IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week1

/Chemical and Light/ /Widgetry/

=Monday Meeting Notes=

Light team

 * knockout protocol
 * cloning part

Chemical team

 * transform registry DNA into Shewie
 * confirm Shewie growth via PCR

Widget team

 * create basic MFC protocol
 * speak with Pete and confirm design
 * think about computer interfacing
 * LabView/MatLab?
 * Buy computer?

Team Goals

 * anaerobic Shewie?
 * manganese oxide?
 * meet with Diane Newman

Mutants from Orianna

 * mtrA
 * mtrB
 * both

UT Austin

 * OmpR knockout

Protocols

 * Homologous recombination (Pacific Northwest?)
 * Cre-Lox (Orianna)
 * Colorimetric assay (Orianna)
 * Shewie transformation (Diane Newman)