User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/13

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08/13/10

 * &#x2713; Order oligos: sequencing primers for pActin5C (based on sequence from Kent Nybakken)

Order oligos > Received full sequence of "pActin5C" plasmid from Kent Nybakken > Order oligos to sequence across multiple cloning sites
 * 1) pA5Cseq f3; 5'-CACTACCGTTTGAGTTCTTGTG
 * 2) pA5Cseq f4; 5'-CTTGTGCTGTGTGGATACTCC
 * 3) pA5Cseq r3; 5'-GCTGGAAGGTGGACAGCGAAGC
 * 4) pA5Cseq r4; 5'-GCGAAGCCAGGATGGAGCCAC


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