User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/04

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Primer Design for hRLuc8

 * hRLuc8_EcoRI_Fwd
 * 5'-gATCgAATTCTATggCTTCCAAggTgTACgAC-3'
 * 5'-Spacer-EcoRI-Frame Correction-Annealing-3'
 * Tm = 64, 58 (annealing)
 * %GC = 47, 52 (annealing)
 * hRLuc8_XhoI_Rev
 * 5'-gATCCTCgAgCTgCTCgTTCTTCAgCACg-3'
 * 5'-Spacer-XhoI-Annealing-3'
 * Tm = 67, 57 (annealing)
 * %GC = 59, 58 (annealing

Reaction Setup
5.0 μL 10X Buffer 1.0 μL 1:20 dil'n hgLuc plasmid 2.5 μL Primer "hgLuc Rev XhoI" 2.5 μL Primer "hgLuc For EcoRI" 1.0 μL 10mM dNTP mix 0.5 μL Vent Polymerase 37.5 μL dH2O

Thermo Cycle

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