IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/21/Shan Shen

00:40 Digest SupD and terminator plasmids. System of SupD: System of terminator 1-23L:

1:00 Start to digest.

10:00 Electrophoresis to test the digestion products. The order and the amount of the samples: marker 5μL, SupD digestion, 1-23L plasmids, 1-23L digestion. Results: The digestions are successful.

11:10 Recycle the fragments of SupD.

13:10 Ligation of the vectors of 1-23L and inserts of SupD. System of ligation: 10μL

13:10 Ligation starts.

14:21 Miniprep bi-stable 1,2,3,SupD1 and 1-9H. Concentrations:

17:20 Transform the ligation products of SupD+1-23L backbone.