IGEM:Cambridge/2008/Notebook/Bacillus/2008/07/22

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We have 4 tubes from last year. These strains are frozen in glycerol.


 * PNZ8901 plasmid in E.coli MC1061 Strain
 * Chloroamphenicol resistant
 * "sure" vector
 * does not integrate


 * B. subtilis strain 1A1
 * No resistance
 * Same as strain 168 but tryptophan deficient


 * E.coli strain MC1061, no plasmid


 * PP182 plasmid in E.coli strain DH5alpha
 * Ampicillin resistance
 * integrates

Checking antibiotic resistance
Purpose : Grow each of them on a plate to test their antibiotic resistance

Protocol
 * Warm up frozen tubes
 * Take 15uL of each and put it on plates without antibiotic
 * Incubate at 37°C