Endy:Victor3 Calculating fluorescent protein synthesis

Background subtraction
Subtract a media background, $$A_{media}$$, from the raw absorbance data, $$A_{raw}$$, and assume that the resulting data, $$A_{corrected}$$, is directly proportional to the number of cells in the well. Subtract a fluorescent protein-free cell background, $$G_{cells}$$, from the the raw fluorescent data, $$G_{raw}$$, and assume that the resulting data $$G_{corrected}$$ is proportional to the total number of GFP molecules in the well [immature GFP?].

Unit conversion
Use standard calibration curves (see here for absorbance and here for fluorescence) to convert the background-corrected data into absolute units (CFU/well and GFP molecules per well). The calibration equations used are shown in Equations 3 & 4.

GFP synthesis rate calculations
To calculate the mean synthesis rate of GFP per cell, $$S_{cell}$$, assume the total GFP synthesis rate is equal to the time differential of $$GFP$$. $$S_{cell}$$ can be calculated as the total synthesis rate divided by $$CFU$$.