IGEM:metu/2009/Notebook/wound dressing/2009/09/03

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03.09.2009
1. We couldn't make isolation.

2. Electrophoresis order; 1)leader / c1/ 1a / 1b / c2 / 2a / 2b / c4 / 4a / 4b /empty / 8a / 8b /c8

3. Digestions of 1,2 and 4 were made. The following amounts are added to microtubes; 1a                                  1b 1,        Buffer                  4 µl                                 4  µl Spe1                   4 µl                   EcoR1         4  µl DNA                    28 µl                                27 µl water                  4 µl     =40 µl                      7 µl       =42 µl

2a                                  2b 2,        Buffer                  4 µl                                 4  µl Pst1                   4 µl                   Xba1          4  µl DNA                    28 µl                                26.5 µl water                  4 µl     =40 µl                      7.5 µl     =42 µl

4a                                  4b 4,        Buffer                  4 µl                                 4 µl Spe1                   4 µl                   EcoR1         4 µl DNA                    27 µl                                25 µl water                  7 µl     =42 µl                      9 µl       =42 µl

4. We coldn't digest.