Keck Subbasement:Schedule

Week 1
The goal of week 1 is to do a basic experiment so that you will become familiar with the lab and standard molecular biology procedures. We will be exchanging yellow fluorescent protein (YFP) for cyan fluorescent protein (CFP) on a plasmid and then quantifying the results with microscopy.

Monday

 * Pick colonies and grow o/n
 * Vector NTI discussion of two plasmids
 * Design colony PCR/sequencing primers

Tuesday

 * Miniprep plasmid pNNS2-ySMc
 * Amplify CFP
 * Check amplification on a gel
 * Miniprep plasmid pZE12-YFP
 * Digest vector (pZE12-YFP) and insert (YFP PCR product)

Wednesday

 * Gel extraction (vector)
 * PCR purification (insert)
 * Ligation
 * Transformation into competent cells (PM)

Thursday

 * Colony PCR to check for correct insertion
 * PCR purify and prepare product for sequencing
 * Online databases??

Friday

 * Send sequencing
 * Make agarose pads
 * Microscopy