SDS-PAGE

Running (lower) gel: 10 ml (2 MINIgels)
Chemical     8%     10%     12% Water        4.74   4.0     3.3  ml 30% AA(*)     2.66   3.3     4.0  ml LOWER buffer  2.5    2.5     2.5  ml 20% SDS       50     50      50   ul 10% APS       200    200     200  ul TEMED         8.2    8.2     8.2  ul

Stacking (upper) gel: 4 ml (2 MINIgels)
Chemical     4% Water        2.65 ml 30% AA(*)     0.67 ml UPPER buffer  0.65 ml 20% SDS       20 ul 10% APS       125 ul TEMED         6 ul

Buffers
LOWER buffer: 1M Tris base, pH 8.8 UPPER buffer: 1M Tris base, pH 6.8

Abbreviations
AA = acrylamide SDS = sodium dodecylsulfate APS = ammonium persulfate