User:Howard Boland/Notebook/Art from Synthetic Biology/2011/03/07

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Check Transformation
Few colonies (3-8)
 * 1) 1>6I, XL-1 BLUE, ok
 * 2) 1>12O, XL-1 BLUE, ok
 * 3) 1>22I, XL-1 BLUE-a, ok

Check Overnight Growth

 * 1) 1>6I, XL-1 BLUE, ok > 1.2 O.D. -> Move 1ml over to new bottle and grow to 0.5 O.D.
 * 2) 1>6I, XL-1 BLUE, ok > 1 O.D. -> Move 1ml over to new bottle and grow to 0.5 O.D.

Miniprep

 * 1) 1>6I, 1.5ml 0.5 O.D. -> elute in 50µl H20
 * 2) 1>6I, 1.5ml 0.5 O.D. -> elute in 50µl H20
 * 3) 1>6I, 5ml 0.5 O.D. -> elute in 50µl H20
 * 4) 1>6I, 5ml 0.5 O.D. -> elute in 50µl H20
 * 5) 1>6I, 6ml 1.2 O.D. -> elute in 50µl H20
 * 6) 1>6I, 6ml 1 O.D. -> elute in 50µl H20

Gel Plasmids

 * 1) 5µl 1kb ladder
 * 2) 5µl 1>6I, 1.5ml 0.5 O.D.
 * 3) 5µl 1>6I, 1.5ml 0.5 O.D.
 * 4) 5µl 1>6I, 5ml 0.5 O.D.
 * 5) 5µl 1>6I, 5ml 0.5 O.D.
 * 6) 5µl 1>6I, 6ml 1.2 O.D.
 * 7) 5µl 1>6I, 6ml 1 O.D.
 * 8) 30µ1>22I from Friday

Overnight Growth
3 bottles of 10ml culture inoculated from todays transformation and 10µl Ampicillin per bottle
 * 1) 1>12O, E0840
 * 2) 1>22I, I13504
 * 3) 1>6I, E0040

Transformation
Setup 5 transformations
 * 1) 1>12O, XL-1 BLUE (A.A.), ok
 * 2) 1>12O, XL-1 BLUE, ok
 * 3) 1>12O, DH5-a, blank
 * 4) 1>22I, DH5-a, blank
 * 5) 1>22I, XL-1 BLUE, blank


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