IGEM:Cambridge/2008/Notebook/Voltage/2008/08/11

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Kdp?

 * PCR colonies on plates - no insert seen.
 * Transform overweekend ligation.

OsmY and RBS

 * PCR B0030 and restrict 10μL with Xba.
 * Plasmid extract OsmY and restriction digest with Xba.
 * Ligate and transform cells with 5μL


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