User talk:Nkuldell:M13 redesign

M13.1:rough sketch
Note: modified parts codon varied to remove direct repeats.

RBS as identified in Gene (1980) 11:129-148
Renumbered bp according to M13K07 genome annotation 16S RNA 3'OHAUUCCUCCACUAG Note: All RBS are directly followed by ATG of coding seq then a codon starting with A, except gene 6, gene 7 and gene I which follow ATG with G (genes 1 and 7) or C (gene 6). Genes 1, 6 and 7 are translated only to low levels in vivo and in vitro.

Promoters as identified in Gene (1980) 11:129-148
Renumbered bp according to M13K07 genome annotation -10 is TATAATpu centered around -8 from mRNA initiation point -35 is TGTTGACAATT centered around -35 from mRNA initiation point +2 position is often T Many of these promoters were identified as DNA fragments that could bind RNAP

Terminator sequences
Intergenic region I between genes VI and II, contains ori and PS Intergenic region II between genes VIII and III, contains transcription terminator