IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/06/24/Rencheng Gao

16:21 Double-enzyme digestion: BtoB plasmid (090623) with EcoRI and PstI

16:30 Pick Colony 1 from the plate of BtoB (090623) and cultivate the bacteria in LB medium with Kan.

20:00 Gel electrophoresis: the product of double-enzyme digestion.

20:15 Double-enzyme digestion: BtoB plasmid (090623) with EcoRI and PstI