User:Brian P. Josey/Notebook/2009/12/02

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Gel of Ligation
I set up a gel of the ligation from yesterday this morning, as soon as it develops and I get a good picture of it, I will post it here. Right now I am waiting for the gel to harden.



Unfortunately the ligation did not work as we had hoped. We ran a nanodrop on it to determine the concentration, which worked out to 3.9ng/ul.

To Do While Ant's in New York

 * Practice the tethering reaction and try to get some to work.
 * Try the different length tethers to get used to looking at them.
 * Run a PCR of the double stranded DNA.
 * Find the missing primer
 * Remember the concentrations are different for the dNTPs
 * Try the concentration of Mg2+ at what is given, and half that.
 * Read through the protocols and write up thoughts and notes
 * Math quiz, volume integrals and divergence theorem


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