User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/10/05

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Dissect 3 sets of testis
 * 8 from Tdt-Myb
 * 2 x 8 from GFP-Myb, H2Av-RFP
 * Fix
 * 30' in 4% Formaldehyde at RT
 * Wash 4 x 5' at RT
 * Block
 * 1 hr at RT in 2% NGS
 * Primary Antibody
 * Tdt-Myb with 1:1000 anti-mRFP O/N at 4°C
 * GFP-Myb with 1:5 anti-BAM O/N at 4°C
 * GFP-Myb with 1:100 anti-SA O/N at 4°C


 * }