Winning Lab Report Criteria

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Scholars,

Here is a recipe for a winning module 3 report:


 * Microscope documentation
 * Microscope block diagram
 * All optical elements and distances
 * It is not necessary to document mechanical construction details
 * A picture of the microscope is nice


 * Characterization
 * Table of values for 10x, 40x, and 100x objectives, including
 * Theoretical magnification
 * Measured magnificaiton
 * Theoretical resolution
 * Field of view (diagonal)
 * Bright field images
 * Air Force Target, Ronchi Ruling, 4 micron latex spheres
 * 10/40/100x objectives


 * Fluorescence microscopy
 * Image of fluorescence calibration slide (for flat field correction)
 * Optional: flat field corrected image of 1 micron spheres
 * Combined onion image
 * Bright field onion image with flat field corrected, fluorescent image (color) overlayed
 * Bullet point overview of processing algorithm used to produce image
 * PSF
 * PSF image (.190 micron beads) – preferably zoomed in to a few particles
 * Estimated ½ intensity width of beads
 * Was it the same in both directions?


 * Particle tracking
 * Dry beads
 * X-Y plot of particle tracks and differential track
 * Plot of MSD vs. delta t
 * Brownian motion (1 and 2 micron beads in suspension)
 * X-Y plots of particle tracks (particles in different colors)
 * MSD vs. delta time plots with ensemble average
 * Estimated diffusion coefficients
 * Extra credit: estimate Avagadro’s number (see: http://www3.interscience.wiley.com/cgi-bin/abstract/109884431/ and http://www.math.princeton.edu/~mcmillen/molbio/papers/Einstein_diffusion1905.pdf)
 * Transport in an onion cell
 * X-Y plot of particle tracks
 * MSD vs delta t plot
 * Particle velocity: average, deviation
 * Extra credit: estimate the number of myosin motors per granule

--Steve