Joyce: Extraction of DNA from an agarose gel

Procedure

 * 1) Remove bands from gel on the preparative setting of a UV illuminator
 * 2) Place in an ependorff
 * 3) Mash up bands
 * 4) Add an equal volume of phenol
 * 5) Vortex for 2 minutes
 * 6) Place at -70°C for 30 minutes
 * 7) Thaw for 10 minutes at 37°C
 * 8) Repeat steps 4-7
 * 9) Spin at max speed for 1 minute
 * 10) Add 20 ul of 3M NaOAc (pH 5.2)
 * 11) Spin at full speed for 5 minutes
 * 12) Pipette off aqueous phase into a new ependorff
 * 13) Adjust salt concentration to 0.3 M
 * 14) Extract with 1x phenol, 2x ether, and precipitate