User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/17

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12/17/09

 * &#x2713; Assemblies: re-do KAH117-119, 135, 136

Assemblies
 * 1) KAH117: KAH99/(E/S)/2913 &#x2713; + (1) KAH93/(E/X)/8039
 * 2) KAH118: KAH100/(E/S)/3085 &#x2713; + "
 * 3) KAH119: KAH101/(E/S)/3157 &#x2713; + "
 * 4) KAH135: KAH78/(E/S)/3652 &#x2713; + (2) KAH134/(E/X)/4600
 * 5) KAH136: KAH78/(E/S)/3652 &#x2713; + "

> Digests (Fermentas FD) --> Re-do, do not phosphatase treat; use less DNA to ensure complete digestion

> Measure conc.'s

> Ligations


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