SSC

SSC buffer is named after its ingredients Saline (sodium chloride) and Sodium Citrate. It is used as a buffer for blotting, hybridisation, and stringency washes in Northern and Southern blots as well as DNA microarray.

1x SSC buffer

 * 150mM NaCl
 * 15mM sodium citrate

20x SSC

 * 800ml dH2O (RNase free if required)
 * 175.3g NaCl (3M)
 * 88.2g trisodium citrate (NaCit; 300mM; e.g. |SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC S4641)
 * adjust the pH to 7.0 with a few drops of 1M HCl
 * adjust the volume to 1L with dH2O
 * sterilize by autoclaving

using stock solutions

 * 3M NaCl
 * 300 mM sodium citrate, pH 7.0

protocols using SSC

 * Dahlquist:DNA Microarray Protocol
 * Endy:Northern Blot, 32P End-Labeled Probes
 * 20.109(S07): cDNA synthesis and microarray
 * 20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray