User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/17

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Alien genes
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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PEG precipitation

 * recipe:
 * 3g PEG 8000
 * 2.19g NaCl
 * Bring up to 15ml with good water.
 * added 8ul PEG to PCR
 * spun at max speed for 15 min
 * removed PEG, added 150ul cold 80% ethanol
 * spin 7 min
 * remove ethanol, dry
 * re-suspend in 10 ul water
 * nanodrop