Registry/Measurement kit/Notebook/2007-6-13

Registry/Measurement_kit/Notebook

Colony PCR

 * 1-3: #1 ( E0240 ) -- #3,size OK
 * 4-6: #2 ( R0040 ) -- #6,size OK
 * 7-9: #3 ( I13401 ) -- #9,size OK (sort of, 7&8 are slightly smaller)
 * 10-12: #4 (#12 bad) ( J04650 ) -#11, size OK
 * 13: #5 ( B0032 ) -- #13,size OK


 * note: reagents in PCR TOOLS box in fridge, Taq is in Shared polymerase in the fridge near entrance

Analytical gel

 * lane 1: ladder empty
 * lanes 2-13: samples as above
 * lane 14: ladder
 * lanes 15-18: jason's stuff



 2 log ladder

Overnight cultures

 * inoculated 5mL of LB+5µL of Amp. with 20µL of cell suspension from the colonies denoted in section 1.