User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/08

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Western Blot
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary
Western blot Check cells for stimulation

Method
Samples were loaded 40 μL, with 15 μL prestained protein marker in the first lane, according to the schedule below. Gell was run for 45-60 min @ 200 V until loading buffer had left the gel (+5 min). Proteins were transferred to membrane 1 h @ 100 V on ice.

Results
Pierce Determination VASP 080310

Conclusion

 * ✅ Ow..


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