User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/05/30

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ABSTRACT

 * Completion of TAP media preparation. Making of phosphate buffer; filtration of Trace Elements solution and preparation of 500ml of both liquid and solid medium.


 * Today Pablo and I finished the preparation of the TAP media for Chlamydomonas reinhardtii.
 * We filter the Trace elements solution with a 50 ml syringe and filters of 22μm.
 * We prepare 0.25 Lt of phosphate buffer 1M pH 7, with this proportions:


 * Finally we mix the following proportions to make 500 ml of medium:

We prepare 500 ml of liquid medium and 500 ml of solid medium by adding 8g of agarose.

This same day we re-culture Chlamydomonas in 2 petri dishes with solid medium and in 2 tubes with liquid medium. We leave 2 controls also.