Registry/Measurement kit/Notebook/2007-7-8

M/N Digest of I2055

 * Hopefully it has the promoter
 * Ligated into 1AK3 and transformed.

Colony PCRs

 * Running a gel
 * loading order:T9002 in 3K3 (4-1), I2055 in 1AK3 (1-4), E0240 in 3K3(4-1), E0240 in 1AK3(1,2).
 * Seems to be a problem with 3K3 (perhaps not digested fully?)
 * Making cultures to sequence:
 * T9002-3K3(bad): #3
 * I2055-1AK3: #1
 * E0240-3K#(bad): #3
 * E0240-1AK3: #1
 * Since 3K3 may be causing the problems, making o/n of it to mini-prep tomorrow and digest.

I2056

 * Prepared sequencing reaction.