IGEM:IMPERIAL/2007/Calendar/2007-8-20

We are in Week 7  of the iGEM project

Project Calendar
name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7

Modelling

 * Got most equations for infector detector - up on the wiki
 * Most simulations done
 * Some problems, like with the constitutive promoter - will discuss with Captain ID and protocols team to resolve the issues
 * Need to finish forming all the equations and highlight all the issues with the modelling of both ID and CBD by the end of the week

Vessicles

 * Didn't form any vessicles today
 * Got vessicles for sure, but GFP seems to be only around the membrane - possible reason: GFP was not put in the right quantity at the right step
 * Will need to form vessicles again to verify

Protocols

 * Reaction buffer needed for the home-made cell extract to work - ordered all the reagants
 * Home-made cell extract tests put on hold - will carry on testing the DNA constructs in the commmercial one
 * Tests will be done for pTet, PT7, GFP in vitro

Lab team

 * IPTG solution made for the cell extract experiments
 * Cloned a few more bricks, and re-cloned some that didn;t work earlier

Weekly digest

 * Done and send to all the supervisors