User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/04/16

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * [ubi-RFP]
 * Stain 30' in Totpro 1:500
 * Wash 3x10' or 4x5' in PBS-T
 * Mount immediately.
 * [GFP-myb, RFP-H2Au]
 * Incubate w/ 1:5 dilution of a-Cyclin B (Mouse Antibody) O/N.
 * Crosses
 * Collected two virgin [MH107,hsFLP/FM7Ag ; mRFP, myb+, FRT42D/CyoAg]
 * Will start cross tomorrow


 * }