840:153g:Projects/project3/2009/03/03

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Tear Inducing Bacteria
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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March 3, 2009

 * We ran a gel to confirm that high molecular DNA was extracted from our onion. We had 8 DNA samples that were combined into one tube and 10 microliters of RNase A was added.  PCR was then run using our two different primers and 5 different gradients.  The next step is to run a gel to confirm PCR worked on Thursday.


 * We also ran a gel for our plasmid DNA to confirm that we had the correct plasmids. We digested the DNA with Xba I and Pst I.  Our gel was facing the wrong direction and so it did not run correctly.  Therefore, we will have to redo this procedure on Thursday.




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