LOCUS Exported 4370 bp ds-DNA circular SYN 14-APR-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS pDestET (renamed) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4370) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Apr 14, 2014 from SnapGene Viewer 2.2.2 http://www.snapgene.com COMMENT LOCUS pETDuet-1 5420 bp DNA circular 22-AUG-2002 DEFINITION pETDUET-1 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|275404061| COMMENT VNTDBDATE|275677264| COMMENT VNTNAME|pETDuet-1| COMMENT VNTAUTHORNAME|Bob Novy| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers source 1..4370 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1 /note="SphI" misc_feature 13 /note="Untitled" misc_feature 14..53 /note="New L1" misc_feature 54..55 /note="Untitled" misc_feature 88 /note="lMauBI" misc_feature 89..128 /note="New LX" misc_feature 129..130 /note="Untitled" primer_bind 157..163 /note="T7 Terminator Primer #69337-3" rep_origin 164..173 /direction=RIGHT /note="f1 ori" CDS complement(295..1155) /note="Ap" /note="B-lactamase gene" -10_signal complement(1197..1203) /note="-10 Ap" -35_signal complement(1221..1226) /note="-35 Ap" rep_origin 1916 /direction=RIGHT /note="ColE1, pBR322 ori" CDS complement(2347..2538) /note="ROP" CDS complement(3110..4192) /note="lacI" /note="lac repressor gene" misc_feature 4301..4311 /note="Untitled" misc_feature 4366..4370 /note="SphI" ORIGIN 1 cgctcttctt aagcattact cgcatccatt ctcaggctgt ctcgtctcgt ctccgcgcgc 61 ggtcttcctg gagatgatca aacctaggcc aggatacata gattaccaca actccgagcc 121 cttccacccg ccggcgagac ctctaggctg ctgccaccgc tgaattaacg tttacaattt 181 ctggcggcac gatggcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 601 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 901 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 1021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 1081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 1141 atgttgaata ctcatactct tcctttttca atcatgattg aagcatttat cagggttatt 1201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggtcatgacc 1261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 1321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 1381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 1441 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 1501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 1561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 1621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 1681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 1741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 1801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 1861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 1921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 1981 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 2041 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 2101 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt 2161 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 2221 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 2281 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 2341 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc 2401 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt 2461 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt 2521 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat 2581 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg 2641 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa 2701 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag 2761 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt 2821 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga 2881 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc 2941 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgctagt 3001 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg 3061 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc 3121 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 3181 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca 3241 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt 3301 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt 3361 cttcggtatc gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc ccggactcgg 3421 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa 3481 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc 3541 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca 3601 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac 3661 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac 3721 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag 3781 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc 3841 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca 3901 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt 3961 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc 4021 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca 4081 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct 4141 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca 4201 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt 4261 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc 4321 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg //