User:DavidRamos/Lab/2006-6-22

Morning

 * Incubator works.
 * Marine strain WH8102 came in the mail with SH media.
 * Went to MIT to pick up PCC7942 and 6803 from Peter Weigele at MIT.
 * Told us WH8102 will be way too hard to grow. We probably will go with the freshwater strains.
 * MIT food trucks go!

Afternoon

 * Made 1L of the following liquid media:
 * 20 mL 50x BG-11 stock
 * 980 mL deionized H20
 * Made 6 125ml liquid cultures in 250ml flasks:
 * PCC7942 streak w/toothpick
 * PCC7942 single w/toothpick
 * PCC7942 single w/o toothpick
 * PCC6803 streak w/toothpick
 * PCC6803 single w/toothpick
 * Control w/ toothpick
 * 16 hours light each day
 * Put flask holders in incubator and set shaker.
 * Open lids (parafilm with holes) to allow air exchange.
 * 30&deg;C