IGEM:UNAM/2009/Notebook/IGEM project/2011/05/05

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Medium of Chlamydomonas reinhardtii
Abstract

Today Miriam gave me the protocol to make TAP medium.

Tap Medium

Gauging to 1 liter.

Beijerincks 20X solution

Trace Element Solution

(a) Heat to dissolve (b) Shake to disolve

Dissolve each element independently.

- Boric acid in 200 ml. - Sulphate if zinc in 200 ml. - EDTA in 250 ml. - Other elements in 50 ml.


 * 1. Put all solutions in a flask and add the EDTA, solution has a purple color.
 * 2. Lead to boiling point, until the solution has a green color.
 * 3. Decrease a little the temperature (no less of 70 °C), set pH between 6.5-6.8 with KOH to 20%.
 * 4. Gauge to 1 liter.
 * 5. Leave the solution in a flask until the solution get a purple color.
 * 6. Remove the residue of reddish brown color by filtration.


 * }