Registry/Measurement kit/Notebook/2007-6-16


 * Plates with transformation of ligation product seem to be overgrown.
 * Streaking them out on new Tet plates to get single colonies.
 * Actually, the problem is most likely that the tetracycline plates didn't work.
 * Poured new Tet and Chloramphenicol plates
 * Tet: (1 in 1000): added 500µL to 500mL
 * Chlor: (1 in 250): added 2.5 mL to 500mL
 * Transferring colonies from old plates to new ones using Jason's makeshift protocol:
 * 1) Add 1 mL of LB to the plate
 * 2) Get the LB to cover all of the colonies
 * 3) Pipet, or if you're feeling brave, pour the LB into a tube
 * 4) Plate 50~100µL onto the new plate


 * Placed excess of transfer LB in the -4 fridge.