Template:SBB10 ConstructionFiles DorothyT sbb25

Construction File of sbb25 PCR sbb25-F and sbb25-R on pEC22-CA42   (661 bp, EcoRI/BamHI) Sub into pBca9523-Bca1455             (EcoRI/BamHI, 2472+915, L) Product is pBca9523-sbb25

sbb25-F  Cloning of sbb25   ccataGAATTCatgAGATCTcagctgaagtgaccggattagc sbb25-R  Cloning of sbb25   ctgatGGATCCgcacgccctttgccggagatgcgaag



JCA Notes

 * Correct