Godin:Protocols/Microscopes

Abstract
Measure thickness and lateral size of sample with the inverted microscope

Equipment
Microscope : Olympus BX41 Microscope camera : Cool snap HQ2

Procedure
For thickness (Z): - Focus on top of your sample. - Read the graduation on the fine adjustment knob. - Focus on the bottom of your sample. - Read the graduation on the fine adjustment knob. - Subtract two readings. - Multiply this number by 1.2 to have your thickness in micrometer approximately.

For lateral size (X,Y): - Take a picture of your sample. - For 10x50x → 1pixel≈129.7μm - For 10x20x → 1pixel≈325.7μm