IGEM:metu/2009/Notebook/wound dressing/2009/09/15

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15.09.2009
1. Double digest(fast digest) of granulysin, KGF, Lard 1 and Backbone

Plasmid 4th was monodigested with XbaI.

Double digest of Poxygen with XbaI and SpeI

For KGF; 5 microliter DNA 2 microliter SpeI 0.5 microliter EcorI 2.5 microliter Buffer R 15 microliter water nuclease free

For Granulysin; 15 microliters DNA 3 microliter Buffer O 1.5 microliter PstI 1.5 microliter EcorI 9 microliter water total: 30 microliter

For Lard1; 15 microliters DNA 3 microliter Buffer O 1.5 microliter PstI 1.5 microliter EcorI 9 microliter water

For BB; 10 microliter DNA 1 microliter EcorI 1 microliter PstI 2 microliter Buffer O 6 microliter water nuclease free