Template:SBB10Project-28190

Carolyn Kwok

sbb32: O99 origin of replication
Source: pEC52 Target Sequence: agcgcctcagcgcgccgtagcatcgataaacattacgggatggggcgaaactgccacctgttcgaaatgacgcgcaaatgggcttacagggcgattcgtcagggctggcctgtattctcacaatggcttgatgccgtgatccagcgtgtcgaaatgtacaacgtatcgcttcccgttccgctttctccggcggaatgtcgggctattggcaagagcattgcgaaatacacgcacaggaacttcacgccggaaactttcgcacagtatgtggctgatacgcacacgccagaaattcaggctgcacgtggtcgcaagggcgggaaaattggaggtgctaagtctaagcgtggtgctgttgccacatctgcgcgaacgttgaaaccgtgggagactcttggaattagccgcgcctggtattaccaactgaaaaaacgaggtcttgtagagtagaccaaataaaacctatatcagataacagcgcttttttgagctgttggcgtttggt Vector: pBjk2741 Short description: oriO99 Genbank reference: D30060.1 Family: Origin of Replication

sbb24: O99 regulatory RNA
Source: pEC52 Target Sequence: acgattctgacgcattttttatgtttaccctggtgtttccttgtctttttgttttttttgcgtcagaaagcgactgagggtgttttaaggggtacgtacaacgggagttatggtaaatggatcgggttttcgggaaggttcgacaggatttgccgttgggtgtagtgtaagcgactgaaaaacaaacgcccctgaaattcgtgtctctccgccaagattgtcacgagaatacaggggcgaggtcggtcgataacctggaaggaatcgtaacacatgagcgccgcgcttcaatacttcgaagaaaatttaccccaccgtccctatcacacggatgatctcgcttttggtcttcgcatctccggcaaagggcgtgcgcttcttgcggggtacatccagcagaaccagcctcatgcgcagttctggctggtttttgacgt Vector: pBca9523 Short description: O99 5'UTR Genbank reference: D30060 (5…441) Family: Expressed RNA

sbb41: Constitutive RFP
Source: pBjh1601CK-Bjh1904 Vector: pBjk2741 Short description: {Ptet}.{rbs_RFP!} Family: Expressed CDS This part is used to constitutively (meaning all the time) express red fluorescent protein in E. coli.