User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/03

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Part 1: Hybridization of Probe to DNA during Southern

 * Performed by MICB323 Instructors
 * Goal: To get DIG-labeled probe onto the membrane

Part 2: Detection of Probe using DIG Luminescent Detection System

 * Sunny did most of this part, while I did all of the 24-well plate and harvesting + Polyacrylamide Gel
 * After comparing Sunny's x-ray film to my own, we decided to use my film + gel image for the report.

Part 3: LPS +/- IFN-g Stimulation of Murine Macrophages

 * Macrophages were all prepared for us in DMEM media.
 * Sunny did "Student A" work - generating pooled samples for 24 hr stimulations.
 * I did "Student B" work - generating supernatants for analysis via ELISA.
 * I did not follow the protocol exactly. Rather than generating 3 time points until 4:30PM, I did 3 time points in 30 minute intervals. Hence, I expect to see less IFN-g stimulation compared to other groups'.
 * This is a table of the tubes that were generated:

Part 4: Pouring of Polyacrylamide Gel

 * Everything went smoothly after I botched up the gels the first time round. No problems for the 2nd try.


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