TSS

Uses
TSS buffer is used to make chemically competent cells.

Preparation
To make 50 mL:
 * 5g PEG 8000
 * 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
 * 2.5 mL DMSO
 * Add LB to 50 mL

Filter sterilize (0.22 &mu;m filter)

Storage
Store at 4˚C or -20˚C.