Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18/7.24.08 Gel Results

=7.24.08 Colony PCR Results=

100 volts, 30 minutes, 1% Agarose gel. 5 &mu;l of product in each lane. Stained for 15 minutes. Everything except lane 7 (corresponding to colony 6 of the pKn004.L3 transformation) yielded a product at what I think is ~500 bp. Colony 6 is at what I think to be slightly above the ~1500 band, where I expect it assuming a proper integration.