Template:SBB-Protocols Enz3

BglII Digest of EIPCR Products
'''For EIPCR products, you will only digest a portion of your purified PCR product. This procedure is almost identical to the digestion of a PCR product with EcoRI/BamHI, but you'll use BglII instead of EcoRI/BamHI.''' 8.5uL of eluted PCR product 1uL of NEB Buffer 2 1uL BglII
 * Set up the following reaction:
 * Incubate at 37 degrees on the thermocycler for 1hr
 * Run an agarose gel, cut out the band, and melt with 600uL ADB buffer at 55 degrees
 * Proceed with remainder of Zymo gel purification protocol