User:Tk/Notebook/MF/2008/09/12

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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Clontech In-Fusion protein identification

 * Recycled In-Fusion 2.0 CF dry down kit from Drew's lab
 * Dissolve tube material in 50 ul water
 * Run 20 ul + 5 ul LDS loading buffer, heated to 70C
 * Run also 5x dilutions
 * No DTT or other reducing agent.
 * Bands at 120 and 63 kD
 * Also a very bright band at the well edge, probably unreduced protein
 * Run was with MES buffer, retry with MOPS buffer to give better separation.
 * Pre-run 30 min, run 1:30 at 150 V, about 13 watts


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