LOCUS pRML1 9742 bp DNA circular 3-OCT-2006 DEFINITION Complementary copy of pRML1. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|423242946| COMMENT VNTDBDATE|423342136| COMMENT VNTNAME|pRML1| COMMENT VNTAUTHORNAME|Thomas Knight| COMMENT VNTAUTHORTEL|617 253 7807| COMMENT VNTAUTHORFAX|617 258 7413| COMMENT VNTAUTHOREML|tk@mit.edu| COMMENT VNTAUTHORAD1|MIT CSAIL 32-312| COMMENT VNTAUTHORAD2|32 Vassar St| COMMENT VNTAUTHORAD3|Cambridge, MA 01239| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers misc_feature complement(7038..8972) /vntifkey="21" /label=pBluescript\II\SK+\region misc_difference 7409..7409 /vntifkey="85" /label=A\in\pBluescript\II\SK+ telomere 9097..9723 /vntifkey="42" /label=Telomere\from\pYAC4 misc_feature 4744..7035 /vntifkey="21" /label=TK\cassette\from\Herpesvirus centromere 650..761 /vntifkey="5" /label=Centromere,\Yeast\chromosome\4 misc_feature 100..956 /vntifkey="21" /label=Centromere\region,\yeast\chromosome\4 misc_feature 1544..3607 /vntifkey="21" /label=ARS,\Trp1\region\of\chromosome\4 promoter complement(961..1416) /vntifkey="29" /label=Gal1\Promoter misc_feature 4005..4744 /vntifkey="21" /label=fragment\of\His3\gene CDS 7166..8026 /vntifkey="4" /label=ampR CDS 6224..7090 /vntifkey="4" /label=Glycoprotein\H\precursor,\Herpesvirus CDS 4804..5934 /vntifkey="4" /label=Thymidine\kinase,\Herpesvirus CDS complement(2224..2898) /vntifkey="4" /label=Trp1\gene misc_feature 654..661 /vntifkey="21" /label=CDE\I misc_feature 662..739 /vntifkey="21" /label=CDE\II misc_feature 740..750 /vntifkey="21" /label=CDE\III misc_feature 1548..2386 /vntifkey="21" /label=ARS1 misc_feature 1416..3007 /vntifkey="21" /label=from\pYAC4 misc_feature 102..956 /vntifkey="21" /label=from\pYAC4 misc_feature 4005..4751 /vntifkey="21" /label=from\pYAC4 primer complement(50..69) /vntifkey="27" /label=T3\promoter BASE COUNT 2487 a 2301 c 2376 g 2578 t ORIGIN 1 atcgatttcg aagctagctc gcgagtcgac cgcggccgca gatctgaatt ccctttagtg 61 agggttaatt tacgtagcga tgataagctg tcaaacatga gctgggccat tctcatgaag 121 aatatcttga atttattgtc atattactag ctagttggtg tggaagtcct aatatcggtg 181 atcaatatag tggttgacat gctggctagt caacattgag ccttttgatc atgcaaatat 241 attacggtat tttacaatca aatatcaaac ttaactattg actttataac ttatttaggt 301 ggtaacattc ttataaaaaa gaaaaaaatt actgcaaaac agtactagct tttaacttgt 361 atcctaggtt atctatgctg tctcaccata gagaatatta cctatttcag aatgtatgtc 421 catgattcgc cgggtaaata catataatac acaaatctgg cttaataaag tctataatat 481 atctcataaa gaagtgctaa attggctagt gctatatatt tttaagaaaa tttcttttga 541 ctaagtccat atcgactttg taaaagttca cattagcata catatattac acgagccaga 601 aatagtaact tttgcctaaa tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat 661 gcttataatc aactttttta aaaatttaaa atactttttt attttttatt tttaaacata 721 aatgaaataa tttatttatt gtttatgatt accgaaacat aaaacctgct caagaaaaag 781 aaactgtttt gtccttggaa aaaaagcact acctaggagc ggccaaaatg ccgaggcttt 841 catagcttaa actctttaca gaaaataggc attatagatc agttcgagtt ttcttattct 901 tccttccggt tttatcgtca cagttttaca gtaaataagt atcacctctt agagttcgac 961 ccggggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata 1021 cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt attagttaaa 1081 gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca tcgcttcgct 1141 gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta 1201 atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat 1261 aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca 1321 tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccttcg 1381 gagggctgtc acccgctcgg cggcttctaa tccgtactat gagataagca agtatcatct 1441 catttcatta cctgaagtcg agtaaacaga aaatccaatt gttgatgaac ctcaatgact 1501 tagaactatc tatcggcaga tcatataaag aggatttaga attaattcca catgttaaaa 1561 tagtgaagga gcatgttcgg cacacagtgg accgaacgtg gggtaagtgc actagggtcc 1621 ggttaaacgg atctcgcatt gatgaggcaa cgctaattat caacatatag attgttatct 1681 atctgcatga acacgaaatc tttacttgac gacttgaggc tgatggtgtt tatgcaaaga 1741 aaccactgtg tttaatatgt gtcactgttt gatattactg tcagcgtaga agataatagt 1801 aaaagcggtt aataagtgta tttgagataa gtgtgataaa gtttttacag cgaaaagacg 1861 ataaatacaa gaaaatgatt acgaggatac ggagagaggt atgtacatgt gtatttatat 1921 actaagctgc cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg 1981 tagcgtatgc gcctgtgaac attctcttca acaagtttga ttccattgcg gtgaaatggt 2041 aaaagtcaac cccctgcgat gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt 2101 tagcattatc tttacatctt gttattttac agattttatg tttagatctt ttatgcttgc 2161 ttttcaaaag gcctgcaggc aagtgcacaa acaatactta aataaatact actcagtaat 2221 aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt 2281 tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc 2341 aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg ggctctcttg 2401 ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc 2461 tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt 2521 gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc 2581 ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc 2641 caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga 2701 actattttta tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt 2761 tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat ctagagcaca 2821 ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt 2881 gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt 2941 gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt 3001 aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa ggtgacaagc 3061 tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact gcaaagtaca 3121 catatattac gatgctgttc tattaaatgc ttcctatatt atatatatag taatgtcgtt 3181 aaccgttgat ttttactgat tggtctctgc cgtgcgggtg agcggcgcgt ccagacgggc 3241 gggtgcacgt ttctttttct gaccatttcc ctgtcagctc ttttagatcg gggctgccgt 3301 ttgaaaagtt ttatcatcgc cagacatgcc ataaggaata tctccaccgc ctctaactgt 3361 ccctaactcg gtctctttct tataactacc attactcagt gttgagagat tggtagaaat 3421 atacgcgtat acgttcattc aagacttatt ttcagccagt aatattccct actcctcttc 3481 cgttttcaga atagagatgg accagccaag gacccattca ggacccacga cggccagcaa 3541 tcctgccccc agctccacca actcgtcatc tgctcccagc gcgactaatt caaagcagga 3601 aagatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc 3661 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg 3721 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg 3781 tcggaatgga cgataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg 3841 ttaatgtcat gataataatg gtttcttaga atttaattcg aacacgcaga tgcagtcggg 3901 gcggcgcggt cccaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaataccga 3961 gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatct ctcttgcgag 4021 atgatcccgc attttcttga aagctttgca gaggctagca gaattaccct ccacgttgat 4081 tgtctgcgag gcaagaatga tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt 4141 gccataagag aagccacctc gcccaatggt accaacgatg ttccctccac caaaggtgtt 4201 cttatgtagt gacaccgatt atttaaagct gcagcatacg atatatatac atgtgtatat 4261 atgtatacct atgaatgtca gtaagtatgt atacgaacag tatgatactg aagatgacaa 4321 ggtaatgcat cattctatac gtgtcattct gaacgaggcg cgctttcctt ttttcttttt 4381 gctttttctt tttttttctc ttgaactcga gaaaaaaaat ataaaagaga tggaggaacg 4441 ggaaaaagtt agttgtggtg ataggtggca agtggtattc cgtaagaaca acaagaaaag 4501 catttcatat tatggctgaa ctgagcgaac aagtgcaaaa tttaagcatc aacgacaaca 4561 acgagaatgg ttatgttcct cctcacttaa gaggaaaacc aagaagtgcc agaaataaca 4621 gtagcaacta caataacaac aacggcggct acaacggtgg ccgtggcggt ggcagcttct 4681 ttagcaacaa ccgtcgtggt ggttacggca acggtggttt cttcggtgga aacaacggtg 4741 gcagcagatc ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg 4801 cgtatggctt cgtacccctg ccatcaacac gcgtctgcgt tcgaccaggc tgcgcgttct 4861 cgcggccata acaaccgacg tacggcgttg cgccctcgcc ggcaacaaaa agccacggaa 4921 gtccgcctgg agcagaaaat gcccacgcta ctgcgggttt atatagacgg tccccacggg 4981 atggggaaaa ccaccaccac gcaactgctg gtggccctgg gttcgcgcga cgatatcgtc 5041 tacgtacccg agccgatgac ttactggcgg gtgttggggg cttccgagac aatcgcgaac 5101 atctacacca cacaacaccg cctcgaccag ggtgagatat cggccgggga cgcggcggtg 5161 gtaatgacaa gcgcccagat aacaatgggc atgccttatg ccgtgaccga cgccgttctg 5221 gctcctcata tcggggggga ggctgggagc tcacatgccc cgcccccggc cctcaccctc 5281 atcttcgacc gccatcccat cgccgccctc ctgtgctacc cggccgcgcg ataccttatg 5341 ggcagcatga ccccccaggc cgtgctggcg ttcgtggccc tcatcccgcc gaccttgccc 5401 ggcacaaaca tcgtgttggg ggcccttccg gaggacagac acatcgaccg cctggccaaa 5461 cgccagcgcc ccggcgagcg gcttgacctg gctatgctgg ccgcgattcg ccgcgtttat 5521 gggctgcttg ccaatacggt gcggtatctg cagggcggcg ggtcgtggcg ggaggattgg 5581 ggacagcttt cgggggcggc cgtgccgccc cagggtgccg agccccagag caacgcgggc 5641 ccacgacccc atatcgggga cacgttattt accctgtttc gggcccccga gttgctggcc 5701 cccaacggcg acctgtataa cgtgtttgcc tgggctttgg acgtcttggc caaacgcctc 5761 cgtcccatgc acgtctttat cctggattac gaccaatcgc ccgccggctg ccgggacgcc 5821 ctgctgcaac ttacctccgg gatggtccag acccacgtca ccaccccagg ctccataccg 5881 acgatctgcg acctggcgcg cacgtttgcc cgggagatgg gggaggctaa ctgaaacacg 5941 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 6001 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 6061 tcgatacccc accgagaccc cattgggacc aatacgcccg cgtttcttcc ttttccccac 6121 cccaaccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaagcc 6181 ctgccatagc cacgggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg 6241 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca 6301 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa 6361 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct 6421 ggcgccgccg gacgaactaa acctgactac ggcatctctg ccccttcttc gctggtacga 6481 ggagcgcttt tgttttgtat tggtcaccac ggccgagttt ccgcgggacc ccggccagct 6541 gctttacatc ccgaagacct acctgctcgg ccggcccccg aacgcgagcc tgcccgcccc 6601 caccacggtc gagccgaccg cccagcctcc cccctcggtc gcccccctta agggtctctt 6661 gcacaatcca gccgcctccg tgttgctgcg ttcccgggcc tgggtaacgt tttcggccgt 6721 ccctgacccc gaggccctga cgttcccgcg gggagacaac gtggcgacgg cgagccaccc 6781 gagcgggccg cgtgatacac cgcccccccg accgccggtt ggggcccggc ggcacccgac 6841 gacggagctg gacatcacgc acctgcacaa cgcgtccacg acctggttgg ccacccgggg 6901 cctgttgaga tccccaggta ggtacgtgta tttctccccg tcggcctcga cgtggcccgt 6961 gggcatctgg acgacggggg agctggtgct cgggtgcgat gccgcgctgg tgcgcgcgcg 7021 ctacgggcgg gaattaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 7081 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 7141 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 7201 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 7261 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 7321 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 7381 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg 7441 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 7501 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 7561 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 7621 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 7681 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 7741 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 7801 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 7861 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 7921 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 7981 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 8041 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 8101 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 8161 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 8221 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 8281 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 8341 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 8401 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 8461 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 8521 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 8581 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 8641 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 8701 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 8761 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 8821 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 8881 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 8941 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct 9001 gtgcggtatt tcacaccgca tacggtaccc ggggatccat atgactagtg atcctctaga 9061 gtcgatcgac ctgcaggcat gcctgcaggt cgaggcgata agcttcattt ttagataaaa 9121 tttattaatc atcattaatt tcttgaaaaa cattttattt attgatcttt tataacaaaa 9181 aacccttcta aaagtttatt tttgaatgaa aaacttataa aaatttatga aaactacaaa 9241 aaataaaatt tttaattaaa ataattttga taagaacttc aatctttgac tagcttagtc 9301 atttttgaga tttaattaat attttatgtt tattcatata taaactattc aaaatattat 9361 agaatttaaa cattttaaca tcttaatcat tcataaataa ctaaaaatca aagtattaca 9421 ttcaataaat aacttttact caatgtcaaa gaaattattg gggttggggt tggggttggg 9481 gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg 9541 gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg 9601 gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tgggggttgg 9661 ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg ttggggttgg 9721 ggtctgcctc gcgcggaaat tc //