IGEM:PennState/2007/Progress Cloning

Primers: Promoter Region Primers

Double Promoter

Full Promoter AtoF F-GGAATTCGCGGCCGCTTCTAGAGTATTGAACTCCATAATCAGGTAATG R-CTGCAGCGGCCGCTACTAGTATGGTGTAGGGCCTTCTGTAG
 * TmC 61
 * TmC 62

Full Promoter FtoA F-GGAATTCGCGGCCGCTTCTAGAGTGGTGTAGGGCCTTCTGTA R-CTGCAGCGGCCGCTACTAGTATATTGAACTCCATAATCAGGTAATG
 * TmC 61
 * TmC 58

ORF Promoter AtoF
 * MISSING**

ORF Promoter FtoA F-GGAATTCGCGGCCGCTTCTAGAGAACCAATTTTTAGCAACTAAACAG R-CTGCAGCGGCCGCTACTAGTAATGGATGATGTCGTAATATTGG
 * TmC 58
 * TmC 59

Single Promoter

CRP XylR XylR Fis F- GGAATTCGCGGCCGCTTCTAGAGTCCCTTTCAGTTGCTCAAT R- CTGCAGCGGCCGCTACTAGTAGGTGTAGGGCCTTCTGTAG
 * TmC 58
 * TmC 60

XYLR XYLR FIS F-GGAATTCGCGGCCGCTTCTAGAGTTACGTTTATCGCGGTGATT R-CTGCAGCGGCCGCTACTAGTAGGTGTAGGGCCTTCTGTAG
 * TmC 59
 * TmC 60

CRP F-GGAATTCGCGGCCGCTTCTAGAGATGGTGCTTGGCAAACC R-CTGCAGCGGCCGCTACTAGTATTAACGAGTGCCGTAAAC
 * TmC 60
 * TmC 57

XylR

XylR with Promoter RBS and terminator: Right F-GGAATTCGCGGCCGCTTCTAGAGGCAACCAAACGCCGTTCTTG R-CTGCAGCGGCCGCTACTAGTAATTGCATTTCCTTGAGCCTTATCC
 * TmC 65
 * TmC 63

XylR with Promoter RBS and terminator: Left F-GGAATTCGCGGCCGCTTCTAGAGATTGCATTTCCTTGAGCCTTATCC R-CTGCAGCGGCCGCTACTAGTAGCAACCAAACGCCGTTCTTG
 * TmC 63
 * TmC 65

XylR ORF Right
 * Missing**

XylR ORF Left
 * Missing**