PEG purification of PCR products

Overview
This is a simple and cost-effective protocol for purifying PCR products from primers and unused dNTPs. Purified products can be used in any downstream application, such as DNA sequencing.

To make PEG

 * 3g PEG 8000
 * 2.19g NaCl
 * Bring up to 15ml with good water.
 * Lasts for at least 30 days at 4C

Protocol

 * Add 1:1 volume of PEG to PCR product, for example into the reaction tube.
 * Vortex briefly.
 * Warm at 37degrees for 15minutes.
 * Microcentrifuge for 15minutes at max rpm.
 * Pipette off supernatant.
 * Wash pellet with exess 150ul 80% cold ethanol.
 * Spin for 10minutes at at max rpm.
 * Pipette off ethanol.
 * [Optional] Wash with 95% ethanol.
 * Dry down.
 * Re-suspend in 10 to 25ul of good water. Generally, I re-suspend 8μL of a PCR product in 12μL water.