User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/18

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 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Time is running out!
        1. Ladder.
 * Today, I extracted plasmid from the green click bettle luciferase culture and I left a restriction for 20μl to see if the plasmid contains the luciferase. I also made a restriction with SPEI and PST I to be able to ligate it with LRE restricted with XBAI and PST I. 
 * I also made some plates with Cm 30 and Am 30 to do the cotransformation. 
 * In the afternoon, I ran a gel to check if the restriction went ok, the gel is shown next:
 * The lanes were as follows:

2. Restricted GCB luciferase with ECO RI and PST I. 

3. Restricted GCB luciferase with SPEI and PST I.<br/ >

4. Restricted LRE with XBAI and PST I.<br/ > <br/ > <br/ > <br/ > <br/ > <br/ > <br/ > 1, 2. Ladder<br/ >
 * Unfortunately, I keep observing these awful dots in the CB luciferases, that is why I ran a gel of both, the plasmid purification and the respective restriction with ECO RI and PST I to try to infer what is happening, the gel is shown next:<br/ >
 * The lanes from right to left are as follows:<br/ >

3, 5. Plasmid restriction<br/ >

4, 6. Plasmid purification<br/ > <br/ > <br/ >


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