Van Oudenaarden Lab:C17C3.8

Progress:

Probes designed: (03-04-2010 - Christoph Engert)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

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>C17C3.8 (spliced + UTR - 755) ATGTCTTCCT CTCCAACTTC GTCATCATCT GGGTCCCCTT CTTCACATGG ACATCGATCT GAAACAGAAA AACAAAGAAG AGATGATACT AATGATCTGT TGAATGAATT CAAGAAAATC GTTCAAAAAT CGGAATCTGA AAAACTTTCA AAAGAAGAGG TTCTATTTCG TATTGTGAAA TTGTTATCTG GTATTCAACT TCATCACGAA TCGTTTTCAA CATCGCCTGG ACCAATTAGA AGTATTAAAA AGATTAAAAG CGATCGGGAA CAAGTTCGCC GAAATAAGAG AGTTGCCGCG TACAGAGAGC TCAGAAAATT TATTGCATTG AATAATTTGA GTAGCTCAGA AGAAATAGAC AAAATGGAGA ATTTGAAGGT TCTAGAAATC ATCTTTGAAG TGATTCGTGG AAAGTCGATC ACATGTACAG TACCTTGCCT ACCATTTCCA ATACTTCCTT TTCTCCCAGT ATTCCCAGTT TATTCGCCAT TAGTTTTCAA TTCATACAAT CAATTTTCAC TTTATCCTCC TCACATGCCT ACAGTTCAGA TTCCTATTGT CCCAAGTTCC AGCTTCGAAA TTGACGCTCT GGAAATAGAA GAAAATGAGA AAGATATCGA TATTGTTGGC TGAagagatt cagctgaaaa tcgtattggt ctccaatttt tttcaacagt tttcaacatt atttagacat cttttgcatc ccgattcatt gaaaattaat aaatttaaaa aagtaacatc aagat

Name of probe set: hlh-26 Probes designed on Wed, 03 Mar 10 23:47:03 -0700 30 probes designed for target of length 755

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

gaagttggagaggaagacat, hlh-26_1, 1, 1, 45 aagaaggggacccagatgat, hlh-26_2, 2, 24, 50 gtttcagatcgatgtccatg, hlh-26_3, 3, 46, 45 cagatcattagtatcatctc, hlh-26_4, 4, 80, 35 cgattttcttgaattcattc, hlh-26_5, 5, 102, 30 gaaagtttttcagattccga, hlh-26_6, 6, 130, 35 acgaaatagaacctcttctt, hlh-26_7, 7, 152, 35 gaagttgaataccagataac, hlh-26_8, 8, 183, 35 gatgttgaaaacgattcgtg, hlh-26_9, 9, 205, 40 aatacttctaattggtccag, hlh-26_10, 10, 227, 35 gttcccgatcgcttttaatc, hlh-26_11, 11, 252, 45 actctcttatttcggcgaac, hlh-26_12, 12, 274, 45 ttttctgagctctctgtacg, hlh-26_13, 13, 299, 45 ctgagctactcaaattattc, hlh-26_14, 14, 330, 35 ttctccattttgtctatttc, hlh-26_15, 15, 352, 30 gatgatttctagaaccttca, hlh-26_16, 16, 374, 35 gactttccacgaatcacttc, hlh-26_17, 17, 397, 45 gcaaggtactgtacatgtga, hlh-26_18, 18, 419, 45 aaggaagtattggaaatggt, hlh-26_19, 19, 441, 35 taaactgggaatactgggag, hlh-26_20, 20, 463, 45 tgaattgaaaactaatggcg, hlh-26_21, 21, 485, 35 gtgaggaggataaagtgaaa, hlh-26_22, 22, 515, 40 taggaatctgaactgtaggc, hlh-26_23, 23, 537, 45 ttcgaagctggaacttggga, hlh-26_24, 24, 560, 50 cttctatttccagagcgtca, hlh-26_25, 25, 582, 45 cagccaacaatatcgatatc, hlh-26_26, 26, 613, 40 acgattttcagctgaatctc, hlh-26_27, 27, 635, 40 gttgaaaaaaattggagacc, hlh-26_28, 28, 658, 35 gatgtctaaataatgttgaa, hlh-26_29, 29, 682, 25 ttcaatgaatcgggatgcaa, hlh-26_30, 30, 704, 40 -- Input sequence with probe locations:

atgtcttcctctccaacttcgtcatcatctgggtccccttcttcacatggacatcgatctgaaacagaaa tacagaaggagaggttgaag  tagtagacccaggggaagaa  gtacctgtagctagactttg Probe # 1, 45% GC     Probe # 2, 50% GC     Probe # 3, 45% GC

aacaaagaagagatgatactaatgatctgttgaatgaattcaagaaaatcgttcaaaaatcggaatctga ctctactatgattactagac cttacttaagttcttttagc        agccttagact Probe # 4, 35% GC    Probe # 5, 30% GC           Probe # 6,

aaaactttcaaaagaagaggttctatttcgtattgtgaaattgttatctggtattcaacttcatcacgaa ttttgaaag ttcttctccaagataaagca           caatagaccataagttgaag  gtgctt 35% GC    Probe # 7, 35% GC              Probe # 8, 35% GC     Probe

tcgttttcaacatcgcctggaccaattagaagtattaaaaagattaaaagcgatcgggaacaagttcgcc agcaaaagttgtag gacctggttaatcttcataa     ctaattttcgctagcccttg  caagcgg
 * 9, 40% GC    Probe # 10, 35% GC       Probe # 11, 45% GC    Probe #

gaaataagagagttgccgcgtacagagagctcagaaaatttattgcattgaataatttgagtagctcaga ctttattctctca    gcatgtctctcgagtctttt           cttattaaactcatcgagtc 12, 45% GC      Probe # 13, 45% GC             Probe # 14, 35% GC

agaaatagacaaaatggagaatttgaaggttctagaaatcatctttgaagtgattcgtggaaagtcgatc ctttatctgttttacctctt acttccaagatctttagtag   cttcactaagcacctttcag  ag Probe # 15, 30% GC    Probe # 16, 35% GC     Probe # 17, 45% GC    Pr

acatgtacagtaccttgcctaccatttccaatacttccttttctcccagtattcccagtttattcgccat tgtacatgtcatggaacg tggtaaaggttatgaaggaa  gagggtcataagggtcaaat  gcggta obe # 18, 45% GC   Probe # 19, 35% GC    Probe # 20, 45% GC    Probe

tagttttcaattcatacaatcaattttcactttatcctcctcacatgcctacagttcagattcctattgt atcaaaagttaagt         aaagtgaaataggaggagtg  cggatgtcaagtctaaggat   a
 * 21, 35% GC           Probe # 22, 40% GC    Probe # 23, 45% GC     P

cccaagttccagcttcgaaattgacgctctggaaatagaagaaaatgagaaagatatcgatattgttggc gggttcaaggtcgaagctt actgcgagacctttatcttc           ctatagctataacaaccg robe # 24, 50% GC   Probe # 25, 45% GC             Probe # 26, 40% GC

tgaagagattcagctgaaaatcgtattggtctccaatttttttcaacagttttcaacattatttagacat ac ctctaagtcgacttttagca   ccagaggttaaaaaaagttg    aagttgtaataaatctgta Probe # 27, 40% GC    Probe # 28, 35% GC      Probe # 29, 25% GC

cttttgcatcccgattcattgaaaattaataaatttaaaaaagtaacatcaagat g aacgtagggctaagtaactt Probe # 30, 40% GC