IGEM:IMPERIAL/2009/M2/Assays

=Assays=

Assay 2.1: Can the chassis produce colanic acid?
COMPLETE

While there are multiple strains of E.coli K12 that can produce colanic acid, it has been noted in the literature that some cannot. It is important that before selecting our K12 chassis, we ensure that it can produce colanic acid. A procedure for this has been prepared in Assay 2.1

Assay 2.1

Assay 2.2: Influence of carbon source on colanic acid production & population growth
COMPLETE

Once the glucose in the media has been consumed, the chassis will utilise a secondary carbon source to faciliate the production of colanic acid. Assay 2.2 serves to determine the secondary carbon source that is most suited to colanic acid production.

Assay 2.2

Assay 2.3: Sodium Acetate Encapsulation
INCOMPLETE

The encapsulation of colanic acid coated E.coli with sodium acetate has never been attempted. Theoretically, the highly negativly charged colanic acid coat should act as a nucleation site for Na+ ions in the sodium aceteate solution, but it would be wise to prove this in the lab as quickly as possible.

Since we can induce colanic acid production chemically, Assay 2.3 will provide a quick answer as to whether we need to investigate alternative secondary encapsulation technologies.

Assay 2.3

Assay 2.4: Sodium Acetate Exothermic Crystalisation
INCOMPLETE

Sodium acetate crystalisation is an exothermic reaction (hence the use of sodium acetate in hand warmers). If we want exothermic crystalisation to flip the kill switch, we need to model the heat that is released upon crystalisation.

Assay 2.4

Assay 2.5: Qualitative identification of construct-induced colanic acid production
INCOMPLETE

This assay indicates the successful expression of colanic acid inducing genes.

Assay 2.5

Assay 2.6: Quantitative analysis of construct-induced colanic acid production
INCOMPLETE

This assay faciliates an assessment of the protective effect of differing colanic acid profiles.

Assay 2.6

Assay 3.1: Production of Trehalose
INCOMPLETE

This assay confirms the production of trehalose.

Assay 3.1

Assay 3.2: Trehalose Functionality
NO LONGER REQUIRED - LITERATURE AVAILABLE IS SUFFICIENT TO ASSUME PROTECTION

This assay confirms the trehalose functionality.

Assay 3.2