IGEM:IMPERIAL/2007/Notebook/2007-9-14

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/12 view=threemonths format=%name/%year-%month-%day weekstart=7

Restriction Digest of Biobricks

 * 1) 4 DNA samples were digested by SpeI and PstI
 * 2) * Samples 5, 7, 24
 * 3) 1 DNA sample wasdigested by XbaI and PstI
 * 4) * Samples 23
 * 5) 4 DNA samples were digested by EcoRI
 * 6) * Samples 13, 25, 27b-e, T7, Kirsten's parts
 * 7) 4 μl of DNA was digested by 1 μl of enzymes at 37&deg;C for 1 hour Exception: Sample 23 had 6 μl DNA and 2 μl enzymes

Protocols can be found at Restriction Digest in the general protocols page

Agarose Gel Electrophoresis of Biobricks

 * 1) Checked 9 Biobricks and 13 Digests on 1% agarose gel

Conclusions:
 * Part 27 is confirmed to be faulty
 * T7 maxi-prep was not done properly
 * Kirsten's parts were most likely not there...

Protocols can be found at Electrophoresis in the general protocols page

Gel Purification of Biobricks

 * 1) Gel was melted and run through column
 * 2) Purified DNA was eluted with ddH2O
 * 5. BBa_R0040 [ptet] (~2100 bp fragment)
 * 7. BBa_F2620 [ptet-LuxR-pLux] (~3100 bp fragment)
 * 23. BBa_I13504 [GFP] (~800 bp fragment)
 * 24. BBa_R0062 [pLux] (~2100 bp fragment)

Protocols can be found at Gel Purification in the general protocols page