User:Hattie Chung/Notebook/Intron Expression of mStaple/2010/01/19

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title
Have: PCR1.1, PCR2, AgeI-vector
 * Mini-prep pEYFP-2A-Kan from Patrick (Entry vector)
 * PCR 1.1 and PCR 2
 * Digest entry vector with AgeI
 * Make two agarose gel (1%)
 * Run gel, turn on heat block to 53C
 * Purify bands


 * Digest entry vector with NotI
 * PCR 1.2
 * Clean PCR 1.2
 * Digest PCR 1.2 and PCR2 with XhoI


 * }