IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Design

 Specifications Design Modelling Implementation Testing/Validation  
 * Two parts were created to fulfil the specifications for the predator sensing test construct


 * a monocystronic J37020




 * a polycystronic J37016




 * These two parts are very similar and have the same inputs and outputs, therefore they can be analysed in exactly the same way

The differences are in the rates of production of GFP to LuxR:
 * Part J37016 will produce a polycistronic strand of mRNA and the rate of GFP to LuxR translation will depend on which RBS is farthest upstream.
 * Part J37020 produces two strands of mRNA they are produced by the same promoter, LuxpR but the seuqance surrounding luxpR differs between the two sites so there may be differing rates of transcription between the promoters controlling GFP and luxR.

Design choices

 * We need to fulfill all the properties listed in the specifications.
 * Based on the Quorum-sensing/quenching due to biochemical properties needed and BioBricks availability.