Chang Lab:Notebook/CBE/08/146/2008/12/17

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title
Time start:830am


 * Cultured bacteria (for biofilm optimization trial III next day)
 * Single loop taken from streaked petri dishes on 13/12/08 and suspended into 10ml broth in a test tube (incubate at 37 C for 24hrs).

Note
 * Plates incubated on 15/12: Sonicated biofilms from Trial I
 * No growth observed for 10^-6, 10^-8, 10^-10 dilutions; need to reduce dilutions for Trial II plating.
 * Plates incubated on 16/12/08: Biofilm Inoculum (matched at 1.0 McFarland)
 * LB: Too many to count at 10^-10, 10^-12, 10^-14; need to increase dilutions for Trial II plating
 * M9: No growth at 10^-10, 10^-12, 10^-14 (questionable fault with the centrifuge procedure).


 * }