IGEM:Peking University/2008/Experiment/PKU 0620 G2 1

Primer design

 * Primer 1
 * Primer name: F-HindIII-Apal-Gal4
 * Sequence(5'to3'):CCCAAGCTTGGGCCCAAGATGAAGCTACTGTCTTCTATCGAACAA
 * Tm:64.9°C
 * Primer 2
 * Primer name: R-EcoRI-Spel-Gal4
 * Sequence(5'to3'):GGGGAATTCACTAGTATTTTACTCTTTTTTTGGGTTTGGTGGGGT
 * Tm:61.3°C

PCR

 * conditions


 * Enzyme cutting-pPT1-XbaI-20 μL