User:Anugraha Raman/Notebook/iGEM 2010/2010/06/21

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Entry title

 * Lab Presentation
 * Primer Design
 * (Amplify 300 bp exon following our intron in Fra a 1)
 * Took first/last 20 sequences of exon
 * (Amplify out intron for use (but this contains biobrick cloning sites so we probably won't do this)
 * Took last 5 sequences in exon, first 15 sequences in intron and last 15 sequences in intron and first 5 of following exon)
 * GFP Sequence Alignment


 * PCR (Bet v1, Bet v 1.2, Ger 3, LTP 1)
 * Set annealing temp for 67.5
 * Used ~ 100 ng of genomic aribidopsis DNA/ 50 uL reaction
 * Lanes (1:Ladder; 2:LTP Sense; 3: LTP Antisense; 4:Ladder; 5:Bev v1 Sense; 6:Bev v1 Antisense; 7:Ladder; 8:Bev v1.2 sense; 9:Bev v1.2 antisense; 10: Ladder; 11:Ger 3 Sense; 12:Ger 3 Antisense)