User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/16

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] SDS-PAGE lysates, Cell splitting
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Summary

 * 2x SDS-PAGE
 * NOTE: After 60 min. the SDS-PAGE was put to ice and the voltage was turned from 200 V to 100 V, to latter because of limitation in the amount of people running wet blots.
 * Both gels were blotted using the NuPage wetblot system, 2 h @ 30 V
 * Cells were splitted to D9 P28
 * Cells in 24-wells were put to S0

Results

 * Ponceau staining of blots

Discussion

 * ✅ Ow..


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