Endy:Making a long term stock of bacteria

''Back to Making a long term stock of bacteria

Materials

 * 40% glycerol solution
 * Cryogenic vials

Method

 * Add 1 ml of 40% glycerol in H2O to a cryogenic vial.
 * Add 1 ml sample from the culture of bacteria to be stored.
 * Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
 * Alternatively, pipet to mix.
 * Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
 * On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
 * Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.