840:153g:Projects/project4/2009/02/12

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Thursday 2/12
Josh and Casy
 * Came in early to start Agarose gel. Followed protocol discussed Tuesday.
 * Prepared the part pSB1A2 + BBa_R0080 as digested DNA and undigested DNA for running on the electrophoresis gel. Digested DNA with the enzymes Xba1 and Spe1.
 * Ran the gel for 30 minutes.
 * The gel showed that the digested samples formed two fragments, the smaller of which was about 200 base pairs. This confirmed that the desired part has been isolated.
 * Discussed preparing the plasmid for the incorporation of the aprE gene.

Oggie, Derek, and Katy
 * Oggie and Derek came in early to make Agarose gel.
 * We checked PCR product using gel electrophoresis.
 * Our + control worked but our DNA did not work.
 * On Tuesday we will be calibrating pipets and redoing our DNA extraction.

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