User:Anthony Salvagno/Notebook/Research/2009/10/30/Primer Melting Conditions

I want to figure out the Tm for my primers.

Tools
Manipulate a DNA Sequence mFold via Integrated DNA Technologies This is pretty easy to use. mFold via RPI DINAMelt This is what I am using for analysis here.

Approach
I have 4 oligos to compute the melting Temp (Tm) of. Into mFold I am inputting these parameters:
 * DNA sequence - there are two boxes. The left input is my oligo and the right input is the inverse compliment.
 * 50mM Na+
 * 2.5mM MG++
 * 10uM oligo concentration
 * Temp 37C

F853D
Tm = 68.2C

F834D
Tm = 67.2C

R2008
Tm = 66.2C

R1985
Tm = 69.6C