User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/14

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12/14/09

 * &#x2713; Minipreps: digests for 12/12/09 minipreps
 * &#x2713; Cultures: KAH123(3), 124(3), 125(3)
 * &#x2713; Assemblies: KAH126-133 into MV2

Minipreps > Check with E/P digests

--> 37°C/ ~15 min.

Assemblies
 * 1) KAH126/MV2: (1) KAH126/(E/P)/1880 + MV2/(E/P)/4529 &#x2713;
 * 2) KAH127/MV2: (2) KAH127/(E/P)/1880 + "
 * 3) KAH128/MV2: (3) KAH128/(E/P)/1880 + "
 * 4) KAH129/MV2: (4) KAH129/(E/P)/1880 + "
 * 5) KAH130/MV2: (5) KAH130/(E/P)/2864 + "
 * 6) KAH131/MV2: (6) KAH131/(E/P)/2867 + "
 * 7) KAH132/MV2: (7) KAH132/(E/P)/1688 + "
 * 8) KAH133/MV2: (8) KAH133/(E/P)/1532 + "

> Digests (Fermentas FD) --> Triple digest KAH130 & 131 w/ E/P/ScaI

> Measure conc.'s

> Ligations


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