IGEM:Peking/2007/Switch-Notebook/2007-7-16

Experimenter: NM & LCB

Colony PCR of CD2up/down-pMD18T

 * Primers: MBF/R

Select for incubation & sequencing: 2,4,7,8.

Miniprep of SD, lacZa, pCC002, A-H, Y, cro
Double digestion test of BamHI, XhoI (plasmid: 10uL, digestion time: 2.5hrs)

Temperature gradient PCR of CD3

 * template: CD1-pMD18T 1uL
 * buffer: 5uL
 * dNTP: 5uL
 * F: Con_D_3_F 1uL
 * R: Rec_S_3_R 1uL
 * Extaq: 0.25uL
 * H2O: 37uL
 * temperature: 50.3, 53.2, 58.1, 63.5, 68.1

Single digestion test of pLX008(2uL), pLX009(4uL)

 * digestion test: 2.5hr