Shreffler:Notebook/retinoic acid trans signaling/2009/07/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Retinoic Acid Trans Signaling
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Neisha A. Rivers
--- Cell count:
 * Transfection

Jurkats = 9.20e5 cells 2 conditions (100nM RA and DMSO): 4.5e5 cells per condition

3T8 = 1.39e6 cells 2 conditions (100nM RA and DMSO) : 4.5e5 cells per condition

--- Addition of Plasmids:

RARE = 2 ug /189.7 ug/ml = 10.5 ul RARE

Pcmv = 2 ug /744 ug/ml = 2.6 ul Pcmv

RXR - 2 concentrations

* 0.1 ug / 3 ug/ml = 0.03 ul RXR * 0.4 ug/ 3 ug/ml = 0.13 ul RXR

RAR - 2 concentrations

* 0.1 ug / 1.1 ug/ml = 0.09 ul RAR * 0.4 ug/ 1.1 ug/ml = 0.36 ul RAR

- Incubation overnight


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