User:Fermenter User/Notebook/BROM B12

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM B12

Day 0 (Apr 21 Wed):
            4/21/2010 (14:00) Bring media to fermenter room            4/21/2010 (14:30) Assemble bioreactor 4/21/2010 (14:30) Add 5 ml of (Sterilized) Antifoam 4/21/2010 (15:30) Start Sterilization (Liq 45 min)

4/21/2010 (15:18) Cooling of bioreactor (Temp setup) 4/21/2010 (15:19) Start Fermentation Software for Fermenter #6 Record 0:00 4/21/2010 (15:20) Purge with 1.0 L/min O2                          4/21/2010 (15:22) Connect Acid (250 ml) and Base (400 ml), prime pumps 4/21/2010 (15:22) Start pH control 4/21/2010 (15:27) Calibrate dO2 probe with 350 rpm, Air purge for 30 min 4/21/2010 (16:00) Set dO2

---                4/21/2009 (21:00) Inoculation: (?? ml) Fermentation 0:00

Day 1 (Apr 22 Thu):
           4/22/2010 (9:30) Increased rpm to 500 F6: Acid (220 ml) Base (210 ml)

Day 2 (Apr 23 Fri):
            4/23/2010 (13:00) Sampling Fermentation 39:30 F6: Cell weight (172 mg/ml), Activity (96%, x100), Activity (72%, x1000)            4/23/2010 (10:30) Harvest F6: Acid (200 ml) Base (190 ml)


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