IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/15

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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July 15th, 2010

 * Prepared E. coli with copper solutions in wider flasks for better oxygenation
 * Retested copper promoter constructs made from overnight cultures the night before and incubated at 37C for 4 hours
 * Aliquoted 1ml of the copper sulfate treated cultures to microcentrifuge tubes, centrifuged, and observed pellets for fluorescence with UV lamp
 * Compared optical density readings of the Copper sulfate treated cultures using various available spectrophotometers (Perkin Elmer Lambda 20, Thermo H, plate reader)
 * Rechecked RBS efficiencies of fluorescent proteins using RBS calculator
 * Discussed project description alterations
 * Discussed different wiki’s and own ideas for the site
 * Made more liquid cultures of E. Coli
 * Worked on web page design for the wiki
 * Investigated approaches to eliminate copper from media: Chelex, EDTA, M9 Minimal media


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