Laub:PYE

Rich Media for Caulobacter crescentus

Ingredients

 * 2.0g bactopeptone
 * 1.0g yeast extract
 * 0.3g MgSO4
 * From Sigma
 * 10mL 50 mM CaCl2
 * From Emanuele: not necessary for Caulobacter growth at MIT (perhaps enough calcium already in yeast extract or kitchen flasks).
 * Now we use 1mL of 0.5M CaCl2 from the MIT Kitchen --Sri Kosuri (talk) 16:49, 19 April 2007 (EDT)
 * 1L H2O (milliQ or distilled)

Instructions

 * Add ingredients and autoclave (250&deg;F, 22psi, 30-60min depending on volume).
 * For plates, usually add 1.5% (w/v) BactoAgar as well.