User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/28

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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WetLab

 * Plasmid extraction again! of DH5α, this time trying to get better concentration.
 * Roche´s kit. Elution buffer: just 40μL.

Picture not available.
 * Electrophoresis gel of:
 * PCR product with 80V, 90min, 2%.
 * Today's plasmid extraction. 80V, 90 min, 1%.

Digestion

 * 4 double (XbaI & HindIII) digestions were performed, with Daniel´s extraction and mine extracted today.

†Daniel's extraction ‡Fabricio's extraction