User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/09

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Woohoo!!! Transformed strain is a win!!!
    -H2O ---> 10μl
 * When I arrived in the morning, I saw that the strains that I plated yesterday grew well, so I inoculated them in 4mL of liquid LB medium with 4μL of tetracycline. Incubation for 7 hours at 37°C.
 * Afterwards, I extracted plasmid by using the Roche High Pure Plasmid Isolation Kit. The tubes are labeled as: plasmido purif. lucif + TT Mariana 9/06/10 (1-G).
 * Finally, I did a restriction for a total of 20μL as follows and labeled it as Restriccion ECO RI y PST Mariana (1-H):

-Buffer 2 --> 2μl

-BSA --> 1μl

-DNA ---> 5μl

-ECOR1 -> 1μl

-PST1 --> 1μl


 * Restrictions were incubated at 37°C ON(overnight).


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