Knight:Loading dye

Description
6X loading dye to mix with DNA samples for use in agarose gel electrophoresis.

Components

 * Ficoll 400
 * Deionized water
 * Orange G dye

Procedure
Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4&deg;C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.