User:Mar/Notebook/2007-6-5

= protein extract fractionation (cont) =

Consulted with Bill Driscoll:


 * 1) the extract that will be prepared for ZOOMing (detergent + urea added) has to be of max 0.5-0.6 mg/mL concentration
 * 2) ZOOM recommends 1-2mg of total protein per run
 * 3) Bill suggests 2 runs of 300µL each of 0.6µg/mL extract
 * 4) originally we did 1 run of 400µL of extract in final volume of 4000µL (~650x5) after preparation for ZOOMing
 * 5) pool 4 sub-samples from new extracts to make up for 2x300 = 600µL
 * 6) we start on Monday of Jun-12