IGEM:Harvard/2010/Brainstorming/food

protocols

 * Book: Agrobacterium Protocols

RNAi

 * 1) 1 pmid=10781109

Dual selection system ideas

 * use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
 * cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
 * cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA

De-Greening

 * this paper has some parts that could be useful as kill-switches
 * A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.
 * steroid-inducible chlorophyll knockdown

allergens
Peanut and Tomatoes review
 * 1) 1 pmid=18467156

Peanut profilins: Ara h knock-down
 * 1) 1 pmid=15659267

Tomato profilins: Lyc e knock-down
 * 1) Design of tomato fruits with reduced allergenicity by dsRNAi-mediated inhibition of ns-LTP (Lyc e 3) expression. Le LQ, Lorenz Y, Scheurer S, Fötisch K, Enrique E, Bartra J, Biemelt S, Vieths S, Sonnewald U. Plant Biotechnol J. 2006 Mar;4(2):231-42. pmid:17177799
 * 2) Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants. Le LQ, Mahler V, Lorenz Y, Scheurer S, Biemelt S, Vieths S, Sonnewald U. J Allergy Clin Immunol. 2006 Nov;118(5):1176-83. Epub 2006 Sep 8. pmid:17088146

Orange profilin: Cit s 2 (characterized, but not knocked down yet)
 * 1) Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. López-Torrejón G, Ibáñez MD, Ahrazem O, Sánchez-Monge R, Sastre J, Lombardero M, Barber D, Salcedo G. Allergy. 2005 Nov;60(11):1424-9. pmid:16197476

flavors

 * Growth dependent banana odor generator, MIT iGEM 2006


 * Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde


 * (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid, MIT iGEM 2006


 * phase dependent wintergreen odor generator, MIT iGEM 2006

VANILLA: http://www.3dchem.com/moremolecules.asp?ID=307&othername=Vanilla#


 * 1) 1 pmid=17886091

De novo biosynthesis of vanillin in fission yeast (Schizosaccharomyces pombe) and baker's yeast (Saccharomyces cerevisiae). http://www.ncbi.nlm.nih.gov.ezp-prod1.hul.harvard.edu/pubmed/19286778

miraculin

 * Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce


 * Functional Expression of Miraculin, a Taste-Modifying Protein in Escherichia Coli


 * 1) 1 pmid=3403544

spices
Capsaicin: http://en.wikipedia.org/wiki/Capsaicin


 * 1) 1 pmid=15918882


 * 1) 2 pmid=16938870

From wikipedia:
 * TRPV1, the only known receptor (a transient receptor potential channel) for capsaicin.
 * Piperine, the active piquant chemical in black pepper
 * Allyl isothiocyanate, the active piquant chemical in mustard, radishes, horseradish, and wasabi
 * Allicin, the active piquant flavor chemical in uncooked garlic and onions (see those articles for discussion of other chemicals in them relating to pungency, and eye irritation)
 * Naga Jolokia pepper, the world's most capsaicin-rich fruit
 * Discovery and development of TRPV1 antagonists
 * Capsinoids are similar in structure to capsaicin, but lack the extreme pungency.

others

 * 1) 1 pmid=12940418

http://en.wikipedia.org/wiki/Limonene

caffeine etc.

 * 1) 1 pmid=18068204


 * 1) 2 http://www.plantcell.org/cgi/content/abstract/6/5/723

Questions
can we eat it for the iGEM project (human testing)? Can we hand it out to be eaten (FDA)? Patent/copyright issues? Can we have actual tranformed plants ready by the jamboree