User:Karmella Haynes/Notebook/Polycomb project/2010/06/22

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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06/22/10

 * &#x2713; Cell culture: expand healthy cell lines to 10 cm dishes

Cell culture > Failed to grow: > Successful growth: --> Process for chromatin and dox -/+ Western
 * 129-4 (try 129-3, 129-8)
 * 130-2 (last vial; 130-7 only alternate line; consider re-transfection)
 * 131-1 (try 131-1, 131-6, 131-9 and/or re-transfection)
 * 133-1 (do not try again, not needed)
 * 127-4
 * 128-8.3
 * 130-7

> Note: for publication, focus on the following set of lines
 * 1) KAH126 (huPcCD-ATF)
 * 2) KAH154 (huPcCD)
 * 3) KAH128 (flyPcCD-ATF)
 * 4) KAH157 (flyPcCD)
 * 5) KAH129 (fshPcCD-ATF)
 * 6) KAH156 (fshPcCD)
 * 7) KAH130 (hCBX8-ATF)
 * 8) KAH158 (hCBX8)
 * 9) KAH131 (flyPc-ATF)
 * 10) KAH159 (flyPc)
 * 11) KAH132 (no PcCD)


 * }