Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/8.1.08 OD Log

=8.1.08 OD Log=

(1/20 dilution of culture)

I diluted 50 &mu;l of culture in 950 &mu;l LB and pipetted up and down. Multiply each value by 20 to get actual OD. The P5 and P6 cultures are already way overgrown. I'm diluting them back 1/10 (P1 1/2) and growing up for 20-25 minutes.

1/20 Dilutions