User:Anthony Salvagno/Notebook/Research/2010/01/08/Ligation of Cap14 to Tether Construct

Here we go ladies and gents, this is the first try with working Anchor and Adapter of the ligation of the tether construct. Let's hope this works!

Ligation
Take out after 3 hours.
 * 30' - added 1uL
 * 60' - added 1uL
 * 90' - added 1uL
 * 120' - added 1uL
 * 150' - added 2ul

Gel Results
I have just learned that leaving SYBR Safe out can cause it to photobleach... duh! Also it is good for 6 months. Since I'm over 6 months and it's been sitting out for so long (I can't remember when I got rid of the box) this explains why my gels suck. I ordered some new stuff, but let's see how this gel comes out. Look really hard and you'll see: I gel extracted the band nearest the plasmid DNA band. Monday we'll find out if this worked!
 * 1) 1kb ladder
 * 2) 3x 1kb ladder
 * 3) ligation