User:Vijay/Notebook/2007-2-14

Fractionation

 * 1) Cell fractionation. Done
 * 2) *200ml culture used
 * 3) *OD at 560nm - 27 (using PBS)
 * 4) *200ul lysostaphin used to lyse cells
 * 5) *40000g for membrane separation, using Type 70Ti
 * 6) **Acel, Decel - both 7
 * 7) **Temp 4oC
 * 8) **Time 1hr