The mRNA Decay Resource:Protocols and Resources

Yeast protocols
From the Atkin lab [[Media:RNAextrc.pdf|Total RNA extraction]] Support Protocol: [[Media:rnapheno.pdf|Preparation of buffer saturated phenol]] [[Media:mrnahalf.pdf|Measurement of Saccharomyces cerevisiae mRNA half lives]] Kebaara et al., 2006. Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor. Genome. 49(8):894-9. [[Media:PlyriRNA.pdf|Yeast polyribosome preparation for RNA]] [[Media:RNAgels.pdf|Electrophoresis of RNA through gels containing formaldehyde]] [[Media:NorthRNA.pdf|Northern transfer of RNA]] [[Media:dnaprobe.pdf|Preparation of oligolabeled probes]] [[Media:hybrdRNA.pdf|Hybridization of RNA blots]] [[Media:Remov32P.pdf|Removal of oligolabeled probes from GeneScreen Plus membranes]] [[Media:plyriprot.pdf|Yeast polyribosome preparation - Proteins]] [[Media:protgel.pdf|Gel electrophoresis of proteins in denaturing conditions]] [[Media:protran.pdf|Electrotransfer of proteins from polyacrylamide gels to membranes]] [[Media:imblot.pdf|Immunoblotting]] From the Jacobson lab He et al., 2008. Qualitative and quantitative assessment of the activity of the yeast nonsense-mediated mRNA decay pathway. Methods Enzymol. 449:127-47. From Fred Sherman Sherman, 2002. Getting started with yeast. Methods Enzymol. 350:3-41. From Mike Culbertson Global measurement of mRNA half lives. Guan et al., 2006. Impact of nonsense-mediated mRNA decay on the global expression profile of budding yeast. PLoS Genet. 2(11):e203. Epub 2006 Oct 18. 

General RNA Protocols
Capped RNAs: Bajak and Hagedorn, 2008. Efficient 5' cap-dependent RNA purification: use in identifying and studying subsets of RNA. Methods Mol Biol. 419:147-60. Blewett et al., 2011. A quantitative assay for measuring mRNA decapping by splinted ligation reverse transcription polymerase chain reaction: qSL-RT-PCR. RNA 17(3):535-43. Epub 2011 Jan 10.