User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/18

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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HCO/LCO Adenita Culture Re-Amp
For failed sequencing samples 9, 11, 13, 14, 15, 41, 46, A, CCA3

Load 2.0ul Sample



HCO/LCO Adenita Culture Re-Amp PCR Purification with QIAquick
For failed sequencing samples 9, 11, 13, 14, 15, 41, 46, A, CCA3

http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2


 * see page 19.
 * 18ul PCR product left for cleaning. Resuspended in 50ul EB buffer.

NanoDrop results

HCO/LCO Adenita Culture Cycle Sequencing
For failed sequencing samples 9, 11, 13, 14, 15, 41, 46, A, CCA3


 * Seq Center cleaned and load onto genetic analyzer.