User:Tara K. Luckau/Notebook/Team ConGen/2010/11/09

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Meeting with Rulon - PCR fixes

 * 1) try Scun22 primers
 * 2) * maybe Scun2 just doesn't cross-amplify between Sceloporus undulatus and Sceloporus occidentalis
 * 3) check math on dNTPs, ladder (too bright?)
 * 4) * maybe I didn't make them correctly
 * 5) check pH of Tris-Cl and Low TE
 * 6) * the current working stocks of both are being stored in 50mL conicals instead of glass
 * 7) try positive PCR product straight into gel
 * 8) * is my ladder too concentrated or is my DNA not amplifying
 * 9) PCR Rulon's timber rattlers
 * 10) * Box "C. horridus Primer stocks"
 * 11) ** use #9 (needs rehydration)
 * 12) ** use his Plat Taq, 15mM MgCl2, 10x PCR Buffer, dNTPmix
 * 13) * Box "C. horridus NH DNA extract 9-17-8"
 * 14) ** extracted DNA, but from different locale
 * 15) ** NanoDrop before using (don't know concentrations)
 * 16) try better quality Taq
 * 17) * may need higher fidelity, or ice wasn't enough


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