LOCUS Exported 4880 bp ds-DNA circular SYN 16-FEB-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS pJT176 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4880) AUTHORS . TITLE Direct Submission JOURNAL Exported Sunday, Feb 16, 2014 from SnapGene Viewer 2.2.2 http://www.snapgene.com COMMENT LOCUS pETDuet-1 5420 bp DNA circular 22-AUG-2002 DEFINITION pETDUET-1 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|275404061| COMMENT VNTDBDATE|275677264| COMMENT VNTNAME|pETDuet-1| COMMENT VNTAUTHORNAME|Bob Novy| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers source 1..4880 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 16 /note="G" misc_feature 17..56 /note="U4" misc_feature 57..58 /note="CG" promoter 92..108 /note="T7 promoter" protein_bind 113..133 /note="lacO" RBS 166..171 /note="RBS-1" misc_feature 179..181 /note="START" misc_binding 203..220 /note="His-Tag" misc_feature 236..238 /note="STOP" terminator 290..337 /note="T7 Terminator" misc_feature 358 /note="G" misc_feature 359..398 /note="U5" misc_feature 399..400 /note="CG" misc_feature 413 /note="G" misc_feature 414..453 /note="UX" misc_feature 454..455 /note="CG" CDS complement(809..1669) /note="Ap" /note="B-lactamase gene" -10_signal complement(1711..1717) /note="-10 Ap" -35_signal complement(1735..1740) /note="-35 Ap" rep_origin 2430 /direction=RIGHT /note="ColE1, pBR322 ori" CDS complement(2861..3052) /note="ROP" CDS complement(3624..4706) /note="lacI" /note="lac repressor gene" ORIGIN 1 aattgctctt cttaagctga cctcctgcca gcaatagtaa gacaacacgc aaagtccgcc 61 tgcattagga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga 121 gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat 181 gggcagatct actagtagca gccatcacca tcatcaccac agcggatccg ctagctgact 241 gcagagaagc ttgcggccgc ctcgagtcgc ctaggccgct gagcaataac tagcataacc 301 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaacctcag gaccggtgga 361 gccaactccc tttacaacct cactcaagtc cgttagagcg cgcgcggtct tcgccaggat 421 acatagatta ccacaactcc gagcccttcc acccgccggc gagacccacg tagtgggcca 481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 541 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 661 gcgaatttta acaaaatatt aacgtttaca atttctggcg gcacgatggc atgagattat 721 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 781 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 841 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 901 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 961 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 1021 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 1081 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 1141 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 1201 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 1261 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 1321 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 1381 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 1441 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 1501 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 1561 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 1621 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 1681 ttcaatcatg attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 1741 tgtatttaga aaaataaaca aataggtcat gaccaaaatc ccttaacgtg agttttcgtt 1801 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1861 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1921 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1981 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 2041 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 2101 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 2161 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 2221 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 2281 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 2341 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 2401 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 2461 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 2521 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 2581 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac 2641 gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat 2701 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 2761 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 2821 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 2881 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac 2941 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct 3001 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct 3061 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc 3121 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac 3181 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct 3241 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc 3301 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac 3361 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg 3421 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc 3481 gggtcctcaa cgacaggagc acgatcatgc tagtcatgcc ccgcgcccac cggaaggagc 3541 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct 3601 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 3661 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg 3721 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc 3781 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg 3841 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc 3901 gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc 3961 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg 4021 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt 4081 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt 4141 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc 4201 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca 4261 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca 4321 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc 4381 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt 4441 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg 4501 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg 4561 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg 4621 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg 4681 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc 4741 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc 4801 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac 4861 cgccgccgca aggaatggtg //