User:Karmella Haynes/Notebook/BioBrick cloning/2010/02/16

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02/16/10

 * &#x2713; Order primers: for BioBrick cloning of DPRE (from CJW)

Primer order > Note: part contains 2 PstI sites > Direct E/S cloning upstream of YFP reporter (ignore PstI's) > PstI mutagenesis primers (make an official BioBrick; to be done in parallel)
 * 1) BBp_DPRE f: 5'-gaattcgcggccgcatctagaCCGAAGAGAAAGGACGCTAA
 * 2) BBs_DPRE r: 5'-ctgcagcggccgctactagtAACCGTGCGGACAGACAGAG
 * 1) mut_DPRE 1: 5'-GAAATGCTcCAGTCCAAGCGGG
 * 2) mut_DPRE 2: 5'-GCGCCACTcCAGATGGCTCACC


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