IGEM:Harvard/2010/General Protocols

=Synthetic Biology=
 * How Do BioBricks Work?
 * Strategy for Making Parts
 * PCR
 * Oligonucleotide Inserts
 * Site-Directed Mutagenesis
 * Restriction Digest
 * DNA Gel Electrophoresis and Fragment Purification
 * Measuring DNA Concentration Using a Nanodrop Spectrophotometer
 * Ligation
 * Quick & Dirty Digest & Ligation
 * Bacterial Transformation
 * Plasmid Verification
 * Qiagen RNeasy Plant Mini Kit
 * iScript cDNA Synthesis

=Microbiology=
 * Growing E. coli
 * Growing Agrobacterium
 * Making Electro-Competent Agrobacterium
 * Electroporating Agrobacterium

=Plants=
 * Growing Arabidopsis
 * Arabidopsis transformation via Flower Dipping