User:Andy Maloney/Kinesin & Microtubule Page/W-PEM

Recipe

 * PEM
 * 1.0mg/mL whole casein.
 * I will typically prepare a total volume of 25 mL. This means that I will weigh out 25 mg of whole casein to add to the 25 mL of PEM.

Procedure
When I prepare the whole casein solution, I use a condenser since I will be heating up the solution. The condenser allows me to heat up the solution without having to worry about evaporation. I've used the condenser for ion exchange before which requires you to boil things for several hours. These things work quite well as I was able to boil my column media without having to watch it the entire time in order to add water back to the solution.

Using the condenser is pretty straight forward. Here are the steps I take to set it up properly. Do not plug in the pump until the step to turn it on comes up! I have found that the casein solution should last for up to 6 months.