IGEM:metu/2009/Notebook/wound dressing/2009/09/07

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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07.09.2009
1. Isolations of 4.07,2.09,1.09,2.07,5,1.07,8.07,6,10M Lysis,7,9,BB were made.

2. Digestions were made. The following amounts are added to microtubes;

1.07,1.09,           Buffer Tango         2 µl Spe1                2 µl DNA                16 µl  =20 µl 2.07,2.09,           Buffer O             2 µl Pst1                2 µl DNA                16 µl  =20 µl 1.09 control,         Buffer Tango         1 µl Spe1                0 µl DNA                 9 µl  =10 µl BB,2.09 control,      Buffer O             1 µl Pst1                0 µl DNA                 9 µl  =10 µl