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Jenn Brophy

Circularly Permuted ompG
Source: E. coli MG1655 genomic DNA Before starting design, you should read this tutorial: A Guide to Circular Permution In general, you should clone the bits and pieces out of genomic DNA first, subclone and sequence those bits, then assemble them into full length in a separate step. You can use either SOEing as described in the tutorial to do the assembly, or use standard assembly.

The reference for structural information about ompG is: PMID: 18473482