User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/07/29

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Thursday 29th July 2010
Today I digested the pBluescript II KS + plasmid and the cI inverter PCR with EcoRI-HF and PstI for posterior cloning of the inverter into the plasmid as follows:

Reactive..............1x [ul]

DNA....................20

BSA.......................1

Buffer2..................4

H2O....................11

EcoRI-HF...............2

PstI........................2

Total....................40

For the plasmid I did it twice.

Then I checked the DNA quantities in an agarose gel for posterior ligation.



1º ladder

2º pBluescript II KS + plasmid

3º pBluescript II KS + cut with EcoRI-HF and PstI

4º pBluescript II KS + cut with EcoRI-HF and PstI

5º cI inverter PCR cut with EcoRI-HF and PstI


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