User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/29

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Testing Lea primers

 * These were designed off A. ricciae sequence with the goal of amplifying all four copies of the gene

Reaction conditions

 * 95C 3min (60C 1 min, 95C 5s)x35

Gel
Blank! Tried the reaction a second time, and it also did not work, for A. vaga or A. ricciae

HCO LCO Screening
Load 1.0ul sample

Alien Gene LongPCR
Amplification of TEL_L_A : new primer screen

Evaporation in some wells - Re-Amp

Amplification of TEL_L_B

Load 1.0ul sample

Load 1.0ul sample