User:Karmella Haynes/Notebook/Polycomb project/2010/06/03

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06/03/10

 * &#x2713; Transfection: histone methylation reporter

Lipo transfection > KAH96 [5xGal4 : HSVtkTATA : K : YFP : YFP : NLSstop2 : polyA : HS4 : HS4] > KAH142 [DPRE : 5xGal4 : HSVtkTATA : K : YFP : YFP : NLSstop2 : polyA : HS4 : HS4]

> Add Lipo to 250 μL Opti-MEM --> R.T/ 5 min. > Add DNA to 250 μL Opti-MEM > Add DNA mix to Lipo mix --> R.T./ 20 min. > Add 500 μL complexes to each well (4 ml med. total each); Grow cells at 37&deg;C > Refresh medium after 5 hours (ab-free)


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