Keck Subbasement:Inventory Aug 2010

This inventory is intended to give a full accounting of durable objects in Keck 040 and a general sense for what consumables are available in Keck, conducted on Aug. 18 by JTM. Other inventories are / will be listed here.

Northern Bench

 * Equipment
 * Eppendorf Centrifuge 5810R
 * Rotor for 30 small 1-2mL tubes
 * Large rotor with inserts up to 5x 50mL tube
 * Tools for changing out rotor etc. in cabinets below
 * Pelton & Crane Small Autoclave (working?)
 * Millipore Direct-Q 3 water purifier
 * Alarm clock
 * Eppendorf Vacufuge Plus
 * 2x sets of Eppendorf reference pipettes
 * Tiny benchtop centrifuge
 * Bio-Rad BR-2000 vortexer
 * Bio-Rad MJ Mini PCR thermocycler w/ 48 well block (can do thermal gradients)
 * Thermo Scientific Nanodrop 2000C
 * Elmer-Perkin Victor X3 2030 Multilabel Plate reader + Dell Vostro 1000 Laptop
 * Small Freezer
 * 3x 500 mL glass bottles with caps
 * 2x 250 mL erlenmeyer flasks
 * Pipette tips, falcon tubes, eppendorf tubes, and 96 well plates
 * Paraflim
 * Sink, drying racks, detergents etc.
 * Fluorimeter manuals, quartz cuvettes, cleaning supplies, and replacement lamps
 * Scale manual, accessories, weighing boats
 * Box of 15 mL glass tubes with plastic tops
 * Binders, pens


 * Chemicals & Kits
 * Qiagen PCR Purification Kits
 * Promega Wizard Plus SV Miniprep kit
 * Ultrapure Agarose
 * Glycerol
 * Urea
 * Ammonium Persulfate
 * LiCl
 * MgCl * 6 H2O
 * EtBr
 * Ethylene Glycol
 * Malachite Green oxalate
 * KCl
 * Mg acetate * 4 H20
 * Tris-EDTA 10x
 * Theophylline

Western Bench

 * Equipment
 * Bio-Rad BR-2000 Vortexer
 * Corning PC-420D hot plate / stirrer
 * Tiny benchtop centrifuge
 * VWR Standard Heatblock (fits 24 eppendorf tubes)
 * Fisher Scientific Isotemp 2150 Nano
 * UV lamp (365 nm & white light)
 * Mettler-Toledo AB54-S/Fact Scale (10mg to 50g)
 * Pipette + charger
 * Labnet International Enduro Power Supply 300V
 * Fisher Scientific FB300 Power Supply
 * Two large gel boxes + four gel trays + many combs
 * Cell SureLock PAGE gel stand
 * Chemical Hood
 * Fridge
 * Tilt Box
 * Unknown pump (looks like its an aquarium pump)
 * Unknown glass/metal tube (four attachments, gas tube sealed in metal frame, possibly electrical linkages on top?)
 * Vacuum pump (under chemical hood)


 * Chemicals & Kits
 * 2x boxes of Qiagen gel extraction kits, not dated
 * 4x boxes of Qiagen miniprep kits, two dated to 2008
 * 10X TBE
 * 10X TAE
 * 200 proof EtOH USP grade (in cabinet under hood)
 * 1x Bonner's Salt
 * kanamycin sulfate


 * Misc
 * Al foil
 * Gloves
 * Pipettes (10ml to 100ml)
 * Saran wrap

Southern Wall

 * Equipment
 * Microwave
 * Empty cabinet with gas tank attachments
 * Alpha Innotech Mini Gel Imager (302nm & 365nm) + Thermal printer & computer
 * Thermo Scientific Revco Value Plus -80C
 * Dell 17" LCD screen, unattached to a computer
 * Xerox Phaser 6100 Printer (dsdna)
 * Toolkit with wrenches & tubing
 * Three inkjet printers, unconnected to anything

Fluorimeter Room

 * Equipment
 * Horibajobinyvon Fluorolog 3 with computer, thermal management
 * Bio-Rad BR-2000 Vortexer
 * Tiny benchtop centrifuge
 * Large empty crate


 * Chemicals
 * n-Hexadecane
 * Silicone oil

The Island

 * Equipment
 * Pixma MP210 Scanner/Printer
 * UV Faceshield, good condition
 * Thermo Scientific MaxQ Shaker (heating only)
 * Eppendorf 5418 Centrifuge with 18 eppendorf rotor
 * Power outlet from ceiling

The Freezer

 * EtOH
 * rhodamine phalloidin
 * Adenosine-3', 5'cyclic monophosphate sodium salt
 * 2x Monoclonal antitubulin polyglutmylated antibody
 * 2x Taq polymerase (expiring 6/2011)
 * DAPI
 * Sybr Gold
 * 1kb and 100bp ladders
 * Phusion sample (assayed 07/09 - possibly empty)
 * Phire Hot Start PCR Sample (assayed 07/09 - almost empty)
 * Glycogen (Ambion)
 * 50x gel buffer II tubes (Ambion
 * T7 RNA Polymerase 300k U
 * 8x PCR dNTP mix 10mM (expiring 9/2010)
 * Many tubes of TURBO DNase (in MEGAshortscript T7 boxes, dated 4/22/09, 6/15/09, 8/14/09, and 10/23/09 respectively)
 * 2x MEGAshortscript T7 boxes
 * Cold packs
 * Plastic tube rack with 25 cryptically labeled tubes of clear fluid/nothing
 * Blue Keep-Cold Freezer Box
 * Pureexpress solutions
 * RNase H
 * Pyrophosphatase
 * Ribonuclease R
 * T4 DNA Ligase (expiring 7/2010)
 * T4 DNA Ligase buffer (expiring 8/2011)
 * T7 enzyme mixes
 * T7 RNA Polymerase
 * Bags of tubes
 * "Arjun's Plasmids"
 * "EPIBIO Buf & DTT 5-2010"
 * "Elisa EPIBIO TXN KIT STOCK 6-10-2009"
 * Tubes of DNA from IDT to "P. Forsburg"


 * Freezer Boxes
 * "Elisa PFRR 2008-2010"
 * "FEI"
 * "Elisa Franco"
 * "Elisa TXN"
 * "Bistable circuit"
 * "EF Rybozymes"
 * "Elisa oscillator for patterns"
 * "RNA Elisa"
 * "Elisa NFRR V2"
 * "DNA for beads / tweezers Elisa 9/24/2008 8/25/2009"
 * "Roman DNA-box"
 * "EF OSC SEP 09"
 * Unlabeled with many multicolored tubes
 * Unlabeled with a few tubes with loading buffer colored droplets in them

The Fridge

 * 38% acrylamide/2% bis stock (Ambion)
 * Anti-mouse IgM FITC (targets /mu chains)
 * TEMED
 * Precast HEPES & TBE gels
 * Bradford assay reagent (no protein standards found to go with)
 * Unlabeled silver canister, tightly sealed, bagged, taped, and cable-tied
 * Two empty styrofoam boxes
 * One possibly unopened styrofoam box from Invitrogen to Elisa Franco
 * Hoechst 33258 pentahydrate bis-benzamide

Ophelia's equipment & molecular biology reagents in Broad

 * Equipment
 * Refrigerated shaker (basement of Broad, Rm 40)
 * Temperature block
 * Gilson pipetmen (P2, 20, 100, 200, 1000)
 * Galaxy ministar centrifuge (for 1.5 ml and PCR tubes)


 * Restriction enzymes
 * AgeI
 * ApaI
 * AscI
 * BamHI & BamHI-HF
 * 2*BbsI (not stable for longer than 30 days at -20C-->according to NEB, check if still functional)
 * BstXI
 * Bsu36I
 * CIP
 * DpnI
 * EcoRI & EcoRI-HF
 * KpnI
 * NotI
 * NruI
 * PacI
 * PspOMI
 * SacI-HF
 * SalI-HF
 * XbaI
 * XhoI


 * PCR enzymes & reagents
 * small amount of dNTPs
 * T4 DNA Ligase
 * Platinum Taq
 * Taq
 * Pfx
 * Small molecules
 * Arabinose
 * aTc
 * Doxycycline
 * IPTG


 * Antibiotics
 * Kanamycin sulfate
 * Ampicillin
 * Chlorampenicol
 * Streptomycin
 * Geneticin (KAN)
 * Hygromycin (HPH)
 * Nourseothricin sulfate (NAT)


 * Kits
 * Clonetech yeast transformation
 * Zymo research E coli competent cell
 * Zymo yeast genomic DNA isolation
 * Qiagen mini, PCR cleanup, gel purification
 * Quikchange kit for mutagenesis


 * Sugars
 * Galactose
 * Glucose
 * Raffinose
 * Sucrose


 * Liquid and plate media materials
 * Bacto Peptone
 * Bacto Yeast Extract
 * Bacto Agar


 * Yeast transformation materials
 * salmon sperm DNA