Timp:stickydishprotocol

Sticky Film (TMPM) Dish Treatment Protocol

 * Materials:
 * 200 Proof Ethanol
 * H2O (MilliQ)
 * Glacial Acetic Acid
 * 3-(trimethoxysilyl) propyl methacrylate, 98%
 * Procedure (To treat approximately 9 dishes):
 * 1) Add 9.5 mL 200 Proof Ethanol to vial
 * 2) Add 500 µL H2O (MilliQ)
 * 3) Remove 50 µL solution - add 50 µL Glacial Acetic Acid
 * 4) Remove 200 µL solution - add 200 µL 3-(trimethoxysilyl) propyl methacrylate
 * 5) Add 1 mL to each dish - filter sterilized through a .22 µm syringe filter
 * 6) Wash twice with MilliQ water.
 * 7) Sterilize with 70% Ethanol, aspirate and let dry/sterilize under UV in TC hood