Mimulus:Seed Sterilization

Supplies

 * 70% Ethanol
 * 20% Bleach
 * Sterile Water
 * Seeds

Methods

 * 1) Put seeds in an Eppendorf tube
 * 2) Add 200uL of 70% EtOH to tube
 * 3) Immediately add 600 uL of Water. Do only 2 tubes at a time, because the alcohol treatment is harsh and might affect germination if is left on seeds too long
 * 4) Immediately suck off 600 uL of supernatant
 * 5) Repeat steps 3 and 4 four times with water, not ethanol, to wash
 * 6) Set aside tubes and repeat steps 1 to 5 for all the remaining tubes
 * 7) Add 200 uL of 20% bleach to each tube
 * 8) Immediatly remove bleach solution
 * 9) Add 600 uL of water
 * 10) Suck off 600 uL of the supernatant
 * 11) Repeat steps 9 and 10 four or five times to wash