User:Howard Boland/Notebook/Art from Synthetic Biology/2010/08/23

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Overnight Growth
I put on overnight growth of nine 10ml bottles using 10µl of 50mg/µl Kanamycin stock.

Colonies were picked from the overnight plate growth:
 * 1) 4 colonies from the previous working pAU66katE#A1#2 plate
 * 2) 1 colony from pUA66katE#A1, SAP
 * 3) 1 colony from pUA66katE#B1, SAP
 * 4) 1 colony from pUA66katE#Diff, SAP
 * 5) 1 colony from pUA66katE#1, Normal Ligase
 * 6) 1 colony from pUA66katE#2, Normal Ligase

The bottles were placed in a shaker at 37ºC and grew overnight


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