DpnI

Purpose

 * To digest (Adeno) methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.

Procurement

 * Can be ordered from NEB.
 * Typically stored at -20&deg;C.

Use

 * Use in NEBuffer 4. However, can be added directly to PCR sample.
 * Incubate at 37&deg;C.
 * Heat inactivate by incubating at 80&deg;C for 20 minutes.
 * DpnI cleaves hemi-adenomethylated dam sites 60X more slowly than fully methylated. NEB FAQ
 * Plasmids derived from Dam+ e.coli strains will be a substrate

Relevant protocols

 * Site-directed mutagenesis

Safety

 * None known.

Contact

 * Reshma Shetty