IGEM:Peking/2007/Switch-Notebook/2007-8-3

Experimenter: LXL

Colony PCR test of CD2, RD2, RS3-pMD18-T
Primers:  MBF/R

6 of each type are correct and sent for sequencing.

Digestion of fragment and plasmid
2uL   pLX007(4) 2uL   XhoI 2uL   SalI 10uL   10*H buffer 84uL   H2O

100uL   total

2uL   SD-lacZa-pLX003 2uL   XhoI 2uL   AatII 10uL   T buffer 10uL   BSA 84uL   H2O

100uL   total

22uL   T1T2^^(AatII/XhoI) 2uL   XhoI 2uL   AatII 10uL   T buffer 10uL   BSA 54uL   H2O

100uL   total