IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/18

{| width="800"
 * style="background-color: #800000"|[[Image:UIUC_ArsR_System.jpg|150px]] UIUC - Arsenic Collection System!
 * style="background-color: #AFEEEE" align="center"|  |Main project page
 * style="background-color: #AFEEEE" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

8/18/10
11:35 AM

Digestion

ArsRSDM2

B0015

pSB1T3

Incubate at 37 for 15°C min. Inactivate at 80°C for 20 min.

11:50 AM

Ligation

ArsRSDM2-B0015-pSB1T3

Incubate at room temperature for 10 min, then inactivate at 80°C for 20 min.

12:20 PM

Streaked colonies from 8/12/10 plate since I accidentally let them sit at 37°C over the weekend and they may have lost their red color for selection. Streaked 6 colonies from each plate.

3:00 PM 5mL Culture Made 5mL cultures (without antibiotics) of ArsR SDM2 (Amp resistant) and pSB1C3 + LamB transformation from 8/17/10

3:20 PM

Transformation

50 μL cells, 2 μL ligation product. 10 min on ice, 45 sec at 42°C, 2 min on ice. then add 1 mL SOC, recover for 1 hour at 37°C shaking.

4:40 PM

Plated 250 μL cells on Tet plate.


 * }