IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/09

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8/9/10
10:00A AM

PCR verification AGAIN!!

Per reaction: 19 μL mastermix, 1 μL template

Progam:

Samples:   B0034ArsRSDM1B0015pSB1C3 1  B0034ArsRSDM1B0015pSB1C3 2  B0034ArsRSDM1B0015pSB1C3 3 (1')  B0034ArsRSDM1B0015pSB1C3 4 (2')  LamBF1R 1'  LamBF1R 2'  LamBF2R</li>  LamBF2R Green</li>  pArsR F1R2 1'</li>  pArsRF1R2 2'</li>  pArsRF1R2 1</li>  pArsRF1R2 2</li>  Negative control (no primer) template B0034ArsRSDM1B0015pSB1C3 1 </li>  Negative control (no template)</li> </ol>

Primers: VF2, VR 10 micromolar

1:45 PM

Gel

Top

Bottom:



2:45 PM

made sequencing reactions:

1.5 μL ArsRSDM2

2 μL 10 micromolar VF2

6.5 μL H2O

Made 2 reactions in case sample needed to be re-run.

3:35 PM

Recieved and suspended new primers to 100 micromolar. Then diluted aliquots to 10 micromolar.


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