User:Karmella Haynes/Notebook/Polycomb project/2011/03/02

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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03/02/11

 * &#x2713; HEK luc-repression/ Pc-TF transfection: luc activity assay

Luc activity assay > Cells: dox-induced Gal4-EED expression/ 2 days, followed by transfection for 2 days 1-6. KAH160/MV1 (human Pc-TF), 0, 50, 100, 200, 400, 800 ng plasmid 7-12. KAH160/MV1 (human Pc-TF), 0, 50, 100, 200, 400, 800 ng plasmid

> Sample processing:
 * Discard 400 μL of the growth medium, resuspend cells in remaining ~600
 * Aliquot 3x 100 μL samples into 96-well plate
 * Save the rest for cell count (Millipore Scepter)
 * Normalize each value to cell count


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