User:Tk/Notebook/MF/2009/02/27

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
 * style="background-color: #F2F2F2" align="center"|  |Main Page
 * style="background-color: #F2F2F2" align="center"|  |Main Page


 * colspan="2"|
 * colspan="2"|

MF Recombination Cassettes

 * Required parts
 * constitutive promoter: possibly use the B. subtilis constitutive (K143012)
 * CIRCE element promoter
 * CAT cassette (J70026)
 * PheS mutant gene
 * modified CFP
 * MF deletion region homology parts
 * ME end parts (M1000; M1001)
 * optional/next parts
 * recE
 * recT
 * YFP
 * RFP
 * plasmid backbone (J70027)


 * Transposon constructs, made with insertions into existing TT01 part
 * const promoter + CFP
 * CIRCE + CFP
 * cat cassette; PheS cassette in same construct


 * recombination parts
 * transposon insertion of CIRCE + recT + recE
 * homology - cat cassette - pheS cassette - homology
 * do we need thio primers for PCR of this?
 * homology - homology for testing deletion


 * plasmid tests
 * plasmid + cat cassette (J70027 + J70026)
 * plamid + constitutive + CFP


 * }