IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/06/Zhangs

Identify the RBS+CI+T ligation product by electrophoresis Result (from left to right) Marker, RBS plasmid control, 1-5J+CI+T①~⑤ (plasmid), 1-2I+CI+T①~⑤ (plasmid), 1-11N+CI+T①~⑤ (plasmid) The ligation of 1-5J+CI+T failed Marker, RBS plasmid control, 1-1H+CI+T①~⑤ (plasmid), 1-2G+CI+T①~⑤ (plasmid) The 1-1H+CI+T1, 5 & 1-2G+CI+T1, 2, 3, 5 are the target plasmids. Marker, RBS plasmid control, 1-5N+CI+T①~⑤ (plasmid) The 1-5N+CI+T2, 4, 5 are the target plasmids.

Choose samples: 4μL 1H+CI+T⑤, 4μL 2G+CI+T⑤, 6μL 5N+CI+T⑤, 6μL 2I+CI+T⑤, 6μL 11N+CI+T④ Concentration 217.5ng/μL     233ng/μL       125ng/μL       162ng/μL       123ng/μL

Double-digestion of CI+1-23L insert (causing the ligation of 1-5J+CI+T failed). 4 hours digesting. Electrophoresis to purify the CI+1-23L insert, and extraction.