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Patrick Harrigan

TraA Display
First of all, read PMID: 9356245. You are going to make a basic part out of

Source: pOX38 You will be making a C-terminal display system out of the TraA protein from F plasmid. pOX38 is just a particular variant of the F plasmid. You should read PMID: 9356245 for background. Note that any devices containing the part will require an F+ strain for proper expression.

The sequence of TraA is available at NP_061453 in pubmed.

You can use the online tool at http://phobius.cbr.su.se/ to locate the prepro sequence.

Leucine Zipper peptide KILR
Source: Synthetic You should read PMID: 12459719. You will make a BglBrick part encoding: VKELEDKNEELLSkiYHlrNEVARLKKLVGERGGC No start, no stop, in frame.