M9 medium/supplemented

M9 medium also exists in various flavors:

M9-glu contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the basic M9 medium recipe.]

M9-CA contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts.

M9-CAglu contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts.

Knight & Endy Lab

 * M9 minimal medium supplemented with 0.2% Casamino acids and 1mM Thiamine Hydrochloride.
 * Knight lab protocol
 * Endy lab protocol
 * Lucks modified Endy recipe
 * Monie modified Endy recipe