IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-6

August 8th
 * Designed Primers
 * Meeting @4
 * Started overnignt ligation of xylRORF into B0034
 * 12:30-6

xylR total 3732929..3734180

Extended for primers 3732860…3734210
TCGTTAAAGG TGCGATTCTG TTGCTGGCAG TATGGATGGA CTCCGCAACC AAACGCCGTT CTTGATTTTG ATAAAAATTT TCTCAAAGCC GGTTACGTAT TACCGGTTTT GAGTTTTTGC ATGATTCAGC AGGAAAAGAA CCatgTTTAC TAAACGTCAC CGCATCACAT TACTGTTCAA TGCCAATAAA GCCTATGACC GGCAGGTAGT AGAAGGCGTA GGGGAATATT TACAGGCGTC ACAATCGGAA TGGGATATTT TCATTGAAGA AGATTTCCGC GCCCGCATTG ATAAAATCAA GGACTGGTTA GGAGATGGCG TCATTGCCGA CTTCGACGAC AAACAGATCG AGCAAGCGCT GGCTGATGTC GACGTCCCCA TTGTTGGGGT TGGCGGCTCG TATCACCTTG CAGAAAGTTA CCCACCCGTT CATTACATTG CCACCGATAA CTATGCGCTG GTTGAAAGCG CATTTTTGCA TTTAAAAGAG AAAGGCGTTA ACCGCTTTGC TTTTTATGGT CTTCCGGAAT CAAGCGGCAA ACGTTGGGCC ACTGAGCGCG AATATGCATT TCGTCAGCTT GTCGCCGAAG AAAAGTATCG CGGAGTGGTT TATCAGGGGT TAGAAACCGC GCCAGAGAAC TGGCAACACG CGCAAAATCG GCTGGCAGAC TGGCTACAAA CGCTACCACC GCAAACCGGG ATTATTGCCG TTACTGACGC CCGAGCGCGG CATATTCTGC AAGTATGTGA ACATCTACAT ATTCCCGTAC CGGAAAAATT ATGCGTGATT GGCATCGATA ACGAAGAACT GACCCGCTAT CTGTCGCGTG TCGCCCTTTC TTCGGTCGCT CAGGGCGCGC GGCAAATGGG CTATCAGGCG GCAAAACTGT TGCATCGATT ATTAGATAAA GAAGAAATGC CGCTACAGCG AATTTTGGTC CCACCAGTTC GCGTCATTGA ACGGCGCTCA ACAGATTATC GCTCGCTGAC CGATCCCGCC GTTATTCAGG CCATGCATTA CATTCGTAAT CACGCCTGTA AAGGGATTAA AGTGGATCAG GTACTGGATG CGGTCGGGAT CTCGCGCTCC AATCTTGAGA AGCGTTTTAA AGAAGAGGTG GGTGAAACCA TCCATGCCAT GATTCATGCC GAGAAGCTGG AGAAAGCGCG CAGTCTGCTG ATTTCAACCA CCTTGTCGAT CAATGAGATA TCGCAAATGT GCGGTTATCC ATCGCTGCAA TATTTCTACT CTGTTTTTAA AAAAGCATAT GACACGACGC CAAAAGAGTA TCGCGATGTA AATAGCGAGG TCATGTTGTA GCGATGATGA GAATTGTCGG CGTCACATCA G

Primers

TOTAL: Front Gaattcgcggccgcttctagag TCGTTAAAGG TGCGATTCTG

End AATTGTCGG CGTCACATCAG tactagtagcggccgctgcag Reverse complement cagcggccgctactag CTGATGTGACGCCGACAATT

Primers for sequencing:

1/3 through xylR ORF
 * Forward: GCCACTGAGCGCGAATATGC Tm=66
 * Reverse: GCATATTCGCGCTCAGTGGC

2/3 through xylR ORF
 * Forward: CACCAGTTCGCGTCATTGAACGG Tm=68
 * Reverse: CCGTTCAATGACGCGAACTGGTG

order # 1131