User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/04/09

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Continue collecting virgins for stable control line.
 * Continued dissections
 * Wash 3 x 15 minutes in 1x PBST
 * Incubate with 1:500 goat-anti rabbit 488 secondary antibody in 2% NGS
 * 2hr at RT
 * Wash 3 x 15 minutes in 1x PBST
 * Leave in 2% NGS at 4C over weekend.


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