User:Karmella Haynes/Notebook/BioBrick cloning/2010/07/26

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07/26/10

 * &#x2713; Sequencing order: fly mCherry mutagenesis clones

Sequencing order (Genewiz standard) --> Forward primer = M13F(-21) (Genewiz) --> Reverse primer = M13R (Genewiz)
 * 1) flymCh-01 F: good
 * 2) flymCh-01 R: good
 * 3) flymCh-02 F: not checked
 * 4) flymCh-02 R: not checked
 * 5) flymCh-03 F: not checked
 * 6) flymCh-03 R: not checked
 * 7) flymCh-04 F: not checked
 * 8) flymCh-04 R: not checked

--> Keep clone #1 --> Next, add BioBrick ends via PCR and clone into V0120


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