Joyce:Transformation

Procedure (for E. coli XL2-Blue)

 * 1) Thaw cells
 * 2) Pipette 50 ul of cells into an ependorff
 * 3) With gentle stirring, pippette 5 ul of a 20 ul (about 100 ng? *****) ligation reaction into the ependorff
 * 4) Place the ependorff on ice for at least 30 minutes
 * 5) Heat shock the transformation mixture for 45 seconds at 42*C
 * 6) Put the transformation mixture on ice for 2 minutes
 * 7) Add 400 ul of sterile YT to the transformation mixture
 * 8) Mix gently by inverting several times
 * 9) Incubate at 37*C for at least 45 minutes
 * 10) Centrifuge at full speed for 2 minutes
 * 11) Discard 300 ul of the supernatant
 * 12) Resuspend the pellet in the remaining 150 ul
 * 13) Plate and incubate at 37*C overnight

Solutions
YT