LOCUS pBca9525_Bjc0005 4253 bp ds-DNA circular 07-FEB-2012 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 4042..4059 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(2013..2033) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 4075..4174 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1850..1869) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 377..1006 /label=toxR> /ApEinfo_fwdcolor=#9f009f /ApEinfo_revcolor=#9f009f /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4183..4204 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1719..1724,1739..1740) /label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1877..1976 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 728..733 /label=MfeI /ApEinfo_fwdcolor=#8fc2c1 /ApEinfo_revcolor=#8fc2c1 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2245..3255 /label=specR /ApEinfo_fwdcolor=#f2f09d /ApEinfo_revcolor=#f2f09d /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6..345 /label=P_rffGH /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=#ff8080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3269..3935 /label=colE1 /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=#c0c0c0 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 377..451 /label=ToxR prepro /ApEinfo_fwdcolor=#7d0d82 /ApEinfo_revcolor=#7d0d82 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1993..2012) /label=T7 Universal /ApEinfo_fwdcolor=#b19943 /ApEinfo_revcolor=#b19943 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 452..1006 /label=ToxR active peptide /ApEinfo_fwdcolor=#8b52ad /ApEinfo_revcolor=#8b52ad /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1013..1771 /label=cI /ApEinfo_fwdcolor=#80ff00 /ApEinfo_revcolor=#80ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4253..4253 /label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1773..1777 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4244..4249 /label=EcoRI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3258..3263 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2105..2110 /label=XbaI /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(2587..2592,2607..2608) /label=AcuI/Eco57I(1) /ApEinfo_label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(join(3449..3450,3465..3470)) /label=AcuI/Eco57I(2) /ApEinfo_label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GATCTCTCAT TAATAAACTG GCAATGACCA AGACCAATGA CGATTTCTTC GAAATGATGA 61 AACGCTCATA AATTTGTCTT ATGCCAAAAA CGCCACGTGT TTACGTGGCG TTTTGCTTTT 121 ATATCTGTAA TCTTAATGCC GCGCTGGCGA TGTTAGGAAA ATTCCTGGAA TTTGCTGGCA 181 TGTTATGCAA TTTGCATATC AAATGGTTAA TTTTTGCACA GGACTGGTGG GTTTGGAACG 241 GACTTTCCCT TCTGAATAAA GGTCTTCGTG GTTATACTTC TGCTAATAAT TTTCTCTGAG 301 AGCATGCATT GTGAATTTAC TGACAGTGAG TACTGATCTC ATCAGGGATC TGCCACGCAC 361 AAAACTACGG TAGCAAATGT TCGGATTAGG ACACAACTCA AAAGAAATCT CGATGAGTCA 421 TATTGGTACT AAATTCATTC TTGCTGAAAA ATTTACCTTC GATCCCCTAA GCAATACTCT 481 GATTGACAAA GAAGATAGTG AAGAGATCAT TCGATTAGGC AGCAACGAAA GCCGTATCCT 541 TTGGCTGCTG GCCCAACGTC CAAACGAGGT AATTTCTCGC AATGATTTGC ATGACTTTGT 601 TTGGCGAGAG CAAGGTTTTG AAGTCGATGA TTCCAGCTTA ACCCAAGCCA TTTCGACTCT 661 GCGCAAAATG CTCAAAGATT CGACAAAGTC CCCACAATAC GTCAAAACGG TTCCGAAGCG 721 CGGTTACCAA TTGATCGCCC GAGTGGAAAC GGTTGAAGAA GAGATGGCTC GCGAAAACGA 781 AGCTGCTCAT GACATCTCTC AGCCGGAGTC CGTCAATGAA TACGCAGAAT CAAGCAGTGT 841 GCCTTCATCT GCTACCGTAG TGAACACACC GCAGCCAGCC AATGTCGTGG CGAATAAATC 901 GGCTCCAAAC TTGGGGAATC GACTGTTTAT TCTGATAGCG GTCTTACTTC CCCTCGCAGT 961 ATTACTGCTC ACTAACCCAA GCCAATCCAG CTTTAAACCC CTAACGGGAT CtGCTAGCGG 1021 AAGCGGATCG ACCAAGAAAA AGCCATTAAC ACAAGAGCAG CTTGAGGACG CACGTCGCCT 1081 TAAAGCAATT TATGAAAAAA AGAAAAATGA ACTTGGCTTA TCCCAGGAAT CTGTCGCAGA 1141 CAAGATGGGG ATGGGGCAGT CAGGCGTTGG TGCTTTATTT AATGGCATCA ATGCATTAAA 1201 TGCTTATAAC GCCGCATTGC TTGCAAAAAT TCTCAAAGTT AGCGTTGAAG AATTTAGCCC 1261 TTCAATCGCC AGAGAAATCT ACGAGATGTA TGAAGCGGTT AGTATGCAGC CGTCACTTAG 1321 AAGTGAGTAT GAGTACCCTG TTTTTTCTCA TGTTCAGGCA GGGATGTTCT CACCTGAGCT 1381 TAGAACCTTT ACCAAAGGTG ATGCGGAGAG ATGGGTAAGC ACAACCAAAA AAGCCAGTGA 1441 TTCTGCATTC TGGCTTGAGG TTGAAGGTAA TTCCATGACC GCACCAACAG GCTCCAAGCC 1501 AAGCTTTCCT GACGGAATGT TAATTCTCGT TGACCCTGAG CAGGCTGTTG AGCCAGGTGA 1561 TTTCTGCATA GCCAGACTTG GGGGTGATGA GTTTACCTTC AAGAAACTGA TCAGGGATAG 1621 CGGTCAGGTG TTTTTACAAC CACTAAACCC ACAGTACCCA ATGATCCCAT GCAATGAGAG 1681 TTGTTCCGTT GTGGGGAAAG TTATCGCTAG TCAGTGGCCT GAAGAGACGT TTGGCGCTGC 1741 AAACGACGAA AACTACGCTT TAGTAGCTTA AGGATCCtaa CTCGAcgtgc aggcttcctc 1801 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 1861 ggcggtaatC AATTCGACCC AGCTTTCTTG TACAAAGTTG GCATTATAAA AAATAATTGC 1921 TCATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT 1981 CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC 2041 TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT ATCATCATGC 2101 CTCATCTAGA CCAGCCAGGA CAGAAATGCC TCGACTTCGC TGCTGCCCAA GGTTGCCGGG 2161 TGACGCACAC CGTGGAAACG GATGAAGGCA CGAACCCAGT GGACATAAGC CTGTTCGGTT 2221 CGTAAGCTGT AATGCAAGTA GCGTATGCGC TCACGCAACT GGTCCAGAAC CTTGACCGAA 2281 CGCAGCGGTG GTAACGGCGC AGTGGCGGTT TTCATGGCTT GTTATGACTG TTTTTTTGGG 2341 GTACAGTCTA TGCCTCGGGC ATCCAAGCAG CAAGCGCGTT ACGCCGTGGG TCGATGTTTG 2401 ATGTTATGGA GCAGCAACGA TGTTACGCAG CAGGGCAGTC GCCCTAAAAC AAAGTTAAAC 2461 ATCATGAGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC TATCAGAGGT AGTTGGCGTC 2521 ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT TGTACGGCTC CGCAGTGGAT 2581 GGCGGCCTGA AGCCACACAG TGATATTGAT TTGCTGGTTA CGGTGACCGT AAGGCTTGAT 2641 GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA CTTCGGCTTC CCCTGGAGAG 2701 AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC ACGACGACAT CATTCCGTGG 2761 CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC AGCGCAATGA CATTCTTGCA 2821 GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA TCTTGCTGAC AAAAGCAAGA 2881 GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC TCTTTGATCC GGTTCCTGAA 2941 CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT GGAACTCGCC GCCCGACTGG 3001 GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA TTTGGTACAG CGCAGTAACC 3061 GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA TGGAGCGCCT GCCGGCCCAG 3121 TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG GACAAGAAGA AGATCGCTTG 3181 GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCACTACG TGAAAGGCGA GATCACCAAG 3241 GTAGTCGGCA AATAACCCTC GAGCCACCCA TGACCAAAAT CCCTTAACGT GAGTTACGCG 3301 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 3361 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 3421 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 3481 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 3541 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 3601 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 3661 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 3721 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 3781 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 3841 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 3901 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 3961 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 4021 TCTGTGGATA ACCGTcctag gTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA 4081 ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 4141 TTTATAATGC CAACTTTGTA CAAAAAAGCA GGCTCCGAAT TGgtatcacg aggcagaatt 4201 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctgGAATTCA TGA //