Template:SBB09 19607

Roger Lowe

Intimin (refactored)
Source: E. coli strain O157:H7 For background, you should read PMID: 11717287. You will be making a part derived from the eaeA gene which encodes the Intimin protein. You can download an annotated sequence [[Media:eaeA_region.seq | here]]. You will be making a part out of just the pink regions of this file. Do not include the green prepro region.

Note: You will be sharing the reverse oligo with whoever gets the SBB09_18094 project. You should find this person and design that oligo together.

Aluminum-Binding Peptide
Source: synthetic You should read PMID: 15703906. You will make a BglBrick encoding: VPSSGPQDTRTT No start, no stop, in frame.