User:Tara K. Luckau/Notebook/Team ConGen/2011/06/13

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 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
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 * style="background-color: #9DB68C" align="center"|  |Main project page


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Gradient Summary

 * favorable conditions (temperature and buffer) is indicated by green highlighting, and corresponds to the green happy faces on each gel
 * right-most column indicates number of primer pairs that work well under that condition
 * [[Image:20110613 GradientSummary.jpg]]
 * will next try to multiplex 7 primer pairs using Buffer E at 64°C
 * Scun2
 * Scun3
 * Scun9
 * Scun10
 * Scun11
 * Scun15
 * Scun22

Multiplex 1

 * The first attempt at multiplexing!

PCR

 * reaction conditions are based on the gradient summary (above) - Buffer E, 64°C
 * run uniplexes at this reaction condition to check against the gradient PCR result, 4 replicates
 * run multiplex at this reaction condition, 8 replicates
 * hopefully, we'll get clear bands in both uniplexes and multiplex!


 * [[Image:20110613 PCR.jpg|800 px]]

Gel

 * Expected banding (based on gradient PCRs)
 * [[Image:20110613 MP1GelExpectation.jpg|300 px]]


 * [[Image:20110613 Gel.jpg|900 px]]
 * Conclusion: missing the Scun22 band, but the other 6 look like they might be there
 * ready to order labelled primers, and start multiplexing and fragging!


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