User:Karmella Haynes/Notebook/Polycomb project/2010/08/02

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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08/02/10

 * &#x2713; H3me Reporter clone screening: cells are too crowded for transfection, split 1:4 for transfection tomorrow (12-well plates, ab-free, 1 mL per well); make back-ups in 6-well plates (FTRx-hygro maintenance medium)
 * &#x2713; KAH131 screening: check RFP expression, expand selected clones
 * &#x2713; Thaw: KAH126-3 for repeat of senescence expt.
 * &#x2713; Microscopy: finish Pc-ATF images if DAPI and RFP signal still good

KAH131 screening > RFP expression -dox/ vs. +dox --> Note: These two and four other RFP+ clones showed lots of cell death after dox induction (~4 days); FlyPc-RFP-VP64 appears to be highly toxic --> Expand two lines in 6-well plates (FTRx+puro maintenance medium) --> Might omit this line from further studies if difficult to grow
 * 1) KAH131-4 (+/++)
 * 2) KAH131-8 (+/++)


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