User:Vijay/Notebook/2007-2-19

Targetron msa knockout

 * Plasmid prep from msa/control, RN4220 and Dh5
 * Gel shift
 * 4220 didnt work out
 * Dh5 worked out, all colonies picked had the plasmid

Transformation

 * Used the Dh5 derived retargeted plasmed to transform 4220
 * Plated both msa/control on erm, incubated at 32oC

Cell wall

 * Isolate cell wall from wt/mutant
 * Cells grown overnight in TSB, without antibiotics at 225 rpm and 37oC