IGEM:IMPERIAL/2007/Projects/Hrp System/Testing/Protocol Hrp

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Equipment

 * Twinkle Plate Reader Fluorometer
 * 96 well plate
 * Eppendorf Tubes
 * Gilson Pippettes
 * Plastic Curvetts
 * Incubator 37o shaker
 * Vortex and Centrifuge
 * Spectrometer

Materials and Reagents

 * M9 minimal media 50μg/ml Ampicillin
 * Inducer of promoter at varying concentrations
 * Recombinant acGFP of known concentration
 * Agar Plates
 * E.coli strain containing vector

Promoter Test Construct
The promoter pLux is to be tested at 8 varying ranges of inducer concentration.
 * pLux will have a range of 0 to 100μM AHL added at intervals of 12.5μM  Protocol

Device Test Construct
For this test construct we initially need to run a preliminary test in order to find out the time intervals and input ranges we need. We will start the preliminary test using the range of concentrations used initially for promoters.
 * Preliminary experiment Protocol
 * Device experiment Protocol