WangLab:Simple Purification of Adenovirus

Simple purification of adenovirus

 * 1) 50 ul to DMEM 10 ml
 * 2) Put into preconfluent 293 cells.
 * 3) 24 hr later, see green
 * 4) 48 hr later, floating cells.
 * 5) Collect cell+sup
 * 6) Freeze-thaw 3 times (no warming)
 * 7) Centrifuge 1000g
 * 8) Get supernatant
 * 9) Aliquote and store in -70.
 * 10) 100-200 ul to 10 ml DMEM.