IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/02/19

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Constructing the systems and put them together.


-What are the sites for restriction enzymes in the operon?

-Can we change the color expression?
 * OmpR

This part is complemented whit information exposed in operón Lux. We are trying to together these systems.

-What else is regulated by Ompr?

-Search for more information of Ompr.

-What are the phenotypes of Envz?


 * LovTAP

There is a trouble trying to activate the LovTAP operon. We can resolve them trying with Tryptophan auxotroph or a Tryptophan repressor knockout.

-Know about mutants phentoypes.


 * Apoquerin

There is an advantage whit this bioluminescence system because LW of light can active one of the purposed phytochrome.

-Can it be applied in E. Coli?

-What is the best option for Ca +2 influx?

-How can be regulated Ompr?

-Search in literature for mutants phenotypes.


 * Phytochromes.

-Search for their paths and viability.


 * General things.

-Search in literature how much light can be emitted by bioluminescent molecules.

Modeling
-Know all the parameters.