User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/26

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03/26/10

 * &#x2713; Minipreps: KAH144 (4)
 * &#x2713; Assembly: KAH145

Minipreps > Check with E/P digests

Assemblies
 * 1) KAH145: (1) KAH144/(S/P)/~5599 + KAH51/(X/P)/1160 &#x2713;

> Digests (Fermentas FD)

> Measure conc.'s

--> KAH51 (X/P) = 42.9

> Ligations

--> R.T./ ~10 min.; Add 50 μL Z-DH5α


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