IGEM:British Columbia/Protocols/Amplifying BioBrick Parts

Materials

 * 2009 Spring Distribution plates
 * Competent cells
 * sdH2O
 * LB agar plates (with appropriate antibiotics)
 * LB broth (with appropriate antibiotics)

Equipment

 * miniprep kits (1 prep/part)

Method

 * With a pipette tip, punch a hole through the foil cover into the well corresponding to the part you want. Make sure that the plate is properly oriented
 * Resuspend in a PCR tube with 15uL of sdH2O (by pipetting in and out of the water).
 * Transform competent cells with 1uL of the resuspended DNA, plate bacteria with the appropriate antibiotic, and grow overnight.
 * Pick a single colony and inoculate ~3.5-6mL of medium (with the correct antibiotic) and grow overnight (16-18 hours).
 * Make a glycerol stock using 0.75mL of culture.
 * Miniprep the DNA using ~2.5-5mL of culture
 * Label well and store at -4°C.