Berglund:Gel Dryer Method

Materials
Stain: 0.25g Coomassie Blue R-250 100ml ethanol 100ml water stir until dye has dissolved (about 1 hr), add: 25ml glacial acetic acid 25ml water Destain: 400ml ethanol 100ml glacial acetic acid 500ml water

Methods

 * 1) take gel apart in water
 * 2) cut off top of gel (stacking part of gel and wells)
 * 3) rinse in water a couple of times to get rid of SDS
 * 4) shake in 25ml of destain for 5-10 min then pour off destain
 * 5) add 50ml of stain (can be reused)
 * 6) stain for 30min- 1hr (stain can be heated briefly in microwave to hasten staining process)
 * 7) pour off stain
 * 8) rinse with water a couple of times
 * 9) add destain (25-50ml)
 * 10) stop destain with water when bands are quite visible
 * 11) put on whatman paper and dry in gel dryer for about 15 min