User:DavidRamos/Lab/2006-6-20

Morning

 * Dive-Into-Python Chapters 2-5
 * Functions, Datatypes, List functions, Classes and Objects
 * Make glycerol stocks of R0010+E0241
 * 100uL 50% glycerol, 100uL liquid media
 * DNA Miniprep
 * Follow handout; had 2 5mL samples, only used one of them
 * Tubes 0.5mL PCR; labeled in blue on top
 * Digest for diagnosis
 * Xba1 and Pst1
 * .5uL Xba1, .5uL Pst1
 * 11uL h20
 * 2.5uL 10X BSA
 * 2.5uL #3 NebBuffer
 * 8uL DNA
 * Incubate @ 37C for 1h

Afternoon

 * Ran DNA on an E-gel. Lane7 in the picture below:
 * Further brainstorming on DNA nanostructure design.
 * Considered flat-sheet and honeycomb-lattice versions of each of the following structures: cylinder, tetrahedron, cube.
 * Eventually decided to go with honeycomb-lattice cylinder since we have already made those.
 * Considered multiple ideas for DNA nanotube lids. Measurements and calculations will be posted tomorrow.
 * Peng did some research and found that we need to set up some equipment to raise cyanobacteria. And by set up, I mean build.  Home Depot road trip on the morrow?  Stay tuned!