IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/07/04

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ABSTRACT

 * Ligation of pBBRMCS5 plasmid and preffix-RFP-suffix fragment both previously digested with ApaI and SacI.

Paulina and I did 3 ligation reactions with the following proportions:

Reaction 1: It has more PCR product than vector.

Reaction 2: It has less PCR product than vector. We did this reaction because the PCR product was more concentrated than the vector so maybe with the previous reaction there is the possibility that the PCR products ligate with each other and not with the vector.

Reaction 3: This is a ligation control without PCR product.

We left this reactions incubating at 16°C overnight.


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