User:Wayne G. Shreffler/Notebook/Daily Log/2009/07/06

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*Wayne G. Shreffler 19:24, 6 July 2009 (EDT):
Research Overview


 * Reviewed Caitlin's src family inhibition data -- both experiments do demonstrate that the inhibitors block CD63 and can be washed out
 * first experiment does not support hypothesis that src inhibition blocks anergy -- however, response is very weak
 * second experiment inconclusive as there is some 'carry over' activation from stim 1 -- will extend time between stim 1 and 2 to 30 min
 * third experiment inconclusive due to weak response
 * Will try src inhibitor again -- consider using fresh donor (?Carl)


 * Carl has separated HMW and LMW components of fraction 5, and Bert with Galina separated fractions w/o blue staining
 * Bert has now screened the activity of these fractions and tentatively finds that the HMW fraction has the activity
 * Carl will run gel tomorrow to confirm separation and obtain material to be sent for MS/MS


 * Steven and Neisha confirmed good transfection efficiency with the GFP control plasmid
 * doing transfection tomorrow


 * Alex has acquired images from anti-IgE, fMLP and control (RPMI) stimulated cells -- no evidence of nanotubes
 * will repeat at least once more
 * will acquire images from RPMI stim to compare with anti-E to confirm changes of Ag localization
 * order phalloidin and DNA stain


 * Shashi has not heard back from IRB
 * will call the office this afternoon -- can Yvette help?


 * Need to plan dinner for CW, AS, MP -- Thu night?

Completed 'to do's'


 * DoD grant reviews

To do:


 * R03 progress reports
 * Peds review
 * contact Alkis re: ICATA
 * review Duke basophil samples with Caitlin
 * JAX IRB docs


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