Wittrup: Yeast Minimal Media

SD-CAA, SG-CAA, SD-SCAA, SG-SCAA

Liquid, 1000 mL Add ddH2O to final volume. Filter sterilize.
 * Carbon source
 * SD: 20g Dextrose (D-glucose)
 * SG: 20g Galactose
 * Nitrogen source
 * 6.7g Yeast N2 base
 * Amino acid source
 * 5.0g CAA (-ADE, -URA, -TRP)
 * Or, Synthetic CAA..
 * Buffer
 * pH 6.0 phosphate buffer (standard)
 * 10.19g Na2HPO4.7H2O (which equals 5.40g Na2HPO4 anhydrous)
 * 8.56g NaH2PO4.H2O
 * pH 4.5 citrate buffer (optional: lower pH discourages bacterial growth)
 * 10.4g sodium citrate
 * 7.4g citric acid monohydrate

Plates, 1000 mL
 * Filter Sterilize:
 * Carbon source, Nitrogen source, Amino acid source in 0.1*(Final Volume)
 * Autoclave:
 * Buffer salts, 15-18g agar, 182g sorbitol in 0.9*(Final Volume)
 * Add first mixture to the autoclaved mixture after it has cooled to below 50 oC (touchable by bare hand)