Citizen Science/Open Fluorometer Project/Resources/Novak 2007 Fluorometer

Bill of materials

 * Optics:
 * Blue LED (ETG-5CE490-15)
 * Lenses (collimation + focus) from Geltech molded glass aspheric lenses (Thorlabs Inc) (6.5mm diameter / focal length 3.1mm / numerical aperture 0.68)
 * Excitation filter ET470/40x from Chroma Inc
 * Dichroic mirror T495LP from Chroma Inc
 * Conventional mirror
 * Emittion filter ET525/50m frmo Chroma Inc
 * Silicon photodiode BPW21 from Siemens Inc (sensitive area 7.34mm2 / quantum yield 0.8 / optical sensitivity 10 nN lx-1)
 * Electronics:
 * Lock-in system based on NE555 (ST microelectronics)
 * Operationa amplifier OPA129 (Burr Brown Inc)
 * Demodulator AD630 (analog Devices Inc)

Published performances

 * LOD for fluorescein 2nM
 * Probed volume < 0.5 uL