IGEM:metu/2009/Notebook/wound dressing/2009/08/03

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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03.08.2009
1. 15:10 --- Transformation

EGF / BBa_Q04400_ By Caitlin Conbay_ 2009 plate 1-16 P EGF /  BBa_B0015 By Reshma Shetty- 2009 plate 1- 23L KGF / BBa_F1610_ 2009 plate 2-24 G KGF /  BBa_R0011- 2009 plate 1- 6 G

2. 17:00 --- measurement of nanodrop

3. 18.00 --- In order to make plasmid isolation, we put 10 ml LB+Amp + Single transformed backbone colony for 16 hours incubation.

4. 18:00 – DIGESTION

Double digestion Backbone    prefix: EcorI & suffix : PstI = Buffer O Lysis        XbaI and PstI = 1x tango, 2 fold PstI (procedure : fermentas)