User:Fermenter User/Notebook/BROM E2

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM E2

Day 0 (Mar 16 Mon):
            3/16/2009 12:15 Bring media to fermenter room            3/16/2009 12:15 Assemble bioreactor 3/16/2009 12:20 Add 5 ml of Antifoam 3/16/2009 12:20 Adjust pH to 7.0            3/16/2009 12:30 Start Sterilization (Liq 45 min) 3/16/2009 14:03 Cooling of bioreactor (Temp setup) 3/16/2009 14:03 Purge with 0.5 L/min O2                          3/16/2009 14:04 Start Fermentation Software for Fermenter #6 Record 0:00 3/16/2009 14:20 Calibrate dO2 probe with 300 rpm, O2 purge for 30 min 3/16/2009 14:20 Connect Acid (500 ml) and Base (400 ml), prime pumps 3/16/2009 14:20 Start pH control 3/16/2009 14:25 Set dO2 3/16/2009 14:30 Completion of bioreactor set up: Ready for inoculation ---                3/16/2009 14:40 Inoculation: (?? ml) Fermentation 0:00

Day 1 (Mar 17 Tue):
           3/17/2009 (07:00) F6: Acid (480 ml) Base (320 ml)            3/17/2009 (07:30) Increase rpm to 600 Bioreactor picture taken             3/17/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)             3/17/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)             3/17/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)             3/17/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)

Day 2 (Mar 18 Wed):
           3/18/2009 (07:00) F6: Acid (470 ml) Base (300 ml) <Jadwiga>            3/18/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000) <Jadwiga>            3/18/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000) <Sung-Hye/Jadwiga>   3/18/2009 (16:00) Fermentation 00:00 Havest and Finish Fermentation F6: Acid (460 ml) Base (300 ml)


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