Klapperich Lab:Streaking bacteria on Agar plate

Overview
This protocol describes the procedure for streaking bacteria on an Agar plate.

Materials

 * Agar plate(s)
 * Bacteria sample from old plate
 * Inoculation loop
 * Bunsen Burner
 * 70% Ethanol
 * Parafilm

Procedure

 * 1) Wipe down the bench with 70% EtOH and start a Bunsen burner flame.
 * 2) Label new agar plate(s), on the bottom, with your initials, the date and strain of bacteria, and prepare plastic beaker for containing the disposable at the side.
 * 3) Flame until red to disinfect the inoculation loop, from top toward the loop, and allow it to cool down by inserting to loop into the agar at a corner of the new agar plate.
 * 4) (work by the flame) Open the old streaking plate, wipe down water vapor on the cover with Kimwipes, and take one (1) pure culture with the loop.
 * 5) Slide the loop over the new agar plate. First start circulating ~10 times at a corner of the streaking area, followed by moving the loop in zigzag patterns to spread the colony on all 4 quadrants over the plate. Between each spread, flame the loop to disinfect and allow it to cool down. Cross over the previous quadrant 2~3 times and avoid cross over the previous quadrant more than once or into any other quadrants for the last streak.
 * 6) Cover the new dish and seal the edge of the old plate with parafilm.
 * 7) Incubate the new plate @37C in a upside-down position overnight (~24hrs)
 * 8) Bleach and wipe down the bench with 70% EtOH again at the end.
 * 9) Take the plate(s) out from the incubator on the next day and store it inside the fridge with parafilm sealing the edge.
 * 10) Bleach the old dish (entirely cover the plate) and seal with parafilm. Dispose it in the biohazard trash.

Contact
Sylvanus Lee