Erickson:Protocols

PCR
Colony PCR Real-Time PCR PCR Clean-up for Sequencing 2-log Ladder

RNA
RNA Isolation Protocol (TRIzol)

RNA Isolation Protocol (Mini Shredder)

Fleas
Flea Bedding Flea Crushing

Media
LB mixture (includes plates mix) LB Buffer TAE Buffer TB Frozen Stocks

Antibiotics
Kanamycin Carbenacillin Tetracycline Irgasan Chloramphenicol

Transposon Mutagenesis
Tri-parental Mating

Electroporation

Bi-parental Mating

Competent Cell Generation
Competent Cells