IGEM:IMPERIAL/2009/Feedback & Debriefs/Feedback 21 7

Tuesday 21st July 2009 - Presentation to Profs. & Advisors
Prof: - need 2-3 slides focusing on this - there is a real problem/real emotional message to get across - is a novel application! Focus on one application with the method being compatible to other applications Advisor: - where it works - where the conversion occurs etc. - Need a summary after each Prof: - Have the PKU pathway Prof: Prof: - killing strategy - encapsulation Prof: - can be done - use GFP reporter with an optical read out? Advisor: Prof:
 * Need a project overview
 * Presentation does not follow the engineering process
 * PKU
 * Need details of the enzyme
 * What is the recommendation per module?!
 * More modelling
 * How to trigger encapsulation process?
 * How feasible is the killing strategy?
 * Which track are we aiming to enter?
 * How close is this to previous projects?
 * Need something that actually works with results
 * Match the thresholds and sensitivities
 * Produce 2 chassis/cells?
 * Explore module, get genes, find existing bacillus/E.coli chassis, one with a good coat formation and one that can produce the drug.
 * Create new self encapsulation module
 * Take out the biological pathway and place in Bacillus
 * Drill down
 * Make sure it’s quality controlled before consumption

Prof: - waiting time should not be a limiting factor - got bones of a good project - like idea of modules Advisor: - there is one Prof: - Use protein deficient strain? Deadline for BioBrick design: Friday Prof & Advisor: What is the critical path in the project? Prof: - where are they on they wiki? - convince what you want to pick is feasible/efficient - what are the mechanisms (existing) - why do we need the enzyme? - regulation, control, how does it know what/how much to make? - give details, details, details!
 * Be brutal in testing the risk for each module
 * Try to order parts ASAP
 * Come on a lot since Friday
 * Write a high level specification page (case study almost?!)
 * Look at an E.coli apoptotic mechanism
 * What about the proteases?!
 * Still a lot of yes/no points we need to sort out
 * Amalyse, protease, lipase deficiency?
 * Enzyme deficiencies in the gut
 * Need the pros and cons of different applications