User:Karmella Haynes/Notebook/BioBrick cloning/2009/11/30

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11/30/09

 * &#x2713; Minipreps: KAH99(2), 100(2), 101(2), 102(2), 103(2), 104(2)
 * &#x2713; Assemblies: KAH011; KAH93, 94, 95 (RFP miRNA reporters) into pcDNA3.1+puro/ΔCMV (MV2)

Minipreps > Check with E/P digests, E/P/ApaLI for KAH99, 100, 101 > ApaLI fragments = 1246, 776, 745, 497

Assemblies
 * 1) KAH011: NLS-myc-stop (KAH011 dsOligo)/(X/P)/64 &#x2713; + V0120/(X/P)/3200 &#x2713;
 * 2) KAH93/MV2: (1) KAH93/(X/P)/4839 + pcDNA3.1+puro/ΔCMV(MV2)/(S/P)/4529 &#x2713;
 * 3) KAH94/MV2: (2) KAH94/(X/P)/5011 + "
 * 4) KAH95/MV2: (3) KAH95/(X/P)/5083 + "

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

--> * Dilute MV2 1:5 w/ dH2O


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