User:Tara K. Luckau/Notebook/Team ConGen/2011/06/24

{| width="800"
 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
 * style="background-color: #9DB68C" align="center"|  |Main project page
 * style="background-color: #9DB68C" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

PCR

 * multiplex looks good in some PCRs (when run next to uniplexes), but not in others (when scaled up for frag submission)
 * try full gradient (temperature and buffer) of multiplexed reaction


 * [[Image:20110624 PCR.jpg|800 px]]

Gel

 * [[Image:20110624 Gel.jpg|800 px]]
 * not favorable results
 * lane for Buffer E, 64.3° consistent with 23 June 2011 PCR/gel
 * in most lanes, can see the Scun9/10/11 band
 * some lanes tend to have larger (Scun15, 22) or small amplicons (Scun2, 3)


 * given that this doesn't seem to be able to get 'better', will submit yesterday's PCR to UAGC
 * should be able to see which loci aren't amplifying, which loci are dominant


 * might be able to split the multiplex into two separate multiplexes
 * looking at Gradient Summary, 13 June 2011:
 * Scun3, 9, 11 - Buffer D, 64°C
 * Scun2, 10, 15, 22 - Buffer D, 56°C
 * Scun10, 15, 22 - Buffer G, 61°C


 * }