User:Alexander S Mikheyev/Notebook/P. rapida partial genome/2009/11/13

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] P.rapida genome
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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To Do

 * add +A onto end-repaired sample
 * set up overnight ligation

+A addition

 * end-repaired sample with Invitrogen PureLink PCR Purification Kit, eluting in 35 μl of buffer
 * 17ng/ul, or about 4 pmol total DNA @ 200bp


 * Incubate for 30 minutes at 37°C.
 * MinElute PCR Purification Kit to purify on one QIAquick MinElute column, eluting in 10 μl of EB.

Adapter ligation

 * 16C for overnight