User:Meng Xiao He/Notebook/fall08/2008/11/06

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pir-116 maintains plasmids at high copy

 * higher than glycerol stock strain, 300-400 ng/ul

Distinguishing digests

 * TOPO (3.5kb): EcoRI+PstI
 * Duo: 1.2 kb, 300bp
 * cco: 1.7 kb
 * cbb3: 400, 830, 300
 * pER21+flanks (and flankless as ctrl):
 * EcoRI + PstI>column>SmaI at 25

Gel 1: pER21+flanks, EP>SmaI digest

 * 1-3: A1-3
 * 100 bp ladder
 * 5-7: B1-3
 * pER21
 * 1kb ladder
 * 10-12: C1-3

B2 is cbb3 candidate & C1 is cco candidate

Gel 2

 * pER21: EP only
 * 1kb ladder
 * 3-5: TOPO A, B, C
 * 100 bp ladder
 * A1-3 EP only
 * B1-3 EP only


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