7-AAD

7-aminoactinomycin-D

Purpose

 * Staining dead cells

Procurement

 * Most standard companies
 * BD Biosciences
 * eBioscience

Use

 * 546/647
 * Nucleic acid dye
 * Often used in conjunction with Annexin-V when assaying for apoptotic cells
 * "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Safety

 * No significant safety considerations (BD Biosciences MSDS Sheet)