Klapperich Lab:Notebook/Lab Meeting Notes/2009/09/29

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29 September 2009 Lab Meeting
‡ Announcements ‡ Flu R01:Integration † Sample Concentration (Lead: Jane, Team: Jaephil) † SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) † PCR - CMI (Lead: Qingqing) † HDA (Lead: Jaephil, Team:Sonali) '''‡C. diff Project''' (Cathie, Sonali, Satish Singh, His post doc ) ‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing) ‡ Agilent Automated Sample Preparation (Lead: Alex) ‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)  ‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks) ‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA) ‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)
 * Attending:
 * Missing:
 * Presentation:
 * No group meeting 10/6.
 * Invited speaker at group meeting on 10/13 - PLEASE Do NOT MISS - Dr. Qian Mei, post doc applicant.
 * PAPERS Drafted before MicroTAS
 * Posters for MicroTAS Due to CMK by 10/15
 * New design - address evaporation loss, Reagent delivery and storage solution. Consider marginating flows?
 * Up to 10^8 for positive control.
 * Jane working on the cell lysate control.
 * Main loss is at the outlet/sample collection.
 * Alexa Fluor-488 succinimidyl ester (Molecular Probes) stains the flu virus fluorescent.
 * The current problem is not how to visualize virus right now, because the design is clearly not optimized for collecting all the samples at the outlet. The major loss is from sample handling instead of from non specific binding to the material.
 * Jane is thinking outside the box. New chip design. Bacteria design is not working.
 * RNA extraction troubleshooting. Do deinhibition of monolith components. Test those with the Ambion RNAse kit. Jessie this week.
 * Team met last week to plan experiments.
 * New Designs of bigger channels are made, 35 to 70 μl total volume (MinCheol was Consulted). Downside here is the time to extract. Hussam to make spreadsheet of channel type vs. times for lysis, extraction, and elution.
 * Silica free SPE, and SPE free channel experiment were run, to be PCR'ed.Lambda phage assay.
 * Jessie worked with plasmid. Good std. curve. Not good predictor of the real sample amount. J will redo math with Sonali and Hussam to check.
 * QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR.
 * QQ: Paper back to QQ 9/29.
 * CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations.
 * made 40 cycle chip.
 * Success with 30 cycles, Qiagen Kit. Look at annealing temperature as per Alexis.
 * Integration chip for Qiagen RT-PCR one tube reaction. Control sample first, patient sample second.
 * Start planning R01 for Submission on 2/5/10. MM, JD, CMK.
 * Figures discussion.
 * Cathie needs paper for submission this week.
 * Meeting 12:30-1:30pm every other Wed. in my office.
 * This week meeting at 10:15 AM my office Wednesday.
 * Tracking sample bank.
 * Meeting 9am Monday 9/14 (every other week)
 * With Straws in Parallel at this point. Speeding up the assay.
 * Waiting for the machine.
 * Alex drafting abstracts.
 * Integration discussion on the meeting (9.23)
 * Virus concentration (dialyzed against PBS, no SERS signal, dialyze against water now).
 * paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli.
 * New experiments happening now.
 * IRB approved.
 * Cathie submitted the companion BU IRB form for exemption. Revised, still waiting.
 * 12 mos, 24 mos. working prototypes of SNAP.
 * Training on straw making concluded.


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