User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/07/04

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The Daily Grind

 * Gonna design/order primers today for DGGE - guess I should talk to Bea first.


 * Thinking of using 799f/1492r for 16S (nested from another gene product with primers from here), degenereate primers for nifH (PolFI/AQER-GC30 as found in this publication), and degenerate primers DegACC5′/DegACC3′ for ACC Deaminase based on this publication.
 * PolFI = TGC GAI CCS AAI GCI GAC TC
 * AQER-GC30 = CGC CCG CCG CGC CCC GCG CCC GGC CCG CCC GAC GAT GTA GAT YTC CTG
 * DegACC5′ = GGB GGV AAY AAR MYV MGS AAG CTY GA
 * DegACC3′ = TTD CCH KYR TAN ACB GGR TC


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