User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/23

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04/23/10

 * &#x2713; Minipreps: KAH154-159/MV2
 * &#x2713; Sequencing: KAH156, 157/MV2

Minipreps > Check with XbaI digests

Sequencing (Genewiz, standard) --> Can't sequence old KAH156/MV2, used up last of it in transfection --> Need to confirm 156, 157 in order to have proper id for transfected cells


 * 1) KAH156/MV2 (new prep) DDB122: fshPcCD! (should be flyPcCD)
 * 2) KAH156/MV2 (new prep) DDB123: fshPcCD! (should be flyPcCD)
 * 3) KAH157/MV2 (new prep) DDB122: flyPcCD! (should be fshPcCD)
 * 4) KAH157/MV2 (new prep) DDB123: flyPcCD! (should be fshPcCD)
 * 5) KAH157/MV2 (old prep) DDB122: bad seq
 * 6) KAH157/MV2 (old prep) DDB123: flyPcCD! (should be fshPcCD)

--> Looks like fly and fish PcCD's were mixed up during cloning. Take note of this and continue. May want to check other transfection constructs (including VP64+ set) just to be sure the id's of the stable lines are correct.


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