JCA080306-COnstruction of pSB3C6-Bca1025 = pSB3C6-J23045

 [Ptet][StrRBS]-X-[GFP(AGGA)]-[p15A][CmR] N	998	1025R	pBca1021-E0040	580bp O	1025F	G00101	pBca1021-E0040	534bp P	998	G00101	N+O	1118bp

P is the overlap PCR of N and O, sub EcoRI/PstI into pAC997	(EcoRI/PstI) Product is pSB3C6-Bca1025 = J23045

ca1025F	Forward quickchange for GFP[S56AGGA] gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac ca1025R	Reverse quickchange for GFP[S56AGGA] gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc 