Berglund:Spec and Qubit calculators

Spec: DNA (ng/ μL) = Absorbance260nm * dilution * 50 RNA (ng/µl) = Absorbance260nm * dilution * 40 Calc number of molecules: (Absorbance260nm * dilution * 0.05 * 6.02x10^17)/ (MW= 320.5*#nucleotides)= Molecules/μL

Calculation for Qubit: ? (ng/mL) * 100 * (1/1000 mL) * (1 g/10^9 ng) * (10^6 μL/1 L) * (1 mol/32370.5 g) * (10^6 μmol/1 mol) = ? μM ? μM * (1 L/10^6 μL) * (1 mol/10^6 μmol) * 6.02x10^23 = moles/ μL moles/ μL * μL sample = # molecules