IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/19

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Sequencing Results

 * pBAD Strong, pBAD Weak1, pBAD Weak2, J23100+RBS, RBS-RFP-TERM miniprepped plasmids were sent to NAPS for sequencing using the VF2 and VR primers

pBAD Strong

 * Complete consensus for the pBAD sequence, all desired mutations are present
 * There is a mutation in the Biobrick prefix in the EcoRI restriction site. Both primer sequences show the same mutation

pBAD Strong1

 * Correct mutations are present
 * There is a mutation in the Biobrick prefix in the EcoRI restriction site. Both primer sequences show the same mutation
 * There is a significant number of unknown nucleotides in the sequence

pBAD Strong2

 * Correct mutations are present

J23100+RBS

 * Sequence failed - no signal

RBS-RFP-GFP

 * Weak signal
 * Sequence is correct


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