IGEM:Harvard/2006/vlau/Notebook/2006-7-12

Protein & DNA-staining Experiment
1. Rxn Mixtures

2. Protocol - gel run @ 25V, 4C for 2.5 hrs. (dye appears to have diffused sideways rather than traveling downwards) - gel cut into DNA & protein sections after removal from cartridge (slight tearing towards bottom) - protein section stained w/ GelCode Blue Stain (Coomassie Blue) for 1 hr. - DNA section stained w/ 10 EtBr in 100 mL Tris-glycine buffer for 1 hr.

3. Results - both sections showed no bands - possible reasons: buffer? the gel itself?