Template:SBB10 ConstructionFiles MaziarB sbb23

Construction of Zinc Finger Target basic part sbb23 Wobble mb0005/mb0006          (68bp, EcoRI/BamHI) Sub into pBca9145-Bca1144#5   (EcoRI/BamHI, 2057+910, L) Product is pBca9145-sbb23      {ZFNS target} mb0005  Forward construction of ZFNS target basic part gtcgagaattccgtagatctTGTTCGGAGCCGCTTTAACCCACTC mb0006  Reverse construction of ZFNS target basic part gcgatggatccAGCACTTCCACAGAGTGGGTTAAAGCGGCTCC 

JCA Notes
Construction of Zinc Finger Target basic part sbb23 Wobble mb0005/mb0006          (68bp, EcoRI/BamHI) Sub into pBca9145-Bca1144#5   (EcoRI/BamHI, 2057+910, L) Product is pBca9145-sbb23      {ZFNS target} mb0005  Forward construction of ZFNS target basic part gtcgagaattcATGagatctTGTTCGGAGCCGCTTTAACCCACTC mb0006  Reverse construction of ZFNS target basic part gcgatggatccAGCACTTCCACAGAGTGGGTTAAAGCGGCTCC
 * Prefix sequence should be ATG not CGT
 * Otherwise correct
 * Wiki link corrected