IGEM:IMPERIAL/2006/Results/ABS490 Calibration

Aim
The aim of this experiment was to make a calibration curve to be able to convert Abs490, which is measured by the plate reader, into OD600.

Method

 * A dilution series of different OD600s was created using the in-lab spectrophotometer and overnight culture of cells.
 * These dilutions were also assessed via the spectrophotometer to make sure they were near their targetted OD.
 * 3 x 200 of each dilution was pipetted into a 96 well plate and then Abs490 was read by the Victor3 plate-reader
 * The cells and the dilutions were kept on ice to prevent any increase in OD

Results

 * Data File:
 * Abs490 -> OD600 Calibration Data