IGEM:MIT/2007/Notebook/2007-7-31

=Agenda=
 * 1) Run mercury assay
 * 2) Redo live immunostaining with altered protocol
 * 3) Enter working parts into BioBricks Registry
 * 4) Find constitutive promoter and perform 3A assembly with a RBS and CPX
 * 5) Redo transformation of BL21 w/ mer.I13500 in 1AT3

CPX Assay
Varied cell concentrations: (OD)
 * .1
 * .3
 * .5
 * 1

CPX Immunostaining

 * Induced cells at 0.1 OD, incubate for 1 hour
 * 10 samples total: 2 each for 0(- control), 10^-8, 10^-7, 10^-6, 10^-5 concentration AHL
 * Changed secondary Goat Anti-Mouse antibody concentration to (1:200)

Transformation of mer.I13500 in BL21

 * Nothing grew on any of the plates (not even positive control with pUC18)
 * Perhaps cells not competent?
 * We should redo tomorrow