Knight:NuPAGE electrophoresis/Slow staining

Overview
A slow protocol for visualizing bands on a polyacrylamide gel via coomassie-like staining.

Materials

 * Deionized water
 * Invitrogen SimplyBlue SafeStain
 * Plastic tray
 * Use lid of a 1000&mu;L pipette tip box.
 * Orbital shaker
 * Kimwipes

Procedure

 * 1) Add 100mL deionized water in staining tray.
 * 2) Shake staining tray for 5 min on orbital shaker at room temperature.
 * 3) Discard water.
 * 4) Repeat steps 1-3 two more times.
 * 5) Add 20mL SimplyBlue SafeStain (enough to just cover the gel).
 * 6) Shake staining tray for 1 hr on orbital shaker at room temperature.
 * 7) *Overnight is better for improved sensitivity.
 * 8) Add 100mL deionized water.
 * 9) Shake staining tray for 1 hr or more on orbital shaker at room temperature.
 * 10) *If the gel has been stained overnight, let it destain for a few hours.