User:Matthew Whiteside/Notebook/Fumigatus Microarray/2009/03/18

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Iron effect on metabolism in fumigatus
Hypothesis: changes in iron modulates citric acid cycle, in the following ways (based on SreA study)


 * 1) Direct repression of anaplerotic rxn enzymes & transporters through SreA (anaplerotic rxn replenish TCA intermediates that are used in biosynthesis rxn). Genes regulated by SreA, related to anaplerotic rxns are:
 * 2) *GABA permease 5g00710 (part of cluster that is down-reg in Fe-replete conditions)
 * 3) *NAD-dependent formate dehydrogenase 6g04920
 * 4) *Beta-IPM (isopropylmalate) dehydrogenase 6g13590 (oxalic intermediate)
 * 5) Direct repression of glycogen & alt sugar utilization. Genes regulated by SreA (caution: little supporting evidence):
 * 6) *Glycogen phosphorylase 1g12920
 * 7) *L-arabitol dehydrogenase 3g01490
 * 8) Indirect repression of anaplerotic rxn enzymes & transporters. Genes repressed during iron-replete conditions:
 * 9) *GABA:
 * 10) **3g07150
 * 11) Indirect repression of anaplerotic rxn enzymes & transporters. Genes up-regulated during iron-replete conditions (some withincreased up-reg with intact SreA*):
 * 12) *GABA:
 * 13) **4g08170*
 * 14) **5g06680*
 * 15) *Oxalic acid:
 * 16) **7g01010*
 * 17) **2g03820
 * 18) Indirect action on glycogen & alt sugar utilization (didn't go into details)

There appears to be complex transcriptional control mediated by iron conditions. In a few cases, SreA appears to directly repress some genes.


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