IGEM:MIT/2009/PCB Spirulina

The resulting spectrum received from the spec is shown:



Here is an example of what is a good read out from the Quail lab.



The concentration is found by this formula:

$$C(mM)=\frac{A_{680}}{37.9}*\begin{cases} 100 (for 1:100) \\ 1000 (for 1:1000) \end{cases}$$

We didn't trust the pathlength correction given to us by this spec so we ran the 1:100 and 1:1000 dilutions in the Stephanopolous lab, and here are the results that we got:

1:100 - A680 = 1.208 1:1000 - A680 = .425

After completing the calculation with the 1:1000 dilution, we got a concentration of 11.21372 mM, and we have a 4.5 mL solution.