User:Karmella Haynes/Notebook/BioBrick cloning/Entry Base

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mm/dd/yy

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Minipreps > Check with E/P digests

Assemblies
 * 1) BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size
 * 2) BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size

> Digests (Fermentas FD) --> Specific notes

> Measure conc.'s

> Dephosphorylation (Roche)

> Ligations

Oligo annealing
 * 1) New BB 1
 * 2) New BB 2


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