IGEM:PennState/Labbook/Nimrah Ahmed/2008/05/15

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Circuit project ideas:

 * Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf

Sequential cell color change

 * ex. Green->Red->Yellow->repeat.
 * System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
 * Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter

Components

 * Organic compound: hydrophobic, heavy (won't evaporate), is recognized by cell
 * Recognition compound(s): recognize organic compound and synthesize/activate something that activates promoter
 * Promoter: probably lac
 * Color proteins

Circuit
O -> A

A+B -> C, GFP

A+C -> B, YFP


 * Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
 * Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.

Options:

 * Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
 * Rather than shake, stir by thermal convection or a magnetic stirrer

(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)

Nimrah Ahmed 12:26, 15 May 2008 (EDT)


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