User:Robwarden/Notebook/Divalent Ligand Validation/2009/02/27

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 * style="background-color: #EEE"|[[Image:TBL.PNG|300px]] Divalent Ligand Validation
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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EcoRI Digest
10 μL DNA 2 μL 10X Buffer EcoRI ->  26 μL .5 μL EcoRI           ->  6.5 μL 7.5 μL MilliQ          -> 97.5 μL

Incubate at 37°C for 1hr.

Results

 * Clearly empty vectors for most clones.
 * α2,3,6 had a strong band at ~2.2kb (maybe the 1.5kb & .7kb bands?). Faint bands at 6.5kb and 1.8kb. Liquid cultures restarted by Lu.  Will be sent for sequencing on Monday 4/2/09.


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