User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/09

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WiFi coli: A communi-colight system
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

I gotta feeling!!!
      1, 5. Ladder.
 * After inactivating the restriction, I ran a gel to check if everything went on well. Though the gel merely shows a band, I consider ok to follow on with the ligation.
 * The lanes were as follows:

2. Click Beetle Green luciferase restricted with XBA I and PST I. 

3. Click Beetle Red luciferase restricted with XBA I and PST I.     <br/ > <br/ > -H2O ---> 7μL<br/ > -Buffer ligase ---> 4μL<br/ > -Vector (plasmid) -> 2μL<br/ > -Insert (CBG) ---> 6μL<br/ > -Ligase > 1μL<br/ > <br/ > <br/ > -H2O ---> 7μL<br/ > -Buffer ligase ---> 4μL<br/ > -Vector (plasmid) -> 2μL<br/ > -Insert (CBR) ---> 6μL<br/ > -Ligase > 1μL<br/ > <br/ > <br/ > <br/ > <br/ >
 * The next reactions describe the ligations to be done for a total of 20μl.<br/ >
 * 1: CBG (XBA I/PST I) with J23101 labeled as CBG + J23101 Mar and date. <br/ >
 * 2: CBR (XBA I/PST I) with J23101 labeled as CBR + J23101 Mar and date. <br/ >
 * I also replated 5 strains of pT7 + luciferase per transformation into kanamycin plates. <br/ >


 * }