User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/19

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

03/19/10

 * &#x2713; Minipreps: KAH142, 96/V0201 (2 each)
 * &#x2713; Sequencing order: KAH96/V0201
 * &#x2713; Cultures: KAH142/V0201 (8)

Minipreps > Check with XbaI digest > XbaI fragment will have an extra 641 bp from vector sequence; remaining vector = 4516

--> Sequence KAH96/V0201 plasmids --> Pick 8 new colonies for KAH146/V0201

Sequencing order


 * 1) KAH96_V0201-1 3T3: HSVtkTATA-Kozak is missing
 * 2) KAH96_V0201-1 BGHR: good
 * 3) KAH96_V0201-2 3T3: HSVtkTATA-Kozak is missing
 * 4) KAH96_V0201-2 BGHR: good

--> Minimal promoter is missing! KAH142 might also be bad. Sequence construct intermediates KAH86 and 96 in V0120.


 * }