User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/06

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=Wet Lab: OmpC/F promoter=

Electrophoresis Gel OmpF PCR_RTTH
In order to probe the PCRs reactions an electroforesis gel was made. This was loadded as follows:
 * Lane1. Ladder
 * Lane2. PCR1 (pSB3K3 + GFP_OmpF)
 * Lane3. PCR2 (pSB3K3 + GFP_control_bluepromoter)
 * Lane4. PCR3 (pSB3K3 + GFP_control)
 * Lane5. PCR4 (pSB1C3_OmpC)
 * Lane6. PCR5 (pSB1C3_control)



It is had PCR product from the pSB1C3 backbone. The next step is to run a second PCR reaction with the other OmpF REV primer, using as template the product from the PCR4.

Note
[pSB1C3]


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