User:Karmella Haynes/Notebook/Polycomb project/2010/05/21

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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05/21/10

 * &#x2713; ChIP: chromatin prep & sonication for KAH126-1 and U2OS samples
 * &#x2713; Western blot: continued from 5/18/10
 * &#x2713; Cell culture: freeze down KAH132 lines (3) (growth assay lines)
 * &#x2713; Protein preps: Pc-ATF neg ctrl lines
 * &#x2713; Growth assay: do cell count (day 4)

Growth assay (day 4) > Do cell counts for the following: --> Checked cells for RFP expression. KAH126-4 shows no RFP. > Trypsinize cells with 200 μL Trypsin buffer > Resuspend cells with 800 μL medium (1 mL total volume) > Dilute samples 1/4 for Scepter counting; add 25 μL cells to 75 μL PBS
 * 1) KAH126-1
 * 2) KAH126-3
 * 3) KAH126-4
 * 4) KAH132-8
 * 5) FTRx
 * 6) KAH126-1 +dox
 * 7) KAH126-3 +dox
 * 8) KAH126-4 +dox
 * 9) KAH132-8 +dox
 * 10) FTRx +dox

> Refresh medium and dox in other wells


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