IGEM:IMPERIAL/2008/New/Major Results/Calibration Curve1

O.D.600 vs Cell Number Protocol
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Brief Descrption of protocol

 * Prepare an Overnight Culture of B.subtilis
 * Measure the O.D.600
 * Carry out a series of dilutions to give a range of O.D.600 e.g. 0.5, 1, 1.5, 2, 2.5, 3.
 * Carry out dilution plating with each O.D.600,
 * Grow overnight at 37oC ,
 * Count the number of Colony Forming Units,
 * Multiply the dilutions from the dilution plating,

Results

 * We performed the protocol with 8 repeats for each O.D.600 across two dilutions. Examples of the density of plates we counted are shown below.
 * The results of the protocol are summarised in the graph below. To convert from O.D.600 we