IGEM:MIT/2005/Friday 8/1 Meeting Agenda

Input

 * We don't know if the fluor. oligos/molecules are even entering the cell
 * Check on mic. settings
 * Check for autofluor?
 * Controling the pH: fluor. is an indicator
 * Maybe electroporation isn't the best idea
 * Lets clean up our technique so we know how to test our system
 * Lets talk to the advisors -- need some new plans

Head Receiver

 * For the ToxR system, do we need TT?

ToxR

 * atcc -- order primers
 * start assemblies if parts arrive

FecA

 * Lab:
 * Making FecA', quickchange
 * coordinating for making parts
 * Ordering primers for fusion, and fecA promoter
 * Gathering materials for transformation
 * Xgal and IPTG for QuikChange
 * Knockouts: getting constructs -- ASAP

Signal Processing

 * An experiment will happen if assemblies arrive