IGEM:UC Berkeley/2006/Conjugation

Rlambda (marker swap Procedure) TlambdaOlambda

protocol for conjugation
donor protocol Recipient protocol
 * 1) start out with saturated 1 mL of culture
 * 2) spin down (top speed for 10 sec.), discard fluid
 * 3) resuspend in 1 mL LB
 * 4) vortex, spin, discard fluid
 * 5) resuspend in 1 mL LB, vortex
 * 6) put 200uL of culture into 2 mL of LB (if arabinose is needed put in 100 mg/mL)
 * 7) grow 2 hours
 * 8) do Assay
 * 1) take aliquot and thaw
 * 2) dilute 10 fold into LB (ie. put 200 uL into 2 mL of LB)
 * 3) grow 2 hours
 * 4) do Assay