User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/18

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Friday 18th June 2010
Today I performed 2 double restrictions:


 * GFP cut with XbaI and PstI


 * Plasmid 18 cut with EcoRI and PstI

as follows:

For GFP

Reactive...........1x [ul]

DNA................25

BSA..................1

Buffer2............4

H2O................7

PstI................1.5

XbaI...............1.5

Total...............40


 * For plasmid 18

Reactive...............1x [ul]

DNA....................10

BSA......................1

Buffer2................3

H2O...................13

EcoRI-HF.............1.5

PstI......................1.5

Total..................30

I left the digestions in the incubator overnight.


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