IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-9-26

To do

 * 50mL cultures of:
 * Lac+RBS+KaiA (Nick's A09): AMP Resistance
 * Lac+RBS+KaiB (Nick's B11): KAN Resistance
 * Lac+RBS+KaiC (Peng's M2-7): KAN Resistance
 * B0034 (from frozen): AMP Resistance
 * Send B11 and A09 for sequencing
 * Glycerol stock B11 and A09
 * Save some of M2-7 for growth testing
 * Midiprep the above

Determination of what needs yet to be created

 * We need to make to complete stage 1:
 * RBS + KaiB
 * RBS + KaiC
 * KaiA (biobricks registry)
 * KaiB (biobricks registry)
 * KaiC (biobricks registry)


 * We need [semilarge] concentrated amounts of:
 * Lac+RBS+KaiA (created by Nick)
 * Lac+RBS+KaiB (created by Nick)
 * Lac+RBS+KaiC (created by Peng, verified)
 * B0034 (which contains just RBS)
 * KaiA
 * Have a lot of
 * KaiB
 * Have a lot of
 * KaiC
 * Have a lot of
 * J04500 (for the biobricks registry pieces)
 * Have a lot of

Inventory

 * Box labeled with red type, "Nick's Miniprepped Lac+RBS+Kai from 9/4"
 * Nick, are there frozen stocks?
 * Box labeled with pink tape, "Post-Digested Midi/mini plasmid insert or backbone"
 * J04500 /SP from 8.29.06 (w/o EtBr)
 * KaiC /XP from 8.29.06 (w/0 EtBr)
 * Box with white tape, "Nick's KaiA/B/C+J04500 cell pellets from 9/1"
 * Box with orange tape, "Predigested mini/midi plasmid"
 * B0034 in limited amount, low concentration
 * J04500, a lot of midiprep. USE YELLOW TUBE FROM 8.22.06
 * KaiA, B, C: A lot from midiprep, labeled "Geneart KaiX"
 * Plates labeleed "very important"