IGEM:IMPERIAL/2007/DNA Constructs

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Constructs Overview
Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details.

A new page is currently being developed!

Cloning Table
Status of biobricks cloned and their applications:

Ligation Table
Status of parts ligated and their applications:

Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Maxi - maxiprepped; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3); status - stocks running low]

Parts from the Registry
Physical information of biobricks:

Our Parts
Physical information of our ligated parts:

Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced) Concentrations are given in ng/μl

Part Graveyard
General information on parts we do not like...

Construct Diagrams
The original (old) diagrams page was moved to old construct diagrams page. This page also contains the obsolete parts.

CBD Constructs
ptet GFP
 * Availability: Found in registry as BBa_I13522

pT7 GFP
 * Availability: Found in resigry as BBa_E7104

ptet DsRed express
 * Availability: Requires ligation
 * Promoter can be found in registry as: BBa_R0040
 * DsRed express is to be ordered from GeneArt

pT7 DsRed express
 * Availability: Requires ligation
 * Promoter is being PCRed from plasmid derived externally
 * DsRed express is to be ordered from GeneArt

ID Constructs
pLux GFP with LuxR
 * Availability: Found in registry as BBa_T9002

pLux GFP with LuxR
 * Availability: Found in registry as BBa_J37032

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