Berglund:Transcription Capped

In vitro Transcription with ultrahot/capped RNA for splicing 5µl	     5xT7 buffer 1µl	     400mM DTT 2.5µl	     rNTP Mix (5mM A, U, 1mM G) 2.5µl	     0.1mM CTP 5µl	     P32 CTP 1µl	     10mM M7cap 0.5µl	      Rnasin 0µl	      water 5µl	      plasmid or PCR (1µg) 2µl	      T7 polymerase 2.5µl	      10xyeast pyrophosphatase 25µl        total

1x buffer: 40mM Tris pH 8 6mM MgCl2 2mM spermidine 10mM NaCl 25mM Tris pH 9