IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/05/26

{| width="800"
 * style="background-color: #800000"|[[Image:UIUC_ArsR_System.jpg|150px]] UIUC - Arsenic Collection System!
 * style="background-color: #AFEEEE" align="center"|  |Main project page
 * style="background-color: #AFEEEE" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

5/26/10
Bootcamp Day 3

No growth observed on plates transformed with ligation products from yesterday. Re-plated 12M and 12O constructs as follows:

Incubate 10 minutes RT Inactivate 20 minutes 80°C

NB: used 5X Invitrogen ligase buffer with NEB ligase instead of 10X NEB ligase buffer which may be a problem...

Transformation

100 ul competent cells, 20 ul ligation product

electroporate

add 100 ul LB broth

37°C 1 hour recovery.

Plating

For both 12O and 12M:

100 ul on CAP

100 ul on LB

50 ul on CAP

50 ul on LB


 * }