Silver: Lysate for Western

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 * 1) Grow cells
 * 2) Harvest
 * 3) *Spin down
 * 4) *Wash with PBS
 * 5) *Spin down enough cells to collect a 50-100 µL cell pellet in a FastPrep tube
 * 6) Prepare lysis buffer (beforehand) and chill on ice
 * 7) *PBSMT:
 * 8) **2.5 mM MgCl2
 * 9) **3 mM KCl
 * 10) **0.5% Triton X-100
 * 11) **in PBS
 * 12) *Add protease inhibitors
 * 13) **PMSF: 1 mL per 100 mL buffer
 * 14) ***Use fresh PMSF: dissolve 0.035g per 1mL 100%EtOH
 * 15) **PLAAC: 100 µL per 100 mL buffer
 * 16) **0.5 M Benzamidine: 260 µL per 100 mL buffer
 * 17) Add 50-100 µL of cold lysis buffer to cell pellet and vortex
 * 18) Add 1-2 scoops glass beads (bead level should be just below liquid level)
 * 19) Mix everything together by vortex
 * 20) Lyse in FastPrep bead beater
 * 21) *6.5 m/s
 * 22) *40 second lysis
 * 23) Add 500 µL lysis buffer (keep everything on ice!)
 * 24) Spin down at maximum speed in tabletop centrifuge for 10 min. (in cold room)
 * 25) Remove supernatant (lysate) to another tube.