User:Karmella Haynes/Notebook/miR Trigger/2010/05/03

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05/03/10

 * CFP Reporter + JDS33-47 lines: puro selection plates

CFP Reporter lines > Set up puro selection plates (25 mL) for two lines (FC# and FC#) > ~100,000 cells per plate > 6-well back-up non-clonal plate (1.0 μg/mL puro)
 * 1) 1.0 μg/mL puro
 * 2) 1.5 μg/mL puro (JDS33-47 parent line is puro resistant, try increased puro)


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