Template:SBB10 ConstructionFiles KaiM sbb16

sbb16: phiC31 attP

Wobble KRM003/KRM004           (115bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144        (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb16     {phiC31 attP} KRM003  Forward construction of phiC31 attP basic part ccataGAATTCatgAGATCTagaagcggttttcgggagtagtgccccaactggggtaacctttgag KRM004  Reverse construction of phiC31 attP basic part ctgatGGATCCgtgtcatgtcggcgaccctacgcccccaactgagagaactcaaaggttaccccagttg



JCA Notes

 * correct
 * Entry modified on clotho, seq included eco, bam, and tails