Template:SBB-Protocols Enz1

EcoRI/BamHI Digest of Wobble Products
For wobble products, you will digest the entire extension reaction-worth of DNA Since you started with 50uL of extension reaction, you should now have 50uL of eluted DNA in water. 50uL eluted DNA 5.7uL NEB Buffer 2 0.5uL EcoRI 0.5uL BamHI
 * Set up the following reaction in a PCR tube:
 * Mix thoroughly by slamming the tube upside down on the table, and then a quick spin to move the liquid to the bottom of the tube.
 * Incubate the reaction at 37 degrees on the thermocycler
 * Proceed to another Zymo small fragment cleanup