IGEM:metu/2009/Notebook/wound dressing/2009/09/12

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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12.09.2009
1. Electrophoresis was done. The plasmids which are numerized as 1,2,6,8, BBa, BBb and BBc were load into gel. Plasmids were about 28 microliter so 5 microliter loading dey was put.

2.Double digestion of EGF,KGF,Lard1, Granulysin and Poxygen were done.

For EGF,KGF, Granulysin and Poxygen; water   - 24 microliter Buffer H - 4 microliter DNA     - 10 microliter Enzymes - 1 microliter EcoRI and 1 microliter SpeI

For Lard1; water   - 19 microliter Buffer H - 4 microliter DNA     - 15 microliter Enzymes - 1 microliter XbaI and 1 microliter pstI