User:Karmella Haynes/Notebook/Polycomb project/2010/03/19

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

03/19/10

 * &#x2713; cDNA: 8-day dox induction Pc-ATF cell line set
 * &#x2713; Cell cultures: Refresh medium for KAH126, 130, 132 starter cultures (20% FBS)

RNA prep/ cDNA synth.

> RNA Preps (yesterday) --> Frozen: 1 mL TriZol/ well (6-well dishes) --> Resuspend RNA pellet in 10 μL THE RNA Storage Solution

> oligo(dT) Primer annealing

> cDNA synthesis mix --> 8 reactions total

--> Add 10 μL mix to each annealing rxn. --> 50°C/ 50 min., 80°C/ 5 min., ice --> Add 1.0 μL RNase H, 37°C/ 20 min. --> Store at -20°C


 * }