User:Jhdavis

I recently joined the Sauer Lab. clpX holla!!!!

Protocols

 * 1) Western Blots by Eyal Jhdavis::Western::Eyal
 * 2) GFP Purification by organic extraction Jhdavis::GFPOrganic
 * 3) Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
 * 4) Peptide cleavage Jhdavis::PeptideCleavage
 * 5) Flp recombinase Jhdavis::Flping
 * 6) ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
 * 7) Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
 * 8) regenerating qiagen columns Jhdavis::regenQiagen
 * 9) atpase notes Jhdavis::atpaseNotes
 * 10) Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip

Buffers

 * 1) PD Buffer
 * 2) ATP Regeneration Mix
 * 3) YEG-Cl
 * 4) Sauer:Davis Minimal Media
 * 5) Jhdavis::4x reducing SDS-PAGE loading buffer

Info

 * Jhdavis::GelFiltrationStandards
 * Jhdavis::MetCleavage
 * Jhdavis::lacIoperators
 * Jhdavis::DNA 1 letter codes
 * Jhdavis::Useful pymol
 * Jhdavis::A260:A280 DNA:Protein ratios
 * Jhdavis::fplc singup

Links

 * 1) Promoter standard
 * 2) novagen plasmid compatibility

Sheets

 * 1) [[Image:asdfsa.xlsx|Excel spreadsheet]]