User:Meng Xiao He/Notebook/fall08/2008/11/14

{| width="800"
 * style="background-color: #F2F2F2" align="left"|  |Main project page
 * style="background-color: #F2F2F2" align="left"|  |Main project page


 * colspan="2"|
 * colspan="2"|

PCR results
Top 2 lanes in ladders are 2kb and 800bp


 * 1st 8: cco in pER21 candidate
 * 2nd 8: cbb3 in pER21 candidate
 * 3rd 8: Duo in TOPO C
 * last 8: TOPO B, different colonies
 * TOPO B3, B6, B7, and B10 seem correct
 * Not quit sure what the other bands are, but the TOPO C stuff looks that size??


 * none of the conditions look particularly different, but this is a low res. gel
 * temperature 7 seems like a good place to start
 * alarmingly not clean
 * TOPO C looks like it might only have 1/2 of what it's supposed to?


 * }