Template:SBB10Project-19244

Amy Kristofferson

sbb22: FokI Cleavage Domain (fok-)
Source: Registry part BBa_K243001  in vector BBa_K157000 Target Sequence: CAGCTGGTTaaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaaccagACggtgccatttataccgttggttccccgatcgattatggcgttatcgtggAcacaaaagcgtattctggcggttataatctgccgattggtcaggctgatgagatggaacgttatgtggaagagaatcagacccgtaacaaacatctgaacccgaacgaatggtggaaagtgtatccgtcaagtgtcaccgagttcaaatttctgttcgtgagcggccactttaaaggcaactataaagcccagctgactcgtctgaaccatatcaccaatTgcaatggggcagtgctgagtgttgaggaactgctgatcggtggagaaatgatcaaagcaggcaccctgactctggaagaagttcgccgtaaattcaacaatggcgagatcaatttt Vector: pBjk2741 Short description:  Flavor: { } Family: Restriction Endonuclease Your part exists in the Registry of standard biological parts, but this template sequence differs from what you want by 3 mutations and 3 extra AA at N-term. Take careful note in designing your oligos that you generate the correct product.

sbb36: P9 origin of replication
Source: pBca9145-jtk2642 Vector: pBca9523 Short description: {ColE2 ori med} Family: Replication protein