User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/21

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Check Transformation
Expecting zero colonies for pSB1A3 E|P and multiple colonies from BBa_J23100

Digestion
BBa_I13504 will be a downstream part, and thus digested with XbaI and PstI

Ligation
Incubate for 2 hours in room temperature
 * 1) 7ul BBa_I13504 X|P
 * 2) 7ul BBa_katE E|S
 * 3) 1ul pSB1A3 E|P

Transformation
Ligated part

Overnight growth
BBa_J23100 (test promoter)


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