Chang Lab:Notebook/CBE/08/146/2008/12/10

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title

 * Repeat innoculum preparation as on 10th Dec.
 * This time only using 1 loop instead of the the 5loops for 150ml broth. Reason is to reduce the number of colonies on the agar petri dish so that counting would be more manageable.


 * Absorbance reading started at 530pm.
 * Number of time points reduced to 0th hr, 3-5th hr, 14th, 16th and 24hr.
 * Serial dilution was increased 10^-6, 10^-8 and 10^-10 for (1st 8-hr incubation; while plate 10^-8, 10^-10, and 10^-12 for subsequent hours.


 * Note
 * Growth curve of 2nd mid log phase is similar to that of the 1st. Mid log phase of E coli is 5-6th hr.


 * }