IGEM:metu/2009/Notebook/wound dressing/2009/10/15

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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15.10.2009
1. To obtain large amounts of protein (EGF), again we prepared LB-Broth.

250 ml LB+ Lard 1+ABC
 * This substance was put into shaker, 37 °C and 190 rpm. (Overnight incubation - 16 hours)

2. Collagen sponge wells were taken.


 * Keep the wells at -80 C at 24 hour.


 * Lyophilize the frozen solution.

3. Starting to preparation of gelatin sponge.