IGEM:VGEM/2007/Notebook/2007-6-22

AM

 * George's paper presentation
 * Observed multiple pink colonies of RFP-transformed (Z-competent) cells on agar plate.
 * Observed pinkish broth that was left in shaker incubator overnight @ ~36.5 +/- 0.5 degrees C. Unable to excite red fluorescence with standard long wave UV light (which is optimal for GFP excitation).
 * Cryogenic freezing of transformed cells from broth (DH5alpha E. coli)
 * 1 mL of glycerol
 * 1 mL of cell-containing broth
 * Filled cryogenic tube (2 mL total)
 * Vortexed at level 6 for ~ 6-10 secs
 * Making broth from transformed Z-competent cells
 * 5 mL of LB broth
 * Added scraping of cells to broth (use sterilized wire-loop)
 * Vortexed at level 6 for ~6-10 secs
 * Incubated in shaker @ ~36.5 +/- 0.5 degrees C.

PM

 * Detailed research in photosignalling systems and other possible photoreceptors/ photosensors.
 * (Next week's paper presentations will focus on photobiology of microorganisms)