User:Anthony Salvagno/Notebook/Research/2009/11/17/Annealing Buffer

10x Annealing Buffer
1x Anneal Buffer is: I want to make a 10x stock for easy addition to reactions. My final solution volume will be 500ml, so here is what I need: I have a 100x Concentrate of TE buffer (Tris- EDTA) and 4M NaCl. So here is my recipe:
 * 10mM Tris
 * 1mM EDTA
 * 50mM NaCl
 * 1) 100mM Tris
 * 2) 10mM EDTA
 * 3) 500mM NaCl
 * 4) Rest with H2O
 * 50ml 100x TE
 * 62.5ml 4M NaCl
 * 387.5ml H2O (autoclaved and distilled)