Template:SBB10Project-19395

Jeni Lee

sbb34: Ala6 linker
Source: Synthetic Target Sequence: AAAAAA (protein) Vector: pBjk2741 Short description: Ala6 linker Flavor: { } Family: Linker This part encodes a peptide linker. So, the peptide sequence is AAAAAA, but you'll need to generate a DNA sequence that encodes this peptide. Your peptide will be used as a spacer element to separate the dual tags that we'll use to quantify protein expression levels.

sbb07: Tn5 Transposase
Source: pRL27 Target Sequence: MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKSITISSEGSKAAQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQSKLDGFLAAKDLMAQGIKI* Vector: pBjk2741 Short description: Flavor: { } Family: Transposase