User:Tara K. Luckau/Notebook/Team ConGen/2011/06/23

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PCR

 * similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers
 * [[Image:20110623 PCR.jpg|800 px]]

Gel

 * see 13 June 2011 for banding pattern expectations
 * [[Image:20110623 Gel.jpg|800 px]]
 * consistent with 13 June 2011 results (missing Scun22 band, but all others seem to be there)
 * notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient

SCOC Multiplex 1

 * SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux!

PCR

 * [[Image:20110623 PCR2.jpg|800 px]]
 * some of the samples used for the diversity panel are getting low ... should switch out soon
 * SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06

Gel

 * used left-over gel from 13 June 2011


 * see 13 June 2011 for banding pattern expectations
 * [[Image:20110623 Gel2.jpg|600 px]]
 * still a whole lotta nada :(
 * strong primer band, again
 * a bit of amplification of Scun9/10/11, but it's crap

Next Steps

 * try full gradient on multiplex, ugh


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