User:Morgan L. Paull/Notebook/Genetic Fence/2011/01/19

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Checking Genetic Fence Plasmids

 * Running digests to confirm the status of biobrick plasmids from igem team fence over the summer and into the fall. If the proper-sized cutout appears, I will keep the plasmid checked and discard the others so as to consolidate the materials in the fence boxes down to one or two boxes.
 * digested each plasmid with EcoR1 and Pst1 to confirm the presence of the widest parts of the biobrick sites as well.
 * used between 1 and 3μL of plasmid, depending on concentration, in an attempt to stay at reasonably concentrations of DNA.

First (top) Row

Second Row


 * each successive image follows the gel running an additional 15 minutes after the initial 15 minute run
 * Summary: at least one copy of each part is good except for Act2LacO (which has the same old problem with the weird two bands) and LacIN-Nost-Act2LacO, which probably is a result of the fact that the LacO from which it was cloned has that weird band (and did at the time of cloning as well)

To Do

 * do a series of diagnostic digests on Act2LacO with different enzymes and also without any restriction enzymes
 * based on the results of that, prepare to re-ligate LacIN-Nost-Act2LacO (which I need as a whole part to do gibson on the whole fence)