SBB10 projects

Assay Teams
Team 1: Toxicity and Expression Team 2: Lysis and Transposition Team 3: Origin stability and copy number Team 4: Toxicity and Expression Team 5: ZFN and phiC31 Activity

Composite Parts
[[Media:140L_parts1_032910.xls | Composite Parts List]]

Part Project Pages:
11097-Zinc Finger Domain (zf+), Zinc Finger Domain (zf-) 11956-piggieBac 5'TR, N15 Protelomerase 13567-piggieBac 3'TR, N15 Protelomerase 14224-tos site of N15, piggyBac transposase 15924-sleeping beauty 5'TR, PhiC31 Integrase 15954-sleeping beauty 3'TR, PhiC31 Integrase 18094-FokI Cleavage Domain (fok-), P9 rep Protein 19244-FokI Cleavage Domain (fok-), P9 origin of replication 19329-Tn5 3'TR, Gentamicin resistance gene 19395-Ala6 linker, Tn5 Transposase 19607-FokI Cleavage Domain (fok+), Tn5 5'TR 20154-FokI Cleavage Domain (fok+), Zinc Finger target 26253-CA42 origin of replication, phiC31 attB 27095-CA42 rep Protein, phiC31 attP 28190-O99 origin of replication, O99 regulatory RNA, Constitutive RFP 29071-O99 rep Protein, CA42 rep Gene, Pre-Pro sequence 37613-P9 regulatory RNA, CA42 regulatory RNA, Pre-Pro sequence 37738-O99 rep Gene, Pbad promoter, Constitutive promoter 38352-Sleeping beauty (SB100x), Magnesium-repressed Promoter, Nuclear localization peptide

Families by part type:
Complete Gene part family Promoter and CDS, no terminator part family Promoter and RNA, no terminator part family Enzyme family DNA binding domain family cis element family Rep protein family Antibiotic marker family

Families by device grouping:
N15 Protelomerase family piggyBac family Tn5 family Sleeping Beauty family ISY100 family phiC31 integration family Zinc Finger Nuclease family Zinc Finger Transposase family Orthogonal Replicon family

Experimental notes:
Part requires RBS calculator Part requires an EcoRI/BamHI Transfer Part requires Gene Synthesis