IGEM:Stanford/2009/Project Homeostasis/sequences/RaldhII

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RBS - RALDH II gene - Terminator gaattcgcggccgcttctagagaaagaggagaaatactagATGCCCGGCGAGGTGAAGGCCGACCCCGCCGCGCTC ATGGCCTCGCTGCAACTCCTGCCGTCACCCACGCCCAACCTCGAGATCAAGTACACCAAGATCTTTATT AACAATGAATGGCAGAACTCAGAGAGTGGGAGAGTGTTCCCTGTCTGTAATCCAGCCACAGGAGAGCAA GTGTGTGAAGTTCAAGAAGCAGACAAGGTGGATATAGACAAGGCAGTGCAGGCGGCCCGCCTGGCCTTC TCTCTTGGTTCTGTGTGGAGAAGGATGGACGCTTCTGAAAGAGGACGTCTGCTGGACAAGCTTGCAGACT TGGTGGAACGGGACAGGGCAACTCTTGCAACTATGGAATCACTAAATGGCGGTAAGCCATTCCTGCAAGC TTTTTACATCGATTTGCAGGGAGTCATCAAAACCCTGAGGTATTATGCAGGCTGGGCTGATAAAATTCACG GAATGACCATTCCTGTAGATGGAGACTATTTTACCTTCACGAGACATGAGCCCATCGGAGTGTGTGGACA GATTATCCCGTGGAATTTCCCCCTGCTGATGTTCACCTGGAAAATTGCTCCCGCACTGTGCTGTGGTAACA CTGTGGTCATCAAGCCGGCAGAGCAGACACCACTCAGTGCACTGTATATGGGAGCCCTCATCAAGGAGGC TGGCTTTCCACCCGGGGTCGTCAACATTCTGCCAGGGTATGGGCCAACAGCAGGGGCAGCAATAGCGTCT CACATTGGCATAGACAAGATTGCATTCACAGGCTCTACTGAGGTTGGAAAGCTTATTCAAGAAGCAGCTGG AAGAAGTAATTTGAAGAGAGTAACTCTGGAACTTGGAGGGAAAAGCCCTAATATTATTTTTGCAGATGCTG ACTTGGACTACGCTGTGGAGCAGGCTCACCAGGGTGTGTTCTTCAACCAAGGCCAGTGCTGCACTGCGGG GTCTCGAATCTTTGTGGAGGAGTCCATCTATGAGGAATTTGTGAAAAGAAGTGTGGAAAGAGCCAAGAGGC GCATTGTGGGGAGCCCCTTTGATCCCACTACTGAGCAGGGTCCTCAGATTGATAAGAAGCAATACAACAAA ATCCTGGAGCTCATCCAGAGCGGTGTGGCCGAGGGCGCCAAGCTCGAGTGTGGAGGCAAAGGACTGGGAA GGAAGGGCTTCTTCATTGAGCCTACCGTGTTCTCCAACGTTACAGATGACATGCGGATCGCCAAGGAGGAG ATCTTTGGTCCTGTCCAGGAAATTTTGAGGTTTAAGACTATGGATGAAGTTATAGAAAGAGCCAATAACTCA GACTTCGGGCTTGTAGCGGCTGTCTTCACTAATGACATCAACAAGGCTCTCATGGTGTCCTCTGCAATGCAA GCTGGGACAGTTTGGATCAATTGTTACAACGCCTTAAATGCTCAGAGCCCCTTCGGGGGGTTCAAGATGTCT GGAAATGGGAGAGAAATGGGTGAGTTTGGCTTACGGGAGTACTCAGAAGTAAAGACCGTGACGGTGAAGAT CCCCCAGAAGAACTCCTAAtactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatactgcagcggccgctactagta
 * Sequence: length= 1732


 * Forward Primer: gtttcttcgaattcgcggccgcttctagaaagaggagaaatactagatgcccgg
 * Length= 54 GC= 50%   Melt= 69.9C


 * Reverse Primer: gtttcttcctgcagcggccgctactagtatataaacgcagaaaggcccacccg
 * Length= 53 GC= 54.7%   Melt= 71C