User:Tk/Notebook/MF/2009/03/23

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
 * style="background-color: #F2F2F2" align="center"|  |Main Page
 * style="background-color: #F2F2F2" align="center"|  |Main Page


 * colspan="2"|
 * colspan="2"|

Genomic DNA Prep

 * all cultures grew
 * plate out cultures on 1161 plates w/o antibiotic
 * prep genomic DNA from 12 ml cultures
 * save 20% glycerol stocks of all cultures


 * potential problem: overgrowth of culture may have killed some of the cultures


 * Use OWW CTAB protocol for genomic DNA scaled up 6x


 * 3.6 ml TE
 * 18 ul of proteinase K
 * 90 ul of 20% SDS (instead of 10%)


 * problem resuspending the pellets




 * }