User:Karmella Haynes/Notebook/BioBrick cloning/2010/06/03

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06/03/10

 * &#x2713; Assemblies: redo MV8 and MV3

Assemblies
 * 1) MV8: MV1/(BsaBI/BstBI)/4605 + Hygro PCR/(BsaBI/BstBI)/1023
 * 2) MV8: MV1/(BsaBI/BstBI)/3919 + "

> Ligations

--> R.T./ 1 hour; Use 25 μL Turbo DH5α; Heat shock and recover for 30 min.

--> Cloning failed. Try Hygro primers with longer extensions to improve cutting.


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