IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/13/Zhangs

Double digestion for T7ptag + 1-23L insert Unable to differentiate T7ptag + 1-23L insert and the backbone

Then digest T7ptag+1-23L insert with 3 enzymes System: 20uL 2 samples in totall: T7ptag+T1, T7ptag+T2.

Elcetrophoresis to pure insert. Sample loading order: T7ptag+T1, T7ptag+T2, vector plasmid control, Marker. The insert has been cut out.