IGEM:Peking/2007/Switch-Notebook/2007-7-24

Experimenter: LD & CXY

Overnight ligation of pLX007/8/9^^ and lacZa^^

 * Plasmid: 4.5uL, lacZa: 0.5uL

Retract from electrophoresis jel: lacZa-T^(XhoI/SalI)


Not retracted because the concentration is too low.

Temperature PCR of RS1-3, RD1-3 from Prec-T

 * Template: 1uL
 * Buffer: 5uL
 * dNTP: 5uL
 * Primer_F Rec_S_1_F or Rec_D_1_F: 1uL
 * Primer_R Rec_S_1_Rnew: 1uL
 * Ex-taq: 0.25uL
 * H2O: 37uL
 * Total: 50.25uL


 * Product: RS1, RD1
 * Temperature gradient: 53.2C, 58.1C, 63.5C, 68.1C



l to r: RD1(1-4), RS1(1-4), 100kb ladder

retract: RS1(53.2C), RD1(53.2C)


 * Template: 1uL
 * Buffer: 5uL
 * dNTP: 5uL
 * Primer_F Rec_S_2/3_F or Rec_D_2/3_F: 1uL
 * Primer_R Rec_S_2/3_Rnew: 1uL
 * Ex-taq: 0.25uL
 * H2O: 37uL
 * Total: 50.25uL


 * Product: RS2/3, RD2/3
 * Temperature gradient: 68C



l to r: RS2, RS3, RD2, RD3, 100kb ladder

retract: RS2, RD2