User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/11

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Stop stimulation
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Add secondary ab (anti-mouse) for GAPDH repeat HT4 blot
 * Prepare 2 SDS gels for Co-IP
 * Stop stimulation

Method

 * SDS PAGE was prepared as described 01March2010
 * Co-IP samples of 05March2010 &  25Feb2010 were put to gel
 * 40 μL sample for 05March2010 samples
 * 30 μL sample for 25Feb2010 samples
 * 15 μL marker

Results

 * GAPDH--> failure, picture Anouk?
 * Viability staining

Conclusion

 * ✅ Ow..

Run 3: Ht31/S0

 * 03March2010 Counting cells D9 (P21)/D12 (P25)
 * 09March2010 Putting to S0 D9 (P21)
 * 10March2010 Stimulating cells D9 (P21)
 * 11March2010 Ending stimulation & Viability D9 (P21)


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