User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/02

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07/02/11

 * Cultures/ minipreps: KAH200 (4)

Minipreps > Check with E/P digests

Assemblies
 * 1) BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size
 * 2) BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size

> Digests (Fermentas FD) --> Specific notes

> Measure conc.'s

> Dephosphorylation (Roche)

> Ligations


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