Quantitation of DNA

HOW TO ... quantitate DNA, Rutgers, December 2004


 * 1) Equipment needed
 * 2) *UV spectrophotometer
 * 3) *100 ul UV cell
 * 4) *vortex
 * 5) *spin
 * 6) Reagents needed
 * 7) *H2O DNA grade
 * 8) Other consumables
 * 9) *2.5 ml tubes, sterile
 * 10) *gloves
 * 11) *autoclaved/sterile tips
 * 12) Procedure
 * 13) *dilute samples 100 times (2 ul product + 198 ul H2O)
 * 14) *read OD at 260 and 280 nm, reading time should be > 6 sec
 * 15) *use the average of the two readings
 * 16) *OD260 of 1 corresponds to a concentration of 50 ng DNA/ul or to 40 ng RNA/ul
 * 17) *OD260/OD280 should be higher than 1.6, a lower value indicates the presence of proteins