IGEM:Peking/2007/Switch-Notebook/2007-8-26

experimenter:JLL, LC

colony PCR to check any successful ligation between lacIq and pcc009


one lane seems to be right, be sequenced tomorrow

double promoters colony PCR


there are some loading problems after 10th lane

colony PCR of lacI-pcc009
easy taq 0.1uL pkan-F 0.5uL pcc-test-R 0.5uL buffer 2uL dNTP 2uL ddH2O 15uL

1-9: lacI-pcc009, all wrong 10: self-ligation control 11-13: positive transformation lacI-pcc009

ligation of plx012 with CD2, sulA, RS2 and RD3
fragment 4uL, ligation kit 5uL, plasmid 1uL overnight under 4 degree