User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/14

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 * style="background-color: #EEE"|[[Image:Penn_fulllogo.gif|128px]] Caspase-3 Activatable Probe
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Miniprep
with Qiagen Kit

Digestion

 * EcoRI & SgrAI (.5 μL each)
 * 10 μL colony DNA (or 1 μg control plasmid)
 * NEB Buffer 4

Gel Electrophoresis

 * Naming Scheme:
 * G = hgLuc, R = hRLuc8
 * 1 = 1xDEVD, 5 = 5xDEVD
 * Final Number = Colony Number



Results
It didn't look like anything cut, so I probably messed up the restriction digest... I sent the first two colonies in for sequencing anyway, since the previous screen gave high yield.


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