IGEM:PKU Beijing/2009/Notebook/AND Gate 2/2009/07/21/Zhenglin Yang

1:00 Double digestion for purified T3 Polymerase PCR Production

8:00 Purify the digestion production

9:00 Ligation of T3 polymerase and TetR backbone

14:30 Transformation

16:15 Plate cultivation