User:Fermenter User/Notebook/BROM C4

{| width="800"
 * style="background-color: #cdde95;" align="center"|
 * style="background-color: #cdde95;" align="center"|




 * align="center" style="background-color: #e5edc8;" |


 * colspan="2" style="background-color: #F2F2F2;" align="right"|Customize your entry pages 
 * colspan="2"|
 * colspan="2"|
 * colspan="2"|

Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM C4 (F7)

Day 0 (Jun 1 Mon):
Change #1: Media composition: JR                 6/1/2009 (10:30) Bring media to fermenter room            6/1/2009 (11:00) Assemble bioreactor 6/1/2009 (11:00) Add 10 ml of Antifoam 6/1/2009 (11:10) Start Sterilization (Liq 45 min) 6/1/2009 (12:50) Cooling of bioreactor (Temp setup) 1 L/min air, 400 rpm 6/1/2009 (12:55) Start Fermentation Software for Fermenter #7 6/1/2009 (13:05) Connect Acid (250 ml) and Base (340 ml after prime pump and bring pH to 7.0) Start pH control

Day 1 (Jun 2 Tue):
6/2/2009 (09:00) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 6/2/2009 (09:00) Set dO2 6/2/2009 (09:00) Completion of bioreactor set up: Ready for inoculation

            6/2/2009 (11:15) Inoculation: inoculation volume (300 ml) Fermentation 0:00             6/2/2009 (11:20) Sampling  Fermentation 0:05 Cell weight (10 mg), Activity (65%, x100), Activity (25%, x1000)             6/2/2009 (17:55) Sampling  Fermentation 6:35 Cell weight (77 mg), Activity (92%, x100), Activity (65%, x1000)

Day 2 (Jun 3 Wed):
           6/3/2009 (8:15) Fermentation 09:00 Replace O2 tanks             6/3/2009 (9:30) Sampling  Fermentation 22:05 Cell weight ( 156 mg), Activity ( 90%, x100), Activity (   77 %, x1000)

    6/3/2009 (10:45) Fermentation 11:30 Harvest Fermentation


 * }