IGEM:MIT/2007/Notebook/2007-7-3

Agenda for July 3

 * Call Geneart
 * Results of Alex/Diti PCR?
 * For metals: RBS and promoter? (clarify!)
 * Barry's promoter -- obtainment?
 * Discuss results of Monday's 2PM meeting
 * Set up meeting with Drew/Tom/grads?

Wet Work

 * All plates look like sludge
 * Not sure why
 * Could be need to use LB+KAN for recovery step
 * Plating with 1000 µl instead of 100 µl

Make LB+KAN agar plates

 * 1) Heat LB+KAN agar plates
 * 2) *Heat LB + Agar 1.2% for 10 minutes at 50% power
 * 3) *Wait until cool to touch and put in 960(???) µL Kanamycin to make 10 µg/ml concentration
 * 4) *Pour into labeled plates

Incubate 5 colonies of DB3.1 strain with pSB3k3 plasmid (with Kan+)

 * Started at 6pm (spinning in hot room)
 * +18 hours = done 12pm tomorrow

Incubated plates of DH5a

 * Francois kindly gave us two plates of untransformed DH5a because our own line is probably compromised
 * Incubated plates at 37°C overnight