BME103:T130 Group 2: Difference between revisions
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'''Experimenting With the Connections'''<br> | '''Experimenting With the Connections'''<br> | ||
When we unplugged (part 3) from | When we unplugged (part 3) from the Thermocouple, the machine's display stopped working, but information continued to transmit to the computer. | ||
When we unplugged the white wire that connects | When we unplugged the white wire that connects the Thermocouple to the 16-tube PCR block, the machine's temperature measurement was disabled/no longer kept track of heat readings. | ||
Revision as of 16:10, 1 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine Testing
Experimenting With the Connections When we unplugged (part 3) from the Thermocouple, the machine's display stopped working, but information continued to transmit to the computer. When we unplugged the white wire that connects the Thermocouple to the 16-tube PCR block, the machine's temperature measurement was disabled/no longer kept track of heat readings.
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction
Sample 1: Positive Control (contains cancer DNA template), Tube label: P
(Add your work from Week 3, Part 2 here)
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology
PCR stands for Polymerase Chain Reaction PCR is used to amplify a specific segment of DNA that codes for cancer.
Some basic components involved in a PCR reaction are:
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results(Your group will add the results of your Fluorimeter measurements from Week 4 here)
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