IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/26/Zhangs

Miniprep 12 samples: CI Terminators: 1-23L, 1-4H RBS: 1-2I, 1-2G, 1.2M, 1-5J, 1-5N, 1-1J, 1-1H, 1-2K, 1-11N The 12 plasmid samples stored in -20°C

Enzymatic digestion Samples: CI, 1-23L, 1-4H System: For CI insert:

For terminator vectors 1-23L & 1-4H:

Add CIAP into 1-4H & 1-23L vector digestion product. Electrophoresis the digested product. Samples loading order: CI1, CI2, M, CI plasmid (Control) Result reveals that the double-digestion failed. The double-digested fragments can’t be identified out in electrophoresis causing the concentration of plasmids in this enzymatic digestion system is too low.

Put 3 samples (CI, I-4H, 1-23L) into the shaker