User:Torsten Waldminghaus/Notebook/Multiple Mutation Reaction/2008/04/29

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Multiple Mutation Reaktion
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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PvuII digest

 * Since the PCR was somehow not optimal (see yesterday) I just want to see if the PCR fragment can be cut with PvuII which should only cut when a right point mutation was inserted (see project dexcription).


 * digest:
 * 5 μL PCR product
 * 1.5 μL NEBbuffer 1
 * 1 μL PvuII
 * 7.5 μL A. dest

=> 1h 37 °C => add 5 μL sample buffer and freece at -20 °C