IGEM:Peking University/2008/Experiment/PKU 0717 G2 1

PKU_080717_G2_1

 * Screen for pGREF505-lexO clonies


 * Plasmid Miniprep-G4M1278-13~24
 * Sequencing-G4M1278-14/17/24


 * Result for last sequencing: G4M1278-1&2 are right point mutations.


 * Point mutation-T1278G/T1743G
 * Using right wtGAL4 TG-11


 * Primer Name:T1743G-F & T1743G-R


 * PCR system


 * conditions


 * Result: have about 7000 band, waiting for sequencing check


 * Digestion-point muation product-overnight/4°C