IGEM:Peking/2007/Switch-Notebook/2007-8-21

colony PCR to test lacI-pcc009(old)






no one seems to be right!

re-ligation of pcc009 and lacIq
transformation tonight

re-PCR ss1, rs1, rs2, rd2, rd3, cd1


from left to right is CD1, SS1, RD2. RD3, RD3, RS1, RS2

lacI double digestion
lacI 10uL buffer H 10uL XhoI 2uL SalI 2uL ddH2O 76uL total 100uL

pcc009 digestion
XhoI and SalI



the left one is pcc009-1, 0.25uL enzyme/20uL the right one is pcc009-3, 2uL enzyme/100uL