IGEM:metu/2009/Notebook/wound dressing/2009/08/29

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29.08.2009
1. Isolations of 1,2,3,4,5,6,7,8,9,10,11,BB were made.

2. Digestions of 1 and 2 were made control 1,           Buffer                  5 µl                                2  µl Spe1                   5 µl                                0 DNA                   40 µl                               10 µl water                  0        =50 µl                     8 µl       =20 µl control 2,           Buffer                  5 µl                                2 µl Pst1                   5 µl                                0 DNA                   40 µl                               10 µl water                  0        =50 µl                     8 µl       =20 µl

3. Electrophoresis order; 1)leader / c1/ 1 / 11 / s1/c2/ 2 / 22/ s2 / leader  2)leader / c1/  1 / i1 / p1/c2/ 2 / i2/ p2 / leader

4. We couldn't digest.