IGEM:PennState/Labbook/GalenLynch/2007-7-9

July 9th, 2007: 9:30-6:15 Results from over the weekend: Initial pfx pcr failed, second attempt was a success. Restrtiction, extraction, purification, ligation, and transformation was performed on the successful bands. Restriction results were verified by gel, with discernable bands. Despite this, transformations were unsuccessful. Transformations Today's plans: Repeat ligations and transformations.
 * New vial of T4 ligase purchased
 * Ligations: all in PSB1A2 (from death gene BBa_P1010)
 * BBa_I741005 XylR Transcriptional Regulator
 * BBa_I741011 XylR Generator
 * BBa_I741015 Xyl Promoter
 * BBa_I741017 Xyl Promoter Reverse Compliment
 * BBa_I741018 XylF Promoter
 * BBa_I741019 XylA Promoter
 * BBa_I741020 XylF Truncated Promoter (No CRP Site)
 * Duplicate of BBa_I741015
 * Puc19 positive control
 * PSB1A2 negative control

All ligations were performed for both ten minutes and an hour, producing two sets of plates. Meeting