User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/08/24

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WiFi coli: A communi-colight system
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Nobody knows it... really O_o
  -H2O ---> 10μl
 * I did a restriction for a total of 20μL as follows to check if the luciferase was ligated in the pBSIIK(+/-):

-Buffer 4 --> 2μl

-BSA ---> 1μl

-DNA --> 5μl

-ECOR1 -> 1μl

-PST1 --> 1μl

  
 * Restrictions were incubated at 37°C ON(overnight) and labeled as LRE No. R-> ECO y PST Mar.
 * Tomorrow, we will repeat the luciferase assay.


 * }