CRI DNA sequencing and troubleshooting guide

Purification before sequencing

 * purify plasmids with QIAprep Spin Miniprep Kit
 * purify PACs with QIAGEN Plasmid Kit
 * purify PCR with products Shrimp Alkaline Phoshatase and Exonuclease I
 * xoSAP-IT NEW 	1000 reactions 	US78200
 * Exonuclease I 	2500 reactions 	E70073Z
 * Shrimp Alkaline Phosphatase 	500 reactions 	E70092Y
 * purify PCR product with QIAGEN QIAquick PCR Purification Kit

Purification after sequencing
On capillary instruments, clean up products with Edge Biosystems: Edge Gel Filtration Cartidge cat# 42453
 * 1) centrifuge the Edge plates 750 G for 2 min
 * 2) transfer the cartidge to a clean microcentrifuge tube
 * 3) add your sample (check fluid runs into the gel)
 * 4) close cap
 * 5) centrifuge at 750 G for 2 min