User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/09/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Virgin control and experimental flies
 * Collect this week, set up Friday
 * Dissect Tdt-Myb
 * Dissected 13 testis.
 * Fixed 30 min in 4% formaldehyde PBS solution.
 * Washed 3x5' in PBST
 * Blocked 1 hr with 2% NGS solution.
 * Heather changed solution and incubated with 1:1000 rabbit anti-RFP at 4°C O/N.


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