1 normal acid or base (1N) - Revision history

Smoore: /* 1N, 2N HCl */

2012-02-27T21:05:43Z

1N, 2N HCl

←Older revision		Revision as of 21:05, 27 February 2012
Line 26:		Line 26:
	== 1N, 2N HCl ==		== 1N, 2N HCl ==
	1N HCl		1N HCl
-	* 10 ml fuming HCl (37% v/v = ~~10N~~ = ~~10M~~)	+	* 8.3 ml fuming HCl (37% v/v = ~12N = ~12M)
-	* 90 ml H<sub>2</sub>O	+	* 91.7 ml H<sub>2</sub>O

	2N HCl, see also [http://www.ihcworld.com/_protocols/epitope_retrieval/hcl.htm]		2N HCl, see also [http://www.ihcworld.com/_protocols/epitope_retrieval/hcl.htm]
-	* 20 ml fuming HCl (37% v/v = ~~10N~~ = ~~10M~~)	+	* 16.5 ml fuming HCl (37% v/v = ~12N = ~12M)
-	* 80 ml H<sub>2</sub>O	+	* 83.5 ml H<sub>2</sub>O

	== Molarity (M) also a non-standard unit ==		== Molarity (M) also a non-standard unit ==

Jakob Suckale: recipes for 1N 2N HCl

2010-12-08T16:52:49Z

recipes for 1N 2N HCl

←Older revision		Revision as of 16:52, 8 December 2010
Line 1:		Line 1:
-	Biologists are sometimes confused by the non-standard[http://physics.nist.gov/cuu/Units/checklist.html] chemical unit of '''normality N'''. N refers in general to salts while it's most commonly used in the context of acid and bases.	+	Biologists are sometimes confused by the non-standard[http://physics.nist.gov/cuu/Units/checklist.html] chemical unit of '''normality N'''. N refers in general to salts while it's most commonly used in the context of acid and bases. N can refere to either the cation or the anion in a hydrolysis. In the context of acid and bases it is generally assumed that N refers to the proton or the hydroxide ion.

	* 1 M (mol/l) = 1 N for an acid that releases 1 proton* when dissolved in water, e.g. HCL (*monoprotic)		* 1 M (mol/l) = 1 N for an acid that releases 1 proton* when dissolved in water, e.g. HCL (*monoprotic)
Line 24:		Line 24:
	\|}		\|}

		+	== 1N, 2N HCl ==
		+	1N HCl
		+	* 10 ml fuming HCl (37% v/v = 10N = 10M)
		+	* 90 ml H<sub>2</sub>O

-	By the ~~way~~, ''molarity M'' is also a non-standard unit[http://physics.nist.gov/cuu/Units/checklist.html]. Expressing this explicitly as mol/L or mol/m<sup>3</sup> is clearer to readers less versed in current biological customs.	+	2N HCl, see also [http://www.ihcworld.com/_protocols/epitope_retrieval/hcl.htm]
		+	* 20 ml fuming HCl (37% v/v = 10N = 10M)
		+	* 80 ml H<sub>2</sub>O
		+
		+	== Molarity (M) also a non-standard unit ==
		+
		+	For the nitpickers, ''molarity M'' is also a non-standard unit[http://physics.nist.gov/cuu/Units/checklist.html]. Expressing this explicitly as mol/L or mol/m<sup>3</sup> is clearer to readers less versed in current biological customs.

	==See also==		==See also==
	* [http://en.wikipedia.org/wiki/Concentration#Normality Normality section of Concentration page at the Wikipedia]		* [http://en.wikipedia.org/wiki/Concentration#Normality Normality section of Concentration page at the Wikipedia]
	* [http://physics.nist.gov/cuu/Units/units.html International standard units, SI units]		* [http://physics.nist.gov/cuu/Units/units.html International standard units, SI units]
		+	* [http://www.histosearch.com/histonet/Dec03A/RE.HistonetnormalsolutionA.html Example student question & answer]

	[[Category:Chemical]]		[[Category:Chemical]]
	[[Category:Material]]		[[Category:Material]]
	[[Category:Acid]]		[[Category:Acid]]

Jakob Suckale: 1 normal acid (1N) moved to 1 normal acid or base (1N)

2010-12-08T16:45:58Z

1 normal acid (1N) moved to 1 normal acid or base (1N)

←Older revision

Revision as of 16:45, 8 December 2010

Jakob Suckale: table

2010-12-08T16:45:43Z

table

←Older revision		Revision as of 16:45, 8 December 2010
Line 3:		Line 3:
	* 1 M (mol/l) = 1 N for an acid that releases 1 proton* when dissolved in water, e.g. HCL (*monoprotic)		* 1 M (mol/l) = 1 N for an acid that releases 1 proton* when dissolved in water, e.g. HCL (*monoprotic)
	* 1 M (mol/l) = ''2'' N for an acid that releases 2 protons, e.g. H<sub>2</sub>SO<sub>4</sub> (diprotic)		* 1 M (mol/l) = ''2'' N for an acid that releases 2 protons, e.g. H<sub>2</sub>SO<sub>4</sub> (diprotic)
		+
		+	For example, some antigen retrieval methods use 2N hydrochloric acid to open up the tissue to allow antibody binding. This could be a nucleotide analogue, as BrdU or dUTP-TMR in cell proliferation of apoptosis assays.
		+
		+	{\| {{table}} OR border=1 cellpadding=5 cellspacing=0 OR {{sorttable}}
		+	! style="background:lightgrey"\|acid/case
		+	! style="background:lightgrey"\|molecular weight
		+	! style="background:lightgrey"\|N vs M
		+	\|-
		+	\| HCl -> <font color=blue>1·H<sup>+</sup></font> + Cl<sup>-</sup>
		+	\| 36.5 g/mol
		+	\| 1N = 1M = 36.5g/L
		+	\|-
		+	\| H<sub>2</sub>SO<sub>4</sub> -> <font color=blue>2·H<sup>+</sup></font> + SO<sub>4</sub><sup>2-</sup>
		+	\| 98 g/mol
		+	\| 2N = 1M = 98g/L
		+	\|-
		+	\| NaOH -> Na<sup>+</sup> + <font color=blue>1·OH<sup>-</sup></font>
		+	\| 40 g/mol
		+	\| 1N = 1M = 40g/L
		+	\|}
		+

	By the way, ''molarity M'' is also a non-standard unit[http://physics.nist.gov/cuu/Units/checklist.html]. Expressing this explicitly as mol/L or mol/m<sup>3</sup> is clearer to readers less versed in current biological customs.		By the way, ''molarity M'' is also a non-standard unit[http://physics.nist.gov/cuu/Units/checklist.html]. Expressing this explicitly as mol/L or mol/m<sup>3</sup> is clearer to readers less versed in current biological customs.

Jakob Suckale: categories

2010-12-08T16:29:44Z

Jakob Suckale: links

2010-12-08T16:28:43Z

links

←Older revision		Revision as of 16:28, 8 December 2010
Line 4:		Line 4:
	* 1 M (mol/l) = ''2'' N for an acid that releases 2 protons, e.g. H<sub>2</sub>SO<sub>4</sub> (diprotic)		* 1 M (mol/l) = ''2'' N for an acid that releases 2 protons, e.g. H<sub>2</sub>SO<sub>4</sub> (diprotic)

-	By the way, ''molarity M'' is also a non-standard unit[http://physics.nist.gov/cuu/Units/checklist.html]. Expressing this explicitly as mol/L or mol/m<sup>3</sup> is clearer to ~~reader~~ less ~~used to~~ biological customs.	+	By the way, ''molarity M'' is also a non-standard unit[http://physics.nist.gov/cuu/Units/checklist.html]. Expressing this explicitly as mol/L or mol/m<sup>3</sup> is clearer to readers less versed in current biological customs.
		+
		+	==See also==
		+	* [http://en.wikipedia.org/wiki/Concentration#Normality Normality section of Concentration page at the Wikipedia]
		+	* [http://physics.nist.gov/cuu/Units/units.html International standard units, SI units]

Jakob Suckale: molarity

2010-12-08T16:24:51Z

molarity

←Older revision		Revision as of 16:24, 8 December 2010
Line 3:		Line 3:
	* 1 M (mol/l) = 1 N for an acid that releases 1 proton* when dissolved in water, e.g. HCL (*monoprotic)		* 1 M (mol/l) = 1 N for an acid that releases 1 proton* when dissolved in water, e.g. HCL (*monoprotic)
	* 1 M (mol/l) = ''2'' N for an acid that releases 2 protons, e.g. H<sub>2</sub>SO<sub>4</sub> (diprotic)		* 1 M (mol/l) = ''2'' N for an acid that releases 2 protons, e.g. H<sub>2</sub>SO<sub>4</sub> (diprotic)
		+
		+	By the way, ''molarity M'' is also a non-standard unit[http://physics.nist.gov/cuu/Units/checklist.html]. Expressing this explicitly as mol/L or mol/m<sup>3</sup> is clearer to reader less used to biological customs.

Jakob Suckale: definition

2010-12-08T16:24:12Z

definition

←Older revision		Revision as of 16:24, 8 December 2010
Line 1:		Line 1:
	Biologists are sometimes confused by the non-standard[http://physics.nist.gov/cuu/Units/checklist.html] chemical unit of '''normality N'''. N refers in general to salts while it's most commonly used in the context of acid and bases.		Biologists are sometimes confused by the non-standard[http://physics.nist.gov/cuu/Units/checklist.html] chemical unit of '''normality N'''. N refers in general to salts while it's most commonly used in the context of acid and bases.
		+
		+	* 1 M (mol/l) = 1 N for an acid that releases 1 proton* when dissolved in water, e.g. HCL (*monoprotic)
		+	* 1 M (mol/l) = ''2'' N for an acid that releases 2 protons, e.g. H<sub>2</sub>SO<sub>4</sub> (diprotic)

Jakob Suckale: * page created *

2010-12-08T16:23:56Z

*** page created ***

New page

Biologists are sometimes confused by the non-standard[http://physics.nist.gov/cuu/Units/checklist.html] chemical unit of '''normality N'''. N refers in general to salts while it's most commonly used in the context of acid and bases.

←Older revision		Revision as of 16:29, 8 December 2010
Line 9:		Line 9:
	* [http://en.wikipedia.org/wiki/Concentration#Normality Normality section of Concentration page at the Wikipedia]		* [http://en.wikipedia.org/wiki/Concentration#Normality Normality section of Concentration page at the Wikipedia]
	* [http://physics.nist.gov/cuu/Units/units.html International standard units, SI units]		* [http://physics.nist.gov/cuu/Units/units.html International standard units, SI units]
		+
		+	[[Category:Chemical]]
		+	[[Category:Material]]
		+	[[Category:Acid]]