IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/28

{| width="800"
 * style="background-color: #444444"|[[Image:Signalling_button.gif|200px|center]]
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Results from transformation of ligation products
- nothing on plate, even on control plate


 * Reasons ?

- cells non competent anymore (try with fresh competent cells)

- not enough DNA (try with 10μL of DNA)

- very short ligation products

Transformation of ligation products (new)

 * Products to ligate : Pupp, Pspac, agrD, RBS S and RBS W

- new fresh competent TOP 10

- 5μL of DNA (1.5μL of PUC9)

- 2h30 in the incubator