Van Oudenaarden Lab:C17C3.10

Progress:

Probes designed: (03-04-2010 - Christoph Engert)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

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>C17C3.10 (spliced + UTR - 966) ATGGACCGCA GAAAGCTTCA AAAAGAGGCA AGAAGTGAAA TGAAAAAGAC CGCCAATGGC GTCGACCGTG CCACATATAA CCGAGCAAAG GCTCTTTTGG AGATAATCCG GAAGGAAGAG ATTGCCACAA ATGATCTGGC TTCCTCCACG ATCTCCAAGA TGCCAAAAGT TATCCCATCT TCAATGAGCG ATTATCGTTC CGTCCCATAC AATCAAACTC CAAAATCAGG ATCTGAACGG AAAAGAAGAA ATATTACAAA CGAGTTGATT AACGAATGCA AGACGATTGT TCAAAAATCA GAAGAAGAAC ATATATCACA AGAAGTAGTT CTCTTCCGAA TTGTGAAACT TGTGACTGGA GTTAATCTGG AATCTAATTT CTCATCGAAT GACCTCTCCG AATCTACAAG ACGGAAGTTT GATACTGAAT CCGAAAGAAG AAAAGTGAAG ACTGAACGTG AAAAGATTCG AAGAAAGAAA CAAGATGACT GTTATGCTGA ATTGAAATTC TTCATTTTGA ACAAACAAAT GGGAAGTTAC GAGCAACGAT TAAAACTTGA ACGGATCACA ATTCTAGAAA TCATCATTGA TTACATTAAA CACAATTCCG ATCTTCTGTA TCCAGAAACA ATTCCCCAAA TACTTCCTCT TTTAGCTGGA AAATCAACTG CTACTTGTGA GAACAAAGAG AATGAAAAGC CAAAAACACG AATGGAAGTG AAGGATCTCT TCCCAAGATT GACGTTTCAA GAAGTTCAAG AATCCCCAAC ATCTACTTCT CCATTGCTCA CATTTCCATG TATTCCAATG ATCCCAACTA CTCAGTTCAA TGTCTTGTCA AACTACAACA CCGTTCCATC AATATTTTCT GCTCCCCTCC GATTCATTCT TCCATCACTT CAAATCTTAA CACCAGAAAC TAGTGACGAG GAAGAAAATG AAGAAACCGT CGATATTAGT AACTAG

Name of probe set: hlh-27 Probes designed on Wed, 03 Mar 10 23:45:39 -0700 40 probes designed for target of length 966

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

ttttgaagctttctgcggtc, hlh-27_1, 1, 4, 45 ggcggtctttttcatttcac, hlh-27_2, 2, 35, 45 ttatatgtggcacggtcgac, hlh-27_3, 3, 61, 50 ctccaaaagagcctttgctc, hlh-27_4, 4, 83, 50 caatctcttccttccggatt, hlh-27_5, 5, 105, 45 gaggaagccagatcatttgt, hlh-27_6, 6, 127, 45 ttttggcatcttggagatcg, hlh-27_7, 7, 149, 45 cgctcattgaagatgggata, hlh-27_8, 8, 171, 45 ttgtatgggacggaacgata, hlh-27_9, 9, 193, 45 cagatcctgattttggagtt, hlh-27_10, 10, 216, 40 gtaatatttcttcttttccg, hlh-27_11, 11, 238, 30 gcattcgttaatcaactcgt, hlh-27_12, 12, 260, 40 ctgatttttgaacaatcgtc, hlh-27_13, 13, 282, 35 cggaagagaactacttcttg, hlh-27_14, 14, 319, 45 ctccagtcacaagtttcaca, hlh-27_15, 15, 342, 45 gaggtcattcgatgagaaat, hlh-27_16, 16, 377, 40 acttccgtcttgtagattcg, hlh-27_17, 17, 399, 45 cttctttcggattcagtatc, hlh-27_18, 18, 421, 40 ttcacgttcagtcttcactt, hlh-27_19, 19, 443, 40 cttgtttctttcttcgaatc, hlh-27_20, 20, 465, 35 ttcaattcagcataacagtc, hlh-27_21, 21, 487, 35 cttcccatttgtttgttcaa, hlh-27_22, 22, 517, 35 caagttttaatcgttgctcg, hlh-27_23, 23, 540, 40 atttctagaattgtgatccg, hlh-27_24, 24, 562, 35 cagaagatcggaattgtgtt, hlh-27_25, 25, 599, 40 tttggggaattgtttctgga, hlh-27_26, 26, 621, 40 tttccagctaaaagaggaag, hlh-27_27, 27, 643, 40 gttctcacaagtagcagttg, hlh-27_28, 28, 665, 45 gtttttggcttttcattctc, hlh-27_29, 29, 688, 35 gagatccttcacttccattc, hlh-27_30, 30, 710, 45 cttgaaacgtcaatcttggg, hlh-27_31, 31, 732, 45 gatgttggggattcttgaac, hlh-27_32, 32, 754, 45 aaatgtgagcaatggagaag, hlh-27_33, 33, 776, 40 gttgggatcattggaataca, hlh-27_34, 34, 799, 40 tgacaagacattgaactgag, hlh-27_35, 35, 821, 40 ttgatggaacggtgttgtag, hlh-27_36, 36, 843, 45 aatcggaggggagcagaaaa, hlh-27_37, 37, 865, 50 gatttgaagtgatggaagaa, hlh-27_38, 38, 887, 35 cgtcactagtttctggtgtt, hlh-27_39, 39, 909, 45 cgacggtttcttcattttct, hlh-27_40, 40, 933, 40 -- Input sequence with probe locations:

atggaccgcagaaagcttcaaaaagaggcaagaagtgaaatgaaaaagaccgccaatggcgtcgaccgtg ctggcgtctttcgaagtttt          cactttactttttctggcgg      cagctggcac Probe # 1, 45% GC             Probe # 2, 45% GC         Probe # 3,

ccacatataaccgagcaaaggctcttttggagataatccggaaggaagagattgccacaaatgatctggc ggtgtatatt ctcgtttccgagaaaacctc  ttaggccttccttctctaac  tgtttactagaccg 50% GC    Probe # 4, 50% GC     Probe # 5, 45% GC     Probe # 6, 45%

ttcctccacgatctccaagatgccaaaagttatcccatcttcaatgagcgattatcgttccgtcccatac aaggag gctagaggttctacggtttt  atagggtagaagttactcgc  atagcaaggcagggtatg GC    Probe # 7, 45% GC     Probe # 8, 45% GC     Probe # 9, 45% GC

aatcaaactccaaaatcaggatctgaacggaaaagaagaaatattacaaacgagttgattaacgaatgca tt  ttgaggttttagtcctagac  gccttttcttctttataatg  tgctcaactaattgcttacg Probe # 10, 40% GC   Probe # 11, 30% GC    Probe # 12, 40% GC

agacgattgttcaaaaatcagaagaagaacatatatcacaagaagtagttctcttccgaattgtgaaact ctgctaacaagtttttagtc                gttcttcatcaagagaaggc   acactttga Probe # 13, 35% GC                  Probe # 14, 45% GC     Probe # 1

tgtgactggagttaatctggaatctaatttctcatcgaatgacctctccgaatctacaagacggaagttt acactgacctc              taaagagtagcttactggag  gcttagatgttctgccttca 5, 45% GC                Probe # 16, 40% GC    Probe # 17, 45% GC

gatactgaatccgaaagaagaaaagtgaagactgaacgtgaaaagattcgaagaaagaaacaagatgact ctatgacttaggctttcttc ttcacttctgacttgcactt  ctaagcttctttctttgttc  ctga Probe # 18, 40% GC   Probe # 19, 40% GC    Probe # 20, 35% GC    Prob

gttatgctgaattgaaattcttcattttgaacaaacaaatgggaagttacgagcaacgattaaaacttga caatacgacttaactt         aacttgtttgtttacccttc   gctcgttgctaattttgaac e # 21, 35% GC           Probe # 22, 35% GC     Probe # 23, 40% GC

acggatcacaattctagaaatcatcattgattacattaaacacaattccgatcttctgtatccagaaaca gcctagtgttaagatcttta                ttgtgttaaggctagaagac  aggtctttgt Probe # 24, 35% GC                  Probe # 25, 40% GC    Probe # 26

attccccaaatacttcctcttttagctggaaaatcaactgctacttgtgagaacaaagagaatgaaaagc taaggggttt gaaggagaaaatcgaccttt  gttgacgatgaacactcttg   ctcttacttttcg , 40% GC   Probe # 27, 40% GC    Probe # 28, 45% GC     Probe # 29, 3

caaaaacacgaatggaagtgaaggatctcttcccaagattgacgtttcaagaagttcaagaatccccaac gtttttg cttaccttcacttcctagag  gggttctaactgcaaagttc  caagttcttaggggttg 5% GC   Probe # 30, 45% GC    Probe # 31, 45% GC    Probe # 32, 45% G

atctacttctccattgctcacatttccatgtattccaatgatcccaactactcagttcaatgtcttgtca tag gaagaggtaacgagtgtaaa   acataaggttactagggttg  gagtcaagttacagaacagt C   Probe # 33, 40% GC     Probe # 34, 40% GC    Probe # 35, 40% GC

aactacaacaccgttccatcaatattttctgctcccctccgattcattcttccatcacttcaaatcttaa gatgttgtggcaaggtagtt aaaagacgaggggaggctaa  aagaaggtagtgaagtttag  tt  Probe # 36, 45% GC    Probe # 37, 50% GC    Probe # 38, 35% GC    Pr

caccagaaactagtgacgaggaagaaaatgaagaaaccgtcgatattagtaactag gtggtctttgatcactgc   tcttttacttctttggcagc obe # 39, 45% GC     Probe # 40, 40% GC