User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/07/02

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 * width="500pt" align="center" style="background-color:#EEE"| |T7 RNAP transcription of rRNA
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 * Obtain detection substrate from Bryan
 * Order chemifluorescent detection substrate.
 * Research in vitro transcription to design better standards.

Primer design information
>wt AGGGGAACCTGCGGTTGGATCACCTCCTTA

>mutant AGGGGAACCTGCGGTTGGATCATTGTGGTA

wt16sProbe1 wt16sProbe2
 * 5'-GGT TGG ATC ACC TCC-3' 49
 * 5'-GTT GGA TCA CCT CCT TA-3' 48
 * reverse complement - TAAGGAGGTGATCCAAC

o16sProbe1 o16sProbe2
 * 5'-GTT GGA TCA TTG TGG-3' 44
 * 5'-GTT GGA TCA TTG TGG TTA-3' 47 - ordered this
 * reverse complement - TAACCACAATGATCCAAC
 * reverse complement - TACCACAATGATCCAAC - ordered this


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