User:Karmella Haynes/Notebook/Polycomb project/2011/04/08

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04/08/11

 * &#x2713; cDNA synthesis: Pc-TF RNA samples from 4/07/11

cDNA Synthesis

> oligo(dT) Primer annealing --> Do 4 rxn's for 129-4 #1 --> 2 rxn's ea. for 129-4 +dox #4 & #5

> cDNA synthesis mix --> 16 reactions total

--> Add 10 μL mix to each annealing rxn. --> 50°C/ 50 min., 80°C/ 5 min., ice --> Add 0.8 μL RNase H, 37°C/ 20 min. --> Store at -20°C


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