IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/23

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9.23.10
12:05 PM

PCR Verification

Primers:10 micromolar

VF2, VR, ArsR F RFC10, ArsR R RFC10 (ArsR for positive control, separate reaction without verification primers, e. coli genome as template)

Samples:   J23100B0034ArsRSDM2B0015pSB1C3 1   J23100B0034ArsRSDM2B0015pSB1C3 2   J23100B0034ArsRSDM2B0015pSB1C3 3   + control (ArsR)  - control (H2O)  

Program:

12:45 PM

ArsB PCR

Samples:   ArsB 1 </li>  ArsB 2 </li>  ArsB 3 </li>  + control (ArsR) </li>  - control (H2O) </li> </ol>

Primers: 10 micromolar

ArsBF3.0 ArsBR3.0 ArsRRFC10 ArsRFRFC10

Program:

3:25 PM

Gel



4:00 PM

glycerol stocks

900 μL culture, 900 μL 50% glycerol for J23100B0034ArsRSDM2B0015pSB1C3 1 and J23100B0034ArsRSDM2B0015pSB1C3 2.


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