User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/01

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] SDS-PAGE for immunoblot (fail)
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Gel #2 was tried with fresh APS 10% (previous was 1 month old)
 * Still large blur was seen during and after running the gel.
 * When checking pH of the stacking gel a pH of 7.15 was found instead of 6.8
 * This will prevent the stacking function and would explain the blur seen while running
 * New stacking buffer was prepared
 * 18.9 g TRIS
 * 150 mL dH2O
 * pH 6.8
 * added to 250 mL with dH2O
 * Gel #3 was prepared
 * All other gels stored at 4 °C were discarded
 * To prevent more wasting of samples only marker and loading buffer (4x) was used
 * seemed to run owkay after also running buffer was renewed
 * Immunotreatment of blot #2 was performed as described yesterday

Method

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