LOCUS BBa_V0002_ 2077 bp DNA circular 16-SEP-2005 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|372863384| COMMENT VNTDBDATE|385743668| COMMENT LSOWNER| COMMENT VNTNAME|BBa_V0002_m| COMMENT VNTAUTHORNAME|Caroline Ajo-Franklin| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "BBa_V0002_m" 0 2077 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) COMMENT (CWordArray 47 48 49 50 51 52 81 0 1 2 3 4 82 5 83 6 7 8 9 61 10 11 12 COMMENT 13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 25 26 55 91 COMMENT 27 28 29 30 31 92 32 34 35 33 53 58 36 93 56 94 37 57 38 39 40 41 42 43 COMMENT 95 44 59 99 45 100 96 97 46) (CWordArray) COMMENT (CStringList "EcoRI" "NotI" "PstI" "SpeI" "XbaI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 2077 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 120 0 0 "") COMMENT #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 127 0 149 0 0 "") COMMENT #2=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 159 0 0 "") COMMENT #3=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 141 0 0 "") COMMENT #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 135 0 0 "")) COMMENT (CObList COMMENT #5=(CFSignal (CObList) "VF2" 27 0 0 1 20 0 (CStringList) (CStringList) 1 COMMENT 1 1 1 "1..20") COMMENT #6=(CFSignal (CObList) "P(BLA)" 30 0 1 1990 1990 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2070..2070)") COMMENT #7=(CFSignal (CObList) "AP(R)" 4 0 1 1095 1955 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1175..2035)") COMMENT #8=(CFSignal (CObList) "ORI" 33 0 0 336 336 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "416..416") COMMENT #9=(CFSignal (CObList) "rep (pMB1)" 33 0 1 321 935 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(401..1015)") COMMENT #10=(CFSignal (CObList) "Verification forward" 27 0 0 1998 2017 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2078..2097") COMMENT #11=(CFSignal (CObList) "Verification reverse" 27 0 1 217 236 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(297..316)") COMMENT #12=(CFSignal (CObList) "pSB103 F" 27 0 0 160 179 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "240..259") COMMENT #13=(CFSignal (CObList) "pSB103 R" 27 0 1 50 69 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(50..69)") COMMENT #14=(CFSignal (CObList) "Stem-loop" 39 0 1 90 109 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(90..109)") COMMENT #15=(CFSignal (CObList) "end of terminator" 21 0 1 77 80 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(77..80)") COMMENT #16=(CFSignal (CObList) "A to T mutation" 62 0 0 133 133 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "133..133")) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #17=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #18=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "BBa_V0002_m" 2) 5 COMMENT "" 0 4) COMMENT #19=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'BBa_V0002_m'" 1) COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. Original author: Caroline Ajo-Franklin" COMMENT 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 04/27/05 01:09PM" 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 09/16/05 03:01PM" 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 2077 bp" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #27=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 4-AUG-2004" 1))) COMMENT "Standard Fields") COMMENT #30=(CFolderPar COMMENT (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Caroline Ajo-Franklin" 1))) "Original Author") COMMENT #32=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #33=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #36=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1095 (3 #7# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #37=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1095 End: 1955 (Complementary)" COMMENT 1) COMMENT #38=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "bla gene- Ap(r) determinant" 1) COMMENT #39=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #40=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1175..2035)" 1))) "AP(R)"))) COMMENT "CDS (1 total)") COMMENT #41=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #42=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 77 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 77 End: 80 (Complementary)" COMMENT 1) COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #45=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(77..80)" 1))) COMMENT "end of terminator"))) COMMENT "Misc. Feature (1 total)") COMMENT #46=(CFolderPar COMMENT (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #47=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 20 " 1) COMMENT #49=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1..20" 1))) "VF2") COMMENT #51=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 50 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 50 End: 69 (Complementary)" COMMENT 1) COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(50..69)" 1))) "pSB103 R") COMMENT #55=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 160 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 160 End: 179 " 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 240..259" 1))) "pSB103 F") COMMENT #59=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 217 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 217 End: 236 (Complementary)" COMMENT 1) COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(297..316)" 1))) COMMENT "Verification reverse") COMMENT #63=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1998 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1998 End: 2017" 1) COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2078..2097" 1))) COMMENT "Verification forward"))) "Primer (5 total)") COMMENT #67=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #68=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1990 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1990 End: 1990 (Complementary)" COMMENT 1) COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "bla gene promoter" 1) COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2070..2070)" 1))) COMMENT "P(BLA)"))) "Promoter Prokaryotic (1 total)") COMMENT #73=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #74=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 321 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 321 End: 935 (Complementary)" COMMENT 1) COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(401..1015)" 1))) COMMENT "rep (pMB1)") COMMENT #78=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 336 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 336 End: 336 " 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "RNaseH cleavage point" 1) COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 416..416" 1))) "ORI"))) COMMENT "Replication Origin (2 total)") COMMENT #83=(CFolderPar COMMENT (CGroupPar (CParagraph 39 (7 39 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #84=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 90 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 90 End: 109 (Complementary)" COMMENT 1) COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(90..109)" 1))) COMMENT "Stem-loop"))) "Stem Loop (1 total)") COMMENT #88=(CFolderPar COMMENT (CGroupPar (CParagraph 62 (7 62 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #89=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 133 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 133 End: 133 " 1) COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 133..133" 1))) "A to T mutation"))) COMMENT "Mutation (1 total)"))) "Feature Map") COMMENT #93=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #94=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 3447712 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #95=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #96=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 120 " 1))))) "EcoRI: 1 site") 1 5 COMMENT "GAATTC" "CTTAAG") COMMENT #97=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 3447632 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #98=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #99=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 127 " 1) COMMENT #100=(CLinePar COMMENT (CParagraph 0 (1 #1# 2) 1 2 2 0 191) COMMENT " N2: 149 " 1))))) "NotI: 2 sites") 2 6 COMMENT "GCGGCCGC" "CGCCGGCG") COMMENT #101=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 3447552 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #102=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #103=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 159 " 1))))) "PstI: 1 site") 5 COMMENT 1 "CTGCAG" "GACGTC") COMMENT #104=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 3447472 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #105=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #106=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 141 " 1))))) "SpeI: 1 site") 1 COMMENT 5 "ACTAGT" "TGATCA") COMMENT #107=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 3447392 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #108=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #109=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 135 " 1))))) "XbaI: 1 site") 1 COMMENT 5 "TCTAGA" "AGATCT"))) "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #110=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #111=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #112=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #113=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #114=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #115=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #116=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #117=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #118=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #119=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #120=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #121=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #122=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #123=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #124=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #125=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #126=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #127=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #128=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #129=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #130=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #131=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #132=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #133=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #134=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #135=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #136=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #137=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #138=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #139=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #140=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #141=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #142=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #143=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #144=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #145=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #146=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #147=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #148=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #149=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -5842) 1 1 0 1 1 COMMENT (mapper: 16.8195 -23.209 40.0378 40.0378 0.01 10 -1 2077 2077 1 0 0) COMMENT #150=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -114 100) COMMENT (CObjectList COMMENT #151=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -923 100) COMMENT (CObjectList COMMENT #152=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #116# 23927268 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27721 6.27521 1 0.0214037) 0.302031) COMMENT #153=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #113# 3408600 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "BBa V0002 m" "@N" 1 0 0.871165 0 COMMENT -10 7.54287 1.44863 Nil) COMMENT #154=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #112# -777 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "2077 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 3.12205 0.949103 Nil)) (CObjectList)) COMMENT #155=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -183 100) COMMENT (CObjectList COMMENT #156=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -393 100) COMMENT (CObjectList COMMENT #157=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #114# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 0.368478 2.96897 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #158=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 268634488 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AP{\\super r}" COMMENT "@N" 1 -14.2406 -1.74233 0 -10 1.64844 COMMENT 1.12394 #157#)) (CObjectList)) COMMENT #159=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil 23920700 100) COMMENT (CObjectList COMMENT #160=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #119# 342 100) COMMENT (LOGPEN 0 8 13395507) 10 1 6.04364 1.9 COMMENT 0.082322 1) COMMENT #161=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 3408528 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "end of terminator" "@N" 1 7.68793 17.4233 0 COMMENT -10 7.71771 1.12394 #160#)) (CObjectList)) COMMENT #162=(CGroupWidget COMMENT (CWidget 27 (7 27 0) 1 2 0 0 Nil -393 100) COMMENT (CObjectList COMMENT #163=(CPrimerArrow COMMENT (CLine COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #117# 23285812 COMMENT 100) (LOGPEN 0 6 6723840) 8 1.16464 6.21278 COMMENT 6.27319) 0.4 0.082322 1.8 1.8 0) COMMENT #164=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 3408464 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "VF2" "@N" 1 COMMENT 2.46983 20.908 0 -10 1.72337 1.12394 #163#) COMMENT #165=(CPrimerArrow COMMENT (CLine COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #117# 3473300 COMMENT 100) (LOGPEN 0 6 6723840) 8 1.16464 COMMENT 0.181219 0.241625) 0.4 0.082322 1.8 1.8 0) COMMENT #166=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 23937804 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification forward" "@N" 1 -7.62967 COMMENT 13.9386 0 -10 8.71676 1.12394 #165#) COMMENT #167=(CPrimerArrow COMMENT (CLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #117# 23333756 COMMENT 100) (LOGPEN 0 6 6723840) 8 0.835356 COMMENT 5.56039 5.6208) 0.4 0.082322 1.8 1.8 1) COMMENT #168=(CLabel (CWidget 0 (0 0) 1 2 0 0 #111# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification reverse" "@N" 1 17.4562 0 0 -10 COMMENT 8.84164 1.12394 #167#) COMMENT #169=(CPrimerArrow COMMENT (CLine COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 6 6723840) 8 1.16464 5.73255 COMMENT 5.79296) 0.4 0.082322 1.8 1.8 0) COMMENT #170=(CLabel (CWidget 0 (0 0) 1 2 0 0 #111# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "pSB103 F" "@N" COMMENT 1 15.3592 1.74233 0 -10 4.29594 1.12394 #169#) COMMENT #171=(CPrimerArrow COMMENT (CLine COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 6 6723840) 8 0.835356 6.06478 COMMENT 6.12519) 0.4 0.082322 1.8 1.8 1) COMMENT #172=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 3408512 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "pSB103 R" "@N" COMMENT 1 5.5984 19.1656 0 -10 4.42082 1.12394 #171#)) COMMENT (CObjectList)) COMMENT #173=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -738 100) COMMENT (CObjectList COMMENT #174=(CPromoterArrow COMMENT (CLine COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #133# 27089108 COMMENT 100) (LOGPEN 0 4 128) 6 0.835356 0.265787 COMMENT 0.353054) 0.4 0.082322 0.915356 1.8 1.8 1) COMMENT #175=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# -108 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "P(BLA)" "@N" 1 COMMENT -7.83261 12.1963 0 -10 3.172 1.12394 #174#)) COMMENT (CObjectList)) COMMENT #176=(CGroupWidget COMMENT (CWidget 33 (7 33 0) 1 2 0 0 Nil -114 100) COMMENT (CObjectList COMMENT #177=(CScratch COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #119# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.26138 1.9 COMMENT 0.082322 1) COMMENT #178=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 27363956 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "ORI" "@N" 1 COMMENT 14.157 -1.74233 0 -10 1.7983 1.12394 #177#) COMMENT #179=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #114# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 0.835356 3.44919 COMMENT 5.30669 1 0.082322) 0.8 1.8 1) COMMENT #180=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 26727052 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "rep (pMB1)" COMMENT "@N" 1 15.1362 -5.22699 0 -10 5.04523 1.12394 COMMENT #179#)) (CObjectList)) COMMENT #181=(CGroupWidget COMMENT (CWidget 39 (7 39 0) 1 2 0 0 Nil -599 100) COMMENT (CObjectList COMMENT #182=(CLine COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #132# 338 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 5.94397 COMMENT 6.00438) COMMENT #183=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 23920476 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem-loop" "@N" COMMENT 1 7.08347 15.681 0 -10 4.59566 1.12394 #182#)) COMMENT (CObjectList)) COMMENT #184=(CGroupWidget COMMENT (CWidget 62 (7 62 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList COMMENT #185=(CScratch COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #119# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.8745 1.9 COMMENT 0.082322 1) COMMENT #186=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #111# 3408528 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "A to T mutation" "@N" 1 12.1197 10.454 0 -10 COMMENT 6.71865 1.12394 #185#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #187=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList COMMENT #188=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList COMMENT #189=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #118# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.91377 1.9 COMMENT 0.082322 1) COMMENT #190=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #120# 3408536 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Eco}RI (120)" "@N (@S)" 5 7.83529 COMMENT 13.9386 0 -10 4.42082 0.949103 #189#)) COMMENT (CObjectList)) COMMENT #191=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList COMMENT #192=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #118# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.79598 1.9 COMMENT 0.082322 1) COMMENT #193=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #120# 3408568 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Pst}I (159)" "@N (@S)" 5 14.8535 COMMENT 3.48466 0 -10 3.62158 0.949103 #192#)) COMMENT (CObjectList)) COMMENT #194=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList COMMENT #195=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #118# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.85034 1.9 COMMENT 0.082322 1) COMMENT #196=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #120# 3408568 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Spe}I (141)" "@N (@S)" 5 13.5892 COMMENT 6.96932 0 -10 3.79641 0.949103 #195#)) COMMENT (CObjectList)) COMMENT #197=(CGroupWidget COMMENT (CWidget 1 (10 #4# 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList COMMENT #198=(CScratch COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #118# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.86846 1.9 COMMENT 0.082322 1) COMMENT #199=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #120# 23964520 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xba}I (135)" "@N (@S)" 5 12.6195 COMMENT 8.71165 0 -10 3.92129 0.949103 #198#)) COMMENT (CObjectList)) COMMENT #200=(CGroupWidget COMMENT (CWidget 2 (10 #1# 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList COMMENT #201=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #118# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.89263 1.9 COMMENT 0.082322 1) COMMENT #202=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #110# 3408536 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Not}I (127)" "@N (@S)" 5 8.21592 COMMENT 12.1963 0 -10 3.84637 0.949103 #201#) COMMENT #203=(CScratch COMMENT (CWidget 2 (1 #1# 2) 1 2 0 0 #118# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.82618 1.9 COMMENT 0.082322 1) COMMENT #204=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #110# 3408288 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Not}I (149)" "@N (@S)" 5 14.4507 COMMENT 5.22699 0 -10 3.84637 0.949103 #203#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #205=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList) (CObjectList)) COMMENT #206=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 3408296 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 1944749780 20766784 (CObList))) FEATURES Location/Qualifiers primer 1..20 /vntifkey="27" /label=VF2 promoter complement(1990..1990) /vntifkey="30" /label=P(BLA) /note="bla gene promoter" CDS complement(1095..1955) /vntifkey="4" /label=AP(R) /note="bla gene- Ap(r) determinant" rep_origin 336..336 /vntifkey="33" /label=ORI /note="RNaseH cleavage point" rep_origin complement(321..935) /vntifkey="33" /label=rep\(pMB1) primer 1998..2017 /vntifkey="27" /label=Verification\forward primer complement(217..236) /vntifkey="27" /label=Verification\reverse primer 160..179 /vntifkey="27" /label=pSB103\F primer complement(50..69) /vntifkey="27" /label=pSB103\R stem_loop complement(90..109) /vntifkey="39" /label=Stem-loop misc_feature complement(77..80) /vntifkey="21" /label=end\of\terminator mutation 133..133 /vntifkey="62" /label=A\to\T\mutation BASE COUNT 540 a 518 c 492 g 527 t ORIGIN 1 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga 121 attcgcggcc gcatctagaa ctagtagcgg ccgctgcagg cttcctcgct cactgactcg 181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 1021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1081 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 1141 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 1201 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 1261 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 1321 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 1381 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 1441 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 1501 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 1561 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 1621 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 1681 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 1741 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 1801 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 1861 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 1921 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 1981 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 2041 aaacaaatag gggttccgcg cacatttccc cgaaaag //