Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgA Downstream Fragment in pUC18/Calculation

=Length of region including HmgA downstream fragment and region between SmaI and M13 for primer=

Length of HmgA downstream fragment (after cutting with NcoI): 1021 bp

Distance between SmaI (434) and HindIII (399): 35

Distance between HindIII and M13 primer binding site: 47

Total: 1103