IGEM:Stanford/2009/Notebook/Personal Notebook/2009/08/18

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] Suzie's iGEM Project
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Daily Work
Goals

Project / Accomplishments
 * [[Media:Grad_Pres.ppt |Pwpt given at Grad Meeting for Diagrams]]


 * Revised Construction Diagrams from Grad Meeting
 * [[Media:Grad_Pres1.2.pdf |PDF of new construction diagrams]]


 * [[Media:IBD_Clinician_Update.ppt |Pwpt of Update from Clinician Dr. Shah]]


 * Wrote up detailed protocol for PCR w/ new primers
 * [[Media:PCR_Protocol_Temp.doc |PCR Protocol]]

Problems

Alternative Solutions

Events

 * 9am meeting w/ Grad Student
 * Anusuya w/ IBD and Immune System
 * Not so much Immunology....
 * Emphasize work w/ Il-10 treatment= PRECEDENT
 * Need slide w/ differentiation
 * Too many slides
 * Symptoms- mention somewhere else but don't necessarily need its own slide
 * Is video necessary?
 * Emphasize why our therapy is BETTER
 * Awesomeness on 1 slide - update advantages slide to compare to current therapy
 * Table? Larger font?
 * Answer question more clearly about why we need device 2
 * Suzie's Slides w/ Constructions
 * Slide 1 - colored lines and provide key
 * Slide 2 - mention vBOL
 * Slide 3 - ask about sequences leading to protein- is it correct? Add arrows
 * Graph Larger
 * Slide 4- Easiest to understand and just label important parts..weak constitutive beta carotene?? OR not?
 * Slide 5 - metadata?
 * Slie 6- rest.... revise for device 2... const export system?
 * Mark and lab update...
 * Cultures of parts... mini-prep and pcr w/ new primers...


 * 5:30 meeting w/ Clinician
 * Update From Meeting with IBD Specialist Dr. Shamita Shah
 * Good News
 * Fully supportive of the concept
 * Understands importance of and our reasoning behind both devices
 * Supports probiotic research
 * Enthusiastic for future implications of this device as a “treatment/diagnostic tool”
 * Willing to share her own slides on IBD and give 20 presentation on her current knowledge
 * She has great statistics and can help us frame our device to tailor to a specific target audience
 * Next Steps
 * Decide what “phase” of IBD we want to target
 * Early diagnostic… difficult to distinguish between UC and Crohn’s, but certainly useful
 * Complementary treatment: use as another “tool” for clinicians
 * Post-treatment diagnostic to prevent recurrence…
 * Dr. Shah sees this as the most promising and necessary right now w/ regards to treatment
 * No previous probiotic research in this field
 * Many cases lead to recurrence- and patients need annual colonic scopes
 * Meet w/ Dr. Shah again early September
 * Challenges
 * We need numbers…
 * The “ratio” needs to be known or better understood and Dr. Shah doesn’t collect these numbers
 * When/how will the device be “triggered” with regards to these numbers?
 * Switch bacterial strain to be more sustainable in the small intestine?

Weekly Goals

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