20.109(F08): The grafting parlour

NOTES FROM 11.11 meeting with the artist from THE GRAFTING PARLOUR
Please edit and add information that I didn't capture Nkuldell 16:39, 11 November 2008 (EST)

Initial themes

 * Form brings questions about content
 * Computational approaches represent nature but biology holds in itself the reality of nature
 * Art can tilt and sway perspective
 * Interactive technology (video, telecommunication) are time base media

Framing questions

 * How to look at the world through nature’s point of view?
 * How can artwork change itself during a show?
 * How can artwork change as it travels from gallery to gallery?
 * We value the history of an object but can an object have traces/memories of itself and its history?
 * Is the human desire to “fix time” immutable?
 * We think of our cells as making up us but they have a life of their own as well (circadian pulsing of neurons every 23.5 hours w/o stimulus). What is the biological memory that cells have of a day? What do cells have to say to us?
 * Galleries usually carefully manage light/moisture/temperature to inhibit bacteria growth on art but is this just a romantic notion of art by masters and is the intervention needed? Can unpredictable evolution/passage/change of art be part of art?

Art/Science examples we considered

 * GFP projects from Marc Zimmer: Static images that were not intended as art
 * pt1
 * Brainbow
 * GFPixle
 * Eduardo Kac
 * Transgenetic Alba bunny: use of animals as art? Research was done for science then made accessible through art
 * Specimen of Secrecy About Marvelous Discoveries, pt 1
 * Specimen of Secrecy About Marvelous Discoveries, pt2


 * Prof. Eshel Ben-Jacob
 * Prof. images pt 1
 * pdf


 * Prof. Micha Spira


 * Hyunkoo Lee: animatus= skeletons from animated characters. Notable for its performance of science, that the artist makes apparent. Some eerie some playful examples.

NOTES: about microscope/display
from Lucy 12.08.08 one that works with existing analog microscopes: at: =G1 to S transition=
 * There are ways to convert a webcam into a microscope but my guess is that the magnification is not great.
 * Whether microscope or document camera, it depends on how wide a view will be captured. Here is
 * And here is more information on the document camera that I've been looking

Primers to check Cln1:GFP and Cln2:GFP strains
From intro of Fred Cross paper on Clb2: "Cyclins and cyclin-dependent kinase (Cdk) provide excellent candidates for a central controlling timer. In all eukaryotes, mitotic cyclin–Cdk activity rises and falls once per division. Mitotic cyclins (CLB1, 2, 3, and 4 in S. cerevisiae) are required for mitotic entry (spindle assembly and anaphase). However, overexpression of mitotic cyclin prevents mitotic exit (spindle disassembly and cytokinesis), resulting in telophase arrest (Surana et al, 1993 8491189)." So perhaps a system in which CLN1:GFP fusion is controlled on a plasmid and is Gal inducible might arrest at S?

Cln1= 1641bp
NO270=Forward: CCCT TTTCTCTCTATGCCCAT NO271=Reverse: CTAG ATGTTTGTAGGTGGGCA PCR product = 977bp PCR product+GFP = 1690bp
 * length = 21
 * Tm = 54.3
 * GC = 47.6
 * length = 21
 * Tm = 54.3
 * GC = 47.6

Cln2 = 1638 bp
NO272= Forward: CACGGCATATTCTCCATTATC NO273= Reverse: GGTCTCTTTTTGGTACGTTTG PCR product = 804bp PCR product+GFP = 1517bp
 * length: 21
 * Tm = 50.9
 * GC = 42.9
 * length = 21
 * Tm = 51.8
 * GC = 42.9

Additional Primers
=G2 to M transition=

Clb2= 1475bp

 * Reference with Clb2:YFP is here. This reference also includes (as Fig 1) depiction of CFP-TUB1 to label cytoskeleton blue, as well as MYO1-mCherry to label red the contractile ring from cytokinesis.