User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/14/2010/06/15

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

June 15th, 2010
While looking the items we received from Edimburgh we found that some primers, those for luxCDE, reverse and forward, were supposedly for LuxCDE from Photorhabdus luminescence but actually the primers were designed for LuxCDE from Photobacterium phosphoreum. Then I made a PCR to get LuxAB from genomic DNA of Vibrio fischeri MJ11 following the next procedure:

5μL Buffer PCR

2.5μL MgCl2

2.5μL DNTPs

2.5μL Preffix

2.5μL Suffix

2μL Template DNA

1μL Taq Polymerase

32μL H2O


 * }