Restriction digest/Buffers

1X NEBuffer 1

 * 10 mM Bis-Tris-Propane-HCl
 * 10 mM MgCl2
 * 1 mM Dithiothreitol
 * pH 7.0 @ 25&deg;C

1X NEBuffer 2

 * 50 mM NaCl
 * 10 mM Tris-HCl
 * 10 mM MgCl2
 * 1 mM Dithiothreitol
 * pH 7.9 @ 25&deg;C

1X NEBuffer 3

 * 100 mM NaCl
 * 50 mM Tris-HCl
 * 10 mM MgCl2
 * 1 mM Dithiothreitol
 * pH 7.9 @ 25&deg;C

1X NEBuffer 4

 * 50 mM potassium acetate
 * 20 mM Tris-acetate
 * 10 mM Magnesium Acetate
 * 1 mM Dithiothreitol
 * pH 7.9 @ 25&deg;C