IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/08/Zhangs

Transformation for the ligation product: T7p+2M/1H/11N/2G/2I/5N/1J RBS+CI+1-23L, 7 samples in total.

Double digestion for 1-2K+CI+1-23L insert again (yesterday failed)

Electrophoresis and Gel Extraction for 1-2K+CI+1-23L and CI+1-23L insert (for 1-5J vector)

Ligate the 1-2K+CI+1-23L insert and T7 promoter vector System

Ligate the CI+1-23L insert and 1-5J RBS vector System