LOCUS pKn002 4250 bp DNA circular 2-JUL-2008 DEFINITION ATCC 37253: very usable general purpose vector. KEYWORDS ATCC. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|486748429| COMMENT VNTDBDATE|486829538| COMMENT LSOWNER| COMMENT VNTNAME|pKn002| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTAUTHOREML|mgethers@mit.edu| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers promoter complement(4085..4085) /vntifkey="30" /label=P(BLA) /note=" bla gene promoter " CDS complement(3193..4050) /vntifkey="4" /label=AP(R) /note=" bla gene- Ap(r) determinant " promoter complement(2071..2071) /vntifkey="30" /label=P(LAC) /note=" lac promoter " rep_origin 2431..2431 /vntifkey="33" /label=ORI /note=" RNaseH cleavage point " misc_feature 2041..2064 /vntifkey="21" /label=M13\Reverse\Primer\Binding\Site misc_feature 352..375 /vntifkey="21" /label=M13\Forward\Primer\Binding\Site misc_feature 411..1980 /vntifkey="21" /label=HmgR\Upstream\Fragment misc_feature 1659..1677 /vntifkey="21" /label=BT1032 misc_feature 1429..1446 /vntifkey="21" /label=BT1033 BASE COUNT 933 a 1258 c 1152 g 907 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagatag 421 cgatggacct tgctcgccgg catccccaca tgctccgcca ggcgcgacag cgaggtcgcc 481 ggggagagtt cggcgagggc cttgaggata tcggtaccga cttcggcgga gcggaccttc 541 tgcttgccgg aggtttcggc gggactgctg ttcttttcca tggacgcggg ttcctgcggg 601 cggggccgac gttatagctt gacgctgcct tccactcaaa ttacgttatg cgtaattgaa 661 ttacgataaa aataacgcag ccagcgtgcc aggtccccgg cggatcggcg gcaagctgcc 721 cccactcccc ggaggcctca gatgaacctc gactccactg ccctcgccta tcaatcgggc 781 ttcggcaacg aattcagcag cgaagcgctc cccggcgccc tgccggtcgg ccagaactcc 841 ccgcagaaag cgccctacgg cctgtacgcc gaactgctct ccggcaccgc cttcaccatg 901 gctcgcagcg aggcccggcg cacctggctg taccgcatca cgccgtcggc caagcatccg 961 ccgttccgcc gcctggaacg acagatcgcc ggtgccgaac tggatgcgcc gactcccaac 1021 cgcctgcgct gggacccgct ggcactgccc gagcagccca ccgacttcct cgacggcctg 1081 ctgcgcatgg ccgccaacgc gcccggcgac aagcccgccg gcgtgagcat ctaccagtac 1141 ctggccaacc gctcgatgga gcgttgcttc tacgacgccg atggcgaact gctgctggtc 1201 ccgcaattgg gccgtctgcg cctgtgcacc gaactcggcg cgctgcaagt cgaaccgctg 1261 gagatcgcgg tgatcccgcg cgggatgaag ttccgcgtcg agctgctcga cggcgaggca 1321 cgcggctata tcgccgagaa ccacggcgcg ccgctgcgcc tgcccgacct cggcccgatc 1381 ggcagcaatg gcctggccaa tccgcgcgac ttcctgaccc cggtggcgcg ctacgaggac 1441 agccgccagc cgctgcaact ggtgcagaaa tacctcggcg agctgtgggc ctgcgagctt 1501 gaccactcgc cgctggacgt ggtcgcctgg cacggcaaca acgtgcccta caagtacgac 1561 ctgcgccgct tcaacaccat cggcacggtc agcttcgacc acccggaccc gtcgatcttc 1621 accgtgctga cctcccccac cagcgtcccc ggcctggcca acatcgactt cgtgatcttc 1681 ccgccgcgct ggatggtggc cgagaacacc ttccgtccgc catggttcca ccgcaacctg 1741 atgaacgaat tcatgggcct gatccagggc gcctatgacg ccaaggccgg cggcttcgtg 1801 cccggcggcg cctcgctgca cagttgcatg agcgcccacg gcccggacgc ggaaagctgc 1861 gacaaggcca tcgccgccga cctcaagccg cacaggatcg accagaccat ggccttcatg 1921 ttcgagacca gccaggtcct ccggccgagc cgtgccgccc tcgagacgcc ggccctgcag 1981 gtcgactcta gaggatcccc gggtaccgag ctcgaattcg taatcatggt catagctgtt 2041 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 2101 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 2161 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 2221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 2281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 2401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 2461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 2521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 2581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 2641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 2701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 2761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 2821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 2881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 2941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 3001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 3061 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 3121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 3181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 3241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 3301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 3361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 3421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 3481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 3541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 3601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 3661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 3721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 3781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 3841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 3901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 3961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 4021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 4081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 4141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 4201 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc //