SBB09 Construct36

Construction of  basic part

PCR Oph022-F/Oph023-R on F plasmid (398 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10036 {a~TraA>} - Oph022-F       foward oligo for cloning of {a~TraA>} ccaaaGAATTCatgAGATCTacttatgaatgctgttttaagtgttcaggg Oph023-R       reverse oligo for removal of stop codon {a~TraA>} gcaaaGGATCCgaggccaacgacggccatac