IGEM:UNAM/2009/Notebook/IGEM project/2011/06/01

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Grow Escherichia Coli DH5-α
Abstract


 * Today I did three petri dishes with the bacteria Escherichia Coli DH5-α, which has the PBBMRCS-5 plasmid.
 * Besides I did a agarose gel of the PBBMRCS-5 plasmid.

Grow E. coli DH5-α


 * 1. Prepare LB medium.
 * 2. Put 100 μl of gentamicin.

We do three tubes:
 * 1. Control tube -> LB solid medium + gentamicin.
 * 2. Bacteria tube -> LB solid medium + gentamicin + Escherichia coli DH5-α.
 * 3. Bacteria tube -> LB solid medium + gentamicin + Escherichia coli DH5-α.

Note: The PBBRMCS-5 plasmid give to the bacteria resistance to gentamicin.

Gel of PBBMRCS-5 plasmid


 * Line 1st -> 5 μl of Green ladder.
 * Line 2nd -> 2.5 μl of plasmid + 2.5 μl of dye. (1)
 * Line 3rd -> 2.5 μl of plasmid + 2.5 μl of dye. (2)
 * Line 4th -> 5 μl of Green ladder.

Note: Previously the plasmid PBBMRCS-4 was digested with XBA I and Hind II.


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