Sean Clarke/109 notes

20.109:Module_1

M13 genome
6407 bp single-stranded circular DNA

filamentous, male-specific? coliphage related to fd and f1


 * p3,p8,p9 = virion structural proteins, p3 and p8 have N-term signal peptides that are hydrophobic

11 coding genes: 5 on surface, 6 for phage maturation in host

oligos
NO150/151

g8p myc' Top / Bottom

CTC GAA CAG AAG CTT ATC TCT GAA GAA GAC CTT GCA

ACG TGA GCT TGT CTT CGA ATA GAG ACT TCT TCT GGA

NO154/155

g3p myc' Top / Bottom

GAT CTA GAA CAG AAG CTT ATC TCT GAA GAA CTA

ATC TTG TCT TCG AAT AGA GAC TTC TTC TGG ATC TAG