IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/29

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] Magnetic Bacteria
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Biobrick Handling...

 * I0500 promoter and pSB3K3 plasmid PCR-ed following previous protocol
 * 0.8% agarose gel with SyBr Green used
 * Lane 4 - Hyperladder I
 * Lane 5 - I0500
 * Lane 6 - I0500 cut with SpeI
 * Lane 8 - pSB3K3
 * Lane 9 - pSB3K3 cut with EcoRI and PstI

Result: Unknown band shown on lane 8 with a much smaller size (400bp) than expected. Faint band on lane 6 (1200bp) corresponding to I0500 gene size. Other lanes didn't work.


 * }