Acetobacter Xylinum Culture

Overview
General guidelines on how to grow up a culture of Acetobacter xylinum, ATCC strain 53582.

Preparation of Acetobacter Media
To prepare ∼500 ml of liquid Acetobacter media, add the following:
 * Glucose - 10 g
 * Peptone - 2.5 g
 * Yeast extract - 2.5 g
 * Na2HPO4 - 1.35 g
 * Citric acid - 0.75 g
 * Distilled water - 500 ml
 * If you are making plates, use the same protocol but add 7.5 g of agar.

Procedure

 * 1) Prepare media as outlined
 * 2) Autoclave to sterilize media.
 * 3) Streak/inoculate Acetobacter onto plates or in media.
 * 4) Incubate cells at 26°C for 2-3 days.
 * 5) If using a freeze dried source of Acetobacter (ex. ATCC shipment), growth may take up to 4 days.

Contact

 * morto077@uottawa.ca

or instead, discuss this protocol.

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