User:Jessica Karen Wong/Progress

T9002
Output Measurement Kit (for inverters, terminators, etc) Using GFP

 T9002


 * F2620 has Mfe/BB prefix tails
 * Ligated into 3K3 and did correct transformation into DB3.1
 * Waiting on seq verification
 * E0240 has been PCR'ed with tails, looks the right size
 * Digested E0240 S/N to ligate into F2620
 * Will ligate into the correctly seq'ed F2620 colony

E0240
Promoter Tester with GFP

 E0240


 * Part is built
 * PCRed with Mfe1 and Spe1 tails
 * Successfully ligated into 3K3 - Sequence verifies that it's scarred 7/19
 * Inserted a BB site between plasmid and RBS
 * Made both E/S and E/X versions
 * Has a 1bp mutation around 700
 * Did mutagenesis and got a good number of colonies
 * EX version has been confirmed correct - 8/9
 * Made overnights to seq more ES colonies

I2057
Promoter tester with RFP

 I2057

Ligated J116, J100, and R0040 into EX version from PCR
 * Part is built (sequence verified)
 * PCR-ed with Mfe1 and Nsi1
 * Ligated in 3K3 (seq verified scarred) - 7/19 chose colony #4
 * Inserted a BB site b/t plasmid and RBS
 * Created both E/X and E/S versions
 * E/S sealed w/ Not1 is seq verified - 8/6
 * E/X sealed w/ Not1 is seq verified - 8/6 and 8/10
 * R0040 in both EX and ES versions is perfect - 8/9

I2055
RBS tester with GFP

 I2055


 * Built and scarred - 7/31 and 8/2
 * Inserted a BB site b/t Promoter and GFP
 * Made both E/X and S/X forms
 * Sealed both EX and SX with Not1 - waiting on seq
 * Inserted B0034 and B0032 into both EX and SX versions of PCR (and hopefully prep), also B0030, B0031 into SX PCR
 * I2055-SX-B0031 is seq verified 8/9

I2056
RBS tester with RFP

 I2056
 * Formerly called blue


 * Built and scarred - 8/6, 8/10
 * Inserted a BB site b/t promoter and RFP
 * Created both E/X and S/X
 * Sealed both SX (verified 8/14) and EX (8/14 and 8/15)
 * Putting in B0034 and B0032 into both EX and SX versions