Melee:20.109 stuff

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Module 4 Research Proposal
melee:mod4_proposal

SAGA Subunits, S. cerevisiae
melee:saga_expt

M13 Refactoring
I attempted to remove all overlapping sites in this section of the genome. What I did was copy the overlapping section (usually an RBS and/or promoter overlapping the previous ORF) and repeat it. I changed the wobble positions of every other codon on the ORF’s so that they wouldn’t homologously recombine with the repeated sequence. Additionally, as was done on the T7.1 refactoring, I added two new cut sites between the separated genes. I chose to keep the overlap between the gene VIII promoter and gene IX RBS (and the resulting mixed order of genetic elements) changing that order seemed to be a much more significant change than the others.

The refactored genome is Part:BBa_M31511 on the Registry of Standard Biological Parts