User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/29

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Chelex Yeast Extraction
1) Pellet 25.0ul of growing culture (1min at 15,000rpm) 2) Remove supernatent 3) Add 100.0ul of Chelex 4) Vortex and spin down 5) Boil for 10min 6) 1 min at 27C (to prevent lids from popping when opening) 7) Vortex and spin down at 15,000rpm for 1 min before use

MATA2 Yeast Amplification
Load 2.0ul sample