User:Anthony Salvagno/Notebook/Research/2009/02/24/gDNA digestion try 2

I remembered doing this stuff, but forgot to write it up since it was such a short day at Osley Lab. Anyways. Here is what I did in quick:


 * 1) prepared:
 * 2) *24ul gDNA
 * 3) *7.5ul NEB Buffer 2
 * 4) *7.5ul 10x BSA
 * 5) *4uL XhoI
 * 6) *32uL H2O
 * 7) total of 75uL
 * 8) after 2 hours I added 3uL more XhoI
 * 9) waited another hour or something
 * 10) EtOH extraction
 * 11) This cocktail gave us 42.6ng/uL gDNA after digestion

I also have (in my notebook 1.0) 22ng/uL of pRS DNA. That is probably from the gel extraction.