Sauer:Davis Minimal Media

Davis Minimal Media (10x) 1 L:

K2HPO4 - 90 gr

KH2PO4 - 30 gr

(NH4)SO4 - 20 gr

NaCitrate - 5 gr

MgSO4100x (100gr/L) (1%)

Glucose100x 20%

vitamin B1 (thiamine)2000x 2 mg/mL

Amino Acids200x 10 mg/mL

Plates (1 L)

800 mL H20 ddw + 20 gr agar --> autoclave (in hebrew)

Davis10x 100 mL

Glucose 10 mL

1% MgSO4 10 mL

Antibiotic weighted

DDW 80 mL Sterile