IGEM:UNAM-Genomics Mexico/2009/Notebook/Wiki Creation/2011/06/14

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Objectives

 * Design a method for converting proyect text into HTML suitable text.
 * Design in silico the four intergenic operon parts for Gibson Assembly with the Silver_lab ORF's, to be synthesised.

Week meeting
During this meeting we agreed that only Abiel and I will edit the page unless an exceptional situation with immediate editing need is encountered. Since we had already agreed in an notebook like displaying of our page, I agreed in presenting a notebook layout for our wiki.

Our team still has no logo. We agreed in presenting logos for the last time on friday 17. I am presenting one alternative logo.

As part of our experimental procedures we optimized the ORF's of our system according to Rhizobium etli 's Codon usage Adaptation Index CAI. We asked Pamela Silver's Lab for the un-optimized ORF's used in our system to reassemble it without the optimization as a control experiment. However, in order to assemble them in our designed operons through Gibson Assembly we need some complementarity at the end of our sequences. We will send for synthesis the intergenic spacers and the promoters of our system with 3bp ORF overlapping regions (non-optimized). I will work on this as a high priority task, along with Helena and Daniel.

Achievements

 * Intergenic parts design is done.
 * The method design was NOT achieved.