User:Fermenter User/Notebook/BROM B9

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM B9

Day 0 (Mar 4 Wed):
           3/4/2009 12:30 Assemble bioreactor             3/4/2009 12:30 Bring media to fermenter room 3/4/2009 12:30 Add 5 ml of Antifoam            3/4/2009 14:30 Start Sterilization (Liq 45 min) 3/4/2009 16:00 Cooling of bioreactor (Temp setup) 3/4/2009 16:30 Purge with 0.5 L/min O2                          3/4/2009 16:59 Start Fermentation Software for Fermenter #6 Record 0:00 3/4/2009 17:01 Start Fermentation Software for Fermenter #7 Record 0:00 3/4/2009 00:00 Calibrate dO2 probe with 300 rpm, O2 purge for 30 min 3/4/2009 17:20 Set dO2 3/4/2009 17:20 Connect Acid (500 ml) and Base (400 ml), prime pumps Adjust pH to 7.0 F6: Base: 400 ml - 240 ml = 160 ml (total consumption) F7: Base: 400 ml - 270 ml = 130 ml (total consumption) 3/4/2009 17:32 Start pH control 3/4/2009 17:32 Completion of bioreactor set up: Ready for inoculation dO2 level is <50%, rpm 400 ---                3/4/2009 19:00 Inoculation: (180 ml) Fermentation 0:00

Day 1 (Mar 5 Thu):
           3/5/2009 (07:00) F6: Acid (470 ml) Base (220 ml) F7: Acid (450 ml) Base (180 ml) Increase rpm to 600 Bioreactor picture taken             3/5/2009 (17:00) Sampling Fermentation 22:00 F6: Cell weight ( 45mg/ml), Activity (74 %, x100), Activity ( 24%, x1000) F7: Cell weight ( 47mg/ml), Activity ( 72%, x100), Activity ( 23%, x1000)            3/5/2009 (17:00) F6: Base + 100 ml (270 ml) F7: Base + 100 ml (250 ml)

Day 2 (Mar 6 Fri):
           3/6/2009 (07:00) F6: Acid (450 ml) Base (270 ml) F7: Acid (360 ml) Base (200 ml)             3/6/2009 (11:00) Sampling Fermentation 40:00 F6: Cell weight (111 mg/ml), Activity ( 91, x100), Activity ( 70, x1000) F7: Cell weight (128 mg/ml), Activity ( 92, x100), Activity ( 67, x1000)             3/6/2009 (18:00) Sampling Fermentation 47:40 F6: Cell weight ( 189mg/ml), Activity ( 90, x100), Activity ( 63, x1000) F7: Cell weight ( 201mg/ml), Activity (90.5, x100), Activity ( 62, x1000)            3/6/2009 (19:00)     Fermentation 48:00 Havest and Finish Fermentation <Sung-Hye>           Finished at      F6: Acid (450 ml) Base (270 ml) F7: Acid (360 ml) Base (200 ml)


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