Jhdavis::PeptideCleavage


 * 1) Transfer from block to glass scintillation vials
 * 2) Take block out of peptide sysnthesis hoot (hard to lift and easy to break)
 * 3) In hood, remove cam from well
 * 4) Using p1000 with cut tim, use 1 mL EtOH to transfer resin to glass scint. vial.
 * 5) Pipet off extra ethanol -> EtOH waste
 * 6) Let dry o/n.


 * 1) Cleavage
 * 2) There is a choice of scavengers (EDT seems good for everything) - see choice list in the hood.
 * 3) For peptides containing met, 94% TFA, 1 % TIS - thioisopropylene, 2.5% water, 2.5% EDT) - need 5 mL per peptide
 * 4) ~2 hrs. with shaking (put ether on ice during this time)
 * 5) Filter off resin - using glass wool in the pipette (pre-chill pipette/peptide)


 * 1) Wash
 * 2) Wash 4x in total with ether
 * 3) Spin 3000k for 10 min (swinging bucket with top sealed)