Love:Protocols

Common Buffers and Blocking Solutions

 * PBS 10x stock solution

Buffers for Processing Protein Microarrays

 * Blocking solution stock for Antibody Arrays- PBS/3%BSA
 * Washing solution stock - PBS/1% Tween 20 (PBST 1%)

Tissue Culture Media

 * General DMEM media for hybridomas (DMEM-10)
 * Cell fusion-Myeloma partner cells
 * Cloning media for single cells (DMEM-20+HCF)

General Procedures

 * Freezing cells for storage in LN2

Hybridomas

 * Cell fusion-Myeloma partner cells
 * Cell Fusion-Fusion and Selection Medias

others

 * A20

Immunization

 * Mice immunization
 * test bleed + elisa

Probes

 * Peptide Probe Conjugation (streptavidin)
 * Peptide Probe Conjugation (KLH)

General Procedures

 * PDMS pouring
 * Extract unreacted monomer
 * Plasma cleaning
 * Preparing poly-lysine slides

Microengraving (&mu;En)

 * Genepix
 * μEn hybridoma screening
 * Counting

Autoclave

 * Dry Run
 * Wet Run
 * Biohazardous Waste