User:Anthony Salvagno/Notebook/Research/2011/01/24/pBSTXI PCR

Preliminary Work

 * Make thermocycler program.
 * extend for 5 min
 * anneal at 60C
 * dilute some plasmid DNA
 * currently at 100ng/ul
 * make 1.5ng/ul
 * take 5ul and add 333ul to it
 * dilute primers
 * currently at 50uM and 187uM
 * make 10uM each
 * 1:5 and 1:18.7 respectively

Reaction Program
I created a new program in the thermal cycler. The program is shown in the reaction spreadsheet above, but here are the specifics anyways:
 * program name is PBSTXI
 * it is set to anneal at 60C
 * it is set to extend at 72C for 5 min. This is per the recommendation of 1min per 1kb of DNA, we have 4.5 kb so this should be alright.