Registry/Measurement kit/Notebook/2007-7-6

T9002

 * Ligated sequential and double digests of T9002 to 3K3
 * Transforming in MG1655

E0240

 * Re-doing BB PCR with vent and phusion
 * Mix for rxn (100uL):
 * 1) 8uL 2.5mM dNTP
 * 2) 20uL phusion buffer
 * 3) 1.5uL f primer
 * 4) 1.5uL r primer
 * 5) 1uL DNA
 * 6) 67uL H2O


 * Just realized that we skipped the ligation step completely (3K3 and 1AK3).
 * Doing that now, discarding PCR product.

I2056

 * Re-culturing from plate

I2055

 * Checked the sequences; none of them have the promoter.
 * Waiting for primers to insert the promoter.