Berglund:Cellophane Method

Materials
Staining solution 500ml =	45-50% MeOH 100ml=	10% acetic acid 50mg=	0.05% Coomassie Brilliant Blue R-250 400ml=	deionized water Destaining solution (quick destaining) 45% MeOH 10% acetic acid deionized water Destaining solution (overnight destaining) 7% acetic acid deionized water Pre-drying solution 10% EtOH 5% glycerol deionized water

Methods

 * 1) After electrophoresis, stain gel for 20 to 40 minutes. No need to fix gel. Re-use staining solution.
 * 2) Destain for a few hours (quick destaining solution) or overnight.
 * 3) Soak gel in pre-drying solution for 10 to 20 minutes.
 * 4) Air dry between two sheets of cellophane.