Registry/Measurement kit/Notebook/2007-7-4

Colony PCR

 * Yet again, yesterday's PCR seems to have evaporated...
 * Trying again with a different tube.

E0240

 * Made glycerol stocks of colonies 3,4,6 and mini-prepping the remaining 4mL.
 * Will send for sequencing today.
 * Sequencing order: 3F, 3R, 4F, 4R, 6F, 6R

I2057

 * Sequence matches!

T9002

 * Starting sequential digest (MfeI, then NsiI).

Primers

 * Need scarring primers for I2056, I2057
 * I2056:
 * F: Same as I2055
 * R: same as I2055


 * I2057:
 * F: CTTAGTAG CAATTG TCACACAGGAAAGTACTAGATGGC (52.1)
 * R: same as I2055