SBB09 Construct44

Construction of Polystyrene-Binding Peptide

Wobble Ons003F/Ons004R          (67bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5   (EcoRI/BamHI, 3171+910, L) Product is pBca9495AK-M10021     {} Ons003F  Forward construction of Poly_bind basic part     ccataGAATTCatgAGATCTttcaaattctggctatacgaacatgtaatacggggg Ons004R  Reverse construction of Poly_bind basic part     ctgatGGATCCcccccgtattacatgttcgt

JCA: Your construction file is "correct", but in general repeats on the 3' end of an oligo are bad news, particularly if they are G's since 4G's makes a particular secondary structure in and of itself. See revision:

Construction of Polystyrene-Binding Peptide

Wobble Ons003F/Ons004R          (67bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5   (EcoRI/BamHI, 3171+910, L) Product is pBca9495AK-M10021     {} Ons003F  Forward construction of Poly_bind basic part     ccataGAATTCatgAGATCTttcaaattctggctatacgaacatg Ons004R  Reverse construction of Poly_bind basic part     ctgatGGATCCcccccgtattacatgttcgtatagccagaat