Talk:Synthetic Biology:BioBricks/Standard FACS protocol


 * RS 12:35, 23 March 2006 (EST): I posted Barry's standard protocols from ages ago for Pete Carr. This is severely in need of an update.


 * 1) Use defined rich media with glycerol as the carbon source wherever possible (see Neidhardt_EZ_Rich_Defined).
 * 2) Use beads to calibrate runs against one another and control for machine variation and PMT settings etc.
 * 3) If possible use MG1655 as the background strain because at least its sequence is known in contrast to a lot of other strains whose exact genotype can be either fuzzy or wrong.
 * 4) Move to using a near single copy plasmid for all characterization. See Synthetic_Biology:Vectors/Single_copy_plasmid.