User:Tara K. Luckau/Notebook/Team ConGen/2011/01/14

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Gel Lance conditions
Success!!!


 * [[Image:20110114_Full2.TIF|800 px]]


 * [[Image:20110114_Scun2.jpg|600 px]]
 * Scun2 Primer - expecting bands ~150bp
 * Scun sample - band at 130bp
 * Scoc sample - band at 75bp


 * [[Image:20110114_Scun22.TIF|600 px]]
 * Scun22 Primer - expecting bands at ~450bp
 * Scun sample - multiple bands; but there is one prominent band in one of the replicates at 450bp
 * Scoc sample - multiple bands; but prominent band in all three replicates at 450bp

Differences between Lance conditions and previous trials
The largest difference between the two reactions was Taq - 10-fold difference


 * In the interest of cost, try to pull this down to 1 Unit/reaction (0.2µL)
 * Also re-calculate for 10µL reaction volume
 * Find annealing temperatures (touchdowns are poopy)

PCR decrease Taq

 * [[Image:20110114_PCROpt_Touchdown.jpg|900 px]]