User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/08/08

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Maize Endophyte Biofertilizers
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The Daily Grind

 * Well, I sectioned my arabidopsis grown under short day conditions. No mutant phenotype. Plants were big and healthy (well watered, not fertilized, with constant wind and 80 umol of light). I know the variation originally was some sort of stress, but probably not light. I will now try to plant a bunch of arabidopsis Atctl2 SAIL mutants in different stress conditions to see if I can get that damn phenotype. Will use Sunshine mix LA4 soil with no fertilizer (make double here so you can withhold water for some at the bolting stage), 15-5-15 at 200 ppm, or 15-5-15 at 200 ppm plus 50 mM NaCl mixed into the soil at the start. Will put them in 24 hour light 100 umol light, with a fan to blow them around.
 * Will try again to make a double mutant for AtCTL1 and AtCTL2, using Atctl1 as the receptor.
 * Making overnights of GFP tagged endophytes for glycerol stock
 * Double checking and ordering primers for transgenic endophyte study


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