IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/10/01

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Entry title

 * Competent cells could not be prepared cause OD of cells shot up.
 * inoculated J23119 + E0420 and J23119 + I13507.
 * Inoculated DH5alpha for competent cell prparation.
 * Transformation of:
 * J23119 (amp and Chl)
 * The controls C1 to C8 which did not work the other day due to contamination.
 * J24674 from kit (plate 2; 9D);
 * J24676 from kit (plate2; 5O)
 * J23119 S/P digested and alkaline phosphatased.


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