20.109(F09):System engineering research article guidelines

Day 1
In advance of Module: In advance of Day 1: On lab day:
 * streak out NB334 onto LB+Amp+Cam+Kan
 * streak out NB188 onto LB+Amp+Kan
 * streak out NB5 on LB+Amp
 * Prepare 50 ml bottles of indicator media but don't add H2O
 * Grow ONs of NB5 in LB+Amp
 * In AM subculture NB5, 1 ml/20ml LB+Amp on RT shaker. Induce with IPTG at start of lab period.