WangLab:HEPES buffer

HEPES

 * 4-(2-Hydroxyethyl)-1-piperazineethanesuffonic acid


 * C8H18N2O4S


 * Molecular weight = 238.30

HEPES buffer

 * 20mM HEPES buffer, PH=7.3


 * x/(238.30*0.1)*10^3 = 20


 * x = 0.4766g


 * 100ml H2O


 * 1mol/L NaOH


 * x’/(40*0.1) = 1.0


 * x’ = 4g


 * 2g : 50ml H2O, 1g, 25ml H2O