IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/23/Shan Shen

0:30 Digest SupD+1-23L 1, 2, 3 to test whether the clones are correct.

PCR to test at the same time.

1:20 Start to digest.

1:45 Start to PCR.

10:40 Electrophoresis to test the PCR products. The order and the amount of the samples: marker 5μL, colony 1 digestion products, colony 2 digestion products, colony 3 digestion products, positive control, PCR products of colony 1, PCR products of colony 2, PCR products of colony 3. Results: This result indicates that all the three colonies are correct.