User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/12

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AMPure Cleaned 454 Alien Gene Products
1) Combined 5.0ul of each culture #1-12 = 8 tubes 2) Cleaned with AMPure beads http://www.beckmangenomics.com/products/dna_purification_and_cleanup/agencourt_ampure_xp.html 3) Eluted with 40.0ul EB Buffer from Qiagen Extraction Kit.

Loaded 2.0ul on Gel



Yeast Culture Prep for Long Term Freezer Storage
From Revived Yeast Culture NCYC2722

1) Overnight culture on agar plate (or a few day until lawn is of sufficient size)

Streaked out 50.0ul of growing culture onto a glucose+yeast extract plate

S. pombe - failed to grow - redo S. cerevisiae - good lawn

2) Prepare freezing media = 20% gycerol in sterile YM media. Aliquot into small freezer tube (1000ul).

3) Collect lawn with sterile toothpick and swirl in freezing media

4) Slow freeze in -30C