User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/19

 .todo { color: red } .done { color: green} {| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Summary

 * qPCR hTERT, HEK and Jurkat for AKAP9
 * 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H2O)
 * 14 μL each well + 1 μL cDNA
 * Pierce for HBE cells (see attachment)
 * Blots of 10% gel

Attachment
   [[Media:19102010_Pierce_Lysates_HBE.xls| Pierce resultaten HBE (& Jurkat)]]


 * }