Image:DSCF0997.JPG

This picture is of the positive control with the +Fluo-3, AM. The magnification is 100X on mouse embryonic stem cells under FITC lighting. The intent of the procedure was to see the effect of histamine on the flourescence of these cells. Histamine would increase Ca2+ by opening ion channels in the cells and allowing Ca2+ to rush in due to a concentration gradient. This positive control is without the histamine, but flourescence can still be seen due to the free Ca2+ in the cells normally.