IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/07

{| width="800"
 * style="background-color: #800000"|[[Image:UIUC_ArsR_System.jpg|150px]] UIUC - Arsenic Collection System!
 * style="background-color: #AFEEEE" align="center"|  |Main project page
 * style="background-color: #AFEEEE" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Current Project Summary
NOTE: need to order restriction enzymes: BsaI, but leave BamHI and KpnI until later

7/7/10
PCR Purified pArsR to 50 ng/ul. Only used 15 ul to purify, should have about 10 ul left in tube. Used purification product for digestion.

Digestions

pArsR

pSB1T3 Incubate at 37°C 15 min. Inactivate at 80°C 20 min.

PCR purified pSB1T3: No yield.

PCR for pArsR

95 - 2min (95 - 30sec; 55ish - 30sec; 72 - 1min | 31x) 72 - 5 min 4 - hold

Samples:

Swiped tip over Escherichia coli MG1655. Add 25ul dH2O Incubate at 98C for ~2minutes Yield was ~250ng/ul Gel of PCR
 * Colony Boil:

Run on 1% gel




 * }