Alm:Lab equipment

Alm:Internal

Hints from a technician in the Endy lab: Basic plasticware (gloves, pipette tips, eppendorf tubes) we get from [ISC Bioexpress]. They are by far the cheapest but to get those prices you have to deal with the company rep. who issues a price quote. USA Scientific (a sister company of Eppendorf) is also a good source for tubes, tips, and racks, and the lower prices are available through ecat3 on MIT's ecat. A lot of people simply use the VWR stockroom in [Building 56] and just go and buy what they need as they need it. You can also just start signing up for accounts with different companies online and start requesting their catalogs so you can get a feel for what they offer. With most companies you can also use Purchase Orders if you prefer or order through webpages with a credit card. Eric has an MIT credit card.

Often-ordered product numbers
S41873 Butane burner canisters (Replacement Fuel For S41872) (Fisher) ($7.85ea + 21.50 hazmat charge) Wall Lenk Vendor No.:65F

21008-959 Eppendorf Safe-Lock 1.5 mL Tubes Natural 022363204 Pack of 500 $34.27

9187-1000 Tough Tags for 1.5ml tubes 1.2x0.5" white (USA)

9185-0500 Tough Spots for 1.5ml tubes 0.5" diam. white (USA)

2344-1000 Tubular 4-way tube racks in set of 4 + tray (USA)

0497735 4 chisel tip dry erase markers (OD)

0254452 Uniball refills black (OD)

32916-536 Large Evolution One gloves, case 500 (VWR)

32916-500 M Diamond Grip, CS 10 boxes (VWR)

60819-761 14ml Falcon (352059) dual-position snap-cap polypropylene culture tubes, case 500 (VWR)

25384-094 case of 600 (25 sleeves of 24) stackable for automation 100x15mm Petri plates (VWR)

73520-774 Nunc large square plates DISH BIOASSAY LO PROFILE case 20 (VWR)

37000-700 galaxy micro centrifuge for spinning down tubes and strips $270 includes rotors/adaptors (VWR)

2004-00004 Silicone closures for flasks, 10 (Bellco Glass, Inc)

14220-010 Autoclave bags, clear with biohazard symbol (available without for $225.83), 12"x24" (slightly bigger?), 1.5 mil polypropylene Case of 1000 $243.64

95042-554 Autoclave bags, clear, 12"x24" 2mil thick polypropylene PK100 $33.05

WLS2493-A PK100 $19.95 VWR BAG AUTOCLAVE 12X24INPK100 Supplier: Tufpak (haven't tried these but they're cheaper)

Vendor numbers

 * Fisher 142706

Miscellaneous

 * Desk chairs: Office Depot (may be cheaper to go through regular site than the SAPwebpage)
 * Herman Miller through procurement office NE49 600 Tech Square 4th floor


 * Lab stools
 * VWR BioFit High Bench height (lab benches require chairs to go up to 29 inches)
 * VWR Urethane lab stool


 * Telephone [MIT's phone service page]
 * All requests for new telephone service or changes in existing service should go through your department's Administrative Officer (AO). Email customer service at telecom-csr@mit.edu or call 617-253-3670 for April-White Frager (Civil's rep).

Lab-wide Equipment

 * Streamline Vertical Laminar FLow Cabinet SCV4A2 2007 20789
 * Eppendorf PCR machine
 * Heat Blocks (dry baths)
 * Stir Plate / Hot Plate
 * Waterbath (s)
 * Incubators
 * [Torrey Pines] brand, models IN35 and IN45: 4C-70C, 27L or 55L, see [description]
 * -20C Freezer (double-size, used from Broad, Fisher Sci model)
 * 4C Refrigerator (glass front?) (VWR or used)
 * Benchtop Centrifuge for culture tubes
 * Microcentrifuge, Epp unrefrigerated
 * Nanodrop
 * Microbalance (enclosed, for up to __ g)
 * Balance <400g


 * Test tube roller
 * Pressure cooker/hot plate for low cost autoclaving or small autoclave on wheels like on 4th floor

Bench Top Equipment

 * Mini spin, VWR
 * Burners
 * Gas canisters
 * Butane torch and lighters
 * Vortexer
 * Pipette sets (2.5, 20, 100?, 200 and 1000 ul) find out what tips work, get samples)
 * Repeating pipet
 * Pipet man (motorized pipetter)
 * Thermometers (glass for liquids and infra-red for surface measurements)
 * Timer

Disposables/Plasticware
[ISC Bioexpress]


 * Pipette tips (with/without filter)
 * Gloves latex, nitrile
 * PCR tubes/plates
 * Eppendorf Tubes
 * 15 ml Conical Tubes
 * 50 ml conical tubes
 * 14 ml culture tubes with two-position caps
 * VWR brand lab markers
 * Lab Tape (Tape holder)
 * Kimwipes
 * Cryovials
 * Tough Spots
 * Razor Blades
 * Bottle top filters (Westnet)
 * Freezer boxes (-20 and -80)
 * Racks (plastic for various size tubes)
 * Petri Plates (BD/Falcon?)
 * Pasteur Pipettes (and bulbs)

Glassware/Pyrex
Westnet locally (best prices/service), direct from Corning, through Sigma, also maybe available used


 * Erlenmeyer flasks (baffled for cultures and 1 L for making chemical stocks)
 * Graduated Cylinders
 * Media Bottles (we order from 50 ml up to 1 L)
 * Beakers (maybe?)
 * Test tubes

Other items

 * Safety Glasses $7 at VWR
 * Lab coats
 * Test Tubes
 * plastic pipettes
 * Plastic Bins (greatly help with organization)

Chemicals
[Sigma/Aldrich] (bad service, $10 2-day shipping) or VWR
 * Media reagents
 * Antibiotics
 * Protoplast fusion ingredients: EDTA, sodium maleate, etc.
 * EtBr or SYBR Green/Gold/Safe

Enzymes

 * Basic set of restriction enzymes
 * PCR reagents: DNAPols, dNTPs
 * Ligase
 * Qiagen kits

Shared with other labs or borrowed to begin with

 * -80C Freezer (Chisholm)
 * gel reader box and computer, UV gel viewer? (Polz)
 * MilliQ water (Polz/Chisholm/Delong)
 * Other spectrophotometer (or portable A600 spec ~$700)

General

 * VWR stockroom at MIT : most equipment, chemicals, convenient but not necessarily cheapest, can pick up some items in 56-070 or deliver next-day, carry Owl gel equipment
 * ordered catalog Sean 16:27, 30 June 2006 (EDT)


 * USA Scientific Now in Ecat:Sciquest (like Eppendorf just cheaper) : plastic racks, ToughSpot labels, plasticware
 * registered Sean


 * ISC BioExpress : pipet tips, gloves, plasticware
 * registered and account approved Sean 17:02, 30 June 2006 (EDT)


 * Westnet : local source of Pyrex glassware, good service and cheapest


 * Fisher Scientific
 * ordered several pieces of literature Sean 15:59, 6 July 2006 (EDT)
 * registered with website but don't have account yet (phone or fax) Sean 12:34, 14 July 2006 (EDT)

Equipment

 * Eppendorf (centrifuges--tabletop and micro, pipettes, shaking dry baths, PCR machines), Meagan


 * Torrey Pines (incubators and dry baths)
 * rec'd catalog Sean 17:27, 12 July 2006 (EDT)


 * PCR machines: ABI for RT, Brinkmann Eppendorf rep = Meagan Hennessey (800-645-3050 x2681 or hennessey.m at eppendorf), Bio-Rad (bought MJ) rep Janusz_Wicher@bio-rad also for protein gel equipment, power supplies

Chemicals/Reagents

 * Sigma : chemicals as alternative to VWR, Pyrex supplier too
 * registered as new lab Sean 16:27, 30 June 2006 (EDT), received contract number for up to 70% off list, not posted for privacy
 * ordered more catalogs for equipment, etc. to add to Sigma and Life Sciences ordered earlier Sean 15:59, 6 July 2006 (EDT)
 * main catalog rec'd Sean 17:27, 12 July 2006 (EDT)


 * NEB/Fermentas : restriction enzymes, they have a set discount for MIT, same-day shipping if order by 10 am, $100 on-line orders get free shipping
 * catalog requested Sean 16:27, 30 June 2006 (EDT)


 * Qiagen kits : get through VWR stockroom or now through Ecat:Sciquest
 * ordered catalog Sean 15:59, 6 July 2006 (EDT)


 * Invitrogen: primers, Molecular Probes, free copy of Vector NTI, agarose, dNTPs, RT-PCR kits contact steve.smith@invitrogen for quote, should be $0.23/bp for 25 nmol scale, $0.37/bp for 50 nmol scale


 * GE Health Care division (formerly Amersham): radioactivity, dNTPs, templiphi


 * Stratagene: competent cells, kit for single base mutagenesis, Promega
 * requested catalog Sean 16:49, 30 June 2006 (EDT)


 * Ambion : RNA company
 * requested literature Sean 17:06, 6 July 2006 (EDT)


 * Macherey-Nagel DNA/RNA Purification kits
 * contacted US Sales rep, there is also a New England technical rep, they're sending catalog/prices Sean 15:59, 6 July 2006 (EDT)


 * Roche = protein


 * Pierce = protein

Misc

 * C & H for carts and bins
 * catalog requested Sean 16:27, 30 June 2006 (EDT)


 * Cole-Parmer: industrial equipment and supplies, fittings, tubing
 * requested catalog and registered (just username and pw) Sean 16:49, 30 June 2006 (EDT)


 * Upchurch: chromatography and fluid xfer components


 * ML Unique/hard-to-find useful lab products, have catalog

Links to many companies at Hopkins site

Used lab equipment sources

 * [labx] Marketplace, can place wanted ads
 * [ebay]
 * [Sci-Bay] Surplus/new

Repairs
Sean

shaking thermal block for microtubes

 * PHMT B0608 B0032
 * called 3/28/08 Grant Scientific 8009923028
 * 4/1/08 9084313028/31? Joe Costello will send replacement belt

Sean

autoclave

 * Ranger, Frank/Tom?
 * no cord, no exhaust pipe, rewired to single phase, have to go back to three-phase
 * Cambridge Scientific customer service problems