Davidson:The What's and How's

loxP sites Hin/Hix CRE gene Degradation Tags
 * What is the list of DNA parts that we will need for the first stage, second stage, entire project?
 * ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
 * ERIN and SABRIYA Which DNA parts will have to be designed? Will they be synthesized or produced by PCR?


 * 1) Common molecular reagents
 * 2) Standard Assembly
 * 3) Compatibility of Plasmids
 * 4) Building dsDNA with Oligos
 * 5) Setting up PCR mixtures
 * 6) PCR and Mg2+ concentration
 * 7) Clean and Concentrate DNA (after PCR, before digestion)
 * 8) Pouring an agarose gel
 * 9) Calculate MWs
 * 10) Digest DNA with restriction enzymes
 * 11) Double Digest Guide
 * 12) Ethanol Precipitate DNA (short protocol)
 * 13) 1kb MW markers
 * 14) Shrimp Alkaline Phosphatase
 * 15) Qiagen QIAquick Gel Purification
 * 16) Qiagen QIAquick Column Regeneration Protocol
 * 17) ElectroElute Gel Purification
 * 18) Ligation Protocol
 * 19) Heat Shock Transformation OR Short version of Heat Shock
 * 20) Zippy Transformation
 * 21) Colony PCR to Screen for Successful Ligations
 * 22) Promega miniprep
 * 23) Choices for Transformation: Heat Shock vs. Zyppy
 * 24) Choices for Mini-Preps: Promega vs. Zyppy