Registry/Measurement kit/Notebook/2007-7-3

Sequence verification

 * Notes
 * Use AlignX for multiple sequence alignment
 * Sequences in Documents -> sequences -> folder named w/date
 * View chromatograms in FinchTV
 * Registry also has sequence verification software in development.
 * I2055 sequence didn't check (R0040 missing)
 * This explains why the primers for the PCR didn't work...
 * Choosing colonies 3,4, and 6 to sequence, since they looked slightly larger on the 6/19 gel.
 * Growing with amp+kan
 * Also ordered primer to insert the sequence by PCR:
 * format: 8-mer.MfeI.promoter+mix.part of gfp (Tm)
 * CTTAGTAG.CAATTG.tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag.ATGCGTAAAGGAGAAGAACTTTTC (52.4)

I2056 Construction

 * Got one colony on 1AT3
 * Re-plating the rest (1AT3, 1AC3)
 * Did colony PCR, but reaction seems to have evaporated...diluting w/10uL water and running on gel. re-doing PCR in parallel

Analytical Gel

 * Loaded in this order: ladder, blank, t9002 scarred, I2056, ladder.

 2 log ladder