User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/07/27

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=Wet Lab: OmpF/C promoters characterization=

PSB3K3

 * PCR positive control
 * FWD prefix 5μM
 * REV sufix 5μM


 * PCR OmpF
 * FWD sufix 5μM
 * REV 4136 5μM

PSB1T3

 * PCR positive control
 * FWD prefix 5μM
 * REV sufix 5μM


 * PCR OmpF
 * FWD sufix 5μM
 * REV 4136 5μM

PCR's Programme

 * 94ºC, 5:00


 * 35x
 * 94ºC, 0:45
 * 55ºC, 0:45
 * 72ºC, 3:00


 * 94ºC, 10:00
 * 4ºC, ...


 * }