IGEM:IMPERIAL/2007/Notebook/2007-8-8

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/15 view=threemonths format=%name/%year-%month-%day weekstart=7

Transformation of Biobricks

 * 1) 15 biobricks were transformed into XL1-Blue
 * 2) Cells were grown for 1 hour and plated
 * 3) Plates were at 37°C overnight


 * 1. BBa_E0040 [GFP]
 * 2. BBa_I13352 [ptet-GFP]
 * 3. BBa_J5528 [pBad-GFP]
 * 4. BBa_J24677 [ptet-LuxR-plux-GFP]
 * 5. BBa_R0040 [ptet]
 * 6. BBa_I0500 [pBad]
 * 7. BBa_F2620 [ptet-LuxR-plux]
 * 8. BBa_F1610 [luxI]
 * 9. BBa_R0010 [lacI]
 * 10.BBa_E7010 [RFP]
 * 11.BBa_E7104 [T7 GFP]
 * 12.BBa_S03550 [araC]
 * 13.BBa_B0015 [Terminator]
 * 14.BBa_R0051 [pCI]
 * 15.BBa_S07037 [pCI-GFP]

Protocols can be found at Electroporation in the general protocols page


 * Plate 3 had no colony (possibly due to large plasmid size - difficult to transform)

Preparation of Competent Cells
Protocols can be found at Preparation of Competent Cells in the general protocols page
 * 1) BL21(DE3)-Gold was made electrocompetent