IGEM:MIT/2006/Notebook/2007-1-19

Results from Yesterday's Transformations
osmYFull.E0840

Passed antibiotic resistance test- only grows on AMP/TET

J45800 hopefuls

All grew

osmYShort.inverter.E0840

Some colonies

J45170.J45320

Very few colonies- Maybe 3

Course of Action
1. Colony PCR J45170.J45320s and J45800 hopefuls- DONE

2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840- DONE

3. LC J45170.J45320, J45800, osmYShort.inverter.E0840, osmYFFull.E0840- DONE

4. Make plates- DONE

5. Check sequencing (results will not be available until later tonight)

6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE)

7. Smell IK cells- SMELL GREAT TO ME- DONE

8. Update google doc- DONE

9. Make a list of needed submissions and resubmissions to the Registry- DONE

List of Needed Submissions to the Registry
J45120

Cells in the Registry have a mix of plasmids. We isolated a colony with just J45120 and have a glycerol available.

J45180

The J45180 in the Registry is merely osmY + inverter - R0040. We have another glycerol containing the correct J45180, osmYQ119B.

J45700 & J45900 (Constitutive Banana)

The J45700 in the Registry is merely J45320. After an antibiotic test and an analysis using the GC, we can submit these parts.

J45600 & J45800

We are in the process of building/verifying.

J45995, J45996, J45997, & J45998

Cells glyceroled and verified via sequencing and antibiotic test

J45999

We have a glycerol of these in our fridge. The entry in the Registry most likely contains a plasmid since it is chloramphenicol AND ampicillin resistant.