JCA061306-Construction of pSB1A2-J23000

 [pMB1][AmpR][StrRBS][TraJF]+[dblTerm] digest pSB1A2-J01052	(SpeI/AlwNI, Neb2, Large-2241) digest pSB1A2-B0015	(XbaI/AlwNI, Neb2, Small-546) Ligate, Product pSB1A2-J23000 