IGEM:PennState/Labbook/NoahJohnson/2007-8-2

August 2, 2007
 * Ordered UV cuvettes
 * Began growing up 6 3ml cultures of crp* in LB
 * Will TELT dna prep them tonight or tomorrow

M9 Minimal Media (250mL each):

For ~250mL of media and a final 0.2% sugar concentration:
 * 200mL sterile water


 * 50mL of 5X M9 salts
 * 12.8g Na2HP04*7H2O
 * 3g KH2PO4
 * 0.5g NaCl
 * 1g NH4Cl
 * Dissolve in 200mL deionized water and autoclave to sterilize
 * 3.75g BactoAgar

Autoclave to sterilize and allow to cool until you can hold it for 2 seconds on the palm of your hand. Then add the following and pour into plates:


 * 25mL sugar solution
 * Add 0.5g glucose/xylose to 25mL sterile water
 * Filter-sterilize before adding
 * Makes a 12.5% sugar solution and a 0.2% overall glucose/xylose media
 * 500 uL 1M MgSO4
 * Dissolve 24.65g MgSO4*7H20 in 100mL H20
 * Autoclave to sterilize
 * 25uL 1M CaCl2
 * Dissolve 14.7 g CaCl2*2H2O in 100mL H2O
 * Autoclave to sterilize

I will make 250mL of each of the following:
 * 0.2% glucose
 * 0.2% glucose + 0.2% xylose
 * 0.2% xylose
 * 0.2* glycerol (- control)

Protocol for plates


 * DNA prep on crp* cultures from this morning using TELT method

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