Endy:Screening plasmid/Inverter characterization

Already measured with SP1.0 (I13534.pSB1A2)

 * Q04400.007 - mutated Q04400 (tetR QPI), point mutation in RBS. Works well, switches later than Q04400.
 * mnt inverters from enterobacteriophage p22 Knight Stormo
 * Note: The promoter used in these inverters is missing base 13 in its 17-bp operator site Stormo, though I think they meant to include it (they reference the article and mention it in the registry). It's also missing a -35 site.
 * Q04720 - mnt inverter, strong repressor - output not very high, and doesn't appear to be doing much (picture)
 * Q04730 - mnt inverter, weak repressor - output not very high, and doesn't appear to be doing much (picture)

Measured with older versions of screening plasmid (I13537.pSB1A2, I13538.pSB1A2, or I13534.pSB4A3)

 * tetR inverters Elowitz Lutz
 * Q04400 - works reliably (quantitatively), switches at low input.
 * Q03400 - weaker RBS driving tetR. Looks like it starts to switch, but very high GFP gives very high RFP numbers. Unclear what's happening.
 * Q01400 - weakest RBS. Generally looks like it's stuck in the high-output stage. Some evidence suggests it might be switching, however.
 * p22 cII inverters
 * Q01530 - p22 cII inverter. Appears to be stuck in the low-output stage, perhaps due to crosstalk with CW2553/lambda.
 * Q04530 - Same as Q01530, but with a stronger RBS driving the cII. Also stuck in low-output.
 * penI inverters Himeno Himeno_errata from bacillus licheniformis
 * Q02740 - strong RBS, looks like it begins to switch at high input.
 * Q03740 - medium strength RBS, stuck on.
 * Q04740 - strongest RBS, works well, switches at higher input than Q04400. [[Image:PenI_QPI.png]]

To be measured/constructed/etc

 * I14103 (Q04740-Q04400.007) - bistable switch one way
 * I14104 (Q04400.007-Q04740) - bistable switch the other way - having trouble getting it cloned in this direction (tetR-penI) - weird stuff happening with growth rate and trying to prep the final plasmid, though colony PCR looks fine - load issues?
 * Q04510 - lambda cI inverter, ligated into the screening plasmid, but somehow missed being characterized. Seems unlikely to work, but hasn't been tested.
 * Q07400/Q08400/Q09400 - tetR inverters using RBS's from the Voigt lab. Not BB'd, and previous attempts were unsuccessful.