Paulsson:Facs

Where to do it
Systems Biology FACS facility (accessible through SysBio Intranet )
 * talk to Tricia Rogers
 * No bacteria in the (Aria) sorter!

CBR
 * requires PO, looser rules, several sorters

Thoughts
How to bleach prior to fixation?
 * test in plate reader with live cells
 * using scope lamps/lasers?

Post-arrest maturation?
 * on ice
 * cholamphenicol
 * NaN3

Chloride sensitivity (media?)

Biotech and Bioeng v25 no10 p2485

deg-tag vs. photobleach... which ahs better signal strength