IGEM:Peking University/2008/Experiment/PKU 0517 G2 1

Primer design

 * Primer 1
 * Primer name: F-Gal4-HindIII-Apal
 * Sequence(5'to3'):AAAAAGCTTGGGCCCTTTTAAGAGAGGACAGAGAAGCAAGCCTC
 * Tm:64.1°C
 * Primer 2
 * Primer name: R-Gal4-EcoRI-Xhol
 * Sequence(5'to3'):GGGGAATTCCTCGAGTGCGGGGTTTTTCAGTATCTACGATTCATT
 * Tm:64.0°C

PCR

 * conditions