IGEM:Stanford/2009/Project Homeostasis/sequences

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Device 1
RBS – SoxR gene – Terminator – Spacer – SoxS Promoter click here

SoxR gene click here

SoxS Promoter click here

RBS - Blh gene click here

RBS - RALDH II gene - Terminator click here

hIL-6 hlyAs click here

Primer Design Guidelines

 * Primer Design
 * Guidelines to Create Primers
 * [[Media:Mutagenic_Primer_Kit.pdf | Designing Mutagenic Primers (see page 10)]]

Resources
Home Page
 * National Center for Biotechnology Information (NCBI)- Get Your Sequences

Prefix and Suffix Help
 * Parts Registry of BioBricks- Help with Prefix and Suffix

Analyze Your Sequence
 * IDT Integrated DNA Technologies- Check for %GC (40-60) and Melting Temp. (52-68C)

Analzye both Primers
 * NUPACK Nucleic Acid Package- check both primers for hairpins and trouble spots (No Red!!)

Download here
 * ApE- A Plasmid Editor- Useful tool for checking sequences and finding reverse complements

Design Primers Here
 * Ginkgo BioWorks- creates primers w/ BioBrick additions

Archived Documents and Work

 * Past Primers and Sequences
 * Finalized Original Primers
 * Original Primers