User:Wilfred J. Poppinga/Notebook/Wilfreds Project/2009/08/12

 .todo { color: red } .done { color: green} {| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Jolanda's MBP-ArsR fusion protein

 * Transformation of her ligation failed, as to be expected.

Miscellaneous
---
 * 12 μL MBP-Ars
 * 1.5 μL Tango Buffer
 * 0.5 μL XbaI
 * 1 μL PstI
 * total 15 μL
 * 1.5 hour @ 37 °C
 * 80 C
 * 80 C

Comments

 * TOPO® TA Cloning® Kit for subcloning .. ask someone


 * }

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title

 * Insert content here...

Comments

 * [leave a comment!]


 * }