User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/26

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] ELISA & Counting splitting cells
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Short summary

ELISA

 * Same actions as 18Feb2010

Results

 * HT4 cells
 * 286
 * 249
 * 203
 * 193
 * D12 hTERT cells (15.75×104 cells/mL)
 * 20
 * 12
 * 15
 * 16
 * S+ 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL)
 * D12 P28 of Mark hTERT cells (75.5×104 cells/mL)
 * 92
 * 67
 * 83
 * 60
 * 0.43 mL cells in 23 mL for 24-wells, 3.97 mL in 15 mL for 6 wells
 * D9 (53.5×104 cells/mL)
 * 52
 * 55
 * 64
 * 58
 * 0.61 mL in 13 mL and 5.35 mL in 15 mL


 * ELISA

Conclusion

 * ✅ Ow..

Run 2: Ht31 Dose/Effect Curve & S0 D9/D12

 * Counting cells 19Feb2010
 * Putting to S0 23Feb2010
 * Stimulating cells 24Feb2010
 * Ending stimulation & Viability 25Feb2010

Same action

 * ELISA
 * Counting splitting cells
 * Counting splitting cells

Attachment
   [[Media:HTERT_Donor_9_Ht31_010310_-_dose_effect_curve.xls| Excel Dose Effect curve D9]]    [[Media:HTERT_Donor_12_Ht31_010310_-_dose_effect_curve.xls| Excel Dose Effect curve D12]]


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