Picking Colonies

Picking Colonies (4/6) based on S. Boyarskiy's protocol:
 * Materials needed:
 * 5mL (Note: that's milli, not &micro;) pipette -- in the JCA lab, this can be found in cabinets in rear of room. Return after use.
 * Use LB with antibiotic necessary for your experiment (Spec in our case for toxR plasmids)
 * 1 test tube, labeled
 * 1 bunsen burner (to sterilize pipette tip before drawing 5mL of antibiotic-LB, tube cap)
 * Do NOT breach the Z-axis of the burner's flame when grabbing materials on bench top/counter space.
 * toothpick to pick colony.


 * Tips for picking colonies
 * Pick an average colony (Not the biggest or smallest).
 * Make sure it is not in contact with any other colonies.
 * After putting 5mL of antibiotic-LB mixture into tube, lightly press toothpick into selected colony and drop into test tube.


 * Incubating picked colony in Warm Room (448A Stanley)
 * Turn off shaker before placing tube into rack.
 * Make sure to turn the shaker back on before you leave, don't F other's experiments up.
 * Turn light to room off after you are done. (On wall in hallway)