User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/01/24: Difference between revisions
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| Ligation || <font color="blue"><u></font> | | Ligation || <font color="blue"><u></font> | ||
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| 1. E. coli DH5α-T NLS-HIS-STOP/size, | | 1. E. coli DH5α-T NLS-HIS-STOP/size, 25 ng +V0120 (X/P)/3200, 14.1ng || <font color="blue">NLS-HIS-STOP/C-His 3:1 No Colonies</font> | ||
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| 2. E. coli BL-21 NLS-HIS-STOP/size, | | 2. E. coli BL-21 NLS-HIS-STOP/size, 25 ng + V0120 (X/P)/3200, 14.1ng || <font color="blue">NLS-HIS-STOP/C-His 3:1 No Colonies</font> | ||
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| 3. E. coli BL-21 NLS-HIS-STOP/size, | | 3. E. coli BL-21 NLS-HIS-STOP/size, 25 ng + V0120 (X/P)/3200, 14.1ng || <font color="blue">NLS-HIS-STOP/C-His 2:1 No Colonies</font> | ||
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| 4. E. coli BL-21 NLS-HIS-STOP/size, | | 4. E. coli BL-21 NLS-HIS-STOP/size, --- ng + V0120 (X/P)/3200, 14.1ng || <font color="blue">NLS-HIS-STOP/C-His 3:1 </font> | ||
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| 5. V0120 (X/P)/3200)/ 15 ng (Control Plate)|| | | 5. V0120 (X/P)/3200)/ 15 ng (Control Plate)|| | ||
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* The incubation time for the ligation process was 30min at room temperature. | * The incubation time for the ligation process was 30min at room temperature. | ||
* | * Reaction number 1, 30 μL DH5α-Turbo; ice 5 min.; plate on amp agar | ||
* Reaction number 2, 3, 4, 30μL BL21 in 2.0 mL tubes; ice 2 min.; 42°C 90 sec.; add 800 μL SOC medium; shake @ 37°C 25 min.; pellet @ top speed 3 min.; resuspend in 100 μL amp liq. medium; plate on amp agar |
Revision as of 13:49, 25 January 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
01/24/2013Making Standardized DNA Part (NLS-HIS-STOP) NLS-6His-STOP-F1 CTAGAcccaagaaaaagcgcaaggtacaccatcaccaccatcacgcgtaaagctgagACTAGTAGCGGCCGCTGCA 76 NLS-6His-STOP-R1 GCGGCCGCTACTAGTctcagctttacgcgtgatggtggtgatggtgtaccttgcgctttttcttgggT 68 Set up an annealing reaction as follows:
Heat at 100°C for 5 min., remove the entire heat block or water bath from the heat source, and allow to cool slowly to room temperature.
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