User:Anthony Salvagno/Notebook/Research/2009/09/28/Digesting Anchor product

On Friday, Koch found my old PCR anchor product from Osley lab and we are going to use that until we get some better results with our own thermal cycler.

Protocol
I remember from back in the day BstXI wasn't the most efficient cutter, so I will digest for a long time and follow these results: BstXI Digestion and PCR Results. I might use a little more DNA this time because I don't know what my yield was back then.

I will run this reaction for 4 hours total. Tube 2 will come out after 2.

Gel Extraction
I want to do PCR cleanup, or maybe S4000 columns (but I don't think we have these), but I think that's what I did last time and maybe it didn't work. In the meantime I will do gel extraction, but I will see about other ways.