Van Oudenaarden Lab:ZK993.1

Progress:

Probes designed: (03-01-2010 - Alexander van Oudenaarden)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

>ZK993.1 (ceh-45 spliced coding region)

actgaagaatcgcatcccccactacggtgtgacac

atggcatccttcccgtttttaatgacacctgaaactatgaacaaattggtgacgccgaattctgcaagtatctcctcgtc atcctcctctccgtccacatcaacctcattctcaattgacacattgctctcgaacccgttggccggaattccgcagcttc acaattttcaagatccatttggagcagtgggagccgcagcggcggcttctgccctgccatggcagtttcatccggctaat tatccgtttttctcaatgctctgcggaccactaatgcctccattcatggctccatatcaccatcaacactacgcctcccg acgaaaacgccggcatcgtaccattttcagcgaggagcagttgaacattttggagaccacattttcaacgacacactacc cggacgctacgacgagagaagagctcgcggttcagtgctcattgaaggaagaaagagttgaggtctggttcaaaaatcgt cgggcaaaagagcgcaagcagaagaaggacgacagtcggacttctaagcacagcggagacgattccgaatgtgacgagag cgacgaggacacccgaaaagtgaaacggataaaacgcgaaaattccagcggaaaagagacgagctcgcccgaatcaaaag cctcactgaagacatcgcacagcgagaatttggtgttggagccttgttag

catataatttgaatctctattccccacctagctggaaatccaggccacc ccctctttatattctagtcccccccatcccctctcattttgtcaagtcaataaa

Name of probe set: ceh-45

Probes designed on Mon, 01 Mar 10 13:49:58 -0700

37 probes designed for target of length 828 (target too short for 48 probes)

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

gggggatgcgattcttcagt, ceh-45_1, 1, 1, 55

atgccatgtgtcacaccgta, ceh-45_2, 2, 23, 50

caggtgtcattaaaaacggg, ceh-45_3, 3, 47, 45

cgtcaccaatttgttcatag, ceh-45_4, 4, 70, 40

aggagatacttgcagaattc, ceh-45_5, 5, 92, 40

gtggacggagaggaggatga, ceh-45_6, 6, 114, 60

gtcaattgagaatgaggttg, ceh-45_7, 7, 136, 40

ccaacgggttcgagagcaat, ceh-45_8, 8, 158, 55

attgtgaagctgcggaattc, ceh-45_9, 9, 181, 45

ctgctccaaatggatcttga, ceh-45_10, 10, 203, 45

agaagccgccgctgcggctc, ceh-45_11, 11, 226, 75

gatgaaactgccatggcagg, ceh-45_12, 12, 248, 55

gagaaaaacggataattagc, ceh-45_13, 13, 270, 35

cattagtggtccgcagagca, ceh-45_14, 14, 292, 55

gatatggagccatgaatgga, ceh-45_15, 15, 314, 45

tcgggaggcgtagtgttgat, ceh-45_16, 16, 337, 55

tggtacgatgccggcgtttt, ceh-45_17, 17, 359, 55

ttcaactgctcctcgctgaa, ceh-45_18, 18, 381, 50

cgttgaaaatgtggtctcca, ceh-45_19, 19, 406, 45

tcgtagcgtccgggtagtgt, ceh-45_20, 20, 428, 60

tgaaccgcgagctcttctct, ceh-45_21, 21, 450, 55

tctttcttccttcaatgagc, ceh-45_22, 22, 472, 40

gatttttgaaccagacctca, ceh-45_23, 23, 494, 40

tgcttgcgctcttttgcccg, ceh-45_24, 24, 516, 60

agtccgactgtcgtccttct, ceh-45_25, 25, 538, 55

aatcgtctccgctgtgctta, ceh-45_26, 26, 560, 50

tcgtcgctctcgtcacattc, ceh-45_27, 27, 582, 55

ccgtttcacttttcgggtgt, ceh-45_28, 28, 604, 50

cgctggaattttcgcgtttt, ceh-45_29, 29, 626, 45

tcgggcgagctcgtctcttt, ceh-45_30, 30, 648, 60

tgtcttcagtgaggcttttg, ceh-45_31, 31, 670, 45

acaccaaattctcgctgtgc, ceh-45_32, 32, 692, 50

attatatgctaacaaggctc, ceh-45_33, 33, 714, 35

taggtggggaatagagattc, ceh-45_34, 34, 736, 45

agagggggtggcctggattt, ceh-45_35, 35, 761, 60

gatgggggggactagaatat, ceh-45_36, 36, 783, 50

ttgacttgacaaaatgagag, ceh-45_37, 37, 805, 35