Template:SBB10 ConstructionFiles CarolynK sbb24

Construction of O99 5'UTR Basic Part sbb24

PCR sbb24F and sbb24R on pEC52          	    (468 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144	           (EcoRI/BamHI, 2472+918, L) Product is pBca9523-sbb24 		    {O99 5'UTR} - sbb24F Forward oligo for cloning of sbb24  ccataGAATTCatgAGATCTacgattctgacgcattttttatg sbb24R Reverse oligo for cloning of sbb24  ctgatGGATCCacgtcaaaaaccagccagaactgcg



JCA Notes
PCR sbb24F and sbb24R on pEC52          	    (468 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144	           (EcoRI/BamHI, 2472+910, L) Product is pBca9523-sbb24 		    {O99 5'UTR} - sbb24F Forward oligo for cloning of sbb24  ccataGAATTCatgAGATCTacgattctgacgcattttttatg sbb24R Reverse oligo for cloning of sbb24  ctgatGGATCCacgtcaaaaaccagccagaactgcg
 * Vector sizes slightly off, but otherwise correct