Buckner Lab:Notebook/Summer 2010/2010/05/18

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Plasmids, Stocks
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Plasmid Isolation and Liquid Media
Objectives:
 * Analyze bacterial cultures inoculated 5/17
 * Prepare LB Broth medium for the Synthetic Biology Lab
 * Make a ampicillin stock for the SynBio Lab.
 * Make overnight cultures

Bacteria Results
MC= "multiple colonies present on agar"

LB Broth and Ampicillin Stock
-Made 300mL of LB broth media. Autoclaved at 9:47am -Poured broth into 6, 50mL portions. Stored in SynBio Lab at room temp -Started to make 10mL Amp (50mg/mL concentration) but spilled some during sterilization. Ended making 6mL of Amp stock and separating into 7 x's 700μL portions. Placed sterilized Amp in bullet box in Buckner freezer.

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