User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/21

 .todo { color: red } .done { color: green} {| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Lysate SDS-PAGE, qPCR AKAP5, Western blot AKAP79 and Gravin
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Summary

 * SDS-PAGE Lysate samples
 * Forgot antioxidant, added after 1h 11 min.; power failure @ 2h 40 min. → continued to blot (2h, 30V)
 * Ponceau staining does not look very nice compared to others
 * Gel is coomassie stained


 * Prepared cDNA
 * qPCR AKAP5 and GAPDH
 * Not enough master mix for HEK293 and Jurkat controls
 * Western blot 2nd Antibody (α-Rabbit) 1:10.000
 * Blot #1 16August2010, Loading control was already performed 19August2010


 * }