IGEM:IMPERIAL/2007/Calendar/2007-9-11

We are in Week 10  of the iGEM project

Project Calendar
name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7

Vesicles

 * Put pTet-GFP into vesicles - need to see what the result is tonight
 * Looking at equations relating expression in vesicles - to help with data analysis

Dry lab

 * Doing the wiki
 * graphic design

Experimental design (wet lab)

 * 24 hr experiment on CBD construct - put on hold
 * going to sort the methododlogy first
 * Design experiments to test affect of evaporation and how to reduce it
 * Mineral oil - possibility but affects oxygen supply too
 * Today testing:
 * if evaporation prevented by layer of oil
 * if layer of oil affects the reading of tehe fluorometer
 * Test will be done tomorrow at 37&deg;C using either mineral or paraffin oil (depending on results from today's experiment)
 * Will test for affect on expression of the samples due to oil (blockage of oxygen)
 * T7 should be ready by tomorrow

Problems

 * Twinkle can't be moved from the teaching lab - need to talk to biochem lab to use theirs
 * DsRED express - will be delivered earlier next week (problem with quality control)
 * GFP mut-b3 from the lab - needs to be PCRed and cloned with a tag
 * LuxR
 * purity < 50%
 * Can't get exact [LuxR]
 * Need LuxR 10 x the amount of DNA for ID construct

CBD
Meeting scheduled with Vincent after tomorrow's debrief
 * Refocus CBD application/specification
 * Results so far don't comply with the specification
 * Alex - find out what is stopping expression of the pTet construct below 10 and over 45&deg;C
 * Might be useful before meeting to get min working temperature
 * not possible as experiments put on hold until methodology improved