Paulsson:Pmalkus thoughts

Improved response time
Estimate GFP-ssrA that can be degraded by ClpXP/ClpAP in molecules / min Compare to reasonable detection limit in FACS


 * Use pBAD UBP1 to modulate degradation rate

How to measure degradation rate below detection level? - can't be fluorescence!!!
 * Western or pulse/chase IP

Do the same for PEST / proteasome in S.cerevisiae

Critical parameters for plasmid counting
Doubling time Plasmid loss rate (when using ts plasmid)

FP reporter
 * synthesis rate
 * degradation rate
 * maturation rate

How do these vary with temperature, growth media...