User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/27

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Stimulation cover slips, PKA-Co-IP
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Immunofluorescence of cells

 * Cells for immunofluorescence measurement were stimulated with 15% or with serum free DMEM (S0)

PKA Co-IP

 * All samples used before for AKAP250 Co-IP were diluted 10x in ddH2O
 * 1 mL of each sample was incubated >1 h with PKA Iα/β reg (H-90): sc-28893 @ 4°C while rotating


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