IGEM:UNAM/2009/Notebook/IGEM project/2011/06/08

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Ligation of Blunting

 * 1. Put 24 μl of destilled water to dilute DNA.
 * 2. Put 2 μl of Bluntig DNA.
 * 3. Put 3μl of Buffer T4 lis 10X.
 * 4. Put 1 μl of T4 DNA ligase.
 * 5. Put in the Centrifugate for 20 seconds to 10 mil rpm.
 * 6. Put to 25°C for 10 hours.

We did two tubes of DNA blunting with the last method.