User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/07/28

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=Wet Lab: OmpF/C promoters characterization=

Electrophoresis Gel OmpF PCR_RTTH
In order to probe the PCRs reactions an electroforesis gel was made. This was loadded as follows:
 * Ladder
 * positive control B3K3
 * B3K3 OmpF
 * positive control B1T3
 * B1T3 OmpF



There were not positive results for none of the reactions, even for the B1T3 OmpF two bands were obtained.

OmpF PCR_TaqDNApol
A second set of PCR rections was made, this time with the TaqDNApol enzyme.



PCR's Programme

 * 94ºC, 5:00


 * 35x
 * 94ºC, 0:45
 * 55ºC, 0:45
 * 72ºC, 3:00


 * 72ºC, 10:00 <<
 * 4ºC, ...

The temperature for extension (<<) was change. The one used for the RTTH PCR from 27/07/10 was to high, this cloud be a possible explanation why the reaction was unsuccesful.


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