IGEM:MIT/2007/Protocols

General Lab Protocols

 * General Lab Do's and Don'ts
 * Calculating Optical Density; Nanodrop Protocol
 * Making a Cast
 * Making Agarose Solution
 * Making an Agarose Gel
 * Preparing a Gel Electrophoresis
 * Running a Gel Electrophoresis
 * Making LB Amp Agar Plates
 * Innoculating LB with bacteria
 * Gel Imaging

DNA Work

 * Mini Prep
 * Mini Prep v.2.0
 * PCR Amplification
 * PCR Purification in a Microcentrifuge
 * PCR Cleanup
 * Double Digestion
 * Ligation
 * Purifying the DNA
 * Transformation
 * Colony PCR (OWW, Knight)
 * Phosphatase treatment of linearized vector