User:Anthony Salvagno/Notebook/Research/2009/01/26/Restriction Endonucleases

Wikipedia
I tend to go here for the most basic information and since it always seems like a good starting point. After I read about them there I will check out the link Koch provided me (next section).

Nothing too complicated here. Rest. Enzymes, cut the DNA at a specific location. The site can either be palindromic (where the complimentary strand is the inverse of the other) or not. You can also have sticky ends or blunt ends. The sticky end allows for a complimentary strand to be attached (via ligation) to the site. I don't know what you can do with blunt ends.

Wikipedia goes into the types of RE's and from the looks of it Type II one's are the most used. Unfortunately the naming of these things are not the same as the type (ie EcoRI is not a type I RE).

Restriction Enzymes Overview
I don't like this page... the font hurts my eyes.

Interesting note:
 * Type I enzymes are complex, multisubunit, combination restriction-and-modification enzymes that cut DNA at random far from their recognition sequences. Originally thought to be rare, we now know from the analysis of sequenced genomes that they are common. Type I enzymes are of considerable biochemical interest but they have little practical value since they do not produce discrete restriction fragments or distinct gel-banding patterns.

Maybe we can use these for SDM since they may provide random fragments of unknown size. It seems the cut locations are also random and so the locations too would not be known. Another sort of proof-of-principle.

Some words to learn:
 * homodimers
 * heterodimers
 * monomers

(Test)