IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/07/19

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July 19th, 2009
- Entered lab at approximately 1 p.m to check the replates (Kan-GFP-paraoxon biosensor). Looks good, lots of big colonies

- Took 1 colony from each section, replated as before, in cyclic arrangement of 6 triangular units, as diagrammed in notebook (see Nora's book)

- made 3 ml starter culures of 5 colonies from GFP plate (didn't have enough YPD to do all 6) - start @ 2 p.m

- will pick up Monday, make dilutions for GFP test.

July 19, 2009 P2
Checked plates and it seems there are very few cells growing. Pipette more gently, give more time for recovery, and use a different spreading technique.

Transformation of Longtine PNP PCR Products
I performed a transformation yesterday, 720, to see if I could get any good growth with these PCR products


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