IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-9-25

Cyano continues! And you all thought we were dead...

To do

 * Chiba letter
 * Miniprep Nick's plates and sequence
 * Begin taking aliquots of M2-7 J04500+KaiC at time points
 * The other (less than 6) stage1 ligations

Verification of J04500+KaiC
Based on the (20!) sequencing results from way back in August (how is it freezing now...)I:


 * Created a file on MCB1001 (small room, left computer), ZS_J04500+kaiC which contains the expected plasmid we want
 * Aligned all 10 VF2's to the expected file
 * Tested fully one of them, M2-7

And we have the correct plasmid! First biobrick...