User:Tk/Notebook/MF-xfm/2008/04/16

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Transformants

 * One new transformant on the no-DNA no-BSA plate
 * The BSA and DNA transformant from Friday grew cultures in tet medium
 * No new transformants from Monday (none expected)
 * E. coli transformation showed 62 colonies, down from previously
 * probably because I electroporated at 1.5 KV rather than 1.75 KV

New tranformation tests

 * transform testing 4x and 40x concentrations with EPB (no glycerol)
 * three washes at 45 ml each
 * resuspend 50 ul of the 40x concentration in 450 ul for the 4x concentration
 * aliquot 3x 50 ul of low and high concentrations
 * Add 0, 10 ul, 30 ul of 1 M sorbitol as an adjustment to osmolarity
 * Electroporate at 1.5 KV
 * outgrowth for 1 hour at 30C
 * plate 300 ul
 * E. coli transformants done at 1.5 KV and 1.75 KV


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