IGEM:UC Berkeley/2006/Ligation

Basic Ligation
 * 1) Create Ligation Mixture:
 * 2) *5.5 uL water
 * 3) *1 uL ligation buffer
 * 4) *0.5 uL ligase
 * 5) *1 uL backbone (pAC997)
 * 6) *2 uL insert
 * 7) Let ligate at room temp for 30 minutes
 * 8) *Note: For the control, add 2 uL of water instead of the insert

Scaled-Up Ligations:
 * Use this for the construction of libraries.
 * 1) Create Ligation mixture:
 * 2) *69uL H2O
 * 3) *10uL Ligase buffer
 * 4) *10 uL backbone
 * 5) *10uL insert
 * 6) *1uL Ligase
 * 7) Incubate at room temperature for 60 minutes.