User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/05

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis
To D:
 * anti-PCNA, anti-BAM stained GFP-Myb, RFP-H2av
 * Wash 3 x 15' @ RT in PBS-T
 * tdt-Myb
 * Stain 30' @ RT with oliGreen (1:500) and RNAse A(1:10) in 2% NGS
 * Wash 4 x 5' in PBS-T
 * 9 Day
 * Stain 30' @ RT with Topro3 (1:1000) in 2% NGS
 * Wash 4 x 5' in PBS-T
 * All
 * Mount in Vectashield


 * }