IGEM:Peking University/2008/Notebook/Group 2/2008/07/17

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PKU_080717_G2_1

 * Screen for pGREF505-lexO clonies


 * Plasmid Miniprep-G4M1278-13~24
 * Sequencing-G4M1278-14/17/24


 * Result for last sequencing: G4M1278-1&2 are right point mutations.


 * Point mutation-T1278G/T1743G
 * Using right wtGAL4 TG-11


 * Primer Name:T1743G-F & T1743G-R


 * PCR system


 * conditions


 * Result: have about 7000 band, waiting for sequencing check


 * Digestion-point muation product-overnight/4°C


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