IGEM:Peking/2007/Switch-Notebook/2007-8-23

plasmid mini-prep
T1TE-PMD18T T1T2-pcc010 lacI-pcc009

colony PCR
test pcc009-lacI, no one is right!



colony PCR
test RS1, RS2, RD2, SD1, CD1 with GFP-plx007



some lanes seem to be right, just cultivate them, miniprep and double digestion(XhoI, SalI) tomorrow!