Template:SBB10 ConstructionFiles JoannaC sbb17

Construction of Zinc Finger Domain (zf+) (sbb17)

Pool jc01 through jc16, assemble by PCA PCR jc01/jc08 on PCA reaction	(424 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144	(EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb17      {}

jc01    PCA Assembly of sbb17 and sbb18     AGTTCGAATTCATGAGATCTGCACCTAAGAAGAAACGCAAAGTGGGTATCC jc02    PCA Assembly of sbb17 and sbb18     GCCATTGCTGCTGGTACTCCATGGATACCCACTTTGCGTTTCTTCTT jc03    PCA Assembly of sbb17 and sbb18     TGGAGTACCAGCAGCAATGGCCGAACGTCCATTCCAGTGTCGTATTTGCA jc04    PCA Assembly of sbb17 and sbb18     TATCAGAGCGGGAGAAATTTCGCATGCAAATACGACACTGGAATGGAC jc05    PCA Assembly of sbb17 and sbb18     GCGAAAAGCCGTTCGCTTGCGACATTTGTGGACGTAAGTTTGCC jc06    PCA Assembly of sbb17 and sbb18     TCCGCATGCAGATCCGGCATTGGAAAGGTTTTTGACTACCAGTGT jc07    PCA Assembly of sbb17 and sbb18     TTCGCACTCACACAGGAGAAAAGCCTTTCGCGTGTGATATCTGCGGAC jc08    PCA Assembly of sbb17 and sbb18     GTTCAGGATCCACGGAGGTGAATTTTTGTGT jc09    PCA Assembly of sbb17               CGAAATTTCTCCCGCTCTGATACTCTGTCTGAACACATCCGAACGCAC jc10    PCA Assembly of sbb17               GCAAGCGAACGGCTTTTCGCCAGTGTGCGTTCGGATGTGTTCAGAC jc11    PCA Assembly of sbb17               GATCTTCGTATGAGTAGTCCGTGTACTTCGGGCGGCAAACTTACGTCCACAAATG jc12    PCA Assembly of sbb17               TACACGGACTACTCATACGAAGATCCACACTGGTAGTCAAAAACCTTTCCA jc13    PCA Assembly of sbb17               TGCCGGATCTGCATGCGGAATTTTAGCCGTAGTGACTCGCTCAGC jc14    PCA Assembly of sbb17               GCTTTTCTCCTGTGTGAGTGCGAATATGCTTGCTGAGCGAGTCACTACGG jc15    PCA Assembly of sbb17               CCTTGAGATTTGATCGCTGTGCGAACTTTCGTCCGCAGATATCACACGCGA jc16    PCA Assembly of sbb17               TCGCACAGCGATCAAATCTCAAGGTGCACACAAAAATTCACCTCCGTGGATC



JCA Notes

 * Correct