User:Karmella Haynes/Notebook/miR Trigger/2010/02/16

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02/16/10

 * &#x2713; RFP knock-down experiment: plate cells for transfection (tomorrow)

RFP knock-down Expt. > Plate cells in 6-well dishes (~5.0x105/well): ---
 * 1) JDS33-47 (HPK-RFPrep-siRNAtarget, 0 Dox)
 * 2) JDS33-47 (HPK-RFPrep-siRNAtarget, 24 hr. Dox)
 * 3) JDS33-47 (HPK-RFPrep-siRNAtarget, 48 hr. Dox)
 * 4) JDS33-47 (HPK-RFPrep ctrl, 0 Dox)
 * 5) JDS33-47 (HPK-RFPrep ctrl, 24 hr. Dox)
 * 6) JDS33-47 (HPK-RFPrep ctrl, 48 hr. Dox)
 * 1) JDS33-47 (untransfected, 0 Dox)
 * 2) JDS33-47 (untransfected, 24 hr. Dox)
 * 3) JDS33-47 (untransfected, 48 hr. Dox)
 * 4) U2OS plain* (HPK-RFPrep-siRNAtarget)
 * 5) U2OS plain* (HPK-RFPrep ctrl)
 * 6) U2OS plain* (untransfected)


 * Use U2OS Flp-in T-REx instead since U2OS cells aren't ready yet (also split 1x 1:10 flask)


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