Fong:LB

Protocol for the preparation of lysogeny broth

Materials

 * Pure water
 * LB powder
 * Agar (if making dishes)
 * Antibiotic stock (if appropriate)

Procedure

 * 1) Weigh out 20g of LB powder and place in a clean 1L bottle
 * 2) Add 18g of agar (only if making semi-solid media in dishes)
 * 3) Fill to 1L with pure water
 * 4) Autoclave solution for 15 minutes on "liquids cycle"

Pouring dishes

 * 1) Add appropriate antibiotic to LB once it has cooled
 * 2) Pour liquid media before it starts to set - cover bottom of dish & just a bit more
 * 3) Label each dish with LB, antibiotic (include concentration if different from standard), date and initials
 * 4) Store in refrigerator within plastic sleeve