Timp:hydrogelProtocol

Hydrogel Protocol
Protocol for preparing PEGDA hydrogel, polymerizes under UV light.

Materials

 * PEGDA (Poly ethylene glycol diacrylate)
 * Acrylate-PEG-Acrylate-3400 (Laysan Bio, Inc.)or ACRL-3400 (NEKTAR 01010F12)
 * Argon gas
 * M9 Media (no antibiotics)
 * Photo Initiator (2-Hydroxy-2-methyl-propiophenone, 97%, Sigma Aldrich 405655)
 * 1.5ml Microcentrifuge Tubes (Fisher Scientific)

Making PEGDA

 * 1) Weigh out 100 mg PEGDA into microcentrifuge tubes.
 * 2) *Note: If storing, close container inside glove bag while flowing argon and store in freezer.
 * 3) Add 10% wt/vol of M9 media
 * 4) Spin at 10krpm for 20 minutes.
 * 5) Store in Fridge

Sample Preparation

 * 1) Add 100 μL of PEGDA solution to micro-centrifuge tube
 * 2) Add 0.3 μL of photo initiator and vortex mix for 1 minute
 * 3) Add sample and pipette up and down gently.