Recipe for 1X PCR Buffer

2.5ml   10X buffer (supplied by manufacture) Mg free 2.5ml   25mM Mg+² 50ul     dATP (100mM) 50ul     dCTP (100mM) 50ul     dGTP (100mM) 50ul     dTTP (100mM) 19.8ml Sterile dH2O 25ml- This will make 25ml. It can be scaled down to any amount you want.