User:Karmella Haynes/Notebook/Polycomb project/2010/07/06

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07/06/10

 * &#x2713; Transfection: KAH130, 131 into FTRx

Lipo transfection > Details

> Add Lipo to 250 μL Opti-MEM --> R.T/ 5 min. > Add DNA to 250 μL Opti-MEM > Add DNA mix to Lipo mix --> R.T./ 20 min. > Add 500 μL complexes to each well (4 ml med. each); Grow cells at 37&deg;C > Refresh medium after 5 hours (ab-free); add 1 μg/mL dox to dox+ samples > Next day: check transfection efficiency in dox+ samples

7/07/10 > ~50% RFP+ cells in dox+ samples > ~1% leaky expression in dox- cells


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