IGEM:Peking University/2008/Notebook/Group 1/2008/07/18

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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PKU_080718_G1_1

 * Enzyme cutting-pADH-lac2(PCR product)


 * System for double ligation


 * System for single ligation


 * 'PCR- pGREG504-pADH-(lac1/lacS/tet)-50ul
 * Using program lac12MQT


 * Gel running and extraction-promoter(PCR product)


 * Enzyme cutting-AID


 * lanes: intact AID(control)|λmarker|Promoter(single)|promoter(double)|AID|AID


 * System for double ligation


 * System for single ligation


 * Single Ligation-pGREG504-pAID-lac2


 * Double Ligation-pGREG504-pAID-lac2-AID


 * Gel running-testing-pADH-lac1/S/tet
 * Gel running-testing-digestion-lac2/AID


 * }