User:Emmalee Jones/Notebook/Lab Notebook/2010/04/30

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Finding Even Coverage Concentration

 * Made 0.1 mM total lipid dilution in PEM


 * Used this to make lipid flow cell solution


 * Still not covering the entire slide, so made up a new solution that is 0.5 mM, put in a flow cell


 * This one has even coverage! So that is when I make a 0.5 mM dilution in PEM, then put that into a half and half lipid motility solution for the flow cell.  I only made a half batch of it, and that was plenty to run a few flow cells.

Half batch:

25 μL liposomes 1.25 μL AF    0.5 μL Glucose 23.3 μL PEM