IGEM:IMPERIAL/2006/LabCalendar/2006-7-31

Culture Parts for Testing
We will be testing the zinc hypothesis by using the part MIT used in 2004 (I13207). This part as well as T9002 was cultured in 2 mL LB Amp overnight for testing tomorrow. We are thinking of using 4 different tests which will tell us if having zinc in the medium makes all the difference.


 * M9, no Zn, with Arabinose (Control)
 * M9, with Zn, with Arabinose
 * LB, with Arabinose
 * LB, no Arabinose (Control)

M9 medium protocol can be found on OpenWetWare.


 * Also note that to make the Amp LB for Maxi-prep (Midi-prep), go down to level one of Biochem building to the Media Office to order. Bring 500 mL Erlynmeyer flasks and ask for:  100 mL LB with 50 ug/mL Amp.


 * Ligations and part 1I have been minipreped
 * Gel is made ready to be run tomorrow

To do on Tuesday 1 August:
 * 30 uL digests of both 7A and 3O
 * Run large gel to extract DNA fragments
 * For 7A, we want the 756 bp one, NOT 2000 bp fragment
 * For 3O, we want the 2091 bp one, NOT the small fragment
 * Set gel at 130 V, 0.130A to run gel
 * Extract fragments using transilluminator
 * Ligate parts overnight