User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/20

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05/20/11

 * &#x2713; Assembly: Remove PEST from CFP reporter gene; KAH181
 * &#x2713; Retransform: KAH57

Assemblies
 * 1) KAH181: Kozak/(S/P)/~3200 &#x2713;  + (1) KAH57/(X/P)/1426

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

--> Add 10 μL lgn rxn. to 30 μL Dh5α Turbo --> Also do retransformation of KAH57


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