User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/12

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] AKAP250 (scbt) immunoblot AKAP250 Co-IP
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Washing the AKAP250 CO-IP Blot and adding secondary antibody
 * Adding AKAP250 antibody ZDE10030 animal 122 antibody (Eurogentech antibody service) to cAMP precipitation blot blocked in blocking buffer + 1 μL/mL Azide (20%stock)

Results

 * Immunoblot for AKAP250 on AKAP250 co-ip western blot

Discussion

 * Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2
 * Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on the ponceau of this membrane


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