User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/11

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Jurkat qPCR results, Primary antibody WB, Ending stimulation ELISA
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Western blot of yesterday was cut in 3 and incubated with primary antibody
 * AKAP450 (mouse, BD Bioscience, 611518)
 * RIIα(mouse, BD Bioscience, 612243)
 * AKAP95 (mouse, Transduction Laboratories, 610995)
 * GAPDH qPCR
 * As internal control also GAPDH need to be measured,
 * 20 µL reaction volume, as given by the machine
 * Human GAPDH primer (Applied biosystems)
 * Taqman Gene expression master mix

Attachment
   [[Media:10082010_qPCR_AKAP12,_AKAP9.xls|qPCR Jurkat expression AKAP12, AKAP9, GAPDH]]

   [[Media:HTERT_Donor_9_P25_11082010.xls|ELISA VIABILITY STAINING ONLY (Alamar blue)]]


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