User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/09

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Halometabolites from uncultured bacteria
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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TAR cloning progress

 * Clone ZB118 targeted with US and DS sequences (ABC transporter selected before NRPS system in ZB118).
 * PCR products indicate successful amplification with Buffer G at 65C for 30 cycles. (1, 2kb products see gel)
 * pLLX8 amplificaiton with TAR primers (ala Lory) look fine as well. Product is a bit dirty and yield was much lower so I repeated the experiment with a longer extension time (lanes 1 v 2).
 * EcoRI digest of the ten hits from recombinational cloning and assembly of ZB118 capture vector all look good. (released band of expected size, see gel)


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