User:Karmella Haynes/Notebook/BioBrick cloning/2011/06/30

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06/30/11

 * &#x2713; Minipreps: SP1A (2), SP1B (2), KAH199 (2), KAH200 (2)
 * &#x2713; Site directed mutagenesis: continue from 6/29/11
 * &#x2713; Sequencing order: miR sensors set #1 (whole plate failed last time, time try smaller order w/ strip tubes)

Minipreps > Check SP1A, SP1B, KAH199 with E/P digests > Check KAH200 with E/S digest

--> KAH200 failed. Try DPRE assembly later with fresh insert. Reassign this number to different construct. --> Keep 1-4 for sequence confirmation (tomorrow).

Sequencing --> Primer = DD122 (forward)


 * 1) 186MV8: good
 * 2) 184MV8: good
 * 3) 187MV8: good
 * 4) 186-196MV8: no priming
 * 5) 184-196MV8: "
 * 6) 187-196MV8: "
 * 7) 186-177MV8: "
 * 8) 184-177MV8: "
 * 9) 187-177MV8: "
 * 10) 186-198MV8: "
 * 11) 184-198MV8: "
 * 12) 187-198MV8: "

--> Arggghhh! What is happening to my plasmids?


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