Template:SBB10 ConstructionFiles BenB sbb05

Construction of sbb05: piggieBac 5'TR

Pool BDBpb001 through BDBpb010, assemble by PCA PCR BDBpb001/BDBpb010 on PCA reaction	(344 bp, EcoRI/BamHI) Sub in pBjk2741-Bca1144	(EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb05

BDBpb001	PCA assembly of sbb11	CCATAGAATTCATGAGATCTTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGC BDBpb002	PCA assembly of sbb11	GCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATC BDBpb003	PCA assembly of sbb11	GAAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTGTGCATTTAGG BDBpb004	PCA assembly of sbb11	CTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCACAGCGACGGA BDBpb005	PCA assembly of sbb11	CGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCACTGATTTTGA BDBpb006	PCA assembly of sbb11	CGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTACCGC BDBpb007	PCA assembly of sbb11	CCGCGTGAGTCAAAATGACGCATGATTATCTTTTACGTGACTTTTAAGATTTAACTCATACG BDBpb008	PCA assembly of sbb11	TATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGT BDBpb009	PCA assembly of sbb11	ATATTGTTATTTCATGTTCTACTTACGTGATAACTTATTATATATATATTTTCTTGTTATAGATATCGGATC BDBpb010	PCA assembly of sbb11	CTGATGGATCCGATATCTATAACAAGAAAATATATATATAATAA



JCA Notes

 * Correct