Lysis Methods

100702
 * first complex cell lysate prep'
 * lysed with 2% SDS, 100mM DTT, in 1X PBS (approx pH = 7.4)
 * 2 mL per 15cm dish
 * Split to multiple samples:
 * Added 15% glycerol, then frozen @ -80
 * Frozen @ -80
 * Frozen @ -20
 * 15% glycerol, frozen @ -20