Richard Lab:Microorganisms & Media

Back to Richard Lab Main Page =Prokaryotic=

E. coli NEB 5a

 * A DH5-alpha derivitive supplied by New England Biolabs
 * Used for general cloning including Bgal selection.
 * Electrocompetent cells are kept in the -80°C Freezer in Dr. Demirci's lab.
 * Ask Mike

E. coli DB3.1

 * These clones are subject to ccdb lethality
 * Plasmids with the ccdb gene surrounding the MCS are used to select for inserts
 * DB3.1 cells with non insert plasmids will die
 * Ask Mike

Lactobacillus plantarum PA-28

 * Commercially available silage inoculant
 * Found in "Biomax 5" by CHR Hansen biosystems
 * Ask Mike

Lactobacillus plantarum K-270

 * Commercially available silage inoculant
 * Found in "Biomax 5" by CHR Hansen biosystems
 * Ask Mike

Lactobacillus plantarum LP-401

 * Commercially available silage inoculant
 * Found in "Biomax MP" by CHR Hansen biosystems
 * Ask Mike

Lactobacillus plantarum LP-1

 * Commercially available silage inoculant
 * Found in "Biomax MP" by CHR Hansen biosystems
 * Ask Mike

Lactobacillus plantarum MD007

 * is a knockout of the alanine racemase gene
 * cannot grow on MRS media without supplemented D-alanine
 * used for promoter probing with the pNZ7120 promoter probe vector
 * Bron, P.A., S. M. Hoffer, I. I. Van Swam, W. M. De Vos and M. Kleerebezem. 2003.  Selection and Characterization of Conditionally Active Promoters in Lactobacillus plantarum, Using Alanine Racemase as a Promoter Probe.  Applied and Environmental Microbiology 70 (1): 310–317
 * Ask Mike

Lactococcus lactis MG1363

 * Contains pNZ7120 promoter probe vector
 * Can be selected for based on ampicillin resistance
 * grow on M17 media supplemented with 0.5% glucose
 * Bron, P.A., S. M. Hoffer, I. I. Van Swam, W. M. De Vos and M. Kleerebezem. 2003.  Selection and Characterization of Conditionally Active Promoters in Lactobacillus plantarum, Using Alanine Racemase as a Promoter Probe.  Applied and Environmental Microbiology 70 (1): 310–317
 * Ask Mike

Pedicoccus pentosaceus PC-3

 * Commercially available silage inoculant
 * Found in "Biomax MP" by CHR Hansen biosystems
 * Ask Mike

=Eukaryotic=

Saccharomyces cerevisiae NRRL Y-2034 (ATCC 24702)
Back to Richard Lab
 * Obtained from USDA-ARS Culture Collection
 * Culture maintenance protocol
 * Ask Megan

=Media=