Smolke:Protocols/Pfu Buffer

Summary
10x buffer for PCR with Pfu. Makes 10mL.

Ingredients

 * 2 mL 1M Tris-HCl pH 8.8
 * 333 &mu;L 3M KCl
 * 500 &mu;L 20% Triton X-100
 * 1 mL 100x BSA
 * 200 &mu;L 1M MgSO4
 * 5.967 mL H2O

Protocol

 * Mix, vortex.
 * 1) Store at -20&deg;C

Source
Unknown. Ask Kristy.

Contact
Kristy