IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/06/Shuke Wu

Plasmid mini prep 6 RBS; lacI; araC;

Double digest 6 RBS: Spe1 1uL, Pst1 1uL, plasmid 4uL, Buffer 2uL, water 12uL lacI, araC: Xba1 1uL, Pst1 1uL, plasmid 4uL, Buffer 2uL, water 12uL 37 ℃ 4 hour