User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/21

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12/21/09

 * &#x2713; Assemblies: KAH120-125 in MV2; KAH135
 * &#x2713; Oligo annealing: Joe's "Strept apt" tag ( Forgot kinase treatment, do tomorrow )

Assemblies
 * 1) KAH120/MV2: (1) KAH120/(E/P)/7930 + MV2/(E/P)/4529 &#x2713;
 * 2) KAH121/MV2: (2) KAH121/(E/P)/8102 + "
 * 3) KAH122/MV2: (3) KAH121/(E/P)/8174 + "
 * 4) KAH123/MV2: (4) KAH121/(E/P)/8002 + "
 * 5) KAH124/MV2: (5) KAH121/(E/P)/8174 + "
 * 6) KAH125/MV2: (6) KAH121/(E/P)/8246 + "
 * 7) KAH135: (7) Gal4DB-PEST(KAH55)/(S/P)/4146 + EED/(X/P)/1401 &#x2713;

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

Oligo annealing
 * 1) Strept apt (Joe's oligos, NotI sticky ends)


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