20.109(S10): Spring 2010 schedule

MODULE	DAY	DATE	LECTURER	TA	LAB	note
		Tue Feb 2	ANS		Orientation	* Introductory Lecture (PDF) * Intro Pre-Lab, T/R
1	1	Thur Feb 4	JN	CB	Amplify aptamer-encoding DNA	* M1D1 Lecture * M1D1 Pre-Lab
1	2	Tue Feb 9	JN	CB	Purify aptamer-encoding DNA	* M1D2 Lecture *[[Media:S10_M1D2-ANS.pdf | M1D2 Pre-Lab]
1	3	Thur Feb 11	JN	CB	Prepare RNA by IVT
		President's Day Feb 15				No lab Feb 16 or Feb 17
1	4	Thur Feb 18	JN	CB	Purify RNA and run affinity column
1	5	Tue Feb 23	JN	CB	RNA to DNA by RT-PCR
1	6	Thur Feb 25	JN	CB	Post-selection IVT and journal club
1	7	Tue Mar 2	JN	CB	Aptamer binding assay
1	8	Thur Mar 4	JN	CB	Journal club
2	1	Tue Mar 9	AJ	XY	Start-up protein engineering	Module 1 Draft Due
2	2	Thur Mar 11	AJ	XY	Site-directed mutagenesis
2	3	Tue Mar 16	AJ	XY	Bacterial amplification of DNA
2	4	Thur Mar 18	AJ	XY	Prepare expression system
		Spring Break Mar 22-26				No lab all week
2	5	Tue Mar 30	AJ	XY	Induce protein and evaluate DNA
2	6	Thur Apr 1	AJ	XY	Characterize protein expression	Module 1 Final Report Due
2	7	Tue Apr 6	AJ	XY	Assay protein behavior
2	8	Thur Apr 8	AJ	XY	Data analysis
3	1	Tues Apr 13	ANS	NC	Start-up biomaterials engineering	Module 2 Draft Due
3	2	Thur Apr 15	ANS	NC	Initiate cell culture
		Patriot's Day April 19-20				No lab Apr 20 or Apr 21
3	3	Thur Apr 22	ANS	NC	Testing cell viability
3	4	Tue Apr 27	ANS	NC	Preparing cells for analysis
3	5	Thur Apr 29	ANS	NC	Transcript-level analysis
3	6	Tue May 4	ANS	NC	Protein-level analysis	Module 2 Final Report Due
3	7	Thur May 6	ANS	NC	Wrap-up analysis	Module 3 Report Due
3	8	Tue May 11	ANS	NC	Student presentations
		Thur May 13			No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD