Registry/Measurement kit/Notebook/2007-7-31

E0240

 * Noticed that during the scarring PCR, a point substitution was made.
 * Decided to try a site-directed mutagenesis on the biobricked product.
 * Ordered two pairs of primers:
 * First pair - mutation in the ends of the primers:
 * CGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGC, E0240_mut_E_F (78.8)
 * CTCTCTTTTCGTTGGGATCTTTCGAAAGGGCAGATTGTGTGGACAGG, E0240_mut_E_R (79.7)
 * Second pair - mutation in the middle of the primers:
 * CCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTG, E0240_mut_M_F (80)
 * ACCATGTGGTCTCTCTTTTCGTTGGGATCTTTCGAAAGGGCAGATTG, E0240_mut_M_R (79.3)

I2057

 * Re-transformed both versions with ccdB into DB3.1

I2055 and I2056

 * Letting biobrick PCR run overnight.

T9002

 * Re-tried, transformed ligation of F2620.

Misc

 * Made chemically competent DB3.1 cells.