Registry/Measurement kit/Notebook/2007-7-26

Plates

 * No growth on I2056.
 * F2620-3K3 sample 1 and F2620-1AK3 have a few small colonies. F2620-3K3 sample 2 has 1 colony

To Do

 * PCR clean digests from yesterday
 * Ligate them
 * Mini-Prep and digest 3K3 (E/X)

ccdB

 * PCR products are digested and cleaned up.

I2055

 * Ran gel of PCR, made o/n of only colony that looked right size.

T9002

 * Running pcr of F2620-3k3 and 1AK3

BB PCRs

 * Primers came today, so started E0240 (E-S) and I2057 (E-X) (Tm=52 deg)

Ligations
F2620-1AK3
 * I2055-1AK3 and 3K3
 * I2056-1AK3 and 3K3
 * I2057-1AK3
 * E0240-1AK3

I2055 Synthesis

 * Decided not to make the silent point mutation; will use an AatII site on 3K3 (~100bp upstream of the cloning site) instead of MfeI