IGEM:MIT/2007/Notebook/2007-8-4

Double Digests!!!
5.3 µl    I14032 ( 188.4 ng/µl) 0.5 µl    BSA 5 µl      NEB2 Buffer 1 µl      SpeI 1 µl      PstI 37.7 µl     H20 -- 50 µl     Total

42.5 µl   R0051 (16.8 ng/µl) 0.5 µl     BSA 5 µl        NEB2 Buffer 1 µl        SpeI 1 µl        PstI -- 50 µl      Total

12 µl      B0034 (84.9 ng/µl) 0.5 µl     BSA 5 µl        NEB3 Buffer 1 µl        XbaI 1 µl        PstI 30.5 µl   H2O -- 50 µl      Total

5 µl         CPX (1µg/5µl) 0.5 µl      BSA 5 µl         EcoRI Buffer 1 µl         EcoRI 1 µl         SpeI 37.5 µl    H2O -- 50 µl       Total

16 µl      B0014 (62.8 ng/µl) 0.5 µl     BSA 5 µl        NEB2 Buffer 1 µl        XbaI 1 µl        EcoRI 26.5 µl   H2O -- 50 µl      Total