User:Wilfred J. Poppinga/Notebook/Wilfreds Project/2009/08/24

 .todo { color: red } .done { color: green} {| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title

 * Safety
 * Engineer
 * Watercampus

Miscellaneous

 * Nanodrop MBP-ArsR
 * 45.9 ng/μL
 * 260/280: 1.88
 * 260/230: 2.05
 * 260/230: 2.05

PCR
21 μL mastermix* 1 μL forward primer 1 μL reverse primer 1 μL template 1 μL Taq polymerase

100 μL Taq NH4 8 μL dNTP's  80 μL MgCl 652 μL MilliQ water  PCR Reaction
 * master mix contains:
 * Hotstart
 * 95 °C, 2 min.
 * 25 cycles
 * 95 °C, 30 sec.
 * 61 °C, 20 sec.
 * 72 °C, 1.5 min.
 * End
 * 72 °C, 10 min.
 * 4 °C, ∞

Comments

 * User:Wilfred_J._Poppinga/Notebook/Wilfreds_Project/2009/08/21


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{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title

 * Insert content here...

Comments

 * [leave a comment!]


 * }