Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF using HotStar

Rxn Conditions
Annealing Temperature: 50oC (5 degrees below 2736 annealing temp)

Extension Time: 1 minute (1 min/kb, 804 bases)

Cycle

7.23.08
I ran two PCRs to amplify the HmgR ORF: one with Buffer Q and the other without. I made a master mix, then aliquotted 40 &mu;l to each of two tubes. I then added 10 &mu;l Buffer Q to one and the same volume of water to the other. Used the reaction conditions as written. Gel results here.

8.2.08
I ran one PCR to amplify the HmgR ORF with buffer Q. I just made the reaction as in the protocol and ran with the conditions written above.