User:Andrew Barney/Notebook/Old Fruit Fly Lab/2007/10/17

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Lab 2
10/17 -


 * Observation:

Tons of larvae in each reciprocal cross vial. Seems like there are about 3X as many in vial 2 (sepia females) than in the wild female vial.

Experimental Protocol
Vial 1 is Male Wild X Female Sepia Vial 2 is Male Sepia X Female Wild
 * 1. Make 2 new culture vials.
 * 2. Label one Male wild X Female sepia, and the other Female wild X Male sepia.
 * 3. Transfer 8 virgin wild females flies to vial 2. Transfer 8 virgin female sepia flies to vial 1.
 * 4.Anesthetize and sort out 8 male wild flies and 8 male sepia flies.
 * 5. Place the male wilds in vial 1. Place the male sepia in vial 2.
 * 6.Store in incubator at 75 Degrees °F.
 * 7.Record progress.
 * 8.When pupa form, release adults. (old adults)
 * 9. When more than 50 flies hatch, use flynap, and sort them.
 * 10. Find MOI (mode of inheritance)


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