User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/02

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Put cells to S0, Splitting cells, stopping stimulation
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Put ELISA plates to S0
 * Split cells for ELISA, IF (310700 cells/mL)
 * 24-wells for ELISA, 20.000 cells per well (0.966 mL cells + 14.034 mL S+)
 * 35mm dishes for IF, 100.000 cells per well (5.150 mL cells + 10.850 mL S+)
 * 35mm dishes for IF, 75.000 cells per well (3.862 mL cells + 12.138 mL S+)
 * IF: CTR/CSE, CTR+DMSO/CSE+DMSO & CTR+Ht31/CSE+Ht31
 * Stop stimulation and Alamar Blue

Results

 * All cells treated with CSE are dead, CSE was too strong??


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