User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] HeLa lysis, Bradford & cAMP precipitation part I
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Protein work

 * HeLa (Passage 16) cell lysis as described 08April2010
 * 500 μL RIPA buffer for T25 flask (2 more T75 flasks were ordered)
 * Protein determination as described 21June2010
 * HeLa
 * HEK293
 * hTERT ASM Samples
 * cAMP precipitation
 * Everything was put on beads corrected to the lowest concentration, to at least get similar relative amounts (see appendix)
 * Beads were incubate ON @ 4 °C

Attachment
   [[Media:Bradford_28062010.xls| Bradford results]]


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