User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/04

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Inoculated Liquid culture with yeast from slants
NCYC 2827, 2878, 415, 971 and 814

1) 10mL Glucose+Yeast Media broth with sterile toothpick 2) Incubate at 30C

QIAquick PCR purification and Cycle Sequencing
Clean S. Dairenenses with QIAquick 4.9ng/ul