User:Fermenter User/Notebook/BROM H1

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM H1 (F6)

Day -1 (Feb 2 Mon):
           2/2/2009 (15:00) Bring media to fermenter room           2/2/2009 (15:00) Assemble bioreactor 2/2/2009 (15:20) Add 10 ml of Antifoam. 2/2/2009 (15:30) Start Sterilization (Liq 45 min) 2/2/2009 (16:00) Cooling of bioreactor (Temp setup) 1 L/min air, 250 rpm

Day 0 (Feb 3 Tue):
Sung-Hye: CHBE Class Auditing 9:30 - 11:00

2/3/2009 (8:25) Start Fermentation Software for Fermenter #6 2/3/2009 (8:30) Connect Acid (500 ml) and Base (400 ml), prime pumps Start pH control 2/3/2009 (8:30) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 2/3/2009 (8:30) Set dO2 2/3/2009 (8:30) Completion of bioreactor set up: Ready for inoculation -                 2/3/2009 (9:30) Inoculation: inoculation volume (+ 600 ml) Fermentation 0:00 Increase rpm to 400            2/3/2009 (9:30) Sampling  Fermentation 0:00 Cell weight ( mg), Activity ( %, x100), Activity ( %, x1000)           2/3/2009 (16:00) Increase rpm to 600            2/3/2009 (00:00) Sampling  Fermentation 0:00 Cell weight ( mg), Activity (%, x100), Activity ( %, x1000)

Day 1 (Feb 4 Wed):
5 am - 9:15 am : O2 tanks ran out            2/4/2009 (9:15) Fermentation 23:45 Open O2 tank Acid (500 ml) Base (170 ml)             2/4/2009 (9:30) Sampling  Fermentation 24:00 Cell weight ( mg), Activity ( %, x100), Activity ( %, x1000)             2/4/2009 (00:00) Sampling  Fermentation 00:00 Cell weight ( mg), Activity ( %, x100), Activity ( %, x1000)

Day 2 (Feb 5 Thu):
            2/5/2009 (9:30) Sampling  Fermentation 36:00 Cell weight ( mg), Activity ( %, x100), Activity ( %, x1000)            2/5/2009 (11:30) Fermentation 00:00 Harvest Acid (390 ml) Base (100 ml)


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