IGEM:MIT/2005/Signal processing

POC
Ray

Function

 * To eliminate noise from the incoming signal (delivered by the sensor group)
 * To deliver a relatively clean and (usually) modular input to the actuator group

Ideas

 * Continuous to discrete (C/D) converter
 * Thresholding based on complementary mRNA competition

Current Status

 * Threshold device:
 * All parts obtained, and are currently undergoing assembly (ready by monday probably)
 * I1030 with B0015 --> will be complete
 * I1031 with B0015 --> needs R0010/11
 * I1032 with B0015 --> needs R0010/11
 * E0840 with R0051 --> will be complete


 * C/D: Still looking for appropriate proteins (shelved)

Device Depiction

 * Thresholding device:
 * Sample graphs here:


 * C/D converter:

Device Parts

 * Threshold: Have been specified and ordered (see picture above)

Experiments

 * Objectives:
 * Ensure TetR::cI fusion works
 * Ensure lambda::GFP fusion works
 * Ensure antisense inihibits cI
 * Strategy:
 * Fix level of IPTG and then vary TetR levels to see the amount of cI needed to express a certain amount of GFP
 * Change IPTG level, then repeat previous step.

Issues/Questions

 * Do cell lines naturally have TetR repressors? or do I need to insert them?
 * Assembly of various devices: One plasmid? different plasmids?

Need Help With
Nothing.. Nada..

Wishlist

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