User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/10

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Gravin Co-IP gel
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Destaining gel remains that was coomassie stained after blotting the cAMP precipitation samples
 * Putting the AKAP250 immunoprecipitation samples to gel and blot them
 * Gradient (4-12%) SDS-PAGE (NuPage) for AKAP250 Co-IP Precipitations
 * After this gel has been blot it will be blocked and then together with the other blot treated with antiAKAP250 antibody

Results

 * Coomassie staining was put together with the ponceau S staining of yesterday
 * Ponceau staining of AKAP250 Co-IP blot

Discussion

 * Very peculiar that the Ponceau staining gives the exact same image as the cAMP precipitations.


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