JCA061606-Construction of key3 library 994

 PCR ca994F/ca996R on pSB1A2-J01129X   (~2.2kb, XbaI/DpnI, Neb2) Ligate, product is Lib994 library Transform TG1/pSB3C6-J01122 cells

ca994F	Forward XbaI EIPCR for key library CCTATtctagaGACCCAAAAGCANGAGGNNNNNNTAGTNGGTGGTTAATGAAAATTAACTTACTTACTAG ca996R	Reverse XbaI EIPCR for lock and key CGATGtctagaAGTGCTCAGTATCTCTATC

construction failed, potential new scheme:

PCR ca994F/G00101 on pSB1A2-J01129     (bp, XbaI/PstI, Neb2) Sub into pSB1A2-R0040                  (SpeI/PstI) Product is Lib994

Alt new scheme: PCR ca994F/ca670R on pSB1A2-J01129     (bp, XbaI/AlwN1, Neb2) Sub into pSB1A2-R0040                  (SpeI/AlwNI) Product is Lib994

construction of klenow extended 994Library digest PCR product (XbaI/PstI, Neb2, small frag cleanup) Paste into pJ23006-I13522 (XbaI/PstI, Neb2, Large-2334) Product is pJ23006-I13522-994Library

