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Figure 1 Preferential apoptosis of cells with LTD4 and TNF-α activated pathways. The RNA construct is localized in the nucleus and its activity is controlled by alternative splicing. The Ex7 sequence contains a stop codon and if included in the post-processed RNA greatly diminishes the product of the downstream gene of interest (in this case HSV-TK). If Ex7 is repressed then the splicing modification removes that segment and the subsequent RNA product is highly transcribed. In this case HSV-TK, the gene of interest, increases the likelihood of apoptosis when GCV is also present. This way, in two distinct steps an AND gate is created where both GCV and the activated pathway are needed.