Moore:Protocols

E. coli Strain Construction
Working With E. coli Transducing Genes with P1 Phage

Cloning and Molecular Biology
Figuring Out How Much Nucleic Acid You Have 'Round-the-Horn Mutagenesis Gene Stitching Making Genomic Libraries

Protein Purification
Getting the Cells open E. coli ClpX E. coli ClpP E. coli SspB

Protein Analysis
Silver Staining SDS PAGE Western Blot Chemiluminescent HRP Detection

Enymatic Assays
Miller/β-Galactosidase Assay

Translation and Ribosomes
E. coli Ribosome Purification Preparing Translation Extracts Poly-U Translation mRNA Translation

RNA Purification and Analysis
Getting RNA From E. coli Primer Extension