Notebook:Tk/2008/04/17

Transformation results

 * seven colonies on the Monday 4/14 plates
 * all on low or medium concentration transformations

cat sequence from pKV104

 * pC194 sequence located at 974-2008 (comp) of genbank V01277
 * corresponds to pKV104 sequences 1975-3010
 * this includes all of cat gene with a portion of both flanking pC194 genes

1 GATAAAGTCC GTATAATTGT GTAAAAGTcc cggggatccA AAAAGGCCAT 51 ATAACAGTCC TTTTACGGTA CAATGTTTTT AACGACAAAA ACATACCCAG 101 GAGGACTTTT ACATGACCCA AGTACATTTT ACACTGAAAA GCGAAGAGAT 151 TCAAAGCATT ATTGAATATT CTGTAAAGGA TGACGTTTCT AAAAATATTT 201 TAACAACGGT ATTTAATCAA CTAATGGAAA ATCAACGAAC AGAATATATT 251 CAAGCAAAAG AATATGAACG AACAGAAAAC CGACAAAGTC AACGAAATGG 301 CTATTATGAG CGCAGCTTTA CGACACGTGT AGGCACGCTA GAATTAAAAG 351 TACCCAGAAC ACGTGATGGC CATTTTTCAC CCACAGTGTT TGAACGTTAT 401 CAACGAAACG AAAAAGCCCT CATGGCTTCA ATGTTGGAAA TGTATGTATC 451 AGGCGTTTCA ACTCGTAAAG TATCAAAAAT TGTGGAAGAA CTTTGTGGTA 501 AATCCGTCTC TAAGTCCTTC GTTTCTAGCT TAACAGAACA GCTAGAACCT 551 ATGGTTAACG AGTGGCAGAA TCGTTTATTA TCAGAAAAAA ATTATCCTTA 601 CTTAATGACC GATGTACTCT ATATAAAAGT ACGAGAAGAA AATCGAGTAC 651 TCTCAAAAAG CTGTCATATA GCGATTGGAA TAACCAAAGA TGGCGACCGT 701 GAAATTATCG GCTTCATGAT TCAAAGTGGC GAAAGCGAAG AGACCTGGAC 751 AACATTTTTT GAATACCTAA AAGAACGCGG TTTACAAGGT ACGGAACTCG 801 TTATTTCTGA TGCGCACAAA GGATTAGTCT CTGCCATTAG AAAATCCTTC 851 ACCAACGTAA GTTGGCAAAG ATGCCAAGTT CACTTCCTAA GAAATATCTT 901 TACCACCATT CCTAAAAAAA ATTCAAAATC TTTCAGAGAA GCTGTTAAAG 951 GAATTTTTAA GTTCACAGAT ATTAACTTAG CGCGTGAGGC TAAAAATCGA 1001 TTGATTCATG ATTATATCGA TCAACCAAAA TATTCAAAAG CTTGCGCATC 1051 ATTGGATGAT GGATTCGAAG ACGCCTTTCA ATATACCGTA CAAGGAAATT 1101 CCCACAATCG ACTAAAGAGT ACCAATCTAA TTGAACGACT GAATCAAGAA 1151 GTACGCAGAA GAGAAAAGAT TATTCGCATC TTCCCCAATC AAACATCAGC 1201 CAATCGCTTA ATTGGAGCCG TTCTTATGGA CCTACATGAT GAATGGATTT 1251 ATTCTTCAAG AAAATACATC AATTTTGATA AGTAGAAATG GTAAAAACAT 1301 TGTATAGCAT TTTACACAGG AGTCTGGACT TGACTCACTT CCTTTATTAT 1351 TTTTCATTTT TTTAAACTAT TTAATACTAA TGTCTTTTAT AATAGCTTTT 1401 CATATATTAT ATAATCAATC TTTATAAGTC CTTTTATAAA TTTCTTTTCT 1451 ACCATTTTCG ATAAATTCCT GTTTAATGGG GATCCGTCGA CGCTCTCCCT 1501 TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG 1551 AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG 1601 TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA 1651 TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA 1701 TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG 1751 TCCGGCGTAG AGGATCAATC TTCATCCATT CCAAGGTAAA TCCCCCTTCG 1801 CCGTTTCTGT TACCATTATA CACCTTTTGA ACCTTAACGT AAACGTTAAG 1851 TTTTAAAAAA CAATAAAAAA GACGAGCAGC ATACAGCACC CGTCTTTCAC 1901 TTTCCTGTTT AAGCTAAACT TCCCGCCACT GACAGAGACT CTTTTTGAAG 1951 GCTTTCAGAA AGCACTCGAT ACGCGATCTG GAGCTGTAAT ATAAAAACCT 2001 TCTTCAACTA ACGGGGCAGG TTAGTGACAT TAGAAAACCG ACTGTAAAAA 2051 GTACAGTCGG CATTATCTCA TATTATAAAA GCCAGTCATT AGGCCTATCT 2101 GACAATTCCT GAATAGAGTT CATAAACAAT CCTGCATGAT AACCATCACA 2151 AACAGAATGA TGTACCTGTA AAGATAGCGG TAAATATATT GAATTACCTT 2201 TATTAATGAA TTTTCCTGCT GTAATAATGG GTAGAAGGTA ATTACTATTA 2251 TTATTGATAT TTAAGTTAAA CCCAGTAAAT GAAGTCCATG GAATAATAGA 2301 AAGAGAAAAA GCATTTTCAG GTATAGGTGT TTTGGGAAAC AATTTCCCCG 2351 AACCATTATA TTTCTCTACA TCAGAAAGGT ATAAATCATA AAACTCTTTG 2401 AAGTCATTCT TTACAGGAGT CCAAATACCA GAGAATGTTT TAGATACACC 2451 ATCAAAAATT GTATAAAGTG GCTCTAACTT ATCCCAATAA CCTAACTCTC 2501 CGTCGCTATT GTAACCAGTT CTAAAAGCTG TATTTGAGTT TATCACCCTT 2551 GTCACTAAGA AAATAAATGC AGGGTAAAAT TTATATCCTT CTTGTTTTAT 2601 GTTTCGGTAT AAAACACTAA TATCAATTTC TGTGGTTATA CTAAAAGTCG 2651 TTTGTTGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT CCAATTGTCT 2701 AAATCAATTT TATTAAAGTT CATTTGATAT GCCTCCTAAA TTTTTATCTA 2751 AAGTGAATTT AGGAGGCTTA CTTGTCTGCT TTCTTCATTA GAATCAATCC 2801 TTTTTTAAAA GTCAATATTA CTGTAACATA AATATATATT TTAAAAATAT 2851 CCCACTTTAT CCAATTTTCG TTTGTTGAAC TAATGGGTGC TTTAGTTGAA 2901 GAATAAAAGA CCACATTAAA AAATGTGGTC TTTTGTGTTT TTTTAAAGGA 2951 TTTGAGCGTA GCGAAAAATC CTTTTCTTTC TTATCTTGAT AATAAGGGTA 3001 ACTATTGCCG ATGATAAGCT GTCAAACATG AGAATTCCCA TTCCTTAATA 3051 AAAGATAATA ATTTTGAACC ATAACCTTTG TCTTGATAGT TAGGTAACAT 3101 TCCTATTGAG TGTAAATAAA ACATTGTTTT TCCATCAGGT CTTAAAAGTG 3151 TATAGCAATA TGCAAATCCT ATAATTTTAT TATTTTCTTT AGCTATAAAC 3201 CCAAATGAAC TGGTATCATT AAGAAATCCT TTTAAATTAT CAATATCAAA 3251 AACCATATTA TCATCAATTA AAACTTCTTC CATAAACTCA GTCAAGTCCA 3301 GACTCCTGTG TAAAATGCTA TACAATGTTT TTACCATTTC TACTTATCAA 3351 AATTGATGTA TTTTCTTGAA GAATAAATCC ATTCATCATG TAGGTCCATA 3401 AGAACGGCTC CAATTAAGCG ATTGGCTGAT GTTTGATTGG GGAAGATGCG 3451 AATAATCTTT TCTCTTCTGC GTACTTCTTG ATTCAGTCGT TCAATTAGAT 3501 TGGTACTCTT TAGTCGATTG TGGGAATTTC CTTGTACGGT ATATTGAAAG 3551 GCGTCTTCGA ATCCATCATC CAATGATGCG CAAGCTTTTG AATATTTTGG 3601 TTGATCGATA TAATCATGAA TCAATCGATT TTTAGCCTCA CGCGCTAAGT 3651 TAATATCTGT GAACTTAAAA ATTCCTTTAA CAGCTTCTCT GAAAGATTTT 3701 GAATTTTTTT TAGGAATGGT GGTAAAGATA TTTCTTAGGA AGTGAACTTG 3751 GCATCTTTGC CAACTTACGT TGGTGAAGGA TTTTCTAATG GCAGAGACTA 3801 ATCCTTTGTG CGCATCAGAA ATAACGAGTT CCGTACCTTG TAAACCGCGT 3851 TCTTTTAGGT ATTCAAAAAA TGTTGTCCAG GTCTCTTCGC TTTCGCCACT 3901 TTGAATCATG AAGCCGATAA TTTCACGGTC GCCATCTTTG GTTATTCCAA 3951 TCGCTATATG ACAGCTTTTT GAGAGTACTC GATTTTCTTC TCGTACTTTT 4001 ATATAGAGTA CATCGGTCAT TAAGTAAGGA TAATTTTTTT CTGATAATAA 4051 ACGATTCTGC CACTCGTTAA CCATAGGTTC TAGCTGTTCT GTTAAGCTAG 4101 AAACGAAGGA CTTAGAGACG GATTTACCAC AAAGTTCTTC CACAATTTTT 4151 GATACTTTAC GAGTTGAAAC GCCTGATACA TACATTTCCA ACATTGAAGC 4201 CATGAGGGCT TTTTCGTTTC GTTGATAACG TTCAAACACT GTGGGTGAAA 4251 AATGGCCATC ACGTGTTCTG GGTACTTTTA ATTCTAGCGT GCCTACACGT 4301 GTCGTAAAGC TGCGCTCATA ATAGCCATTT CGTTGACTTT GTCGGTTTTC 4351 TGTTCGTTCA TATTCTTTTG CTTGAATATA TTCTGTTCGT TGATTTTCCA 4401 TTAGTTGATT AAATACCGTT GTTAAAATAT TTTTAGAAAC GTCATCCTTT 4451 ACAGAATATT CAATAATGCT TTGAATCTCT TCGCTTTTCA GTGTAAAATG 4501 TACTTGGGTC ATGTAAAAGT CCTCCTGGGT ATGTTTTTGT CGTTAAAAAC 4551 ATTGTACCGT AAAAGGACTG TTATATGGCC TTTTTACTTT TACACAATTA 4601 TACGGACTTT ATCTACATCA GAAAGACTTA CGACTCCTGT AATGATAGCA 4651 ACAACCGCTC CAATTAAAGC TGTAATACCA ATATCTAAAC CTTTTGGTTG 4701 CCAAATCACA AAGGTTAAAG TTAAAAGAAA AATTACAATT GCTAAAATAG 4751 TCATCAACAG TCACCTGATT TCACATTTTT ACATACACAT CTTTGATTAG 4801 ATGTATTAAT GATGTTCAAG TTTTGGATAA TATCATCTAA AATAGCATGA 4851 TTAAGTTGAT ACCAATGTTT ATTGCCATCT TTTCGTGTTG TAACTAATTC 4901 ATTATCTACT AATGACTTCA TATGATGACT TAGTGTAGGT TGTGAGAATT 4951 GAAAGTGTTC TAATAAGTCA CAAGCGCATA GCTCACCACA AGAAAGTAAA 5001 TCTAATATTT CTAACCTACT TGGATCTGAT AAAATTTTTA ATATTGTTGA 5051 TAGTTCTTTA TAAGACATGA ATAAACCTCC TAGATATTAA ATAGATTAAC 5101 ATCTATATAG ATTATTGTCT ATGTTATTTT ATTTACTATT TTTTATTCTA 5151 TATATAGTTT GACGTGTAAT ACCAACTTCT TTAGCTATAG TACTTATAGA 5201 TTTACCCTGT TCAAGTAATT CAACAACTCG GTAATAAACT AAACGCTTTT 5251 GTGGGTCTTT AGCATTGGGA GAATACAGAA CAGGTCTCCC TTTATATATA 5301 CCTTTTTCTT TGGCGACTTG AATCCCTTGA GCTTGTCGAC CTCGAGGGGG 5351 GGCCCGGTAC CCAATTCGCC CTATAGTGAG TCGTATTACA ATTCACTGGC 5401 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 5451 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 5501 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG 5551 GAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA 5601 AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA 5651 AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC 5701 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC 5751 CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT 5801 TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC 5851 CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA 5901 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG 5951 TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG 6001 GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 6051 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 6101 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA 6151 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 6201 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 6251 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 6301 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 6351 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 6401 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA 6451 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 6501 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 6551 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 6601 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 6651 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 6701 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA 6751 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 6801 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 6851 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 6901 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 6951 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 7001 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT 7051 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 7101 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 7151 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 7201 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 7251 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 7301 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC 7351 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 7401 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 7451 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 7501 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 7551 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 7601 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG 7651 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 7701 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 7751 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 7801 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 7851 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 7901 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA 7951 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 8001 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG 8051 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG 8101 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 8151 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG 8201 AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCTCTAG GGCTGCAGAA 8251 ACGATATTAA CTAAGTTACT TACAATTAAG GGAAGTGATG TAGTATCAGC 8301 AATAAAACCA CTGGCAATAA TAAAGGGGAA AATCACTTTT TGATTAAATC 8351 CTAGATTCCT TACCATCGCT AATACAATAG GCGTTAAGAT TAAAGCTGCA 8401 CCATCATTTG CGAAAAATGC TGCTACAATT GCCCCAAGTA ACATAATAAA 8451 AACAAACATT TTTAAGCCAT TACCGTTTGA AGCCTTGACC ATATGTATCG 8501 CAGACCATTC AAAAAAACCA ATTTCATCTA ATATTAATGA AATAAGAATA 8551 ACAGCCACAA AAGTTAAAGT TGCATTCCAA ACAATACCTG TTACTTCTAA