User:Daniel Goodman/Notebook/Cluzel/2010/04/05

PZE-12-CFP vector from JM Kim

 * Vector Nomenclature: http://www.expressys.com/main_vectors.html

Electroporations
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.

Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book
 * used straight glycerol instead of GYT
 * used SOC medium (1 ml per reaction)
 * 1 ul of DNA per experiment (approx 52 ng for my plasmid, did not nanodrop Jeff's spec plasmid)
 * 1.8 kV, 1 second shock

Put in 37 degree shaker w/ the SOC for 1 hour. Spun down, supernatant removed to ~50ul each, resuspended, plated and spun onto correct antibiotic plates w/ LB. Put in 37 degree incubator overnight at 7:45 pm. Will take out tomorrow morning.


 * }