Drummond:Confocal

Starting up the system

 * 1) Turn on mercury lamp (after 1/2 hour, turn down to 50% power)
 * 2) Turn on main power
 * 3) Turn on computer
 * 4) Turn on lasers
 * 5) Open LSM510 software
 * 6) Set laser output:
 * 7) * Helium-neon 1: 50% (543nm line suitable for RFP excitation)
 * 8) * Argon: 5% (458nm line suitable for TFP excitation)

Slide preparation
(cf. cell preparation)
 * Prepare 15 mins in advance to allow drying
 * 15uL droplet
 * No. 1 1/2 cover slip
 * Push down gently on cover slip to squish out extra liquid and any air bubbles. Cover slip should not float!