20.109(S10): Spring 2010 schedule: Difference between revisions
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| [http://web.mit.edu/be/people/jasanoff.htm AJ] | | [http://web.mit.edu/be/people/jasanoff.htm AJ] | ||
| [[User:Xiaosai Yao| XY]] | | [[User:Xiaosai Yao| XY]] | ||
| | | [[20.109(S10):Start-up protein engineering (Day1)| Start-up protein engineering]] | ||
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| [http://web.mit.edu/be/people/jasanoff.htm AJ] | | [http://web.mit.edu/be/people/jasanoff.htm AJ] | ||
| [[User:Xiaosai Yao| XY]] | | [[User:Xiaosai Yao| XY]] | ||
| | | [[20.109(S10):Site-directed mutagenesis (Day2)| Site-directed mutagenesis]] | ||
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| [[User:Xiaosai Yao| XY]] | | [[User:Xiaosai Yao| XY]] | ||
| | | [[20.109(S10):Bacterial amplification of DNA (Day3)| Bacterial amplification of DNA]] | ||
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| [[User:Xiaosai Yao| XY]] | | [[User:Xiaosai Yao| XY]] | ||
| | | [[20.109(S10):Prepare expression system (Day4)| Prepare expression system]] | ||
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| [[User:Xiaosai Yao| XY]] | | [[User:Xiaosai Yao| XY]] | ||
| | | [[20.109(S10):Induce protein and evaluate DNA (Day5)| Induce protein and evaluate DNA]] | ||
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| [http://web.mit.edu/be/people/jasanoff.htm AJ] | | [http://web.mit.edu/be/people/jasanoff.htm AJ] | ||
| [[User:Xiaosai Yao| XY]] | | [[User:Xiaosai Yao| XY]] | ||
| | | [[20.109(S10):Characterize protein expression (Day6)| Characterize protein expression]] | ||
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| [[User:Xiaosai Yao| XY]] | | [[User:Xiaosai Yao| XY]] | ||
| | | [[20.109(S10):Assay protein behavior (Day7)| Assay protein behavior]] | ||
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| [[User:Xiaosai Yao| XY]] | | [[User:Xiaosai Yao| XY]] | ||
| | | [[20.109(S10):Data analysis (Day8)| Data analysis]] | ||
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Revision as of 17:01, 17 January 2010
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| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 2 | ANS | Orientation | ||||
| 1 | 1 | Thur Feb 4 | CB | Amplify aptamer-encoding DNA | ||
| 1 | 2 | Tue Feb 9 | CB | Purify aptamer-encoding DNA | ||
| 1 | 3 | Thur Feb 11 | CB | Prepare RNA by IVT | ||
| President's Day Feb 15 | No lab Feb 16 or Feb 17 | |||||
|---|---|---|---|---|---|---|
| 1 | 4 | Thur Feb 18 | CB | Purify RNA and run affinity column | ||
| 1 | 5 | Tue Feb 23 | CB | RNA to DNA by RT-PCR | ||
| 1 | 6 | Thur Feb 25 | CB | Module 1 Day 6: Post-selection IVT and journal club | ||
| 1 | 7 | Tue Mar 2 | CB | Aptamer binding assay | ||
| 1 | 8 | Thur Mar 4 | CB | Journal club | ||
| 2 | 1 | Tue Mar 9 | AJ | XY | Start-up protein engineering | |
| 2 | 2 | Thur Mar 11 | AJ | XY | Site-directed mutagenesis | |
| 2 | 3 | Tue Mar 16 | AJ | XY | Bacterial amplification of DNA | |
| 2 | 4 | Thur Mar 18 | AJ | XY | Prepare expression system | |
| Spring Break Mar 22-26 | No lab all week | |||||
| 2 | 5 | Tue Mar 30 | AJ | XY | Induce protein and evaluate DNA | |
| 2 | 6 | Thur Apr 1 | AJ | XY | Characterize protein expression | |
| 2 | 7 | Tue Apr 6 | AJ | XY | Assay protein behavior | |
| 2 | 8 | Thur Apr 8 | AJ | XY | Data analysis | |
| 3 | 1 | Tues Apr 13 | ANS | |||
| 3 | 2 | Thur Apr 15 | ANS | |||
| Patriot's Day April 19-20 | No lab Apr 20 or Apr 21 | |||||
| 3 | 3 | Thur Apr 22 | ANS | |||
| 3 | 4 | Tue Apr 27 | ANS | |||
| 3 | 5 | Thur Apr 29 | ANS | |||
| 3 | 6 | Tue May 4 | ANS | |||
| 3 | 7 | Thur May 6 | ANS | |||
| 3 | 8 | Tue May 11 | ANS | |||
| Thur May 13 | No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD | |||||
