IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/28

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2/28/10
Got colonies on both LamB plates!

10:00 AM

PCR Per Reaction: 18.6 μL mastermix, 1 μL template,.2 μL F primer, .2 μL R primer.

Primers:

20 micromolar

VF2

VR

Samples:

ArsR SDM1

ArsR SDM2

A1

A2

B1

B2

L1 RFP 1

L1 RFP 2

L3 GFP 1

L3 GFP 2

GolS

Program:

10:50 AM

PCR Per reaction: 19.2 μL mastermix, .4 μL F primer, .4 μL R primer.

Primers:

10 micromolar

ArsBF2

ArsBR2

samples:

ArsB 48°C

ArsB 51°C

ArsB 54°C

ArsB 57°C

ArsB 60°C

ArsB 63°C

ArsB 66°C

Negative control (no primers)

Program:

1:20 PM

Gel

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