PNK Treatment of DNA Ends protocol

Solutions/reagents:  PNK stock  (10, 000 U/ml) T4 ligase buffersubstrate Equipment: IncubatorReaction tubes Steps:   Reaction Mix (10 µl)  Use the following table as a checklist for preparing the reaction in reaction tube (1): </li>   Reaction Conditions  Incubate at <font color=#357EC7>37°C  for <font color=#357EC7>30 mins . Incubate at <font color=#357EC7>65°C  for <font color=#357EC7>20 mins . Store the tube <font color=#357EC7>on ice . </li> </ol> TOTAL TIME REQUIRED FOR THE COMPLETION OF THE PROTOCOL :<font color=#357EC7>~ 50 mins