User:Karmella Haynes/Notebook/Polycomb project/2010/01/13

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

01/13/10

 * &#x2713; Polycomb-ATF lines: expand selected clones in 6-well dishes (1 clone/ well)
 * &#x2713; Protein preps (for Western): from Dox+, selected Polycomb-ATF clones

Protein preps > Polycomb-ATF clones > KAH126-129, 132, 133 Dox+ from 24-well plates --> Let KAH130, 131 grow for > 2 days before prepping (low cell density) ( Prepped on Saturday 1/16 ) > Use 100 μL RIPA + protease inhibitor/ sample --> Incubate @ 4°C, rotating, overnight


 * }