User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/31

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Goals

 * 1) Perform ECL (Enhanced Chemiluminescence) Detection of iNOS/NOS from the Western Transfer blot.
 * 2) Perform a Nitric Oxide Assay on the LPS +/- IFNg stimulated culture supernatants

Part 1: ECL Detection of iNOS/NOS from the Western Transfer Blot

 * Procedure followed as in lab manual pg 130.

Standard Curve Values and Calculations

 * £ Used to calculate standard curve more accurately

Sample Concentrations

 * *Note: Normalized to "0" because it doesn't make sense to have negative concentrations.
 * **Note: 100X absorbance was used to obtain a more accurate equation for the standard curve.

Calculations File
Calculations can be found in [[Media:090331_NO_Assay.xlsx|this]] document.


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