Klapperich Lab: Fluid Cell Tip

Overview

 * The following manual describes the special steps needed when using a fluid cell tip for submerged testing

Materials

 * 50 um Fluid Cell Tip
 * Tweezers
 * Superglue (if sample is not attached to AFM disk)
 * Millipore Water (if using Fluid Cell Tip)
 * PS-6 sheet from Vishay MicroMeasurements

Start Up

 * 1) Reserve the TriboIndenter at Yahoo Calendars
 * 2) *User Name: nanoindenter
 * 3) *Password: hysitron
 * 4) Turn on the computer if not already on

Changing the Tip

 * [[Image:diagram.jpg|Fig1. Diagram of TriboIndenter]]


 * 1) With the computer on and the TriboScan software closed, turn off the piezo and transducer controllers.
 * 2) Unplug the left cable from the transducer holder and the granite horizontal beam.
 * 3) Loosen the transducer holder by unscrewing the horizontal silver knob to the right of microscope.
 * 4) Slide the transducer holdlder upwards and out of the hood.
 * 5) Unscrew the tip using the tip tool and place in the hollow tube.
 * 6) Screw the tip in you desire stopping at the first sign of resistance.
 * 7) Slide the transducer holder back into place, connect the cables and retighten the screw.
 * 8) Turn both the peizo and transducer controllers back on.
 * 9) Rezero the tare value.
 * 10) Start TriboScan software.
 * 11) In the positioning window, go to Setup>>Tip Area Function. Select the correct tip area function.

Z-Axis Calibration in Air (Perform 1st and record in logbook)

 * 1) Change the microscope feedback gain to 100 on the TriboScope panel and in the software. From the Positioning window select Setup>>Piezo Controls and change the value for Front-panel Microscope Feedback Gain to 100. Minimize this window as a reminder to change the value back.
 * 2) From the positioning window, go to Setup>>Indent Open Loop Load Function.
 * 3) Set up the Load Function Editor to perform a 600uN 2-segment indent in air (AIR INDENT.ldf), for a total of 20 seconds. For soft materials, this load should be increased so that the air indent goes approximately 5um, but 600uN should be sufficient.
 * 4) From the Open Loop Load Function window, select Setup>>Advanced z-axis Calibration.
 * 5) Click "Calibrate Transducer." The instructions at this point from the pop-up windows are fairly self-explanatory.
 * 6) The calibration window will pop up. Check that the values in the table below are correct.
 * 7) Click done and follow the instructions.
 * 8) A graph will appear with a fitted functions of the Electrostatic Force constant as a function of displacement. Verify that that the fit is reasonable and close this graph.
 * 9) Make sure to write the ESF and ZVG values in the log book.
 * 10) Return the microsoft feedback gain to 1000 on the TriboScope and in the software by opening the window that was minimized earlier.
 * {| border="1"

! Parameter !! Correct Value
 * Machine Compliance || 4.0
 * Displacement Gain ||  100
 * Bias Offset || 0.2001
 * Disp Micro Feedback auto detect || Unchecked
 * }
 * Bias Offset || 0.2001
 * Disp Micro Feedback auto detect || Unchecked
 * }
 * }

Tip Optics Calibration

 * 1) From the positioning window, open the video window by clicking on the video button.
 * 2) Using the arrows in the Positioning window, move the optics to the PS-6 sample and find a "clean" spot to indent.
 * 3) You will probably need to disable the X-Y Safety Height. From the positioning window or the TriboScan Toolbar, select safety limits>>X-Y Safety Disable button.
 * 4) Change the video settings to white=30 and black=15 to increase contrast.
 * 5) Move up with the Z motor so that Z>15mm and tip will clear. Click on the create boundary button on the video window to create a sample boundary.
 * 6) From the positioning window, select Setup>>Indent Open Loop Load Function
 * 7) Open the load function, AIR INDENT.ldf.
 * 8) Zero the tare value.
 * 9) From the Positioning window, select Setup>>Calibrate Optics>>Stage Offset>>New Single Indent.
 * 10) The software will prompt you to adjust the tip height to about 1mm from the samples surface using the green arrows. Click OK after you have done this.
 * 11) After the indent move down in Z to be in focus on the sample. Do not move in X or Y.

Z-Axis Calibration in Water (Perform before indenting on sample)

 * 1) Change the microscope feedback gain to 100 on the TriboScope panel and in the software. From the Positioning window select Setup>>Piezo Controls and change the value for Front-panel Microscope Feedback Gain to 100. Minimize this window as a reminder to change the value back.
 * 2) From the positioning window, go to Setup>>Indent Open Loop Load Function.
 * 3) Set up the Load Function Editor to perform a 600uN 2-segment indent in air (AIR INDENT.ldf), for a total of 20 seconds. For soft materials, this load should be increased so that the air indent goes approximately 5um, but 600uN should be sufficient.
 * 4) Move indenter tip into the water more than 5 microns above the sample.
 * 5) From the Open Loop Load Function window, select Setup>>Advanced z-axis Calibration.
 * 6) Click "Calibrate Transducer." The instructions at this point from the pop-up windows are fairly self-explanatory.
 * 7) The calibration window will pop up. Check that the values in the table below are correct.
 * 8) Click done and follow the instructions.
 * 9) A graph will appear with a fitted functions of the Electrostatic Force constant as a function of displacement. Verify that that the fit is reasonable and close this graph.
 * 10) Make sure to write the ESF and ZVG values in the log book.
 * 11) Return the microsoft feedback gain to 1000 on the TriboScope and in the software by opening the window that was minimized earlier.
 * {| border="1"

! Parameter !! Correct Value
 * Machine Compliance || 4.0
 * Displacement Gain ||  100
 * Bias Offset || 0.2001
 * Disp Micro Feedback auto detect || Unchecked
 * }
 * Bias Offset || 0.2001
 * Disp Micro Feedback auto detect || Unchecked
 * }
 * }

Performing A Displacement Control Air Indent

 * 1) From the positioning window, go to Setup>>Indent Displacement Control Load Function.
 * 2) Open load function, "4000nm_AirIndent".
 * 3) Move tip to be in contact with the sample by watching the tare value

Contact

 * Jessica Kaufman
 * Eben Tessari