IGEM:UC Berkeley/2006/Knockouts by Electroporation of pOX38 x pKD46


 * 50 ml of 2YT + Carb + Kan, add 5 ml started culture and (100uL)200mg/ml Arabinose.
 * Grow to OD 1.2 at 30 degrees.
 * Spin about 40 ml in 50 ml conical for 3 minutes @ 8000 rpm. (Use machine in Keasling lab that is cooled.) Dump supernatant.
 * Resuspend in 30 ml cold 10% glycerol.
 * Spin for 3 minutes @ 7500.
 * Dump supernatant.
 * Resuspend in 30 ml cold 10% glycerol. Spin 3 for 3 minutes @ 7500.
 * Dump supernatant, resuspend in remaining volume. You will not pour out all the liquid, so don't worry about leaving enough liquid to resuspend the pellet.
 * Electroporate 50 microliters cells, 1 microliter product
 * Immediately rescue with LB.
 * Rescue for 1 hour @ 37 degrees.
 * Plate