User:Andy Maloney/Kinesin & Microtubule Page/PEM-G

PEM
See the above page for more information.

|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC GTP
Originally we purchased GTP from Cytoskeleton. Unfortunately, Cytoskeleton doesn't tell us what exactly it is we purchased and they have mixed protocols about how to suspend GTP into solution.

Since we have a salt form of GTP, it goes into solution very easily. I have it stored in a secondary container that is filled with desiccant and under a nitrogen environment in the -20˚C freezer. I also wrapped Parafilm around the cap to ensure that no moisture gets in.

Recipe

 * PEM
 * 100 mM |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC GTP
 * 1 mM |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC MgCl2