Talk:Knight:Beta-galactosidase assay

500mM dibasic sodium phosphate
7.098g Na2HPO4 in 100mL H2O

1% hexadecyltrimethylammonium bromide (CTAB)
100mg CTAB in 10mL H2O

1% sodium deoxycholate
10mg sodium deoxycholate in 1mL H2O

Light-sensitive. Store at 4&deg;C.

1M sodium carbonate

 * 52.995 g Na2CO3 in 500mL H2O

Measuring repression as a function of A600

 * 1) Grow an overnight culture of P20060.E0433
 * 2) Dilute back to A600 = 0.001 (as measured on the Nanodrop) in two tubes (1mL each)
 * 3) Let grow for 1 hour
 * 4) Prepare the permeabilization solution and aliquot out 80 &mu;L per microfuge tube.
 * 5) Add 20 &mu;L of 50 mM IPTG to both tubes and 1 &mu;L AHL to one tube.
 * 6) Every hour, measure the A600 and add 20 &mu;L to 3 microfuge tubes.
 * 7) Proceed with the Knight:Beta-galactosidase assay as normal.
 * 8) Plot the Miller Units as a function of A600.