IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/19/Shan Shen

10:30 Miniprep 2-4O, 1-18P, low-copy bi-stable system Concentration:

12:00 Digest 1-18P, 2-4O, low-copy plasmids. 1-18P by Spe1 and Pst1; 2-4O by Xba1 and Pst1; low-copy bi-stable system by EcoR1 and Pst1.

18:00 Recycle those digestion products.

22:40 Ligation of high-copy bi-stable system fragments and high-copy backbone.