CAPS

Purpose
CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.

Recipe
1X CAPS is 10mM, pH 11

To make 5L of 1X CAPS,
 * 1) Combine the following:
 * 2) * 11.066g CAPS
 * 3) * 4.5L 10% Methanol
 * 4) Adjust pH to 11 with around 5mL 10N NaOH
 * 5) Add 10% Methanol to 5L