IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/22

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Stock ECE112, 166, 153
- Plasmid miniprep (2 tubes of ECE112, 2 tubes of ECE166 and 1 tube of ECE153)

- Nanodrop

Transformation of Bacillus

 * For strain IA751 : transform with ECE166, 171, 153 and 112


 * For strain IA771 : transform with ECE166, 171


 * for glycerol stocks (one with 100μL of medium B and glycerol, and one with only glycerol) : transform with ECE166

- We used the same protocol than before with a few changes :


 * add sterile 50% glucose in medium A
 * don't keep samples after using them for spectrophotometer
 * spin each tubes and keep only 100μL of medium A (to concentrate cells) before adding DNA



- For glycerol stocks, resuspend cells into 100μL of medium B, add DNA and wait for 30min, end plate them