User:Howard Boland/Notebook/Art from Synthetic Biology/2010/11/11

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Digestion pSense66-Ligated, Transformed and Purified

 * 1) .5µl 100xBSA
 * 2) 1µl 10xNEB#3
 * 3) 2µl PstI
 * 4) 30µl pSense66-Ligated(pBR322)-Transformed
 * 5) 17.5µl H20

Place in 37ºC Incubator for 2 hours.

Gel 1% Digested Plasmid and PCR

 * 1) Lane 1: 10µl 1kb NEB Quick Ladder
 * 2) Lane 2: 50µl pSense66 Plasmid Digest (PstI)
 * 3) Lane 3: Blank
 * 4) Lane 4: Blank
 * 5) Lane 5: Blank
 * 6) Lane 6: Blank
 * 7) Lane 7: Blank
 * 8) Lane 8: 50µl PCR Product (2300bp) Directly Digested with PstI (Not purified prior to digestion hence the additional bands).



Plating out pSense66 stock
I plated out 2 Kanamycin plates using the pSense66 stock obtained from overnight growth.


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