IGEM:The Citadel/Notebook/Notes Brian/08-05-10

Biobrick construction map:

0.1 E0240 (A)[E,P] + J04450(T)[E,P] = J23102 (T, ex A) 0.2 B0015 (A)[E,P] + J04450(T)[E,P] = B0015 (T, ex A) 0.3 C0160 (A)[E,P] + J04450(T)[E,P] = C0160 (T, ex A) 0.4 J23102 (A) + E0240 (T, ex A) + J04450(C) = ~J02E40 (C) [3A ASSEMBLY TEST] 0.5 J23102 (A) + F1610 (T, ex A) + J04450(C) = ~J02F10 (C) [QS Stage 1] 0.6 ~J02F10 (C) + F2620 (A) + J04450(T) = ~J02F10F20 (T) [QS Stage 2] 1.0 ~J02F10F20 (T) + E0240 (A) + J04450(C) = ~J02F10F20E40 (C) [QS TEST] 2.0 I746350 (A) + B0015 (T, ex A) + J04450(C) = ~I50B15 (C) 2.1 ~I50B15 (C) + I746360 (A) + J04450(T) = K274370 (T) 2.2 ~I50B15 (C) + I746361 (A) + J04450(T) = K274371 (T) 2.3 ~I50B15 (C) + I746364 (A) + J04450(T) = K274374 (T) 2.4 ~I50B15 (T) + I746365 (A) + pSB1C3 = K274375 (C) 3.0 I746351 (A) + B0015 (T, ex A) + J04450(C) = ~I51B15 (C) 3.1 ~I51B15 (C) + I746360 (A) + J04450(T) = K274380 (T) 3.2 ~I51B15 (C) + I746361 (A) + J04450(T) = K274381 (T) 3.3 ~I51B15 (C) + I746362 (A) + J04450(T) = K274382 (T) 3.4 ~I51B15 (C) + I746364 (A) + J04450(T) = K274384 (T) 4.0 I746352 (A) + B0015 (T, ex A) + J04450(C) = ~I52B15 (C) 4.1 ~I52B15 (C) + I746361 (A) + J04450(T) = K274391 (T) 4.2 ~I52B15 (C) + I746362 (A) + J04450(T) = K274392 (T) 4.3 ~I52B15 (C) + I746364 (A) + J04450(T) = K274394 (T) 4.4 ~I52B15 (C) + I746365 (A) + J04450(T) = K274395 (T) 5.0 F2620 (A) + K274382 (T) + J04450(C) = ~F20K82 (C) 5.1 ~F20K82 (C) + E0240 (A) + J04450(T) = ~F20K82E40 (T) 5.2 ~J02F10 (C) + ~F20K82E40 (T) + J04450(K) = ~J02F10F20K82E40 (K) [QS & ST TEST] 6.0 B0034 (A) + C0160 (T, ex A) + J04450(C) = ~B34C60 (C) 6.1 ~B34C60 (C) + B0015 (T, ex A) + J04450(K) = K218026 (K)

For tomorrow:

0.1 E0240 (A)[E,P] + J04450(T)[E,P] = J23102 (T, ex A) 0.2 B0015 (A)[E,P] + J04450(T)[E,P] = B0015 (T, ex A) 0.3 C0160 (A)[E,P] + J04450(T)[E,P] = C0160 (T, ex A)

For Saturday:

0.4 J23102 (A) + E0240 (T, ex A) + J04450(C) = ~J02E40 (C) [3A ASSEMBLY TEST] 0.5 J23102 (A) + F1610 (T, ex A) + J04450(C) = ~J02F10 (C) [QS Stage 1] 2.0 I746350 (A) + B0015 (T, ex A) + J04450(C) = ~I50B15 (C) 3.0 I746351 (A) + B0015 (T, ex A) + J04450(C) = ~I51B15 (C) 4.0 I746352 (A) + B0015 (T, ex A) + J04450(C) = ~I52B15 (C) 6.0 B0034 (A) + C0160 (T, ex A) + J04450(C) = ~B34C60 (C)