User:Linh N Le/Notebook/2009/08/27

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To Do

 * We are running low on tips and tubes, so I need to autoclave some today and tomorrow before I leave

Autoclaving

 * Doing the rest of the yellow tips in the red boxes
 * about 8 in total
 * Also showing Brian how to use the Autoclave

Things that I dont want to fall into obscurity

 * Koch mentioned that I should make sure any work I have done does not get forgotten
 * I'm pretty sure I have documented all the work I've done
 * Here's a list of things that are useful to the lab that I may not want to die:
 * Inventory Stuff
 * Profiling laser beams? (it is a bit of an archaic method, but it works on a budget)
 * The laser stuff is all on the private wiki, might be a good idea to have it transferred to the public
 * My Comic (on the private wiki). Anyone is allowed to put new comics in, they dont even have to follow the "stick figure" approach is use.
 * I have a few ideas that I may need to draw in retroactively, i've just been too busy/lazy to do them lately

Inventory'n

 * All the inventory stuff is located at http://www.openwetware.org/wiki/Koch_Lab:Inventory
 * I even wrote a little blurb at the bottom of this page on how to inventory (which I just updated with more specific details)

Tubulin

 * Andy, Brigette and I resuspended some Rhodamine Tubulin today (made 4 aliquots) 1ul in ea aliquot (5mg/ml)
 * We then took 1 aliquot of RT and then added it to an aliquot of unlabeled tubulin
 * We have 6, 1ul aliquots that are 15% RT
 * I went ahead and polymerized one of the mixed aliquots and we created motility soln
 * Andy tried a motility assay, but it failed
 * Brigette and I forgot to add Dextrose to the motility soln and the MT's became "kinked"
 * Retrying with dextrose now added
 * ''Note: The 15% RT is enough to see, which is good
 * Success! We got to see motility and the MT's are really long and easy to see
 * They are moving quite fast and the AF is working great
 * No camera (in tweezers lab) so no photo/video footage. Hopefully it works again tomorrow and Andy can bring over the cam
 * Andy Maloney 23:22, 27 August 2009 (EDT): I'm glad to hear it worked. Did you guys change out the ATP to get it to work or did you use the same stuff?
 * Steve Koch 23:48, 27 August 2009 (EDT): Awesome! Glad to hear about motility.  I think we'll have a camera soon?
 * Linh N Le 02:30, 28 August 2009 (EDT) We added the dextrose and that fixed all the problems (brigette did most of the work, her notebook will state if she did anything else differently)


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