SIL072006-Construction of pSB1A2-J23015

F plasmid with OriT knockout PCR FORlamF/ForlamR on pKD3  (~1000bp, dPN1, Neb2) Electroporate into pKD46xpOX38 Product is pSB1A2-J23015 - ForlamF Forward oligo to knockout OriT: tttatccgtaaataatttaacccactccacaaaaaggctcaacag gtgtaggctggagctgcttc ForlamR Reverse oligo to knockout OriT: ggaccatggctaattcccat ctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaa