User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/18

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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To do

 * restriction digest of A. vaga
 * amp AV genes

Primer sequences for A vaga alien genes identified by Gladyshev

 * the last two pairs amplify a 3kb intergenic fragment in one of the collinear pairs (hsp82 region)

looking for alien genes in vaga and ricciae

 * 3m 94C (10s 94C; 3m 60C)x35
 * made 2 big 1% gels with 80ml TAE
 * loaded 2ul vaga followed by ricciae, left to right


 * maybe try an RsaI or Alu1 digest of long fragment for kicks
 * sequence AV10016 (6th from end) and AV10027 (8th from end)
 * both located on EU643475[accn]

trying restriction enzymes on A. vaga and ricciae

 * 37C for 2 hours
 * vaga, then ricciae in the following order: