Kubke Lab:Research/CND/Records/MH002

=Embryo details=

Species: Gallus gallus domesticus Embryo Name: MH002 Embryo stage: ST28 (confirmed by Fabiana) Staging description: Fixation: PF Cryoprotection: Yes Material label and storage: Stored in a vial containing PBS in 30% Sucrose solution overnight at room temperature ON THE 2/12. On the 3/12 the embryo was stored in the histology lab freezer. The slides are labelled MH002 and stored in a brown folder in Fabiana's lab. - Done

=Experiment details=

Objective: To determine what are the best parameters to cut a stage 28 embryo. Procedure: (Satya had left it on 0 as a "default" knife angle. It was moved just above 0 by you (approximately 0.5). I will check my notes to be sure .)--Malisha Hettiarachchi 16:02, 3 February 2011 (EST)

Comments: Incubated the mould in the cryostat for 30 minutes at a temperature of  -23 C OT/CT before placing  it on the chuck

=Results=
 * H&E stained sections following this procedure.
 * Fabiana's feedback: Large holes in the sections which are not expected. Cannot determine if the issue is with staining or sectioning. ( Corresponding to the following entry) --Malisha Hettiarachchi 19:27, 31 January 2011 (EST).

=Images= =Summary=