User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/05/05

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 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
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Repeating the luciferase band purification

 * I repeated what I did yesterday (restriction and electrophoresis). This time, I was able to purify a 414.4μg band of ~1600bp, which is consistent with the described 1653bp of | luciferase.


 * After following the protocol, I ended with a 50μl tube with purified luciferase ready for ligation.


 * I ended by doing a restriction assay with ECO R1 and XBA1 of the double terminator | BBa_B0015 (the one augusto had worked in), and left it at 37°C ON.


 * Tomorrow, I will do the ligation.


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