User:Mark X. Ling/Notebook/Micb 323/2009/03/10

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

SDS PAGE of Baculovirus Harvest

 * Amount of sample to be used in SDS page according to result from Bradford assay




 * we just used 15uL of samples into the sds page for recombinant as none of the protein assay had reliable result

Stimulated Macrophage [protein]
standard curve: Figure 1. BSA Standard Curve
 * [protein] in 350uL of lysate.
 * this is from the lowest protein concentration.

Table 1. BSA standard data. table 2. Macrophage lysate data.
 * BSA standard was incubated for 10minutes.
 * Data is from BSA Standards Eric M. and Sunny D prepared

total protein staining

 * heated at 92oC for 3 minutes

Post-lab

 * The Bradford for the stimulated Macrophage went well. Result were not as expected.
 * the effect of SDS were really shown in this lab when protein concentration were needed.


 * S9 Viability was not done


 * }