IGEM:IMPERIAL/2009/M0/Assays

=Assays=

These assay are ordered in step-wise order based on what testings should be done first

Aims

 * Choose the secondary carbon sources most suitable for CRP promoter activity


 * Constitutive promoter activity will be tested using methods originally described by Jason Kelly. This assay should be used to provide the data required for the calculation of a PoPs output from the CRP promoter (for a given set of conditions).

Assay 1.1

Updated version of Assay 1.1

Aims

 * Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell

Assay 1.3

Aims

 * Relate the time it takes to reach switch point for varying initial concentrations of glucose.

Assay 1.2