IGEM:PennState/2008/PreparingElectrocompetentCells

=Electrocompetent Cell Prep=

Starter Culture

 * Inoculate desired cell strain (DH5a) into 3 mL of LB and shake at 37C

Prep

 * 1) OD the starter culture, use to calculate the dilution into 30 mL of LB for desired growth time (~30 min doubling time).
 * 2) Shake in falcon tubes at 37C until OD of 0.5-0.6
 * 3) Put cells in ice water bath for 30 min
 * 4) Wash Cells
 * 5) Spin down cells in rotor (max rpm)at 4C for 10 min
 * 6) Pour off supernate and re suspend cells in 30mL of cold water
 * 7) Repeat wash 2 times
 * 8) After final spin down, pour off the supernate and re suspend cells in ~300uL water
 * 9) Use 70uL allloquats for transformations