User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/17

{| width="800"
 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Lonely day
   1. Transform (again) both click beetle luciferases. I left the plates incubating at 37°C ON.
 * In the morning, I ran a gel to check the PCR that I left yesterday. It was too bad to notice that none of the colonies showed amplification.
 * What I decided to do was to:

2. Extract plasmid from several colonies during the next couple of days.  


 * }