IGEM:PennState/Labbook/NoahJohnson/2007-8-21

August 21, 2007


 * Set up 20uL double digests with psB1A2 as well as sequential digests with EcoR1 and SpeI
 * Used MP from galen's box marked:
 * 6B for E+S
 * 4B for E
 * 3B for S
 * Started incubating at 11:03am


 * Ran gel on restriction products
 * Started @ 1:50pm
 * Started an overnight ligation at 16C with old crp* 4uL restriction E+S from yesterday and the gel extracted psB1A2 restriction E+S from 8/10
 * Used a 6:2 ins:vect ratio

9:30-6:30