IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/14

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Team Flavor

 * Miraculin and brazzein constructs due to arrive on Wednesday from Mr. Gene

BioBrick Transformation
BioBrick parts from the 2010 iGEM kit were transformed and grown in highly competent TURBO bacteria. BBa_J45700 - entire pathway, Ampicillin BBa_J45004 - BSMT1 only, Ampicillin (Not in 2010 BB Kit: BBa_J45017 - PchB, PchA)
 * Wintergreen Scent Pathway:

BBa_J45250 - ATF3 + Promoter, Ampicillin? BBa_J45014 - ATF3 only, Ampicillin (Not in BB 2010 Kit: BBa_J45400 - BAT2 and THI3)
 * Banana Scent Pathway:

Team Fence
LacI Transformation

Performed bacterial transformation according to Silver:_Bacterial_Transformation, however we used 1μL in step 3, and used entire tube of TOP10 competent cells instead of only 10-15μL.

Transformed the following, each into its own tube of TOP10 E.Coli:

1) LacI with a rapid degredation tail attached, biobrick BBa_C0012, located on plate 1, well 2O, plasmid pSB1A2

2) LacI wildtype, biobrick BBa_I732100, located on plate 2, well 10E, plasmid pSB1A3

Streaked 100μL each of 1 and 2 onto separate LB + Amp dishes, along with a control dish of 100μL untransformed TOP10 E.Coli cells.

Plates incubated overnight, colonies observed in all plates except the control.