IGEM:Peking University/2008/Experiment/PKU 0807018 G1 1

PKU_080718_G1_1

 * Enzyme cutting-pADH-lac2(PCR product)


 * System for double ligation


 * System for single ligation


 * 'PCR- pGREG504-pADH-(lac1/lacS/tet)-50ul
 * Using program lac12MQT


 * Gel running and extraction-promoter(PCR product)


 * Enzyme cutting-AID


 * lanes: intact AID(control)|λmarker|Promoter(single)|promoter(double)|AID|AID


 * Enzyme cutting-pADH-lac1/lacS/tet


 * System for double ligation


 * System for single ligation


 * Single Ligation-pGREG504-pAID-lac2


 * Double Ligation-pGREG504-pAID-lac2-AID


 * Control C1


 * Control C2


 * Control C3


 * Control C4


 * Control C5


 * Gel running-testing-pADH-lac1/S/tet
 * Gel running-testing-digestion-lac2/AID