Springer Lab: Antibiotics

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Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).

=G418=


 * Working concentration 200 mg/mL (1000 x)

http://openwetware.org/wiki/G418

=Clonat=


 * Working concentration 100mg/mL (1000x)

http://openwetware.org/wiki/ClonNat

Experimental

 * stock solution: 200mg/mL (1000X), stored at -20 C
 * working concentration: working concentration depends on organism and purpose of antibiotic application; stable at RT


 * yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.


 * bacteria selection: 8-16 µg/ml
 * mammalian cell maintenance: 200 µg/ml
 * mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)

Description

 * Gentamycin analog, used to select for the KanMX marker in yeast.
 * mode of action: irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
 * mode of resistance: neo/kan gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin and kanamycin by phosphorylation.

Links

 * related antibiotics: kanamycin, neomycin (all aminoglycosides)
 * FAQ on G418
 * list of cell culture material by company