IGEM:IMPERIAL/2007/Notebook/2007-8-1

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/15 view=threemonths format=%name/%year-%month-%day weekstart=7

Preparation of Stock Solutions

 * 1x 1 litre of 10x TBE
 * 20x 100 ml of LB media in conical flasks

Preparation of Competent Cells

 * 1) Poured LB agar plates
 * 2) *Nalixidic acid (15 μg/ml)
 * 3) *Tetracyclin (20 μg/ml)
 * 4) Streaked E.coli cells on agar plates
 * 5) *DH5Alpha
 * 6) *XL1Blue
 * 7) Incubated plates at 37°C overnight

Preparation of Equipment

 * 1x 37°C shaking incubator
 * 1x 4°C fridge
 * 1x -20°C freezer
 * 2x Weighing scales
 * 3x Magnetic stirrer
 * 3x Microcentrifuge
 * 3x Vortex machines
 * 2x UV Spectrometer
 * 5x Gilson pipettes (P1000, P200, P20, P10, P2)
 * 20 boxes x Tips (yellow and blue)
 * 5 boxes x Small tips
 * Eppendorf tubes
 * Centrifuge tubes
 * 50x 5 ml pipettes
 * 50x 10 ml pipettes
 * 50x 25 ml pipettes
 * 5x Pipette fillers
 * 37°C water bath
 * 15°C water bath
 * Driblock
 * 10 packets x Innoculation loops
 * 3x Bunsen burners
 * 3 boxes Matches
 * 3x Agarose gel equipments
 * Microwave oven
 * Transilluminator
 * Camera
 * 3x Scapel (for cutting agarose gel)
 * 1 litre bottles
 * Beakers
 * Conical flasks
 * Volumetric flasks
 * Measuring cylinders
 * Gloves
 * Goggles
 * Markers
 * Sticker labels
 * Paper towels