Endy:Screening plasmid/Constructs

Version 0

 * Version 0.1
 * I13512 - PBad RFP GFP Term
 * I13513 - PBad RFP Insertion GFP Term
 * I13514 - PBad GFP Insertion RFP Term


 * Version 0.2
 * I13529 - RFP upstream, GFP downstream, one RNAseE site in between.
 * I13530 - GFP upstream, RFP downstream, one RNAseE site in between.
 * I13533 - One promoter. Insertion site has flanking RNAseE sites. GFP is downstream and has a 5' hairpin.
 * I13534 - One promoter. Insertion site has flanking RNAseE sites. RFP is downstream and has a 5' hairpin.


 * Version 0.3
 * I13537 - Two promoters. I13458 split out, I13453 drives both GFP and RFP.
 * I13538 - Two promoters. I0500 drives GFP, I13453 drives RFP
 * I13539 - Two promoters. I0500 drives RFP, I13453 drives GFP

Version 1

 * Version 1.0
 * Version 0.2 - I13534 was released as Screening Plasmid v1.0 (pSB1A10) on the MIT Registry of Biological Parts following promising results in screening/characterization of terminators and inverters.
 * Design - One promoter. Insertion site has flanking RNAseE sites. RFP is downstream and has a 5' hairpin.

Controls

 * I13543 - Pbad driving RNAseE/hairpin/RFP - inducible promoter
 * I13522 - constitutive GFP
 * I13521 - constitutive RFP
 * I13540 - inducible GFP
 * I13520 - inducible RFP
 * V1023 - negative (CW2553/pJAt8)