IGEM:PennState/Labbook/GalenLynch/2007-7-16

July 16th, 2007: 10:00-4:15
 * Prepared DNA samples for sequencing
 * Quantified mini-prep DNA
 * Samples I741011, I741018
 * Made fresh SYBR green I 10x stock
 * Disposed of old ones
 * Last of I741015 and I741005 accidentally used in restrictions
 * Since all composite parts were made with these particular colonies, it's necessary to salvage the DNA
 * To reclaim DNA from restriction reaction: Denature EcoR1 at 65 C for 20 minutes, Ligate, and transform