IGEM:MIT/2006/Notebook/2006-8-7

Sequencing

 * 1) Received (6 samples) with some interesting results - hopefully we remedied and will have complete constructs of both ATF1 and BSMT on 8/8 to begin smell testing
 * 2) Sent new sequencing order (42 samples) --- remember that the ATF1s probably have double RBS parts!! and that although we sequenced to check, we did not use them in our 8/7 ligations.

Ligations/Transformations into Top10 cells

 * 1) R0040ES + A/TEP + B0032.J45014.B0015(2)XP
 * 2) R0040ES + A/TEP + B0030.J45014.B0015(4)XP
 * 3) R0040ES + A/TEP + B0030.J45014.B0015(6)XP
 * 4) R0040ES + A/TEP + B0030.BSMT.B0015(13)XP
 * 5) R0040ES + A/TEP + B0030.BSMT.B0015(14)XP
 * 6) R0040ES + A/TEP + B0030.BSMT.B0015(-)XP
 * 7) R0040ES + A/TEP + B0032.BSMT.B0015(A)XP
 * 8) R0040ES + A/TEP + B0032.BSMT.B0015(B)XP
 * 9) osmYSP + B0032.J45014.B0015(2)XP
 * 10) osmYSP + B0030.J45014.B0015(6)XP
 * 11) osmYSP + B0030.BSMT.B0015(13)XP
 * 12) osmYSP + B0030.BSMT.B0015(14)XP
 * 13) osmYSP + B0030.BSMT.B0015(-)XP
 * 14) osmYSP + B0032.BSMT.B0015(A)XP
 * 15) osmYSP + B0032.BSMT.B0015(B)XP

SAGD

 * Tried the pchB, pchA and pchBA PCR again with new pchBrev primer
 * will run gel to check results on 8/8