User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/10/06

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Wash
 * Wash all 3 sets 3 x 15' in PBST
 * Secondary Antibody (1:500 in 2% NGS)
 * Tdt-Myb anti-mRFP incubate with anti-rabbit 488 O/N at 4°C
 * GFP-Myb anti-BAM incubate with anti-mouse 633 O/N at 4°C
 * GFP-Myb anti-SA incubate with anti-guniea pig 633 O/N at 4°C


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