Berglund:PCR: Commonly Used Primers

SELEX primers

 * N30.2F: 5' gat aat acg act cac tat agg gaa tgg atc cac atc tac ga 3'
 * N30.2R: 5' aag ctt cgt caa gtc tgc agt gaa 3'

0 bases from the MCS

 * pcDNA3 Forward: 5'ATTAATACGACTCACTATAGGGAGACCC
 * pcDNA3 Reverse: 5'AGCATTTAGGTGACACTATAGAATAGGG


 * pGEXF1: 5'CTG GAA GTT CTG TTC GAG
 * pGEXR1: 5'CGT CAG TCA GTC ACG ATG

35 bases from the MCS

 * pGEXF2: 5'CGACCATCCTCCAAAATCGG
 * pGEXR2: 5'CCGTCATCACCGAAACGC

50 bases on either side from the MCS

 * pGEXF3: 5'ggg ctg gca agc cac gtt tgg tg
 * pGEXR3: 5'ccg gga gct gca tgt gtc aga gg


 * pUC19 F: 5'GGATGTGCTGCAAGGCGATTAAGTT
 * pUC19 R: 5'TTCCGGCTCGTATGTTGTGTGGAAT

100 bases on either side from the MCS

 * SP6F1: 5'gat atc aca gtg gat tta
 * SP6R1: 5'att tag gtg aca cta tag

Other

 * T7primer: 5'TAA TAC GAC TCA CTA TAG GG
 * pcDNA3 F1: 5'ATT AAT ACG ACT CAC TAT AGG GAG ACC C
 * pcDNA3 R1: 5’ AGC ATT TAG GTG ACA CTA TAG AAT AGG G