User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/01

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 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Beginning with the transformation
   -NaCl -> 30g -Glycerol -> 1g -Bacto-Peptone > 10g -Beef Extract -> 3g 
 * Today, I transformed competent cells with the plasmid that I constructed (the ligated luciferase with the double terminator). After following the protocol, I incubated the plates (LB with ampicilin)at 37°C ON.
 * I also did BOSS medium for a Vibrio fischeri assay that we will held for a total of 1L (500ml liquid medium and 500ml solid agar).
 * According to the protocol, to obtain solid medium you should add 15 g of Bacto-Agar, dilute the ingredients in water (pH should be about 7,3), and fill with H2O up till 1000 ml.


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