User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/04/28

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Incorporation of PCR BB product into backbone
Cut PCR results and Kan backbone seperately with EcoRI and PstI, then denatured restriction enzymes. Ligated DNA together with T4 DNA ligase. Knight:DNA_ligation_using_T4_DNA_ligase Transformed ligated product into E.coli by heatshock Transforming_chemically_competent_cells_%28Inoue%29 Plated transformants on amp+kan plate.


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