IGEM:Brown/2007/Lab Protocols/Quick Tform into E. Coli from Qiagen prep DNA -from Brodsky

Quick Tform into E Coli from Qiagen prep DNA


 * 1) Thaw competent Cells
 * 2) Add 1uL plamid and 5uL competent Cells
 * 3) sit on ice 10-30 mins
 * 4) take up cells in 100uL LB
 * 5) plate on LB/AMP plates
 * 6) incubate at 37*C.