Klapperich Lab:Photopolymerization of PHEMA

Overview
This protocol outlines the photopolymerization of PHEMA.

Materials

 * 900 uL HEMA or modified monomer
 * 600 uL Ethylene glycol
 * 25 uL EGDMA (ethylene glycol dimethacrylate), crosslinker
 * 20 mg DMPAP, photoinitiator

Procedure

 * 1) Turn on digital dry bath. Add water to wells you will use. Set temperature to 85&deg;C.
 * 2) Add DMPAP to 2 mL centrifuge tube.
 * 3) Add HEMA to tube. Be careful to avoid air bubbles when pipetting.
 * 4) Add ethylene glycol to tube.
 * 5) Add EGMDA to tube.
 * 6) Heat mixture in digital bath until crystals are melted. Vortex.
 * 7) Add contents of tube to one well of a 6 well plate. Expose to UV light for 2.5 minutes.

Contact

 * Jessica Kaufman
 * Nathan Spencer
 * Eben Tessari