IGEM:Yale/2010/Protocols/ethanol precipitation

Standard DNA Precipitation Protocol

 * 1) Add one-tenth volume of 3 M NaOAc, ph 5.2, to the DNA solution to precipitated.
 * 2) Add 2.5 volumes of cold 100% ethanol.
 * 3) Place in -80°C freezer for 30 minutes of in an ethanol/dry ice bath for 5 minutes.
 * 4) Spin at 15,000 rpm in microcentrifuge for 30 minutes at 4°C.
 * 5) Rinse with cold 70% ethanol.
 * 6) Spin at 15,000 rpm in microcentrifuge for 10 minutes at 4°C.
 * 7) Remove supernatant, taking care of pellet, and dry fro 1 hour at 37°C.
 * 8) Resuspend in appropriate volume of buffer.