User:Nzimm

Bio

 * MIT Class of 2009
 * Major: Biological Engineering
 * Minors: Management and Music
 * Varsity Soccer
 * Wind Ensemble (Clarinet)

M13K07 Genome Refactoring:
In refactoring the M13K07 genome between the HpaI site in gene II and the BamHI site in gene III, I separated all overlapping elements across the region by copying overlapping sequences and placing the elements adjacent to one another on the refactored sequence. As a result, the refactored region is 1,000 bp larger than the original sequence. I also added annotation for & refactored the open reading frames of genes 2, 5, 7, and 10, which are not annotated in the M1307 part in the registry. I inserted the sequence encoding the myc protein into g3. I intend to separate each element with two unique restriction sites, so that the elements can be isolated & manipulated more easily. I initially proposed to add a fluorescent tag like GFP to g5, but seeing as how GFP is nearly 3 times the size of the protein product of g5, I chose to leave g5 unaltered. Below is a table describing the overlaps that were refactored.

Current Courses

 * 20.109
 * 7.05
 * 14.01
 * 21M.301

Other
Email: nzimm (at) mit (dot) edu

SAGA subunits, S. cerevisiae
20.109:Module 4 Research Proposal