Alm:PCR

Standard PCR reactions
 * ~100 ng template
 * ~100 pmole each primer
 * buffer to 1X
 * polymerase
 * denature 94-95°C
 * anneal 5°C less than lowest primer hyb temp
 * extend 1’/kb to be amplified

PCR Master Mix (2.5X)
 * 62.5 U/ml Taq DNA Polymerase
 * 125 mM KCl
 * 75 mM Tris-HCl, pH 8.3
 * 3.75 mM Mg(OAc)2
 * 500 uM each dNTP

5'-CATTAG can be useful to add to prevent exonuclease activity from degrading recognition sites