Buckner Lab:Notebook/Summer 2010/2010/05/19

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] DNA Purification and Gel Electrophoresis
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Plasmid Isolation

 * Used QIAprep isolation kit from Qiagen to isolate plasmids.
 * Performed EcoR1 digestion to isolated DNA for ~2.25 hrs

Pouring and Running Gel

 * Made a 0.8% agarose gel with ethidium bromide
 * Ran the gel for 1.5 hrs in TAE buffer at 60V.
 * Photographed and annotated gels

-Didn't allow the gel to run long enough. -Need to add all of the DNA from the prep tube when taking a "look" after an EcoRI digestion

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