User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/25

 .todo { color: red } .done { color: green} {| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] RII Overlay all
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Summary

 * All HEK293 Cells died, realized that it had not been 15% but 45% CSE!! Experiment failed, no repeat due to time issues.
 * Aided Caroline in her Cotransin experiment with the ASMC
 * RII Overlay results
 * Purified Rabbit IgG |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC I5006 Sigma Aldrich

Results

 * RII Overlay

Discussion

 * PKA Co-IP Appears still unsuccessful, at least RII subunits would be expected to be visible
 * Even AKAP18δ-PP seems to inhibit some RII binding, seeing as the signal is somewhat fainter compared to the untreated blots


 * }