2-methylbutane (isopentane)

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[[Image:Methylbutane (isopentane).png|thumb|200px|right|2-methyl-butane (aka isopentane) chemical structure]]
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== Description & warning ==
== Description & warning ==
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Methylbutane is used in the lab as an intermediate cooling medium. A setup could be: a tissue sample immersed in methylbutane, itself immersed in liquid nitrogen. This type of cooling is more efficient than direct contact of the sample with liquid nitrogen (-196°C) which evaporates upon contact with a warm object. Less heat is transmitted from the sample to the gaseous nitrogen than from the sample to the liquid methylbutane (-160°C).
Methylbutane is used in the lab as an intermediate cooling medium. A setup could be: a tissue sample immersed in methylbutane, itself immersed in liquid nitrogen. This type of cooling is more efficient than direct contact of the sample with liquid nitrogen (-196°C) which evaporates upon contact with a warm object. Less heat is transmitted from the sample to the gaseous nitrogen than from the sample to the liquid methylbutane (-160°C).
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== See also ==
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Biological samples flash frozen for example with a combination of liquid nitrogen and methylbutane can then be used for storage, cryosection, etc.
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== See also ==
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* [http://main.uab.edu/Sites/ComparativePathology/histopathology/tissuesubmission/cryosectioning/ Cryosectioning protocol including methylbutane freezing]
* [http://en.wikipedia.org/wiki/Isopentane Wikipedia: Ispentane]
* [http://en.wikipedia.org/wiki/Isopentane Wikipedia: Ispentane]
* [http://en.wikipedia.org/wiki/Liquid_nitrogen Wikipedia: Liquid nitrogen]
* [http://en.wikipedia.org/wiki/Liquid_nitrogen Wikipedia: Liquid nitrogen]

Current revision

2-methyl-butane (aka isopentane) chemical structure
2-methyl-butane (aka isopentane) chemical structure

Description & warning

2-methylbutane (aka methylbutane or isopentane) is a hydrophobic liquid with a boiling point at just 28°C. Since it is also highly flammable, care has to be taken that it is used under the hood away from fire to avoid accident.

Lab use

Methylbutane is used in the lab as an intermediate cooling medium. A setup could be: a tissue sample immersed in methylbutane, itself immersed in liquid nitrogen. This type of cooling is more efficient than direct contact of the sample with liquid nitrogen (-196°C) which evaporates upon contact with a warm object. Less heat is transmitted from the sample to the gaseous nitrogen than from the sample to the liquid methylbutane (-160°C).

Biological samples flash frozen for example with a combination of liquid nitrogen and methylbutane can then be used for storage, cryosection, etc.

See also

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