User:Anugraha Raman/Notebook/iGEM 2010/2010/06/23

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Wednesday June 23, 2010
amiRNA
 * Design amiRNAs using | online tool
 * Scheme for PCR
 * Go to the [| amiRNA section] for our primers for this multi step PCR or see chart below:

Transformations
 * Transformed ligations (V0210+LTP1) (V0210+Ger 3 sense) (V0210+Ger 3 antisense)w/turbo cells
 * Grew up colonies w/ RS300 for minipreps


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