IGEM:IMPERIAL/2007/Projects/pH Buffering Machine/Design

=pH Buffering Machine: Design=

 Introduction Specifications Design Modelling Implementation Testing/Validation Notes References </ul> <br style="clear:both">

List of Parts Required

 * From the registry
 * BBa_J23100: Constitutive promoter
 * BBa_C0012: Coding region for LacI
 * BBa_R0010: pLac promoter – activated by IPTG
 * BBa_E0040: Coding region for GFP
 * BBa_J06504: Coding region for RFP
 * BBa_R0080: pAra promoter – activated by AraC
 * BBa_C0040: Coding region for TetR
 * BBa_R0040: pTet promoter
 * BBa_B0034: RBS
 * BBa_B0015: Double terminator
 * Not yet in the registry
 * NC_000913: Coding region for CadC
 * M67452: pCad promoter
 * Coding region for HrpR
 * Coding region for HrpS
 * PhrpL promoter
 * NC_002655: Coding region for urease
 * NC_007946: Coding region for lactate dehydrogenase

Chassis Selection

 * E.coli K12 (strain K-12; substrain MG1655]