Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/20

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20 October 2009 Lab Meeting
‡ Announcements ‡ Flu R01:Integration † Sample Concentration (Lead: Jane, Team: Jaephil)
 * Attending:
 * Missing:
 * Presentation:Dr. Tushar Bansal, post doc applicant. 2pm, please BE ON TIME.
 * Oakridge deadline is 1 Feb 2010.
 * PAPERS Drafted before MicroTAS
 * Posters for MicroTAS
 * New design - address evaporation loss, Reagent delivery and storage solution. Consider marginating flows?
 * MDCK Cells are not propagating. Try troubleshooting efforts. Sonali will do on Monday.
 * Cassidy needs to see plaque assay again - so when cells are up, she needs training.
 * Up to high 10^7 for positive control. Silver substrate no signal.
 * Jane working on the cell lysate control.
 * Main loss is at the outlet/sample collection: tangential filtration design in drawing stage.
 * Contacted EC Shaw about rubber stamp mold for new design for evaporation.
 * Set up COMSOL and StarCD, look for governing equations for simulation of evaporation.
 * Committee meeting setup

† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) † PCR - CMI (Lead: Qingqing) * test liquids to fill the empty channel(Mineral oil,Water) * Deep reservoir design - deformation during bonding - fabrication difficulty - Fluidic control difficulty * third generation design to solve these problem - the mold and the chip has been made, and will be tested this week. - ATCC DNA and Primer pairs for both toxin A and B has been ordered. - real-time PCR has been done on ABI, waiting for the result. † HDA (Lead: Jaephil, Team:Sonali) '''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc ) ‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing) ‡ Agilent Automated Sample Preparation (Lead: Alex) ‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)  ‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks) ‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA) ‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)
 * Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between.Send out masks by 10/21, functional chips to be delivered by 10/26. [Update] functional chips ready by 10/21-22, Experimental results of channels to be delivered on 10/27  [Hussam/Jessie]
 * STD recipe, STD silica beads, just A,B, and C.
 * controls for these: empties, non-silica monolith and full silica monolith, std. recipe.
 * Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, To be repeated with Alex and Mark 
 *  Look into modifying Alex's pneumatic setup for the PATH guys. Instead of building the straw array over here NOTE: Sean
 * PLanning net silica per channel experiments. with 700nm, 3x possible. Deliver around 11/5/09.
 * New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution. Use more diluted sample for future optimization experiments? Channels were 700nm silica/1X. pfu/ml is 10^6.
 * Tried 2 more chips with 3X 0.15um Silica. Chips are breaking b/c of no crosslinking. SPE was coming out even at 2ml/hr flow rate.
 * Running no silica and no SPE chips with virus. (JC/RNA)
 * QQ will work on the initial integration steps of SPE + RT (reservoir)+ PCR.
 * PCR of C.Difficile DNA
 * PCR2 Paper formatted for LOAC this week.
 * CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations.
 * Start planning R01 for Submission on 2/5/10. MM, JD, CMK.
 * JD will train QQ how to make HDA chips. This may entail moving from cutter plotter to embossing steps. JD and QQ to meet ASAP to plan the tech transfer.
 * Paper submitted 10/6.
 * Sonali to train Lisa on SPE.
 * QQ to run test PCR on chip with genomic DNA and Toxin B primers.
 * Meeting 9am Monday (every other week)
 * Paul finishes work on nozzles on Wednesday. Then first testrun and rewriting of software for "HotDog"-Run on Machine.
 * First Draft of Paper (Lysis of Yeast Cells) ready. Work ongoing on "Conclusion"-Section of Paper.
 * Metal piece modification for the integration - Parts distored and is begin fixed.
 * paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli.
 * New experiments happening now.
 * Cathie will submit paper inquiry.
 * IRB approved.
 * Cathie submitted the companion BU IRB form for exemption. Revised, still still waiting.
 * Phone meeting with PATH this week. 10/13.
 * UGs purchasing parts for straw machine for 709.
 * UGs purchasing reagents for running extraction experiments and doing PCR.


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