User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/21/D2O experiments

Stock solutions
Yesterday and today I prepared stock solutions for heavy hydrogen PEM and deuterium depleted PEM. The procedure/recipe for both of these are below.

Heavy Hydrogen PEM
I am going to call this H-PEM to indicate that it is made up of heavy hydrogen water or, D2O. I didn't call it D-PEM for reasons below.
 * Preparation of a 10x solution of H-PEM
 * 800 mM PIPES
 * 10 mM EGTA
 * 10 mM MgCl2
 * 1.3544 M NaOH
 * pH 7.30
 * Filtered through a 0.2 um filter and aliquoted in 2 mL screw top cryogenic vials in the 4C fridge.

Deuterium depleted PEM
This is called DD-PEM.
 * 800 mM PIPES
 * 10 mM EGTA
 * 10 mM MgCl2
 * 1.2265 M NaOH
 * pH 6.89
 * Filtered through a 0.2 um filter and aliquoted in 2 mL screw top cryogenic vials in the 4C fridge.