Kubke Lab:Research/CND/Records/RC005

=Embryo details=

Species: Gallus gallus domesticus Embryo Name: RC005 Embryo stage: ST19 Fabiana staged this embryo on the 25/1 and confirmed it was a ST19. --Malisha Hettiarachchi 15:22, 27 January 2011 (EST)

Staging description: Originally staged by Reuben as a ST20, but Fabiana restaged on 25/1as a ST19 and Fabiana according to the Hamburger and Hamilton (1951) staging system. The description below corresponds to Reuben's staging.
 * Wing and leg buds barely formed.
 * The leg is slightly longer than the wing.
 * Allantois has vesicularized and is roughly the same size as the midbrain (indicative of ST20).

(see Notebook entry 10th January 2011) Fixation: Cryoprotection: Was stored in PFA, staged in .9% saline and then replenished with PFA. Material label and storage:Stored in vial filled with PFA labeled RC005 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.

=Experiment details=

Objective:To use as a practice embryo for staging. Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage. Comments:

=Results=

Embryo is approximately ST20.

=Images= =Summary=

This embryo may be used in further experiments as approximately a ST20 however confirmation is needed.