IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/12

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Entry title

 * Miniprep done for the 17 parts.
 * Ultra competent cells of DH5a and DB3.1 prepared and stored at -80 degrees Celsius.
 * OD for Dh5a was 0.4 and 0.9 for DB3.1 (protocol states that it should be about 0.3 to 0.5); LB was used as blank whereas media used for inoculation of cells was SOB.
 * Transformation of DB3.1 with the following 5 Plasmid backbones :
 * pSB1A3
 * pSB1C3
 * pSB3C5
 * pSB4A5
 * pSB4C5
 * Also transformed the following parts
 * K117005
 * B0034


 * Poured Agar Plates (Amp and Chl)


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