User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/10

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October 10th, 2010
1. Make XbaI-PstI double restriction to ΔRBS + GFP E004 Pure PCR product.
 * XbaI-PstI double restriction methods:
 * DNA -> 5 ul
 * Buffer 2 -> 2 ul (10% of total volume)
 * BSA -> 1 ul
 * XbaI -> 1 ul
 * PstI -> 1 ul
 * HPLC -> 10 ul (to complete total volume of 20ul)
 * Incubate at 37º C overnight


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