User:Meng Xiao He/Notebook/fall08/2008/11/23

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Conjugation continued

 * Filter approach
 * filters vortexed (syringes and seals still attached) > LB DAP passed thru > LB Gm sucked thru filter into syringe > vortex again > fluid in syringe centrifuged > supernatant removed > wash with LB twice > cells plated on LB Gm and used to inoculate LB Gm liquid culture
 * filters wiped with EtOH externally, placed entirely within LB Gm to shake at 30°C
 * Centrifuge approach
 * cells pelleted > supernatant removed > washed with LB twice > streaked onto and spread onto LB Gm, used to inoculate LB Gm liquid culture


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