User:Karmella Haynes/Notebook/Polycomb project/2010/01/12

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

01/12/10

 * &#x2713; Polycomb-ATF lines: screen for dox-inducible RFP expression
 * &#x2713; Split U2OS Flp-in T-REx: 1x 1:10 flask

Polycomb-ATF lines > Check for dox-inducible RFP --> Each table cell = Dox- / Dox+ --> &#x2713; = lines kept for further testing
 * - = no RFP
 * + = very few RFP+ cells/ very little expression
 * ++ = about 30 - 60% expression
 * +++ = about 60 - 90% expression
 * ++++ = nearly 100% expression and very bright
 * X = dead cells


 * }