IGEM:MIT/2007/Notebook/2007-7-8

Part(Plate #/ Pick#/ Nanodrop)
B0034 #1 Plate 2/ Part 2/ 84.4 ng/µl B0034 #2 F2620 #1 F2620 #2 E1010 #1 E1010 #2
 * 3/ 1/ 84.9 ng/µl
 * 3/ 2/ 134.5 ng/µl
 * 2/ 2/ 113.6 ng/µl
 * 1/ 2/ 162.8 ng/µl
 * 2/ 1/ 121.5 ng/µl

F2620
8.8 µl   F2620 0.5 µl   BSA 5 µl     NEB2 Buffer 2 µl     EcoRI 2 µl     SpeI 31.7 µl  H20

B0034
11.8 µl  B0034 0.5 µl   BSA 5 µl     NEB3 Buffer 2 µl     XbaI 2 µl     PstI 28.7 µl  H20

pSB3k3
38.2 µl  pSB3k3 0.5 µl   BSA 5 µl     NEB2 Buffer 2 µl     EcoRI 2 µl     SpeI 2.3 µl   H20


 * Add Enzymes Last