IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/08/05

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Now the Battle with I0500 Begins...

 * Kanamycin plates with pUC and I0500 TOP10 cells did not give any colonies
 * Incubation time with SOC for 2 hours is too short!
 * Plate out again with TOP10 incubated overnight at 37°C


 * TOP10 transformed with J63006 plated out on ampicillin plates (neat and 1/10 dil)
 * pUC control with 1/10 dil also plated out on ampicillin plates
 * Incubated at 37°C overnight

Anaerobic Condition and Iron Assay

 * Require nitrogen gas and paraffin oil
 * Received paraffin oil from the Pathology Dept, return excess after use
 * Nitrogen gas available in the Plant Dept
 * Dye for iron ions to be ordered from Sigma
 * Fur- mutant single colony plated on Kanamycin plate and inoculated into 75mL M9 medium prepared on 23/7/08
 * Mutants in M9 incubated at 37°C overnight