Template:SBB09 19329

Jong H. Shin

upaG Autotransporter long version
Source: CFT073 genomic DNA First of all, read PMID: 18424525. Also download this template for your design [[Media:JCA_upaG.seq | here]]. You are going to make the part that includes the "Him region" (blue) and the "L1 L2 beta" region (red).

Due to the large size, you won't sequence every base of your part. You should do a reverse read with G00101 to confirm that the "L1 L2 beta" region is correct in your part, and you should also do a forward ca998 read over the N-terminus. Ignore the middle.

Alkaline Phosphatase
Source: pBca9145-Bca1126  {} The oligos to construct this part have already been designed. You just need to complete the construction file below. As background, you should read PMID: 7929016. The map of the template sequence is [[Media:pBca9145-Bca1126.seq | here]]. PCR mea036/mea037 on pBca9145-Bca1126		(1219 bp, EcoRI/BamHI) Sub into ????? (EcoRI/BamHI, ???, ???) Product is ????

mea036  Forward Biobricking of {}	cgatagaattcatgAGATCTGGAGATTCTG mea037  Reverse Biobricking of {}	cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG