Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/07

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7 May 2009 Lab Meeting
† Announcements † Flu R01 † SEPSIS Project †RNA project † COBRA - Making substrate in microwewlls? (motivation? need to implement valves) - Co-evaporate colloids? (the morphology won't be uniform or consistent each time, signal analysis system non-existent) - Sol-gel on Teflon? (can serve to study basic material science only) - Do Rhodamine. Leaked. Need to try again. - Found the problem. Liquid and air channel widths reversed. Will make new chip and try again. -Need a size specific positive control. PS beads? charged? † Virus concentration
 * New lab meeting time T 10a-12pm room 705 starting 5/12/2009
 * Progress report done.
 * sample collection done..
 * Updates?
 * Three flu fixtures completed today at the machine shop for 400$, another one ordered for general purpose use (Jane and Jaephil)
 * Three flu fixtures completed today at the machine shop for 400$, another one ordered for general purpose use (Jane and Jaephil)
 * Revision in process due 5/20
 * Jeff visiting week of May 25th
 * Integration with the glass slide.
 * new air channel design sent to machine shop
 * new fluid channel made in PDMS
 * making the polyurethane mold had proven challenging as it sticks to the plastic fixture, tried mold release but didn't help
 * new cutting plotter carrier sheet ordered
 * new air channel mold did not cure well and the PDMS made from it was leaking. need to do it again, mold material ordered.
 * Substrates (JZ)
 * Let's get this to a point where we can publish and move on....JZ and CMK to discuss.
 * Colloid experiment
 * 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
 * try coated PS beads.
 * ATCC flu virus grew and harvested
 * Plaque assay of harvested virus in progress: learning from Brendan, will try if enough cells
 * Concentrator chip design underway

† Valve Array/Valve building - done. † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR
 * Who is in charge of this now that the SP students are gone? Alex?
 * Paper in revision Yeah!
 * Bonding meeting with MIT folks - Alex left some samples with them. Updates?
 * Final draft with collaborators.
 * designing herringbone groove for better trapping (Mask writer down until next Tuesday)
 * investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
 * IRB still in revision.
 * Cathie read and gave back PCR 1 paper to QQ and MCK
 * Qingqing has written the introduction
 * repeated test of the PCR efficiency for different designs of channel.
 * test PCR efficiency using smaller DNA size (63bp product).
 * analyze PCR product for different temperature using bioanalyzer.
 * getting statistical data for the activation energy from 50 particle simulation (Fig. 5-7)
 * Mincheol has written the introduction, results and discussion, and conclusion.
 * will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)

† RCA/HDA † Senior project
 * Giving up on white paper. Think about other grant outlets.
 * Preliminary no-valve design for 25 microliters.
 * 20 more test chips to Sonali (AACC poster).
 * Sonali try to do 10 microliters in the tube * Updates?
 * Jane is working on passive valve characterization: threshold pressure not related to cantilever length or bonding time, try hole size next
 * Megan
 * HDA on chip. Make simple chip that mixes two things for HDA. Move chip to heater, do HDA demonstration = home run.
 * Plasma helped bonding.
 * EHS wants MSDS stuff.

† Silica Optimization (Lambda):


 * Run single PCR product from QQ or Sonali the test with the bioanalyzer


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