User:Tina Luetke-Eversloh

contact info
Dr. Tina Lütke-Eversloh

University of Rostock Institute of Biosciences Microbiology Albert-Einstein-Str. 3 18051 Rostock Germany

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research interests

 * Genetic engineering of Clostridium acetobutylicum
 * Physiology and regulation of clostridial solvent formation
 * Metabolic engineering for improved biofuel production

curriculum vitae
UNIVERSITÄT ROSTOCK MASSACHUSETTS INSTITUTE OF TECHNOLOGY, CAMBRIDGE WESTFÄLISCHE WILHELMS-UNIVERSITÄT, MÜNSTER STÄDTISCHES GYMNASIUM LAURENTIANUM, ARNSBERG
 * since July 2007: Junior research group leader at the Institute of Biosciences, Division of Microbiology
 * June 2007: Return grant funded by the Deutsche Forschungsgemeinschaft
 * June 2004 – May 2007: Postdoc at the Bioinformatics and Metabolic Engineering Laboratory, Department of Chemical Engineering
 * June 2004 – May 2006: Research fellowship funded by the Deutsche Forschungsgemeinschaft
 * August 2003 – April 2004: Postdoc at the Institute of Molecular Microbiology and Biotechnology
 * March 30, 2004:	Award of the "VAAM Promotionspreis" (PhD thesis prize, granted by the German Society of General and Applied Microbiology)
 * July 22, 2003: PhD graduation (grade: "summa cum laude")
 * August 1999 – July 2003: Research assistant at the Institute of Molecular Microbiology and Biotechnology and experimental PhD thesis entitled "Microbial synthesis and biotechnological production of polythioesters"
 * June 28, 1999: "Diplom" certificate (MSc graduation; grade: "sehr gut")
 * May 1998 – June 1999: Experimental diploma thesis entitled "Biochemical and molecular genetic studies on the metabolism of 4-hydroxybutyric acid in Ralstonia eutropha" at the Institute of Microbiology
 * October 1993 – June 1999: Studies of Biology
 * June 15, 1993: "Abitur" certificate (High school graduation; grade: 2.5)

publications
ORIGINAL PUBLICATIONS REVIEW ARTICLES INTERNATIONAL PATENTS
 * Lütke-Eversloh, T. & Stephanopoulos, G. (2008). Combinatorial pathway analysis for improved L-tyrosine production in Escherichia coli: identification of enzymatic bottlenecks by systematic gene overexpression. Metab. Engin. 10, 69-77.
 * Lütke-Eversloh, T. & Stephanopoulos, G. (2007). A semi-quantitative high-throughput screening method for microbial L-tyrosine production in microtiter plates. J. Ind. Microbiol. Biotechnol. 34, 807-811.
 * Lütke-Eversloh, T. & Stephanopoulos, G. (2007). L-Tyrosine production by deregulated strains of Escherichia coli. Appl. Microbiol. Biotechnol. 75, 103-110.
 * Impallomeni G., Steinbüchel, A., Lütke-Eversloh, T., Barbuzzi, T. & Ballistreri, A. (2006). Sequencing Microbial Copolymers of 3-Hydroxybutyric and 3-Mercaptoalkanoic Acids by NMR, Electrospray Ionization Mass Spectrometry, and Size Exclusion Chromatography NMR. Biomacromolecules 8, 985-991.
 * Wübbeler, J. H., Lütke-Eversloh, T., Vandamme, P. VanTrappen, S. & Steinbüchel, A. (2006). Tetrathiobacter mimigardefordensis sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3'-dithiodipropionic acid. Int. J. Syst. Evol. Microbiol. 56, 1305-1310.
 * Lütke-Eversloh, T. & Stephanopoulos, G. (2005). Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli: generation and characterization of tyrosine-insensitive mutants. Appl. Environ. Microbiol. 71, 7224-7228.
 * Kim, D. Y., Lütke-Eversloh, T., Elbanna, K., Thakor, N. & Steinbüchel, A. (2005). Poly(3 mercaptopropionate): A non-biodegradable biopolymer? Biomacromolecules 6, 897-901.
 * Thakor, N., Lütke-Eversloh, T. & Steinbüchel, A. (2005). Application of the BPEC pathway for large-scale biotechnological production of poly(3-mercaptopropionate) by recombinant Escherichia coli, including a novel in situ isolation method. Appl. Environ. Microbiol. 71, 835-841.
 * Lütke-Eversloh, T., Elbanna, K., Cnockaert, M. C., Mergaert, J., Swings, J., Manaia, C. M. & Steinbüchel, A. (2004). Reclassification of Caenibacterium thermophilum as Schlegelella thermodepolymerans. Int. J. Syst. Evol. Microbiol. 54, 1933-1935.
 * Elbanna, K., Lütke-Eversloh, T., Jendrossek, D., Luftmann, H. & Steinbüchel, A. (2004). Studies on the biodegradability of polythioester copolymers and homopolymers by polyhydroxyalkanoate (PHA) degrading bacteria and PHA depolymerases. Arch. Microbiol. 182, 212-225.
 * Elbanna, K., Lütke-Eversloh, T., Van Trappen, S., Mergaert, J., Swings, J. & Steinbüchel, A. (2003). Schlegelella thermodepolymerans gen. nov., sp. nov., a novel thermophilic bacterium that degrades poly(3-hydroxybutyrate-co-3-mercaptopropionate). Int. J. Syst. Evol. Microbiol. 53, 1165-1168.
 * Lütke-Eversloh, T. & Steinbüchel, A. (2003). Novel precursor substrates for polythioesters (PTE) and limits of PTE biosynthesis in Ralstonia eutropha. FEMS Microbiol. Lett. 221, 191-196.
 * Liu, S.-J., Lütke-Eversloh, T. & Steinbüchel, A. (2003). Biosynthesis of poly(3-mercaptopropionate) and poly(3-meraptopropionate-co-3-hydroxybutyrate) with recombinant Escherichia coli. Chin. J. Biotechnol. 19, 195-199.
 * Kawada, J., Lütke-Eversloh, T., Steinbüchel, A. & Marchessault, R. H. (2003). Physical properties of microbial polythioesters: Characterization of poly(3-mercaptoalkanoates) synthesized by engineered Escherichia coli. Biomacromolecules 4, 1698-1702.
 * Lütke-Eversloh, T., Fischer, A., Remminghorst, U., Kawada, J., Marchessault, R. H., Bögershausen, A., Kalwei, M. Eckert, H., Reichelt, R., Liu, S.-J. & Steinbüchel, A. (2002). Biosynthesis of novel thermoplastic polythioesters by engineered Escherichia coli. Nature Materials 1, 236-240.
 * Lütke-Eversloh, T., Kawada, J., Marchessault, R. H. & Steinbüchel, A. (2002). Characterization of biological polythioesters: Physical properties of novel copolymers synthesized by Ralstonia eutropha. Biomacromolecules 3, 159-166.
 * Ewering, C., Lütke-Eversloh, T., Luftmann, H. & Steinbüchel, A. (2002). Identification of novel sulfur-containing bacterial polyesters: biosynthesis of poly(3-hydroxy-S-propyl-ω-thioalkanoates) containing thioether linkages in the side chains. Microbiology 148, 1397-1406.
 * Lütke-Eversloh, T., Bergander, K., Luftmann, H. & Steinbüchel, A. (2001). Biosynthesis of poly(3-hydroxybutyrate-co-3-mercaptobutyrate) as a sulfur analogue to poly(3-hydroxybutyrate) (PHB). Biomacromolecules 2, 1061-1065.
 * Lütke-Eversloh, T., Bergander, K., Luftmann, H. & Steinbüchel, A. (2001). Identification of a new class of biopolymer: bacterial synthesis of a sulfur containing polymer with thioester linkages. Microbiology 147, 11-19.
 * Lütke-Eversloh, T. & Steinbüchel, A. (1999). Biochemical and molecular characterization of a succinate semialdehyde dehydrogenase involved in the catabolism of 4-hydroxybutyric acid in Ralstonia eutropha. FEMS Microbiol. Lett. 181, 63-71.
 * Lütke-Eversloh, T., Santos, C. N. & Stephanopoulos, G. (2007). Perspectives of biotechnological production of L-tyrosine and its applications. Appl. Microbiol. Biotechnol. 77, 751-762.
 * Lütke-Eversloh, T. & Steinbüchel, A. (2004). Microbial polythioesters. Macromol. Biosci. 4, 165-174.
 * Lütke-Eversloh, T. (2004). Mikrobielle Synthese und biotechnologische Produktion von Polythioestern. BIOspektrum 4, 416.
 * Steinbüchel, A. & Lütke-Eversloh, T. (2003). Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochem. Engin. J. 16, 81-96.
 * Lütke-Eversloh, T. & Steinbüchel, A. (2002). Polythioesters. In: Biopolymers Vol. 9 (Steinbüchel, A. & Matsumura, S., Eds.), Wiley-VCH Weinheim, pp. 63-80.
 * Lütke-Eversloh, T. & Stephanopoulos, G. N. (2005). Mutations for enhanced tyrosine production. WO 2006/124895.
 * Lütke-Eversloh, T., Steinbüchel, A. & Ewering, C. (2000). Sulfur containing polyhydroxyalkanoate compositions and method of production. WO 2002/16627.

links

 * Mikrobiologie Rostock
 * Stephanopoulos Lab
 * IMMB Münster