SBB09 Construct2

PCR ca998/ca1363R on pBca9145-Bca9494      (1539 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5              (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-Bca1363    {Pbad.rbs.pp.ST}

ca998	Forward Sequencing of pSB1A2/pSB1A3 gtatcacgaggcagaatttcag ca1363R	Reverse StrepTag ccataGGATCCctgcccagactgCCCTCCCTC