IGEM:PennState/Labbook/NoahJohnson/2007-7-23

July 24, 2007
 * Added to each tube:
 * 5uL 10X Pfx Amplification buffer/ ThermoPol
 * 1.5uL 10mM dNTP
 * 1uL 50mM MgSO4
 * 1.5uL Primer Mix (0.75uL each)
 * 1uL Template DNA (10pg-200ng)
 * 0.5uL Pfx DNA Polymerase/ TAq Polymerase
 * 39.5uL Autoclaved dH2O (Enough to make 50uL total)


 * Template DNA Used:
 * 1) I741015
 * 2) I741017
 * 3) I741018
 * 4) I741019
 * 5) I741020
 * 6) I741021
 * 7) I741011
 * 8) I741023
 * 9) I741005
 * 10)   (-)


 * Primers:
 * 1) 3398 & 3399
 * 2) 3400 & 3401
 * 3) 3406 & 3407
 * 4) 3408 & 3409
 * 5) 3402 & 3403
 * 6) 3404 & 3405
 * 7) 3412 & 3413
 * 8) 3410 & 3411
 * 9) 3416 & 3417
 * 10) 3410 & 3411


 * Thermocycler Protocol (pfx)
 * Cycle 1: (1x)
 * Step 1: 94°C for 2:00
 * Cycle 2: (28x)
 * Step 1: 94°C for 0:15
 * Step 2: 54°C for 3:00
 * Step 3: Gradient for 1:00
 * H . .  G  . . .  F  . .  E  . . .  D  . .  C  . . .  B  . .  A
 * 60° 60.8° 61.9° 63.5° 65.8° 67.5° 68.5° 69°
 * Cycle 3: (1x) Hold at 4°C for ∞


 * PCR Start: 1:09 PM
 * PCR End:  2:35 PM

9:30-5