Julius B. Lucks/M9 Supplemented Minimal Media

This protocol is a derivative of Endy:M9_media/supplemented. Endy protocol items in black, our protocol items indented and in red.

Protocol
For 1L of media
 * 500ml 2xM9 salts
 * used 11.28g of M9 Minimal Salts in 500ml of MiliQ-filtered H2O
 * 30ml 10 mg/ml thiamine hydrochloride
 * used 300 mg thiamine hydrochroride
 * 10ml 40% glycerol
 * used 8ml 50% glycerol
 * 20ml 10% casamino acids
 * wasn't sure if this was by weight or not. Assumed 10% by weight, so used 2g casamino acids.
 * 20ml 0.1M MgSO4
 * used 0.493 g (FW 246.49 g/mol)
 * 200&mu;l 0.5M CaCl2
 * used the same (made 1ml 0.5 M CaCl2 with 74 mg (FW 147.01 g/mol))
 * 419.8ml sterile deionized H2O
 * we made the above solution and filled to 1L with MiliQ-filtered H2O

We combined the above ingredients, and filtered through a milipore steritop filter into a clean auto-claved 1L bottle. Stored at 4&deg;C