User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/23

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03/23/10

 * &#x2713; Minipreps: KAH142/V0201 (4)
 * &#x2713; Cultures: KAH143 (4)
 * &#x2713; Sequencing: KAH86, 96, 142/V0201

Minipreps > Check with XbaI digest

Sequencing (Genewiz, same day)
 * 1) KAH86 P0001: mistake, reverse primer (re-do)
 * 2) KAH96 P0001: mistake, reverse primer (re-do)
 * 3) KAH142/V0201 3T3: good, but did not read into promoter
 * 4) KAH142/V0201 BGHR: good

--> Re-submit KAH96 and 86 for sequencing (both directions, same day on Thurs.). If KAH96 is missing the promoter, need to re-build KAH142.


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