User:Tara K. Luckau/Notebook/Team ConGen/2011/06/22

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SCOC Multiplex 1

 * SCOC multiplex diversity panel with labelled primers to send to frag! Woot!

PCR

 * [[Image:20110622 PCR.jpg|800 px]]

Gel

 * used left-over gel from 13 June 2011


 * see 13 June 2011 for banding pattern expectations
 * [[Image:20110622 Gel.jpg|600 px]]
 * a whole lotta nada :(
 * very strong primer band indicates no amplification took place
 * might be a combination of:
 * having to make primer dilutions last-second
 * pipetting 0.8µL DNA
 * using the flat-top-no-skirt PCR plate that didn't fit into the thermal cycler (and thus doing full-reaction transfer to cycler-friendly PCR plate)
 * therefore taking a long time for PCR set-up

Next Step

 * re-do PCR, but have 8-well strip with 0.8*4 µL DNA plus Big MM, then transfer from Mini MM straight across into PCR plate
 * might also be worth doing a PCR similar to that from 13 June 2011 - uniplex of each primer pair, plus multiplex - to 'test' labeled primers
 * over night, test full-skirt PCR plate with flat-top 8-well strips to see how much evaporation occurs - this is how I plan to ship for frag
 * shit, now no matter how soon I can ship, it won't get to UAGC till Monday


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