Template:SBB10 ConstructionFiles JennaK sbb28

Biobricking of CA42 rep gene basic part sbb28

PCR repCA42-F and repCA42-R on pEC22-CA42 gene     (1496 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144                          (EcoRI/BamHI, 918+2472, L) Product is pBca9523-repCA42			     {5'UTR.repCA42!} repCA42-F  Cloning of repCA42   ccaaaGAATTCtaaAGATCTcagctgaagtgaccggattag repCA42-R  Cloning of repCA42   gctagGGATCCctattttccgcttttccagtttcg



JCA Notes
Biobricking of CA42 rep gene basic part sbb28
 * Correct except prefix should be ATG, not TAA
 * Size of vector dig slightly off
 * Reverse oligo can be shared with another part
 * Name of plasmid should be partName, not partDescription

PCR repCA42-F and KRM002 on pEC22-CA42 gene     (1496 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144                          (EcoRI/BamHI, 910+2472, L) Product is pBca9523-sbb28     {5'UTR.repCA42!} repCA42-F  Cloning of repCA42 ccaaaGAATTCatgAGATCTcagctgaagtgaccggattag KRM002  Reverse oligo for cloning of rbs.repCA42! ctgatGGATCCctattttccgcttttccag