Paulsson:Plasmids

=Plasmids=
 * SeqBuilder files for many plasmids can be found in the PAULSSON LAB \ DNA sequences shared folder
 * Key to drug resistance markers: Ap (ampicillin, BLA gene), Cm (chloramphenicol, CAT gene), Kn (kanamycin, APH gene(?))
 * "Ptet" and "Plac" are from Lutz and Bujard ('97) and are actually modified Plambda promoters that have tet and lac operators, and are constitutive in the absence of TetR and LacI. Names should be changed to be more acurate, as in Lutz and Bujard: PLtetO-1 and PLlacO-1

T-series plasmids
Generated by digesting backbone (# before T) with ClaI and non-directional insertion of ClaI digested TetR PCR product. Selected non-fluorescent transformants. Apparently ClaI had STAR activity and religation yielded many plasmids that were non-fluorescent because they had lost the YFP sequence, not because they had TetR. TetR PCR product has not been sequenced.