Talk:20.109(F09): Mod 2 Day 6 Readouts of DNA, Protein

T/R lab
INFORMATION TO BE FILLED IN BY EACH GROUP All mutant candidates were digested with a cocktail of Nde and Mlu in 1X NEB3 for 45 minutes at 37° then loaded on a 1% agarose gel (1XTAE)

'''Here is your Western data. If you'd like to see the blots themselves, they'll be in the lab at the front bench. The "?" after your team color are associated with teams who labeled their containers on the lids rather than the tubs. These blots were possibly mis-designated during the washing and scanning of these blots since the lids were separated from the tubs. If you'd like to note which of the 2 preparations of primary antibody you used, you can do so in the label under the blot's image'''

Digest Sample info
Please fill in the info below for your team for others to refer to:



Digest Images
Candidates were digested for 55' at 37 &deg;C and run on a 1% agarose gel. Each gel was photographed at two different exposure times - use whichever one makes your DNA easier to see. Also see the second T/R gel for how control DNA (with and without stuffer fragment) runs.





Western images
Below are your Westerns. (Please crop out your image for use in the research article.) Some information may be lost in the digital images, so feel free to look at the original blots after journal club to get a good sense of where your bands are.