Freimoser:Recipes:TransferBuffer

Final working concentration (1x)

 * 3  g  Tris
 * 14.4 g Glycine
 * 800 mL H2O
 * 200 mL Methanol

10x stock solution

 * 30  g  Tris
 * 144 g  Glycine

Fill up to 1000 mL with H2O.

To make the 1x working solution from this stock use:
 * 100 mL 10x stock solution
 * 700 mL H2O
 * 200 mL Methanol