User:Hetmann/Notebook/2006-11-7

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Goals for November/December

 * 1) Relabel and organize tubes within BL5088 and BL5096.
 * 2) Starting the week of Monday, 11/13, we are shooting for a goal of one successful ligation per week.  Ligations of all constructs should be complete before Christmas break.
 * 3) *Ligations:
 * 4) *# 'J04500+KaiA+J04500+KaiC'+'J04500+KaiB'
 * 5) *# 'Promoter(rbs)+'KaiA'
 * 6) *# 'B0015'+'J04500+KaiB'
 * 7) *# 'B0015'+'J04500+KaiC'
 * 8) *# 'B0015+J04500+KaiB'+'B0015+J04500+KaiC'
 * 9) *# 'Promoter(rbs)+KaiA'+'B0015+J04500+KaiB+B0015+J04500+KaiC'
 * 10) *Progression
 * 11) ** Step 1: Midiprep
 * 12) **# KaiA
 * 13) **# B0015
 * 14) **# J04500+KaiB
 * 15) **# J04500+KaiC
 * 16) **# Promoter(rbs)
 * 17) **# J04500+KaiA+J04500+KaiC
 * 18) ** Step 2: Digestion
 * 19) **# KaiA/XP
 * 20) **# Promoter(rbs)/SP
 * 21) **# B0015/SP important
 * 22) **# J04500+KaiB/XP important
 * 23) **# J04500+KaiC/XP
 * 24) **# J04500+KaiA+J04500+KaiC/SP
 * 25) ** Step 3: CIP/Gel Purification
 * 26) ** Step 4: Ligation/Transformation
 * 27) **# 'J04500+KaiA+J04500+KaiC'+'J04500+KaiB'
 * 28) **# 'Promoter(rbs)+'KaiA'
 * 29) **# 'B0015'+'J04500+KaiB'
 * 30) **# 'B0015'+'J04500+KaiC'
 * 31) ** Step 5: Midiprep
 * 32) **# Promoter(rbs)+KaiA
 * 33) **# B0015+J04500+KaiB
 * 34) ** Step 6: Digestion
 * 35) **# Promoter(rbs)+KaiA/SP
 * 36) **# B0015+J04500+KaiB/XP
 * 37) ** Step 7: CIP/Gel Purification
 * 38) ** Step 8: Ligation/Transformation
 * 39) **# 'Promoter(rbs)+KaiA'+'B0015+J04500+KaiB'
 * 40) ** Step 9: Midiprep
 * 41) **# Promoter(rbs)+KaiA+B0015+J04500+KaiB
 * 42) **# B0015+J04500+KaiC
 * 43) ** Step 10: Digestion
 * 44) **# Promoter(rbs)+KaiA+B0015+J04500+KaiB/SP
 * 45) **# B0015+J04500+KaiC/XP
 * 46) ** Step 11: CIP/Gel Purification
 * 47) ** Step 12: Ligation/Transformation
 * 48) **# 'Promoter(rbs)+KaiA+B0015+J04500+KaiB'+'B0015+J04500+KaiC'
 * 49) Once J04500+KaiA+J04500+KaiC+J04500+KaiB is complete, borrow a chemostat (or build one? =\).
 * 50) * Test the construct, using Western Blot analysis.