SR

Summary
Super Rich Medium (aka SR Medium). This medium is optimized for high-yield protein expression in Escherichia coli.

Ingredients
Solution A
 * 1) 20 g tryptone
 * 2) 20 g yeast extract
 * 3) 10 g beef extract
 * 4) 11 g glycerol
 * 5) 900 mL H2O

Solution B
 * 1) 2.32 g KH2PO4
 * 2) 16.4 g K2HPO4*H2O
 * 3) 100 mL H2O

Protocol

 * 1) Dissolve ingredients of both solutions.
 * 2) Pour into flasks or bottles (Solution A in 2 L flask or greater, Solution B in 250 mL flask or greater).
 * 3) Autoclave (liquid cycle)

Source
Feil, I. (1992) PH.D. Thesis, Technische Universtiaet Carolo-Wilhelmina zu Braunschweig, Braunschweig, Germany