User:Tara K. Luckau/Notebook/Team ConGen/2011/07/01

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Diversity Panel Analysis

 * each sample was replicated 4 times ... shown is a representative from the replicates
 * Impressions:
 * ladder looks great!
 * some things worked
 * a bit of pull-up/bleed-through
 * variability between samples - yay!
 * Preliminary Scores:
 * [[Image:20110701 FragScores.jpg|300 px]]
 * HOL and LOM did not amplify
 * TP3 had an extraneous peak in the green lane - could possibly be Scun3

Water

 * [[Image:20110701 FragH2O.jpg|500 px]]

RAJ

 * [[Image:20110701 FragRAJ.jpg|500 px]]

HOL

 * [[Image:20110701 FragHOL.jpg|500 px]]

SYR

 * [[Image:20110701 FragSYR.jpg|500 px]]

CPN

 * [[Image:20110701 FragCPN.jpg|500 px]]

TP1

 * [[Image:20110701 FragTP1.jpg|500 px]]

TP3

 * [[Image:20110701 FragTP3.jpg|500 px]]

LOM

 * [[Image:20110701 FragLOM.jpg|500 px]]

Lane Overlays

 * shows how all samples clump together
 * red circles indicate marker locations
 * blue lane bleeds through to green lane
 * [[Image:20110701 FragBlue.jpg|900 px]]
 * [[Image:20110701 FragGreen.jpg|900 px]]


 * zoom in on a particularly pretty sample (SCOC SYR2 X01 b)
 * trying to identify the markers:
 * going by color, the three markers that worked in this multiplex should be Scun2, Scun10 and Scun11
 * going by size, as compared to the gel from 23 June 2011, the three markers that worked in this multiplex should be Scun3, Scun11 and Scun15 (if the dyes got shifted)
 * [[Image:20110701 FragSCOCMP1_3markers.jpg|700 px]]
 * here's the map from 14 June 2011:
 * [[Image:20110614 SCOCMP1Map.jpg|1100 px]]


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