User:Kristina M Holton/Ovarian Notebook 2007 7 16

Bioanalyzer for Ambion & Qiagen FFPE Kits

First chip did not run -> voltage problem Make sure: no bubbles, liquid at bottom of wells Suggestion: practice loading technique on sample known to contain purified small RNAs; practice with another chip
 * Test plates OK
 * V=IR; V=Q/(eA/d) (V=Q/C) so...Q & I are fine, problem with loading gel (dialectric)
 * Chip pressurizing syringe changed
 * Small RNA assay very temperamental according to Agilent