IGEM:IMPERIAL/2007/Notebook/2007-8-2

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/2 view=threemonths format=%name/%year-%month-%day weekstart=7

Preparation of Stock Solutions

 * 1x 1 litre of 1M Tris-Cl
 * 1x 500 ml of 50x Tris-acetate

Preparation of Competent Cells

 * 1) Picked colonies from agar plates
 * 2) Grew colonies in LB media
 * 3) *DH5Alpha
 * 4) *XL1Blue
 * 5) Incubated cultures at 37°C overnight