User:Mark X. Ling/Notebook/Micb 323/2009/02/03

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Part 1: Plasmid Isolation

 * cells were isolated from: Lab 3 part 1, white and blue colony, and part 2A colony(plate 2A3)

Part2:Restriction Digest of plasmid

 * no change same as in lab manual.

Section 1: Normal restriction digest

 * time incubated: 2:09
 * temperature incubated:37 oC

DNA content: **measurement done on Part 1 white colony. ligated. *A260nm:0.200 **more than 0.15 so may not be accurate. *A280nm:0.107 *A260/A280:1.89 *concentration DNA: 2000ug DNA/mL

Section 2: Modified Restriction digest
EcoR1:

NaCl:


 * digests incubated at: 2:58
 * incubated at:37/38.2 oC
 * MISTAKE: used 4ug of DNA instead of 0.2ug

Part 3: Insect Tissue culture infected with Baculovirus

 * Time incubated on room temperature:325
 * room temperature:22 oC
 * harvest schedule=> Tue:Eric => Wed:Mark => Thu:Crystal => Fri: Mark => Mon:Sunny

Post Lab comments

 * Miscalculation DNA was done.
 * had too much DNA in the RE enzyme modification
 * all samples are of extreme low. Will not be useful in assessment of the need of each components.
 * DNA isolation went smoothly
 * seemed to have good amount BUT the A260nm was higher than 0.200, so there may be RNA contamination.


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