IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/08

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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June 8, 2010

 * Figured out RBS calculator
 * Drew out assembly plan
 * Consulted with Matt Lux on cassette design
 * Figured out our pre-sequence to use in the RBS calculator
 * Selected a generic terminator sequence
 * Met with the VBI Synthetic Biology Research Group and refined design of biosensor cassette
 * Obtained sequences necessary to test the promoter and submitted to Matt Lux for review
 * Researched and made decision on primers for PCR
 * Consulted with David Ball about fluorescent proteins; contacted other iGEM team members about the filters they have available for fluorescent proteins; made decision on which fluorescent proteins to use for the biosensor
 * Read and discussed articles on USER and IM-Fusion; decided on the USER standard of fusion
 * Outlined Initial Project Design Report
 * Constructed primer sequences to use for PCR for copper promoter


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