Klapperich Lab:Notebook/Lab Meeting Notes/2008/12/16

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

16 December 2008 Lab Meeting Agenda

 * Check with B&G about compressed air and vacuum?

† Announcements
 * No meeting on 12/23 or 12/30
 * Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. Stating 1/8/09. See Lab Calendar for dates.

† Flu R01 † SEPSIS Project †RNA project † COBRA - problems: dead but green in the end - called tech support. - tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio. - omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute - try running the experiment first, then running the live/dead dye through second.
 * First samples?
 * Plaque Assay.
 * Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09).
 * Sonali working on first draft of new version paper by 12/25. Journal?
 * Hussam working on Lambda phage data.
 * Report to Jeff from Cathie.
 * Do cDNA prep. Spec, gels, send to Jeff B. Shipping?
 * Mark- Control circuit - This will be done by January.
 * Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)

- new set made, in glove box: the 1st 3 on the DOE. SERS signal strong, SEM today - need to add humidity as it changes even in the glove box - difficulty in locating the bacteria - needs much higher particle/bacteria ratio - experiments outside the channel first?
 * Substrates (JZ)
 * DOE for the experiment.
 * Colloid experiment. inconclusive. Look at SEMs
 * Prospectus framework/discussion: target the end of this week 1/23/09.

† Valve Array/Valve building (FJ) † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR '''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD ''' † RCA † Senior project † F31: Cochlea † Silica Optimization (Lambda):
 * Turn over platform to Teddy
 * TEDDY needs to meet with Mark.
 * So so close!
 * Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week.
 * Made new design for trapper.
 * Mask writer was broken.
 * Mask writer was broken.
 * IRB still going/ revisions by tomorrow to Ian. Target date 1/1/09
 * STAR-CD Renewal - January ($2K)
 * Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?
 * Look into Melting temp analysis on the 7300 - Done. what is contaminated?
 * Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD
 * Try microscope for looking a fluor. Learn how to look at the data in Image J
 * 52, 60, 65 C repeats.
 * Cathie submitted One pager Whitepaper. Waiting for response.
 * Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine
 * Set up adviser meeting Jan
 * Post Gent vs. Pre gent - 4 proteins. 3/4 no change.
 * Update by 12/24.
 * Do three elutions.
 * Next E. coli.


 * }