IGEM:Peking University/2008/Notebook/Group 2/2008/06/20

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 * style="background-color: #EEE"|[[Image:PKU_logo08_01.jpg|150px]] Group 2
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Primer design

 * Primer 1
 * Primer name: F-HindIII-Apal-Gal4
 * Sequence(5'to3'):CCCAAGCTTGGGCCCAAGATGAAGCTACTGTCTTCTATCGAACAA
 * Tm:64.9°C
 * Primer 2
 * Primer name: R-EcoRI-Spel-Gal4
 * Sequence(5'to3'):GGGGAATTCACTAGTATTTTACTCTTTTTTTGGGTTTGGTGGGGT
 * Tm:61.3°C

PCR

 * conditions


 * Enzyme cutting-pPT1-XbaI-20 μL


 * }