Template:SBB10 ConstructionFiles AndrewS sbb12

1) sbb12: Sleeping Beauty 3'TR -- Pool AS001-F through AS008-R, assemble by PCA PCR AS001-F/AS008-R on PCA reaction	(259 bp, EcoRI/BamHI) Substitute in pBjk2741-Bca1144	(EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb12 -- AS001-F PCA Assembly of sbb12 CCATAGAATTCATGAGATCTTTGAGTGTATGTTAACTTCTGACCCACTGG AS002-R  PCA Assembly of sbb12	ATTTCAGCTTTTATTTCTTTCATCACATTCCCAGTGGGTCAGAAGTTAAC AS003-F  PCA Assembly of sbb12	AAAGAAATAAAAGCTGAAATGAATCATTCTCTCTACTATTATTCTGATAT AS004-R  PCA Assembly of sbb12	AGGATCACCACTTTATTTTAAGAATGTGAAATATCAGAATAATAGTAGAG AS005-F  PCA Assembly of sbb12	TAAAATAAAGTGGTGATCCTAACTGACCTTAAGACAGGGAATCTTTACTC AS006-R  PCA Assembly of sbb12 CACTTTTTCACAATTCCTGACATTTAATCCGAGTAAAGATTCCCTGTCTT AS007-F  PCA Assembly of sbb12	TCAGGAATTGTGAAAAAGTGAGTTTAATGTATTTGGCTAAGGTGTATGTA AS008-R  PCA Assembly of sbb12	CTGATGGATCCCAGTTGAAGTCGGAAGTTTACATACACCTTAGCCAAAT



JCA Notes

 * correct
 * Need to put parts in clotho