IGEM:Peking/2007/Switch-Notebook/2007-8-16

Double digested with XhoI/SalI
10uL    Plasmid 2uL    10*H buffer 0.25uL XhoI 0.25uL SalI 7.5uL  ddH2O -- 20uL    total

Run electrophoresis and send the correct plasmid for sequencing
SulA, lac, Rec, SS3, SD2 are sent.

SD1 are found wrong.