Duffy:LIC Cloning

Overview
Clone genes into pEU-E01-LIC1 protein expression vector using ligation independent cloning (LIC).

Materials
List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.


 * supply 1 (i.e. tubes of a certain size? spreaders?)
 * reagent 1
 * X &mu;L reagent 2
 * component A (reagent 2 is made up of multiple components)
 * component B
 * equipment 1
 * equipment 2

Procedure

 * 1) Step 1
 * 2) Step 2
 * 3) *Step 2 has some additional information that goes with it. i.e. Keep at 4&deg;C.
 * 4) Step 3
 * 5) Step 3 has multiple sub-steps within it.
 * 6) Enumerate each of those.

Contact

 * Who has experience with this protocol?

or instead, discuss this protocol.