Talk:Knight:Purification of His-tagged proteins/Denaturing

Lane order:

 * 1) SeeBlue Plus2 Pre-Stained Standard
 * 2) Cleared lysate
 * 3) Flow through (after column binding)
 * 4) Flow through (after column binding, second time)
 * 5) Wash 1
 * 6) Wash 2
 * 7) Eluted protein
 * 8) Cleared lysate from cells not expressing a His-tagged protein
 * 9) Flow through (after column binding, second time) from cells not expressing a His-tagged protein
 * 10) Wash 2 from cells not expressing a His-tagged protein