Jessica Karen Wong/Notebook/2007-7-4


 * Took out overnight plates of I2056 transformation and saw no more colonies
 * PCR of I2055 also evaporated, redoing with 20ul and different tube
 * Got I2057 sequence which matches registry


 * Preped colonies 3, 4, and 6 of I2055 for sequencing
 * Minipreped and eluted with 30 ul pH 8.5 water instead of EB
 * used the 8-tubes, 2 samples of each colony, VR and VF
 * Mix: 2ul primer, x ul DNA to make b/t 200 and 500 ng, (10-x)ul water
 * Submitted order on dnalims.mit.edu
 * Building was locked so it's in the 4 degree


 * Digested T9002 with Mfe1
 * used buffer 4 and 11 ul T9002


 * Need scarring primers for I2056, I2057
 * I2056:
 * F: Same as I2055
 * R: same as I2055


 * I2057:
 * F: CTTAGTAG CAATTG TCACACAGGAAAGTACTAGATGGC (52.1)
 * R: same as I2055