User:Raf Aerts/Notebook/Open Coffee/2009/02/24

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon_coffee.png|128px]] Open Coffee
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Coffea arabica DNAx :: lysis buffers C0/C1

 * Nanodrop ND-1000 Spectrophotometer setup
 * nucleic acid DNA-50
 * blank: 1.5 μL CE buffer
 * samples 1.5 μL


 * Results
 * C0 buffer gives high peak near 230 in visual reference graph
 * C1 buffer shows no peak at all
 * Both C0 and C1 yield low concentration, low purity DNA (all 260/280 and 260/230 < 1.8)
 * C0: 32.7 ng/ul
 * C1: 8.5 ng/ul




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