Wittrup: Plasmid rescue from yeast

Use zymoprep I kit or zymoprep II kit  from ZymoResearch.

Zymoprep I: Modifications to manufacturer's protocol -- Resuspend pellet in 17 uL TE and use 1-2 uL for XLI Blue transformation.

Zymoprep II: Modifications to manufacturer's protocol -- Spin down supernatant in fresh tubes a second time before applying to column. -- Elute column in 20 uL TE, and then clean this product on a Qiagen column.