IGEM:IMPERIAL/2007/Notebook/2007-9-2

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7

Construction of pT7-GFP

 * 1) 5 colonies grew in the ligation plate
 * 2) All 5 colonies were picked and each was grown in 5 ml of LB + Kan
 * 3) The 5 cultures were incubated at 37&deg;C overnight

Cloning of Biobricks (for Maxiprep)

 * 1) Placed 200 ml of LB in a 500 ml conical flask
 * 2) Innoculated 2 ml of culture into LB media in flask
 * 3) Incubated overnight at 37 °C


 * 2. BBa_I13422 [ptet-GFP]
 * 18. BBa_T9002 [plux-GFP]