IGEM:Stanford/2009/Project Homeostasis/Experimental Archives

/*- General */ body { background: black; color: black; }

System Overviews

 * [[Media:Group_2_rewrite.doc|Detailed Summary of Homeostasis Project]]


 * Link to googledocs image: Device Level Diagram


 * [[Media:SystemsImage.jpg| Image of System and Genes Created by Mary and Leon]]


 * [[Media:SystemsImage_v2.jpg| Image of System and Genes Created by Mary and Leon_version II]]


 * [[Media:Construction_Plan.doc |Detailed Construction Plan]]


 * [[Media:Construction Plan 2.doc |Detailed Construction Plan (Updated)]]


 * [[Media:Plasmids.pdf |PDF of 4 Plasmid Designs]]


 * [[Media:Plasmid_Pictures.pdf |PDF of Overview of Plasmids and System]]


 * [[Media:Plasmids_Pres.ppt |Pwpt of Combined Plasmids and Systems PDFs]]

Inflammation Device
Goal: To design the device that monitors and responds to inflammation.
 * a. Input: a promoter that monitors inflammation by detecting levels of Th17-derived markers.
 * b. Output: a gene that responds to inflammation by inhibiting the Th17 response and enhancing the Treg lineage.
 * [[Image:Inflam1.png|thumb|center| Anti-Inflammatory Device (Developed So Far)]]

Immunosuppression Device
Goal: To design the device that monitors and responds to immunosuppression.
 * c. Input: a promoter that monitors immunosuppression by detecting levels of Treg-derived markers.
 * d. Output: a gene that responds to Treg-driven immunosuppression by inhibiting the Treg response and enhancing the Th17 lineage.
 * [[Image:Immuno2.png|thumb|center| Immunosuppression Device (Developed So Far)]]

Discussion

 * E.Coli Genome: http://ecocyc.org/

Paper Pages

 * PLoS. Role of IL-6 in mice.


 * Review of SoxR/SoxS


 * E.Coli Export

Primer Design

 * Guidelines and Helpful Websites
 * Original Primers

Gene Sequences and Orders

 * Link to googledocs: Sources of Genes and Sequences


 * [[Media:Sources_of_Genes_and_Sequences1.doc| Sources of Genes and Sequences]] (googledocs is better)


 * [[Media:InternalRESites.doc|Gene Sequences w/ Highlighted RE Sites Document]]


 * [[Media:AssembledPiecesSequencesDeNovo.doc| Sequences to be De Novo Synthesized]]


 * [[Media:Assembled_Pieces_Sequences_De_Novo.doc | Stanford IGEM's 4 de novo synthesized sequences]]

Trp Repressor Analogue

 * 5-methyltryptophan
 * Trp Notes From Yanofsky Meeting 1

Parts

 * Link to Spreadsheet on googledocs to Edit: BioBrick parts and Assay Ideas


 * Tentative List of Parts for Registry [[Image:Parts.doc]]