Jessica Karen Wong/Notebook/2007-6-28

To Do

 * Redo PCR's of E0240, I2055, and colony
 * Re-digest B0032
 * Re-transform B0032 from Igem Distribution Kit
 * Replate yesterdays' transformations
 * Analyze the PCR gels
 * ligate and transform new devices?

PCR

 * Diluted dNTP's to 2.5uM
 * Did a 10 ul colony PCR on the 7 colonies of blue C
 * Did a 100ul preparatory PCR of E0240 at 49.5
 * PCR'ed I2055 on a gradient (12 10ul)
 * Ran a gel - Top is I2055, bottom Left is colony and bottom Right is E0240
 * E0240 has a very bright band at the right size
 * Colony PCR's didn't show
 * I2055 looks hazy but there might be something there?
 * PCR cleaned E0240

B0032

 * Digested the B0032 stock on 1A3 (in the measurement kit box) with EcoR1 and Spe1
 * DNA concentration was very low 45.2 ng/ul - hopfully that's ok
 * In case that batch is bad we transformed the Igem Distribution of B0032 to grow it up

RBS Tester

 * Yesterday's overnight transformation plates had no growth yet again
 * Spun down and replated the rest of yesterday's transformation
 * Ligated B0032-J04650-1AT3 and B-J-1AC3 from today's digest of B0032
 * Transformed today's ligation and plated for overnight