Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-13

--David Roche 10:55, 13 August 2009 (EDT) PCR monkey


 * James Field 13:32, 13 August 2009 (EDT):


 * Colanic acid assay 2.1 protocol - completed
 * Completed miniprep and confirmed that BioBricks taken out of registry were correct.


 * Charles Fracchia 13:41, 13 August 2009 (EDT):
 * Explained thoroughly assays and genetic circuits
 * Started modelling on LacI repressible promoter
 * Designed site directed mutagenesis primers for PAH serine 16 -> Glutamine
 * Sacrificed a sheep for good luck
 * Beefed up the description of PAH activity assay and added step 0 of sonication to the protocol
 * Helped loading Gel electrophoresis for biobrick checking


 * Kun Xue 15:25, 13 August 2009 (EDT):


 * Researched on cI protein sequences and how the Harvard cI sequence might be wrong
 * Beefed up description of Cellulase assay and output data

Royah Vaezi:
 * Midi-prep for PAH. Last steps to be completed tomorrow.
 * PCR of OtsA and Dam. Re-did OtsB PCR using mid-temp PCR product from James C's last round of PCR. Will run all PCR products gained on a gel tomorrow AM to check.
 * Restriction digest of B3023 with XbaI and SpeI (overnight). Vector will be digested tomorrow ready for ligation. Aim is to have the BioBrick on Tues...