User:Anthony Salvagno/Notebook/Research/2011/05/04/Breaking Down Tethering

I want to learn more about the tethering process and what each component does. This way if something goes wrong it will be easier to troubleshoot then if I didn't know. I'm sure this will be an ongoing project.

Components and Properties

 * anti-dig - needed for tethering
 * PBS
 * DNA - required for tethering
 * microspheres - required for tethering
 * BGB - thought to block surface and keep microspheres separated
 * popping buffer - for use with enzymes primarily, but used for generic tethering
 * EDTA - to sequester Mg++ ions
 * NaCl
 * Sodium Phosphate
 * Tween