IGEM:Peking/2007/Switch-Notebook/2007-9-6

Check our plate
By smearing competent cells directly onto plates without any transformation procedure, we can check whether the plates with Kanamycin is right.

Result: The plates have some thing wrong and we discarded them!

Colony PCR of I7100 ligation product
Fail!

I7100 digestion test
using H buffer, PstI/EcoRI

Fail!

digestion another time of vector and fragments
using still PstI/EcoRI and H buffer