User:Hassan Rokni Zadeh

Contact Info



 * Hassan ROKNI ZADEH


 * Address: Centre of Microbial and Plant Genetics (CMPG)

Faculty of Bioscience Engineering

K.U.Leuven, Belgium.


 * Email: hassan.roknizadeh@student.kuleuven.be and more information

I work in the Centre of Microbial and Plant Genetics at K.U.Leuven University. I learned about OpenWetWare from google, and I've joined for using protocols, sharing the experiences in biology especially lab skills.

Education
Thesis title: Characterization of antagonistic compounds from plant-associated pseudomonads for biocontrol of Pseudomonas savastanoi, causative agent of olive knot disease.
 * 2007-present, Ph.D student of Agricultural & Applied Biological Sciences, Catholic University of Leuven (K.U.Leuven)

Thesis topic: Vitamin C metabolism study in apple using a        multidisciplinary approach utilizing biochemistry (HPLC) and molecular approaches (Semi-quantitative RT-PCR; the expression pattern of its key     genes) and its response to stress.
 * 2005-2006, Pre-doctoral program in Agricultural & Applied Biological Sciences, Bioscience Engineering Faculty, K.U.Leuven.


 * 2001-2003, M.Sc. (17.95 out of 20), Biology (Plant Genetics), Department of Biology, Shahid Beheshti University, Tehran.


 * 1997-2001, B.Sc., Cell and Molecular Biology (Microbiology), Department of Biology, University of Isfahan, Iran.

Research interests

 * 1) Interest 1: Antibacterial and antifungal compounds as biocontrol use against plant pathogenes.
 * 2) Interest 2 : Plant growth promoting bacteria (PGPR) for biocontrol use
 * 3) Interest 3

Publications

 * 1) Paper1 Rokni Zadeh, H., Khavazi, K., Asgharzadeh, A., Hosseini-Mazinani, M. and De Mot, R. (2008) Biocontrol of Pseudomonas savastanoi, causative agent of olive knot disease: antagonistic potential of non-pathogenic rhizosphere isolates of fluorescent Pseudomonas. Commun. Agric. Appl. Biol. Sci. 73(1) 199-203.


 * 1) Paper2 Rokni Zadeh, H., Keulemans, J., and Davey, MW. (2007) Expression pattern of key vitamin C biosynthesis genes in apple. Commun. Agric. Appl. Biol. Sci. 72(1) 269-73. [[Media:Vitamin_c_paper.pdf|Full text]], PubMed.

unreduced gametes in Gossypium hirsutum L.'' The Nucleus. 47(1,2): 33-37
 * 1) Paper3 Sheidai, M., Shahriari, Z.H., Rokni Zadeh, H. and Noormohammadi, Z. (2007) RAPD and cytogenetic study of some tetraploid cotton (Gossypium hirsutum L.) cultivars and their Hybrids. Cytologia. 72 (1): 77-82. Full text.
 * 2) Paper4 Sheidai, M. and Rokni Zadeh, H. (2004) ''B-chromosomes, cytomixis and

Professional and Computer Skills
Protein, DNA and RNA extraction, cloning, PCR, RT-PCR, Semi-quantitative RT-PCR, RAPD, Box-PCR and RACE, gel electrophoresis, southern blot, microbial and plant tissues (apple and cotton) culture, antagonistic and bacteriocin assay, meiotic and meiosis microscopic preparation, HPLC analysis, clustering methods (UPGMA, WARD...), Statistical analyses, basic knowledge of programming (PERL, JAVA, XML), Biological Database Management, basic knowledge of Bioinformatics (blast, alignment, primer design…).

Job Positions

 * 1) Working on the “identification of QTL’s for vitamin C contents of apple” project, Laboratory of Fruit culture and -biotechnology, K.U.Leuven, Sept.-Oct. 2006.
 * 2) Working on Biosafety protocol, Monsanto Biotechnology Company, Brussels Office, August 2006.

Useful links

 * Iran Olive
 * Centre of Microbial and Plant Genetics