IGEM:Peking/2007/Switch-Notebook/2007-7-19

Experimenter: CXY, CCY

Colony PCR test of SS1-pMD18T-3, CD1-pMD18T-3
Plasmids are diluted to 1/50 of concentration before PCR. OK!! Incubate for Miniprep and digestion test tomorrow.
 * Primers: Sul_S_1_F, Sul_S_1_R
 * Enzyme: Easy Taq

Colony PCR test of pLX007/8/9-lacZa

 * Primers: test_F_new, test_R
 * Enzyme: Easy Taq



pLX007-lacZa(1-21), pLX007 - Control, Marker, pLX008-lacZa(22-23)



pLX008-lacZa(24-41), Marker, pLX008-lacZa(42-47)



pLX008-lacZa(48-66), pLX008 – Control, Marker, pLX009-lacZa(67-70)



pLX009-lacZa(71-93), pLX009 – Control, Marker

pLX007-lacZa(8), pLX008-lacZa(40,41), pLX009-lacZa(68,88) OK!! Incubate for Miniprep and digestion test tomorrow.