DOC

MAtrix gradient for 6 plates

Assuming 8 tubes of the row signals are placed in T2 1+8
Bacteria in T1 1+6
Empty deepwell at S1 and S2
empty plates at P1-P6

ENDDOC

REAGENT M9 TR3 1 W1000 8










#################################

SCRIPT

%Set reader temprature
READER_SET_TEMP 37
INC_TEMP 2 37


% Sugar gradient 1:3
SERIAL_DILUTION M9 T2 1x8 P1 A1+96 135 67.5 WBTA MIX:7x150

% Sugar gradient 1:3
SERIAL_DILUTION M9 T2 2x8 P2 A1+96 135 67.5 WBTA MIX:7x150

% Sugar gradient 1:3
SERIAL_DILUTION M9 T2 3x8 P3 A1+96 135 67.5 WBTA MIX:7x150

% Sugar gradient 1:3
SERIAL_DILUTION M9 T2 4x8 P4 A1+96 135 67.5 WBTA MIX:7x150

% Sugar gradient 1:3
SERIAL_DILUTION M9 T2 5x8 P5 A1+96 135 67.5 WBTA MIX:7x150

% Sugar gradient 1:3
SERIAL_DILUTION M9 T2 6x8 P6 A1+96 135 67.5 WBTA MIX:7x150


% Bac dilution
DIST_REAGENT M9 S1 A1+24 590 W1000 TIPTYPE:1000,TIPMODE:KEEPTIP
TRANSFER_WELLS T1 1x24 S1 A1+24 10 W1000 TIPTYPE:1000,MIX:6x500

SHAKER_START 400
WAIT 120
SHAKER_STOP

%Bac to plate
MPDIST S1 A1+8 P1 A1+48 15 W200
MPDIST S1 A1+8 P1 A7+48 15 W200

MPDIST S1 A1+8 P2 A1+48 15 W200
MPDIST S1 A1+8 P2 A7+48 15 W200

MPDIST S1 A2+8 P3 A1+48 15 W200
MPDIST S1 A2+8 P3 A7+48 15 W200

MPDIST S1 A2+8 P4 A1+48 15 W200
MPDIST S1 A2+8 P4 A7+48 15 W200

MPDIST S1 A3+8 P5 A1+48 15 W200
MPDIST S1 A3+8 P5 A7+48 15 W200

MPDIST S1 A3+8 P6 A1+48 15 W200
MPDIST S1 A3+8 P6 A7+48 15 W200


%Oil to Plate
MPDIST TR2 1+8 P1 A1+48 100 OIL1000 TIPTYPE:1000
MPDIST TR2 1+8 P1 A7+48 100 OIL1000 TIPTYPE:1000

MPDIST TR2 1+8 P2 A1+48 100 OIL1000 TIPTYPE:1000
MPDIST TR2 1+8 P2 A7+48 100 OIL1000 TIPTYPE:1000

MPDIST TR2 1+8 P3 A1+48 100 OIL1000 TIPTYPE:1000
MPDIST TR2 1+8 P3 A7+48 100 OIL1000 TIPTYPE:1000

MPDIST TR2 1+8 P4 A1+48 100 OIL1000 TIPTYPE:1000
MPDIST TR2 1+8 P4 A7+48 100 OIL1000 TIPTYPE:1000

MPDIST TR2 1+8 P5 A1+48 100 OIL1000 TIPTYPE:1000
MPDIST TR2 1+8 P5 A7+48 100 OIL1000 TIPTYPE:1000

MPDIST TR2 1+8 P6 A1+48 100 OIL1000 TIPTYPE:1000
MPDIST TR2 1+8 P6 A7+48 100 OIL1000 TIPTYPE:1000



MOVE_PLATE P1 I21 1
MOVE_PLATE P2 I22 1
MOVE_PLATE P3 I23 1
MOVE_PLATE P4 I24 1
MOVE_PLATE P5 I25 1
MOVE_PLATE P6 I26 

INC_TEMP 2 37




LOOP 200

%Read plate1
MOVE_PLATE I21 R1
INC_CLOSE 2
INC_START 2 6
READER_MEASURE
INC_STOP 2
MOVE_PLATE R1 I21 1

%Read plate2
MOVE_PLATE I22 R1
INC_CLOSE 2
INC_START 2 6
READER_MEASURE
INC_STOP 2
MOVE_PLATE R1 I22 1

%Read plate3
MOVE_PLATE I23 R1
INC_CLOSE 2
INC_START 2 6
READER_MEASURE
INC_STOP 2
MOVE_PLATE R1 I23 1

%Read plate4
MOVE_PLATE I24 R1
INC_CLOSE 2
INC_START 2 6
READER_MEASURE
INC_STOP 2
MOVE_PLATE R1 I24 1

%Read plate5
MOVE_PLATE I25 R1
INC_CLOSE 2
INC_START 2 6
READER_MEASURE
INC_STOP 2
MOVE_PLATE R1 I25 1

%Read plate6
MOVE_PLATE I26 R1
INC_CLOSE 2
INC_START 2 6
READER_MEASURE
INC_STOP 2
MOVE_PLATE R1 I26 1

ENDLOOP


%








ENDSCRIPT