-80 Glycerol Stocks: Difference between revisions
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*add 300μL mixture to each barcode tube | *add 300μL mixture to each barcode tube | ||
*scan each tube on Simple Scan 1000 (on the computer next to the robot) | *scan each tube on Simple Scan 1000 (on the computer next to the robot) | ||
***enter data, and click save | |||
*log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file] | *log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file] | ||
*store in -80 | *store in -80 | ||
Revision as of 10:27, 9 June 2010
Protocol
- add 1 equivalent of 50% glycerol to overnight stock
- add 300μL mixture to each barcode tube
- scan each tube on Simple Scan 1000 (on the computer next to the robot)
- enter data, and click save
- log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
- store in -80
