User:TheLarry/Notebook/Larrys Notebook/2009/11/15

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Generating Data
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Airy Disc Smear
I used this equation from yesterday $$\nu =2\pi \frac{NA}{\lambda}r_0$$ I changed the wavlength into pixels so i can work in that unit. I used the factor Andy gave me yesterday. Anyway using pixels the prefactor came out to be 2.71 1/pixels. This gave me a pretty nice looking Airy Function. So I think i am on the right path.

Now i need to figure out the max intensity. Which i think will be just fitting it by eye. I can't think of a better way to do this because it can change often--from bleaching, so it won't be mathematical like this smear was. I am pretty happy with this



So I can get started on trying to fit this intensity or i can start by throwing random fluorescent dots onto a rectangle that will symbolize the MT. '''Hey Koch, i can't remember how we decided to split up the microtubule can you chuck a comment here to refresh my memory? Thanks'''

There should be a good way to figure out this maximum intensity for the dots. I can make this monte carlo algorithm and then see how many of them overlap and then fit that to the image adjusting the intensity at the end. That sounds like the best idea.

The basic idea is that i break up the rectangle that is approximating the microtubule into x subsections. Then run through those subsections and roll the dice for each one. If it is lower than the concentration percentage i put a dot there if not i move on. I am not sure how thick the rectangle will be. I have the feeling that it will be more like a line. I am not sure how wide a microtubule is but if it is less than 180 nm (that is basically what makes up 1 pixel) it can be a line.


 * }