Template:SBB-Protocols Zymo2

Small-Frag Zymo Cleanup
The following procedure removes the polymerase, dNTPs, buffer, and most of the oligonucleotides from a PCR reaction for fragments smaller than 300bp. It also will remove the buffer and restriction enzymes from a restriction digest reaction.


 * 1) Add 100 uL of Zymo ADB buffer (brown bottle) to the reaction.
 * 2) Transfer into the Zymo column (small clear guys)
 * 3) Add 500uL of Ethanol and pipette up and down to mix
 * 4) spin through (15s), discard waste.
 * 5) Add 200 uL of A4 Wash Buffer (which is basically 70% ethanol)
 * 6) spin through (15s), discard waste.
 * 7) Add 200 uL of A4 Wash Buffer
 * 8) spin through, discard waste.
 * 9) spin for 90 seconds, full speed to dry.
 * 10) elute with water (spin 60s) into a fresh Eppendorf tube