User:Anthony Salvagno/Notebook/Research/2009/11/17/Ligating New Constructs

Setup 1

 * 30' - added 3ul more
 * 60' - added 3ul more
 * 90' - added 3ul more

Setup 2
I kinda messed up in that I added 2.5ul of Adapter insead of the 2ul I had planned. This isn't a huge deal since it allows me to put in more than what is needed.
 * 30' - added 2.5ul more
 * 60' - added 2.5ul more
 * 90' - added 2.5ul more

Next Time
Tomorrow I will run a gel of the results and pray to the biology gods that there is successful ligation.