IGEM:MIT/2007/Notebook/2007-7-4

Agenda

 * mini-prep pSB3k3 from DB3.1 from incubation yesterday
 * streak some DB3.1 for future use
 * Pick colonies, incubate liquid cultures of DH(alpha)5 - to be taken out of hot room at 4am tonight

Took out DB3.1 liquid cultures

 * Yay, all five tubes have growth
 * Took from all five cultures for miniprep
 * Will streak from cultures 1, 2, 3

Mini-Prep - Nanodrop of purified pSB3k3
1. 8.4 ng/µL 2. 13.1 ng/µL 3. 6.5 ng/µL 4. 11.2 ng/µL 5. 8.1 ng/µL

Streaked DB3.1

 * DB3.1 is the strain that is resistant to the ccdB toxin (
 * Streaked three plates
 * Left in 37C room to incubate overnight

Incubated liquid cultures of DH5a

 * Two 10 mL liquid cultures of singly colony DH5a (picked from last night's incubated plates) were put into the 37C room to grow overnight