IGEM:MIT/2007/Notebook/2007-7-12

Agenda

 * 1) Run Gel on digested E1010 and diagnostic ladder
 * 2) PCR Purify parts
 * 3) Finish standard assembly of vector + E1010 insert
 * 4) Check sequencing results
 * 5) Make Amp+Kan plates and do a colony screening

Running Gels

 * 85V for 45 Min
 * E1010 digests came out fine
 * Diagnostic Digest (used EcoRI and PstI to cut out insert, evaluated insert length) showed 3 bands.
 * Eventually decided that the three bands were: desired vector, desired insert, F2620/B0034 vector.
 * Will run colony screening using multi-antibiotic plates.

Making Amp+Kan plates

 * If you want to add Amp resistance to Kan plates (or vice versa), add 10µL (Amp diluted 10X, will be different number for adding Kan to Amp) per mL of LB+Kan poured on plate.