IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/02

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Thursday 2nd September

 * The overnight digestions were checked using gel electrophoresis [].


 * The gel was set up as follows starting from the bottom:

1) GFP plasmid from the lab. 2) Part BBa_E0040. 3) Ladder.


 * Both bands in lane 2 were removed using the gel extraction kit.[]

* Ligation of pgaA promoter, Part BBa_E0040 and pSB1C3:

pgaA promoter - 2μL Part BBa_E0040 - 2μL pSB1C3 - 2μL T4 DNA ligase - 1μL T4 buffer (x10) - 2μL H2O - 11μL


 * Ligation failed as shown by the bellow gel:




 * The ligation is in lane one. The three bands show that ligations was not successful.


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