User:Fermenter User/Notebook/BROM C9

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

THIS IS BROM C10 (F4)

Day -1 (Dec 20 Mon):
             12/20/2010 (15:00) Bring concentrated 4-L media to fermenter room (first time prepared, diluted too much. second time prepared again)           12/20/2010 (14:30) Start Fermentation Software for Fermenter #4 12/20/2010 (15:00) Dilute with water to 40-L final 12/20/2010 (15:00) Add 40-ml of Antifoam (10-ml Antifoam/10-L) 12/20/2010 (15:30) Start Sterilization 12/20/2010 (16:00) Complete Sterilization 12/20/2010 (16:10) Cooling of bioreactor (Temp setup) 10 L/min air, 50 rpm

Day 0 (Dec 21 Tue):
12/21/2010 (11:20) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 12/21/2010 (11:20) Set dO2 12/21/2010 (11:20) No Acid and Base (1000 ml) 12/21/2010 (11:20) Start pH control     12/21/2010 (13:00) Inoculation: inoculation volume (1800 ml) Fermentation 0:00              12/21/2010 (13:00) Sampling  Fermentation 0:00 Cell weight (??? mg), Activity ( 95.2%, x10), Activity ( 67.3%, x100)

Day 1 (Dec 22 Wed):
           12/22/2010 (10:30) Fermentation 21:30 Refill Base (+1000 ml)              12/21/2010 (10:30) Sampling  Fermentation 21:30 Cell weight (??? mg), Activity ( 86.7%, x100), Activity ( 49.4%, x1000)              12/21/2010 (11:30) Sampling  Fermentation 22:30 Cell weight (??? mg), Activity ( 86.2%, x100), Activity ( 52.0%, x1000)              12/21/2010 (13:30) Sampling  Fermentation 24:30 Cell weight (??? mg), Activity ( 83.3%, x100), Activity ( 39.6%, x1000)              12/21/2010 (15:00) Sampling  Fermentation 26:00 Cell weight (??? mg), Activity ( 84.6% x100), Activity ( 40.6%, x1000)     12/21/2010 (15:40) Harvest


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