User:Karmella Haynes/Notebook/Polycomb project/2010/02/06

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02/06/10

 * &#x2713; Polycomb-ATF lines: passage, expansion, Dox induction

Polycomb ATF lines > For each cell line (8 total) --> Harvest ~1x106 (from 6-well plates) in 2 mL medium --> Expansion: Plate 1 mL in a 1x 10 cm plate (grow up for frozen stocks) --> Passage: Plate 0.5 mL in 2 wells of a 6-well plate (for Dox induction and expression analysis) --> After cells attach, check for background RFP expression (some showed leaky expression in very few cells) --> Add 1 μg/mL Dox to each sample


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