IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/06

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Monday 6th September

 * Multiple overnight restriction digests were set up as shown bellow:

1) Part BBa_E0040:  Plasmid - 10μL   Pst1 - 2μL   EcoR1 - 2μL   EcoR1 buffer - 2μL   BSA (x20) - 1μL   H2O - 3μL

2) Part BBa_E0040:  Plasmid - 10μL   Xba1 - 2μL   EcoR1 - 2μL   EcoR1 buffer - 2μL   BSA (x20) - 1μL   H2O - 3μL

3) pgaA promoter:  Plasmid - 10μL   Spe1 - 2μL   EcoR1 - 2μL   EcoR1 buffer - 2μL   BSA (x20) - 1μL   H2O - 3μL

4) CsrB promoter:  Plasmid - 10μL   Spe1 - 2μL   EcoR1 - 2μL   EcoR1 buffer - 2μL   BSA (x20) - 1μL   H2O - 3μL

5) HapR promoter:  Plasmid - 10μL   Spe1 - 2μL   EcoR1 - 2μL   EcoR1 buffer - 2μL   BSA (x20) - 1μL   H2O - 3μL

6) Cholera System:  plasmid - 10μL   Hind3 - 2μL   Nco1 - 2μL   Buffer 2 - 2μL   H2O - 4μL


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