Shreffler:Notebook/retinoic acid trans signaling/2009/07/30

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Retinoic Acid Trans Signaling
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Neisha A. Rivers
Measuring Luciferase


 * Transfer cells to 1.5 ml eppendorfs
 * Spin cells down for 500g for 10 min
 * Aspirate supernatants, then added 125 ul of PLB buffer, vortex
 * Allow to shake at room temperature for 30 min
 * Spin cells at 1400g for 2 min, leave supernatants
 * Add 25 ul of substrate (LAR) and 5 ul of lysate (supernatants)

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Results: Luciferase was not detected in any of the samples

Next Steps: Beta-gal assay, and a different transfection method - Lipofectamine


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