User:Karmella Haynes/Notebook/BioBrick cloning/2010/05/28

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

05/28/10

 * &#x2713; Minipreps: MV1 (2), MV7 (2) (no methylation)

Minipreps > Check with BsaBI/ BstBI digests (methylation sensitive) > Use old preps from DH5α as a control

--> 60°C (BsaBI)/ 1 hr.; 65°C (BstBI)/ 1 hr. --> Success! C2925 cells produce non-methylated plasmids. Proceed w/ cloning hygro (PCR) into these plasmids.


 * }