IGEM:Stanford/2009/Project Homeostasis/Primer/Primersdoc

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Primers Compilation


 * [[Media:Finalized_Primers.doc| Finalized Primer Sequences Document]]

Device 1:
SoxR (Escherichia coli E24377A)

Forward: TATAgaattcgcggccgcttctagATGGAAAAGAAATTACCCCGC Reverse: TATAtactagtagcggccgctgcagTTAGTTTTGTTCATCTTCCAGCAAGCGTG

SoxS promoter (Created by Joe 7-6-09) Forward: TATAgaattcgcggccgcttctagagAATCGCTTTACCTCAAGTTAACTTG

Reverse: TATAtactagtagcggccgctgcagCAGTTCGTTAATTCATCTGTTGGG

Blh (uncultured marine bacterium 66A03) Forward: TATAgaattcgcggccgcttctagATGGGTCTGATGCTGATTG

Reverse: TATAtactagtagcggccgctgcagTCAGTTTTTGATTTTGATACGGG

RalDH(II) (Rattus Norvegicus*) Forward: TATAgaattcgcggccgcttctagATGCCCGGCGAGGTGAAGGC

Reverse: TATAtactagtagcggccgctgcagTTAGGAGTTCTTCTGGGGGATC

Device 2:
New IL-6 fusion gene (Created by Joe 7-6-09)

Forward: TATAgaattcgcggccgcttctagATGCCAACAATAACCACTGC

Reverse: TATAtactagtagcggccgctgcagTTATGCTGATGCTGTCAAAG

HlyC (from E. coli str. CFT073)

Forward: TATAgaattcgcggccgcttctagagTTGGTTTGCTTTTTTTTACCTGCCACC

Reverse: TATAtactagtagcggccgctgcagCTCTTAACCTGTTAATG

HlyB (from E. coli str. CFT073) Forward: TATAgaattcgcggccgcttctagagGGAGTCATAATGGATTCTTGTC

Reverse: TATAtactagtagcggccgctgcagGTTAGTCTGACTGTAACTG

HlyD (from E. coli str. CFT073) Forward: TATAgaattcgcggccgcttctagATGAAAACATGGTTAATGGGGTTCAGCG

Reverse: TATAtactagtagcggccgctgcagCTCTGAGACTTAACGCTCATG

TolC (from E. coli str. CFT073) Forward: TATAgaattcgcggccgcttctagagGCAAATGAAGAAATTGCTCC

Reverse: TATAtactagtagcggccgctgcagCGTCATCAGTTACGGAAAGGG

TrpR (E. coli str. IAI1) Forward: TATAgaattcgcggccgcttctagATGGCCCAACAATCACCCTATTCAGC

Reverse: TATAtactagtagcggccgctgcagTCAATCGCTTTTCAGCAACACCTCTTCCAGCCACTGGC

Trp Promoter and Operator (E. coli: no specified strain ) Forward: TATAgaattcgcggccgcttctagagCTCAAGGCGCACTCCCGTTCTG

Reverse: TATAtactagtagcggccgctgcagTCGATACCCTTTTTACGTGAACTTGCGTACTAG

HlyR: Since HlyR is not a protein. This sequence is sequenced as a chunk with HlyA,HlyB, HlyC. Thus only one primer is needed. primer:

TATAtactagtagcggccgctgcagGAATTCCAAGCGAAGTCC