LOCUS 20109_pMax_BFP_S 3508 bp ds-DNA linear 16-MAR-2014 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments=Ligation of : pmax_mOrange [2781 nt] : (SacI[1685] / NheI[9 53]) to PCR(ZDN_pmax_BFP) [719 nt] : (NheI[5] / SacI[728]) COMMENT SerialCloner_Ends=0,0,,0, COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 1..798 /SerialCloner_Color=&hFF8019 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc= immediate early promoter of cytomegalovirus (PCMV IE) \ /label=PCMV IE /ApEinfo_fwdcolor=#ff65e7 /ApEinfo_revcolor=#ff65e7 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 811..947 /SerialCloner_Color=&hB3B3B3 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=False /SerialCloner_Desc=chimeric intron for enhanced gene expression \ /label=Chimeric intron /ApEinfo_fwdcolor=#7a1cff /ApEinfo_revcolor=#7a1cff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_site 1684..1884 /SerialCloner_Color=&hB3B3B3 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=False /SerialCloner_Desc=SV40 late mRNA polyadenylation signal \ /label=SV40 poly A /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 1958..2599 /SerialCloner_Color=&hFFFF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=False /SerialCloner_Desc=pUC plasmid replication origin \ /label=pUC ori /ApEinfo_fwdcolor=#ffe374 /ApEinfo_revcolor=#ffe374 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(2661..3452) /SerialCloner_Color=&hFF00FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=Kanamycin resistance gene \ /label=KANr /ApEinfo_fwdcolor=#c5924f /ApEinfo_revcolor=#c5924f /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter complement(3453..3484) /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=Bacterial promoter for expression of Kanr gene \ /label=P /ApEinfo_fwdcolor=#f240ff /ApEinfo_revcolor=#f240ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 959..964 /label=original ScaI /ApEinfo_fwdcolor=#ffd232 /ApEinfo_revcolor=#ffd232 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 953..1681 /label=PCR product cloned by Zac /ApEinfo_fwdcolor=#42ffa1 /ApEinfo_revcolor=#42ffa1 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 974..1675 /SerialCloner_Color=&h0004C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /label=tagBFP /ApEinfo_fwdcolor=#0059ff /ApEinfo_revcolor=#0059ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg 721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat 781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt 841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg 901 cacctattgg tcttactgac atccactttg cctttctctc cacaggggta cCGctagCAG 961 TACTGCTGCC ACCATGAGCG AGCTGATTAA GGAGAACATG CACATGAAGC TGTACATGGA 1021 GGGCACCGTG GACAACCATC ACTTCAAGTG CACATCCGAG GGCGAAGGCA AGCCCTACGA 1081 GGGCACCCAG ACCATGAGAA TCAAGGTGGT CGAGGGCGGC CCTCTCCCCT TCGCCTTCGA 1141 CATCCTGGCT ACTAGCTTCC TCTACGGCAG CAAGACCTTC ATCAACCACA CCCAGGGCAT 1201 CCCCGACTTC TTCAAGCAGT CCTTCCCTGA GGGCTTCACA TGGGAGAGAG TCACCACATA 1261 CGAAGACGGG GGCGTGCTGA CCGCTACCCA GGACACCAGC CTCCAGGACG GCTGCCTCAT 1321 CTACAACGTC AAGATCAGAG GGGTGAACTT CACATCCAAC GGCCCTGTGA TGCAGAAGAA 1381 AACACTCGGC TGGGAGGCCT TCACCGAGAC GCTGTACCCC GCTGACGGCG GCCTGGAAGG 1441 CAGAAACGAC ATGGCCCTGA AGCTCGTGGG CGGGAGCCAT CTGATCGCAA ACATCAAGAC 1501 CACATATAGA TCCAAGAAAC CCGCTAAGAA CCTCAAGATG CCTGGCGTCT ACTATGTGGA 1561 CTACAGACTG GAAAGAATCA AGGAGGCCAA CAACGAGACC TACGTCGAGC AGCACGAGGT 1621 GGCAGTGGCC AGATACTGCG ACCTCCCTAG CAAACTGGGG CACAAGCTTA ATTGAGagct 1681 cgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat 1741 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa 1801 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa 1861 gtaaaacctc tacaaatgtg gtacttaaga gggggagacc aaagggcgag acgttaaggc 1921 ctcacgtgac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 1981 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 2041 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 2101 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 2161 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 2221 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 2281 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 2341 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 2401 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 2461 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccacgctggt 2521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 2581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2641 attttggtca tgccgtctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 2701 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 2761 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc 2821 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 2881 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 2941 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 3001 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 3061 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 3121 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 3181 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 3241 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 3301 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 3361 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 3421 gaacctgcgt gcaatccatc ttgttcaatc ataatattat tgaagcattt atcagggttc 3481 gtctcgtccc ggtctcctcc catgcatg //