Bioreactor Parameters

Medium parameters

 * Media formula
 * pH requirements
 * Medium change

Cell parameters

 * Generation time
 * Cell size
 * Cell density
 * Swimming
 * Senescence

Environmental conditions

 * Temperature
 * Light levels
 * Gas exchange (mech. also)
 * Shear stress (mech. also)

Kinetics

 * hydrogenases
 * nitrogenases

Reactor design

 * Vessel type
 * Valving, fittings, and tubing
 * Sealing/gaskets

Circulation during phases

 * Pumps
 * Gas diffuser
 * Gas exchange (bio. also)
 * Shear stress (bio. also)
 * Intermittent circulation

Transition from aerobic to anaerobic/sulfur stress conditions

 * Strainer, plunger

Hydrogen harvest

 * Temporary gas storage/bypass
 * Gas delivery to fuel cell (electrical also)

Electrical (fuel cell)

 * Power consumption
 * Theoretical power generation
 * Fuel cell type
 * Membrane type
 * Real time measurement of output/power
 * Gas delivery to fuel cell (mech also)
 * Gas purification
 * Power from fuel cell to pump
 * Charge battery

Mini-Experiments

 * Strain cells
 * Settling time
 * Gas chromatograph of headspace