Springer Lab: 10x loading dye

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6x Orange G Loading Dye

1.	Use a 15 ml Falcon Tube.

2.	Fill it to 9 ml of 50% Glycerol.

3.	Add 6 ml Millipore water.

4.	Add a couple crystals of Orange G until the solution turns dark orange. This should only take 2-4 crystals.

Sources: http://www.bio-world.com/productinfo/1_10_121/2562/Orange-G-loading-dye-x-glycerol.html

http://www.thelabrat.com/protocols/DNALoadingBuffer.shtml