Van Oudenaarden Lab:C17C3.7

Progress:

Probes designed: (03-04-2010 - Christoph Engert)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

--- >C17C3.7 (spliced + UTR - 807) ATGCCAAAAG TTATTCAGTC TTCAATGAGC GATTATCGTT CCGTCCCATA CAATCAAACT CCAAAATCAG CTTCTGAACG

GAAAAGAAGA AATATTACAA ACGAGTTGAT TAACGAATGC AAGACGATTG TTCAAAAATC AGAAGAAGAA CATATATCAC AAGAAGTAGT TCTCTTCCGA ATTGTGAAAC TTGTGACTGG AGTTAATCTG GAATCTAATT TCTCATCGAA TGACCTCTCC GAATCTACAA GACGGAAGTT TGATACTGAA TCCGAAAGAA GAAAAGTGAA GACTGAACGT GAAAAGATTC GAAGAAAGAA ACAAGATGAC TGTTATGCTG AATTGAAATT CTTCATTTTG AACAAACAAA TGGGAAGTTA CGAGCAACGA TTAAAACTTG AACGGATCAC AATTCTAGAA ATCATCATTG ATTACATTAA ACACAATTCC GATCTTCTGT ATCCAGAAAC AATTCCCCAA ATACTTCCTC TTTTAGCTGG AAAATCAACT GCTACTTGTG AGAACAAAGA GAATGAAAAG CCAAAAACAC GAATGGAAGT GAAGGATCTC TTCCCAAGAT TGACGTTTCA AGAAGTTCAA GAATCCCCAA CATCTACTTC TCCATTGCTC ACATTTCCAT GTATTCCAAT GATCCCAACT ACTCAGTTCA ATGTCTTGTC AAACTACAAC ACCGTTCCAT CAATATTTTC TGCTCCCCTC CGATTCATTC TTCCATCACT TCAAATCTTA ACACCAGAAA CTAGTGACGA GGAAGAAAAT GAAGAAACCC TCGATATAAT TAACTAG

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Name of probe set: hlh-25 Probes designed on Wed, 03 Mar 10 22:55:07 -0700 32 probes designed for target of length 807

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

gactgaataacttttggcat, hlh-25_1, 1, 1, 35 ggaacgataatcgctcattg, hlh-25_2, 2, 23, 45 ttggagtttgattgtatggg, hlh-25_3, 3, 45, 40 cttttccgttcagaagctga, hlh-25_4, 4, 67, 45 gcattcgttaatcaactcgt, hlh-25_5, 5, 101, 40 ctgatttttgaacaatcgtc, hlh-25_6, 6, 123, 35 cggaagagaactacttcttg, hlh-25_7, 7, 160, 45 ctccagtcacaagtttcaca, hlh-25_8, 8, 183, 45 gaggtcattcgatgagaaat, hlh-25_9, 9, 218, 40 acttccgtcttgtagattcg, hlh-25_10, 10, 240, 45 cttctttcggattcagtatc, hlh-25_11, 11, 262, 40 ttcacgttcagtcttcactt, hlh-25_12, 12, 284, 40 cttgtttctttcttcgaatc, hlh-25_13, 13, 306, 35 ttcaattcagcataacagtc, hlh-25_14, 14, 328, 35 cttcccatttgtttgttcaa, hlh-25_15, 15, 358, 35 caagttttaatcgttgctcg, hlh-25_16, 16, 381, 40 atttctagaattgtgatccg, hlh-25_17, 17, 403, 35 cagaagatcggaattgtgtt, hlh-25_18, 18, 440, 40 tttggggaattgtttctgga, hlh-25_19, 19, 462, 40 tttccagctaaaagaggaag, hlh-25_20, 20, 484, 40 gttctcacaagtagcagttg, hlh-25_21, 21, 506, 45 ccattcgtgtttttggcttt, hlh-25_22, 22, 537, 40 tcttgggaagagatccttca, hlh-25_23, 23, 560, 45 cttgaacttcttgaaacgtc, hlh-25_24, 24, 582, 40 atggagaagtagatgttggg, hlh-25_25, 25, 606, 45 ggaatacatggaaatgtgag, hlh-25_26, 26, 628, 40 gaactgagtagttgggatca, hlh-25_27, 27, 650, 45 cggtgttgtagtttgacaag, hlh-25_28, 28, 675, 45 ggagcagaaaatattgatgg, hlh-25_29, 29, 697, 40 gtgatggaagaatgaatcgg, hlh-25_30, 30, 720, 45 cgtcactagtttctggtgtt, hlh-25_31, 31, 750, 45 cgagggtttcttcattttct, hlh-25_32, 32, 774, 40 -- Input sequence with probe locations:

atgccaaaagttattcagtcttcaatgagcgattatcgttccgtcccatacaatcaaactccaaaatcag tacggttttcaataagtcag gttactcgctaatagcaagg  gggtatgttagtttgaggtt  agtc Probe # 1, 35% GC    Probe # 2, 45% GC     Probe # 3, 40% GC     Prob

cttctgaacggaaaagaagaaatattacaaacgagttgattaacgaatgcaagacgattgttcaaaaatc gaagacttgccttttc             tgctcaactaattgcttacg  ctgctaacaagtttttag e # 4, 45% GC                Probe # 5, 40% GC     Probe # 6, 35% GC

agaagaagaacatatatcacaagaagtagttctcttccgaattgtgaaacttgtgactggagttaatctg tc                gttcttcatcaagagaaggc   acactttgaacactgacctc Probe # 7, 45% GC     Probe # 8, 45% GC

gaatctaatttctcatcgaatgacctctccgaatctacaagacggaagtttgatactgaatccgaaagaa taaagagtagcttactggag gcttagatgttctgccttca  ctatgacttaggctttctt Probe # 9, 40% GC    Probe # 10, 45% GC    Probe # 11, 40% GC

gaaaagtgaagactgaacgtgaaaagattcgaagaaagaaacaagatgactgttatgctgaattgaaatt c ttcacttctgacttgcactt  ctaagcttctttctttgttc  ctgacaatacgacttaactt Probe # 12, 40% GC   Probe # 13, 35% GC    Probe # 14, 35% GC

cttcattttgaacaaacaaatgggaagttacgagcaacgattaaaacttgaacggatcacaattctagaa aacttgtttgtttacccttc  gctcgttgctaattttgaac  gcctagtgttaagatctt Probe # 15, 35% GC    Probe # 16, 40% GC    Probe # 17, 35% GC

atcatcattgattacattaaacacaattccgatcttctgtatccagaaacaattccccaaatacttcctc ta                ttgtgttaaggctagaagac  aggtctttgttaaggggttt  gaaggag Probe # 18, 40% GC   Probe # 19, 40% GC    Probe #

ttttagctggaaaatcaactgctacttgtgagaacaaagagaatgaaaagccaaaaacacgaatggaagt aaaatcgaccttt gttgacgatgaacactcttg           tttcggtttttgtgcttacc   a 20, 40% GC    Probe # 21, 45% GC             Probe # 22, 40% GC     P

gaaggatctcttcccaagattgacgtttcaagaagttcaagaatccccaacatctacttctccattgctc cttcctagagaagggttct ctgcaaagttcttcaagttc    gggttgtagatgaagaggta  gag robe # 23, 45% GC   Probe # 24, 40% GC      Probe # 25, 45% GC    Pro

acatttccatgtattccaatgatcccaactactcagttcaatgtcttgtcaaactacaacaccgttccat tgtaaaggtacataagg actagggttgatgagtcaag     gaacagtttgatgttgtggc  ggta be # 26, 40% GC   Probe # 27, 45% GC       Probe # 28, 45% GC    Prob

caatattttctgctcccctccgattcattcttccatcacttcaaatcttaacaccagaaactagtgacga gttataaaagacgagg  ggctaagtaagaaggtagtg          ttgtggtctttgatcactgc e # 29, 40% GC    Probe # 30, 45% GC            Probe # 31, 45% GC

ggaagaaaatgaagaaaccctcgatataattaactag tcttttacttctttgggagc Probe # 32, 40% GC