User:Tk/Notebook/MF/2008/11/04

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
 * style="background-color: #F2F2F2" align="center"|  |Main Page
 * style="background-color: #F2F2F2" align="center"|  |Main Page


 * colspan="2"|
 * colspan="2"|

Part construction

 * Miniprep of four cultures each for J70027, J70024, J70025
 * Cut J70027, J70025 with E-S
 * Cut J70024 with X-P
 * Ran 5 ul on an E-Gel


 * Lanes: marker, J70027ABC, J70024ABC, J70025ABC, J70027D, marker



Choose J70027A, J70024A, J70025A

Assemble Tet cassette
J70028 (Kan) = J70025 (CM) + J70024 (CM): this should be tet resistant also


 * Four versions, J70025A/C with J70024A/C
 * AA, AC, CA, CC

Sequence new components
Sequence with VF2, VR:


 * J70027A,D
 * J70024A,C
 * J70025A,C


 * }