Cumbers:protocols/picogreen quantIT

1  2   3         4   A  10  10  1C        1S B 8   8   1C(1:10)  1S(1:10) C 6   6   1C(1:10)  1S(1:10) D 4   4   1C(1:10)  1S(1:10) E 2   2   1C(1:100) 1S(1:100) F 1   1   1C(1:100) 1S(1:100) G 0.5 0.5 1C(1:100) 1S(1:100) H 0   0   TE        TE
 * Follow the Quant-it protocol for DNA (and RNA) quantification.
 * Make up standard measurements along with your samples.
 * If unsure of your scale (if you have a lot or a little) then do 3 concentrations of your sample to check, one at 100% concentration, one at 1:10 other at 1:100
 * First you are making a 1:10 (add 1ul DNA to 9ul TE to make 1:10), then you are taking 1 uL of this 1:10 solution and adding it to 99 uL. So that is 1:100 of the 1:10 so the solution where you took 1 uL of this 1:10 solution and added to 99ul TE is 1:1000 of the original DNA concentration (1:10 * 1:100).
 * Do 2 of TE or whatever you are resuspending in to subtract this background from your results.
 * set up the plate reader and computer.
 * Add the fluorescent reagent (make up enough: 100 assays = 20 ml HS buffer) - 200ul added to each plate, make sure you have enough and careful when pipetting, keep track of which hole you want to add it to.
 * read the plate