IGEM:IMPERIAL/2008/Prototype/Wetlab/parts/Composite

=Composite Parts=

Seen as how the wiki may soon cause errors due to the size of the previous page (and the affect it was having on us), here is the new parts page, specifically for composite parts...

We'll start looking at these soon and I'll get back to people after the weekend

P43-gsiB (done)
Name: Promoter 43 and RBS gsiB for B. subtilis

Code: K143050

Long: Constitutive promoter 43( BBa_K143013 ) coupled to the strong Ribosome Binding Site gsiB( BBa_K143020 ) from B. subtilis.

P43-gsiB can be used in the context of a Ribosomes per second (PoPS) output generator

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of P43 and RBS-gsiB were obtained from papers 1 2 and the sequence synthesised by GeneArt

Source: P43-gsiB was synthesised by GeneArt

P43-spoVG (done)
Name: Promoter 43 and RBS spoVG for B. subtilis

Code: K143051

Long: Constitutive promoter 43( BBa_K143013 ) coupled to the strong Ribosome Binding Site spoVG( BBa_K143021 ) from B. subtilis.

P43-spoVG can be used in the context of a Ribosomes per seconds (PoPS) output generator

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of P43 and RBS-spoVG were obtained from papers 1 2 and the sequence synthesised by GeneArt

Source: P43-spoVG was synthesised by GeneArt

Pveg-gsiB (done)
Name: Promoter Pveg and RBS gsiB for B. subtilis

Code: K143052

Long: Constitutive promoter veg( BBa_K143012 ) coupled to the strong Ribosome Binding Site gsiB( BBa_K143020 ) from B. subtilis.

Pveg-gsiB can be used in the context of a Ribosomes per second (RiPS) output generator

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of Pveg was obtained from the DBTBS 1 and RBS-gsiB were obtained from papers 2 and the sequence synthesised by GeneArt

Source: Pveg-gsiB was synthesised by GeneArt

Pveg-spoVG (Done)
Name: Promoter Pveg and RBS spoVG for B. subtilis

Code: K143053

Long: Constitutive promoter veg( BBa_K143012 ) coupled to the strong Ribosome Binding Site spoVG( BBa_K143021 ) from B. subtilis.

Pveg-spoVG can be used in the context of a Ribosomes per second (RiPS) output generator

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of Pveg was obtained from the DBTBS 1 and RBS-spoVG were obtained from papers 2 and the sequence synthesised by GeneArt

Source: Pveg-spoVG was synthesised by GeneArt

Phyper-spank-gsiB (Done)
Name: Promoter hyper-spank and RBS gsiB for B. subtilis

Code: K143054

Long: Inducible promoter hyper-spank( BBa_K143015 ) coupled to the strong Ribosome Binding Site gsiB( BBa_K143020 ) from B. subtilis.

Phyperspank-gsiB can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.

IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, ( BBa_K143035 ). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of promoter hyperspank was obtained from the B. subtilis integration vector pDR111 and RBS-gsiB were obtained from papers 1 and the sequence synthesised by GeneArt

Source: Phyperspank-gsiB was synthesised by GeneArt

Phyper-spank-spoVG (Done)
Name: Promoter hyper-spank and RBS spoVG for B. subtilis

Code: K143055

Long: Inducible promoter hyper-spank( BBa_K143015 ) coupled to the strong Ribosome Binding Site spoVG( BBa_K143021 ) from B. subtilis.

Phyperspank-spoVG can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.

IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, ( BBa_K143033 ). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of promoter hyperspank was obtained from the B. subtilis integration vector pDR111 and RBS-spoVG were obtained from papers 1 and the sequence synthesised by GeneArt

Source: Phyperspank-spoVG was synthesised by GeneArt

Pxyl-gsiB (Done)
Name: Promoter xyl and RBS gsiB for B. subtilis

Code: K143056

Long: Inducible promoter xyl( BBa_K143014 ) coupled to the strong Ribosome Binding Site gsiB( BBa_K143020 ) from B. subtilis.

Pxyl-gsiB can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.

Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, ( BBa_K143036 ). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of promoter xyl and RBS-gsiB were obtained from papers 1 2 3 and the sequence synthesised by GeneArt

Source: Pxyl-gsiB was synthesised by GeneArt

Pxyl-spoVG (Done)
Name: Promoter xyl and RBS spoVG for B subtilis

Code: K143057

Long:Inducible promoter xyl( BBa_K143014 ) coupled to the strong Ribosome Binding Site spoVG( BBa_K143021 ) from B. subtilis.

Pxyl-spoVG can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.

Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, ( BBa_K143036 ). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of promoter xyl and RBS-spoVG were obtained from papers 1 2 3 and the sequence synthesised by GeneArt

Source: Pxyl-spoVG was synthesised by GeneArt

Pctc-gsiB (Done)
Name Promoter ctc and RBS gsiB for B. subtilis

Code: K143058

Long: Sigma B dependant promoter ctc( BBa_K143010 ) coupled to the strong Ribosome Binding Site gsiB( BBa_K143020 ) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol 1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.

Pctc has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA ( BBa_K143037 ) is required.

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of promoter ctc was obtained from the B. subtilis genome and published papers 1 2. RBS-gsiB were obtained from papers 3 and the sequence synthesised by GeneArt

Source: Pctc-gsiB was synthesised by GeneArt

Pctc-spoVG (Done)
Name: Promoter ctc and RBS spoVG for B. subtilis

Code: K143059

Long: Sigma B dependant promoter ctc( BBa_K143010 ) coupled to the strong Ribosome Binding Site spoVG( BBa_K143021 ) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol 1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.

Pctc has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA ( BBa_K143037 ) is required.

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of promoter ctc was obtained from the B. subtilis genome and published papers 1 2. RBS-spoVG were obtained from papers 3 and the sequence synthesised by GeneArt

Source: Pctc-spoVG was synthesised by GeneArt

PgsiB-gsiB (Done)
Name: Promoter gsiB and RBS gsiB for B. subtilis

Code: K143060

Long: Sigma B dependant promoter gsiB( BBa_K143011 ) coupled to the strong Ribosome Binding Site gsiB( BBa_K143020 ) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol 1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.

PgsiB has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA ( BBa_K143037 ) is required.

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of promoter gsiB was obtained from the B. subtilis genome and published papers 1 2. RBS-gsiB were obtained from papers 3 and the sequence synthesised by GeneArt

Source: PgsiB-gsiB was synthesised by GeneArt

PgsiB-spoVG (Done)
Name: Promoter gsiB and RBS spoVG for B. subtilis

Code: K143061

Long: Sigma B dependant promoter gsiB( BBa_K143011 ) coupled to the strong Ribosome Binding Site spoVG( BBa_K143021 ) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol 1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.

PgsiB has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA ( BBa_K143037 ) is required.

'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix 1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''

Design: The sequence of promoter gsiB was obtained from the B. subtilis genome and published papers 1 2. RBS-spoVG were obtained from papers 3 and the sequence synthesised by GeneArt

Source: Pctc-spoVG was synthesised by GeneArt

LacI-Terminator (Done)
Name: LacI repressor protein - Terminator

Code: K143062

Long: LacI transcriptional repressor protein ( BBa_K143033 ) coupled to the double terminator ( BBa_B0015.

The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis.

LacI can be used in conjunction with the lac operon promoter ( BBa_K143015 ), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.

Design: LacI was identified from the pDR111 B. subtilis integration vector. The double terminator is the most commonly used registry terminator.

Source: LacI was produced by PCR cloning using Pfu form the B. subtilis integration vector and cloned into a BioBrick with the registry double terminator

XylR-Terminator (Done)
Name: Xylose operon repressor protein - Terminator

Code: K143063

Long: Xylose operon repressor protein(XylR)( BBa_K143036 ) coupled to the double terminator ( BBa_B0015.

XylR can be used in conjunction with the xylose operon promoter ( BBa_K143014 ), where the XylR will act as a receiver for a xylose input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the XylR to be easily incorporated into a closed transcriptional unit.

Design: XylR was identified from the B. subtilis chromosome. The double terminator is the most commonly used registry terminator.

Source: XylR was produced by PCR cloning using Pfu form the B. subtilis chromosome and cloned into a BioBrick with the registry double terminator

YtvA-Terminator (Done)
Name: YtvA light receptor Protein - Terminator

Code: K143066

Long: YtvA light receptor protein (YtvA)( BBa_K143037 ) coupled to the double terminator ( BBa_B0015 ).

YtvA can be used in conjunction with the sigma factor B specific promoter ( BBa_K143010 or BBa_K143011 ), where the YtvA will act as a receiver for a blue light input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the YtvA to be easily incorporated into a closed transcriptional unit.

Design: YtvA was identified from the B. subtilis chromosome. The double terminator is the most commonly used registry terminator.

Source: YtvA was synthesised by GeneArt and cloned into a BioBrick with the registry double terminator

Chloraphemicol Resistance Protein - Terminator (Done)
Name: Chloraphemicol resistance protein - Terminator

Code: K143064

Long: Chloraphemicol acetyltransferase protein( BBa_J31005 ) coupled to the double terminator ( BBa_B0015 ).

Chloraphemicol acetyltransferase confers resistance to Chloraphemicol

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the CAT to be incorporated into a closed transcriptional unit.

Design: Chloraphemicol acetyltransferase is an exisiting registry protein. The double terminator is the most commonly used registry termiantor

Source: The Chloraphemicol acetyltransferase and double terminator were taken both taken from the registry.

Spectinomycin Resistance Protein (Aad9) - Terminator (Done)
Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143065

Long: Aad9 spectinomycin resistance protein( BBa_ ) coupled to the double terminator ( BBa_B0015 ).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

AmyE integratable PoPS generator (P43-gsiB) (Partially Uploaded)
Name: AmyE integratable PoPS generator (P43-gsiB)

Code: K143067

Long: AmyE 5' Integration sequence( BBa_K143001 ) coupled to the PoPS generator P43-gsiB ( BBa_K143050 ).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence( BBa_K143002 ) is cloned onto the 3' end of the construct.

The P43-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-gsiB was obtained from papers.

Source: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-gsiB that was synthesised by GeneArt

AmyE integratable PoPS generator (P43-spoVG) (Partially Uploaded)
Name: AmyE integratable PoPS generator (P43-spoVG)

Code: K143068

Long: AmyE 5' Integration sequence( BBa_K143001 ) coupled to the PoPS generator P43-spoVG ( BBa_K143051 ).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence( BBa_K143002 ) is cloned onto the 3' end of the construct.

The P43-spoVG promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-spoVG was obtained from papers.

Source: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-spoVG that was synthesised by GeneArt

AmyE integratable PoPS generator (Pveg-gsiB) (Partially Uploaded)
Name: AmyE integratable PoPS generator (Pveg-gsiB)

Code: K143069

Long: AmyE 5' Integration sequence( BBa_K143001 ) coupled to the PoPS generator Pveg-gsiB ( BBa_K143052 ).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence( BBa_K143002 ) is cloned onto the 3' end of the construct.

The Pveg-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-gsiB was obtained from papers.

Source: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-gsiB that was synthesised by GeneArt

AmyE integratable PoPS generator (Pveg-spoVG) (Ready for Upload)
Name: AmyE integratable PoPS generator (Pveg-spoVG)

Code: K143070

Long: AmyE 5' Integration sequence( BBa_K143001 ) coupled to the PoPS generator Pveg-spoVG ( BBa_K143053 ).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence( BBa_K143002 ) is cloned onto the 3' end of the construct.

The Pveg-spoVG promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-spoVG was obtained from papers.

Source: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-spoVG that was synthesised by GeneArt

AmyE integratable PoPS generator (P43-gsiB) (with CmR) (Ready for Upload)
Name: AmyE integratable PoPS generator (P43-gsiB) (with CmR)

Code: K143071

Long: AmyE 5' Integration sequence( BBa_K143001 ) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts BBa_K143050 and BBa_K143064 ) and the PoPS generator P43-gsiB ( BBa_K143050 ).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence( BBa_K143002 ) is cloned onto the 3' end of the construct.

The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.

The P43-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-gsiB was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.

Source: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-gsiB that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.

AmyE integratable PoPS generator (P43-spoVG) (with CmR) (Ready for Upload)
Name: AmyE integratable PoPS generator (P43-spoVG) (with CmR)

Code: K143072

Long: AmyE 5' Integration sequence( BBa_K143001 ) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts BBa_K143051 and BBa_K143064 ) and the PoPS generator P43-spoVG ( BBa_K143051 ).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence( BBa_K143002 ) is cloned onto the 3' end of the construct.

The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.

The P43-spoVG promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-spoVG was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.

Source: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-spoVG that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.

AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) (Ready for Upload)
Name: AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)

Code: K143073

Long: AmyE 5' Integration sequence( BBa_K143001 ) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts BBa_K143052 and BBa_K143064 ) and the PoPS generator Pveg-gsiB ( BBa_K143052 ).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence( BBa_K143002 ) is cloned onto the 3' end of the construct.

The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.

The Pveg-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-gsiB was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.

Source: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-gsiB that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.

AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) (Ready for Upload)
Name: AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)

Code: K143074

Long: AmyE 5' Integration sequence( BBa_K143001 ) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts BBa_K143053 and BBa_K143064 ) and the PoPS generator Pveg-spoVG ( BBa_K143053 ).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence( BBa_K143002 ) is cloned onto the 3' end of the construct.

The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.

The Pveg-spoVG promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-spoVG was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.

Source: The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-spoVG that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.

B. subtilis AmyE locus GFP promoter and RBS tester (Ready for Upload)
Name: B. subtilis AmyE locus GFP output promoter and RBS tester

Code: K143075

Long: AmyE 3' Integration sequence( BBa_K143002 ) coupled to GFP proein coding region and the registry double terminator (part BBa_I13401.

The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence( BBa_K143001 ) is cloned onto the 5' end of the construct.

The GFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.

Design: The amyE 3' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. GFP and the double terminator were obtained from the registry.

Source: The amyE 3' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the GFP and the double terminator that were obtained from the registry.

B. subtilis AmyE locus RFP promoter and RBS tester (Ready for Upload)
Name: B. subtilis AmyE locus RFP output promoter and RBS tester

Code: K143076

Long: Long:  AmyE 3' Integration sequence( BBa_K143002 ) coupled to RFP proein coding region and the registry double terminator (part BBa_J04650.

The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence( BBa_K143001 ) is cloned onto the 5' end of the construct.

The RFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.

Design: The amyE 3' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. RFP and the double terminator were obtained from the registry.

Source: The amyE 3' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the RFP and the double terminator that were obtained from the registry.

P43-gsiB GFP expression construct (Ready for Upload)
Name: P43-gsiB GFP expression construct

Code: K143077

Long: GFP gene under expresson of the P43 promoter and gsiB RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.

Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.

This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and gsiB RBS (Combined part BBa_K143050 ) as part of the Biofabricator subtilis project.

Design: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, GFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS gsiB were taken from papers.

Source: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-gsiB part that was synthesised by GeneArt.

P43-spoVG GFP expression construct (Ready for Upload)
Name: P43-spoVG GFP expression construct

Code: K143078

Long: GFP gene under expresson of the P43 promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.

Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.

This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and spoVG RBS (Combined part BBa_K143051 ) as part of the Biofabricator subtilis project.

Design: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, GFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS spoVG were taken from papers.

Source: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-spoVG part that was synthesised by GeneArt.

Pveg-spoVG GFP expression construct (Ready for Upload)
Name: Pveg-spoVG GFP expression construct

Code: K143079

Long: GFP gene under expresson of the Pveg promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.

Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.

This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part BBa_K143053 ) as part of the Biofabricator subtilis project.

Design: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, GFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter Pveg and RBS spoVG were taken from papers.

Source: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the Pveg-spoVG part that was synthesised by GeneArt.

P43-gsiB RFP expression construct (Ready for Upload)
Name: P43-gsiB RFP expression construct

Code: K143080

Long: RFP gene under expresson of the P43 promoter and gsiB RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.

Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.

This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and gsiB RBS (Combined part BBa_K143050 ) as part of the Biofabricator subtilis project.

Design: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, RFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS gsiB were taken from papers.

Source: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-gsiB part that was synthesised by GeneArt.

P43-spoVG RFP expression construct (Ready for Upload)
Name: P43-spoVG RFP expression construct

Code: K143081

Long: RFP gene under expresson of the P43 promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.

Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.

This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and spoVG RBS (Combined part BBa_K143051 ) as part of the Biofabricator subtilis project.

Design: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, RFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS spoVG were taken from papers.

Source: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-spoVG part that was synthesised by GeneArt.

Pveg-spoVG RFP expression construct (Ready for Upload)
Name: Pveg-spoVG RFP expression construct

Code: K143082

Long: RFP gene under expresson of the Pveg promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.

Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.

This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part BBa_K143053 ) as part of the Biofabricator subtilis project.

Design: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, RFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter Pveg and RBS spoVG were taken from papers.

Source: The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the Pveg-spoVG part that was synthesised by GeneArt.

Codes and associated parts
{| border="2" cellpadding="2" style="text-align:left; background:black" ! Code ! Part ! Code ! Part ! Code ! Part ! Code ! Part ! Code ! Part
 * - style=background:springgreen
 * - style=background:powderblue
 * K143050 || P43-gsiB || K143051 || P43-spoVG || K143052 || Pveg-gsiB  || K143053 || Pveg-spoVG || K143054 || Phyperspank-gsiB
 * - style=background:powderblue
 * K143055 || Phyper-spank-spoVG || K143056 || Pxyl-gsiB || K143057 || Pxyl-spoVG || K143058 || Pctc-gsiB || K143059 || Pctc-spoVG
 * - style=background:powderblue
 * K143060 || PgsiB-gsiB || K143061 || PgsiB-spoVG || K143062 || LacI - Terminator || K143063 || XylR - Terminator || K143064 || CAT - Terminator
 * - style=background:powderblue
 * K143065 || Aad9 - Terminator || K143066 || Ytva - Terminator || K143067 || 5' 9 || K143068 || 5' 10 || K143069 || 5' 11
 * - style=background:powderblue
 * K143070 || 5' 12 || K143071 || RI Brick 9 tail || K143072 || RI Brick 10 tail || K143073 || RI Brick 11 tail || K143074 || RI Brick 12 tail
 * - style=background:powderblue
 * K143075 || GFP - Terminator 3' || K143076 || RFP - Terminator 3' || K143077 || P43-gsiB GFP expression construct || K143078 || P43-spoVG GFP expression construct || K143079 || Pveg-spoVG GFP expression construct
 * - style=background:powderblue
 * K143080 || P43-gsiB RFP expression construct || K143081 || P43-spoVG RFP expression construct || K143082 || Pveg-spoVG RFP expression construct || K143083 || || K143084 ||
 * - style=background:powderblue
 * K143085 || || K143086 || || K143087 ||  || K143088 || || K143089 ||
 * -}

Note


 * Aad9 is the Spectinomycin resistance gene
 * RI - Resistance Integration Brick, P - Promoter, Pi - chemically inducible promoter, Pl - light inducible promoter, Bs - B.subtilis, PTC - Promoter Testing Construct, Rep - Repressor protein