SD

Summary
Synthetic Dextrose (SD) Medium. Defined Minimal yeast medium

Ingredients
Carbon source:
 * Dextrose 20g/L

Nitrogen source:
 * Ammonium sulfate 5g/L

Vitamins:
 * Biotin 20μg/L
 * Calcium pantothenate 2mg/L
 * Folic acid 2μg/L
 * Inositol 10mg/L
 * Niacin 400μg/L
 * p-Aminobenzoic acid 200μg/L
 * Pyridoxine hydrochloride 400μg/L
 * Riboflavin 200μg/L
 * Thiamine hydrochloride 400μg/L

Compounds supplying trace elements:
 * Boric acid 500μg/L
 * Copper sulphate 40μg/L
 * Potassium iodide 100μg/L
 * Ferric chloride 200μg/L
 * Manganese sulphate 400μg/L
 * Sodium molybdate 200μg/L
 * Zinc sulphate 400μg/L

Salts:
 * Potassium phosphate monobasic 850mg/L
 * Potassium phosphate dibasic 150mg/L
 * Magnesium sulphate 500mg/L
 * Sodium chloride 100mg/L
 * Calcium chloride 100mg/L

Protocol

 * 1) Mix 6.7 g Difco YNB w/o Amino Acids with distilled water to 950 mL
 * 2) *If making plates add 20 g Agar in addition to YNB
 * 3) Autoclave (liquid cycle)
 * 4) * 250&deg; F, 22 psi, 30 minutes
 * 5) Add 50 mL 40% glucose (available from the kitchen)

Source
Adapted From:

F. Sherman, Getting started with yeast, Methods Enzymol. 350, 3-41 (2002)

or as a pdf: Getting started with yeast