Endy:Screening plasmid/v1.0/Hairpin

Constructs
We are building constructs with and without the hairpin and with and without the terminator. These are downstream of two different strength constitutive promoters ( R0040 and R0050 ).


 * I13521 - R0040.B0034.E1010.B0015
 * I13572 - R0040.hairpin.B0034.E1010
 * I13573 - R0040.hairpin.B0034.E1010.B0015
 * I13574 - R0050.B0034.E1010.B0015
 * I13575 - R0050.hairpin.B0034.E1010.B0015
 * I13576 - R0050.hairpin.B0034.E1010

Related parts
These haven't been cloned into BB, but were just PCR'd out
 * I13570 - hairpin.B0034.E1010
 * I13571 - hairpin.B0034.E1010.B0015
 * I13577 - hairpin (this hasn't been PCR'd)

Sequence Verified

 * I13521 - R0040.B0034.E1010.B0015
 * I13573 - R0040.hairpin.B0034.E1010.B0015
 * I13575 - R0050.hairpin.B0034.E1010.B0015
 * I13574 - R0050.B0034.E1010.B0015
 * We also have a version of I13572 with AC inserted in position 3 of the hairpin (otherwise perfect).

Failed

 * I13572 - R0040.hairpin.B0034.E1010
 * AC insert in position 3 of the hairpin
 * I13576 - R0050.hairpin.B0034.E1010
 * Failed at ligation/transformation step (no colonies).