Drummond:MisfoldingCost2010

This page supports "Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast," K. Geiler-Samerotte et al., Proceedings of the National Academy of Sciences 2010 (paper, Supporting Information, Appendix PDF/.docx).

Experimental Procedures
All procedures are described in the paper and, in detail, in the Appendix (PDF,.docx) and Supporting Information.

Supplementary Data
Data used to generate the figures in the paper (Excel format) including cell counts for fitness experiments, fluorescence and cell counts for YFPm3 titration experiment, KG079/KG071 protein ratios for induced and uninduced conditions (.xls, zipped, ~700k).

Protein database (FASTA format, zipped) for protein identification and quantification of strains KG071 (harboring YFPwt) and KG079 (harboring YFPm3) (text, zipped, ~2.4M).