Jessica Karen Wong/Notebook/2007-7-26

Cleaning etc

 * PCR cleaned all overnight digests - CCDB-E/X, CCDB-S/X, I2055-M/N, 3K3-E/P, 1AK3-E/P, 1AK3-E/X
 * Minipreped 3K3 to have a DNA stock
 * Made new Measurement Kit Primers box for the -20



I2055

 * Ran gel of overnight col pcr's
 * Only colony # 17 looks good
 * Overnighted I2055-3K3 #17
 * Ligated newly digested I2055 into the newly digested plasmids
 * Ligated I2055-3K3, I2055-1AK3
 * Transformed 3ul ligation and plated

I2056

 * Overnight transformation plate had no colonies
 * Ligated I2056-1AK3, I2056-3K3 w/ newly digested plasmids
 * Transformed and plated

E0240

 * Ligated E0240-1AK3
 * Transformed and plated
 * Set up 100ul BB PCR's of E0240-3K3 E/X and E0240-3K3 E/S at 52
 * Ran gel of BB PCR, both look good (1st 2 bands after ladder)
 * PCR cleaned both E0240-3K3-X and E0240-3K3-S
 * Digested both BB PCRs w/ Not1 in buffer 3 overnight

I2057

 * Ligated I2057-1AK3, transformed and plated
 * BB PCRed I2057-3K3 E/X and I2057-3K3 E/S at 52
 * Ran gel of BB PCR - both looked good (2 farthest right samples)
 * PCR cleaned both BB pcr's
 * Digest both w/ Not1 in buffer 3 overnight

T9002

 * Overnight plates of T9002-1AK3 and T9002-3K3 have 4 colonies each
 * Set up 10ul Col PCR's
 * Ligated F2620-1AK3 w/ new plasmid, transformed and plated
 * Ran out of cut F2620
 * Digesting F2620 - M/X in buffer 4 overnight