User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/20

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Nuclear Gene HS Takara PCR optimization
All amps failed - repeat with 2.0ul Primer concentration as control

load 2.oul of sample



Cycle Sequence HCO/LCO PCR Product

 * 07April10 Ethanol Cleaned PCR Product - added 20ul more water = total 30ul -> used this for CS

InstaGene Matrix
load 2.5ul of sample



QIAamp DNA mini extraction kit
load 2.5ul of sample



PCR

 * standard long PCR conditions