LOCUS               3016 bp ds-DNA     circular     21-MAR-2007
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(1139..1821)
                     /ApEinfo_label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             complement(1919..2578)
                     /ApEinfo_label=AmpR
                     /ApEinfo_fwdcolor=#f8c887
                     /ApEinfo_revcolor=#f8c887
     misc_feature    7..14
                     /ApEinfo_label=NotI
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    968..975
                     /ApEinfo_label=NotI
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    16..21
                     /ApEinfo_label=XbaI
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
     misc_feature    961..966
                     /ApEinfo_label=SpeI
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
     misc_feature    975..980
                     /ApEinfo_label=PstI
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
     misc_feature    1..6
                     /ApEinfo_label=EcoRI
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
     misc_feature    831..959
                     /ApEinfo_label=dblTerm
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    103..819
                     /ApEinfo_label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    23..76
                     /ApEinfo_label=P_tet of R0040
                     /ApEinfo_fwdcolor=#dfd0df
                     /ApEinfo_revcolor=#dfd0df
ORIGIN
        1 gaattcgcgg ccgcatctag agtccctatc agtgatagag attgacatcc ctatcagtga
       61 tagagatact gagcactact agagaaagag gagaaatact agatgcgtaa aggagaagaa
      121 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      181 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      241 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
      301 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
      361 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      421 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      481 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      541 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
      601 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      661 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
      721 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
      781 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca
      841 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      901 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      961 actagtagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1561 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2881 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2941 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     3001 ctaaggatga tttctg    
//