IGEM:PennState/Labbook/NoahJohnson

Noah Johnson


 * Penn State iGEM 2007
 * [iGEM 2007]
 * Penn State Undergraduate - BioEngineering
 * Email [mailto:nrj5011@psu.edu nrj5011@psu.edu]
 * Phone (814) 441 2037

Calender name=IGEM:PennState/Labbook/NoahJohnson date=2007/04/01 view=threemonths format=%name/%year-%month-%day weekstart=7

name=IGEM:PennState/Labbook/NoahJohnson date=2007/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7

name=IGEM:PennState/Labbook/NoahJohnson date=2007/10/01 view=threemonths format=%name/%year-%month-%day weekstart=7

name=IGEM:PennState/Labbook/NoahJohnson date=2008/01/01 view=threemonths format=%name/%year-%month-%day weekstart=7

name=IGEM:PennState/Labbook/NoahJohnson date=2008/04/01 view=threemonths format=%name/%year-%month-%day weekstart=7

[April 30, 2007-Bacterial Conjugation Presentation April 30, 2007 ]  [Project Proposal: Bacterial Conjugation Close Project Proposal: Bacterial Conjugation ] 

[May 24, 2007-Restriction Digest May 24, 2007 ]  [Restriction Digest Close Restriction Digest ] 


 * 1) Insert A,B,C
 * 2) *3.5 H20
 * 3) *3.5 DNA (J23038: A (LW0006 LW-1),B (LW0007 LW-1),C (LW0008 LW-1))
 * 4) *1 EcoR1 Buf
 * 5) *1 BSA
 * 6) *0.5 EcoR1
 * 7) *0.5 SpeI
 * 8) Vector A,B,C
 * 9) *3.5 H20
 * 10) *3.5 DNA (J31004: A (LW0003 LW-1),B (LW0004 LW-1),C (LW0005 LW-1))
 * 11) *1 EcoR1 Buf(!WRONG:Should have been Buffer 2!)
 * 12) *1 BSA
 * 13) *0.5 EcoR1
 * 14) *0.5 XbaI
 * 15) Vector -
 * 16) *6.5 H2O
 * 17) *3.5 DNA (J31004: A (LW0003 LW-1))
 * 18) EcoR1 Vector
 * 19) *4 H2O
 * 20) *3.5 DNA (J31004: A (LW0003 LW-1))
 * 21) *1 EcoR1 Buf
 * 22) *1 BSA
 * 23) *0.5 EcoR1
 * 24) XbaI Vector
 * 25) *4 H2O
 * 26) *3.5 DNA (J31004: A (LW0003 LW-1))
 * 27) *1 Buffer 2
 * 28) *1 BSA
 * 29) *0.5 Xba
 * 30) SpeI Vector
 * 31) *4 H2O
 * 32) *3.5 DNA (J31004: A (LW0003 LW-1))
 * 33) *1 Buffer 2
 * 34) *1 BSA
 * 35) *0.5 SpeI

[May 30, 2007-Biofilms Presentation May 30, 2007 ]  [Project Proposal: Biofilms <span class="_toggle _toggler" style="display:none;">Close Project Proposal: Biofilms ] <div class="_toggle" style="display:none;">

[<span class="_togglegroup _toggle _toggler">June 4, 2007-iGEM Meeting Presentation <span class="_toggle _toggler" style="display:none;">June 4, 2007 ] <div class="_toggle" style="display:none;"> [<span class="_togglegroup _toggle _toggler">iGEM Meeting Presentation <span class="_toggle _toggler" style="display:none;">iGEM Meeting Presentation ] <div class="_toggle" style="display:none;">

[<span class="_togglegroup _toggle _toggler">June 11, 2007-Primer Sequencing <span class="_toggle _toggler" style="display:none;">June 11, 2007 ] <div class="_toggle" style="display:none;"> [<span class="_togglegroup _toggle _toggler">XylA,B Primer <span class="_toggle _toggler" style="display:none;">XylA,B Primer ] <div class="_toggle" style="display:none;">

3725940-3728788 Found on Ecocyc

TTACGCCATT AATGGCAGAA GTTGCTGATA GAGGCGACGG AACGTTTCTC GTCGTGGCTG ATAAGCGGCA TAACGCTGCG CATCTGGTAG ATGCGACTGT TCTAACGGTA GTTGCGGCAA CAATTCAATG AGCGATTTCT CTGGATTCGC CGCGATCTGC GCCAGCCTTG CTGCGCCCAG TGCTGGCCCC ACATCCCCCC CCGTACGGTA ATCGAGCTGC TGACCGCTGA TATCCGCCAG CATCTGACGC CAGTACTCAC TACGCGCCCC GCCCCCAATC AACGTAACAC TTTGCGGTTT AATACCGCAG GCATGCACGA CATCCATGCC ATCTGCCAGC GCATAACCCA CGCCTTCCAG CACTGCTCGC GCCAGTTCAT TGGGGCCATG TTGATGAGTC AAACCAAAGA AAACCCCCTT CGCCTGGGGA TTATTGTGTG GCGTACGCTC GCCGGAAAGA TAAGGCAGAA ACCAAACTGG CTCGGCACTT TCATCAGCCT GTTGAGCTGC AGCGATTAAA GCTGGGACAT TGCTCAGGCC GGTTAATTTC GCGGCCCAAT CCAGACACGA CGCTGCACTC AGCATCACAG ACATTAAATG CCAACGTTGC GGTAGCGCAT GGCAAAAGCT ATGTACGGCG CTTTCTGGCT TGCTTAAGAA CCCTTCGCTG ACAGCAAAAT AGACCCCCGA CGTCCCCAGC GATAACATTG CCTGATTAGC ATCAACCATT CCCACACCAA CTGCACCAGC TGCATTGTCG CCACCGCCTG CGACAACTGG CACCGTCGCC ATACCCCACG CTTTCGCAAC TTCAGGTAAC AAAGCACCAG TAATTTCGCT GCCTTCGTAT AATGCGGGCA TCTGGTCACG AGATAAGTCG CAAGCCTGCA GCATGACGTC ACTCCAGTCA CGCTTTGCGA CATCCAGCCA CATGGTGCCA GCTGCGTCAG ACATATCGCT GGCAAACTCC CCCGTCATAC GCAGACGCAA GTAATCTTTC GGTAATAATA CTTTGTCGAT TTGACGGAAT ATCTCCGGCT CATGCCGCTG AACCCATAGC AATTTAGGCG CAGTAAATCC GGGCATCATC AGGTTGCCGG TAATCACCCG CGATTGCGGA ACTCGCGCTT CCAGCAAAGT GCACTCTTGC GCACAGCGCC CGTCGTTCCA CAAAATGGCA GGGCGTAACA CCCGTTGCTG AGCATCCAGC AAGGTTGCTC CGTGCATCTG GCCGGCAATA CCCAATGCTT TAACGTCCTG CAGAGAATGC TGATCGCCCA GAGCTTTCAT TGCGCGATCA GTTGCCTGCC ACCACTGTTC CGGGTCTTGT TCCGACCAGA GTGGATGCGG GCGCGAAACG GTCAGCTTTT CCGTTTGCGC AGCAACCACC TCACCCTGCT CGTTGAGCAA AATAACTTTT ACGCCCGAGG TGCCAAGATC TATCCCGATA TACATATCGA TCGTTCCTTA AAAAAATGCC CGGTATCGCT ACCGATAACC GGGCCAACGG ACTGCACAGT TAGCCGTTAT TTGTCGAACA GATAATGGTT TACCAGATTT TCCAGTTGTT CCTGGCGACC ACTCTGATGC ACCGGAGACA AATGATGTTC CTGAGCATAT TTGGCTAAAT CTGCCAGTGA CATTTGGCCT TTCAGGATTT GCTGGCCCAA TTCGCTATTC CAGCCGGAAT AACGCTGCGC GATGCGTTTA TCCAGCTCGC CATCTTCAAT CATGCGCGCT GCAATTTTCA GCGCCAGTGC CATCGTATCC ATCGCGCCGA TATGACCGTA AAACAGATCA TATTTATCAG TACTTTGACG ACGTACTTTG GCATCGAAGT TCAGACCACC GGTGGTGAAA CCGCCTGCTT TGAGAATTTC ATACATCACC AGCGCATTCT CTTCCACACT GTTCGGGAAC TGGTCGGTGT CCCAGCCCAG TTGCGCATCG CCACGGTTGG CGTCGACAGA ACCGAACAGG CCAAGCGCAA TGGCGGTGGC TATTTCATGA TGGAAAGAGT GACCTGCCAG CGTCGCGTGG TTAGCTTCAA TGTTCAGTTT AATCTCTTTT TCCAGACCAA ACTGTTTCAG GAAGCCATAG ACCGTCGCGG CATCGTAATC ATATTGATGT TTGGTCGGTT CTTGCGGTTT CGGTTCGATA AGCAACGTGC CCTGGAAACC GATTTTATGT TTATGCTCAA CCACCATCTG CATAAAGCGG CCCAGTTGTT CACGCTCCTG ACGCAAGTCG GTATTTAACA GCGTTTCGTA ACCTTCACGA CCGCCCCACA GGACATAGTT TTCACCGCCC AATTTATGGG TTGCTTCCAT CGCTGTAACA ACTTGCGTTG CCGCCCAGCT GAAGACTTCA GGATCTGGGT TCGTCGCCGC ACCCGCGCCG TAGCGAGGGT TTGTAAAGCA GTTGGCCGTT CCCCACAGCA GCTTCACGCC GCTCTCTTCT TGCTTGCCTG CCAGGACATC AACCATTTGC GCAAAATTAT TGATGTACTC TTTTAACGAC GCGCCCTCAG GGGAAACATC CACATCGTGG AAGCAATAAA ATGGCACATG TAACTTGTGG AAAAACTCAA ATGCGACATC TGCTTTACGC TTCGCCAACG CCAGTGCCTC ACCAGGCTGC TGCCACGGAC GATTAAACGC CCCCACACCA AACATATCCG CCCCGTTCCA GCAGAAGGTG TGCCAGTAGC AGGCGGCAAA ACGCAAGTGC TCTTCCATAC GCTTACCCAA CACCAGTTCG TCGGGATTGT AGTGACGGAA TGCTAACGGG TTTGAGGATT TTGAGCCTTC ATAACGAACG CGATCGAGCT GGTCAAAATA GGCTTGCAT

Left side primer: gaattcgcggccgcttctagag TTACGCCATT AATGGCAGAA

Right side primer: ctgcagcggccgctactagta ATGCAAGCCT ATTTTGACCA

[<span class="_togglegroup _toggle _toggler">XylR Promoter Region Primer <span class="_toggle _toggler" style="display:none;">XylR Promoter Region Primer ] <div class="_toggle" style="display:none;">

3732905-3733001 Found on Ecocyc

CAACCAAACG CCGTTCTTGA TTTTGATAAA AATTTTCTCA AAGCCGGTTA CGTATTACCG GTTTTGAGTT TTTGCATGAT TCAGCAGGAA AAGAACC

Left side primer: gaattcgcggccgcttctagag CAACCAAACG CCGTTCTTGA

Right side primer: ctgcagcggccgctactagta GGTTCTTTTC CTGCTGAATC