Knight:Preparing stabs

For long term storage of bacterial strains. Useful for shipping strains.

Materials

 * Stab agar
 * 10 g nutrient broth
 * 5 g NaCl
 * 6 g agar
 * 10 mg cysteine&dot;Cl
 * 10 mg thymine
 * H2O to 1 L

Autoclave to sterilize.

Procedure

 * 1) Fill airtight, autoclavable vials with rubber or teflon caps 2/3 full of stab agar
 * 2) Inoculate with a single colony repeatedly poking the inoculating loop into the agar
 * 3) Incubate at 37&deg;C for 8-12 hrs until cloudy tracks of bacteria become visible
 * 4) Seal tightly and store in the dark at room temperature (15-22&deg;C)
 * 5) Revive by using inoculating loop to streak onto a new LB plate