IGEM:MIT/2007/Notebook/2007-7-20

Agenda

 * Dilute liquid cultures of Colony PCR 3A(F+B+CPX) #5,6,8
 * Check antibiotic resistance screening plates first
 * Some for Miniprep
 * Some of Miniprep for sequencing
 * Some for glycerol stock
 * Some for Polystyrene-binding Assay
 * Check about ordering biobrick primers
 * Streak DH5a for mercury assay
 * Transformation of TK Chemically Competent Cells (BH, SK, JH)
 * 1 µl of BioBrick DNA (I13500)
 * 1 hour incubation in 37 degree room
 * Spread 150 µl on A+ plate --> put in warm room at 2.30 PM ("iGEM 2007 7/20/07 TK Cells Transf. I13500")
 * Will pick colonies to make liquid cultures