20.109(S08):Module 2

Module 2 Day 1: Start-up protein engineering

(Initial reading about CaM-M13? Get calcium titration curve for WT plasmid? Need this day?)

(Students dissect pericam into constituent parts today or next time?)

Module 2 Day 2: Design protein modification

(Protein viewer, further reading about CaM-M13 to choose targets, plans primers for SDM)

Module 2 Day 3: Site-directed mutagenesis

(Staff or students do transformation/miniprep/transformation work? Timing issues, may take another lab day...)

Module 2 Day 4: Characterize protein expression

(IPTG induction, SDS-PAGE)

Module 2 Day 5: Protein binding assays

(Calcium titration curve, fluorescence-based)

Module 2 Day 6: Sequence analysis

(Also lipofect wild-type plasmid to mammalian cells?)

(Or: one day just dedicated to a journal article? Cf. F07Mod2)

Module 2 Day 7: Name

(Microscopy assays with mammalian cells? MATLAB modeling?)

Module 2 Day 8: Student presentations