Lactobacillus planarum miniprep

Overview
Method to miniprep plasmid DNA from Lactobacillus plantarum

Procedure

 * 1) Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase.
 * 2) incubate at 37°C for 15-30 minutes.
 * 3) Add Qiagen P2 buffer and continue miniprep as usual.
 * 4) Verify plasmid existance by sequencing or running digests on gel

Contact

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