Template:SBB10 ConstructionFiles KaiM sbb27

sbb27: CA42 rep Protein

PCR KRM001 and KRM002 on pEC22-CA42 (987 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144           (EcoRI/BamHI, 2472+910, L) Product is pBca9523-sbb27         {rbs.repCA42!} KRM001  Forward oligo for cloning of rbs.repCA42! ccataGAATTCatgAGATCTcccggagagaatcgtaacac KRM002  Reverse oligo for cloning of rbs.repCA42! ctgatGGATCCctattttccgcttttccag



JCA Notes

 * correct