User:Fermenter User/Notebook/BROM PICHIA SCREENING 2011 01 A

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM PICHIA SCREENING 2011-01-A

Day 1 (Jan. 10 Mon):
            12/20/2010 (15:00) Streaked yeast frozen stock onto 3 MD plates. Incubate at 30C for 5 days.

Day 5 (Jan. 14 Fri):
Select 10 colonies then incubate at 30C in MD medium.

Day 7 (Jan. 16 Sun):
Transfer colonies from MD medium to BMGY medium. Incubate at 30C.

Day 8 (Jan. 17):
Transfer colonies to BMMY medium.

Checked fourth colony. Contamination: No; OD: 12.1

Day 9 (Jan. 20 Thur):
0.5% MeOH induction

Day 10 (Jan 21 Fri):
0.5% MeOH induction

Day 11 (Jan 22 Sat):
Ran activity assays

1 OD:18.25 Slope: 0.83 Conc: 1.94 * 10^-4

2 OD: 16.75 Slope: 0.21 Conc: 4.91 *10^-5 Notes: Contamination

3 OD: 8.95 Slope: 0.95 Conc: 2.23 * 10^-4

4 OD: 16.4 Slope: 0.81 Conc: 1.895 * 10^-4

5 OD: 12.65 Slope: 1.05 Conc: 2.46 * 10^-4

6 OD: 15.55 Slope: 3.375 Conc: 7.90 * 10^-4

7 OD: 16.85 Slope: 0.97 Conc: 2.27 * 10^-4 Notes: Cells look dead

8 OD: 17.3 Slope: 1.49 Conc: 3.49 * 10^-4

9 OD: 16.1 Slope: -0.01 Conc:

10 OD: 16.55 Slope: 1.23 Conc: 2.88 * 10^-4


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