User:Tk/Notebook/MF/2008/10/31

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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 * style="background-color: #F2F2F2" align="center"|  |Main Page


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Part cloning

 * J70023, J70023-F2 into pSB1AK3
 * J70024, J70025 into pSB1AC3
 * J70026 into pSB1AK3
 * J70007 into pSB1AK3

Backbone construction

 * PCR, column cleanup (100 ul volume, elute to 50 ul, digest at 100 ul)
 * digest, heat kill, add 150 ul beads, add 500 mM salt, 1 mM EDTA, volume at 276 ul)
 * bind, remove beads
 * dilute with 5x PB (1.4 ml), column bind total of 1.7 ml, 3x column loading
 * elute


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