User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/30

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Halometabolites from uncultured bacteria
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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TAR cloning of natural product gene clusters

 * ZB118 subcloning project is underway. The capture vector was mapped with DraI which releases the two targeting sequences for comparison along with a standard fragment map.  All clones look fine.  JHK and TEG pathway capture vectors also report patterns that are fine (see gel images)
 * YAC's isolated from single cells on the cycloheximide selection plates (1:4000 dilution overlay) are in very low concentration. Transformation into EC100's (homebrew) did not yield (#)$.  Chemically competent cells also did not yield $%.  (size restriction for top 10's potentially limiting).  e


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