User:Howard Boland/Notebook/Art from Synthetic Biology/2010/09/15

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End Overnight Growth pUA66katE
All samples grew but I did a late pickup.
 * 1) The samples were left in 4C, for 2 hours
 * 2) Glycerol stock samples were taken
 * 3) The remaining cells in each of four bottles (10ml) centrifuged for 10 minutes.
 * 4) The supernatant was removed
 * 5) The four bottles stored at -20C.

Start Overnight Growth pUA66kat
Two samples were grown based on the new plates transformed with confirmed pUA66katE plasmids.
 * 1) Two colonies where picked from the plates
 * 2) Inoculated each colony in 10ml LB-broth containing (10ul 50mg/ul Kanamycin)
 * 3) The samples where placed in a shaker for overnight growth.


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