IGEM:MIT/2006/Notebook/2006-9-1

to do

 * 1) glycerol: all LCs -done
 * 2) start a smell test: add precursor to the LCs of 0-Q-119 and O-Q-199 -done, however, results don't look good
 * 3) am miniprep (12): J45397 (1), J45398 (1), J45399 (3), J45299 (3), J45319 (3), pE3 (1) -done
 * 4) analyze: incoming 20 sample sequencing order -done
 * 5) *throw out bad sequences -done
 * 6) digest: J45299 (3) and J45319 (3) with XP -done
 * 7) *run a gel
 * 8) sequence: (32 wells) -done
 * 9) *J45220-C-mut with ATF1 primers -done
 * 10) *all other minipreps (except pE3) -done
 * 11) *J45398-no mut: 3,4,6,7 -done
 * PCR: pmsCEAB out of pE3(new) and pE3R -done
 * 1) *adjust annealing time, check GC content, ask advisor advice on PCRing large coding regions -done
 * 2) *clean up??
 * 3) *run a gel
 * 4) graph the plate reader data (call stephen)
 * 5) 3-part ligate/transform into A/C backbone:
 * 6) *J45299:XP with R0011:ES
 * 7) *J45319:XP with R0011:ES
 * 8) pellet: yeast cells in big centrifuge, resuspend in milk, try baking
 * 9) *ask samantha for help
 * 10) pm miniprep: iGEM yeast
 * 11) *follow yeast miniprep protocol, vortex cells first, ask samantha for help
 * 12) *we can do XS digest/freeze of yeast miniprep on monday and ligate/transform with ATF1 when samantha is around and when we have tried out the bread machine