IGEM:Harvard/2006/Fusion proteins/thrombstrepadapt


 * Oligos (8 ssDNA sequences):

Strep + 5

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAG 3'

Strep +20:

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTC 3'

Strep +35:

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTCACTTCTGCAGCAATA 3'

Strep +50:

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTCACTTCTGCAGCAATAGAATTAAGCTTGTCC 3'

Thromb + 5:

5' GGTTGGTGTGGTTGGACACT 3'

Thromb + 20:

5' GGTTGGTGTGGTTGGACACTGAACCTCTAGATGCT 3'

Thromb + 35:

5' GGTTGGTGTGGTTGGACACTTATTGCTGCAGAAGTGAACCTCTAGATGCT 3'

Thromb + 50:

5' GGTTGGTGTGGTTGGACACTGGACAAGCTTAATTCTATTGCTGCAGAAGTGAACCTCTAGATGCT 3'


 * Alignment

+20: 5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTC                       3' 3'                                             TCGTAGATCTCCAAGTCACAGGTTGGTGTGGTTGG    5' +35: 5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTCACTTCTGCAGCAATA 				3' 3'                                             TCGTAGATCTCCAAGTGAAGACGTCGTTATTCACAGGTTGGTGTGGTTGG      5' +50: 5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTCACTTCTGCAGCAATAGAATTAAGCTTGTCC			 3' 3'                                             TCGTAGATCTCCAAGTGAAGACGTCGTTATCTTAATTCGAACAGGTCACAGGTTGGTGTGGTTGG 5'