IGEM:IMPERIAL/2007/Notebook/2007-8-3

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/3 view=threemonths format=%name/%year-%month-%day weekstart=7

Preparation of Stock Solutions

 * 2x 1 litre of M9 media
 * 2x 1 litre of LB media
 * 4x 500 ml of LB media in conical flasks

Preparation of Antibiotics Plates

 * 30x LB + Amp plates
 * 30x LB + Kan plates
 * 30x LB + Amp + Kan plates
 * 50mg/ml Amplicillin solution
 * 10mg/ml Kanamycin solution

Preparation of Competent Cells
Protocols can be found at Preparation of Competent Cells in the general protocols page
 * 1) DH5alpha and XL1Blue were made electrocompetent