Jessica Karen Wong/Notebook/2007-7-12

Sequencing

 * Minipreped overnight cultures of E0240-3K3, I2057-3K3, T9002-3K3, T9002-1AK3 for sequencing
 * Sequencing I2057-3K3 w/ VF and VR
 * E0240-3K3 with E0040F and E0040R
 * T9002-3K3 with E0040F and R
 * T9002-1AK3 with F2620F and R (aka c62VF and c62VR)
 * Made overnights of E0240-1AK3 and T9002-3K3 (to look for F2620) to sequence tomorrow

PCRs

 * Ran gel of overnight I2055 col PCR and E0240 BB pcr
 * Nothing was right
 * Redid E0240 BB PCR with Vent and a 3x dilution of DNA

Ordered New Primers
T9002


 * F2620-F    8mer mfe1 TCCCTATCAGTGATAGAGATTGACATC 53.9
 * CTTAGTAG CAATTG TCCCTATCAGTGATAGAGATTGACATC


 * F2620-R    8mer xba1 not1 eco1 TTTATTCGACTATAACAAACCATTTTC 51.9
 * TCAGCGAT CTCTAGAAGCGGCCGCGAATTC TTTATTCGACTATAACAAACCATTTTC


 * E0240-F    8mer spe1 NOT1 pst1 TCACACAGGAAAGTACTAGATGCG 53.1
 * CTTAGTAG tactagtagcggccgctgcag TCACACAGGAAAGTACTAGATGCG


 * E0240-R    8mer nsi1 GTGGGCCTTTCTGCGTTTATA 53.6
 * TCAGCGAT ATGCAT GTGGGCCTTTCTGCGTTTATA

I2055/I2056


 * I2055-F 8mer Xba1 ATGCGTAAAGGAGAAGAACTTTTC 52.4
 * CTTAGTAG TCTAGA ATGCGTAAAGGAGAAGAACTTTTC


 * I2055-R 8mer xba1 NOT1 ecor1 GTGCTCAGTATCTCTATCACTGATAGG 52.0
 * TCAGCGAT CTCTAGAAGCGGCCGCGAATTC GTGCTCAGTATCTCTATCACTGATAGG


 * I2056-F 8mer Xba1 ATGGCTTCCTCCGAAGACG 54.0
 * CTTAGTAG TCTAGA ATGGCTTCCTCCGAAGACG


 * I2056-R same as I2055-R