IGEM:PennState/Labbook/NoahJohnson/2007-9-16

September 16, 2007


 * Started overnight ligation with crp* and psB1A2 E+S restrictions
 * Used a 6uL:2uL ins:vect ration
 * Used PCR tubes and 7.86pH dH20
 * Left 2 ligations in the 16C fridge and 2 ligations on the benchtop in ice to melt overnight with a negative for each
 * Innoculated starter culture of DH5a for competent cell prep tomorrow morning

6:30-8