IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/07/Zhangs

Identify the RBS+CI+T ligation product by electrophoresis Result (from left to right) 2K+CI+1-23L①~⑤ (plasmid), 2M+CI+1-23L①~⑤ (plasmid), 1J+CI_1-23L①~⑤ (plasmid), RBS plasmid control, Marker The 1-2K+CI+T1, 2, 3, 5 & 1-2M+CI+T1, 2, 3, 4, 5 & 1-1J+CI+T1, 2, 4, 5 are target plasmids. Choose samples: [1-1H⑤,1-2G⑤,1-5N⑤, 1-2I⑤, 1-11N④, 1-2K⑤, 1-2M⑤, 1-1J④]+CI+1-23L, 8 samples in total. Double-digestion of RBS+CI+1-23L insert

Electrophoresis Result (from left to right) 1-2K, 1-2M, 1-1H, 1-11N, 1-2G, 1-2I, 1-5N, 1-1J, Plasmid Control, Marker 1-2K+CI+1-23L insert digesting failed

Gel Extraction for RBS+CI+1-23L Ligate the RBS+CI+1-23L insert and T7 promoter vector System