Wittrup: Dishwashing

Glass bottles, glass flasks
 * wash in dishwasher on Glassware cycle
 * autoclave (30 min sterilization, 30 min dry)
 * place on shelves in tissue culture support room (76-286)

Centrifuge bottles, Tunair flasks
 * wash in dishwasher on Plastics cycle
 * place on shelves in tissue culture support room (76-286)
 * NOTE:
 * plastic bottles and flasks are to be autoclaved as needed by the user
 * do not wash Tunair cap filters

Spatulas, stir bars
 * wash in dishwasher on Glassware cycle
 * place in containers on table in incubator / shaker room (76-284)

Pipet tips, microcentrifuge tubes, culture tubes
 * autoclave (30 min sterilization, 30 min dry)
 * place some on table in main lab (76-291)
 * place rest on shelves in dishwasher / autoclave room (76-292)

Pasteur pipettes
 * autoclave (30 min sterilization, 30 min dry)
 * place on shelves in tissue culture room (76-285)

Biohazard Waste
 * autoclave (90 min sterilization)
 * allowed to cool, then place each bag into a black plastic bag, secure, and set in hallway
 * NOTE:
 * just before autoclaving, open up waste bags to allow in steam during cycle
 * record tag numbers in biological waste log

Biohazard Sharps
 * transport all full sharps bins to the accumulation room
 * bring empty bins from the accumulation area to the tissue culture support room
 * NOTE:
 * empty bins are stored in the tissue culture support room (76-286)
 * full bins are stored in the incubator / shaker room (76-284)
 * the general accumulation area is room 76-268 (key on lab whiteboard)

Gel Buffers
 * TAE, MES, MOPS, and transfer buffers are in gel / FPLC room (76-287)
 * ensure all buffers are the above refill line throughout the week

By the end of the day on Friday...
 * unwashed glassware < 1 load
 * biohazard waste < 2 bags