IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/07/Hao Wang

0:34 The digestion reaction begins

7:46 add 0.4uL cip and 2ul buffer to the vector system

8:07 the result come out as follows:

18:03 Recycle the fragments.

19:40 Ligate the 2-4O insert to 1-18P vector:

19:48 Ligation start

23:44 Begin the transformation of the ligation plasmid