User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/16

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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12/16/09

 * &#x2713; Digest: confirm KAH105-108 (& /MV2) via E/P digests
 * &#x2713; Minipreps: KAH126-133/MV2 (2 ea); KAH123, 125 (6 ea)
 * &#x2713; Assemblies: KAH117-119; KAH135, 136

Digest check > May have mixed up and mislabeled KAH105-108 (& /MV2). Sequencing results are out of order (see | 12/09/09). Could be cause of last failed transfection experiment.

Results are consistent with the sequencing data: 1 = KAH108, 3 = KAH105, 5 = KAH106, 7 = KAH107 --> Correct labeling on the tubes

Minipreps > Check with E/P digests

--> 37°C/ ~15 min.

Assemblies
 * 1) KAH117: KAH99/(E/S)/2913 &#x2713; + (1) KAH93/(E/X)/8039
 * 2) KAH118: KAH100/(E/S)/3085 &#x2713; + "
 * 3) KAH119: KAH101/(E/S)/3157 &#x2713; + "
 * 4) KAH135: KAH78/(E/S)/3652 &#x2713; + (2) KAH134/(E/X)/4600
 * 5) KAH136: KAH78/(E/S)/3652 &#x2713; + "

> Digests (Fermentas FD)

> Measure conc.'s

> Dephosphorylation (Roche)

> Ligations


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