User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/11

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Summary

 * Blocking membrane of 08October2010 → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
 * Preparation of 2 10% Tris-glycine acrylamide gels
 * Dilution of samples, corrected to lowest concentration
 * Run at 200 V
 * 10% gel was cut in 4 for
 * GAPDH (1:2000, mouse (<40kDa))
 * RIIα (1:1000, rabbit (40 - 75kDa))
 * AKAP79 (1:1000, rabbit? (75 - 130kDa)) (Santa Cruz)
 * AKAP250 (1:1000, Goat, (>130kDA)) (Santa Cruz)


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