The BioBricks Foundation:Standards/Technical/BBF WetLab Challenges

=BBF WetLab Challenges=

Vincent 05:05, 11 February 2008 (CST): I tried to summarize the ongoing discussion. Feel free to edit as you like.

Suggested Mission Statement
To bring the BBF community together ,on a regular basis, to support and challenge the establishment of wet-lab related standards in the area of biological engineering.

Benefits

 * Build critical mass to generate valuable experimental data
 * Test reliability and reproducibility of proposed Standards
 * Foster BBF community

Targeted Audience

 * BBF enthusiasts
 * iGEM students

Possible topics

 * Standard Calibration/Measurement procedures:
 * Endy:Measuring_PoPS_on_a_plate_reader
 * Standardized_GFP_quantification
 * Characterisation of basic parts: (Promoters, terminators, RBS, Inverters)
 * Registry/Measurement_kit
 * Endy:Screening_plasmid
 * pLac / pBad promoters
 * Distributed 'Design of Experiment' on a specific device/system (for sensitivity/robustness analysis)

Suggested workflow

 * 1) Define a list of parts ,or protocols, to be challenged/characterized --online--
 * 2) BFF community vote for the top priority (doodle type) --online--
 * 3) Define a common protocol to follow on the day --online--
 * 4) Define how the data are going to be stored/shared for future analysis --online--
 * 5) Organize wet lab session --face to face--
 * 6) Collect data and share them (take pictures and video) --face to face--
 * 7) Analysis and discussion of the outcomes --online--


 * 1) a new cycle starts ...

Practical Issues

 * 1-2 days activities (week-end type of events), it is a constrain for some experimental work.
 * Every 4-6 months
 * Centralized versus Decentratized wet lab organization
 * Vincent 05:05, 11 February 2008 (CST): I would prefer centralized as it helps to build community spirit, at least regional-type of gathering.
 * Storing and Sharing data generated: Where ? How ? Curation ?
 * find a catchy name for the event

Resources

 * Discussion started on the Technical Standard Mailing List, Feb 2008.
 * Technical Resources
 * Similar type of event in other areas:
 * Hackathon / Sprint (from Wikipedia)
 * Google Summer of Code
 * Maker Faire