LOCUS pRS412 6042 bp DNA circular 30-MAR-2006 DEFINITION Cloning vector pRS412 with CEN/ARS ADE2 marker, complete sequence. KEYWORDS ATCC 87478; ADE2; CEN6/ARS4; lacZ'; S. cerevisiae; Updated 12/23/97 MB3. SOURCE ORGANISM COMMENT This file is best viewed with a free Vector NTI Viewer: COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|406292692| COMMENT LSOWNER| COMMENT VNTNAME|pRS412| COMMENT VNTAUTHORNAME|MINGTANG CHEN| COMMENT VNTREPLTYPE|Phagemid COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Yeast COMMENT VNTUDF|Freezer Position|1|126:27| FEATURES Location/Qualifiers CDS 575..2290 /vntifkey="4" /label=ADE2\CDS rep_origin complement(2495..2951) /vntifkey="33" /label=f1\ori promoter 3121..3140 /vntifkey="30" /label=T7\promoter promoter complement(3267..3286) /vntifkey="30" /label=T3\promoter CDS complement(2952..3311) /vntifkey="4" /label=LACZ' promoter complement(3356..3384) /vntifkey="30" /label=lac\promoter rep_origin 3710 /vntifkey="33" /label=pMB1\ori CDS complement(4468..5328) /vntifkey="4" /label=ampR misc_feature 5470..5586 /vntifkey="21" /label=CEN6 rep_origin 5587..5988 /vntifkey="33" /label=ARS4 misc_feature 187..2442 /vntifkey="21" /label=ADE2\Selection\Marker BASE COUNT 1749 a 1295 c 1354 g 1644 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatagatc tgaattaatt cttgaataat acataacttt tcttaaaaga atcaaagaca 241 gataaaattt aagagatatt aaatattagt gagaagccga gaattttgta acaccaacat 301 aacactgaca tctttaacaa cttttaatta tgatacattt cttacgtcat gattgattat 361 tacagctatg ctgacaaatg actcttgttg catggctacg aaccgggtaa tactaagtga 421 ttgactcttg ctgacctttt attaagaact aaatggacaa tattatggag catttcatgt 481 ataaattggt gcgtaaaatc gttggatctc tcttctaagt acatcctact ataacaatca 541 agaaaaacaa gaaaatcgga caaaacaatc aagtatggat tctagaacag ttggtatatt 601 aggaggggga caattgggac gtatgattgt tgaggcagca aacaggctca acattaagac 661 ggtaatacta gatgctgaaa attctcctgc caaacaaata agcaactcca atgaccacgt 721 taatggctcc ttttccaatc ctcttgatat cgaaaaacta gctgaaaaat gtgatgtgct 781 aacgattgag attgagcatg ttgatgttcc tacactaaag aatcttcaag taaaacatcc 841 caaattaaaa atttaccctt ctccagaaac aatcagattg atacaagaca aatatattca 901 aaaagagcat ttaatcaaaa atggtatagc agttacccaa agtgttcctg tggaacaagc 961 cagtgagacg tccctattga atgttggaag agatttgggt tttccattcg tcttgaagtc 1021 gaggactttg gcatacgatg gaagaggtaa cttcgttgta aagaataagg aaatgattcc 1081 ggaagctttg gaagtactga aggatcgtcc tttgtacgcc gaaaaatggg caccatttac 1141 taaagaatta gcagtcatga ttgtgaggtc tgttaacggt ttagtgtttt cttacccaat 1201 tgtagagact atccacaagg acaatatttg tgacttatgt tatgcgcctg ctagagttcc 1261 ggactccgtt caacttaagg cgaagttgtt ggcagaaaat gcaatcaaat cttttcccgg 1321 ttgtggtata tttggtgtgg aaatgttcta tttagaaaca ggggaattgc ttattaacga 1381 aattgcccca aggcctcaca actctggaca ttataccatt gatgcttgcg tcacttctca 1441 atttgaagct catttgagat caatattgga tttgccaatg ccaaagaatt tcacatcttt 1501 ctccaccatt acaacgaacg ccattatgct aaatgttctt ggagacaaac atacaaaaga 1561 taaagagcta gaaacttgcg aaagagcatt ggcgactcca ggttcctcag tgtacttata 1621 tggaaaagag tctagaccta acagaaaagt aggtcacata aatattattg cctccagtat 1681 ggcggaatgt gaacaaaggc tgaactacat tacaggtaga actgatattc caatcaaaat 1741 ctctgtcgct caaaagttgg acttggaagc aatggtcaaa ccattggttg gaatcatcat 1801 gggatcagac tctgacttgc cggtaatgtc tgccgcatgt gcggttttaa aagattttgg 1861 cgttccattt gaagtgacaa tagtctctgc tcatagaact ccacatagga tgtcagcata 1921 tgctatttcc gcaagcaagc gtggaattaa aacaattatc gctggagctg gtggggctgc 1981 tcacttgcca ggtatggtgg ctgcaatgac accacttcct gtcatcggtg tgcccgtaaa 2041 aggttcttgt ctagatggag tagattcttt acattcaatt gtgcaaatgc ctagaggtgt 2101 tccagtagct accgtcgcta ttaataatag tacgaacgct gcgctgttgg ctgtcagact 2161 gcttggcgct tatgattcaa gttatacaac gaaaatggaa cagtttttat taaagcaaga 2221 agaagaagtt cttgtcaaag cacaaaagtt agaaactgtc ggttacgaag cttatctaga 2281 aaacaagtaa tatataagtt tattgatata cttgtacagc aaataattat aaaatgatat 2341 acctattttt taggctttgt tatgattaca tcaaatgtgg acttcataca tagaaatcaa 2401 cgcttacagg tgtccttttt taagaatttc atacataaga tctatgcggt gtgaaatacc 2461 gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta 2521 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 2581 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 2641 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 2701 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 2761 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 2821 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 2881 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 2941 cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 3001 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 3061 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg 3121 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc 3181 gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag agcggccgcc 3241 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta 3301 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 3361 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 3421 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 3481 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg 5461 gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat 5521 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag 5581 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct 5641 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc 5701 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct 5761 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta 5821 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat 5881 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga 5941 tacgaggcgc gtgtaagtta caggcaagcg atccgtccta agaaaccatt attatcatga 6001 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc //