Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-11

James Field:

Aims:

Wet lab:
 * Check plates from yesterday
 * Start cultures (5ml) for wednesday of all biobricks

Dry lab:
 * Finish colanic acid assay and SLIC cloning strategy

Results:

Wet lab:
 * Checked plates from Monday - pBAD failed to grow so re-plated. Others appeared fine.
 * Completed miniprep prep for Wednesday.

Dry lab:
 * Continued (but did not finish colanic acid assay protocol).
 * Read up on but did not design primers for SLIC.

David Roche 04:39, 11 August 2009 (EDT) Aims: Dry Lab:
 * Finish trehalose assay, and SLIC cloning strategy.
 * Make sure M2 is completely finished.

Aims:
 * Nuri Purswani 04:40, 11 August 2009 (EDT):
 * Finish CRP-glucose promoter information: Cloning strategy
 * Promoter assay

Dineka Khurmi 05:15, 11 August 2009 (EDT) Aims:


 * Finish promoter, Dam assays, and SLIC cloning strategy.
 * Make sure M3 is complete.

Kun Xue 05:15, 11 August 2009 (EDT) Aims:


 * Finish restriction enzyme, Dam assays, and SLIC cloning strategy.
 * Make sure M3 is complete.

Results:

Royah Vaezi Dry Lab Wet Lab
 * Did a RE and methylation assay, subject to approval
 * Put up a cloning strategy to fit multiple cloning advantage of SLIC
 * Looked through M1
 * Helped Dineka and Kun with M3 assays.
 * Completed PCR of OtsB - failed to obtain bands on gel so this will be re-done.