User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/20

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04/20/10

 * &#x2713; Digests: check plasmids KAH156, 157/MV2; KAH96, 142, 146, 147/V0201

Digests --> NEB AvaI (buffer 4) for KAH156, 157/MV2 (AvaI cuts 1x at end of insert and 1x in fshPcCD of KAH157) --> Fermentas FD XbaI for KAH96, 142, 146, 147/V0201

--> 37°C/ ~1 hour

--> Unsure about KAH156, 157/MV2; re-submit for sequencing


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