IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Lock and Key Decoder/2010/08/11

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8/11

 * Pulled pLac promoter (R0010) from the 37°C room. - 11:00 a.m.

Transformation
 * Transformed sub-system 2a: - 3:45 p.m.
 * 50 μL competent cells
 * 4 μL 2a plasmids
 * 10 minutes at 0°C
 * 45 seconds at 42°C
 * 2 minutes at 0°C
 * Added 1000 μL SOC broth
 * 1 hour on shaker in 37°C room
 * plated onto Tetracycline and LB growth media and left in 37°C room overnight

Inoculation
 * inoculated pLac promoter and GFP - 5:00 p.m.
 * left in 37°C room overnight
 * Matthew Entler 17:02, 11 August 2010 (EDT):


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