IGEM:MIT/2006/Notebook/2006-8-21

Ligations
Ligate osmYES with XP cut:


 * 1) Q99ABC
 * 2) Q119ABC
 * 3) Q199ABC
 * 4) Q219ABC
 * 5) QE0840ABC

(after running a gel to make sure inverter Q044000 is around)

Mutagenesis
Did mutagenesis on 30-BAT2-30-THI3 to get rid of site in THI3 and on pchBA-15

Transformations
Transform ligations and mutagenesis products