User:Anthony Salvagno/Notebook/Research/2010/03/29/Power Spectrum w/ Pranav

Prep
Half the prep is getting my notebook ready and this was tough. It took me a while to figure out the feeds of Evernote, but I got it. To access your RSS you need:
 * 1) share your notebook
 * 2) go to the public notebook
 * 3) find the feed icon and then copy the feed url
 * 4) paste that in friendfeed or wherever you want

Ok here is what I need to do:
 * flow beads. What I did last time seemed to work nicely as far as limiting stuck beads.  I flowed BGB first and then flowed beads diluted in water.
 * determine trapping quality
 * run software
 * view beads
 * acquire power spectrum

For Tomorrow
Koch thinks we can go ahead and try unzipping tomorrow. I will write up a page in the morning and get to work, but here are some initial thoughts on what I need to get done:
 * 3 experimental samples - I will make 2 samples with double chambers using the double stick method.
 * just beads
 * dsDNA tethers 1.1kb
 * tpBR
 * I would like to analyze the beads only just to see if I have any better "results" than I did today
 * dsDNA tethers would be nice to get some stretching data
 * obviously tpBR is what we are ultimately trying for. I will do the just beads with the tpBR and do the beads like i'm doing the control (no DNA) but I won't wash after and I'll use diluted beads.