IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/30

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Team Allergy
LTP ihpRNA parts that worked: 9c2, 11c1, 11c2
 * Sequencing Results:

Bet 2 ihpRNA parts that worked: 25c1,c2, 28c1,(LTPS+PDK is actually Bet 2 hpRNA)

Ger ihpRNA parts that worked: 36c1,c2, 37c1,2, 38c1,c2

Team Flavor
Ligations were plated and left @ 37°C O/N.
 * Ligated StrepII tagged proteins into V24 backbone
 * Ligated cut J45004 into V0120 backbone

PCR again for VP16
Using pfx polymerase
 * 1μL 1/10 dilution Hind.Fwd
 * 1μL 1/10 dilution VP16.Rev
 * 1μL DNTPs
 * 6μL enhancer buffer
 * .5μL MgSO4
 * .5μL PFX polymerase
 * 1μL 1/10 dilution VP16 #1
 * 5μL DH2O
 * 4μL amp(lification?) buffer

Running PCR on two reactions. There was not enough PFX for both to get .5μL, so only one of them is likely to actually amplify.

Second ACC + RxrHm digest gel


yes to ACC (left) no RxrHm