Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/12

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12 February 2009 Lab Meeting Agenda

 * Status: compressed air and vacuum? Why did the work not happen?

† Announcements † Flu R01 † SEPSIS Project †RNA project † COBRA - Direct measurement with engineered SERS substrate. - 500umx500umx50um dimension informed to Luca and Bjoern - Need to schedule when they are ready.
 * Sonali out for a week.
 * New senior project team.
 * MicroTAS abstracts.
 * Modified Schedule this week.
 * Plaque Assay.
 * Sonali done with draft.
 * More formal report to Jeff from Cathie.
 * Do cDNA prep. Spec, gels, send to Jeff B. Shipping?
 * Mark- Control circuit - done, need to be tested.
 * Integration test from here forward br>
 * Test with engineered SERS substrates

- Try glass fixture. - - - difficulty in locating the bacteria - needs much higher particle/bacteria ratio † Valve Array/Valve building (FJ) † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR † RCA † HDA † Senior project † F31: Cochlea † Silica Optimization (Lambda):
 * Substrates (JZ)
 * Colloid experiment. inconclusive. Look at SEMs
 * Teddy on track.
 * Valve tested
 * Paper drafting!
 * Final experiments in process? Need one more experiment next week
 * Final experiments in process? Need one more experiment next week
 * IRB still in revision.
 * flu cDNA　ｗａｓ　ｓｕｃｃｅｓｓｆｕｌｌｙ　ａｍｐｌｉｆｉｅｄ　ｏｎ　ｃｈｉｐ，ｏｎｅ　ｂａｎｄ　ｓｈｏｗ　ｕｐ　ｉｎ　ｇｅｌ．　ｅｘｐｅｒｉｍｅｎｔ　ｈａｓ　ｂｅｅｎ　ｒｅｐｅａｔｅｄ　ｆｏｒ　ｔｈｒｅｅ　ｔｉｍｅ．
 * ｗｏｒｋｉｎｇ　ｏｎ　ｔｈｅ　surface coating of zeonex, looking for an effective way to verify the PEG layer
 * tried contact angle, device broken
 * SEM did not give out a lot of information
 * scheduling XPS
 * DNA chain model is being made (5 beads model tested).
 * Cathie submitted One pager Whitepaper. Waiting for response.
 * Large volume filling and valve tested.
 * Design issue - whole integration or assemble parts (SPE & HDA chamber)
 * Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine
 * Pre-gent vs. Post-gent-- most interesting data: decorin upregulation, fibronectin gradient
 * Six antibodies. Verifying 4 with protein pre- and co-incubation with antibody.  Could not find the other two proteins
 * Finalized poster and printing for ARO
 * ARO is next week
 * Overview to look at the data collectively


 * Next E. coli.


 * try another color DNA dye. Pico green works ok

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html

Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


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