IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/03/16

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LovTAP

 * We have a trouble with LovTAP system. Our system to repress and repressor is not 100% effective, because always is an expression independent of light reception.

General

 * Efficiency of Quantum Yield could be possible in our system.
 * There is an optional green light receptor and transcriptional response by same system.

We need to know

 * Is there one way to work with LovTAP system?
 * Best CDE genes of operon Lux.
 * Luciferin-luciferase. How to be produced in E. Coli?
 * How to anchor XFP on a Luciferase?
 * What are the details of green light receptor?