User:Robwarden/Notebook/GFP-RGD Force Sensor

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Goal
Create a GFP-based sensor to measure the forces involved in cell motility.

Project Outline

 * 1) Express GFPuv with a C-terminal sortase tag.
 * 2) Replace two of GFP's β-barrel loops with fibronectin's RGD and synergy (PPSRN) loops.
 * 3) Coat PEG/PMMA gels with expressed GFP-RGD.
 * 4) Determine a force/fluorescence curve.
 * 5) Measure cellular motility forces in vivo.


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