IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/08/19

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cPCR preparaion: summary

 * innoculated and made spot plate of constructs from Aug 19
 * The constructs were:
 * pSB1C3 + LoxR
 * pSB1A3 + LoxR
 * pSB1C3 + Cre-LVA
 * pSB1A3 + Cre-LVA

= The innoculation = performed in triplicate using standard UBC iGEM protocol
 * picked 3 colonies from each plate, each colony into 5mL LB with appropriate antibiotic (Ampicillin for pSB1A3 constructs and chloramphenicol for psB1C3 constructs)
 * to test that the antibiotics are effective, also had negative controls: innoculated a pSB1A3 colony into LB-Chlor and a pSB1C3 colony into LB-Amp


 * overnight innoculation


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