User:Dileep D. Monie/Notebook/BBF Standards Measurement/2008/04/01

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] BBF Standards: Promoter Measurement Test
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

2008-04-01

 * Added 5ml of Supplemented M9 Media containing 20 μg/ml Kanamycin (Sigma K0254; lot no. 027K2378) to fifteen 17mm test tubes
 * Added 5ml of Supplemented M9 Media with no antibiotic (for the TOP10 negative control cells) to three 17mm test tubes
 * Inoculated media by picking single colonies from plates streaked on 2008-03-24 (each strain was inoculated in triplicate from different colonies)
 * Grew the cultures at 37°C with shaking at 70 RPM


 * }