Tk:wanted parts


 * Expression biobrick plasmids
 * T7 promoter
 * T3
 * SP6
 * T7 RNA polymerase containing strains
 * also T3, SP6 What are the patent issues on these?


 * xre protein, promoters (subtilis)


 * antibiotic cassettes
 * Apramycin
 * Trimethoprim


 * Transposase genes


 * R6 origin plasmids


 * chomosomal integration fragments
 * choice of locations on the chromosome


 * cell killing enzymes
 * VSVG


 * protein export tags


 * protein cleavage domains


 * protein purification tags, antibody epitopes
 * FLAG
 * DYKDDDDK, pmid 8770418
 * FLAG Epitope description
 * c-MYC
 * EQKLISEEDL
 * HA
 * YPYDVPDYA
 * 6HIS
 * HHHHHH
 * Strep
 * AWRHPQFGG
 * Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
 * GST (n-terminal)
 * Chitin binding
 * BCCP biotin carboxyl carrier protein
 * PMID: 14736427
 * PMID: 10211839
 * PMID: 10470036
 * VSVG
 * Thrombin cleavage domain
 * LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
 * Calmodulin tag
 * S-tag
 * GFP protein tags


 * protein solubility
 * maltose binding protein
 * Thioredoxin


 * protein splicing domains (inteins)


 * surface display proteins
 * LPP/OmpA
 * Neisseria IgA1


 * New fluorescent proteins
 * EBFP2 blue
 * new red monomers


 * luminescent reporters
 * renilla
 * photuris
 * bacterial (Photorhabdus)
 * dual reporter renilla/photuris being done with John Cumbers (Brown)
 * dual reporter red/green beetle luciferase dual color


 * enzymatic reporters
 * HRP protein
 * lacZ
 * Alkaline phosphatase


 * Zinc finger domains


 * Repair enzymes
 * UDG
 * mutS, mutC


 * RNA sensor domains for all small molecules


 * negative selection markers
 * sacB
 * pheS mutated


 * toxic proteins
 * ccdB and similar


 * Mesoplasma florum genes
 * recT, recE
 * GFP