User:Sarah Signor/Notebook/Genetics of Pigmentation/2011/01/07

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January 7th
*This is almost exclusively a day of bench work, trouble shooting library construction for the GA


 * I am troubleshooting the labs RAD tagging protocol. Currently investigating the possibility that cDNA doesn't work with this method/restriction enzyme.


 * I am, however, also still trying to get the barcoded adapters to work.


 * Today I started all four libraries beginning with end repair and a-tailing (RAD, Genomic, Barcoded, illumina). I am using the genome center protocol rather than the one made available along with the Baird et al. 2008 paper.


 * Ondrej may have been following that protocol up to this point when he left me his samples, but from this point on I see few differences between the two. I can't continue with adapter ligation until he clarifies the labels on the tubes.


 * I have also finished formalizing my QTL analysis of the D. malerkotliana malerkotliana and D. malerkotliana pallens data, complete log of the rQTL commands and output can be found in the file qtl_notes_tryingtodefineinterval.txt, but this doesn't include notes on building the genetic map.


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