User:Daniel Goodman/Notebook/Cluzel/2010/03/05

Grow cells

 * Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM)

Made agarose

 * Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM)

Make chip impression in solidified gel

 * Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down?

Results: no features found with significant 'push' on 3% gel.

Try transferring again (w/ features)

 * Scope access at 4PM
 * Start gel pouring at 2:30 - Poured 3% at 2:30, poured 5% at 2:50

While waiting...

 * Play with a few featureless gels, practice flipping & transferring


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