User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/26

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05/26/11

 * &#x2713; Cultures/minipreps/digests: KAH183/176fwd(6), KAH184/176fwd(6), KAH183/177fwd(6), KAH184/177fwd(6)

Minipreps > Check with E/ApaI digests > Note: ApaI site in the vector and in the PEST region of the RFP-Repressor gene

--> Observed bands do not match predictions. Recalculate assuming methylated ApaI site in the vector --> Makes sense, keep positive preps for transfections
 * KAH176 = 6421, 1423
 * KAH177 = 6614, 1423
 * 183/176 = 6421, 3806
 * 184/176 = 6421, 3614
 * 183/177 = 6614, 3806
 * 184/177 = 6614, 3614


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