User:Karmella Haynes/Notebook/Polycomb project/2010/02/17

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

02/17/10

 * &#x2713; Polycomb-ATF RT-PCR cultures: split plates
 * &#x2713; Pc-ATF lines: split confluent plates (KAH128-8, 132-8) 1:10 in 10 cm dishes
 * &#x2713; Protein prep: Pc-ATF lines (+Dox, 2 days)

RNA prep/ RT-PCR cultures > Dox induction so far: 2 days > Split plates: split plates by 1/2 (double number of plates) > Add dox (final = 1 μg/mL) to Dox+ samples


 * }