20.109 available enzymes that don't cut M13K07 The names of restriction enzymes that don't cut M13K07 and are available in the 20.109 teaching lab are listed on the left of this list. Right after the name of each enzyme is the sequence of the restriction site in parenthesis. Only the "top" strand is shown, listed in the 5' to 3' direction. The place where the backbone is cut by the enzyme is indicated with a ^. The corresponding cut on the bottom strand is indicated by an underscore. Compare the information here to what is shown in the NEB catalog or ask for help if you need more clarification about what's listed. AatII (G_ACGT^C) Acc65I (G^GTAC_C) AflII (C^TTAA_G) ApaI (G_GGCC^C) AvrII (C^CTAG_G) BclI (T^GATC_A) BglII (A^GATC_T) BmgBI (CAC|GTC) BmtI (G_CTAG^C) BsiWI (C^GTAC_G) BspEI (T^CCGG_A) BssHII (G^CGCG_C) BstZ17I (GTA|TAC) EagI (C^GGCC_G) EcoRI (G^AATT_C) EcoRV (GAT|ATC) FspI (TGC|GCA) HpaI (GTT|AAC) KasI (G^GCGC_C) KpnI (G_GTAC^C) MfeI (C^AATT_G) MluI (A^CGCG_T) NarI (GG^CG_CC) NcoI (C^CATG_G) NheI (G^CTAG_C) PmlI (CAC|GTG) PspOMI (G^GGCC_C) PvuII (CAG|CTG) SalI (G^TCGA_C) ScaI (AGT|ACT) SfoI (GGC|GCC) SpeI (A^CTAG_T) SphI (G_CATG^C) StuI (AGG|CCT) XbaI (T^CTAG_A) ZraI (GAC|GTC)