User:Tk/Notebook/MF/2009/03/24

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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 * style="background-color: #F2F2F2" align="center"|  |Main Page


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Recombination cassette construction

 * primers used for recombination experiments 4/15/03


 * These are primers for the flanking regions around the Sau3AI methylase and restriction enzyme genes


 * Mfl2I-up-F TCCAAATAGTGTGGATAC 352951 - 352968
 * Mfl2I-up-R  aag aca tct atg ttt ttt tac 353389 - 353409


 * Mfl2I-dn-F gag att att atg gca aaa g 356063 - 356081
 * Mfl2I-dn-R  aat atg agt aaa ttc ac 356763 - 356779


 * These are primers for the PheS gene
 * PheS-mf-F CCAAATAACTCTGTTATTGG  460253 - 460272
 * PheS-mf-R  at gtt cta cct act ctc c 459091 - 459108
 * PheS-mf-F2 ctc tgt tat tgg tat agt ta 460245 - 460264


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