User:Fermenter User/Notebook/BROM C8

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM C8

Day 0 (Aug 23 Mon):
      8/23/2010 (12:00) Bring media to fermenter room            8/23/2010 (12:00) Assemble bioreactor 8/23/2010 (12:00) Add 10 ml of Antifoam/fermenter 8/23/2010 (13:55) Autoclave (Liq 45 min) 8/23/2010 (15:30) Cooling of bioreactor (Temp setup) 1 L/min air, 300 rpm 8/23/2010 (15:46) Start Fermentation Software for Fermenter #6 8/23/2010 (15:48) Start Fermentation Software for Fermenter #7 8/23/2010 (16:00) Connect Acid (250 ml) and Base (400 ml) Start pH control 8/23/2010 (16:40) Calibrate dO2 probe with 300 rpm, Air purge for 30 min 8/23/2010 (16:40) Set dO2

      8/23/2010 (17:00) Inoculation: inoculation volume (300 ml) Fermentation 0:00 F6: Cell weight (?? mg), Activity (15.7%, x100), Activity (42.1%, x1000) F7: Cell weight (?? mg), Activity (15.3%, x100), Activity (37.9%, x1000)

Day 1 (Aug 24 Tue):
           8/24/2010 (9:00) Increase rpm to 550       8/24/2010 (10:00) Sampling  Fermentation 19:00 F6: Cell weight (?? mg), Activity (1.56%, x100), Activity (15.4%, x1000) F7: Cell weight (?? mg), Activity (0.99%, x100), Activity (2.49%, x1000)

           8/24/2010 (11:30) Fermentation 21:00 Harvest Fermentation F6: Acid (220 ml), Base (200 ml) F7: Acid (210 ml), Base (250 ml)


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