Berglund:Protein Buffers

2X SDS loading dye

 * 1 mL 1M Tris-HCl pH 6.8 (fc=50mM)
 * 42 mL 10% SDS (fc=2%)
 * 1 mg Bromophenol Blue (fc=0.1%)
 * 2 mL 100% glycerol (fc=10%)
 * 1 mL 2M DTT (fc=100mM)

5x SDS loading dye (by Julien)

 * 12.5 mL of 1M Tris pH 6.8         (f.c.=50mM)
 * 100 mg of Bromophenol Blue    (f.c.=0.2%)
 * 25 mL   of 100% Glycerol          (f.c.=10%)
 * 3.85 g   of DTT                          (f.c.=100mM)
 * 5 g        of SDS                          (f.c.=2%)
 * H2O up to ~50 mL

Note: f.c. values are the final 1X concentrations (NOT the 5X concentrations).