IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 9

Transformation of OMPA loop his and strep (8/14/07)

 * 1) Add 1 µl of the DNA solution directly to the cells. Stir gently to mix.
 * 2) Place the tubes on ice for a little more than 5 minutes
 * 3) Heat the tubes for about 45 seconds in a 42°C water bath; do not shake
 * 4) Place on ice for 2 minutes
 * 5) Add 250 µl of room temperature SOC Medium to each tube
 * 6) Shake at 37°C (300 rpm) for more than 30 minutes.
 * 7) Use sterile spreaders to spread the bacteria on an LB Carb plate.

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Liquid Cultures for OMPA loop strep (8/17/07)

 * 1) Prepared liquid cultures
 * 2) 5 ml LB
 * 3) 5 ul amp
 * 4) single colony

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Streak Plate for OMPA loop his (8/17/07)

 * 1) Used multiple streaks to dilute the plate, which was a lawn of bacteria with nearly indistinguishable colonies.