User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/01/Taxol Crystals Can Masquerade as Stabilized Microtubules

Paper
Taxol Crystals Can Masquerade as Stabilized Microtubules

Review
Great paper describing how tubulin will form on crystalline structures of Taxol. This is great. This describes how I was able to get this structure from my data. It makes complete sense. Microtubules are floppy when in solution. This structure is totally rigid which could mean that I have tubulin forming on a Taxol crystal.

   

I would like to note what this group calls BRB80. This is different from what I use but oh well.
 * 80 mM PIPES
 * 1 mM EGTA
 * 2.1 mM MgCl2
 * 1 mM GTP
 * 4% Glycerol



Questions
So if Taxol makes these crystal structures, what happens when Taxol is in D20? Does it crystallize? Could this be where I'm getting the "log" structures in my data?

Finally, are the ribbon structures that form when microtubules are polymerized in heavy water be from the crystallization of Taxol?



PLOS One structure
So the basic structure of this paper is,
 * Intro & statement of purpose.
 * Results and justification of purpose.
 * Materials and methods.