NTA surface preparation

Overview
The purpose of this protocol is to provide a detailed method for the preparation of an NTA chip for the Biacore S51.

Materials

 * NTA Sensor chip with an un-used Flow Cell
 * Reagent Rack 1
 * Small Plastic vials (4), type BR-1002-12
 * 10mM Glycine pH 2.2 (150ul)
 * 50mM NaOH (150ul)
 * BiaNormalization Solution (200ul)
 * 52ug/ml MgCl2 (30 ul)

Procedure

 * 1) Put at least 150ul of 10mM Glycine pH 2.2 in to a small vial and place in the Biacore rack
 * 2) Put at least 150ul of 50mM NaOH into a small vial vial and place in the Biacore rack
 * 3) Create new or re-use a small vial of BiaNormalization Solution and place in into the rack at position F6
 * 4) Eject the rack currently in the Biacore and replace with the rack containing Glycine and NaOH.
 * 5) Start and interactive run with a PBS-P(0.005%) mobile phase
 * 6) Flow rate = 30
 * 7) Flow Cell = The new Flow Cell
 * 8) Spots = 1 & 2
 * 9) Enter the Glycine and NaOH into the solutions table
 * 10) Mark the appropriate settings
 * 11) Prepare the flow system if necessary
 * 12) Normalize the detector
 * 13) Drag the samples to their appropriate locations
 * 14) Preform 3 alternating 1 minute injections (30 ul) of Glycine and NaOH
 * 15) Check the NTA surface by starting another interactive run and inject 52ug/ml MgCl2 for 1 minute at a flow rate of 10 ul/min.

Contact
Talk to Jason Fuller to discuss this protocol.