User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/24

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

01/24/10

 * &#x2713; Minipreps: KAH140, 141/V0201 (4 each)
 * &#x2713; PCR colony screen: KAH94, 95/ JDS48, 49 plates

Minipreps > Check with E/P digests (Oops, cut with E/P instead of XbaI) --> E/P cuts 94 bp fragment from backbone vector

PCR colony screen > Use RFP1 primer mix > Controls: (+) = KAH94 (0.5 μL); (-) = JDS48 (0.5 μL)

--> BioRad 96-well: 95°C/ 3 min.; [95°C/ 30 sec., 57°C/ 30 sec., 72°C/ 30 sec.] x30; 72°C/ 3 min. --> All look positive. Tomorrow check streaks for HS4


 * }