User:Kalkao/notebook/Making Overnight Suspensions


 * 1) Retrieve streaked plates the 30*C incubation room
 * 2) *If colonies need more time to form, place in the 37*C incubation room for faster growth
 * 3) *Plates with colonies can be kept in the 4*C fridge until for awhile
 * 4) Place your plates on the lab bench
 * 5) Obtain one test tube for each plate streaked
 * 6) Sterilize a pipette:
 * 7) *Using a fireboy, flame the top of the pipette
 * 8) *Place the pipet into the pipetteman
 * 9) *Now, slowly flame the entire length of the pipette
 * 10) Pipette 5mL of T-broth into a test tube
 * 11) *Make sure the cap is on the T-broth bottle when it is not in use
 * 12) *Flame the opening of the T-broth bottle directly before and after the pipette enters the bottle
 * 13) *Flame the opening of the test tube before and after the T-broth is added
 * 14) *Dispose of the pipette in the bleach water
 * 15) Use the sterile loop to move a single colony from one plate to one test tube with T-broth
 * 16) *Carefully touch one colony with the sterile loop
 * 17) *Put sterile loop into the T-broth in the test tube
 * 18) *Use the loop to mix the T-broth
 * 19) *Vortex the test tube
 * 20) *Flame the sterile loop before it touches the agar plate and after it leaves the test tube
 * 21) *Remember to flame the entire length of the sterile loop for this step because the much of the wire will be inside the test tube when mixing the T-broth
 * 22) *Flame the opening of the test tube directly before and after the sterile loop is inside
 * 23) *Repeat this step for every streaked plate
 * 24) Bring the test tube cultures to the 37*C incubation room
 * 25) Stop the rotating tube rack
 * 26) Place test tube with cell cultures on the tube rack
 * 27) *Make sure the rack is balanced
 * 28) Turn the rotor back on and set the dial between 7 and 8
 * 29) Parafilm the streaked plates and place in the 4*C fridge