IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/31

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Tuesday 31st August:

 * Many overnight restriction digests were set up as shown bellow:

1) pACYC   Plasmid - 35μL   Nco1  - 1μL   Buffer 3 - 4μL   BSA (x100) - 0.4μL

2) pSB1C3   Plasmid- 10μL   Pst1 - 2μL   EcoR1 - 2μL   EcoR1 buffer - 2μL   BSA (x20) - 1μL   H2O - 3μL

3) pga Promoter   Plasmid - 10μL   EcoR1 - 2μL   EcoR1 buffer - 2μL   Spe1 - 2μL   BSA (x20) - 1μL   H2O - 3μL

4) csrB   Plasmid - 10μL   EcoR1 - 2μL   EcoR1 buffer - 2μL   Pst1 - 2μL   BSA (x20) - 1μL   H2O - 3μL

5) Part BBa_E0040 (GFP)  Plasmid - 10μL   EcoR1 - 2μL   Spe1 - 2μL   EcoR1 buffer - 2μL   BSA (x20) - 1μL   H2O - 3μL


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