User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/17

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03/17/10

 * &#x2713; Minipreps: KAH142 (6)
 * &#x2713; Assembly: KAH142 & 96 into V0201

Minipreps > Check with E/S digests

Assemblies
 * 1) KAH142/V0201: (1) KAH142/(N/S)/4571 + V0201/(N/S dp)/5157
 * 2) KAH96/V0201: (2) KAH96/(N/S/)/2825 + "

> Digests (Fermentas FD) --> Use ApaLI to cut V0120 in KAH96 digest (V0120 expected = 1246, 776, 745, 497)

> Measure conc.'s

> Ligations

--> R.T./ ~10 min., Add to 30 μL Z-DH5α


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