IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/06

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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July 6, 2010

 * Autoclaved glassware
 * Made Terrific Broth without antibiotics
 * Grew out CFP, YFP, and GFP
 * Did a transformation of RFP that has a tetracycline resistance (RFP biobrick into TOP10 cells)
 * Made solutions of copper solutions and TOP10 cells to determine optimal growth environment
 * Analyzed alignment utilizing BLAST of construct templates and primer sequences
 * Transformed YFP biobrick into TOP10 cells
 * Grew out YFP, CFP, and YFP from glycerol stock for testing of fluorescence using the plate reader in the morning
 * Contacted team about next regional meeting
 * Discussed skype calls and established set individuals on each team that would be in charge of maintaining communication over skype


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