BME103:W930 Group9 l2: Difference between revisions
Line 125: | Line 125: | ||
'''Flourimeter''' | '''Flourimeter instructions'''<br> | ||
1. Place the flourimeter on the table and turn on the blue light. | 1. Place the flourimeter on the table and turn on the blue light.<br> | ||
2. Place provided glass slide on flourimeter track so that the first row of dots is even with the light. | 2. Place provided glass slide on flourimeter track so that the first row of dots is even with the light.<br> | ||
3. Place phone cradle in front of the flourimeter with a smart phone facing perpendicular to the beam of light. (as seen in the picture). | 3. Place phone cradle in front of the flourimeter with a smart phone facing perpendicular to the beam of light. (as seen in the picture). <br> | ||
4. Add two drops of green dye on the dots that are even with the light. | 4. Add two drops of green dye on the dots that are even with the light.<br> | ||
5. Place two drops of DNA sample on top of the green dye. | 5. Place two drops of DNA sample on top of the green dye.<br> | ||
6. Cover the flourimeter and phone by turning the large box over and placing it above both of them. | 6. Cover the flourimeter and phone by turning the large box over and placing it above both of them.<br> | ||
7. Take a picture of the droplet with the camera. | 7. Take a picture of the droplet with the camera.<br> | ||
8. Save the picture and send it to the imageJ operator. | 8. Save the picture and send it to the imageJ operator.<br><br> | ||
Instructions for opening images in imageJ | Instructions for opening images in imageJ<br> | ||
1. Take a picture of the fluorimeter assembly with a smartphone. | 1. Take a picture of the fluorimeter assembly with a smartphone.<br> | ||
2. Transfer the picture to a laptop equipped with imageJ via icloud or email. | 2. Transfer the picture to a laptop equipped with imageJ via icloud or email.<br> | ||
3. Open imageJ and select file, then open. | 3. Open imageJ and select file, then open.<br> | ||
4. Find the file on the computer and select it. | 4. Find the file on the computer and select it.<br> | ||
5. The image is now open and can be analyzed. | 5. The image is now open and can be analyzed.<br> | ||
6. The image can be split into three images (blue, green, and red) for better analysis by selecting: image-color-split channels. | 6. The image can be split into three images (blue, green, and red) for better analysis by selecting: image-color-split channels.<br> | ||
==Research and Development== | ==Research and Development== |
Revision as of 11:20, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR TEAMEveryone has contributed to this project even though there are only two usernames. Every person used these two users to make edits to the wiki. Dr. Haynes said that this would be sufficient enough to give each member full participation credit for this project LAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
The KeyPad will be detachable, and will be connected through the USB connection. Key Features
The key features of the new design include a larger main heating block and a Instructions The new OpenPCR will be assembled and operated almost identical to the old OpenPCR.
ProtocolsMaterials
PCR Protocol a. Enter in the number of parts the heating portion will have(example First cyle, main cycle and last cycle=3). 3. Put .2 micro liters of DNA in each test tube. a. Use one pipet for each transfer do not reuse the pipet. 5. After the mixture is transferred move the tube into the PCR machine.
Flourimeter instructions 1. Place the flourimeter on the table and turn on the blue light. 1. Take a picture of the fluorimeter assembly with a smartphone. Research and DevelopmentBackground on Disease Markers
Primer Design
|