Carrico:Recipes



2XYT Media (to make 1L)

 * 1) Combine: 900mL distilled water, 16g Bacto Tryptone, 10g Bacto Yeast Extract, 5g NaCl
 * 2) pH to 7.0
 * 3) Add water up to 1L
 * 4) Autoclave

50x TAE Buffer (to make 1L)

 * 242g 2M Tris base
 * 57.1mL 1M glacial acetic acid
 * 200mL 100mM EDTA
 * Add water up to 1L

Note: To dilute 50x to 1x, add 20mL of 50x to 980mL water

10x Laemmli Running Buffer (Tris glycine)

 * 30.3g Tris base
 * 144g glycine
 * 10g SDS
 * Add water to 1L

Coomassie Brilliant Blue (CBB)

 * 1) Dissolve 0.25g CBB in 90mL methanol:water (1:1 v/v) and 10mL glacial acetic acid
 * 2) Filter through Whatman #1 filter paper to remove particulate matter
 * 3) Stain gel for at least 4 hours in 5 volumes of CBB

Protein Gel Destain Solution

 * 125mL methanol
 * 35mL glacial acetic acid
 * Add water to 500mL
 * Store at room temperature
 * Destain for up to 24 hours