User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/30

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June 30th, 2010
I restricted plasmids I extracted yesterday in order to ligate, the restrictions were the following ones:

LuxAB --- XbaI, PstI

Plasmid 18 --- XbaI, PstI

Plasmid J61002 --- SpeI, PstI

And I incubated restrictions to 37°C during 4h, then I dephosphated the plasmids

5μL LuxAB

2μL Plasmid J61002 (in the case of plasmid 18 I used 5μL because it was very diluted)

2μL Antarctic Phosphatese Buffer

1μL Antarctic Phosphatase

H2O

Total 20μL.

And I incubate restrictions overnight at 16°C.


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