User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Immunofluorescence for cells, Gravin & RII localization
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Stopped CSE treatment and started immunofluorescence protocol
 * cells: D9 P25
 * Primary antibody both diluted 1:1000 in the same suspension
 * RIIα (612243 BD Bioscience)
 * Gravin (antibody ZDE10030 animal 122 antibody (Eurogentech antibody service))
 * Secondary antibody diluted 1:300 and DAPI staining (50x) diluted 1:50
 * NOTE: They might have been too long in dH2O
 * Finished PKA Co-IP and added 50 μL of NuPage loading buffer + DTT (stored @ -20°C)
 * Split cells D9 P26 to P27


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