BCH080306-Construction of pSB1A2-J23041

[pK2 (presumably on pSB1A2)]+[J23008] Digest pJ23006-J23008 (XbaI/AlwnI, neb2, small-648bp) Paste into pSB1A2-J01007 (SpeI/AlwnI, neb2, large-1593bp) Product is pSB1A2-J23041