Quint Lab:yeast mating in 24-well plates

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yeast mating in 24-well plates
modified Clontech protocol #PT3024-1 IX/C for microtiter plates

or or
 * 1) add 500 µl 1x YPDA (2x YPDA also works) to each well
 * 2) add 20 µl prey overnight culture
 * 3) add 20 µl bait overnight culture
 * 1) pick one colony of the bait and one colony of the prey and stir them into the YPDA
 * 2) place plate on a rotating platform shaker
 * 3) incubate @ 30 °C @ 120 rpm for 6 to 20 hrs
 * 4) plate 100 to 200 µl of the mating culture on a 8 cm plasmid selective plate
 * 1) if you know the mating works, plate 20 to 50 µl on ¼ of an 8 cm plasmid selective plate
 * 2) incubate for 2 to 5 days @ 30 °C to allow diploid cells to form colonies
 * 3) streak over and score on selective media