User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/09

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] SDS-PAGE of cAMP precipitation
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Method

 * Put samples to 8% polyacrylamide gell

Results

 * Ponceau staining of cAMP precipitation on 8% Gel



Discussion

 * A band around 55 kDa is shown only concentrated in the cAMP pull down
 * This would be RII subunits showing that cAMP precipitation is succesful
 * cAMP treated beads pull down don't show any bands, indicating that cAMP prevented the binding of samples to cAMP bound to the beads


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