IGEM:Cambridge/2008/Electric Output

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=Aim= To create a system which responds to ligand binding with a detectable voltage caused by a K+ flux.

=Background= [[Media:Voltage_project.ppt | Presentation]]

=Current Progress=

Progress Notebook

=Next Steps=

Characterise promoters

1. Simulate and design   ‘reporter plasmids’ with correct biobricks restriction enzyme sites

2. For each strain:   2 plasmid backbones


 * PSC101 (A) with   OsmY (promoter) + RFP + Stop


 * Another (B), not   yet defined, with Bba_J23100 + YFP + Stop


 * Tests for each strain : plasmid A, plasmid B, plasmid A + plasmid B (so 15 tests)

3. Quantify transformation   efficiency (colony counter)

4. Quantify promoter   strength (light intensity, expression levels)

=Useful Links=

Protein prediction tools

Uniprot database

=Literature=

Kdp operon diagram

Kdp plasmid

The Kdp-ATPase system and its regulation

Potential Chassis: Strain JW1242-1 Strain JW0710-1

Kdp mutant - paper from 1971

Worldwide E.coli Databases

Characterisation of kdpD - 2005