Endy:Victor3 counteracting evaporation in long time courses

Also see: Victor3 plate reader

Source

 * Rosenfeld et al. J. Mol. Biol. (2002) 323, 5, pp785-93.

Protocol

 * Add an injection step into the Victor protocol to inject 12ul of DI water into each well every 25mins if doing an experiment. Or add water at any time interval that leads to a rate of .48ul/min.
 * I believe this is a slightly faster rate of addition than used in the article above.
 * Note: Check the water reservoir before beginning the protocol to ensure that there is sufficient water for the length of your protocol. 96 wells, measured over 24 hours, will require slightly more than 50 mL.

Calculating time between dispensing steps

 * To calculate the delay time between dispensing steps, it helps to know the time periods of other common steps during the run.
 * To read the absorbance and fluorescence of all 96 wells, it takes 2 mins.
 * To dispense 5&mu;L in each of the 96 wells, it takes 4 mins.