User:Anthony Salvagno/Notebook/Research/2009/02/04/Spheroplasting Buffer

This is a solution that aids in the process that will chew up the cell walls and create spherical yeast cells instead of the weird blobs that they are... fatties!

=Prep= To make in general, need:
 * 1.2M Sorbitol
 * 0.1M EDTA
 * .05M (50mM) Tris-Cl pH 8.0

We made 500mL:
 * 1) Sorbitol: 182.17 g/mol -> 109.3 g for 500mL
 * 2) EDTA: 372.24 g/mol -> 18.61 g for 500mL
 * 3) Tris: 25mL (Kelly had liquid stock so no mixing necessary) (Steve Koch:so, interpreting that he had 1 M stock, and you added water to 500 mL after that?Anthony Salvagno:Yea I think so.)