IGEM:Caltech/2008/Project/Tasks

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ROS Bursts

 * Test catalase deficient strains
 * Test other oxidases

Integrated Prophage

 * Design/order lamB primers
 * Find spo2 sticky ends
 * Pick up a wt subtilis strain (Elowitz Lab) and find a good promoter
 * Choose a selectable lysogenic phage
 * Coli→subtilis conjugation?
 * Constitutive recA
 * Need to order primers
 * Figure out transformation protocol for 50kb plasmid

Population Variation

 * Primers to amplify promoter with different 5' lengths
 * fimE plasmid (Keasling/Arkin labs)
 * Primers to BioBrick fimE
 * Find fimE binding sequence
 * Order binding site oligos (new BioBrick parts)
 * Natural SSM examples
 * And order primers

Vitamins

 * Primers to amplify lactis folate enzymes
 * Do lactis RBS's work in coli?

Lactose Intolerance

 * Primers to amplify and mutate lacY
 * lacY+ strain (for a lacY template)
 * Get, then BioBrick holin/lysozyme genes


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