IGEM:IMPERIAL/2006/LabCalendar/2006-8-2

M9 Broth has been made up and is sitting in the fridge in T/L

Electroporations

 * We electroporated the following in the morning
 * I13521, 18I
 * 7A->3O DNA 2
 * DNA and competent cells with DNA are in cold room
 * Plates in 37C incubator in T/L

Maxiprep

 * Maxi-preped the following parts
 * 1I
 * 5M
 * 12D->24A colony 1, 3, 4
 * 16P
 * 2M
 * 7A->3O colony 2
 * Maxiprep DNA in fridge in T/L

Ligation

 * Redo ligation 12D->2H
 * Cut 12D with P & X, yellow buffer
 * Cut 2H with S & P, orange buffer
 * 30 uL digests
 * extract DNA successful, however, there was a faint larger band in the 2H lane, but we took out the larger band which corresponded most closely to 3381 bp, the expected size
 * Gene clean wash
 * Set ligations at 14:30, to be ready at 16:30
 * Gene clean ligation product
 * Electroporation completed
 * Incubated at 37C in Dr. Mann's Lab

Electrocompetent cells

 * Cells have been made competent...see protocol