IGEM:IMPERIAL/Protocols/2006/western blot


 * Grow overnight in a 5 mil culture
 * Make a fresh day culture 400ul of overnight culture + 4.6 ml of fresh medium (LB)
 * Grow fresh culture for 1.5hrs
 * Remove 1ml This will be your controll
 * Add IPTG to the remaining 4ml Final conc should be 0.5-1 milimolar So add 4ul of 1 molar IPTG
 * Grow for 2-3 hrs
 * Centrafuge down
 * Go to Keran's Lab (on the same floor as kirstin)
 * Add Lysis Buffers
 * Run on gel

You should get this

Source http://www.cdc.gov/ncidod/eid/vol7no1/hussainG1.htm