IGEM:Stanford/2009/Notebook/Personal Notebook/2009/08/06

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] Suzie's iGEM Project
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Daily Work
Goals
 * Morning:
 * vBOL tutorial polishing
 * purchase omnigraffle
 * possible wiki template work
 * Afternoon
 * Red papers for NO/SoxR affinity and Th-17 to NO pathway

Project / Accomplishments
 * Long review of entire cloning process
 * Learned to check sequences w/ Ape and order form (fwd and rev)
 * Broke down project steps
 * Finished new draft of presentation and went over it w/Jerome
 * Now contact physician and clinician
 * Finished vBOL presentation
 * Grew liquid cultures of B0015 and B0034, and part 53 and glycerol stock 9
 * Started ligation for 1.1b (device one, first round of cloning, part b)
 * Right cycle on PCR?

Problems
 * A little unsure about ligation protocol.... which PCR cycle? We chose ligation...

Alternative Solutions

Events

 * 3pm meeting w/ Jerome about updated presentation... Feedback
 * More clear about age groups- is this when it starts?
 * Why industrial countries targeted? Sterile environments....
 * Where does it take place?
 * Genetic component of the disease?
 * Mouse model of IBD to test linear relationship of Th-17 and Treg cells
 * Colon slide- show parts w/ larger picture and then transition into video by itself
 * Naive cd4+ cells do not show up in the video on the screen
 * Positive feedback between t-cells and differentiation of naive cd4+ cells
 * Trp analog levels
 * Device one diagram: sensor will end at SoxS, then generator is entire sequence
 * Constitutive export system..
 * Constitutively expressing everything besides il-6
 * Missing consistent background of T-cells... larger description of immunology and mediated immune cells
 * Simple, fun animation... like the french cartoons
 * Take out th1 and th2 cells in picture
 * th-17 to t-reg cells stemming from Immune System
 * Go straight to the problem
 * Explain T-cells- what do they do? Like a detector....
 * Signal another cell to kill the pathogen...
 * talk about the recruitment of b-cells
 * Cartoon is not a system level diagram
 * New system level diagram- simple but w/ external features like b-cells and dendritic cells
 * Also, upstream of inflammation and immunosuppression symbols in diagram... disease...
 * Reuse presentation diagrams
 * Missing level between box pictures and basic parts diagram
 * Maybe boxes such as SoxR/S system= NO sensor
 * Introduce vBOL later, but not too much time
 * suicide device?
 * schematics w. numbers/letters
 * Other questions... do people take RA for treatment? Il-6? Methyl tryptophans for IDO
 * Check out other uses for the products


 * From here
 * Contact clinician from here
 * Presentation works from here

Weekly Goals

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