20.109(S10): Spring 2010 schedule: Difference between revisions

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|  
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| [[20.109(S10):Lab tour | Orientation]]
| [[20.109(S10):Lab tour | Orientation]]
|  
| *[[Media:20.109 OrientLect(S10)-given.pdf | Introductory Lecture (PDF)]] <br>*[[Media:S10_Orient_Pre-Lab_ANS.pdf | Intro Pre-Lab, T/R]]
|--
|--
| colspan="7" |
| colspan="7" |
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| [[User:Christina_M_Birch| CB]]
| [[User:Christina_M_Birch| CB]]
| [[20.109(S10):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]]
| [[20.109(S10):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]]
|
|*[[Media:20109_S09-M1Lec1_Niles.pdf | M1D1 Lecture]]<br> *[[Media:S10_M1D1-ANS.pdf | M1D1 Pre-Lab]]
|--
|--
| 1
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| [[User:Christina_M_Birch| CB]]
| [[User:Christina_M_Birch| CB]]
| [[20.109(S10):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]]
| [[20.109(S10):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]]
|
|*[[Media:20109_S10-M1Lec2_Niles.pdf | M1D2 Lecture]]<br> *[[Media:S10_M1D2-ANS.pdf | M1D2 Pre-Lab]]
|--
|-
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| 3
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| [[User:Christina_M_Birch| CB]]
| [[User:Christina_M_Birch| CB]]
| [[20.109(S10):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]]
| [[20.109(S10):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]]
|
|*[[Media:20109_S10-M1Lec3_Niles.pdf | M1D3 Lecture]]<br> *[[Media:S10_M1D3-ANS.pdf | M1D3 Pre-Lab]]
|--
|--
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| [[User:Christina_M_Birch| CB]]
| [[User:Christina_M_Birch| CB]]
| [[20.109(S10):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]]
| [[20.109(S10):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]]
|
|*[[Media:20109_S10-M1Lec4_Niles.pdf | M1D4 Lecture]]<br> *[[Media:S10_M1D4-ANS.pdf | M1D4 Pre-Lab]]
|--
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| [[User:Christina_M_Birch| CB]]
| [[User:Christina_M_Birch| CB]]
| [[20.109(S10):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]]
| [[20.109(S10):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]]
|
|*[[Media:20109_S10-M1Lec5_Niles.pdf | M1D5 Lecture]]<br> *[[Media:S10_M1D5-ANS.pdf | M1D5 Pre-Lab]]
|--
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| [[User:Christina_M_Birch| CB]]
| [[User:Christina_M_Birch| CB]]
| [[20.109(S10):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]]
| [[20.109(S10):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]]
|
| *[[Media:20109_S10-M1Lec6_Niles.pdf | M1D6 Lecture]]<br> *[[Media:S10_M1D6-ANS.pdf | M1D6 Pre-Lab]]
|--
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| [[User:Christina_M_Birch| CB]]
| [[User:Christina_M_Birch| CB]]
| [[20.109(S10):Aptamer binding assay (Day7)| Aptamer binding assay]]
| [[20.109(S10):Aptamer binding assay (Day7)| Aptamer binding assay]]
|
|*[[Media:20109_S10-M1Lec7_Niles.pdf | M1D7 Lecture]]<br> *[[Media:S10_M1D7-ANS.pdf | M1D7 Pre-Lab]]
|--
|--
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| [[User:Christina_M_Birch| CB]]
| [[User:Christina_M_Birch| CB]]
| [[20.109(S10):Journal club (Day8)| Journal club]]  
| [[20.109(S10):Journal club (Day8)| Journal club]]  
|
|*[[Media:20109_S10-M1Lec8_Niles.pdf | M1D8 Lecture]]
|--
|--
| colspan="7" |
| colspan="7" |
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| [[User:Xiaosai Yao| XY]]
| [[User:Xiaosai Yao| XY]]
| [[20.109(S10):Start-up protein engineering (Day1)| Start-up protein engineering]]
| [[20.109(S10):Start-up protein engineering (Day1)| Start-up protein engineering]]
| <font color = CC0000><b>Module 1 Draft Due</b></font color><br>
| <font color = CC0000><b>Module 1 Draft Due</b></font color><br>*[[Media:20109_S10-M2Lec1_Jasanoff.pdf | M2D1 Lecture]]<br> *[[Media:S10_M2D1-ANS.pdf | M2D1 Pre-Lab]]
|--
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| [[User:Xiaosai Yao| XY]]
| [[User:Xiaosai Yao| XY]]
| [[20.109(S10):Site-directed mutagenesis (Day2)| Site-directed mutagenesis]]
| [[20.109(S10):Site-directed mutagenesis (Day2)| Site-directed mutagenesis]]
|
|*[[Media:20109_S10-M2Lec2_Jasanoff.pdf | M2D2 Lecture]]<br> *[[Media:S10_M2D2-ANS.pdf | M2D2 Pre-Lab]]
|--
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| 2
| 2
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| [[User:Xiaosai Yao| XY]]
| [[User:Xiaosai Yao| XY]]
| [[20.109(S10):Bacterial amplification of DNA (Day3)| Bacterial amplification of DNA]]
| [[20.109(S10):Bacterial amplification of DNA (Day3)| Bacterial amplification of DNA]]
|
|*[[Media:20109_S10-M2Lec3_Jasanoff.pdf | M2D3 Lecture]]<br> *[[Media:S10_M2D3-ANS.pdf | M2D3 Pre-Lab]]
|--
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| 2
| 2
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| [[User:Xiaosai Yao| XY]]
| [[User:Xiaosai Yao| XY]]
| [[20.109(S10):Prepare expression system (Day4)| Prepare expression system]]
| [[20.109(S10):Prepare expression system (Day4)| Prepare expression system]]
|
|*[[Media:20109_S10-M2Lec4_Jasanoff.pdf | M2D4 Lecture]]<br> *[[Media:S10_M2D4-ANS.pdf | M2D4 Pre-Lab]]
|--
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| [[User:Xiaosai Yao| XY]]
| [[User:Xiaosai Yao| XY]]
| [[20.109(S10):Induce protein and evaluate DNA (Day5)| Induce protein and evaluate DNA]]
| [[20.109(S10):Induce protein and evaluate DNA (Day5)| Induce protein and evaluate DNA]]
|  
| *[[Media:20109_S10-M2Lec5_Jasanoff.pdf | M2D5 Lecture]]<br> *[[Media:S10_M2D5-ANS.pdf | M2D5 Pre-Lab]]
|--
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| 2
| 2
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| [[User:Xiaosai Yao| XY]]
| [[User:Xiaosai Yao| XY]]
| [[20.109(S10):Characterize protein expression (Day6)| Characterize protein expression]]
| [[20.109(S10):Characterize protein expression (Day6)| Characterize protein expression]]
| <font color = CC0000><b>Module 1 Final Report Due</b></font color><br>
| <font color = CC0000><b>Module 1 Final Report Due</b></font color><br>*[[Media:20109_S10-M2Lec6_Jasanoff.pdf | M2D6 Lecture]]<br> *[[Media:S10_M2D6-ANS.pdf | M2D6 Pre-Lab]]
|--
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| [[User:Xiaosai Yao| XY]]
| [[User:Xiaosai Yao| XY]]
| [[20.109(S10):Assay protein behavior (Day7)| Assay protein behavior]]
| [[20.109(S10):Assay protein behavior (Day7)| Assay protein behavior]]
|
|*[[Media:20109_S10-M2Lec7_Jasanoff.pdf | M2D7 Lecture]]
|--
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| 2
| 2
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| [[User:Xiaosai Yao| XY]]
| [[User:Xiaosai Yao| XY]]
| [[20.109(S10):Data analysis (Day8)| Data analysis]]
| [[20.109(S10):Data analysis (Day8)| Data analysis]]
|
|*[[Media:20109_S10-M2Lec8_Jasanoff.pdf | M2D8 Lecture]]
|--
|--
| colspan="7" |  
| colspan="7" |  
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| Tues Apr 13
| Tues Apr 13
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
|  
| [[User:Nicole_Casasnovas | NC]]
|
| [[20.109(S10):Start-up biomaterials engineering (Day1) | Start-up biomaterials engineering]]
| <font color = CC0000><b>Module 2 Draft Due</b></font color><br>
| <font color = CC0000><b>Module 2 Draft Due</b></font color><br>*[[Media:20109_S10-M3Lec1_Stachowiak.pdf | M3D1 Lecture]]<br>*[[Media:S10_M3D1-ANS.pdf | M3D1 Pre-Lab]]
|--
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| 3
| 3
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| Thur Apr 15
| Thur Apr 15
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
|  
| [[User:Nicole_Casasnovas | NC]]
|
| [[20.109(S10):Initiate cell culture (Day2) | Initiate cell culture]]
|
|*[[Media:20109_S10-M3Lec2_Stachowiak.pdf | M3D2 Lecture]]
|--
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| Thur Apr 22
| Thur Apr 22
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
|  
| [[User:Nicole_Casasnovas | NC]]
|
| [[20.109(S10):Testing cell viability (Day3) | Testing cell viability]]
|
|*[[Media:20109_S10-M3Lec3_Stachowiak.pdf | M3D3 Lecture]]<br>*[[Media:S10_M3D3-ANS.pdf | M3D3 Pre-Lab]]
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| 3
| 3
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| Tue Apr 27
| Tue Apr 27
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
|  
| [[User:Nicole_Casasnovas | NC]]
|
| [[20.109(S10):Preparing cells for analysis (Day4) | Preparing cells for analysis]]
|
|*[[Media:20109_S10-M3Lec4_Stachowiak.pdf | M3D4 Lecture]]<br>*[[Media:S10_M3D4-ANS.pdf | M3D4 Pre-Lab]]
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| Thur Apr 29
| Thur Apr 29
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
|  
| [[User:Nicole_Casasnovas | NC]]
|
| [[20.109(S10):Transcript-level analysis (Day5) | Transcript-level analysis]]
|
|*[[Media:20109_S10-M3Lec5_Stachowiak.pdf | M3D5 Lecture]]<br>*[[Media:S10_M3D5-ANS.pdf | M3D5 Pre-Lab]]
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| 3
| 3
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| Tue May 4
| Tue May 4
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
|  
| [[User:Nicole_Casasnovas | NC]]
|
| [[20.109(S10):Protein-level analysis (Day6) | Protein-level analysis]]
| <font color = CC0000><b>Module 2 Final Report Due</b></font color><br>
| <font color = CC0000><b>Module 2 Final Report Due</b></font color><br>*[[Media:20109_S10-M3Lec6_Stachowiak.pdf | M3D6 Lecture]]<br>*[[Media:S10_M3D6-ANS.pdf | M3D6 Pre-Lab]]
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| Thur May 6
| Thur May 6
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
|  
| [[User:Nicole_Casasnovas | NC]]
|
| [[20.109(S10):Wrap-up analysis (Day7) | Wrap-up analysis]]
|
| <font color = CC0000><b>Module 3 Report Due</b></font color><br>*[http://openwetware.org/images/d/d5/Mod4_presentations.pdf  Atissa's Lecture]<br>*[[Media:S10_M3D7-ANS.pdf | M3D7 Pre-Lab]]
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| Tue May 11
| Tue May 11
| [[User:AgiStachowiak | ANS]]
| [[User:AgiStachowiak | ANS]]
|  
| [[User:Nicole_Casasnovas | NC]]
|
| [[20.109(S10):Student presentations (Day8) | Student presentations]]
|
|
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|
|
| No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
| No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
|
|}
|}

Latest revision as of 10:53, 7 May 2010


20.109(S10): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Spring 2010        Assignments        Lab Basics        OWW Basics       
RNA Engineering        Protein Engineering        Cell-Biomaterial Engineering              

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 2 ANS Orientation * Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
1 1 Thur Feb 4 JN CB Amplify aptamer-encoding DNA * M1D1 Lecture
* M1D1 Pre-Lab
1 2 Tue Feb 9 JN CB Purify aptamer-encoding DNA * M1D2 Lecture
* M1D2 Pre-Lab
1 3 Thur Feb 11 JN CB Prepare RNA by IVT * M1D3 Lecture
* M1D3 Pre-Lab
President's Day Feb 15 No lab Feb 16 or Feb 17
1 4 Thur Feb 18 JN CB Purify RNA and run affinity column * M1D4 Lecture
* M1D4 Pre-Lab
1 5 Tue Feb 23 JN CB RNA to DNA by RT-PCR * M1D5 Lecture
* M1D5 Pre-Lab
1 6 Thur Feb 25 JN CB Post-selection IVT and journal club * M1D6 Lecture
* M1D6 Pre-Lab
1 7 Tue Mar 2 JN CB Aptamer binding assay * M1D7 Lecture
* M1D7 Pre-Lab
1 8 Thur Mar 4 JN CB Journal club * M1D8 Lecture
2 1 Tue Mar 9 AJ XY Start-up protein engineering Module 1 Draft Due
* M2D1 Lecture
* M2D1 Pre-Lab
2 2 Thur Mar 11 AJ XY Site-directed mutagenesis * M2D2 Lecture
* M2D2 Pre-Lab
2 3 Tue Mar 16 AJ XY Bacterial amplification of DNA * M2D3 Lecture
* M2D3 Pre-Lab
2 4 Thur Mar 18 AJ XY Prepare expression system * M2D4 Lecture
* M2D4 Pre-Lab
Spring Break Mar 22-26 No lab all week
2 5 Tue Mar 30 AJ XY Induce protein and evaluate DNA * M2D5 Lecture
* M2D5 Pre-Lab
2 6 Thur Apr 1 AJ XY Characterize protein expression Module 1 Final Report Due
* M2D6 Lecture
* M2D6 Pre-Lab
2 7 Tue Apr 6 AJ XY Assay protein behavior * M2D7 Lecture
2 8 Thur Apr 8 AJ XY Data analysis * M2D8 Lecture
3 1 Tues Apr 13 ANS NC Start-up biomaterials engineering Module 2 Draft Due
* M3D1 Lecture
* M3D1 Pre-Lab
3 2 Thur Apr 15 ANS NC Initiate cell culture * M3D2 Lecture
Patriot's Day April 19-20 No lab Apr 20 or Apr 21
3 3 Thur Apr 22 ANS NC Testing cell viability * M3D3 Lecture
* M3D3 Pre-Lab
3 4 Tue Apr 27 ANS NC Preparing cells for analysis * M3D4 Lecture
* M3D4 Pre-Lab
3 5 Thur Apr 29 ANS NC Transcript-level analysis * M3D5 Lecture
* M3D5 Pre-Lab
3 6 Tue May 4 ANS NC Protein-level analysis Module 2 Final Report Due
* M3D6 Lecture
* M3D6 Pre-Lab
3 7 Thur May 6 ANS NC Wrap-up analysis Module 3 Report Due
*Atissa's Lecture
* M3D7 Pre-Lab
3 8 Tue May 11 ANS NC Student presentations
Thur May 13 No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
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