User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/23

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Bench work

 * 1) Analytical minigel
 * 2) 10μL 1KB ladder
 * 3) Hartings PCR sample
 * 4) 10μL gel-purified, double-digested pTXB1 from   earlier this week
 * 5) 5μL pTXB1(His) + BSA ligation from   yesterday
 * 6) 5μL pTXB1 + BSA ligation from   yesterday
 * 7) -12 --
 * 8) * the gel-purified digested vector floated away, so there is still a lot of ethanol in the solution.
 * 9) * EtBr stain
 * 1) -12 --
 * 2) * the gel-purified digested vector floated away, so there is still a lot of ethanol in the solution.
 * 3) * EtBr stain

Plates

 * No growth on either transformation plate.
 * Streaked E. coli Muratore:Materials/Strains/ER2566 stored @ 4°C

Analytical gel

 * Not surprising that nothing is visible:
 * The double-digested pTXB1 mostly floated away.
 * The ligation reaction lane has about 150ng total [DNA] (the comparable bands in the ladder have ~300ng). This might be visible, but, if the estimations of [DNA] are off, then it might not be visible.


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