IGEM:Cambridge/2008/PresentationOutline

Voltage (Ellis)

 * KDP
 * K+ ATPase, import pump
 * PCRed from genome
 * OsmY


 * Flame Photometry
 * Data and graphs


 * GluR0
 * Glutamate gated K+ channel
 * From Synechosystis
 * Constitutive Promoter


 * Voltage Output
 * Pictures

Peptide Signaling (Daniel)

 * AGR System
 * Sender & Reciever


 * Compared to Lux
 * More robust, specific
 * Controlled secretion, not membrane diffusion
 * Multichannel signaling (no crosstalk)
 * Requires expression of membrane channels

Bacillus(Daniel)

 * About Bacillus
 * Pretty Pictures
 * Best-characterized Gram Positive
 * Very safe
 * Genome Sequenced
 * Protein Secretion
 * Not compatible with Gram-neg promoters/RBS

Techniques/Tools:

 * Chromosomal Integration
 * Single Copy
 * Cool diagram: single vs. double x-over
 * Detection: amylase assay & PCR
 * InFusion
 * New Vectors
 * Bacillus RBS & Promoters
 * Two Constituitive, Two Inducible
 * Culled from existing vectors
 * Picture of Bacillus expressing GFP with pUpp
 * New GFP
 * Stefan's page

Modeling (Xiao-hu)

 * The agr-peptide signalling system.
 * Assumptions.
 * Results: Step-like behaviour, system highly sensitive to basal rates of expression.
 * Pattern formation.
 * Some simple models.
 * Patterns with agr-quorum sensing systems: competing populations.
 * Further questions: Concise models, spatial pattern generation with a single signalling molecule.