IGEM:IMPERIAL/2007/Notebook/2007-9-6

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7

Testing of Homemade S30 Cell Extract

 * 1) Set up 4 reactions with the following composition
 * 2) *S30 Cell extract 16.2μl
 * 3) *Reaction buffer 30μl
 * 4) *Pyruvate kinase 3.1μl
 * 5) *rNTPs 1μl
 * 6) *DNA/ddH20 4μl
 * 7) *ddH20 5.7μl
 * 8) Left at 37°C incubator
 * 9) *1. Cell extract reaction with pTet-GFP
 * 10) *2. Cell extract reaction without pTet-GFP (negative control)
 * 11) *3. Cell extract reaction with pT7-GFP
 * 12) *4. Cell extract reaction without pT7-GFP (negative control)