Chang Lab:Notebook/CBE/08/149/2008/12/17

{| width="800"
 * style="background-color: #FFF"|   Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page
 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page


 * colspan="2"|
 * colspan="2"|

Logs

 * Start: 8:30 AM
 * Cultured bacteria (for biofilm optimization trial III next day)
 * Single loop taken from streaked petri dishes on 12/13 and suspended into 10ml broth in a test tube (incubate at 37 C for 24hrs).

Notes on serial plating

 * Plates incubated on 12/15: Sonicated biofilms from Trial I
 * No growth at 10^-6, 10^-8, 10^-10 dilutions ==> REDUCE dilutions for Trial II plating
 * Plates incubated on 12/16: Biofilm Inoculum (matched at 1.0 McFarland)
 * LB: Too many to count at 10^-10, 10^-12, 10^-14 ==> INCREASE dilutions for Trial III plating
 * M9: No growth at 10^-10, 10^-12, 10^-14 ==> Need to rethink centrifuge procedure!!!


 * }