User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/06/01

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Maize Endophyte Biofertilizers
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The Daily Grind

 * Describe colony morphology of the new microbes spotted on R2A
 * Mix and ligate 16S fragments from all seeds for cloning (then do the ligation overnight)
 * Cut up the unbacterialized potatoes for propagation
 * Put 10 colonies of ACC deaminase in either pSB1A2 or pDSK-GFPuv in either 1 mL of LGI or 1 mL of LGI plus 100 mM ACC. Include plasmid free E. coli as negative control.


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