IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-10

Another mistake when transforming (0：10:10)
The following experiment takes place between 0:30 to 0:50 Qu:Should i centrifuge these tubes ? Zheng:Just do it. Qu: Roger that(centrifuging)... after 5 minutes....(centrifuge finished) Yu:where's the tubes.... Qu:I've just Finished centrifuging ... Yu:these tubes must be standing in ice for 30 minutes before taking them to .... Qu&Ma&Zheng&Yu: .......... after 10 minutes.... Yu:We can use these tubes for Nagetive control! Zheng:Yep. Ma:GO GO GO... 5 minutes later...(centrifuge finished) Yu:I forgot to mark nagetive control tubes Qu&Ma&Zheng&yu:...........what the ****(di~)...

Bacteria cultivation from CGMCC

 * By Xu Anting and Liu Ting
 * K-12 F+: nutrient bouillon agar with 30℃
 * Wait to see if the protocol from CGMCC (China General Microbiological Culture Collection Centre) is OK...

Transformation plasmid from Biobricks

 * 1) Transformation Biobrick PARTS：pSB1A3, pSB3K3, pSB1AK3, R0010, J23066.