French Lab



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Version: 22 May 2007

Projects

 * Magnetic bacteria: Jen Bell
 * Magnetic bacteria: Dzianis Trubitsyn
 * Conventional whole cell biosensors: Chris French
 * 'Bacillosensors': Chris French and Lucy Montgomery
 * Synthetic biology-based biosensors: Chris French, Xiaonan Wang, Nimisha Joshi
 * Phytoremediation: Chris French
 * Thiocyanate degradation: Chris French
 * Flavin-dependent monooxygenases: Peter Wilson
 * Bacteriohemerythrins: C. French
 * Biofilms: Gary Dorken
 * Making a broad host range BioBrick vector: Edinburgh iGEM2007 team and C. French

Outreach

 * 1. Portrayal of Scientists in Science Fiction

Workshops

 * Synthetic Biology Case Study 1: development of an arsenic biosensor
 * Synthetic Biology Case Study 2: development of processes for bioconversion of cellulosic materials

Biobrick Protocols

 * 1. Primer Design
 * 2. Cloning parts by PCR with Pfu polymerase
 * 2b. Cloning parts by PCR with Kod polymerase
 * 3. Agarose Gel Electrophoresis
 * 4. Purifying a PCR product from solution
 * 5. Cloning a PCR product into a biobrick vector
 * 6. Preparing and using competent E. coli cells
 * 7. Screening colonies by PCR
 * 8. Plasmid DNA minipreps
 * 8b. Plasmid DNA maxipreps
 * 9. Analytical restriction digests
 * 10. Combining two biobricks to make a new biobrick
 * 11. Adding a ribosome binding site to a biobrick coding sequence
 * 12. BALTIC: a new method for combining standard BioBricks
 * 13. MABEL: a fast easy method for removing unwanted restriction sites
 * 14. BABEL: a new method for generating BioBricks without restriction digests.

Other Protocols

 * Growth of E. coli
 * Growth of B. subtilis
 * Transforming Bacillus subtilis
 * Plasmid DNA minipreps
 * Restriction digests
 * Agarose gel electrophoresis
 * PCR
 * Purification of DNA fragments
 * Ligation
 * Cloning of PCR products
 * Preparation of cell extracts
 * Protein Assay
 * SDS-PAGE
 * Native-PAGE
 * Enrichment and growth of environmental bacteria
 * Identification of bacteria
 * Gram stain
 * Making glycerol stocks
 * Assaying polysaccharides with the anthrone assay

Lab information
Catalog of primers

Safety Information
Hazardous chemicals list

Other links
C. French main page

Bacteriology page

SBS page

University of Edinburgh.


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