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Nikhil Sharma

TraT
Source: pOX38 You will be making a C-terminal display system out of the TraT protein from F plasmid. pOX38 is just a particular variant of the F plasmid. You should read PMID: 9933744 and PMID: 10725535 for background. Unlike in these reference, you will remove the prepro region of the protein in your part. You should make the same C-terminal sequence present in the references as the last amino acids before the BamHI site. You will not be including any of the thrombin site/other modifications that were done in these references.

The sequence of TraT is available at NC_002483 in pubmed.

You can use the online tool at http://phobius.cbr.su.se/ to locate the prepro sequence.

Polystyrene-Binding Peptide
Source: Synthetic For background, read PMID: 19056299. Make a BglBrick part encoding the peptide: FKFWLYEHVIRG no start, no stop, in frame.