IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/15

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Entry title

 * Transformed J23119(C)(S/P)+S+I7 and J23119(C)(S/P)+S+I2
 * Gradient PCR set up for K145151(x2) (1 with primers 3 and 4; 1 with primers 9 and 10)
 * Gel run for this PCR
 * Gel order:
 * Row1: K145151(3,4)(1 to 12); blank; ladder
 * Row2: K145151(9,10)(1 to 12); blank; ladder
 * S03879 Gradient PCR (12 values)
 * PCR of I13502; E0420; I13507; E0430; I13504; S03879 with 1 and 2 primers
 * PCR of K145151; K156011; K143065 with B0034 Primers
 * Gel run order
 * Row1: Ladder; S03879(G1 through 12); S03879(1,2)
 * Row2: Ladder; I13502; E0420; I13507; E0430; I13504; Blank; K145151; K156011; K143065; Blank; J13002(1)(S/P); J13002(2)(S/P)


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