20.109(S08): Spring 2008 schedule: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (23 intermediate revisions by 2 users not shown) | |||
| Line 16: | Line 16: | ||
| Tue Feb 5 | | Tue Feb 5 | ||
| ANS | | ANS | ||
| | | VL, BE | ||
| [[20.109(S08):Lab tour | Orientation]] | | [[20.109(S08):Lab tour | Orientation]] | ||
| | | Slides are [[Media:20.109_OrientationLect(S08).ppt|here]] (warning: .ppt download) | ||
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
| Line 26: | Line 26: | ||
| Thur Feb 7 | | Thur Feb 7 | ||
| BE | | BE | ||
| | | DW | ||
| [[20.109(S08):DNA engineering/DNA engineering by PCR (Day 1) | Day 1: DNA engineering using PCR]] | | [[20.109(S08):DNA engineering/DNA engineering by PCR (Day 1) | Day 1: DNA engineering using PCR]] | ||
| | | PDF of slides is [[Media:BE109_S08_Mod1-Day1_ENGELWARD.pdf|here]] | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 34: | Line 34: | ||
| Tue Feb 12 | | Tue Feb 12 | ||
| BE | | BE | ||
| | | DW | ||
| [[20.109(S08):DNA engineering/Clean and cut DNA (Day 2) | Day 2: Clean and cut DNA]] | | [[20.109(S08):DNA engineering/Clean and cut DNA (Day 2) | Day 2: Clean and cut DNA]] | ||
| | | PDF of slides is [[Media:BE109_S08_Mod1-Day2_ENGELWARD.pdf|here]] | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 42: | Line 42: | ||
| Thur Feb 14 | | Thur Feb 14 | ||
| BE | | BE | ||
| | | DW | ||
| [[20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3) | Day 3: Agarose gel electrophoresis]] | | [[20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3) | Day 3: Agarose gel electrophoresis]] | ||
| | | PDF of slides is [[Media:BE109_S08_Mod1-Day3_ENGELWARD.pdf|here]] | ||
|-- | |-- | ||
| | | | ||
| Line 55: | Line 55: | ||
| Thur Feb 21 | | Thur Feb 21 | ||
| BE | | BE | ||
| | | DW | ||
| [[20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4) | Day 4: DNA ligation and bacterial transformation]] | | [[20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4) | Day 4: DNA ligation and bacterial transformation]] | ||
| | | PDF of slides is [[Media:BE109_S08_Mod1-Day4_ENGELWARD.pdf|here]] | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 63: | Line 63: | ||
| Tue Feb 26 | | Tue Feb 26 | ||
| BE | | BE | ||
| | | DW | ||
| [[20.109(S08):DNA engineering/Examine candidate clones (Day 5) | Day 5: Examine candidate clones]] | | [[20.109(S08):DNA engineering/Examine candidate clones (Day 5) | Day 5: Examine candidate clones]] | ||
| | | PDF of slides is [[Media:BE109_S08_Mod1-Day5_ENGELWARD.pdf|here]] | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 71: | Line 71: | ||
| Thur Feb 28 | | Thur Feb 28 | ||
| BE | | BE | ||
| | | DW | ||
| [[20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6) | Day 6: Restriction map and tissue culture]] | | [[20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6) | Day 6: Restriction map and tissue culture]] | ||
| | | PDF of slides is [[Media:BE109_S08_Mod1-Day6_ENGELWARD.pdf|here]] | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 79: | Line 79: | ||
| Tue Mar 4 | | Tue Mar 4 | ||
| BE | | BE | ||
| | | DW | ||
| [[20.109(S08):DNA engineering/Lipofection (Day 7) | Day 7: Lipofection]] | | [[20.109(S08):DNA engineering/Lipofection (Day 7) | Day 7: Lipofection]] | ||
| | | PDF of slides is [[Media:BE109_S08_Mod1-Day7_ENGELWARD.pdf|here]] | ||
|-- | |-- | ||
| 1 | | 1 | ||
| Line 87: | Line 87: | ||
| Thur Mar 6 | | Thur Mar 6 | ||
| BE | | BE | ||
| | | DW | ||
| [[20.109(S08):DNA engineering/FACS analysis (Day 8) | Day 8: FACS analysis]] | | [[20.109(S08):DNA engineering/FACS analysis (Day 8) | Day 8: FACS analysis]] | ||
| | | PDF of slides is [[Media:BE109_S08_Mod1-Day8_ENGELWARD.pdf|here]] | ||
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
| Line 97: | Line 97: | ||
| Tue Mar 11 | | Tue Mar 11 | ||
| AJ | | AJ | ||
| | | VL | ||
| [[20.109(S08):Start-up protein engineering (Day1) | Day 1: Start-up protein engineering]] | | [[20.109(S08):Start-up protein engineering (Day1) | Day 1: Start-up protein engineering]] | ||
| | | 'Introduction to the module' handout ''hardcopies'' are available, in class. (Ditto other Mod2 lectures.) | ||
|-- | |-- | ||
| 2 | | 2 | ||
| Line 105: | Line 105: | ||
| Thur Mar 13 | | Thur Mar 13 | ||
| AJ | | AJ | ||
| | | VL | ||
| [[20.109(S08):Site-directed mutagenesis (Day2) | Day 2: Site-directed mutagenesis]] | | [[20.109(S08):Site-directed mutagenesis (Day2) | Day 2: Site-directed mutagenesis]] | ||
| | | For some reason, the 'Rational protein design' handout is not available, despite past claims to the contrary. | ||
|-- | |-- | ||
| 2 | | 2 | ||
| Line 113: | Line 113: | ||
| Tue Mar 18 | | Tue Mar 18 | ||
| AJ | | AJ | ||
| | | VL | ||
| [[20.109(S08):Prepare expression system (Day3) | Day 3: Prepare expression system]] | | [[20.109(S08):Prepare expression system (Day3) | Day 3: Prepare expression system]] | ||
| | | For some reason, the 'Protein expression and purification' handout is not available, despite past claims to the contrary. | ||
|-- | |-- | ||
| 2 | | 2 | ||
| Line 121: | Line 121: | ||
| Thur Mar 20 | | Thur Mar 20 | ||
| AJ | | AJ | ||
| | | VL | ||
| [[20.109(S08):Induce protein expression (Day4) | Day 4: Induce protein expression]] | | [[20.109(S08):Induce protein expression (Day4) | Day 4: Induce protein expression]] | ||
| | | For some reason, the 'Binding and affinity measurements' handout is not available, despite past claims to the contrary. | ||
|-- | |-- | ||
| | | | ||
| Line 134: | Line 134: | ||
| Tue Apr 1 | | Tue Apr 1 | ||
| AJ | | AJ | ||
| | | VL | ||
| [[20.109(S08):Characterize protein expression (Day5) | Day 5: Characterize protein expression]] | | [[20.109(S08):Characterize protein expression (Day5) | Day 5: Characterize protein expression]] | ||
| | | For some reason, the 'Calcium sensors and sensing' handout is not available, despite past claims to the contrary. | ||
|-- | |-- | ||
| 2 | | 2 | ||
| Line 142: | Line 142: | ||
| Thur Apr 3 | | Thur Apr 3 | ||
| AJ | | AJ | ||
| | | VL | ||
| [[20.109(S08):Assay protein behavior (Day6) | Day 6: Assay protein behavior]] | | [[20.109(S08):Assay protein behavior (Day6) | Day 6: Assay protein behavior]] | ||
| | | Please see [[User:Atissa | Attisa's]] user page | ||
|-- | |-- | ||
| 2 | | 2 | ||
| Line 150: | Line 150: | ||
| Tue Apr 8 | | Tue Apr 8 | ||
| AJ | | AJ | ||
| | | VL | ||
| [[20.109(S08):Data analysis (Day7) | Day 7: Data analysis]] | | [[20.109(S08):Data analysis (Day7) | Day 7: Data analysis]] | ||
| | | 'High throughput screening' handout is available in class. | ||
|-- | |-- | ||
| 2 | | 2 | ||
| Line 158: | Line 158: | ||
| Thur Apr 10 | | Thur Apr 10 | ||
| AJ | | AJ | ||
| | | VL | ||
| [[20.109(S08):Student presentations (M2D8)| Day 8: Student presentations]]<br> | | [[20.109(S08):Student presentations (M2D8)| Day 8: Student presentations]]<br> | ||
| | |'"Real-world" protein engineering' handout is available in class. | ||
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
| Line 168: | Line 168: | ||
| Tues Apr 15 | | Tues Apr 15 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | BE | ||
| [[20.109(S08):Start-up biomaterials engineering (Day1)| Day 1: Start-up biomaterials engineering]] | | [[20.109(S08):Start-up biomaterials engineering (Day1)| Day 1: Start-up biomaterials engineering]] | ||
| | | PDF of slides is [[Media:20.109(S08)_M3Lec1.pdf|here]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| Line 176: | Line 176: | ||
| Thur Apr 17 | | Thur Apr 17 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | BE | ||
| [[20.109(S08):Initiate cell culture (Day2)| Day 2: Initiate cell culture]] | | [[20.109(S08):Initiate cell culture (Day2)| Day 2: Initiate cell culture]] | ||
| | | PDF of slides is [[Media:20.109(S08)_M3Lec2.pdf|here]] | ||
|-- | |-- | ||
| | | | ||
| Line 188: | Line 188: | ||
| 3 | | 3 | ||
| Thur Apr 24 | | Thur Apr 24 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]]/NL | ||
| | | BE | ||
| [[20.109(S08):Testing cell viability (Day3)| Module 3 Day 3: Testing cell viability]] | | [[20.109(S08):Testing cell viability (Day3)| Module 3 Day 3: Testing cell viability]] | ||
| | | PDF of slides is [[Media:20.109(S08)_M3Lec3-shrink.pdf|here]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| Line 197: | Line 197: | ||
| Tue Apr 29 | | Tue Apr 29 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | BE | ||
| [[20.109(S08):Preparing cells for analysis (Day4)| Module 3 Day 4: Preparing cells for analysis]] | | [[20.109(S08):Preparing cells for analysis (Day4)| Module 3 Day 4: Preparing cells for analysis]] | ||
| | | PDF of slides is [[Media:20.109(S08)_M3Lec4.pdf|here]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| Line 205: | Line 205: | ||
| Thur May 1 | | Thur May 1 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | BE | ||
| [[20.109(S08):Transcript-level analysis (Day5)| Module 3 Day 5: Transcript-level analysis]] | | [[20.109(S08):Transcript-level analysis (Day5)| Module 3 Day 5: Transcript-level analysis]] | ||
| | | PDF of slides is [[Media:20.109(S08)_M3Lec5-shrunk.pdf|here]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| Line 213: | Line 213: | ||
| Tue May 6 | | Tue May 6 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | BE | ||
| [[20.109(S08):Protein-level analysis (Day6)| Module 3 Day 6: Protein-level analysis]] | | [[20.109(S08):Protein-level analysis (Day6)| Module 3 Day 6: Protein-level analysis]] | ||
| | | PDF of slides is [[Media:20.109(S08)_M3Lec6.pdf|here]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| Line 221: | Line 221: | ||
| Thur May 8 | | Thur May 8 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | BE | ||
| [[20.109(S08):Wrap-up analysis (Day7)| Module 3 Day 7: Wrap-up analysis]] | | [[20.109(S08):Wrap-up analysis (Day7)| Module 3 Day 7: Wrap-up analysis]] | ||
| | | PDF of slides is [[Media:20.109(S08)_M3Lec7-shrink.pdf|here]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| Line 229: | Line 229: | ||
| Tue May 13 | | Tue May 13 | ||
| [[User:AgiStachowiak | ANS]] | | [[User:AgiStachowiak | ANS]] | ||
| | | BE | ||
|[[20.109(S08):Student presentations (Day8)| Module 3 Day 8: Student presentations]] | |[[20.109(S08):Student presentations (Day8)| Module 3 Day 8: Student presentations]] | ||
| | | (Chalkboard lecture) | ||
|-- | |-- | ||
| | | | ||
Latest revision as of 10:02, 23 May 2008
| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 5 | ANS | VL, BE | Orientation | Slides are here (warning: .ppt download) | ||
| 1 | 1 | Thur Feb 7 | BE | DW | Day 1: DNA engineering using PCR | PDF of slides is here |
| 1 | 2 | Tue Feb 12 | BE | DW | Day 2: Clean and cut DNA | PDF of slides is here |
| 1 | 3 | Thur Feb 14 | BE | DW | Day 3: Agarose gel electrophoresis | PDF of slides is here |
| President's Day Feb 18 | No lab Feb 19 or Feb 20 | |||||
|---|---|---|---|---|---|---|
| 1 | 4 | Thur Feb 21 | BE | DW | Day 4: DNA ligation and bacterial transformation | PDF of slides is here |
| 1 | 5 | Tue Feb 26 | BE | DW | Day 5: Examine candidate clones | PDF of slides is here |
| 1 | 6 | Thur Feb 28 | BE | DW | Day 6: Restriction map and tissue culture | PDF of slides is here |
| 1 | 7 | Tue Mar 4 | BE | DW | Day 7: Lipofection | PDF of slides is here |
| 1 | 8 | Thur Mar 6 | BE | DW | Day 8: FACS analysis | PDF of slides is here |
| 2 | 1 | Tue Mar 11 | AJ | VL | Day 1: Start-up protein engineering | 'Introduction to the module' handout hardcopies are available, in class. (Ditto other Mod2 lectures.) |
| 2 | 2 | Thur Mar 13 | AJ | VL | Day 2: Site-directed mutagenesis | For some reason, the 'Rational protein design' handout is not available, despite past claims to the contrary. |
| 2 | 3 | Tue Mar 18 | AJ | VL | Day 3: Prepare expression system | For some reason, the 'Protein expression and purification' handout is not available, despite past claims to the contrary. |
| 2 | 4 | Thur Mar 20 | AJ | VL | Day 4: Induce protein expression | For some reason, the 'Binding and affinity measurements' handout is not available, despite past claims to the contrary. |
| Spring Break Mar 24-28 | No lab all week | |||||
| 2 | 5 | Tue Apr 1 | AJ | VL | Day 5: Characterize protein expression | For some reason, the 'Calcium sensors and sensing' handout is not available, despite past claims to the contrary. |
| 2 | 6 | Thur Apr 3 | AJ | VL | Day 6: Assay protein behavior | Please see Attisa's user page |
| 2 | 7 | Tue Apr 8 | AJ | VL | Day 7: Data analysis | 'High throughput screening' handout is available in class. |
| 2 | 8 | Thur Apr 10 | AJ | VL | Day 8: Student presentations |
'"Real-world" protein engineering' handout is available in class. |
| 3 | 1 | Tues Apr 15 | ANS | BE | Day 1: Start-up biomaterials engineering | PDF of slides is here |
| 3 | 2 | Thur Apr 17 | ANS | BE | Day 2: Initiate cell culture | PDF of slides is here |
| Patriot's Day April 21-22 | No lab Apr 22 or Apr 23 | |||||
| 3 | 3 | Thur Apr 24 | ANS/NL | BE | Module 3 Day 3: Testing cell viability | PDF of slides is here |
| 3 | 4 | Tue Apr 29 | ANS | BE | Module 3 Day 4: Preparing cells for analysis | PDF of slides is here |
| 3 | 5 | Thur May 1 | ANS | BE | Module 3 Day 5: Transcript-level analysis | PDF of slides is here |
| 3 | 6 | Tue May 6 | ANS | BE | Module 3 Day 6: Protein-level analysis | PDF of slides is here |
| 3 | 7 | Thur May 8 | ANS | BE | Module 3 Day 7: Wrap-up analysis | PDF of slides is here |
| 3 | 8 | Tue May 13 | ANS | BE | Module 3 Day 8: Student presentations | (Chalkboard lecture) |
| Thur May 15 | No lab session. Evaluations during lecture, then celebratory lunch, place TBD. | |||||
