IGEM:UNAM-Genomics Mexico/2009/Notebook/HRG personal log/2011/05/21

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title
21/05/2011 06:04 a.m.

I have had little experience in the wet lab. This is the first time I have encountered myself in the laboratory alone.

Before taking the gel out, I had to know know, which was the aim of this gel? (I did not prepare de DNA with which it was loaded and it was not stated in the notebook) I decided to look at the concentration of the gel and based on that decided it was not a gel for extraction. I took the gel out and placed it on the transilluminator

The results were as follows:

Lane 1	 Ladder (1Kb Fermentas)             Ladder

Lane 2	 Forward	                     Negative

Lane 3	 Reverse	                     Negative

Lane 4   No DNA	                     Negative

Lane 5	 No oligos	                     Negative

Lane 6	 5mg DNA	                     Band

Lane 7	 10mg DNA	                     Band

Lane 8 	 Ladder (1Kb Fermentas)	     Ladder

The expectations were those, concordantly, the outcome is a success (we see what was expected). Now the person who did prepare the DNA (Fabricio López) can use this results to the pertinent analysis.


 * }