BCA assay

goal: quantify amount of espA protein so that we know how much is stuck to the maxisorp plate, and so that future experiments would not be arbitrary values.

do everything in triplicate

use PBS as buffer

1. make 50:1 A:B of BCA solution 2. put in total of 200ul of solution plus either BSA or espA 3. for BSA --> increments of [0 .25 .50 .75 1.00] mg/ml 4. for espA --> start with 10ul and then dilute it 10X and then 100X 5. allow to incubate for 30minutes 6. OD562 reading on the Tecan 7. generate a standard curve of OD versus mg/ml for the BSA 8. approximate the concentrations for the espA protein.