IGEM:Harvard/2010/Plasmids

the plant vectors we will be biobrick-ing and using to transform into arabidopsis come from this paper:

Coutu, et al. "pORE: a modular binary vector series suited for both monocot and dicot plant transformation" Transgenic Res (2007) 16: 771-781.

this paper established a whole slew of vectors with different plant promoters and antibiotic resistance markers.

we have ordered the following vectors: O1, O2, E3, E4, R1, and R3.

these plasmids currently contain multiple cloning sites (MCSs) with many, many restriction enzyme cut sites. we will need to change the MCSs such that they are biobrick compatible. [[Media:pORE_O1.gb|pORE_O1]] [[Media:pORE_O2.gb|pORE_O2]] [[Media:PORE_E3.gb|pORE_E3]] [[Media:PORE_E4.gb|pORE_E4]] [[Media:PORE_R1.gb|pORE_R1]] [[Media:PORE_R3.gb|pORE_R3]]

[[Media:V7.gb|V7: pORE_O1 biobricked]] [[Media:V8.gb|V8: pORE_O2 biobricked]] [[Media:V9.gb|V9: pORE_E3 biobricked]] [[Media:V10.gb|V10: pORE_E4 biobricked]] [[Media:V11.gb|V11: pORE_R1 biobricked]] [[Media:V12.gb|V12: pORE_R3 biobricked]]