User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/22

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03/22/10

 * &#x2713; Cultures: KAH142/V0201 (4)
 * &#x2713; Assembly: KAH143

Assemblies --> DT03 = 27.7 ng/μL --> KAH010 = 25.1 ng/μL
 * 1) KAH143: DT03/(E/S)/1813 &#x2713; + KAH010/(E/X)/~3234 &#x2713;

> Ligations

--> R.T./ ~10 min., Add 50 μL Z-DH5α


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