User:Karmella Haynes/Notebook/BioBrick cloning/2011/03/24

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03/24/11

 * &#x2713; Cultures: SP1AB (KAH020) and p65 (KAH017) site-directed mutagenesis clones

Minipreps > Check via sequencing

3/25/11 Sequencing --> Order: Genewiz standard --> Note: sequence discrepancies same as before; probably due to mutations carried by U2OS


 * 1) KAH020-1 P0001: good; PstI #1 successfully mutated; discrepancy at bp 230 (A -> G); keep this clone
 * 2) KAH020-1 P0002: good; discrepancy at bp 1141 (A -> G)
 * 3) KAH020-2 P0001: same as KAH020-1
 * 4) KAH020-2 P0002: same as KAH020-1
 * 5) KAH020-3 P0001: same as KAH020-1
 * 6) KAH020-3 P0002: same as KAH020-1
 * 7) KAH020-4 P0001: same as KAH020-1
 * 8) KAH020-4 P0002: same as KAH020-1
 * 9) KAH017-1 P0001: complete mutagenesis failed; still has second PstI site; keep this one and re-do s.d. mutagenesis w/ same primer (checked primer seq, it's correct)
 * 10) KAH017-1 P0002: "
 * 11) KAH017-2 P0001: complete mutagenesis failed; still has second PstI site
 * 12) KAH017-2 P0002: "


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