Registry/Measurement kit/Notebook/2007-6-20

To Do

 * 1) Add new devices into the Registry
 * 2) Check re-plating of devices A and C (done. no growth.)
 * 3) Create/order primers for device B
 * 4) Mini-prep and sequence device B culture (colony 8) and Jason's three cultures (done)

Mini-Prep

 * Spun at 5k for 4.5 min before prepping
 * note: accidentally added 250 of P1 to each tube instead of each culture, but reconsolidated into four miniprep columns. We'll see if this affects the results drastically.
 * Eluted with 30µL of EB.
 * For future reference: when sequencing, should elute with aqueous NaOH solution of pH 8.5
 * Will probably need to re-do this...

"Device B" Primers

 * Fwd
 * CTTAGTAG + CAATTG + tccctatcagtgatagagattgacatc
 * Rev
 * TCAGCGAT + ATGCAT + TATAAACGCAGAAAGGCCCAC

Tm ~53 deg