User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/09

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04/09/10

 * &#x2713; Minipreps: KAH142 rebuilt (4)
 * &#x2713; Assemblies: KAH146, 147 (H3me3 reporters) and KAH148-153 (Pc-ATF's w/o VP64, controls)

Minipreps > Check with E/P digests

Assemblies
 * 1) KAH146: (1) KAH96/(E/S/ApaL1)/2825 + (3) KAH145/(E/X)/~6755
 * 2) KAH147: (2) KAH142/(E/S)/4571 + "
 * 3) KAH148: KAH01/(E/S dp)/186 + (4) KAH116/(E/X)/~3975
 * 4) KAH149: KAH02/(E/S dp)/186 + "
 * 5) KAH150: KAH03/(E/S dp)/186 + "
 * 6) KAH151: KAH04/(E/S dp)/186 + "
 * 7) KAH152: KAH06/(E/S)/1170 + "
 * 8) KAH153: KAH07/(E/S)/1173 + "

> Digests (Fermentas FD) --> Triple digest KAH96 w/ E/S/ApaLI

> Measure conc.'s

> Ligations

--> R.T./ ~10 min.; Add to 20 μL Z-DH5α


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