Endy:F2620/Specificity

Protocols & Data

 * Specificity protocols
 * Specificity data

Issues
''Unsure. It could be because of the destroy rate taking over synthesis faster? Because the interaction generally is less eficient? But I can see cognate is also going down in this experiment so I am not sure.''
 * Were there many outliers, other than the 4-AHL well? no. there aren't any others
 * 2001 review with citations for studies using AHL variants. Our data partially agrees with the literature.
 * The data are recalibrated in GFP/s/cfu to get from Od to cfu the calibration specific for the strain was used (i.e. not the one done by C. and J.) forced through zero (non-zero intercept changes function shape and produces mess)
 * Can you explain the difference between transfer.pdf and transferlh.pdf? yes. the difference is that transfer.pdf have error bars representing standard dev while transferlh.pdf has one with low/high error bars
 * How come the variability data seems to be pretty much flat over the time course, whereas the specificity data often starts high and then drops over the time course?
 * For the specificity graphs, can we get the 0 of the z-axis to be at the origin of the x-y axes? And can the maximum be the same as that on the variability graphs? yes.done