20.20(S10):Teacher Resources

2020(S10) Lecture:week 1

 * 1) Project Runway
 * 2) *paper, paperclips, tape, pennies, pencils, scissors
 * 3) iGEM reviews
 * 4) *copies of ppts from iGEM talks
 * 5) take apart
 * 6) *hard copies of Comic to cue homework assignment
 * 7) *tape recorders (16), tools (#0 phillips, scissors, wire cutters), bags to store parts, duct tape was suggested to collect small pieces, sharpies/paper/pens
 * 8) *link to pre-survey

2020(S10) Lecture:week 2

 * 1) tape recorder reassembly: bring electrical or duct tape next time to re-attach broken wires, bring 2xAA batteries, tape to play when players successfully reassembled.
 * 2) poster session studio: need 18"x24" paper, printer paper, felt tip markers, pencils, rulers, masking tape, sharpies
 * 3) play decide: print out games (genetic testing) onto proper colored sheets but do not cut. Instead, distribute sheets with scissors to allow students to cut and save just the cards they found most relevant. Depending on room, may also want to print out rules of play since screen not easy to see and tables too crowded for computers. Leave enough time so discussion can be recapped as well as policy.

2020(S10) Lecture:week 3

 * check list in binder

2020(S10) Lecture:week 4

 * 1) DNA isolation
 * 2) *Disposables: small white cups, larger clear plastic cups, coffee stirrerwater, Coffee filters, ziplock bags (sm for expt, lg for disposal), papertowels for spills
 * 3) *Reagents: strawberries, liquid soap, NaCl, isopropanol
 * 4) casting gel
 * 5) *tupperware: small rectangular (9.5 fl oz), larger rectangular for running (3 cup size)
 * 6) *Lego: for each need 1 (10 by 1) or(10 by 2) piece, 2 (2 by 3) piece, 6 (1 by 2) tiles
 * 7) *for each gel: 1/2 L bottle of water, salt, baking soda, aquarium pH paper, microwave, agar agar
 * 8) *misc: masking tape, scissors, coffee stirrer, paper coffee cup for melting in microwave
 * 9) loading gel
 * 10) *glycerin, food coloring, coffee stirrer
 * 11) running gel
 * 12) *for each gel: 5 9V batteries
 * 13) FooCamper's Guide
 * 14) *autoclaved beads - VWR 26396-521 for plating the cells.
 * 15) *hole punch to punch out the dNA
 * 16) *One-shot competent cells (I've gotten Invitrogen TOP10 previously, but NEB or whatever also works)
 * 17) *Ice in a bucket - for thawing cells
 * 18) *Glass of water, microwave and thermometer - for heat shock
 * 19) *SOC/LB media without antibiotic - for use after the heat shock - I aliquoted this out for them beforehand
 * 20) *Tape - so they can tape their tubes to a warm place to grow for 1 hr, or they can just hold them in their hands
 * 21) *Amp Plates - for people doing RFP and luciferase transformations
 * 22) *Liquid Amp media - For media, I mixed LB-Amp media pack - Invitrogen Q600-20 $169.00/20 pouches with bottled water and added isoamyl alcohol to 5mM - use this with the banana cultures
 * 23) *14 mL culture tubes - VWR 60819-728

Poetry
Not long ago when biology meant looking and prodding and fixing what’s bent there were armies of scientists working away to list what they found and to know life’s display But not all was well as they tried to discover how life could be programmed since some but not other experiments worked and each person designed their template for learning as they were inclined “Share!” said the engineers “and try to remember that others will use your work only whenever the tools you develop are standard and simple think of most screwthreads and think of the wheel. Maybe in this way life by design could work out of the box and others will find lots of interesting ways to build up from the bottom making useful new parts then new systems. No problem!” By combining devices in new and fun ways the biologists builders could spend their workdays learning what’s out there and making new widgets to responsibly meet the needs of our planet.