IGEM:MIT/2005/Friday 7/22 Meeting Agenda and Minutes

SubTeam Updates
(see individual wiki pages for details)
 * 1) Input Update (Maxine)
 * 2) Head Receiver Unit Update (Jenny)
 * 3) Receiver 1 Update (Will)
 * 4) Receiver 2 Update (Annie)
 * 5) Signal Processor Update (Ray)
 * 6) Actuator Update (Jessica - out)

Discussion of Main Issues
(make list as we go along. add an issue if you want).
 * 1) effect on viability: osmolarity of solution (pressure exerted on cell membrane) -- use sorbitol?
 * 2) testing scFv: need a const. promoter with RBS -- biobrick -- do western!
 * 3) *jenny is point person, get materials to do western early next week
 * 4) scFv linker-fusion issue
 * 5) *why is the linker different than other scFvs?
 * 6) *can we introduce another linker, to allow fusion to preserve geometry
 * 7) *can we still introduce linker using primers? expensive
 * 8) Making knock outs: lambda red system. use a non kan, non amp resistance
 * 9) *Counter selectable markers:
 * 10) *#PurF (involved in uracil synthesis) -- grow in media containing 5'FOA, will be processed by PurF protein, keep cells from growing?
 * 11) *#PheS