Gibson Assembly

Gibson Method, aka VLIC, aka one pot isothermal assembly
Original protocol from: this paper, doi:10.1038/nmeth.1318 (Shamelessly stolen and modified from Chris's Version).

Prepare a master mix, as detailed below. Store them in 15 ul aliquots at -20 °C. Then:
 * 1) Thaw a 15 μl assembly mixture aliquot and keep on ice until ready to be used.
 * 2) Add 5 μl of DNA to be assembled to the master mixture. The DNA should be in equimolar amounts. Use 10-100 ng of each ~6 kb DNA fragment. For larger DNA segments, increasingly proportionate amounts of DNA should be added (e.g. 250 ng of each 150 kb DNA segment).
 * 3) Incubate at 50 °C for 15 to 60 min (60 min is optimal).
 * 4) Transform as usual