IGEM:MIT/2005/Colby Discussion


 * What antigen do we want to attack? Anything.
 * Tricky part: having generic chain to signal the event.
 * To detect surface binding
 * Flow cytometry to detect presence of antigen
 * George Georgiou at UT.
 * Hellinga at Duke.
 * Induction systems - gene. mod. cell that binds to pheromone to gene expression.
 * Modifications made to fuse antibody: no modifications at all. Just fuse DNA to make fusion protein.
 * Usu. use single chain antibody, not entire igg. Just through binding.
 * "Antibody Structure" to see more ideas about domains.
 * Mechanisms:
 * voltage-gated channels
 * ligang-gated channels
 * dimerization
 * 2 antibodies fuse to 2 diff. cell units and having antibody rec different parts to bring subunits close together to bind.
 * feasibility: depends on size of antigen; protein = easy; 15-20 aa. Small molecule would be harder (probably not).
 * Generic system = potentially difficult. Only limits of proteins.
 * cleavage of transcription factor
 * g proteins