IGEM:Peking/2007/Switch-Notebook/2007-8-5

Experimenter: LXL

Precipitate and retract
SDY-EGFP^^(XhoI/SalI)

Ligation
SDY-EGFP   pLX007 SDY-EGFP   pLX003 pLX007     (self-control) pLX003     (self-control) SDY-EGFP   (self-control)

Colony PCR test of ligation product
Col.No. Product -- 1-46    T1T2-lacZa-pLX003 47,48   lacZa-pLX003(self-ligation-control) 49-76   lacZa-pLX007 78,79   pLX007(self-ligation-control)

8 are incubated.