BioMicroCenter:Protocols

BioMicro Center Protocol Testing

 * RT-PCR Protocol for Sample QC
 * Cluster Visualization Protocol for Flowcell QC

ILLUMINA SEQUENCING PROTOCOLS
To access the official Illumina protocols please got to the Illumina website and create an iCom account. Once an iCom account has been created please feel free to use the links below to download specific protocols.

[[Media: BioMicro_Single_Read_Illumina_Primer_Seq_WorkFlow.xlsx | Illumina Single Read and Multiplexing Sequence Workflow]]

 Cluster Station and Genome Analyzer 
 * Cluster Station Operations Guide
 * Cluster Station User Guide
 * Genome Analyzer II User Guide

 ChIP-Seq 
 * ChIP-Seq Sample Prep Guide

 mRNA-Seq 
 * Directional RNA-Seq Library Prep Pre-Release
 * DSN Normalization Sample Prep Guide
 * mRNA-Seq Sample Prep Guide

 SmallRNA-Seq 
 * SmallRNAv1.5 Sample Prep Guide
 * SmallRNA Oligo Only Kit Sample Prep Guide

 Genomic DNA </BIG></B>
 * Genomic Sample Prep
 * Paired End Sample Prep Guide
 * Multiplexing Sample Prep Guide
 * Mate Pair 2-5Kb Sample Prep Guide

AGILENT MICROARRAY PROTOCOLS

 * aRNA Synthesis and Labeling - [[Media:aRNA_labeling.docx| DOWNLOAD]]
 * Hybridization - [[Media:hybridization.docx| DOWNLOAD]]
 * Wash and Scan - [[Media:wash_scan.docx| DOWNLOAD]]

Caliper Information

 * BioRad HSP dimensions - [[Media:BioRad HSP dimensions Feb 2011.pdf| DOWNLOAD]]