User:Howard Boland/Notebook/Art from Synthetic Biology/2010/11/10

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Result of overnight growth
The result of the overnight growth was positive but took time to manifest due to someone having changed the temperature in the shaker.

Glycerol Stock
I took glycerol stock of the colony before proceeding with the miniprep
 * 1) 700µl stock
 * 2) 300µl 50% glycerol

store at -80ºC

Miniprep
I then proceeded by minipreping the colony using the QIAgen kit to obtain 30µl plasmid.


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