Endy:Screening plasmid/v2.0/Construction/Queue

This page is to keep up with the constructions that are in progress. When a part is completed be sure to add it to the glycerol box. To find out what these parts are please read testing or construction areas.

=Todo list=

=To be designed=

=Completed (seq-verified)=
 * I13545 (GFP.ins_site.mRFP1)
 * I13546 (F2620 + GFP.ins.mRFP1) -- 1360-1498 (missing ~10 bp in here)
 * between 1350 and 1450 there are 11 N’s. accoding to the chromatogram each one is most likely correct.
 * I13547 (F2621 + GFP.ins.mRFP1)
 * 14 N’s between 1440 and 1510. Most of them checked out OK, some ambiguous.
 * I13549 (F2620 + mRFP1)
 * I13550 (F2621 + mRFP1)
 * I13551 (F2620 + ins.mRFP1)
 * I13552 (F2621 + ins.mRFP1)
 * I13553 (PenI + GFP/RFP)
 * I13554 (F2620 + GFP)
 * I13555 (F2621 + GFP)
 * I13558 (Ptet expressing G/hairpin/RFP)
 * I13559 (F2620.CFP)-- middle unsequenced

=Awaiting sequencing results= =Length-verified=
 * I13561 - looks (much) too short, checking sequence
 * I13562 - looks (a little) too short, checking sequence

=To be digested=

=To be prepped=

=Planning=
 * I13556 - F2620 + mCherry
 * I13557 - F2621 + mCherry
 * I13560 - F2621 + CFP
 * I13562 - polycistronic mCherry/GFP
 * I13561 - polycistronic mRFP1/GFP
 * The above two don't contain BB sites, they are just control constructs

To move into MG1655

 * I13546 - F2620+GFP/RFP (need to prep more dna)
 * I13547 - F2621+GFP/RFP (need ot prep more dna)