User:Robwarden/Notebook/Divalent Ligand Validation/2009/03/20

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 * style="background-color: #EEE"|[[Image:TBL.PNG|300px]] Divalent Ligand Validation
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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EcoRI Digest
10 μL DNA 2 μL 10X Buffer EcoRI -> 26 μL 1 μL EcoRI           -> 13 μL 7 μL MilliQ          -> 91 μL

Incubate at 37°C for 1hr.

Results

 * No correct colonies
 * Will redo cloning strategy next week


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