IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/05

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9/5/10
10:15 am

miniprep promega kit

gas vesicles: 317.2 ng/μL

B0015: 185.5 ng/μL

B0034: 185.9 ng/μL

11:10 AM

Digestion

ArsRSDM2B0015

B0034

pSB1C3

incubate for 15 min at 37°C, inactivate for 20 min at 80°C.

2:45 PM

ligation

incubate at room temp for 30 min, inactivate at 80°C for 20 min

3:45 PM

Transformation

50 μL cells, 2 μL ligation product. 10 min on ice, 45 sec at 42°C, 2 min on ice. Add 1 mL LB, recover for 1 hour shaking at 37°C.

5:00 PM

plated 250 μL cells on Cap plate. incubate at 37°C


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