User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/25

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=WetLab: LovTap + promoters=

Transformation report
The transformation from 240810 got well, for all the promoters a considerable amount of colonies were gotten.

Transformation
The ligations from 240810 were transformed into DH5α competent cells following the next protocol. These have the LovTap product from the colony number_9.
 * 20' on ice
 * 1' @ 42ºC
 * 5' on ice


 * 1h 1mL of LB
 * 1' @ 3000rmp

As a positive control was used the plasmid N.19[Am]


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