Griffitts:PCR clean-up

Materials

 * Buffer PB (in Qiagen kit)
 * Buffer PE (in Qiagen kit)
 * Buffer EB (in Qiagen kit)
 * QiaPrep spin column (1 per PCR reaction)
 * 1.5 mL microcentrifuge tube (1 per PCR reaction)

Procedure

 * Label spin columns and microcentrifuge tubes with the names of the appropriate PCR reactions
 * Using a P-200, add 200 μL buffer PB to each 40-μL PCR and mix well by gently pipetting up and down
 * Transfer the mixture to a QiaPrep spin column
 * Centrifuge at full speed for 30 seconds
 * Dump and tap
 * Add 700 μL buffer PE to each column
 * Centrifuge at full speed for 30 seconds
 * Dump and tap
 * Centrifuge for an additional 5 minutes at full speed
 * Move the column to a 1.5 mL microfuge tube
 * Add 50 μL buffer EB (to elute)
 * Drip this directly onto the filter disk but don't touch it with the pipette tip
 * Wait 2 minutes
 * Centrifuge at full speed for 1 minute
 * Point the lids of the microcentrifuge tubes in a clockwise direction, otherwise they'll break off