IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-22

double digesting test of E0040->pSB3k3

 * digesting (GFP+pSB3K3) and E0040 with EcoR1/PstI.
 * Digestion system contains :
 * 1) (GFP+pSB3K3): 2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 5uL plasmid, 13uL ddH2O.
 * 2) E0040:2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 4uL plasmid, 13uL ddH2O.
 * 37℃ culutre for 2 hours.

electrophorsis result

 * from left to right:
 * 1) e0040->pSB3k3-1 @ EcoRI/Pst1
 * 2) e0040->pSB3k3-2 @ EcoRI/Pst1
 * 3) control:e0040 @ EcoRI/Pst1
 * 4) I7100 @ EcoRI/Pst1
 * 5) e0040->pSB3k3-1 plasmid
 * 6) e0040->pSB3k3-2 plasmid
 * 7) Marker: DL2000 plus




 * I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.