GroseLab:Protocols

GroseLab



Recipes

 * Common buffers
 * Common media
 * Stock solutions

DNA

 * TAQ PCR
 * Phusion PCR
 * Cloning

Protein

 * Western Blot

DNA

 * DH5α Competent Cells
 * Electrocompetent cells
 * Linear transformation

Protein

 * HIS tag purification
 * GST purification
 * Myc purification
 * FLAG purification

DNA

 * Competent Cells and Transformation
 * Quick Genomic DNA prep
 * Yeast Plasmid Prep

Protein

 * IP kinase assays
 * in vivo PASK phosphoryation assay
 * in vivo Ugp1 phosphorylation assay
 * his tag purification
 * GST purification
 * Myc purification
 * FLAG purification