Chang Lab:Notebook/CBE/08/149/2008/12/03

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 * style="background-color: #FFF"|   Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
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 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page


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Logs

 * Started at 1100am.
 * Culture medium
 * 14g of LB Agar weighed out on a weighing scale into a 400ml bottle.
 * 400ml of distilled water was added (2 bottles done)
 * Repeat process for LB Broth (2 bottles).
 * Autoclaved 1140am at 121 degrees for 15min (waited for about 2hrs for the tempeartue to rise and pressure to build up and lower). Temperature safe to remove is 80 degrees.
 * LB Broth was stored in the incubator at 70 degrees.
 * LB Agar was taken out to the ventilating hood and poured out into 40 petri dishes (about 15ml per dish to cover).
 * Petri dishes were parafilmed and stored in the 4degree fridge.
 * Prepared E coli (K12 W3110) broth mixture and incubated at 30 degrees.
 * E coli was filled with LB Broth in a test tube (ratio 1ml:9ml).
 * Placed in a shaking incubator at 30rpm for 16hr for 30 degrees.


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