User:Karmella Haynes/Notebook/miR Trigger/2011/01/17

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01/17/11

 * &#x2713; Planning: miR trigger construct tests

miR trigger constructs > Fun with control constructs and a truth table

--> Note: R and T are driven by constitutive promoters --> R = RFP fluorescence; T = CFP fluorescence

--> +/- m or r constructs will be built by including or excluding the miRNA target or Gal4 binding DNA elements --> Should be able to control luc miRNA with dox (JDS33-47 cells); if too leaky, use U2OS plain cells


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