IGEM:Imperial/Results/S01656

Sunday, 20 August 2006

 * Normalised Fluorescence of +IPTG +AHL


 * Tube 1 - 233


 * Tube 2 - 237


 * Tube 3 - 239


 * Normalised Fluorescence of +IPTG -AHL


 * Tube 1 - 219


 * Normalised Fluorescence of -IPTG +AHL


 * Tube 1 - 244


 * Normalised Fluroescence of -IPTG -AHL


 * Tube 1 - 218


 * Comparison between +IPTG +AHL and -IPTG +AHL:


 * % Difference = 2.94%
 * Conclusion: Not statistically significant


 * Resolution


 * Test expression of AiiA protein by SDS electrophoresis
 * Re-test the protocol on Tuesday 22 August 2006

Tuesday, 22 August 2006

 * Different formulation used to calculate normalised fluorescence. Used the same calculations as the T9002.  See updated spreadsheet.


 * Normalised Fluorescence of +IPTG +AHL


 * Tube 1 - 4409


 * Tube 2 - 4659


 * Tube 3 - 4574


 * Normalised Fluorescence of +IPTG -AHL


 * Tube 1 - 4574


 * Normalised Fluorescence of -IPTG +AHL


 * Tube 1 - 4769


 * Tube 2 - 4762


 * Tube 3 - 4834


 * Normalised Fluroescence of -IPTG -AHL


 * Tube 1 - 4445


 * Comparison between +IPTG +AHL and -IPTG +AHL:


 * % Difference = 4.25%
 * Conclusion: Not statistically significant
 * Tom 13:58, 26 August 2006 (EDT): Debateable


 * Resolution


 * Test expression of AiiA protein by SDS electrophoresis
 * Re-test the protocol on Wednesday 23 August 2006 with higher concentration of AHL

Results of western Blot


The IPTG has not induced the production of a protein which weighs 28736 kd (aiiA) this should be loacted inside the oval. We are repeating the expperiment to confirm the results. We are sequencing the part to confirm the results. The ladder is invitrogen marker 12.

JohnChattaway 10:13, 23 August 2006 (EDT)

Results of AHL Testing

 * Used 100 stock concentration instead of 10 .  Used 800  of supernatant and 1200  of T9002 cells.


 * Normalised Fluorescence of +IPTG +AHL


 * Tube 1 - 4353


 * Tube 2 - 3552


 * Tube 3 - 4476


 * Normalised Fluorescence of +IPTG -AHL


 * Tube 1 - 4157


 * Normalised Fluorescence of -IPTG +AHL


 * Tube 1 - 3688


 * Tube 2 - 4609


 * Tube 3 - 4197


 * Normalised Fluroescence of -IPTG -AHL


 * Tube 1 - 3608


 * Comparison between +IPTG +AHL and -IPTG +AHL:


 * % Difference = 0.91%
 * Conclusion: Not statistically significant


 * Resolution


 * Re-test the protocol on Thursday 24 August 2006 with same concentration of AHL