109(S10):Journal club (Day8)| Day 8: Journal club]]<br> | |||
==General notes== | ==General notes== | ||
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Scheme: each pair of students will prepare aptamer-encoding DNA, then each one (?) will do an IVT, and each will do her/his own column selection. Partners will do the same ratio of 6-5:8-12 and vary column washes, or perhaps vice-versa. (Maybe latter makes more sense for balanced use of materials?) | Scheme: each pair of students will prepare aptamer-encoding DNA, then each one (?) will do an IVT, and each will do her/his own column selection. Partners will do the same ratio of 6-5:8-12 and vary column washes, or perhaps vice-versa. (Maybe latter makes more sense for balanced use of materials?) | ||
===[[20.109(S10):Amplify aptamer-encoding DNA (Day1)| | ===[[20.109(S10):Amplify aptamer-encoding DNA (Day1)| Day 1: Amplify aptamer-encoding DNA]]=== | ||
'''Materials required:''' | |||
*blah | |||
'''Day of Lab (T/W):''' | |||
*Prepare ... | |||
'''Days after Lab if relevant:''' | |||
'''How it went:''' | |||
===[[20.109(S10):Purify aptamer-encoding DNA (Day2)| Day 2: Purify aptamer-encoding DNA]]=== | |||
'''Materials required:''' | |||
*blah | |||
'''Day of Lab (T/W):''' | |||
*Quiz (prepared by TA) | |||
*Prepare ... | |||
'''Days after Lab if relevant:''' | |||
'''How it went:''' | |||
===[[20.109(S10):Prepare RNA by IVT (Day3)| Day 3: Prepare RNA by IVT]]=== | |||
'''Materials required:''' | |||
*blah | |||
'''Day of Lab (T/W):''' | |||
*Quiz (prepared by TA) | |||
*Prepare ... | |||
'''Days after Lab if relevant:''' | |||
'''How it went:''' | |||
===[[20.109(S10):Purify RNA and run affinity column (Day4)| Day 4: Purify RNA and run affinity column]]=== | |||
'''Materials required:''' | '''Materials required:''' | ||
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'''Day of Lab (T/W):''' | '''Day of Lab (T/W):''' | ||
*Quiz (prepared by TA) | |||
*Prepare ... | *Prepare ... | ||
Revision as of 13:12, 20 January 2010
Day 5: RNA to DNA by RT-PCR
Day 6: Post-selection IVT and journal club
Day 7: Aptamer binding assay
Day 8: Journal club
General notes
Key preparation:
- blah
Scheme: each pair of students will prepare aptamer-encoding DNA, then each one (?) will do an IVT, and each will do her/his own column selection. Partners will do the same ratio of 6-5:8-12 and vary column washes, or perhaps vice-versa. (Maybe latter makes more sense for balanced use of materials?)
Day 1: Amplify aptamer-encoding DNA
Materials required:
- blah
Day of Lab (T/W):
- Prepare ...
Days after Lab if relevant:
How it went:
Day 2: Purify aptamer-encoding DNA
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Day 3: Prepare RNA by IVT
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Day 4: Purify RNA and run affinity column
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went:
Link to Day 2 here
Materials required:
- blah
Day of Lab (T/W):
- Quiz (prepared by TA)
- Prepare ...
Days after Lab if relevant:
How it went: