20.109:Module 1:RefactorM13

Discoverers

 * Rebecca Adams (BsmBI site, rbs-g10)
 * Andrew Ji
 * Bryan Hernandez
 * Amirah Khan
 * Stephanie Nix
 * Mathangi Radha

Designers

 * Kevin Vogelsang
 * Bryan Hernandez
 * Kate Broadbent
 * Aditya Kohli
 * Matt Gethers
 * Jerzy Szablowski
 * Neil Zimmerman
 * Jen Chao
 * Tiffany Guo
 * Sophie Wong
 * Mary Hatch
 * Han Zhu
 * Jen Logan
 * Emzo de los Santos
 * David Ying
 * Mike Lee
 * Mike Oh
 * Dawn Spelke

Annotation
We'll need to annotate the M13K07 DNA sequence that's online here.


 * Kevin T.L. Vogelsang: (gene VI, HpaI restriction site)
 * Bryan Hernandez: BBa_m1307, gene VIII, and AhdI site
 * Jen Logan: (promoter g5, PvuI site)
 * Aditya Kohli: BBa_M13508 (rbs-g8), AccI site
 * Kate Broadbent: BBa_M13507 (rbs-g7), BamHI site
 * Matt Gethers: BBa_13102 (Gene 2 Promoter) and BssSI Site
 * Jerzy Szablowski: MscI site [5080-5085], BBa_M13505 (rbs-g5) [827-842]
 * Neil Zimmerman: HindIII site (7402-7407), BBa_M13503 (g3 RBS: 1563-1578)
 * Amirah Khan: BBa_M13103 (Gene 3 Promoter: 1500-1547) and BsrGI site (1021-1025)
 * Sophie Wong: BBa_M13108 (Gene 8 Promoter: 1155-1201), Pst1 site (8079/8083)
 * Han Zhu: BBa_M13502 (Gene 2 RBS: 8252-8267), TliI site (7922/7926)
 * Andrew Ji: BBa_M13504 (Gene 4 RBS: 4204-4219), BstBI site (6518/6520)
 * Iny Jhun: BBa_M13001 (Geme 1: 3196-4242, PacI (4131-4138)
 * Tiffany Guo: BBa_M13501 (Gene 1 RBS: 8252-8267), SgrAI (5867-5871)
 * Mathangi Radha: BBa_M13106 (Gene 6 Promoter: 2716-2764), NgoMIV (5613/5617)
 * Mike Lee: BBa_M13003 (Gene 3 ORF: 1579-2853); ApaLI site (4743-4748)
 * Emzo de los Santos: BBa_M13005 (Gene 5 ORF: 843-1106), SacI site (6951/6956)
 * David Ying: BBa_M13101 (Gene 1 Promoter: 3086-3132), SacII site (6030-6035)
 * Dawn Spelke: BBa_M13104 (Gene 4 Promoter: 4055-4103), NruI site (7865/7870)
 * Stephanie Nix: BBa_M13110 (g10 promoter: 381-428), XhoI site (7922/7926)
 * Michael Oh: Bba_M13506 (rbs-g6: 2840-2855), SwaI site (8202-8209)
 * Jen Chao: BBa_M13509 (gene 9 RBS) ,EcoNI site (7611-7612)
 * Mary Hatch: BBa_M13504 (gene 4 RBS), BBa_M13511 (gene 11 RBS), DrdI site (5763/5765)

Suggested redesign ideas from "for next time" assignment M1D2
moved to discussion page for M1D3

Clean up work on wild-type genes
Note: modified parts would need to be codon varied to remove direct repeats (for cases in which internal parts are unstuffed).

Please add your thoughts about this M13.1 sketch and the class redesign ideas. ''' All ideas are welcome. ''' Preceed your comments with 4"~"s to allow for attribution. Nkuldell 11:37, 23 February 2007 (EST) I don't think we want to make M13 an integrating phage based on work of [Derbise, et al]