Matt Gethers/CRI, Thailand/Labwork/Polyacrylamide Gels/Week of 7.7.08

=Week of 7.7.08=

7.8.08

Poured gel and prepared samples according to protocol. My samples were 1 ml of the 200 ml culture used for isolation of HmgR before induction and 1 ml of the same culture after induction. Because I had only two samples, I loaded, 5, 10, and 20 &mu;l of each.

Gel looks good - protein appears in induced lanes at the correct location (~28 kDa) and not in uninduced lanes. Presence also increases with concentration.