User:Kalkao/notebook/Freezing Away a Cell Stock


 * 1) Obtain 40% glycerol from lab supply room
 * 2) Obtain overnight suspension from 37*C incubation room
 * 3) *Turn off the rotor to take out test tube cultures
 * 4) *Restart the rotor at a speed between 7 and 8
 * 5) Obtain one cryogenic vial for each stock
 * 6) Add 1mL 40% glycerol and 1 mL culture to each cryogenic vial
 * 7) *Use the flameboy to sterilize the pipette before extracting from glycerol or test tube
 * 8) *Flame the opening of the glycerol bottle before and after each use
 * 9) *Flame the opening of the test tube before and after each use
 * 10) Place the cryogenic vials in a freezer box
 * 11) Label the freezer box and place in -80*C storage