Kubke Lab:Research/CND/Records/RC013

=Embryo details=

Species: Gallus gallus domesticus Embryo Name: RC013 Embryo stage: ST30 Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. (Notebook entry 10th January 2011). Staging has not been confirmed. Fixation: PFA Cryoprotection: None. Material label and storage: Stored in vial filled with PFA labeled RC-013 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
 * Shape of beak matches ST30.
 * There is a distinct dip in the hindbrain's curve which is present on ST29-ST30 embryos.
 * The forebrain is split along the midline running laong the rostro-caudal axis.
 * There are no dots in the eye only faint pigmentation around the perimeter (<ST31).
 * Morphology of the toes matches 30 more closely than 29.

=Experiment details= Objective:To use as a practice embryo for staging. Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage. Comments:

=Results=

Embryo is approximately ST30.

=Images= =Summary=

This embryo may be used in further experiments as approximately a ST30 embryo however confirmation is needed.