User:Karmella Haynes/Notebook/BioBrick cloning/2011/01/19

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01/19/11

 * &#x2713; Assemblies: KAH179, 180; KAH176, 177, 178/ MV8 (AY2 zeo vector)

Assemblies --> Clone Repressor expression constructs in the rev direction to test effectiveness of polyA BioBrick
 * 1) KAH179: (1) FC7/(E/S)/714 + (2) KAH175/(E/X)/~5421
 * 2) KAH180: HPK/(E/S)/516 &#x2713; + "
 * 3) KAH176/MV8 fwd: (3) KAH176/(E/S)/3191 + (6) MV8/(E/X)/4594
 * 4) KAH177/MV8 fwd: (4) KAH177/(E/S)/3384 + "
 * 5) KAH178/MV8 fwd: (5) KAH178/(E/S)/3395 + "
 * 6) KAH176/MV8 rev: KAH176/(E/S)/3191 + (7) MV8/(E/S)/4594
 * 7) KAH177/MV8 rev: KAH177/(E/S)/3384 + "
 * 8) KAH178/MV8 rev: KAH178/(E/S)/3395 + "

> Digests (Fermentas FD) --> Cut KAH176, 177, 178 w/ E/S/ScaI

> Measure conc.'s

> Ligations


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