User:Tk/Notebook/MF/2008/07/31

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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Transposon insertion event at 523000-525000
A pseudogene and inactive IS3/IS1138 transposon is at 523336-524543


 * IRL: 523336-523356
 * IRR: 524523-524543
 * The sequence of the transposon insertion site is: ataaactgggaaaaataaaat / ataaactgggaaaaaataaat
 * Compare to the IS1138 transposon end sequences: taaactgggacaaaaaaat / taaactaggacaaaaaaact


 * Transposase gene has three frame shifts and multiple stop codons (both TAA and TAG)
 * Approximate locations:
 * 523660-524485
 * Frame 1: 523660-523993
 * Frame 3: 523969-524161
 * Frame 2: 524170-524260
 * Frame 3: 524275-524485


 * There may be a six bp repeat outside of the IRL/IRR boundary:
 * taagca on the left
 * gatgca on the right


 * Tn5 transposon insertion events captured:
 * TT01-3111_TT01-.seq	524040	fwd
 * TT01-3233_TT01-.seq	524201	rev
 * TT01-2677_TT01-.seq	524358	fwd
 * TT01-3383_TT01-.seq	524358	fwd


 * Vector NTI file of the 521K-526K region [[media:MF521K-526K.gb]]


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