AB medium

Purpose

 * AB medium is a minimal growth medium used for bacterial cultures. It has some changes compared to M9 medium and is supposed to cause less problems with precipitation Clark-JMB-1967.

Recipe
Prepare and autoclave.

Mix 200ml 5xA and 800ml B to get 1 liter AB. Do never add B salts to 5xA!

If you want agar-plates add 16g agar to 1xB, autoclave and than add 5xA.

Different things can or should be added:


 * 1) Carbon source (has to be added):
 * 2) 0.2% glucose (10ml of 20% or 4ml of 50% sterile stock)
 * 3) 0.4% acetate (20ml of 20% sterile sodium-acetate stock)
 * 4) Amino acids (check AA requirement of your strain):
 * 5) 0.5% casamino acids (50ml of 10% sterile stock)
 * 6) For CM735 add 20 μg/ml methionine (10 ml of 2mg/ml stock), 20 μg/ml tryptophane (5 ml of 4mg/ml stock) and 25 μg/ml histidine (1 ml of 25 mg/ml stock);
 * 7) 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)
 * 8) 25μg/ml uridine (0.25 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine Jensen-JB-1993 .)

Contact

 * Please feel free to edit this page, add comments on the talk page or contact the page beginner Torsten Waldminghaus.