IGEM:MIT/2007/Notebook/2007-7-26

Agenda

 * 1) Western Blot of AHL-induced CPX cells
 * 2) Transform the second mer-gfp-1AT3 ligation
 * 3) Pick and LC last night's plated transformations (mer+gfp+1AT3)
 * 4) Streak Barry's BL21 competent cells
 * 5) Make glycerol stock of BL21 trans. with F+B+CPX and pICCSP

Results of First Plating (yesterday's mer+gfp+1AT3 transformation)

 * All plates were spread with 150µL of transformants

Plate         # Colonies      Notes 1µL DNA       0 5µL DNA       8 10µL DNA      0 (-) control   4 pUC18 on LB   smear pUC18 on Amp+ lawn

Results of Second Plating (yesterday's mer+gfp+1AT3 transformation)

 * All plates were spread with remainder of transformants

Plate         # Colonies      Notes 1µL DNA       20              smear with some tiny distinct colonies 5µL DNA       21 10µL DNA      12 (-) control   0 pUC18 on LB   smear pUC18 on Amp+ 2880