10X BPTE electrophoresis buffer

Purpose
Used as the electrophoresis buffer during denaturing (via glyoxal) agarose gel electrophoresis of RNA.

Components

 * 100 mM PIPES
 * 300 mM Bis-Tris
 * 10 mM EDTA

The final pH of this 10x buffer is ~6.5.

Procedure

 * 1) Prepare by adding the following to 90 ml of distilled H2O
 * 2) *3 g of PIPES (free acid)
 * 3) *6 g of Bis-Tris (free base)
 * 4) *2 ml of 0.5 M EDTA
 * 5) Treat the solution with final concentration of 0.1% DEPC for 1 hour at 37°C
 * 6) Autoclave.