User:Karmella Haynes/Notebook/Polycomb project/2010/09/09

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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09/09/10

 * &#x2713; p16 time course: Induce 4-day samples with 1 μg/mL dox (3 pm)
 * &#x2713; Order oligos: new RT-PCR primers for HHEX
 * &#x2713; Plasmid cultures: pick three colonies each from KAH160/015 and KAH170/015 plates (8/07/10)

Pc-ATF expression in other cell lines > Casey can get expression from KAH165/015 but not from KAH160/015 and KAH170/015 in HepG2 cells --> Mutation in the viral backbone? > Pick three colonies each from original cloning plates > Tomorrow: order sequencing using Genewiz primers:
 * 1) CMV-forward: reads into HIV-1 5' LTR (upstream)
 * 2) BGHR: reads into HIV-1 5' LTR (downstream)


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