User:Tara K. Luckau/Notebook/Team ConGen/2011/03/04

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Scun22 Optimization PCR Gel

 * from 23 february 2011 PCR (full temperature and buffer gradient)

Gel Specs

 * Pour: 270mL 1xTAE +  5g agarose (supposed to have been 5.4g)  +  27µL GelRed
 * Run: 40 min @ 110V

Gel Images

 * [[Image:20110304_Row1.TIF|750 px]]
 * [[Image:20110304_Row2.TIF|750 px]]
 * [[Image:20110304_Row3.TIF|750 px]]


 * Only Buffer G yielded any product ... zoom in:
 * [[Image:20110304a_Row3Right.jpg|750 px]]

Conclusions

 * doesn't look like a typical MgCl2 and temperature gradient PCR
 * inconsistent banding patterns across temperatures with dropouts


 * regardless, there is mispriming in all conditions that yielded any product
 * Scun22 might not be a primer that will work for Sceloporus occidentalis