IGEM:Yale/2010/Notebook/2010/07/15

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

SpeI Activity Assay

 * To determine if SpeI being used was functional, ran a gel of circular B0015 vs. B0015 digested with SpeI. Gel was 1.0% agarose and run at 90 V with a 1 kb ladder. The result confirmed the activity of SpeI. [[Image:150710_speItest.jpg|thumb|Gel to determine SpeI activity From left, Lane 1: 1 kb ladder, Lane 3: undigested B0015 (dimer and monomer visible), Lane 5: B0015 from SpeI digest, at 3.2 kb as a linear fragment ought]]


 * }