User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/08

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

=Modeling: PoPS= From de article: "Measuring the activity of BioBrick promoters using an in vivo reference standard"
 * Promoter activity. Number of RNAp molecules that pass by the final base pair of the promoter and continue along DNA as an elongation complex.
 * PoPs (Absolute activity of promoter). Rate of successful mRNA initiation events per DNA copy of each promoter. Number of elongating polymerases per second (PoPs) exiting the promoter.
 * RPUs (Relative activity of promoter). Ratio of absolute activity of the promoter to the absolute activity of a reference standard promoter measure under the same conditions and with the same measurement instruments.

BioBrick Plasmids

 * [pSB3K3]
 * p15A origin of replication
 * 10-12 copy number
 * kn resistance


 * [pSB4T5]
 * pSC101 origin of replication
 * ~5 copy number
 * tet resistance


 * [pSB3C5]
 * p15A origin of replication
 * unknown copy number
 * cm resistance

Useful Links

 * [Promoter activity measurements]
 * [Technical Standards] (No text in the page)


 * }