User:Jessie Wang Lectures Mod1

Lecture 0: Intro to 20.109 (9/6)

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Lecture 1: Intro to Genome Engineering (9/11)

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Lecture 2: Genes as Parts (9/13)

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Lecture 3: Synthetic Biology (9/18)

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Genetic Engineering vs. Synthetic Biology
Genetic Engineering Tools: 1. rDNA (1970s) - cut and paste pre-existing DNA fragments 2. PCR (1985) - amplify and modify exiting DNA 3. Sequencing (1977) - read DNA into A,T,C,G

Synthetic Biology: 4. Automated Construction (2004) - DNA synthesis (1987) 5. Standards (2001) 6. Abstraction (2003)

Functional Composition
Switch - bistable

GDP - | G-Protein | <--> GTP - | G-Protein | Riboswitches
 * Ribozyme
 * Aptamer
 * Computation Domain

Construction

 * Phosphoramidites - (A,T,C,G)
 * Came from salmon milt.
 * 10 g x 1 mol/1000 g x 6E23 molecules/mol = 6 x 10^21 molecules of a particular base
 * 10^10 people/Earth x 10^10 bp/person = 10^20 bp/Earth
 * DNA synthesis
 * 1) Deprotect
 * 2) Coupling
 * 3) Oxidation
 * 4) Capping