IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/03/12

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Objective: Ask for information about LovTap
I have been in touch with Dr. Devin Strickland the designer of LovTAP, in order to get advices to work with LovTap.

He recommended us the following:

You should take a look at Figure 2.4.2 of my dissertation [1] and try to figure out why that experiment didn't work. You may understand it already, but my interpretation is that LovTAP was being expressed at too high a level. I'd suggest getting the expression level down as much as possible. Also, whatever your reporter is (LacZ, GFP, etc.), I'd recommend putting a degradation tag on it, as in the classic Elowitz and Leibler "Repressilator" system.

See below the figure 2.4.2 information that Devin cited.