User:Tara K. Luckau/Notebook/Team ConGen/2011/03/18

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Scun2 FAM Diversity Panel PCR

 * chose Buffer G, 58°C as 'best' condition for Scun2 primers (with FAM)
 * now run diversity panel and submit to fragment analysis

Diversity Panel
Sceloporus occidentalis from all five sites (plus two redundancies)
 * SCOC RAJ X01 - Rancho Jamul
 * SCOC HOL X01 - Hollenbeck
 * SCOC SYR2 X01 - Santa Ysabel
 * SCOC CPN X01 - Camp Pendleton
 * SCOC TP1 X01 - Torrey Pines
 * SCOC TP3 X01 - Torrey Pines
 * SCOC LOM X01 - Point Loma

PCR

 * First PCR to send to fragment analysis!
 * use labelled forward primer (Scun2 F FAM)
 * increase reaction volume (10µL to 15µL) to have enough to ship to Tucson after confirmation gel
 * gel results from gradient PCR was a bit weird (only Buffer G yielded any product), so use three buffers: F, G, H
 * this also makes shipping to Tucson worth the money


 * [[Image:20110318 PCR.jpg|800 px]]
 * Used thermal cycler in room 215 (because used strips with caps, not plate)