User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/13

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LCO/HCO A.vaga amp
Loaded 2.0ul on Gel



HCO LCO Cycle Sequencing Purification with QIAquick
For A.vaga

http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2


 * see page 19.

HCO/LCO Adenita Culture Cycle Sequencing
For A.vaga

Yeast Culture Prep for Long Term Freezer Storage
From Revived Yeast Culture

1) Overnight culture on agar plate (or grow until sufficient lawn)

Streaked out 50.0ul of growing culture onto a glucose+yeast extract plate

S. pombe - failed to grow - redo S. cerevisiae - good lawn

2) Prepare freezing media = 20% gycerol in sterile YM media. Aliquot into small freezer tube (1000ul).

3) Collect lawn with sterile toothpick and swirl in freezing media

4) Slow freeze in -30C