Freimoser:Protocols:ProteinQuantification

Source
The quantification methods listed below represent those that we find most useful. The descriptions are part of a more detailed list of protein quantification methods General Reference: Stoscheck, CM. Quantitation of Protein. Methods in Enzymology 182: 50-69 (1990). 

Absorbance at 280 nm

 * Range: 20 μg to 3 mg
 * Volume: Depends on cuvette - volumes range from 200 microliters to 3 ml or greater
 * Accuracy: Fair
 * Convenience: Excellent, if equipment available
 * Major interfering agents: Detergents, nucleic acids, particulates, lipid droplets

Absorbance at 205 nm

 * Range: Roughly 1 to 100 μg
 * Volume: Depends on cuvette - volumes range from 200 microliters to 3 ml or greater
 * Accuracy: Fair
 * Convenience: Very good
 * Major interfering agents: Detergents, nucleic acids, particulates, lipid droplets

Modified Lowry

 * Range: 2 to 100 μg
 * Volume: 1 ml (scale up for larger cuvettes)
 * Accuracy: Good
 * Convenience: Fair
 * Major interfering agents: Strong acids, ammonium sulfate

Bradford assay

 * Range: 1 to 20 μg (micro assay); 20 to 200 μg (macro assay)
 * Volume: 1 ml (micro); 5.5 ml (macro)
 * Accuracy: Good
 * Convenience: Excellent
 * Major interfering agents: None