LOCUS       Exported                2393 bp ds-DNA    circular SYN 16-FEB-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pFL_U1U2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Feb 16, 2014 from SnapGene Viewer 2.2.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1777..1793
                     /note="M13R"
     misc_feature    1917..1922
                     /note="EcoRI MfeI mix"
     misc_feature    1923..1928
                     /note="BfuAI"
     misc_feature    1931..1938
                     /note="AscI"
     misc_feature    1939..1978
                     /note="U1"
     misc_feature    1979..1980
                     /note="Untitled"
     misc_feature    1981..2001
                     /note="BBprefix"
     misc_feature    2068..2087
                     /note="BB suffix"
     misc_feature    2088..2089
                     /note="Untitled"
     misc_feature    2090..2129
                     /note="U2"
     misc_feature    2130..2131
                     /note="Untitled"
     misc_feature    2144
                     /note="lMauBI"
     misc_feature    2145..2184
                     /note="UX"
     misc_feature    2185..2186
                     /note="Untitled"
     misc_feature    2198..2203
                     /note="NsiI PstI mix"
     primer_bind     complement(2372..2389)
                     /note="M13F(-21)"
ORIGIN
        1 tgggcctttc ttcggtagaa aatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag
      661 ccaggtgatt acatttaggt cctcaagaaa ggcccacccg aaggtgagcc aggtgattac
      721 atttgggccc tcattaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      781 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgatg cgggagggct
      841 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      901 tatcagcaat aaaccagcca gccgggaggg ccgagcgcag aagtgatcct gcaactttat
      961 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1021 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1081 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1141 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgccagaagt aagttggccg
     1201 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1261 tgagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1321 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1381 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg taaactctca aggatcttac
     1441 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1501 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1561 gaataagggc gacacggaaa tgttgaatac tcattttagc ttccttagct cctgaaaatc
     1621 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1681 ctcttacgtg ccgatcaagt caaaagcctc cggtcggagg cttttgactt tctgctatgg
     1741 aggtcaggta tgatttaaat ggtcagtatt gagcctcagg aaacagctat gacatcaagc
     1801 tgactagata atctagctga tcgtggaccg atcatacgta taatgccgta agatcacggg
     1861 tcggacgaca gctcgctcgt ccagaactga tcgacactgc tcgatccgct cgcaccgaat
     1921 tgacctgcat ggcgcgccca ttactcgcat ccattctcag gctgtctcgt ctcgtctccg
     1981 gaattcgcgg ccgcttctag agtggatcca tccatgggtg ctagctaaag cttagctcga
     2041 gtgggtaccg taccggtaga gatctgaact agtagcggcc gctgcagccg ctgggagttc
     2101 gtagacggaa acaaacgcag aatccaagcc gcgcgcggtc ttcgccagga tacatagatt
     2161 accacaactc cgagcccttc cacccgccgg cgagaccatg cagggatcga cgagagcagc
     2221 gcgactggat ctgtcgcccg tctcaaacgc aaccctccgg cggtcgcata tcattcagga
     2281 cgagcctcag actccagcgt aactggactg caatcaactc actggctcac cttccggtcc
     2341 acgatcagct agaatcaagc tgactagata aactggccgt cgttttacac ggg
//