840:153g:Projects/project4/2009/03/31

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] MoldBusters' Journal
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Tuesday 3/31
Josh and Casy
 * New primers arrived which will reduce hairpin formation.
 * Diluted primers to 100μM and 10μM concentrations. Stored in the freezer.
 * Prepared PCR to amplify the aprE gene. The PCR was ran with 1.0μL and 0.1μL of DNA at temperatures from 45°C to 60°C. A positive and negative control were also prepared.
 * Thursday, a gel will be ran with the PCR product to determine if the aprE gene was amplified.

Derek and Katy
 * Ran a gel electrophorisis on our PCR amplification of wintergreen.
 * The DNA samples 7 and 8 showed up with several bands on them.
 * DNA sample 3 did not show up at all so we wont be using that one again.

Oggie
 * Ran a gel of my nprE PCR amplification using 3 different forward primers
 * All results looked the same regardless of forward primer used
 * PCR failed so I setup several tests to determine the cause of failure


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