Library Construction Strain Box 1

All in MC4100, pSB1A2 unless otherwise specified.


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! 1 ! 11 ! 21 ! 31 ! 51 ! 61 ! 71 ! 81 ! 91
 * || CW25553 || pJAT8 || BL21(DE3) || pLysS || I13401 || I13401 (4A2) || I13401 (2K3) || I13401 (3K3) ||
 * || I13402 (2K3) || I13403 (1AK3) || I13404 || I13405 || || I13407 || I13408 || ||
 * || I13410 (1AK3) || || I13412 || I13413 || || || || ||
 * || I0500 (2K3) || I6300 (DH5a) || I6301 || Q04400 (2K3) || I15015 || I15016 || I15017 || ||
 * || I13404 (DH5a) || I13405 (DH5a) || I13450 (1A3) || I13452 (1A3) || || || || ||
 * || I13500 || || I13502 || I13503 || I13504 || I13505 || || ||
 * }
 * }


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!colspan="3"|Number of Transformations !Reagents||1||5 (6X)||10 (11X)
 * PEG 3500 50% w/v||240 µl||1440 µl||2640 µl
 * LiAc 1.0 M||36 µl||216 µl||396 µl
 * Boiled SS-carrier DNA||50 µl||300 µl||550 µl
 * Plasmid DNA plus Water||34 µl||204 µl||374 µl
 * Total||360 µl||2160 µl||3960 µl
 * }
 * Plasmid DNA plus Water||34 µl||204 µl||374 µl
 * Total||360 µl||2160 µl||3960 µl
 * }
 * Total||360 µl||2160 µl||3960 µl
 * }

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