User talk:Federico Castro M

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This is a place for you to comment, ask or discuss anything about my work.

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Jason R. Kelly 02:07, 17 January 2008 (CST):

Hi Frederico,

I'm a grad student in drew endy's lab, was taking a look at your repressilator project. You might consider using a different CFP then the one contained in E0422. Unfortunately, the ssrA tag on E0022 is LVA and that's stronger than the tag used by Elowitz. It's so strong that you basically get no detectable signal since the FPs get degraded so quickly (even when behind a very strong promoter). I'd recommend going with the same tag as elowitz if you're going to give this a try, or at least a weaker tag than LVA.

Thanks for posting the project up. Also, I saw you're keeping a lab notebook on OWW -- we're rolling out a new lab notebook tool that you might want to try out. you can sign up for the SC mailing list to keep tabs on it: http://mailman.mit.edu/mailman/listinfo/oww-sc

We'll also post something on the SC blog when it comes out. http://blog.openwetware.org/sc/

good luck, jason