S. cerevisiae genotypes

Common yeast strains
van Dijken00 describes the characteristics of many common laboratory strains.

S288C
MAT&alpha; SUC2 gal2 mal mel flo1 flo8-1 hap1
 * gal2-; does not use galactose anaerobically
 * does not form pseudohyphae
 * ATCC: 204508
 * Mortimer86

AB1380
MATa ura3-52 trp1-289 lys2-1 ade2-1 can1-100 his5 &rho;+ &psi;+
 * ATCC 204682, 20843, 201447
 * AB1380 is MATa not MAT&alpha; in contradiction to the article in Science 236: 806-812, 1987
 * Burke87

J57D
MATa ura3-52 trp1 ade2-101 can1-100 leu2-3,112 his3-2,15
 * used in Haldi96 for the mouse genome project
 * from Vladimir Larionov
 * Zhong98 claims MAT&alpha; and his3-6
 * several times better transformation efficiency than AB1380

W303-1a
MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ybp1-1
 * Open Biosystems: YSC1058
 * Rothstein notes
 * Veal03

VL6-48N
MAT&alpha; trp1-&Delta;1 ura3-&Delta;1 ade2-101 his3-&Delta;200 lys2 met14 cir&deg;
 * high transformation efficiency strain for spheroplast YAC construction
 * Vladimir Larionov papers on TAR (transformation associated recombination)
 * generated from VL6-48 by substitution of the KanMX cassette into ura3-52
 * Construction: Kouprina98 (from VL6-48); Larionov96 (from YPH857, Sikorski89, Larionov94)

YPH499
MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-&Delta;63 his1-&Delta;200 leu2-&Delta;1
 * derived from S288C
 * contains deletions of most selectable markers
 * retains the promoter for the trp gene, thus homology with that plasmid sequence
 * gal2-; does not use galactose anaerobically
 * ATCC:204679
 * Sikorski89, Sobel99

YPH500
MAT&alpha; ura3-52 lys2-801_amber ade2-101_ochre trp1-&Delta;63 his1-&Delta;200 leu2-&Delta;1
 * derived from S288C
 * contains deletions of most selectable markers
 * retains the promoter for the trp gene, thus homology with that plasmid sequence
 * gal2-; does not use galactose anaerobically
 * ATCC:204680
 * Sikorski89, Sobel99

Window Strains

 * Strains are derivative of YPH925


 * Strains have gaps in the chromosome size range to allow gel extraction of YACs with specific sizes. See Hamer95.


 * PFG conditions are BioRad Chef 1% SeaPlaque GTG agarose at 6 V/cm 0.5x TBE 12 C


 * YACs can be transfered to these strains by kar1- mating, see Hugerat94 and Spencer94.

YBLW1A
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15 cys3
 * Window 150-280 Kbp
 * PFG 15-25s, 24 hrs
 * Genotype is listed for YBLW1; YBLW1A may have differences, but is the strain we have.

YBLW2
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15 met10 thr4
 * Window 250-450 Kbp
 * PFG 20-35s, 24 hrs

YBLW3
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15 met10 thr4
 * Window 310-590 Kbp
 * PFG 35-60s, 24 hrs

YBLW4
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15 thr1
 * Window 450-680 Kbp
 * PFG 50-70s, 24 hrs

YBLW5
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15 met14
 * Window 590-755 Kbp
 * PFG 50-80s, 36 hrs

YBLW6
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15
 * Window 680-950 Kbp
 * PFG 60-100s, 36 hrs

YBLW7
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15
 * Window 810-1120 Kbp
 * PFG 70-120s, 48 hrs

YBLW8
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15
 * Window 985-1640 Kbp
 * PFG 80-130s, 48 hrs

YBLW9
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2R kar1-&Delta;15
 * Window 1140-2000 Kbp (upper range varies due to the presence of ribosomal repeats in chromosome XII)
 * PFG 100-160s, 48 hrs