IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/02

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Test of purification plasmid kit by Roche
The gel looked like there were genome DNA
 * Pato and I follow the steps described by the protocol's kit, in order to isolate the plasmid pBBRMSC5 which was in E. coli
 * We did an electroforesis with a 1% agarose gel, 80 V X 100 min, 3 microL dye and 5 microL DNA

The gel show the two bands corresponding to the chromosome, which were charged in two lanes:
 * With the same isoleted plasmid, we did another electroforesis in order to check if the features of it were the cause of the bad result. 125 V X 30 min, 3 microL dye and 10 microL DNA


 * }