PIPES

piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757

A Good buffer Good66 Good74  Blanchard84.


 * pKa at 25C of 6.8 (a second one at pH 3 is not of interest)
 * usable buffering range of 6.1 to 7.5
 * molecular weight 302.4
 * &Delta;pKa/&Delta;T = -.0085

Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized.


 * 1) Good66 pmid=5942950
 * 2) Good74 pmid=4206745
 * 3) Blanchard84 pmid=6717292

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