User:Anthony Salvagno/Notebook/Research/2009/11/25/Unzipping Construct for Doug Smith: pBR322

I don't think I got complete digestion yesterday as there is a feint band above the two known bands. I had to run the gel almost to the end to get good separation. In the future I will need to gel on the larger apparatus. I'll save the purification and the ligation for next Monday so I can show Brian how to do it.
 * 1) 2x Ladder
 * 2) 3x Ladder
 * 3) Digested pBR322