IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/12/Zhangs

Determine the concentration of 1-5J plasmid by spectrophotometer. 1-5J② 7.55ng/μL×50=377.5ng/μL 1-5J③ 2.53ng/μL×50=126.5ng/μL

Double digestion for 1-5J+CI+1-23L insert again