IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-28

Western blot images
Western blot images without the annoying rectangle:



Miniprep of J36335 (KaiA + KaiC), J36832 (KaiB)
We performed 8 minipreps each of J36335 and J36832, eluted in 30 µL EB.

Digest of J36335\S-P (KaiA + KaiC), J36832\X-P (KaiB)
We did 8 digests each of J36335\S-P and J36832\X-P.

Reaction
J36335\S-P
 * 7.6 µL DNA
 * 1 µL 10x BSA
 * 1 µL NEBuffer 2
 * 0.2 µL SpeI
 * 0.2 µL PstI

J36832\X-P
 * 7.6 µL DNA
 * 1 µL 10x BSA
 * 1 µL NEBuffer 3
 * 0.2 µL XbaI
 * 0.2 µL PstI

Master mixes
J36335\S-P Aliquot 10µL into each tube.
 * 76 µL DNA
 * 10 µL 10x BSA
 * 10 µL NEBuffer 2
 * 2 µL SpeI
 * 2 µL PstI

J36832\X-P Aliquot 10µL into each tube.
 * 76 µL DNA
 * 10 µL 10x BSA
 * 10 µL NEBuffer 3
 * 2 µL XbaI
 * 2 µL PstI

Presentation notes

 * More pictures in general-- picture of cyanobacteria
 * Slide with Western blot: label it with conclusion of experiment
 * Cut "Interaction experiments" slide; copy Kai model to results page, delete arrows to show predicted effect
 * Conclusion slide, or at least say some closing words before going on to acknowledgements