LOCUS pORE_R1 9295 bp ds-DNA circular 18-JUN-2010 DEFINITION Binary Vector pORE_R1, complete sequence. ACCESSION AY562544 VERSION AY562544.1 GI:45862381 KEYWORDS . SOURCE Binary vector pORE_R1 ORGANISM Binary vector pORE_R1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9342) AUTHORS Coutu,C.M., Brandle,J., Brown,K., Simmonds,J., Brown,D., Mici,B. and Hegedus,D.D. TITLE pORE: a new series of binary vectors with a removable selectable marker and 21 site MCS JOURNAL Unpublished REFERENCE 2 (bases 1 to 9342) AUTHORS Coutu,C.M., Brandle,J., Brown,K., Simmonds,J., Brown,D., Mici,B. and Hegedus,D.D. TITLE Direct Submission JOURNAL Submitted (01-MAR-2004) Molecular Genetics, Agriculture and Agrifood Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source join(1..4760,6645..9295) /organism="Binary vector pORE_R1" /mol_type="other DNA" /db_xref="taxon:267421" /lab_host="Agrobacterium tumefaciens" /label=source:Binary vector pORE_R1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink terminator 1..253 /note="NOS terminator; polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; derived from pBI121 in GenBank Accession Number AF485783" /label=NOS terminator; polyadenylation signal from nopali /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_recomb 260..307 /note="FRT site; excision of selectable marker cassette from transformed plants using FLP recombinase" /label=FRT site; excision of selectable marker cassette f /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 356..3610 /note="derived from pCB301 in GenBank Accession Number AF139061; used SDM to remove NotI, XhoI, EcoRV and SfiI sites" /label=derived from pCB301 in GenBank Accession Number AF /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 356..516 /note="T-DNA left border" /label=T-DNA left border /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin 523..1152 /note="RK2 oriV" /label=RK2 oriV /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS 1364..2158 /note="neomycin-kanamycin phosphotransferase type III" /codon_start=1 /transl_table=11 /product="NptIII" /protein_id="AAS78963.1" /db_xref="GI:45862382" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" /label=NptIII /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS 2457..3605 /note="derived from plasmid RK2" /codon_start=1 /transl_table=11 /product="TrfA" /protein_id="AAS78964.1" /db_xref="GI:45862383" /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVG SMVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKK RKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALF TTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPI TFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLD RGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKL ETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" /label=TrfA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin 3663..4543 /note="ColE1; high copy number origin of replication" /label=ColE1; high copy number origin of replication /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4549..4711 /note="T-DNA right border" /label=T-DNA right border /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind 4724..4746 /note="M13/pUC forward" /PCR_conditions="60 degree C annealing temperature" /label=M13/pUC forward /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4747..4760 /note="multiple cloning site; selected unique sites: SfoI-SacII-HindIII-NheI-MfeI-PspOMI-AclI-XhoI-XbaI-StuI- BamHI-SmaI-EcoRI-SalI-NotI-ClaI-PstI-SacI" /label=multiple cloning site; selected unique sites: SfoI /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink primer_bind complement(6656..6678) /note="M13/pUC reverse" /PCR_conditions="60 degree C annealing temperature" /label=M13/pUC reverse /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink terminator 6685..6937 /note="NOS terminator; polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; derived from pBI121 in GenBank Accession Number AF485783" /label=NOS terminator; polyadenylation signal from nopali(1) /ApEinfo_label=NOS terminator; polyadenylation signal from nopali /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_recomb 6944..6991 /note="FRT site; excision of selectable marker cassette from transformed plants using FLP recombinase" /label=FRT site; excision of selectable marker cassette f(1) /ApEinfo_label=FRT site; excision of selectable marker cassette f /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 7008..8484 /note="At4g15440 promoter; hydroperoxide lyase promoter; constitutive; high level expression of transgenes in dicots; active in Arabidopsis, tobacco, cauliflower, soybean, alfalfa, peach, white spruce, wheat, and grape; in Arabidopsis, expression has been detected in all tissues but the petals, style, and pollen; removed ClaI, SfoI, XhoI and MfeI sites" /label=At4g15440 promoter; hydroperoxide lyase promoter; /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS 8493..9287 /note="neomycin phosphotransferase II; removed SfoI, PstI, and NcoI sites; repaired reduction-in-function mutation; derived from pBI121 in GenBank Accession Number AF485783" /codon_start=1 /transl_table=11 /product="NptII" /protein_id="AAS78966.1" /db_xref="GI:45862385" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /label=NptII /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4766..4814 /label=biobrick MCS /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 61 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 121 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 181 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 241 atgttactag atccctaggg aagttcctat tccgaagttc ctattctctg aaaagtatag 301 gaacttcttt gcgtattggg cgctcttggc ctttttggcc accggtcgta cggttaaaac 361 caccccagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt 421 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca 481 caaaatcacc actcgataca ggcagcccat cagtccacta gacgctcacc gggctggttg 541 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga 601 agccgtgtgc gagacaccgc agccgccggc gttgtggata cctcgcggaa aacttggccc 661 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt 721 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa 781 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga 841 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat 901 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg 961 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc 1021 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg 1081 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc 1141 tcccggcccg ctctcgcgtt ggcagcatca cccataattg tggtttcaaa atcggctccg 1201 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt 1261 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt 1321 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat 1381 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat 1441 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac 1501 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg 1561 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag 1621 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca 1681 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga 1741 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt 1801 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa 1861 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt 1921 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt 1981 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg 2041 gtcgatcagg gaggatattg gggaagaaca gtatgtcgag ctattttttg acttactggg 2101 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta 2161 cctagatgtg gcgcaacgat gccggcgaca agcaggagcg caccgacttc ttccgcatca 2221 agtgttttgg ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat 2281 tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga 2341 ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc 2401 aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga 2461 atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg 2521 aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt 2581 ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg 2641 ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg 2701 aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga 2761 agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag caagccgcgt 2821 tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc 2881 cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca 2941 cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca 3001 aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt 3061 ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt 3121 tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg 3181 aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc 3241 acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt 3301 cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca 3361 cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact 3421 atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg 3481 gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt 3541 tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac 3601 gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct ttactgagat 3661 cctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4261 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 4441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 4501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttggatctc ctgtggttgg 4561 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta 4621 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat 4681 agtttaaact gaaggcggga aacgacaatc tgctagtgga tctcccagtc acgacgttgt 4741 aaaacgggcg ccccgcggaa AGCTTGAATT CGCGGCCGCT TCTAGAATTA GAGGACTAGT 4801 AGCGGCCGCT GCAGAGAGCT CATGTTACGT CCTGTAGAAA CCCCAACCCG TGAAATCAAA 4861 AAACTCGACG GCCTGTGGGC ATTCAGTCTG GATCGCGAAA ACTGTGGAAT TGATCAGCGT 4921 TGGTGGGAAA GCGCGTTACA AGAAAGCCGG GCTATTGCTG TGCCAGGCAG TTTTAACGAT 4981 CAGTTCGCCG ATGCAGATAT TCGTAATTAT GCGGGCAACG TCTGGTATCA GCGCGAAGTC 5041 TTTATACCGA AAGGTTGGGC AGGCCAGCGT ATCGTGCTGC GTTTCGATGC GGTCACTCAT 5101 TACGGCAAAG TGTGGGTCAA TAATCAGGAA GTGATGGAGC ATCAGGGCGG CTATACGCCA 5161 TTTGAAGCCG ATGTCACGCC GTATGTTATT GCCGGGAAAA GTGTACGTAT CACCGTTTGT 5221 GTGAACAACG AACTGAACTG GCAGACTATC CCGCCGGGAA TGGTGATTAC CGACGAAAAC 5281 GGCAAGAAAA AGCAGTCTTA CTTCCATGAT TTCTTTAACT ATGCCGGAAT CCATCGCAGC 5341 GTAATGCTCT ACACCACGCC GAACACCTGG GTGGACGATA TTACCGTGGT GACGCATGTC 5401 GCGCAAGACT GTAACCACGC TTCTGTTGAC TGGCAGGTGG TGGCCAATGG TGATGTCAGC 5461 GTTGAACTGC GTGATGCGGA TCAACAGGTG GTTGCAACTG GACAAGGCAC TAGCGGGACT 5521 TTGCAAGTGG TGAATCCGCA CCTCTGGCAA CCGGGTGAAG GTTATCTCTA TGAACTGTGC 5581 GTCACAGCCA AAAGCCAGAC AGAGTGTGAT ATTTACCCGC TTCGCGTCGG CATCCGGTCA 5641 GTGGCAGTGA AGGGCGAACA GTTCCTGATT AACCACAAAC CGTTCTACTT TACTGGCTTT 5701 GGTCGTCATG AAGATGCGGA CTTGCGTGGC AAAGGATTCG ATAACGTGCT GATGGTGCAC 5761 GACCACGCAT TAATGGACTG GATTGGGGCC AACTCCTACC GTACCTCGCA TTACCCTTAC 5821 GCTGAAGAGA TGCTCGACTG GGCAGATGAA CATGGCATCG TGGTGATTGA TGAAACTGCT 5881 GCTGTCGGCT TTAACCTCTC TTTAGGCATT GGTTTCGAAG CGGGCAACAA GCCGAAAGAA 5941 CTGTACAGCG AAGAGGCAGT CAACGGGGAA ACTCAGCAAG CGCACTTACA GGCGATTAAA 6001 GAGCTGATAG CGCGTGACAA AAACCACCCA AGCGTGGTGA TGTGGAGTAT TGCCAACGAA 6061 CCGGATACCC GTCCGCAAGG TGCACGGGAA TATTTCGCGC CACTGGCGGA AGCAACCCGT 6121 AAACTCGACC CGACCCGTCC GATCACCTGC GTCAATGTAA TGTTCTGCGA CGCTCACACC 6181 GATACCATCA GCGATCTCTT TGATGTGCTG TGCCTGAACC GTTATTACGG ATGGTATGTC 6241 CAAAGCGGCG ATTTGGAAAC GGCAGAGAAG GTACTGGAAA AAGAACTTCT GGCCTGGCAG 6301 GAGAAACTGC ATCAGCCGAT TATCATCACC GAATACGGCG TGGATACGTT AGCCGGGCTG 6361 CACTCAATGT ACACCGACAT GTGGAGTGAA GAGTATCAGT GTGCATGGCT GGATATGTAT 6421 CACCGCGTCT TTGATCGCGT CAGCGCCGTC GTCGGTGAAC AGGTATGGAA TTTCGCCGAT 6481 TTTGCGACCT CGCAAGGCAT ATTGCGCGTT GGCGGTAACA AGAAAGGGAT CTTCACTCGC 6541 GACCGCAAAC CGAAGTCGGC GGCTTTTCTG CTGCAAAAAC GCTGGACTGG CATGAACTTC 6601 GGTGAAAAAC CGCAGCAGGG AGGCAAACAA TGAGGTACCT TTTGctagtg atatccctgt 6661 gtgaaattgt tatccgctac gcgtgatcgt tcaaacattt ggcaataaag tttcttaaga 6721 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag 6781 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga 6841 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat 6901 aaattatcgc gcgcggtgtc atctatgtta ctagatccca tgggaagttc ctattccgaa 6961 gttcctattc tctgaaaagt ataggaactt cagcgatcgc agacgtcaac gtggatactt 7021 ggcagtggtt acttggcttt tcctttattt tcttttggac ggaagcggtg gttactttgt 7081 cacacattta aaaaaacacg tgtttctcac ttttttctat tcccgtcaca aacaatttta 7141 agaaagatcc atctatcgtg atctttctat caaacaaaag aaaaaaggtc ttcatagtaa 7201 cgctacaaca tcaaatatgt ggttgctctg acatcagtcg ggaaaataag gatatggcgg 7261 cattggccac atctattggg gtcccaactt cctttcacaa aaaaattaaa ttgggtgtcc 7321 caacttttat ctttgatata gtgacatgag tatcgggagc attggacaat ggataaaatg 7381 agaactaaaa aaattctggt taatttttga tcattgttat ttaaaaggtt attttatcta 7441 taatctaccc atattgatca gttttattta aatttgttta gctaccgctc cacgagagag 7501 atcctcatct taaaaatgga atatggaaat tacacacgac cccaaaagta tattttttct 7561 ctggagaatg ctatttagag ctttgactat atggtctgaa ttagaaagac gggaaataaa 7621 atctgctaag tgatataagc tctaagtagg cgatgtgtga tggagaacac cttttcttta 7681 acagtcttca tgttttacag attcgcgaac ttcgaatatc cctatacggt ctgtctaacc 7741 ctcgtgtgtc ttttgagtcc aagataaagg ccattattga gtaacataga catgctggaa 7801 tccaaccatt gaagtcacaa ctgtccatgt agattctttg gagaatctga aaagtcttaa 7861 taaaggtggt gtttcaaaga aaacaaaaca aatgagttaa gaaaaaaaaa tatcatgtag 7921 tggtcgagta ttatgttatt tattgtgtag ctaccaatct ttattcttta aatctgacat 7981 aaaatgctac aaacttttta cctcgtctat agccccaaaa aacctaacca cggttctaaa 8041 accacacaca gtgattttgg ttgacgacaa tgcctctcct tcctcaaaac gatttattta 8101 cattttttaa atcaaatgtt acattttata ccataattaa gtctttttac agaatactta 8161 gatggaagag atgtataaaa aaggaggaaa ttgtaaaaaa catatttcga tcaattaaac 8221 caggattcat aaaaatataa gtatatatat aaatgatgtt tcgtttagcg atgaacttca 8281 ctcatatgat aatacttaac aatataagta cataaaaaat aaaataaaat taattgttta 8341 cgaaaagtct acaaatactg catgtataat taatgttctc tttatttatt tatttatacc 8401 ttaccaagat atatctataa ccgcatagaa atagaaggcg aagagataat ttccaaaaac 8461 aagaaaaacc tctaagctca aaagggccgg ccatgattga acaagatgga ttgcacgcag 8521 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 8581 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gaggccggtt ctttttgtca 8641 agaccgacct gtccggtgcc ctgaatgaac ttcaagacga ggcagcgcgg ctatcgtggc 8701 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 8761 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 8821 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 8881 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 8941 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 9001 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg actcatggcg 9061 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 9121 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 9181 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 9241 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaggc gcgcc //