IGEM:MIT/2007/Notebook/2007-6-29

Agenda for Friday, June 29

 * Morning
 * pBAD pickup
 * Is OmpA crucial to bacterial cell survival?
 * Finalize oligos (restriction sites)
 * Research "red team" stuff -- ion exchange
 * Brian -- stainless steel, nylon binding?
 * Check on FhuA and pSB3K3 colonies


 * Afternoon
 * Look for metal inducible promoters (Mer) to fuse to GFP/something FP/whatever
 * Order above oligo
 * Find/finalize vector (pBAD33?)
 * Have everything ordered by the end of today!!


 * Question to think about: metal ion diffusion into the cell or binding at the surface?

pSB3K3

 * 9AM - Good news, all of the colonies we picked last night are cloudy today! :D