User:Wayne G. Shreffler/Notebook/Daily Log/2009/05/28

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*Wayne G. Shreffler 19:56, 28 May 2009 (EDT):

 * Went through R lesson with Shashi
 * Accepted ms from JACI to review
 * ~4 hours of patient work
 * reviewed Bert's fractions
 * [[Image:silverstain_052809B.jpg]]
 * Laverne will isolate more fraction 5 with 3 and 8 as controls
 * analyzed Bert's co-culture experiment:
 * 7-day co-culture of CFSE labeled autologous T cells with control, cpe, lps, or ct stimulated DCs together with increasing concentrations of retinol, retinal, or retinoic acid [[Media:RA_FP3_052809.jo.zip]]
 * no CCR9 induction
 * possible weak (very weak, ie wouldn't convince anyone) FoxP3 induction with exo RA in both CT and CPE
 * on the bright side -- clear induction of proliferation by CPE-stim DC over Medium


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