IGEM:Harvard/2007/Protocols/Transformation Protocol


 * 1) Thaw competent cells on ice.
 * 2) Chill approximately 5 ng (2 μl) of the ligation mixture in a 1.5 ml microcentrifuge tube.
 * 3) Add 50 μl of competent cells to the DNA and mix gently by pipetting up and down.
 * 4) Incubate on ice for 30 minutes.
 * 5) Heat shock for 2 minutes at 37°C, chill on ice for 5 minutes.
 * 6) Add 950 μl of room temperature media and incubate at 37°C for 1 hour.
 * 7) Spread 100 μl onto the appropriate solid medium.
 * 8) Incubate overnight at 37°C.