Julius B. Lucks/Bibliography/Gottesman-MicrobiolMolBiolRev-68-2004

= Notes on Gottesman-MicrobiolMolBiolRev-68-2004 =


 * review of history of discovery of lambda - what it was used for in the field
 * establishment of repression and prophage insertion are distinct steps in establlishment of lysogeny

Prophage Insertion

 * Cambell mechanism - 1968 review (28) - Fig 1
 * also (24,31)
 * Hershey - cohesive ends making ring (91) - also (207)
 * DNA ligase helps ring closure - Gellert (68)
 * phage-encoded site-specific recombinase (178,179)
 * see also (87,138) for attP-attB recombination rules - site-specificity gives high efficiency and does not require extensive homology
 * (118) - 15 bp region where recombination occurs - see also 200
 * Guerrini (87) - prophage excision not simple chemical reversal of prophage insertion
 * lambbda mutants unable to excise (84)
 * (49) - diff conditions for favoring integration over excision and vice versa
 * integrase more stable than Xis (201)
 * Xis produced from P_L, Int produced from P_Int
 * P_Int activated by CII - CII coordinates Int synthesis and repressor synthesis (35)
 * after induction, both Int and Xis produced from P_L
 * (49,83) - Guarneros and Court - how Int produced from both P_L and P_Int - Fig 2
 * sib - sit of endonucleolytic RNase cleavage - promoter distal to int
 * N - suppresses termination of certain phage transcripts
 * P_L transcripts contain sib because termination suppressed by N - therefore cleaved and destabilizes int message
 * P_L transcript made after induction does not contain sib b.c. of gene permutation after inserts (Fig 1)
 * P_Int transcripts terminate before sib and are more stable (173,174)
 * start site is within xis
 * terminate at T_Int, and do not contain complete sib
 * excision requires Int and Xis
 * in vitro integration/excision - (77,148,149)
 * Int a type I topoisomerase (115)
 * IHF (host integration factor) required for integration (150,151)
 * 2 subunits - ihfA (hid/himA), and ihfB (hip/himD) (114,136,204)
 * architectural role - bends DNA to form Int-attP-IHF intasome (73)
 * effective integration requires negative supercoils (69,139)

= References =


 * 1) Julius B. Lucks/Bibliography