User:Tk/Notebook/MF/2008/07/13

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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 * style="background-color: #F2F2F2" align="center"|  |Main Page


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More transformations

 * 10 ml cultures harvested and wash 2x with EPB, resuspended to 1200 ul in EPB
 * mixed with 16 ul of old transposome, 143 ng/ul; also added 8 ul of each new SB transposomes
 * mixed 80 ul 1 M sorbitol
 * 4 1mm cuvettes, 1.5 KV, one arc'd and was discarded
 * Added 1 ml 1161 (no tet)
 * incubate 55 min, slight color change
 * plate on 3 x 3 plates -- washed cuvettes with 200 ul 1161 and plated that also

Dishwasher is busted

 * again! no wash motor action, no heat


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