User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/08/10

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Maize Endophyte Biofertilizers
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The Daily Grind

 * Autoclave 30 tubes and 30 lids on the p2 setting
 * Make 700mL of media comprised of 3.08g Minimal Organics Media, 14g agar, and 700mL dH20 > all adjusted to a pH of 6.0
 * Cool each tube on a slant of approximately 45 degrees
 * Being sterile, place 6 day old germinated seeds into the 30 tubes filled with the 20mL of now solidified media
 * Making sure a root from the seed is contacting the media, parafilm the top of the tube and make a 1cm slit in the parafilm


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