IGEM:MIT/2006/Notebook/2006-8-25

Things done today:


 * 1) Minipreped, glyceroled, digested, ran on a gel, ligated to R0011 in 1AT3, and transformed rbs.pchba.term structures
 * 2) Performed site-directed mutagenesis on the correct minipreps of the 30.bat2.30.thi3 structures
 * 3) Glyceroled the correct minipreps of the 30.bat2.30.thi3 structures
 * 4) Sequenced rbs.pchba.term structures along with the osmy.inverter.xxx structures
 * 5) Examined some of the sequencing results although many were inconclusive due to bad sequencing
 * 6) Threw away the indole-knockout osmy.inverter.xxx structures since we found that the supposed cells were nasty smelling

Note: In the future, for competent indole knockout cells, use the ones labeled "IKN" in the indole-knockout box.