NanoBio: Supplemented M9 Media

=M9 media=

This recipe makes 1L of media supplemented with
 * 0.4% of selected carbon source
 * Examples of carbon source: glucose, lactate, glycerol, succinate, acetate, fumarate
 * 1 mM thiamine hydrochloride
 * 0.2% casamino acids

Combine all solutions using sterile technique:
 * 200 mL 5x M9 salts
 * 30 mL of 10mg/mL thaimine hydrochlorite
 * Dissolve 10mg per mL of H2O
 * Filter-sterilize using a 0.22μm filter
 * Light-sensitive. Store covered in foil
 * 20 mL of 20% carbon source
 * 20 mL of 10% casamino acids
 * 20 mL of 0.1 M MgSO4
 * 1 mL of 0.1 M CaCl2
 * deionized water to 1L (709.8mL)

Store at 4°C.

Note: Adding Fe increases cell density. For example, FeSO4·7H2O at 0.01 mM


 * Heather M. Jensen 19:52, 11 June 2008 (CST):

Return to Protocols.