User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/15

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Overnight Growth check
All bottles grew (Kanamycin backbone slowest)
 * pSB1A3
 * pSB1C3
 * pSB1K3
 * pSB1T3

Prepare media
Prepared R/L-media (not working)

Setup Digestion to check reagents
I setup an EcoRI/PstI on J04450

Run digestion on Gel
The sample was run on gel after 2hours digestion in 37ºC

Gel Result
1% Agarose Gel, 15-FEBRURARY-2011-17:44 GMT
 * 1) Lane 1: 10µL  1kbp NEB Quick Ladder
 * 2) Lane 2: BLANK
 * 3) Lane 3: 5µl  pSB1A10/BBa_J04450/A1  EcoRI|PstI
 * 4) Lane 4-8: BLANK



A comparative gel can be found in the Partsregistry gel (Lane 1/ Lane M is a 2-log NEB ladder) 2log NEB ladder

FSM inoculation
I corrected my inoculation from yesterday by fixing the amount of carbon source.Yesterday I made a wrong calculation and added x100 less than needed. The samples (5x10ml) are growing in 30ºC and I have one sample 200ml growing in 28ºC also containing ferric citrate.

M9 Media with Indole deficient E.coli
I setup a 10ml tube with growth of this strain


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