Jasieniuk:Notebook/Apomixis in hybrid Rubus/2010/10/06

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Apomixis in hybrid Rubus
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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ABI run and allele calling

 * Plates RUB101001A3 and RUB101001B ran fine, except that the machine ran out of polymer for the last run.
 * Refilled the reserve syringe with polymer.
 * Made new plate file, RUB101001C, that was columns 11&12 of plate RUB101001B, but rotated around into columns 1&2.
 * Ran RUB101001C. Run # 6271
 * Created GeneMapper project RUB101001, containing runs 6256, 6260-6269, and 6271
 * Updated consolidated genotypes file: [[Media: RUBapomixis101006_Genotypes_Table.xlsx]]

Finding samples on old seed plates that worked

 * File listing old seed plate samples that worked before: [[Media: working_from_old_seed_plates.xlsx]]
 * No PCR done for old seed plate 2? Might try entire plate
 * These will be included in next round of do-overs


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