IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/10

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM iGarden
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Team Flavor

 * Colonies from the Miraculin/Brazzein-YFP-NOSt+STOP constructs were placed in 5 mL LB AMP media and left in the 37°C shaker.
 * Plated bacteria was left for storage at 4°C

Team Allergy

 * PCRd pHAN.pdk, pKAN.pdk (they're the same 741bp pdk) using the values that worked in the gradient, got them in high ODs. Put them in box a2 rows 3-4.

Innoculating colonies from Friday's ligations

 * Barnase, Gal4, LacIN
 * 4 cc tubes each, 5mL LB+amp, set to shake at 37°C overnight