1. Get Pellet	
	culture 1.5 mL overnight
	centrifuge for 5 mins at 14,000 RPM
	discard supernatant

2. Wash Pellet						Solution 1			
	Resuspend pellet in 400 uL ice-cold acetone	50 mM glucose
	centrifuge for 5 mins at 14,000 RPM		25 mM Tris/HCL
	discard supernatant 				10 mM EDTA
							20 mg/mL lysozyme (Fresh)
3. Lyse cells						100 mg/mL RNase	  (Fresh)		
	Resuspend in 200 uL of Solution 1			
	vortex						
	incubate at 37 degrees C for 15 mins		Solution 2
							.2 M NaOH				
4. Denature Protiens					2% SDS  (Fresh)
	add 300 uL of Solution 2
	mix by inversion 
	incubate at room temperature for 3 mins		Solution 3
							1.2 M Tris/HCL
5. Buffer Solution					2 M NaCl
	Add 170 uL of ice-cold Solution 3
	mix by inversion
	incubate at room temp for 3 mins		PCA
							25 parts Phenol
6. Separate Proteins					24 parts Choroform
	add 500 uL phenol				1 part amyl-alchohol
	mix by inversion				by volume
	centrifuge 3 mins at 14,000 RPM
	collect upper phase
							TE
7. Separate Genomic DNA					10 mM Tris/HCL
	add 600 uL of PCA 				1 mM EDTA
	mix by inversion
	centrifuge 3 mins at 14,000 RPM
	collect upper phase

8. Condense plasmid DNA
	add 600 uL isopropyl-alchohol
	mix by inversion
	put in freezer for 15 minutes
	centrifuge for 10 mins at 14,000 RPM
	discard supernatant

9. Wash DNA pellet
 	gently add 1 mL of 70% ethanol
	let sit for one min
	discard liquid and dry pellet

10. Resuspend pellet
	disolve pellet in 20 uL of TE (pH 8)