User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/27

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=Objectives=
 * Plasmid extraction from yesterday cultures
 * Restriction of Purified PCR.
 * See in a gel extraction.

Plasmid extraction

 * Performed with LB-liquid mediums from yesterday.
 * I used Miguel´s Lab miniprep.
 * Final dilution in 50μL

Restriction

 * In order to standardize pBBRmc-S5 I will cut PCR Purification with SacI & ApaI.


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