User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/02

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] qPCR GAPDH, Silverstaining
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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GAPDH
As internal control also GAPDH need to be measured,
 * 20 uL reaction volume, as given by the machine
 * Human GAPDH primer (Applied biosystems)
 * Taqman Gene expression master mix

Silver staining

 * Repeat of yesterday on same gel, fresh 0.1% Silver nitrate was prepared

Results

 * After doing the Coomassie staining a smear could be vaguely distuingished around 250 kDa
 * But during the meeting today results were presented showing that the Enspire 2300 has a malfunction leading to wrong protein concentrations
 * qPCR results for AKAP12

Discussion

 * ✅ Ow..

Attachment
   [[Media:02072010_qPCR_GAPDH.xls|qPCR for GAPDH]]


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