User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/13

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01/13/10

 * &#x2713; Sequencing order: KAH126-133/MV2 transfection plasmids (primers DD122B, DD123B)
 * &#x2713; Minipreps: KAH117/MV2 (4)
 * &#x2713; Cultures: KAH94, 95 re-transformed (2 each); KAH117/MV2 (6) KAH118/MV2 (6)
 * &#x2713; Assemblies: KAH137, 138

Minipreps > Check with E/P digests

--> None are correct. Re-do digestion of inserts (KAH117 & 118) and ligation (into MV2) if recent attempt fails.

Assemblies
 * 1) KAH137: (1) KAH135/(E/S)/2353 + (3) NLSstop2(KAH010)/(E/X)/3200
 * 2) KAH138: (2) KAH136/(E/S)/1941 + "

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations


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