User:Karmella Haynes/Notebook/miR Trigger/2011/06/22

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

06/22/11

 * YFP time course: repeat with other concentrations of dox

YFP/miR-luc time course > Plate JDS33-47 cells at ~1x105 in 5x 12-well plates; use U2OS plain medium > Set up three wells per time point for triplicate trials > +Dox (1μg/mL) time points:
 * Plates 1, 2: 0.1 ug/ml dox; 8 time points
 * Plates 3, 4: 0.01 ug/ml dox; 8 time points
 * Plate 5: no dox
 * 1) Wed, 6/22/11, 1 pm (55 hrs.)
 * 2) Wed, 6/22/11, 8 pm (48 hrs.)
 * 3) Thurs, 6/23/11, 8 am (36 hrs.)
 * 4) Thurs, 6/23/11, 8 pm (24 hrs.)
 * 5) Fri, 6/24/11, 8 am (12 hrs.)
 * 6) Fri, 6/24/11, 11 am (9 hrs.)
 * 7) Fri, 6/24/11, 2 pm (6 hrs.)
 * 8) Fri, 6/24/11, 5 pm (3 hrs.)
 * 9) no dox

--> Harvest cells at 8 pm for Flow Cytometry


 * }