Prather:Protocols

Prather Lab Protocols

 * [[media:New_Member_Guide_-_6June11.pdf‎|Prather Lab New Student Guide]] (PDF, Updated June 2011)
 * [[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (PDF, Updated 15Mar2007)
 * [[media:Prather_Lab_Experimental_Protocols_25Jan09.pdf|Basic Molecular Biology Protocols]] (PDF, Updated Jan. 2009)
 * Growing E. coli in liquid culture and on solid media
 * DNA electrophoresis
 * Gel extraction
 * PCR
 * DNA digestion with restriction enzymes
 * DNA ligation reaction
 * Electrocompetent transformations
 * Preparation of freezer stocks


 * [[media:Advanced_Protocols_2009.pdf|Advanced Molecular Biology Protocols]] (PDF, Updated Jan. 2009)
 * Preparation of bacterial lysates by freeze-thaw
 * Bradford total protein assay
 * Boiling-lysis preparation of pDNA
 * Sample dialysis
 * Running a protein gel


 * Gibson Chew Back and Anneal Isothermal Assembly
 * [[media:P_putida_protocols.pdf|P. putida Protocols]] (PDF, Updated Feb. 2009)

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