Escherichia coli/Knockouts

The Keio collection is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the Functional Genomic Analysis of E. coli in Japan project Baba-MSB-2006.

These strains are currently available from a number of sources:

The National Institute of Genetics in Japan, or the E. coli Genetic Stock Center (CGSC).

The entire collection as a set can also be obtained from Open Biosystems

Alternatively, you can obtain the relevant plasmids and strains for doing the knockouts yourself from the CGSC (the "Wanner gene disruption kit") Datsenko-PNAS-2000.

Source

 * Email correspondence between John Cumbers and Wilson Wong.