Lissa1:EZ Yeast Transformations

Preparation of Competent Cells
 * 1) Pellet the cells at 500g (setting 3ish on centrifuge) for 4 minutes.
 * 2) Discard supernatant.
 * 3) Add 10 mL EZ 1 Solution to wash. Repellet and discard supernatant.
 * 4) REsuspend in 1 mL EZ 2 Solution
 * 5) SLOWLY freeze (in styrofoam container) in -80 freezer.

Transformation
 * 1) Mix 50 uL competent cells with .2-1 ug DNA, add 500 uL EZ 3 solution and mix.
 * 2) Incubate at 30 C for 45 minutes. Mix by flicking every 15 mins or so.
 * 3) Spread 50-150 uL of transformation on your plate.
 * 4) Incubate at 30C for 2-4 days to allow for growth of transformants.