IGEM:IMPERIAL/Protocols

Protocols

 * Bacterial Culture
 * LB Amp Plate
 * Transformations
 * Competent Cells
 * Bradford Assay
 * 20 uL Digestion
 * 20 uL Ligation
 * 30 uL Ligation
 * Miniprep of Culture
 * 50x TAE Solution
 * Agarose Gel
 * Measuring DNA Concentration
 * Fusion PCR protocol
 * Western Blot Protocol
 * Protocal for aiia PCR
 * 1 kb Plus DNA Ladder
 * Freezing cells (Protocol from OWW: Making a long term stock of bacteria)


 * PCR v. miniprep (flow chart)

Part Testing Protocols

 * T9002/J37016/J37020 Testing Protocol
 * J37015 Testing Protocol
 * J37015-LuxI Testing Protocol PRELIMINARY VERSION
 * I13207 Testing Protocol UNFINISHED (This part is no longer necessary)
 * J37017 Testing Protocol NOT NEEDED
 * J37022 Testing Protocol
 * S01656 Testing Protocol
 * New S0165 testing protocal

Biosensor Testing Protocol

 * Biosensor Testing: Enzyme Activity
 * Biosensor Testing: Calibration & Characterization