IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week14/Chemical and Light

=Mystery Colony PCR 10/08= Colony PCR of putative fungal colonies using primers BBpfx and mtrB500.



Lane 1: 1 kb ladder

Lanes 2-6: P136 plate (500 bp)

Lane 7: P140 plate (1.9 kb)

Lanes 8-12: P122>131 plate (1.3 kb)

=Colony PCR 10/11= Unadulterated gel





Lanes 1-2: P148

Lane 3-4: P147 (picked 3)

Lanes 5-12: 147 (picked 6)



Lanes 13-14: P147

Lanes 15-24: P146 (picked 17, 23)



Lanes 25-26: P146

Lanes 27-36: P144 (picked 30, 35)



Lanes 37-39: P144

Lanes 40-48: P145 (picked 41, 46)

=Ligations 10/10=