User:Xiao Y. Liu/Notebook/BioE140L/2010/02/07

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oligo design for sbb03: tos site of N15 using geneDesign

 * 1 biolding block, length of building block is 550 bp,
 * average Tm of oligo overlaps in building block is 46°,
 * number of oligos in building block: 20,
 * average oligo length: 47bp, longest oligo: 54bp, shorest oligo: 28bp.

Construction file for tos (check for errors)
1) sbb03: tos site of N15

Pool tos01 through tos20, assemble by PCA PCR tos01/tos20 on PCA reaction	(534bp,EcoR1/BamH1, gp) Sub into pBca9145-jtk2625   (EcoRI/BamHI, 2057+929bp, L) Product is pBca9145-Bca1144

tos01	PCA assembly of tos CCATAGAATTCATGAGATCTTCTAAGCGCAACGGTATTACTTACGT tos02	PCA assembly of tos ATTTAAAATCATTAAGTTAGGTTTTAAATATACCAACGTAAGTAATACCGTTGC tos03	PCA assembly of tos TTAAAACCTAACTTAATGATTTTAAATGATAATAAATCATACCAATTGC tos04	PCA assembly of tos CAGCATGTTCGCTTAACTTTTGATAGCAATTGGTATGATTTATTATC tos05	PCA assembly of tos AAAGTTAAGCGAACATGCTGATTTTCACGCTGTTTATACACTTTGAG tos06	PCA assembly of tos ATATAGAGACGGAAGAGATAGAGATGCCTCAAAGTGTATAAACAGCG tos07	PCA assembly of tos TATCTCTTCCGTCTCTATATTGAAACACAATCAAAGAACATCAATCC tos08	PCA assembly of tos TTCTTAGATAGTGGGGGATGTCACATGGATTGATGTTCTTTGATTG tos09	PCA assembly of tos ATCCCCCACTATCTAAGAACACCATAACAGAACACAACATAGGAATG tos10	PCA assembly of tos TGTTCCGAATTATTGATACATTAATGTTGCATTCCTATGTTGTGTTCTG tos11	PCA assembly of tos AATGTATCAATAATTCGGAACATATGCACTATATCATATCTCAATTACGG tos12	PCA assembly of tos ATGGGCAATTGTGTGCTGATATGTTCCGTAATTGAGATATGATATAG tos13	PCA assembly of tos TCAGCACACAATTGCCCATTATACGCGCGTATAATGGACTATTGTGTG tos14	PCA assembly of tos GTTCTGCGCTTATGTTCTCCTTATCAGCACACAATAGTCCATTATAC tos15	PCA assembly of tos GAGAACATAAGCGCAGAACAATATGTATCTATTCCGGTGTTGTGTTC tos16	PCA assembly of tos TATAAGAGAACATAATAGCAGAATAACAAAGGAACACAACACCGGAATAG tos17	PCA assembly of tos TCTGCTATTATGTTCTCTTATAGTGTGACGAAAGCAGCATAATTAATC tos18	PCA assembly of tos ATATCGTAACACAATCAAAGAACAAGTGACGATTAATTATGCTGCTTTCG tos19	PCA assembly of tos TTCTTTGATTGTGTTACGATATCCAGAGACTTAGAAACGGGGGAAGG tos20	PCA assembly of tos CATAGGGATCCTTCCCCCGTTTCTAAGT


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