IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/07/Min Lin

01:52 GEL to assess the digestion CIP the 6 rbs vectors. Add to the digestion product 5ul CIP Buffer and 1ul CIAP

Run a GEL to recycle the insert of tetR. The Hole is too large, the band is missing. (Never use the largest Cone for 20ul recycle any more)

03:24 Digest the tetR plasmid again.