User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/15

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Rotifer DNA extraction

 * add 1ml of 1M NaCl to a 6 well dish filled with rotifers, they curl up
 * each well is about 10ml, resulting in a 100mM NaCl solution
 * spin at 1000g for 5 minutes
 * put into 1.5 ml tube, spin again
 * wash with small amount of water and go to extraction kit (quiagen micro)
 * yield 7ug for vaga and 5ug for ricciae