Template:SBB10 ConstructionFiles DanielS sbb39

PCR DS003 and DS004 on pBjh1601CK-Bjh1380 (311 bp, EcoRI/BamHI) Sub into pBjk2741           (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb39         {PmgtCB}

DS001  Forward oligo for cloning of PmgtCB ccataGAATTCtccAGATCTtacggcggcaatcaggacgt DS002  Reverse oligo for cloning of PmgtCB gctgactataataagcgcaaattcaGGATCCatcag



JCA Notes
Eco/Bam transfer pBjh1601CK-Bjh1380 Sub into pBjk2741-Bca1144           (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-Bjh1380   {PmgtCB}
 * Correct, but you can do this one by eco/bam transfer
 * Need to put part into Clotho -- call it Bjh1380, not sbb39