User:Karmella Haynes

Contact Info


Dr. Karmella A. Haynes, Ph.D. Harvard Medical School Department of Systems Biology 200 Longwood Ave. WAB 536 Boston, MA 02115

phone: 617-432-6406 fax: 617-432-5012

karmella_haynes at hms dot harvard dot edu karmella.haynes at asu dot edu

Positions

 * 2011 (pending), Assistant Professor, School of Biological and Health Systems Engineering, Arizona State University

Education

 * 2008 - 2011, NIH NRSA Postdoctoral research fellow in Synthetic Biology, Harvard Medical School
 * 2006-2008, HHMI Postdoctoral research/ teaching fellow in Synthetic Biology, Davidson College
 * 2006, Ph.D. in Molecular Genetics, Washington University in St. Louis
 * 1999, B.S. in Biology, Florida A&M University

Links
Karmella's Links Haynes Lab at Arizona State University (coming soon) Openwetware Lab Notebook Genome Consortium For Active Teaching

2010 Harvard iGEM Team Team Wiki Harvard iGEM Blog

Research interests
Synthetic Biology The field of synthetic biology aims to engineer tiny machines, fashioned from characterized DNA and protein components, that perform useful functions, like synthesizing useful metabolites, attacking tumors, and detecting compounds in the environment. Currently as a postdoc in the lab of Pam Silver at Harvard Medical School, I am exploring the use of eukaryotic proteins as modular parts that can be used to build rationally designed devices in living cells.

Building Devices to Sense Developmental Cues So far, scientists have shown that molecular biology can be reconstructed to perform potentially useful tasks such as toggle switching, oscillation, pulsing, signal inversion, and multi-input processing. I am interested in developing simple modules that can feed useful biological information into these systems so that a device becomes activated or deactivated in response to developmental changes or disease. Two cellular cues that I am focusing on currently are miRNA expression and chromatin modification. Synthetic devices that can read cellular cues and generate therapeutic outputs (RNA, enzymes, etc.) will be a powerful tool for medicine.

Chromatin, An Untapped Resource for Parts Nature provides an abundant source of functional proteins for designing new systems. To date, chromatin proteins remain an untapped resource. Chromatin proteins called "effectors" have the remarkable ability to discriminate and bind to specific post-translational modifications of proteins called histones. Can a synthetic protein device be engineered to read histone modifications? Can we use this type of device as a new tool to monitor changes in histone modifications in single living cells? Accomplishing these goals will allow scientists to probe histone modification at unprecedented resolution, thus furthering our understanding of the dynamics of histone modifications associated with cancer and normal cell development.

Publicity
Calculating Bacteria: Real Computer Bugs? National Public Radio, Science Friday with Ira Flatow A group of scientists reports in the Journal of Biological Engineering that they have created specially modified E. coli bacteria capable of performing one specific type of calculation — a puzzle known as the "pancake flipping problem." Karmella Haynes, one of the researchers, discusses the prospects for biologically based computing, and ways in which calculating bacteria might be useful.

Synthetic Biologist Karmella Haynes WBUR Teacher's Domain This video produced for Teachers' Domain profiles Karmella Haynes, a post-doctoral researcher working in the emerging field of synthetic biology. Karmella explains how she uses biotechnology to build living machines, or devices, from genes.

Publications
Research
 * 1) Synthetic reversal of epigenetic silencing Haynes KA, Silver PA. (2011)  J Chem Biol. E-pub ahead of print. PMID: 21669865 Cover article
 * 2) Engineering bacteria to solve the burnt pancake problem Haynes KA, Broderick ML, Brown AD, Butner TL, Dickson JO, Harden WL, Heard LH, Jessen EL, Malloy KJ, Ogden BJ, Rosemond S, Simpson S, Zwack E, Campbell AM, Eckdahl TT, Heyer LJ, Poet JL. (2008)  J Biol Eng. PMID: 18492232 JBE Publication of the Year, 2008
 * 3) An investigation of heterochromatin domains on the fourth chromosome of Drosophila melanogaster Riddle N, Leung W, Haynes KA, Granok H, Wuller J, Elgin SC. (2008) Genetics. PMID: 18245350
 * 4) A distinct type of heterochromatin within Drosophila melanogaster chromosome four Haynes KA, Gracheva E, Elgin SC. (2006) Genetics. PMID: 17194780
 * 5) Element 1360 and RNAi components contribute to HP1-dependent silencing of a pericentric reporter Haynes KA, Caudy AA, Collins L, Elgin SC. (2006) Current Biol. PMID: 17113386
 * 6) cis-Acting determinants of heterochromatin formation on Drosophila melanogaster chromosome four Sun FL, Haynes K, Simpson CL, Lee SD, Collins L, Wuller J, Eissenberg JC, Elgin SC. (2004) Mol Cell Biol. PMID: 15340080

Reviews
 * 1) Eukaryotic systems broaden the scope of synthetic biology Haynes KA, Silver PA. (2009)  J Cell Biol. PMID: 19948487
 * 2) Analyzing heterochromatin formation using chromosome 4 of Drosophila melanogaster Haynes KA, Leibovitch BA, Rangwala SH, Craig C, Elgin SC. (2004) CSHSQB. PMID: 16117658

Science Art Gallery
In addition to science research, I paint and draw. Below are pieces that have a scientific theme. You can view my other work at http://www.karmellahaynes.com