TempliPhi

Overview
A kit for in vitro amplification of plasmid DNA for sequencing. It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps. It is also useful for sequencing low copy plasmids which give poor yields from minipreps. This kit is based on the &phi;29 DNA polymerase and uses rolling circle amplification.

Materials

 * illustra TempliPhi™ 100/500 Amplification Kit

Specific protocols

 * Endy:Templiphi
 * Knight:TempliPhi