IGEM:IMPERIAL/2008/Prototype/Drylab//Motility data collection/Methods

=Manual Tracking Methodology=


 * We intend to track for each cell:
 * The centroid
 * We attempt to reduce the bias in our manual tracking
 * By disabling the view path option
 * By displacing the cursor away from the tracked cell before adding a new point

Tracking Criteria
Our criteria for choosing cells to track are as follows:
 * 1) Only track cells which exhibit swimming motility. These motile cells must travel in the direction of their longitudinal axis.
 * 2) Cells must be encircled by a high light intensity (white) membrane and a low light intensity (dark) cell body.
 * 3) Single cells or up to 2 single cells attached to each other may be tracked.
 * 4) Once cell moves out of focus or out of the field of vision tracking must end.

The following images show cells which failed the above criteria and hence were not tracked: