IGEM:The Citadel/Notebook/Construction

Biobrick construction map:

1: B0015 (A) -> J04450(C) = B0015 (C, ex A) -- transformed and plated. Minipreped. 2: C0160 (A) -> J04450(C) = C0160 (C, ex A) -- transformed and plated. Minipreped. 3: F1610 (A) -> J04450 (C) = F1610 (C, ex A) -- transformed and plated. Minipreped.

4: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K) -- transformed and plated. 5: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K) -- transformed and plated. 6: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K) -- transformed and plated. 7: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K) -- transformed and plated. 8: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K) -- transformed and plated.

9: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A) 10: ~I50B15 (K) + I746360 (A) -> J04450(C) = K274370 (C) 11: ~I51B15 (K) + I746361 (A) -> J04450(C) = K274381 (C) 12: ~I52B15 (K) + I746365 (A) -> J04450(C) = K274395 (C)

13. F2620 (A) + K274382 (C) -> J04450(K) = ~F20K82 (K) 14.

Biobrick Inventory:

1. J04450 (C) - 08/18 - 355.9 ng/ul