User:Fermenter User/Notebook/BROM B10

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM B10

Day 0 (Aug 17 Mon):
           8/17/2009 10:30 Assemble bioreactor             8/17/2009 11:00 Bring media to fermenter room            8/17/2009 11:25 Start Sterilization (Liq 45 min) 8/17/2009 13:00 Add 5 ml of (Sterilized) Antifoam 8/17/2009 13:30 Cooling of bioreactor (Temp setup) 8/17/2009 13:30 Purge with 1.0 L/min O2                          8/17/2009 13:39 Start Fermentation Software for Fermenter #6 Record 0:00 8/17/2009 13:40 Start Fermentation Software for Fermenter #7 Record 0:00 8/17/2009 14:00 Connect Acid (250 ml) and Base (400 ml), prime pumps Adjust pH to 7.0 F6: Base: 400 ml - 240 ml = 160 ml (total consumption) F7: Base: 400 ml - 240 ml = 160 ml (total consumption) 8/17/2009 14:00 Start pH control 8/17/2009 14:10 Calibrate dO2 probe with 400 rpm, O2 purge for 30 min 8/17/2009 14:10 Set dO2 8/17/2009 14:15 Completion of bioreactor set up: Ready for inoculation 8/17/2009 15:30 dO2 level is <50%, rpm changed to 350 ---                8/17/2009 18:00 Inoculation: (180 ml) Fermentation 0:00

Day 1 (Aug 18 Tue):
           8/18/2009 (09:00) Fermentation 15:00 F6: Acid (200 ml) Base (130 ml) F7: Acid (220 ml) Base (120 ml) Increase rpm to 600 Bioreactor picture taken             8/18/2009 (11:00) Sampling Fermentation 17:00 F6: Cell weight ( 45mg/ml), Activity (76 %, x100), Activity ( 10%, x1000) F7: Cell weight ( 47mg/ml), Activity ( 69%, x100), Activity ( 16%, x1000)            8/18/2009 (13:30) Fermentation 19:30 Changed filter of F7 (pressurized)

Day 2 (Aug 19 Wed):
           8/19/2009 (09:00) Fermentation 39:00 F6: Acid (170 ml) Base (20 ml) F7: Acid (200 ml) Base (30 ml)             8/19/2009 (10:00) Sampling Fermentation 40:00 F6: Cell weight (93 mg/ml), Activity ( 95, x100), Activity ( 65, x1000) F7: Cell weight (82 mg/ml), Activity ( 94, x100), Activity ( 66, x1000)             8/19/2009 (15:00) Sampling Fermentation 45:00 F6: Cell weight ( 95mg/ml), Activity ( 93, x100), Activity ( 66, x1000) F7: Cell weight ( 80mg/ml), Activity (92, x100), Activity ( 65, x1000)    8/19/2009 (15:30) Fermentation 45:30 Harvest F6: Acid (160 ml) Base (20 ml) F7: Acid (200 ml) Base (30 ml)


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