User:Fermenter User/Notebook/HANC A4 F4

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HAND A4 (F4)

Day -7 (Nov 5 Wed)
           11/5/2008 12:00  Start Steam Generator. 30 psi maintained.

Day -6 (Nov 6 Thu)
           11/6/2008 12:13  Calibrate pH probe            11/6/2008 12:30  Add Media ( (NH4)2SO4 46.25g, K2HPO4 347g, KH2PO4 149.5g) pH adjust to 7.0 Refill water to 50-L 11/6/2008 12:45 Steam Generator Malfunction!! Plant Ops called. Fermentation Ceased until Steam Generator is Fixed!!

Day -5 (Nov 7 Fri)
          11/7/2008 (14:00)  Peter Vickers came by and Opened main valve of steam generator which was closed.            11/7/2008 (14:08)  Sterilization Started Successfully! 11/7/2008 (15:08) Sterilization completed and Vessel is cooled down to 20C 11/7/2008 (15:08) Purge vessel with 50L/min Air

Day 0 (Nov 12 Wed)
           11/12/2008  (11:00)  Set temperature of vessel to set point (37C) 11/12/2008 (12:20)  Add other media components: 50 mM MgSO4 500 ml                                                  2M Arginine 500 ml                                                   10 mM FeSO4 50 ml                                                   No Antifoam added 11/12/2008 (12:30)  Calibrate dO2 probe             11/12/2008  (14:00)  Bring cells to fermenter room            11/12/2008  (14:00)  Inoculate cells into fermenter Fermentation time 0:00 Increase rpm to 300, Decrease Air flow to 10L/min 11/12/2008 (14:30)  Add 10 ml of Antifoam

Day 1 (Nov 13 Thu)
           11/12/2008  (12:00)  Harvest 70-L fermenter using CEPA Z-41 continuous centrifuge             11/12/2008  (13:00)  Come to pick up cell pellet


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