Tk:protein tags


 * References
 * PMID 12238768
 * Current Protcols in Protein Science, section 9.9


 * protein purification tags, antibody epitopes
 * FLAG
 * DYKDDDDK, pmid 8770418
 * FLAG Epitope description
 * c-MYC
 * EQKLISEEDL
 * HA
 * YPYDVPDYA
 * 6HIS
 * HHHHHH
 * Strep
 * AWRHPQFGG
 * Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
 * GST (n-terminal)
 * Chitin binding
 * BCCP biotin carboxyl carrier protein
 * VSVG
 * Thrombin cleavage domain
 * LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
 * Calmodulin tag
 * S-tag
 * GFP protein tags
 * T7 epitope
 * MASMTGGQQMG (N terminal or internal)
 * Novagen T7 tag purification kit (Novagen), pGEMEX vectors (Promega)
 * Makrides 1996
 * V5 tag
 * GKPIPNPLLGLDST (C-terminal)
 * pET, pBAD and Gateway (Invitrogen)
 * McLean 2001


 * protein solubility
 * maltose binding protein
 * Thioredoxin


 * Protein cleavage sites
 * Enterokinase
 * Asp-Asp-Asp-Asp-Lys↓
 * some side cutting at basic residues
 * Factor Xa
 * Ile-Glu/Asp-Gly-Arg↓
 * Thrombin
 * Leu-Val-Pro-Arg↓Gly-Ser
 * TEV protease
 * Glu-Asn-Leu-Tyr-Phe-Gln↓Gly
 * PreScission
 * Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro