User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/06/15

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June 15th, 2010
1. Prepare culture dishes with Ampicilin antibiotic.
 * Instructions are as indicated on April 20th.

2. PCR with the minimum Blue Promoter and suffix primers to amplify plasmid 30 and ligate Blue Promoter to this plasmid.
 * Reactives needed per sample:
 * PCR with RTTH polymerase
 * Reaction 1
 * HPLC -> 10
 * Buffer 3.3x -> 6
 * Mg(Ac)2 -> 3
 * dNTP’s	-> 2
 * Primer FWD -> 2.5
 * Primer REV -> 2.5
 * DNA -> 4
 * Total -> 30


 * Reaction 2
 * HPLC -> 10.5
 * Buffer 3.3x -> 9
 * RTTH -> 0.5
 * Total -> 20


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