User:Karmella Haynes/Notebook/miR Trigger/2011/01/24

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

01/24/11

 * &#x2713; Transfections: check RFP expression of Repressor-transfectants
 * &#x2713; Cultures: thaw JDS33-47; mine 4/2010, and one from Joe 2/2010; use 20%FBS medium

RFP expression > Saw same amount of RFP expression from all 6 plasmids > RFP from reverse constructs shows that polyA BioBrick works (or at least does not stop expression) > Continue with miR circuit consruction by placing CFP-Target genes in front of Repressors


 * }