User:Karmella Haynes/Notebook/Polycomb project/2010/06/06

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

06/06/10

 * &#x2713; ChIP: order primers for ChIP-DNA PCR
 * &#x2713; Senescence assay optimization: FTRx cell rotenone treatment
 * &#x2713; Growth assay: 12-well plates for growth assay (from 10cm plate cultures -/+ dox)

ChIP Primers > Genes (see spreadsheet for sequences)
 * 1) p15 INK4b
 * 2) p14 ARF
 * 3) p16 INK4a
 * 4) ATF3
 * 5) CCND2
 * 6) GAPDH

Senescence assay > FTRx cell rotenone treatment > 12-well plate; ~100k cells/ well Note: Each row will be treated w/ different concentrations of C12-FDG > Grow at 37°C/ 3 days > Next step: Wed, 6/09; C12-FDG treatment & microscopy
 * 1-4: 0, 0.1, 0.2, 0.4 μg/mL rotenone
 * 5-8: 0, 0.1, 0.2, 0.4 μg/mL rotenone
 * 9-12: 0, 0.1, 0.2, 0.4 μg/mL rotenone

Growth assay > 12-well plates; ~100k cells/ well Plate 1, 2 Plate 3, 4
 * 1-4: 126-1
 * 5-8: 126-3
 * 9-12: 132-8
 * 13-16: 154-2
 * 17-20: FTRx
 * 1-4: 126-1 +dox
 * 5-8: 126-3 +dox
 * 9-12: 132-8 +dox
 * 13-16: 154-2 +dox
 * 17-20: FTRx +dox

> Refresh dox+ today and every 2 days > Do cell counts tomorrow and every 2 days


 * }