IGEM:Stanford/2009/Notebook/Homeostasis/2009/08/04

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Daily Work
Goals
 * Ligate, transform, colony PCR...

Project/Accomplishments
 * Wet-Lab
 * Looks like success for some first rounds of cloning!!!
 * Now we have to do colony PCR to check....
 * Ligation for 1b


 * Tonight
 * restreaking
 * Liquid culture for stuff we are running low on (SoxR, BLH, etc)
 * Ran gel from colony PCR
 * Re-run in the morning (still getting used to new equipment and it looked funny)


 * Dry-Lab
 * [what projects occurred today?]
 * [what were the results?]

Problems
 * [what problems occurred? frustrations?]

Alternative Solutions
 * [solutions to the problems/future ideas]

Events

 * Graduate Student Board Meeting at 9am
 * Feedback
 * Better Intro
 * Change the first slide, it is scary. We could put into in other parts of the powerpoint
 * Too much text on the second slide
 * Visual of differentiation of T-cells
 * The Movie
 * exaggerate T-cell populations
 * possibly show a graph
 * Take out what you are not using... Is it necessary?
 * More contrast between before and after the implantation of the device
 * Take out the green dots
 * Think more about the purpose of our projest
 * How is it different from pre-existing medicine
 * Explain the location and environment/ symptoms of IBD
 * Naming= "NO sensor" "Trp sensor" explain RA- be VERY clear and simple
 * How is the device used to treat the disease? Why E. coli? What are the results?
 * Why is this a good solution? - greater context of what you are doing and AWESOMENESS!
 * Sell it!
 * The device level diagram slide
 * Divide into two different slides
 * Reduce the amount of details
 * Things to think about
 * How many background do we need to give? Background vs. Specifics in Final Presentation
 * How many people does this disease effect?
 * Talk more about the consequences of implanting the device
 * Keep all information simple, imagine your audience as really really lazy people
 * Impress your audience vs. communication
 * vBOL--talk more about what it is and what it could be used for
 * Slideshow general
 * 3 concepts per slide
 * Show the results, not the process
 * Inroduce vBOL symbols from the start and then use them throughout the presentation
 * Problems that we have been having
 * A biobrick standard that doesn't use Pst1 because the presence of restriction sites in gene sequence (Trp operon) that we can't mutate
 * Assign grad students for assay design
 * Trp operon--Meeting with Yanofsky

Comments
http://news.yahoo.com/s/ap/20090804/ap_on_bi_ge/us_arthritis_drugs_fda_warning
 * [leave a comment!]

Weekly Goals

 * [enter the team's weekly goals]


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