User:Fermenter User/Notebook/MACG F8R

{| width="800"
 * style="background-color: #cdde95;" align="center"|
 * style="background-color: #cdde95;" align="center"|




 * align="center" style="background-color: #e5edc8;" |


 * colspan="2" style="background-color: #F2F2F2;" align="right"|Customize your entry pages 
 * colspan="2"|
 * colspan="2"|
 * colspan="2"|

Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs Purple: Induction hrs

MACG F8R

Day -1 (May 19 Tue)
 pH sensor: monitor or control pH   dO2 sensor: monitor or control dO2 Condenser: Gas outlet OD probe: monitor cell growth ==> Not used! Replate it with septum Triple: Addition of media, Acid or Base if necessary MeOH sensor: Monitor EtOH (byproduct)  MeOH sensors also detects ethanol! :See reference #1 on the bottom of the page

                5/12/2009 (11:10)  Prepare small culture for inoculum (7.5mL)

       5/19/2009 (10:30)  pH calibration No Antifoam added 5/19/2009 (13:00) Pour media 5/19/2009 (13:30) Autoclave bioreactor 3 empty bottles/fermenter Base, Carbon source+Inoculation, Feeding 5/19/2009 (15:30) Cooling & Start Temp Control 5/19/2009 (15:45) Start F1 software 5/19/2009 (15:46) Start F2 software 5/19/2009 (15:47) Start F3 software 5/19/2009 (15:50) Connect MeOH sensor 5/19/2009 (16:00) Connect Base (400 ml) Prime the pumps Set pH                          5/19/2009 (16:30)  Wrap bioreactors with foil

                5/12/2009 (02:50)  Prepare small culture for inoculum (15mL)                 5/12/2009 (17:30)  Prepare small culture for inoculum (315mL)

Day 0 (May 20 Wed)
        5/20/2009 (07:10)  Add carbon source using pumps 5/20/2009 (07:20) Calibrate dO2 5/20/2009 (07:20) Set dO2

                5/20/2009 (08:00)  Fermentation 00:00 Inoculation 50 ml (F1 and F2) and 250 ml (F3) using pump.                 5/20/2009 (08:00) Fermentation 00:00 F1: OD (0.222) Contamination? (N) F2: OD (0.132) Contamination? (N) F3: OD (0.227) Contamination? (N)                 5/20/2009 (12:00) Fermentation 04:00 F1: OD (0.484) Contamination? (N) F2: OD (0.496) Contamination? (N) F3: OD (0.466) Contamination? (N)                 5/20/2009 (17:00) Fermentation 09:00 F1: OD (2.12) Contamination? (N) F2: OD (1.89) Contamination? (N) F3: OD (1.69) Contamination? (N)

Day 1 (May 21 Thu)
<Ann>                5/20/2009 (07:00) Fermentation 23:00 F1: OD (17.5) Contamination? (N) F2: OD (10.9) Contamination? (N) F3: OD (9.8) Contamination? (N) <Ann>                5/20/2009 (07:00-08:30) Fermentation 23:00-24:30 F1: Spin down cells and re-suspend with fresh media (1-L) Pour culture back into fermenter F2: Spin down cells and re-suspend with fresh media (1-L) Pour culture back into fermenter F3: Spin down cells and re-suspend with fresh media (1-L) Pour culture back into fermenter <Ann>                5/20/2009 (08:30) Fermentation 24:30 F1: OD (17.1) Contamination? (N) F2: OD (9.2) Contamination? (N) F3: OD (8.4) Contamination? (N) <Ann>                5/20/2009 (08:30) Fermentation 24:30  Induction 00:00 F1: Induce with Galactose (55 mL) F2: Induce with Galactose (55 mL) F3: Induce with Galactose (55 mL) <Ann>                5/20/2009 (12:30) Fermentation 28:30 F1: OD (22.5) Contamination? (N) F1: Induction 04:00 F2: OD (10.7) Contamination? (N) F2: Induction 04:00 F3: OD (9.5) Contamination? (N) F3: Induction 04:00 <Ann>                5/20/2009 (16:30) Fermentation 32:30 F1: OD (37.7) Contamination? (N) F1: Induction 08:00 F2: OD (13.4) Contamination? (N) F2: Induction 08:00 F3: OD (12.3) Contamination? (N) F3: Induction 08:00 <SungHye>            5/20/2009 (16:30) Fermentation 32:30 Harvest