IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/09/20

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dspB Track
Plate result: 9 colonies

Colony PCR
PCR Cycles:
 * 98C @ 3min
 * Cycle 27x:
 * 98C @ 10 sec
 * 72C @ 30 sec
 * 72C @ 40 sec
 * 72C @ 10 min
 * 10C @ hold

Start: 12:54pm End: 2:02pm

Phusion : VF2 - 64C; VR - 64C

Gel verification colony PCR products
Gel orientation: Results:
 * Protocol: gel verification protocol in Protocol (SOP)
 * Machine conditions: 0.5x TBE buffer, 100V, 45min

O/N culture:

 * Protocol: SOP
 * With 5mL of culture with chlor 1 (A,B,C,D,G,H) in 37C at 4:54pm

Transformation (for His) again

 * 9pm
 * no colonies from yesterday
 * changes:
 * 1) Use 7uL of ligation mix
 * 2) Use 400uL of SOC instead of LB Broth

PCR using Rafael's method Attempt #4

 * Gave to Rafael to finish.


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