SBB09Ntbk-SamNg

Protocols
EspP_Autotransporter Myc_Tag

*Sam Ng 9 March 2009:
Completed Protocols: Myc_Tag - Analytical Digest Myc_Tag - Sequencing

Additional Notes: N/A

*Sam Ng 6 March 2009:
Completed Protocols: EspP Autotransporter - Sequencing Myc_Tag - Picking of colonies Myc_Tag - Miniprep purification of DNA

Additional Notes: N/A

For Monday Proceed With: - Myc_Tag - Restriction Digest Check

*Sam Ng 4 March 2009:
Completed Protocols: EspP Autotransporter - Analytical Digest Myc_Tag - LR Gateway Transfer Myc_Tag - Transformation by Heat Shock

Additional Notes: N/A

For Monday Proceed With: - EspP Autotransporter - Sequencing

*Sam Ng 27 February 2009:
Completed Protocols: EspP Autotransporter - Picking of colonies EspP Autotransporter - Miniprep purification of DNA

Additional Notes: Went into Restriction Digestion Check, but incorrectly used all 5 ul, immediately stopped reaction and proceeded with Zymo Cleanup.

For Wednesday Proceed With: - EspP Autotransporter - Restriction Digest Check (8 ul)

*Sam Ng 27 February 2009:
Completed Protocols: EspP Autotransporter - Ligation of EcoRI/BamHI digests EspP Autotransporter - Transformation by heat-shock

Additional Notes: N/A

For Monday Proceed With: - EspP Autotransporter - Picking of colonies

*Sam Ng 25 February 2009:
Completed Protocols: EspP Autotransporter - EcoRI/BamHI Digest of PCR Products EspP Autotransporter - Regular Zymo Cleanup (2)

Additional Notes: Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**

For Friday Proceed With: - EspP Autotransporter - Ligation of EcoRI/BamHI digests

*Sam Ng 23 February 2009:
Completed Protocols: EspP Autotransporter - Analytical Gel EspP Autotransporter - Regular Zymo Cleanup (1)

Additional Notes: Lane 3 (~1.5kb)

Regular Zymo Cleanup (33ul)

For Wednesday Proceed With: - EspP Autotransporter - EcoRI/BamHI Digest of PCR Products

*Sam Ng 18 February 2009:
Completed Protocols: EspP Autotransporter - Oligos EspP Autotransporter - Cloning by PCR

Additional Notes: Received oligos Osn0001-F and Osn0002-R: Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol

Ran PCR with EHEC For Monday Proceed With: - EspP Autotransporter - Analytical Gel - EspP Autotransporter - Regular Zymo Cleanup (1)