IGEM:PennState/2006/Plate-Reading

=Plate Reading=

Plate Reading
Time: variable

About
This is a kinetic assay for induction by HSL over a 107-magnitude concentration. Each construct is measured in triplicate.

Media/Reagents/Materials
Plates that are needed: 3+X shaker/growing, X fluorescence, where 2X is number of time points desired.
 * M9+0.1%YE+antibiotic plates
 * 210 mL M9+0.1%YE+antibiotic
 * 96 well growing plates
 * 96 well fluorescence plates
 * 96 well clear bottom absorbance plates

Pre

 * 1) Condition parts on M9+0.1%YE+antibiotic minimal media plates
 * 2) Inoculate 3 single colonies from these plates into M9+0.1%YE+antibiotic media overnight
 * 3) Autoclave 96 well shaker/growing plates
 * 4) Dilute cells down to OD600=0.1

Plate Reading

 * 1) Do not put cells on ice!

For GFP, assay at 30ºC ''

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