User:Karmella Haynes/Notebook/Polycomb project/2010/11/30

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

11/30/10

 * &#x2713; ChIP: sonicate U2OS FTRx chromatin; H3K27me3 IP overnight

Sonication > U2OS FTRx cells
 * 1) Double cross-linked "dx" (DMA, CH2O)
 * 2) Single cross-linked "fx" (CH2O)

IP: antibody binding Note: Out of rabbit IgG! Use rabbit α-myc
 * 1) FTRx dx, H3K27me3 07-449
 * 2) FTRx dx, rabbit IgG
 * 3) FTRx fx, H3K27me3 07-449
 * 4) FTRx fx, rabbit IgG

--> Rotate at 4°C/ overnight


 * }