IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/13

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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WetLab
Extraction of genomic DNA for blue promoter is done, but we don´t have amplified.

Goals for next week

 * Search different copy number plasmid, with compatibility criteria.
 * Make a ligation between GFP-Reporter with plasmid.
 * Make primers to get Lux AB genes from Vibrio Fischery.
 * Think about extraction of Lux CDE looking in registry parts how they made it.
 * Make ligation for promoter and double terminator.
 * Get Luciferase, get mutant and clone it.
 * Primers used for blue promoter: BBa_K238013

Modeling
We´ve got the primary function for activity of Luciferase and concentration of cytosolic Luciferin.

Goals for next week

 * Finish modeling of Luciferase activity.
 * Start modeling of blue receptor.

Required data
propose, an association between promoter and quantity of reporter. Search protocols with measure about light and concentration.
 * We need to determinate promoter strength for Luciferase with experimental data. To do that we

TRpR → aλ[P1]

Promoter of Blue receptor → bλ[P2]

Where λ is light. Ps, concentrations.
 * Search buffer for best activity of Luciferase.

General

 * Everything must be in OpenWetWare before 7th June 2010.
 * Project information for wiki. History, Evolution, Basic physics concepts and mechanisms.
 * We are waiting for response in orders of bio-parts and else.
 * We propose an activity for Human practice.