IGEM:Harvard/2006/Cyanobacteria/Building cyanobacteria incub

The below directions were assembled by the Harvard iGEM team; questions can be sent to contacts here.

Materials and Methods
Note: All of the above materials, except for the light meter and incubator, can be found at Home Depot.
 * 1) Cool Fluorescent Lights, 40W or higher
 * 2) *Used Philips Circline Cool White 30cm 32W + 20cm 22W
 * 3) *To mount, Lithonia Lighting #FM BLWH
 * 4) Digital Timer with ground
 * 5) *Intermatic Timer DT27C
 * 6) Control of light intensity
 * 7) *Window screen
 * 8) *Flourescent light cover - also distributes light
 * 9) Light meter which can read from 0-10000 lux
 * 10) *VWR 62344-944
 * 11) Incubator with temperature control
 * 12) *Innova 4230

Protocol

 * 1) Clean materials using EtOH
 * 2) Set up light fixture
 * 3) Wire end to the outlet jack
 * 4) Program timer to 14h light / 10h dark
 * 5) Ensure light intensity of around 4200lux, ranged up to 8000lux.