Silver: Protocols

See also the OpenWetWare Protocols Collection.

Silver Lab Members: Link to your protocols from both this page and the OpenWetWare Protocols page.

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Genome Organization

 * S. cerevisiae Protocols
 * ChIP-chip
 * FISH/IF
 * Immunofluorescence
 * LiOAc Transformation
 * Propidium Iodide FACS
 * Mammalian Protocols

RNA

 * Poly A RNA in situ protocol
 * RNA Export Assay

Synthetic Biology

 * Strategy for Making Parts
 * Commonly Used Plasmids
 * Commonly Used Primers
 * PCR
 * Oligonucleotide Inserts
 * Overlapping Oligonucleotide Inserts
 * Site-Directed Mutagenesis
 * Restriction Digest
 * Ligation
 * Quick & Dirty Digest & Ligation
 * Bacterial Transformation
 * Plasmid Verification
 * Integration into Yeast
 * Verifying Genomic Integration

Cell-Based Screens

 * Chemical compound screens
 * miRNA screens
 * High-throughput immunostaining
 * FKHR Translocation Assay

Methylation

 * Methylation Assay
 * In Vivo Protein Methylation Assay (S. cerevisiae)

S. cerevisiae Protocols

 * Lysate for Western
 * Colony PCR
 * Time-Lapse Microscopy

Mammamlian Tissue Culture Protocols

 * Colony picking using cloning rings

Other Protocols

 * Bioptechs live-cell micro-observation perfusion chambers
 * Cleaning Glass Beads

Common Reagents

 * Ampicillin
 * B Plates
 * Coomassie Stain
 * Destain
 * Dropout Media
 * Dropout Plates
 * 0.5 M EDTA pH 8.0
 * 20% Glucose
 * 50% Glycerol
 * LB Liquid
 * LB-Amp Plates
 * 10x PBS
 * Protease Inhibitors
 * ssDNA
 * SOC
 * 50x TAE
 * 1M Tris Base
 * TE
 * YEPD Liquid
 * YEPD Plates

Hardware_Maintenance

 * Microscope