SBB09 Construct40

Construction of upaG (long) basic part PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI) Sub into pBca9495KC-Bca1144#5               (EcoRI/BamHI, 2974+910, L) Product is pBca9495KC-M10054 {<upaG_long!}

js001F Forward biobricking of upaG (long) CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg js002R Reverse biobricking of upaG (long) CTGATggatccCCACtgaataccggcaccga

JCA: You designed a { }, you need a stop codon in reverse oligo. See revision:

Construction of upaG (long) basic part PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI) Sub into pBca9495KC-Bca1144#5               (EcoRI/BamHI, 2974+910, L) Product is pBca9495KC-M10054 {<upaG_long!}

js001F Forward biobricking of upaG (long) CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg js002R Reverse biobricking of upaG (long) CTGATggatccTTACCACtgaataccggcac