IGEM:Harvard/2006/Salt-ethanol precipitation

Note: this protocol is preliminary

Small-scale separation using salt:ethanol precipitation
Stock is now concentrated 2x, but we probably suffered some losses. Run a gel and check spot-density of the bands to determine yield.
 * Add 0.25 mass equiv of 5 M NaCl (assume 1.19 g/mL density)
 * example: 100 * 0.25 / 1.19 = 21 
 * Add 0.5 mass equiv of ethanol (assume density of 0.79 g/mL)
 * example: 100 * 0.5 / 0.79 = 63.3 
 * Incubate on ice 10 min
 * Centrifuge at 15000 rpm for 10 min at 4°C
 * Carefully remove supernatant with pipette
 * Resuspend in 50 1x folding buffer