User talk:Allen Lin

20.109 Material
MOD2:

Purpose: To design a primer to amplify a TAP TRP1 fusion gene. After PCR, the fusion gene will have additional elements, i.e. homologous regions of the S. cerivisiae gene YGR097W flanking the TAP TRP1 gene, to enable C-terminus modification of YGR097W via homologous recombination.

Forward Primer 5’ AAGGTTAATTTTGACATCGAGGAAGAGCAAGAAGGACAA TCCATGGAAAAGAGAAGATG

Reverse Primer 5’ AGATTTATCTGATATGCTCAATTTCCCCTCCCATTTTCA TACGACTCACTATAGGGCGA

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