IGEM:PennState/2006/Transformation

=Transformation=

Media/Reagents

 * Competent cells
 * SOC1
 * Antibiotic LB plates

Pre

 * Add plasmid DNA to alloquats from electrocompetent cell prep
 * Transfer DNA and Cells into electroporator cuvettes

Electroporation

 * 1) Shock Cells, monitor discharge time
 * 2) Add SOC to cuvettes (~400)
 * 3) Transfer to culture tubes, shake at 37 for 1 hour (depending on antibiotic marker)
 * 4) Plate ~200uL of SOC/Cells onto agar plate

1To make SOC, add 20 μL 500 mM filter-sterilized glucose to 500 uL SOB (20 mM final glucose concentration)