User:Matthew Whiteside/Notebook/Fumigatus Microarray/2009/03/19

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Analysis of A.nidulans metabolic network
Using the all genes that were DE by T test (p-value 0.05), I reran the correlated subnetwork (jActiveModule) search using the A.nidulans metabolic network.

Interesting subnetworks
1. anthranilate (AN) metabolite.
 * DE enzymes connected to AN.
 * AN1857 (Afu4g09840) & AN5952 (Afu2g10360) - kynureniases, EC 3.7.1.3, catalyze kynurenine → AN + alanine.
 * These are up-reg at 4h, down-reg at 6h.
 * AN3634 (Afu4g11980) - anthranilate phosphoribosyl transferase (biosynth of tryptophan + others)
 * up-reg at 6h.
 * Pathway:
 * AN is used in tryptophan biosynthesis.
 * AN is also metabolite of tryptophan metabolism (kynurenine is intermediate).
 * AN is intermediate of chorismate biosynth. Chorismate is a precursor to siderophore, ubiquinone, folate, all iron containing compounds.
 * Explanation. Presumably, AN is regulated to control chorismate in response to iron. Little other pathway support for this:
 * nothing in between AN & chorismate pathway is DE.
 * 2 tryptophan dehydrogenases are DE (first step in TRP to kynurenine metabolism), but evidence is contradictory. AN1858 (Afu4g09830) is dn-reg at 6h, same as kynurinases, AN2509 (Afu3g14250) is dn-reg at 4h (when kynurinases are up-reg).

2. oxalic acid
 * little support, intermediate in TCA, TCA cycle linked to iron availability (anaplerotic rxn).


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