Endy:Screening plasmid/v2.0/Construction

Screening plasmid 2.0 construction has two components:
 * 1) Direct synthesis of the I/O reporter section
 * 2) *In progress - order placed 8/25/06
 * 3) Construction of several induction system 'front ends'

=Front End Construction= Construction steps:
 * 1) Build induction system from BB parts, clone into 3K3, 1A3, 2K3?
 * 2) Cap forward BB site

AHL-induced

 * 1) F2620 Ptet controls luxR expression
 * 2) F2621 lux Pl control luxR expression
 * 3) Reversed I13212 + R0062

Next Steps

 * 1) Order primers to flip I13212
 * 2) Move F2620 & F2621 into psb1a3, psb3k3, and psb4a3
 * 3) Cap E/X for F2620 & F2621 in each plasmid.
 * 4) Constructed reversed I13212, put it in plasmids, and cap E/X

IPTG-induced

 * 1) Do we have (promoter)(rbs)(lacI)(Plac) already in registry?
 * 2) *Nope we don't even have lacI without the ssrA tag, C0013 is a designed part w/o the tag, but it wasn't constructed. Though caitlin said she had ordered the primers for removing the LVA tag at one point.
 * 3) **"As for the primers, I'm not sure if they're still around. You can look in the "primers" binder above my old desk to find the name and sequence I used. It probably would have been "C0013_build_F" and "C0013_build_R". There are a few boxes of primers in the -20. (But that set might have gotten tossed...)"
 * 4) Reverse lacI

Arabinose-induced

 * 1) Do we have the right intermediate?
 * 2) * P1010
 * 3) * I13458 + I13453
 * 4) **We could build this out just to have a split up version of P1010 (would be a useful registry part).