SBB10Ntbk-JennaKelleher2

Labwork
Prepared sbb28 and sbb29 for PCR after creating a master mix with Jeni Lee sbb28 in 2k55 and sbb29 in 55

Updated Constuction Files
Biobricking of 099 rep protein basic part sbb29

PCR repo99-F and JG002-R on pEC52 gene            (978 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144                          (EcoRI/BamHI, 910+2472, L) Product is pBca9523-sbb29	{rbs.repO99!} repo99-F  Cloning of repo99 ccaaaGAATTCatgAGATCTcctggaaggaatcgtaacac JG002-R  Cloning of  O99 rep Gene CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc

Biobricking of CA42 rep gene basic part sbb28

PCR repCA42-F and KRM002 on pEC22-CA42 gene     (1496 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144                          (EcoRI/BamHI, 910+2472, L) Product is pBca9523-sbb28     {5'UTR.repCA42!} repCA42-F  Cloning of repCA42 ccaaaGAATTCatgAGATCTcagctgaagtgaccggattag KRM002  Reverse oligo for cloning of rbs.repCA42! ctgatGGATCCctattttccgcttttccag

Biobricking of pre-pro sequence basic part sbb42

Eco/Bam transfer pBjh1601AC-Bjh1723 Sub into pBjk2741-Bca1144           (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-Bjh1723  	{rbs_pelB>}