SBB09 Construct1

PCR ca998/ca1034 on pSB1A2-Bca9033    (~350 bp, EcoRI/BamHI) Sub into pBca9145-Bca1089             (EcoRI/BamHI, Large) Product is pBca1020-Bca1034   {fdhF2}

ca1034 Reverse biobrick oligo for fdhF CACTTggatccAATAGGGGCAAACCGTGACGAC

PCR ca9394/ca9395 on colE2     (1758 bp, BglII/XhoI) Sub into pBca9145-Bca1089          (BglII/XhoI, 2057+696, L) Product is pBca9145-Bca9394      {ceaB}

ca9394  Cloning of ceaB   ccaaaAGATCTatgagcggtggcgatggacg ca9395  Cloning of ceaB   gctagCTCGAGttaGGATCCtcaatatgtcgcttaggtgt