User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/01

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04/01/10

 * &#x2713; Cultures: KAH96 rebuilt (4); KAH014 (8)
 * &#x2713; Minipreps: KAH014(DPRE) (4)
 * &#x2713; Cultures: KAH014 (8)
 * &#x2713; Sequencing: KAH014; KAH144, 145 (confirmation of EED expression cassettes)

Minipreps > Check with E/P digests

--> KAH014-1 and 2 might be good (77 bp band?). Send for sequencing. --> Pick 8 more clones from colony plate.

Sequencing (Genewiz, standard)
 * 1) KAH014-1 P0001: bad (w.t. PstI #1)
 * 2) KAH014-1 P0002: good (mut PstI #2)
 * 3) KAH014-2 P0001: bad (w.t. PstI #1)
 * 4) KAH014-2 P0002: good (mut PstI #2)
 * 5) KAH144 P0001: good
 * 6) KAH144 P0002: good
 * 7) KAH145 P0001: good
 * 8) KAH145 P0002: good


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