McKinney:7H10

Summary
7H10 agar is selective medium used for the culture of mycobacteria, in particular M. tuberculosis. M. smegmatis is usually grown on LB agar.

Ingredients

 * 19g Middlebrook 7H10 powder
 * deionized water
 * 100mL OADC (Dubos/Middlebrook Oleic Acid Albumin Dextrose Complex)
 * 10mL sterile 50% glycerol

Protocol

 * 1) Mix 19g 7H10 powder in 900mL deionized water in a 2L with a magnetic stirbar until fully dissolved.  Top up to 1L with deionized water in a graduated cylinder, then return to the flask and stir until well mixed.  Cover with aluminum foil and autoclave.
 * 2) Place flasks in a 55&deg;C water bath to cool or cool while stirring with magnetic bar.  Prewarm OADC in 37&deg;C water bath.
 * 3) In a containment hood, add 100mL OADC and 10mL 50% glycerol to the flask.  Replace foil lids, remove from hood, and stir until well mixed.  Pipette 24mL per plate into petri dishes.  Keep flasks in 55&deg;C water bath until ready to pour.
 * 4) Leave plates stacked i nthe hood overnight, then store at 4&deg;C.