IGEM:University of Debrecen:Formula and making SOB

 Scientific background

Super Optimal Broth   (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli. It was developed by Douglas Hanahan in 1983 and is an adjusted version of the commonly used lysogeny broth. Growth of E. coli in SOB or SOC medium results in higher transformation efficiencies of plasmids.

Super Optimal broth with Catabolite repression </b> <span style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;"> <span style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;">(SOC) is SOB with added glucose.</o:p>

<p class="MsoNormal"> </o:p>

<p class="MsoNormal"> Materials</o:p>

<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto; line-height:normal"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;; mso-bidi-font-family:Calibri;color:black">To make 1L SOB media: </b><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:Calibri; color:black"></o:p>

<li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:    auto;line-height:normal;mso-list:l1 level1 lfo1;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">20 g Bacto-tryptone</o:p> </li> <li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:    auto;line-height:normal;mso-list:l1 level1 lfo1;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">0.5 g NaCl</o:p> </li> <li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:    auto;line-height:normal;mso-list:l1 level1 lfo1;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">5 g Yeast extract</o:p> </li> <li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:    auto;line-height:normal;mso-list:l1 level1 lfo1;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">900 ml distilled H2O</o:p> </li> </ul>

<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto; line-height:normal"><span style="font-size:12.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;; mso-fareast-font-family:&quot;Times New Roman&quot;;color:black"> </o:p>

<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto; line-height:normal"><span style="font-size:12.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;; mso-fareast-font-family:&quot;Times New Roman&quot;;color:black"> </o:p>

<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto; line-height:normal"> <span style="font-size:14.0pt;mso-fareast-font-family: &quot;Times New Roman&quot;;mso-bidi-font-family:Calibri;color:black">Procedure</o:p>

<li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;margin-bottom:    12.0pt;line-height:normal;mso-list:l0 level1 lfo2;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">Shake until the solution dissolves.</o:p> </li> <li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;margin-bottom:    12.0pt;line-height:normal;mso-list:l0 level1 lfo2;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">Add 10 ml 250mM KCl.</o:p> </li> <li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;margin-bottom:    12.0pt;line-height:normal;mso-list:l0 level1 lfo2;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">Adjust pH to 7.0 with 10N NaOH.</o:p> </li> <li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;margin-bottom:    12.0pt;line-height:normal;mso-list:l0 level1 lfo2;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">Bring to a final volume of 1L with H2O. Pour into 100 ml bottles and autoclave.</o:p> </li> <li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:    auto;line-height:normal;mso-list:l0 level1 lfo2;tab-stops:list .5in"><span style="mso-fareast-font-family:&quot;Times New Roman&quot;;mso-bidi-font-family:     Calibri">To each bottle, add 500 ul 2M MgCl2.<o:p></o:p> </li> </ol>