User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/10

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12/10/09

 * &#x2713; Minipreps: KAH109-116 (2 each, 16 total)
 * &#x2713; Lab meeting at 4 pm! (Kevin and Karmella)
 * &#x2713; Assemblies: KAH126-133; KAH134
 * &#x2713; Cultures: KAH120-125 (3 each, 18 total)

Minipreps > Check with E/P digests

--> 37°C/ ~15 min.

Assemblies
 * 1) KAH126: CMVTetO2-K(KAH3)/(S/P)/3951 &#x2713; + (1) KAH109/(X/P)/1123
 * 2) KAH127: " + (2) KAH110/(X/P)/1123
 * 3) KAH128: " + (3) KAH111/(X/P)/1123
 * 4) KAH129: " + (4) KAH112/(X/P)/1123
 * 5) KAH130: " + (5) KAH113/(X/P)/2107
 * 6) KAH131: " + (6) KAH114/(X/P)/2110
 * 7) KAH132: " + (7) KAH115/(X/P)/931
 * 8) KAH133: " + (8) KAH116/(X/P)/775
 * 9) KAH134: (10) Streptavidin tag(Joe)/(S/P)~3200 + (9) KAH67/(X/P)/1353

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations


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