IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/11/Rencheng Gao

11:00 Store the strains of and mini-prep: 1-11N+AraC(2), 1-5J+AraC(2), 1-1H+AraC(2), 1-2M+AraC(2), 1-5N+AraC(3), 1-2I+AraC(2) BioSpec(×50 μg/mL):

16:20 Double-enzyme digestion : 1-11N, 1-1H, 1-2M

22:30 DNA purification: fragments of 1-11N, 1-1H, 1-2M

00:30 Ligation Insert: fragments of 1-11N, 1-1H, 1-2M Vector: 1-23L