IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/16/Zhangs

Store the spawns of 5N＋CI②~⑤ and 11N+CI③~④. Do the PCR identification of T7P+5N+CI+T②~⑤ and T7P+11N+CI+T③~④ using bacteria liquid; 6 samples in total. Ligate T7P+5J+CI+T again. Miniprep of T7P+5N+CI+T②~⑤; T7P+11N+CI+T③~④ Concentration: 150ng/uL. Electrophoresis to identify the 5N+CI②~⑤ and 11N+CI③~④ PCR products. Result (from left to right) Sample loading order:1-11N+CI③, 1-11N+CI④,1-5N+CI④, 1-5N+CI⑤, 1-5N+CI②, 1-5N+CI③, Marker The PCR identification results show they have been successfully ligated.