User:Karmella Haynes/Notebook/Polycomb project/2010/07/15

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07/15/10

 * &#x2713; Senescence assay: flow cytometry on 8-day -/+ dox samples

Senescence assay > Samples: 1,2. KAH126-1 -/+ dox 3,4. KAH126-3 -/+ dox 5,6. KAH132-8 -/+ dox 7,8. KAH154-2 -/+ dox 9,10. KAH128-8.3 -/+ dox 11,12. KAH129-4 -/+ dox

> Treat w/ 1.5 μg/mL C12-FDG/ 37°C/ 45 min. before harvesting for flow cytometry > Wash once with DPBS and resuspend in 0.5 mL DPBS > Spin through filter-cap


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