User:Karmella Haynes/Notebook/Polycomb project/2010/05/09

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05/09/10

 * &#x2713; Pc-ATF neg ctrl lines: screen for RFP; choose 6 col's per line
 * &#x2713; Misc cell culture: humidifying water was dried up in the incubator; lost 126-4; split others (2x 10cm plates, one for frozen stocks)
 * &#x2713; KAH126-4: thaw new stock (last one!)

Pc-ATF neg ctrl lines RFP screen > 96-well plates, 32 wells per line > Screen for nuclear dox-inducible expression of RFP; RFP+ in dox+ and RFP- in dox- > Key (dox+/dox-) --> - = no expression --> + = weak/ non-uniform expression --> ++ = low or medium uniform expression --> +++ = very strong expression (uniform or non-uniform) --> ? = odd morphology/ strange RFP localization

--> Note: KAH159 clones with ++ expression show a strange single bright dot of RFP, plus medium level RFP expression throughout the nucleus. Nuclear protein aggregate?

--> Tomorrow: transfer dox- cells to 24-well plates


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