Klapperich Lab:Notebook/Lab Meeting Notes/2009/06/02

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2 June 2009 Lab Meeting
† Announcements Suggested groups... please edit. Teams 1. PCR= QQ, MCK, MM 2. Bacteria = JZ, JD 3. Flu = MM, JZ, HM, JC, BC 3. Lysis? = AG, HM? † Flu R01 † Coulter Flu Fraunhofer Project † SEPSIS Project †RNA project † COBRA paper 1: Evap with Sol Gel substrate. paper 2: Covap with PMA, Rhodamine, PS beads?...virus...? † Virus concentration † Valve Array † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant
 * SIGN UP FOR NEW LAB DUTIES WITH SONALI.
 * Short small team meetings. 45 min each week?
 * MicroTAS Abstracts accepted. (4) Papers due June 30th.
 * RNA extraction troubleshooting.
 * Absolute Quantification assays: TH this week.
 * FTIR
 * Fraun folks coming to get trained next week. RNA isolation.
 * IBC for Fraun flu. approved?
 * Revision submitted 5/20.
 * Jeff visiting TH
 * Test with sol-gel substrate today(2nd June)
 * delayed due to difficulty adjusting membrane thickness
 * Evap system (JD and JZ)
 * Let's get this to a point where we can publish and move on
 * Teddy will give training presentation(guide).
 * Paper revised.
 * Bonding ongoing with MIT folks
 * Schedule a meeting next week of all collaborators to discuss submission details.
 * found a method of double stacking SU-8 using mask-aligner for making Herringbone grooves.
 * investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
 * asked Brett to take a photo of the device for the paper.
 * MC and Jason will get SEM images of PDMS mold with thin gold coating of nano-size on its surface.
 * draft paper2 will be started soon.
 * IRB still in revision.

† PCR
 * PCR 2 paper draft.
 * developed PEG-coating protocol, need do on-chip experiment. Do you need to use PEG-NH2? hoe about just PEG terminate PEG?

† RCA/HDA
 * Getting solution back out is still an issue.
 * Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime)
 * Evaporation problems near edges. Maybe design change?
 * Teflon issue with the enzyme? Check into it.

† Silica Optimization (Lambda):
 * Present Bioan. Data to Jeff.
 * Get lower Bioan. Kit.


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