Template:SBB10Project-37738

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sbb30: O99 rep Gene
Source: pEC52 Target Sequence: aaaaacgattctgacgcattttttatgtttaccctggtgtttccttgtctttttgttttttttgcgtcagaaagcgactgagggtgttttaaggggtacgtacaacgggagttatggtaaatggatcgggttttcgggaaggttcgacaggatttgccgttgggtgtagtgtaagcgactgaaaaacaaacgcccctgaaattcgtgtctctccgccaagattgtcacgagaatacaggggcgaggtcggtcgataacctggaaggaatcgtaacacatgagcgccgcgcttcaatacttcgaagaaaatttaccccaccgtccctatcacacggatgatctcgcttttggtcttcgcatctccggcaaagggcgtgcgcttcttgcggggtacatccagcagaaccagcctcatgcgcagttctggctggtttttgacgttgaccgcgagggggctgcgattgactggagcgaccggaacgcccccgcgccgaacatcaccgtaaaaaatcccgtgaacggtcatgctcacctgctctatgcactcaacatcgccgtgagaaccgcgcctgatgcgtcggttaaggcactgaagtacgctgccgcgatagagcgtgcgctgtgtgagaaacttggtgcagatgtgaactacagcggactgatttgcaaaaatccgttccacctggaatggctggtgatggagtggcgcgaggaagcctataccctcgatgaactggctgattatctcgatttgagcgcctcagcgcgccgtagcatcgataaacattacgggatggggcgaaactgccacctgttcgaaatgacgcgcaaatgggcttacagggcgattcgtcagggctggcctgtattctcacaatggcttgatgccgtgatccagcgtgtcgaaatgtacaacgtatcgcttcccgttccgctttctccggcggaatgtcgggctattggcaagagcattgcgaaatacacgcacaggaacttcacgccggaaactttcgcacagtatgtggctgatacgcacacgccagaaattcaggctgcacgtggtcgcaagggcgggaaaattggaggtgctaagtctaagcgtggtgctgttgccacatctgcgcgaacgttgaaaccgtgggagactcttggaattagccgcgcctggtattaccaactgaaaaaacgaggtcttgtagagtag Vector: pBca9523 Short description: 5'UTR_repO99! Genbank reference: D30060.1 Family: Expressed CDS

sbb38: Pbad promoter
Source: pBjh1601CK-ig114 Vector: pBjk2741 Short description: {AraC-Pbad} Family: Conditional Promoter This part encodes the Pbad promoter and its regulatory protein, AraC. In the presence of the small molecule monosaccharide arabinose, Pbad generates RNA transcripts of downstream DNAs.

sbb37: Constitutive promoter
Source: pBca1100-Bca1152 Vector: pBjk2741 Short description: {Pcon-J23100} Family: Constitutive Promoter This part encodes the a constitutive promoter. It generates RNA transcripts of downstream DNAs "constitutively", or in other words, all the time regardless of whether you add some inducer chemical or not.