Sauer:HPLC protocol

Buffers:

 * A: 0.06% TFA in dH2O.
 * B: 0.054% TFA in 80% Acetonitrile
 * When preparing, filter with Chemically Resistant ZapCap.


 * 1) Turn on pumps from bottom to top. (there are 4 parts that need to be turned on)
 * 2) Uncap waste container
 * 3) Turn on computer, open Class VP, open Instrument 1.
 * 4) To turn on computer control, click on icon that looks like a stamped letter. (upper left-hand corner.)

Column should be stored in 100% Buffer B.

Flush into Buffer A before run.
 * Under Instrument Set-up, set %B to 0, and T.Flow to 1mL/min.

To run, click Download

Select a name for your file. Make sure this will be in your own directory.
 * 1) To run a method, open method (start with basic.met, obtain copy and create your own folder for this.)
 * 2) To start the method, click on the Blue arrow.

Loading Sample:

Triggering the Run: 
 * 1) Wash the syringe a couple times with Buffer A, shooting the waste into the waste container.
 * 2) With the knob turned all the way to the right (clockwise) insert the needle.
 * 3) Turn the knob counterclockwise all the way, and then inject your sample.
 * 4) Turn the knob back in the clockwise direction.


 * 1) Press Start on the control box.
 * 2) To trigger the run to start, form a circuit between the two exposed wires.

Shutdown:

(otherwise the next person could end up with your sample as a contaminant)
 * 1) Go to 100% Buffer B for 10 min.
 * 2) Change flowrate to 0.
 * 3) Close down Class VP
 * 4) Shut-down pumps from top to bottom.
 * 5) Clean the Syringe with Buffer A. Leave syringe in HPLC.