Post-Processing of Oligo Arrays on Corning Ultra GAPS slides

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Post-processing slides

 * 1) Using a diamond scribe, etch the non-printed side of the array to denote the boundaries of the printed area
 * 2) UV cross-link printed DNA onto glass substrate with 65mJ of energy in a Stratalinker (650 x 100uJ) and transfer arrays on a slide rack
 * 3) Prehybridization in 5x SSC, 0.1 mg/ml BSA, 0.1% SDS at 42°C for 60 Min. (300 ml MQ H2O, 100 ml filtered 20 x SSC, 4 ml 10mg/ml BSA stock solution (filtered), 4 ml 10% SDS, prewarm in 42°C waterbath for 60 min)
 * 4) Rinse in arrays in 0.1 x SSC for 5 min at RT
 * 5) Repeat step 4
 * 6) Transfer arrays to MQ water and incubate at RT for 30 sec.
 * 7) Spin dry arrays in a table to centrifuge at 500 rpm for 5 min.

NOTES:
 * Use arrays the same day that they are postprocessed
 * USE FOR CORNING ULTRA GAPS SLIDES ONLY, DO NOT USE ON POLYLYSINE COATED SURFACES

Martin Buess

DRAFT VERSION 01.17.06