User:Emmalee Jones/Notebook/Lab Notebook/2010/04/08

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Entry title
OK, so now that I am more comfortable with this process next steps are: 1) Make some more flow cells (rinsing after PLL bath) 2) Make some more liposomes - maybe with the mixtures I already made up in buffer, went through freeze/thaw, etc, and test with DLS 3) Try this again, this time with a more dilute mixture of liposomes, like crazy dilute, and see if it still has the uniform fluorescence 4) Fiddle around with that to see at about what concentration I still get uniform coverage 5) Now add MTs back in, try imaging with them again
 * Put liposome solution, same as previous tries except with PEM instead of PEM-T on flow cell marked R
 * Take that cell out of the drawer at 9:13 am, rinse with PEM, seal with nail polish
 * Other flow cell, just rinse with PEM and then seal with nail polish
 * The first slide will be PLL without liposomes
 * Background with just PLL does not seem significant
 * There are bright spots, but they are not bleaching so they are probably just gunk
 * There is a very low level amount on the rhodamine filter, and slightly more brightness all over the flow cell on the FITC filter
 * Now PLL with liposome mixture added
 * There is now basically nothing on the FITC filter - at least nothing I can see
 * On rhodamine there is bright red everywhere (uniform) in the flow cell