IGEM:Peking/2007/Switch-Notebook/2007-8-10

rescue by precipitation
9 fragments: lac, rec, sul, SS1-3, SD1-3, digested by XhoI/SpeI

1 vector: GFP-plx007, digested by XhoI/XbaI

miniprep
T1TE-plx007

T1TE-T1T2-pcc010

double digestion test applied to these two plasmids

sequencing results
change the primers to ensure the right result!

transformation
Prec, Plac, Psul, SS1-3,SD1-3 ligated with GFP-plx007 for 5 hours

SDY-EGFP-plx007 positive transformation

re-colony PCR of T1TE-T1T2-pcc010
forget to make the positive control! failed.