IGEM:Melbourne/2008/BCRiboswitch/Existing Riboswitches

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From published articles
1. From Issacs et al (3), the crR12-taR12 pair produced the highest cis repression and greatest trans activation. It seems to have been very effective.

1
BBa_J01010 (Lock) “Biobricked version of Isaacs' riboregulator cis repressed lock, crR12”

DNA Availability: Planning

Sequence

BBa_J01060 (Lock) = [pTet][J01010]

DNA Availability: Planning

pTet: constitutively active promoter

Sequence

BBa_J01008 (Key) “BioBricked version of Isaacs' riboregulator trans-activating key, taR12”

Sequence

2
BBa_J01080(Lock)

DNA Availability: Planning

Sequence

BBa_J01122 (Lock) [pTet][J01080][RFP][DblTerm] (Designed by Berkeley 06)

DNA Availability: Planning

Sequence

BBa_J01086 (Key)

DNA Availability: Planning

Sequence

BBa_J23008 (Key) (slightly modified version of J01086)

DNA Availability: [http://partsregistry.org/Part:BBa_J23008 BBa_J23008 Available! ]

Sequence

The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing.

3
BBa_J23066 (Key) (Designed by Berkeley 06)

DNA Availability: [http://partsregistry.org/Part:BBa_J23066 Available! ]

BBa_J23077 (Lock) [Ptet][J23078][RFP][TT]

DNA Availability: Planning

4
BBa_J23070 (Key) (Designed by Berkeley 06)

DNA Availability: [http://partsregistry.org/Part:BBa_J23070 Available! ]