Davidson:Ligations

=Cloning= We have been able to successfully clone the following antibiotic pancakes
 * Hin
 * Hin with LVA degredation tag
 * Cmr- forward
 * Kan-forward
 * Tet-forward
 * Tet-backwards
 * Cmr-backwards

KanB =RE and Hix= We have been able to clone RE into cells. The RE that we are using has two point mutations but they should not compromise the activity of the Fis-binding sites please see the page for part Part BBa_J3101  for further details.
 * Kan backwards is the only gene that we are completely positive has not been cloned into a plasmid in the correct orientation.


 * As per the | assembly plan we will be attaching the RE downstream of the double terminator BBa_B0015


 * We also have two cultures with the correct sequences for Hix C.
 * We have sent a "Master Plate" with cultures of all of the things that we have cloned to the Mammoths.

=Cloning Parts of the Constructs=

Please see the Construction Intermediates page for a list of the ligated parts and their part numbers.