IGEM:UC Berkeley/2006/Gel extraction


 * 1) cut out band minimizing extra gel matter.
 * 2) put in ependorf tube and add 600uL of QG.
 * 3) vortex until the gel has dissovled.
 * 4) add 100uL of isopropanol alcohol.
 * mix
 * 1) pour into the minelute column. this has a dark purple band around the resin which distinguishes it from the miniprep columns.
 * 2) spin through. and disgard waste.
 * 3) the book says to run more QG through but i dont because it doesnt do anything.
 * 4) wash with 750uL PE buffer.
 * 5) spin for two minutes to dry.
 * 6) elute with 2mM Tris buffer.