Jasieniuk:Notebook/Apomixis in hybrid Rubus/2010/10/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Apomixis in hybrid Rubus
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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ABI runs

 * Made dilution plate for panel 2 (RUB101026A)
 * Made formamide plates:
 * Leftover formamide + GS500 mix from 10/21 wento into RUB101026B wells A1-D4
 * We ran out of GS500, so I added a total of 13.5 ul GS500 to 1500 ul Hi-Di Formamide
 * 19 ul formamide mix + 1 ul diluted PCR product
 * Spun down and denatured formamide plates
 * Ran all of plate RUB101026B on ABI3100 today: Runs 6292-6297
 * Plate RUB101026A1 = columns 1-4 of RUB101026A
 * RUB101026A1 columns 1-2 = RUB101012 columns 11-12 plus fresh RUB126 reactions in 6 of the wells = Run 6290
 * RUB101026A1 columns 3-4 = Run 6291


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