Kafatos:Making 1kb+ Ladder Stock

Overview
Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots

Materials

 * 1) 250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)
 * 2) 500uL 5x DNA Loading Buffer
 * 3) 1750uL AccuGene Water (Lonza)

Procedure

 * 1) Add the 3 components above together
 * 2) Mix well and make 5 x 500uL aliquots
 * 3) Leave one working stock at 4C and keep the other frozen at -20C
 * 4) Use 10uL of this stock per lane (1ug of ladder per lane)

Contact

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