User:Wilfred J. Poppinga/Notebook/Wilfreds Project/2009/08/05

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Pure colony

 * Nothing usefull, only a smear where large amounts of cells were applied, no single colonies.

Promotor colonies

 * A selfcloser (pSB3K3)
 * Zn-RBS in pSB3K3
 * Cu-RBS in pSB3K3

ON cultures were purified using Nucleospin® Plasmid (Machery Nagel) and a restriction analysis performed using HindIII and XbaI 2 μL Fast digest buffer (Fermentas) 1 μL HindIII (Fast digest, Fermentas) 1 μL XbaI (Fast digest, Fermentas) 16 μL plasmid DNA Restriction samples were put to an 1% (1xTBE) agarose gel after ~30 min.


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