Klapperich Lab:Notebook/Lab Meeting Notes/2009/06/09

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9 June 2009 Lab Meeting
Missing: Alex † Announcements Suggested groups... please edit. Teams 1. PCR= QQ, MCK, MM 2. Bacteria = JZ, JD 3. Flu = MM, JZ, HM, JC, BC 3. HDA? Sample Preparation = AG, HM? † Flu R01
 * attening: Hussam, Jaephil, Jane, MinCheol, Brendan, Jessie, QQ, Sonali, Me.
 * Jaephil gave small evap presentation to the group.
 * Check on your NEW LAB DUTIES WITH SONALI Then do them and check off when they are done each week.
 * Short small team meetings. 45 min -- SEE GOOGLE calendar for schedule
 * MicroTAS Abstracts accepted. (4) Papers due June 30th.
 * RNA extraction troubleshooting.
 * Try clean fabrication of chips and monoliths.
 * Try RNAseH wash through.
 * Absolute Quantification assays: TH this week.
 * FTIR/maybe next week.
 * Samples for Mass Spec.
 * A primers look good at this point. Van Elden 2001.
 * Jessie should learn SEM.

† Virus concentration (upstream from the assay.) † Coulter Flu Fraunhofer Project † SEPSIS Project †RNA project † COBRA paper 1: Evap with Sol Gel substrate. paper 2: Covap with PMA, Rhodamine, PS beads?...virus...? - Rhodamine experiments done - Signals are similar between with and without GNP - Rhodamine binds poorly to gold - try GNP-pMA next. R6G-silver NP. - Need to redefine hypothesis
 * QQ - HM - JZ - Chip Integration.
 * How to read? Color, sensitivity? Alignment. Check with JD.
 * Fraun folks coming to get trained next week. RNA isolation. Safety issues. English.
 * IBC for Fraun flu. waiting?
 * Brendan can come to Fraun Flu meetings if he wants....as primer design goes.
 * Accepted YEAH!
 * Call Natalia.
 * Test with sol-gel substrate today(2nd June)
 * delayed due to difficulty adjusting membrane thickness
 * Evap system (JD and JZ)
 * Let's get this to a point where we can publish and move on

† Valve Array † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant
 * Teddy will give training presentation(guide).
 * Paper revised.
 * Bonding ongoing with MIT folks
 * Meeting Wed 2-4pm.
 * Printed out paper for everyone. Go over the figures. Set the "story."
 * fabricated SU-8 mold with Herringbone grooves.
 * Brett took a photo of the device for the paper, but will repeat
 * Jason coated thin gold on the surface of the PDMS mold, and will take SEM images for the sieves. --->SEM down
 * draft paper2 for the submission to "Biophysical Journal" has been started.
 * Will repeat trapping experiments this Friday to obtain good quality images for the Fig. 2 in the paper.
 * IRB still in revision.

† PCR † RCA/HDA
 * QQ: PCR 2 paper draft.
 * CMK: PCR 1 draft
 * PEG-coating protocol developed - TROUBLESHOOTING.
 * on-chip experiment with blank chip without PEG,BSA. It works with expected lower efficiency
 * on-chip experiment with PEG-coated chip without PEG,BSA in reagent. It does not work, or the product is under the detection limitation of bioanalyzer. trouble-shooting...
 * QQ : write protocol for PEG graft.
 * QQ: Try changing heater and thermocouple.
 * QQ: Try running PCR after plasma treatment and water wash only.
 * QQ and MM: look back at flu PCR. Recall the repeatability. Decide course of action with Sonali. See what you can do to do "real" samples soon. One pot? Two steps necessary instead?
 * Getting solution back out is still an issue.
 * Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime)
 * Evaporation problems near edges. Maybe design change?
 * Teflon issue with the enzyme? Check into it.

† Silica Optimization (Lambda):
 * Get lower Bioan. Kit.
 * Get lower Bioan. Kit.


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