IGEM:MIT/2005/Techniques

Lab Protocols

 * ../Resuspend Primers To Working Concentration/
 * ../Pipetman use, working w/ bacteria, PCR/
 * ../Miniprepping DNA, Agarose gels, RE digests/
 * ../Excising DNA from gels, purifying DNA from gels/
 * ../Ligations, Transformations/

Others

 * Codon Usage
 * Translate Tool
 * Orf (open reading frame) Finder
 * Amino Acid Codes
 * ../Ray's Tools/
 * Restriction Sites: http://www.bioscience.org/urllists/restrict.htm
 * EcoR1
 * G AATTC
 * CTTAA G
 * HaeIII
 * GG CC
 * CC GG
 * BamH1
 * G GATCC
 * CCTAG G
 * Pst1
 * CTGCA G
 * G ACGTC
 * EcoKI
 * 5'AAC(N)6GTGC3'
 * EcoBI
 * 5'TCA(N)8TGCT3'
 * Not1
 * 5' GCGGCCGC 3'
 * Xho1
 * 5' C TCGAG 3'


 * Base pair finder - read the readme first... if you have any questions, ask me..
 * http://web.mit.edu/ymk/Public/igem/README.txt
 * http://web.mit.edu/ymk/Public/igem/sequen.sh
 * http://web.mit.edu/ymk/Public/igem/ByeNewLine.class
 * Epitope Tagging
 * QuantumDot for detection/illuminescence