Kubke Lab:/Notebook/Cranial nerve development/2011/02/03

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Cranial Nerve Development
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=General Entries= =Personal Entries=
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Malisha

 * 10.00 AM: Opened a google doc to plan studentship report
 * 10.30 AM: Working on neuroanatomy document. Goal is to complete the necessary components by tomorrow--Malisha Hettiarachchi 17:04, 2 February 2011 (EST).

Reuben

 * 10:00am Photographed embryo MH007
 * Severed embryo rostral to wing bud and re-photgraphed.
 * Placed embryo in large metallic mould half-filled with OCT.
 * Incubated mould in the cryostat at -19°C.
 * Loaded the mould block onto a metallic chuck and secured it in the metallic chuck holder.


 * Built up OCT slowly to support the large block.
 * Using a razor blade, by hand, I trimmed the block into a cone shape and removed enough OCT so that the block could fit between the chuck holder and the knife.
 * 12:00pm Began incubating the final assembly at -19°C in the cryostat.
 * Went to lunch.
 * Entered in todays journal entry and added page for populated the MH007 page with the appropriate templates.--Reuben Cutfield 21:02, 2 February 2011 (EST)
 * 1:00pm Began cutting embryo MH007.--Reuben Cutfield 18:53, 2 February 2011 (EST)




 * 4:10pm Finished cutting MH0007. Will stain the slides tomorrow. --Reuben Cutfield 22:14, 2 February 2011 (EST)

Fabiana

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