TBS

Purpose

 * TBS stands for tris-buffered saline and is used in many protocols for washing

Recipe

 * Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl


 * Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again


 * TBST: For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.
 * Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
 * Usual concentration of Triton X-100 in TBST is 0.1%.