User:Xiling Shen/Notebook/Lab book

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Project Description/Abstract

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.	Digest (small) BglII/AlwNI RXN MIX FOR 1 SAMPLES B2Ms	4 EcoRI 	0.5 AlwN1	0.5 Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep" DNA:	pWW358 Keep:	1000 Reject:	2796 Image:GelExample.jpg .	Digest (small) BamHI/AlwNI RXN MIX FOR 1 SAMPLES B2Ms	4 BamHI	0.5 AlwNI	0.5 Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep" DNA:	pWW913 Keep:	3500 Reject:	931 Image:GelExample.jpg .	Digest (small) EcoRI/NheI RXN MIX FOR 1 SAMPLES B2Ms	4 EcoR1	0.5 NheI	0.5 Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep" DNA:	pWW935 Keep:	7462 Reject:	918 Image:GelExample.jpg .	Digest (small) EcoRI/XbaI/Bgl1 RXN MIX FOR 1 SAMPLES B2Ms	4 EcoRI	0.5 XbaI	0.5 Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep" DNA:	pWW914 Keep:	2453 Reject:	1134/914 Image:GelExample.jpg .	Digest (small) XbaI/PstI RXN MIX FOR 1 SAMPLES B2Ms	4 XbaI	0.5 PstI	0.5 Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep" DNA:	pWW916 Keep:	8274 Reject:	1190 Image:GelExample.jpg .	Digest (small) SpeI/PstI/AlwN1 RXN MIX FOR 1 SAMPLES B2Ms	4 SpeI	0.3 PstI	0.3 AlwN1	0.3 Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep" DNA:	pWW937 Keep:	4878 Reject:	2578,1350,427,263 Image:GelExample.jpg .