User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/06/08

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Maize Endophyte Biofertilizers
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

The Daily Grind

 * To do: Replica plates (duplicates of 4 + 5 combined on all treatments) are 48 hours old now, and generally look like they've grown enough. For 24 hours more, they have been placed at 4 degrees to allow them to "age". Tommorow afternoon, I will take them out, pour siderophore solution on there, E. coli, bacilus, yeast, or perchloric acid onto RNA.
 * To do: Gel extracting restricted plasmids and doing overnight ligation (re-trying to fuse PSBA promoter to diff genes in pDRIVE)
 * To do: sequence all the positives from yesterday. There should be enough for a full 96 well plate.
 * To do: Inoculate fresh R2A broth with 1A1 and 2A10 for pouring on plants tommorow

Today, Sarah is scoring soil endophytes innoculated onto potatoes. I think its about half the population; these were not checked for OD, but they should give a general indication of whether these bacteria are pathogens or not.


 * }