Shreffler:Notebook/Staff Meetings/2010/05/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] ShreffLab Staff Meetings
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Agenda Items
Alex's work
 * JAX
 * JAX data getting organized
 * Alex may take a stab at editing R script
 * Alex will try to set up scanner
 * PM
 * Alex will update PM_baso data
 * received PM -- will review what to do with it
 * how is it resuspended for tissue culture -- Wayne will ask EM
 * R03
 * 2/4 experiments good
 * repeat next week with 1X DHR
 * Carbo
 * alex will set up new compensation controls
 * alex will create a FJ analysis template file
 * some recent samples have shown response to carbo as per AM

Bert's work


 * Ben Medoff's PCR gives better results -- dramatically lower Ct values across the board
 * Despite this, CPE response looks weak -- one new lot of StemSpan versus old
 * somewhat stronger in X-vivo (n=1)
 * ? of whether only CPE response or other pathways are also suppressed
 * perhaps we need fresh CPE?
 * some hope in a poor quality experiment that ALDH inducing activity is fractionating
 * WS will contact Luda about getting a little aliquot

Action Items

 * Wayne will talk to Ben about using qPCR more regularly
 * Bert will compare LPS response
 * run fraction screening experiment on Ben's machine
 * repeat fraction screening experiment with pooled fractions
 * get larger column for bigger run
 * contact Steve about using HPLC