IGEM:IMPERIAL/2007/Experimental Design/Phase2/Results 1.2

Result Summary

 * Tests were carried out on the following concentrations of GFP:
 * 3.7&micro;M, 1.8&micro;M, 1&micro;M, 0.5&micro;M, 0.1&micro;M, 0.05&micro;M, 0.025&micro;M, 0.0125&micro;M and 0&micro;M

The experiment was carried out to calibrate the response of GFP fluorescence to different concentrations of GFP, in-vitro. [GFP] and fluorescence would be expected to be directly proportional as they have a linear relation at certain concentrations.

The results obtained correlate with what was expected as the graph of fluorescence against [GFP] is a sraight line through the origin. The gradient of this graph is almost 1.5 times the gradient for GFP in-vivo, as seen in the characterisation of part F2620. This means that a particular concentration of GFP in-vitro has 1.5 times more fluorescence than the same concentration of GFP in-vivo.

Complete set of results can be found here:


 * [[Media:IC 2007 Calibration Curve.xls|Preliminary test ]]
 * [[Media:IC 2007 Calibration Curve2.xls|Second test ]]