Matt Gethers/CRI, Thailand/Labwork/Electroporation

=Electroporation Protocol=


 * 1) Grow up a 6 ml overnight culture of Pseudomonas in LB.
 * 2) Spin down the culture at 5,000 RPM for 5 minutes at room temperature.
 * 3) Wash twice in 1 ml 300 mM sucrose.
 * 4) Resuspend pellet in 100 &mu;l 300 mM sucrose.
 * 5) Add the 100 &mu;l to a cuvette (also at room temperature) and pulse at 2.5 kV, 200 &Omega;, and 20 &mu;F.
 * 6) Add 1 ml SOC to the cuvette immediately following the pulse.
 * 7) Spread 100 &mu;l on a plate containing the appropriate antibiotic and incubate at 37 degrees over night.