IGEM:Penn State University 2006/calendar/2006-7-5

=Wednesday=

To do today
*e-mail Randy about R0051 and E. coli strain *make sequencing primers (gene-walking and reverse primers) *help Lane with mini-preps, restrictions, gel purification, etc.- assemble LaqIq + motB construct

Accomplished from yesterday
*Sequencing files obtained, examined

To do today

 * restrict (4h) IIR, E0422, E0432, E0434 and run gel
 * resubmit primers and IIR+motB and motB for sequencing
 * annoy Lane by not inquiring about 96 well plate

Accomplished from yesterday

 * miniprep P0451 (from 2005 plate), IIR (from cold room plate), and R0010.motB.B0015 (from successful ligation)

To do today
*

Accomplished from yesterday
*

To do today
*inoculate at 11 AM for plate reading at 7PM. Plate reading for 24 hour culture.

Accomplished from yesterday
*

To do today
*

Accomplished from yesterday
*

To do today
*mini-prep LacIq constructs *restrict, ligate, transform (LacIq, B0015+MotB) *innoculate Mary's quorum constructs *do sequencing stuff

Accomplished from yesterday
*Restricted, gel purified, ligated, transformed Mary's GFP+AAV tagged quorum constructs