IGEM:PKU Beijing/2009/Notebook/AND Gate 2/2009/07/27/Zhenglin Yang

11:00 Double digestion of purified T7 polymerase PCR production

12:30 Tube cultivation of T3 promoter + GFP

14:30 Tube cultivation of T3 promoter parts

19:30 Tube cultivation of T3 Polymerase

21:00 Purify the digestion production of T7 polymerase

22:30 Ligation of T7 polymerase and 1-2M vector