User:Tk/Notebook/MF/2010/06/12

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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Desired parts

 * Mflorum optimized genes
 * CFP
 * YFP
 * RFP
 * LacZ
 * cat (probably from Sebastian)
 * I-sceI enzyme
 * Cre-lox sites (maybe from Sebastian)
 * I-sceI cut site
 * E. coli optimized mfl304
 * modified pheS (already exists?)
 * up and down sequences around RE gene sites


 * modified plasmid containing left and right transposon sites (M1000 and M1001) with pvuII sites

Eliminate sites for PvuII, NheI, bibrick sites,


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