IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/20

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

July 20th, 2010

 * Prepared gel electrophoresis dyes
 * Digested CFP construct for gel electrophoresis
 * Ran three gels with CFP construct
 * Excised band thought to be CFP construct
 * Repeated CuSO4 treatments on E. coli
 * Aliquoted 1 ml of the CuSO4 treated cultures (above) to microcentrifuge tubes, centrifuged, and observed pellets for fluorescence with UV lamp
 * Read optical density of E. coli growing in M9 media
 * Prepared an overnight culture of GFP in LB Carb broth
 * Autoclaved biohazard waste and pipette tips


 * }