Template:SBB-Protocols Enz2

EcoRI/BamHI Digest of PCR Products
For PCR products, you will only digest a portion of your purified PCR product. 8uL of eluted PCR product 1uL of NEB Buffer 2 0.5uL EcoRI 0.5uL BamHI
 * Set up the following reaction:
 * Incubate at 37 degrees on the thermocycler for 1hr
 * Run an agarose gel, and melt with 600uL ADB buffer at 55 degrees. ****NOTE:  If you are running short of time, this is an acceptable stopping point
 * If the DNA is shorter than 300bp, add 250uL of isopropanol and mix prior to loading it on the column