User:Mark X. Ling/Notebook/Micb 323/2009/01/27

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Part 1: Heat-shock ligation from previous ligation product

 * time of mix(on ice):1:13 - 1:45
 * Time put in shaking water bath:1:55 - 2:55

Cacl2/Heat shock ligation (student A)

 * By Crystal Lee

Electroporation (Student B)

 * Time on incubation:2:30 - 3:30
 * original on incubation: 2:40 - 3:48
 * Notes:
 * the Electroporated samples were not shaking for unknown amount of time.
 * Original were incubated for 1 hr and 8 minutes.


 * plate 0.1mL therefore undiluted = 10^-1

Post Lab Comments

 * transformation by electroporation is better: was faster to use
 * after seeing the colonies, it seems to be better method also.
 * Plating went smoothly
 * there were inconsistency in # of colonies.
 * may not have let the plate dry for long enough: there were lines of colonies in numerous plate.


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