AG Daniel:Freezer

Klenow fragment
Klenow fragment

- GoTaq® Polymerase
GoTaq

- InviTaq Polymerase
InviTaq

- DreamTaq™ Green DNA Polymerase
10x DreamTaq™ Green Buffer 5 µl dNTP Mix, 2mM each         5 µl (0.2 mM of each) Forward primer             0.1 - 1.0 µM Reverse primer             0.1 - 1.0 µM Template DNA               10 pg - 1 µg DreamTaq™ DNA Polymerase   1.25 u     Water, nuclease-free        to 50µl Total Volume 50µl

Shrimp Alkaline Phosphatase
Phosphatase catalyzes the removal of 5´ phosphate groups from DNA and RNA. Since phosphatase-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.

USB SAP #E70092Y

T4 DNA Ligase
NEB T4 Ligase

TOPO® TA cloning kit
Invitrogen TOPO cloning

Restriction Enzymes
(status: 02/2010)