IGEM:Peking/2007/Switch-Notebook/2007-7-27

Re-digestion of plx007, plx008 and plx009
Plasmid 2uL

XhoI 0.5uL

H buffer 2uL

ddH2O 15.5uL

electrophoresis result: invisible

Re-PCR of LacZa and CD2, CD3
primer-F/R 1uL each

Ex-Buffer 5uL

dNTP 5uL

Ex-Taq 0.25uL

ddH2O 36.75uL

60 degrees and 35 cycles with temperature grad

==single digestion of plx007, 008, 009 Plasmid 4uL

XhoI 0.5uL

H buffer 2uL

ddH2O 15.5uL

miniprep of lacZa-T using positive transformation colony and digestion
plasmid 10uL

XhoI/SalI 0.5uL

H buffer 2uL

ddH2O 7uL

total 20uL

digestion for 1 hour and electrophoresis test for the result.