Matt Gethers/20.380 HIV Project/Meeting Notes/3.11.09 Presentation Preparation

=3.11.09 Presentation Preparation=

Slides
Matt's Slides

Potential Questions (Some addressed in discussion)

 * 1) MTT Assay for cell viability?
 * 2) Metabolism of analog into potentially harmful molecule (KP-1212 is a metabolite of DHAC, something used as a chemotherapeutic). 5-OH-dC could be metabolized to 5-OH-Uracil or Uracil glycol (not particularly harmful, but modifies nucleotide pool).
 * 3) p24 vs. Viral titer discrepancy?
 * MOI: virus to cell?
 * CEM, CHO, MT-2 cells?
 * 1) Why viral inhibition higher at 10 &mu;l than 100 &mu;l (page 4).
 * 2) Increased sensitivity to drugs when passaged with KP-1212.
 * 3) KP-1461: never mentioned after title?