Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/12

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12 May 2009 Lab Meeting
† Announcements † Flu R01 † Coulter Flu Fraunhofer Project
 * SIGN UP FOR NEW LAB DUTIES WITH SONALI.
 * New lab meeting time T 10a-12pm room 705 starting 5/12/2009
 * PDMS Fixture holder - make more at machine shop. Follow proper cleaning procedures with PU molds.
 * RNA extraction troubleshooting.
 * Mass Spec of elution? Martin Steffen?
 * Bioanalyzer not working.. why? LOD - Serially dilute our samples and look to see if we can see it when we dilute the contaminants and the DNA/RNA
 * Absolute Quantification assays
 * FTIR-ATR look at the eluant?
 * Fraun folks coming to get trained next week. RNA isolation.
 * IBC for Fraun flu. in process.

† SEPSIS Project †RNA project † COBRA
 * Revision in process due 5/20.
 * MM to CMK at the end of this week.
 * Jeff visiting week of June 1st
 * Part last week was leaking b/c of flatness issues. No SERS test.
 * Mask our for SU-8 mold. (PDMS)
 * Test again before next Wed.

paper 1: Evap with Sol Gel substrate. paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?
 * Evap system (JD and JZ)
 * Let's get this to a point where we can publish and move on

† Virus concentration † Valve Array/Valve building - done. † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR
 * ATCC flu virus grew and harvested
 * Plaque assay done.
 * SP students are gone? Alex. JD.
 * Paper in revision Yeah!
 * Bonding meeting with MIT folks - Alex left some samples with them. Updates?
 * Final draft with collaborators.
 * designing herringbone groove for better trapping (Mask writer down until next Tuesday)
 * investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
 * IRB still in revision.
 * repeated test of the PCR efficiency for different designs of channel.
 * test PCR efficiency using smaller DNA size (63bp product).
 * analyze PCR product for different temperature using bioanalyzer.
 * getting statistical data for the activation energy from 50 particle simulation (Fig. 5-7)
 * Mincheol has written the introduction, results and discussion, and conclusion.
 * will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)
 * Mincheol has written the introduction, results and discussion, and conclusion.
 * will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)

† RCA/HDA
 * Giving up on white paper. Think about other grant outlets.
 * Preliminary no-valve design for 25 microliters.
 * 20 more test chips to Sonali (AACC poster).
 * Sonali try to do 10 microliters in the tube * Updates?
 * Jane is working on passive valve characterization: threshold pressure not related to cantilever length or bonding time, try hole size next

† Silica Optimization (Lambda):


 * Run single PCR product from QQ or Sonali the test with the bioanalyzer


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