IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/02

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8/2/10
10:00 AM

got a good amount of colonies, a few white colonies on all plates. will pick for 5 mL overnights this afternoon.

5 mL overnights of ArsR-B0015-pSB1A2 spilled, 1 tube also appeared green...

made glycerol stock of ArsR-B0015-pSB1A2 colonies 1 and 2, meagan did miniprep

ArsR-B0015-pSB1A2 colony 1: ISB214

ArsR-B0015-pSB1A2 colony 2: ISB215

PCR Insert Verification

Run by Meagan

Program:

Primers:

20 micromolar VR VF2

Samples:

LamBF2R

LamBF2R Green

ArsR-B0015-pSB1A2

negative control (no template)

10:25 PM

PCR

Per reaction: 19 μL mastermix, 1 μL template

Program:

Primers:

ArsBF2

ArsBR2

Samples:

ArsB48°C

ArsB51°C

ArsB54°C

ArsB57°C

ArsB60°C

ArsB63°C

ArsB66°C

Negative control (no template)

1:05 PM

Gel

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