User:Anthony Salvagno/Notebook/Research/2010/06/25/Unzipping Construct Ligation

Procedure
I am doing two reactions because I have 2 different concentrations of digested pBR322. According to the nanodrop I have:
 * Gel Extraction from yesterday - ~170ng/ul --> 102nM
 * old gel extraction - ~80ng/ul --> 50nM