User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/03

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01/03/10

 * Assemblies: KAH117-119/MV2; KAH94, 95/ JDS42; KAH94, 95/ JDS43

Assemblies
 * 1) KAH117/MV2: (1) KAH117/(E/P)/7758 + MV2/(E/P)/4529 &#x2713;
 * 2) KAH118/MV2: (2) KAH118/(E/P)/7930 + "
 * 3) KAH119/MV2: (3) KAH119/(E/P)/8002 + "
 * 4) KAH94/JDS42: (4) KAH94/(E/P+Ad's)/5011 + JDS42/(BglII)/7468 &#x2713;
 * 5) KAH95/JDS42: (5) KAH95/(E/P+Ad's)/5083 + "
 * 6) KAH94/JDS43: KAH94/(E/P+Ad's)/5011 + JDS43/(BglII)/7478 &#x2713;
 * 7) KAH95/JDS43: KAH95/(E/P+Ad's)/5083 + "

> Digests (Fermentas FD)

--> For KAH94 & 95: also add old purified E/P digest to melted gel/DNA for column binding. --> Use 20 μL for final elutions.

> Measure conc.'s

> Ligations --> Use dsoligo adapters (Ad BglII/EcoRI and Ad PstI/BglII from 12/28/09) for JDS constructs

--> R.T./ 30 min.; Add 30 μL Z-DH5α


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