Matt Gethers/CRI, Thailand/Labwork/HmgR Binding Assay/Week of 7.13.08

=7.15.08=

To Do:
 * Come up with shortlist of DNA fragments to use in gel shift assay.
 * Look for HmgR and GpxR boxes (putative boxes from Mayuree) at the HmgA/HmgR promoter (they're divergently transcribed, remember).
 * Maybe write a script to help me find the boxes.

Mayuree suggested a probe, but wants to look into using two different dyes. Want to avoid quenching or FRET - look at excitation and emission spectra.

sources: http://www.iss.com/resources/spectra.html

http://www.bdbiosciences.com/spectra/

https://www.omegafilters.com/curvo2/index.php?dyes=269