IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation

 Specifications Design Modelling Implementation Testing/Validation  

Motivations

 * To see whether oscillations, which match are specifications, are produced. We will have fulfilled our specifications if the oscillations have the following properties:
 * Stable oscillations for greater than 10 cycles
 * High signal to noise ratio
 * Controllable amplitude and frequency
 * (Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification).

Protocol

 * A formal protocol has not been written.
 * Methods for testing under consideration are:
 * To aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay.
 * Use a biosensor to measure oscillating output directly (in this case we would be measuring change in pH in the extracellular medium).
 * Use High Performance Liquid Chromatography (more research is required).

Results

 * The prey construct has been built but still needs to be characterized
 * The predator construct still needs to be built

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