IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/09/21

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Miniprep O/N cultures C,D,G,H

 * Protocol: Rafael's Alkaline Lysis Protocol
 * Resuspend DNA in 70uL of water
 * Nanodrop:
 * [C] = 1777.0ng/uL
 * [D] = 1318.5ng/uL
 * [G] = 1373.8ng/uL
 * [H] = 842.5ng/uL

Restriction Digest

 * Protocol: "digest" protocol from biobrick


 * x = amount of DNA
 * DNA:
 * Cut terminator (suffix) at E/X
 * Cut D and G at E/S
 * Add 1uL of each appropriate enzyme

Digest for 2 hours; start at 3:20pm

Gel verification colony PCR products
Gel orientation: Results:
 * Protocol: gel verification protocol in Protocol (SOP)
 * Machine conditions: 0.5x TBE buffer, 100V, 60min


 * Digestion failed.

Re-Restriction Digest

 * Protocol: "digest" protocol from biobrick


 * DNA:
 * Cut terminator (suffix) at E/X
 * Cut D and G at E/S
 * Add 1uL of each appropriate enzyme

O/N digestion starting at 9:30pm


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