IGEM:PennState/Labbook/NoahJohnson/2007-9-20

September 20, 2007


 * DH5a competent cell prep


 * Ligation labelling system:
 * 1: Tube 1 crp* & Tube 1 psB1A2 (Fridge ligation)
 * 2: Tube 2 crp* & Tube 4 psB1A2 (Fridge ligation)
 * 3: (-) Tube 1 psB1A2 (Fridge ligation)
 * 4: Tube 3 crp* & Tube 1 psB1A2 (Benchtop ligation)
 * 5: Tube 4 crp* & Tube 4 psB1A2 (Benchtop ligation)
 * 6: (-) Tube 4 psB1A2 (Benchtop ligation)


 * Transformation:
 * 3uL ligation mixture in 50uL DH5a competent cells
 * Will do 2 transformations for each ligation, one with todays competent cells (New) and one with yesterday's (Old).
 * Plated on LB Amp


 * Made 500mM filter-sterilized glucose soln for SOC:
 * 0.9g Glucose (MW=180.16 g/mol) added to 10mL dH20
 * Filter sterilized and put in 15mL Falcon tube
 * Can be found in rack in fume hood

6-11:30