User:Anthony Salvagno/Notebook/Research/2009/12/08/Back in the Habit: Getting Reacquainted with Lab

Now that I am back from NY (which was a great trip), I need to get on the horse and get things done. I have until the middle of Feb to try and get preliminary unzipping of Shotgun Clones. I have to start with preliminary unzipping of tetherable pBR322. I need a list of things that I can work on imminently, and a meeting with Koch. Also I will have to do some work on the tweezers
 * 1) Meet with Koch to discuss list
 * 2) Tether pBR322 fragments
 * 3) Make new anti-dig aliqots (probably need to buy more soon)
 * 4) Good sonication of beads
 * 5) Go to Osley Lab and get shotgun fragments
 * 6) SapCap shotgun fragments
 * 7) Ligate shotgun fragments to tethering construct
 * 8) Tether the bitches
 * 1) Get familiar with tweezers
 * 2) measure beam width for AOM
 * 3) *Anthony Salvagno 18:02, 8 December 2009 (EST):The beam diameter is 1mm
 * 4) try isolator
 * 5) *Anthony Salvagno 17:26, 8 December 2009 (EST):We tried this and ended up burning the PBS on the isolator. Guess this isn't an option.
 * 6) practice unzipping and try for crappy data with jumpy beam
 * 7) *Brian is making stretching DNA tethers from his 1.1kb PCR so we will try stretching experiments. I will need Koch to show me how to analyze after trying to unzip.  I will also have to relearn how to unzip.