User:Amirah

Initial ideas

 * Build a self assembling and degradable scaffold
 * Self assembly would get rid of the use of extraneous material to form proper bonds.
 * If the material is degrabale, it can degrade leaving the scaffold behind.
 * Find coating to cover degradable scaffold that can select for viruses or cells that excrete scaffold material (collagen, calcuim bicarbonate etc.)
 * Find coating to bind proteins needed to assemble outer scaffold layer.
 * Coating must not alter assembly or binding of scaffold
 * Coating must be very selective for protein or cells needed to form outer non-degradable layer of scaffolding.

Experimental Implementation ideas

 * Use DNA as scaffold.
 * DNA is degradable
 * DNA has the ability to be self assembled into 2D structures (Rothemund), and 3D structures (Zhang)
 * Build 3D structures from adaptaion of 'raster fill method' (Rothemund)
 * Try the assembly of bone structures
 * Use bone morphogenic proteins, BMPs (Hosseinkhani)
 * Use mesenchymal stem cells, MSC (Hoseeinkhani)
 * Improved cell/protein seeding method onto DNA scaffold
 * RGD utilized to increase cell adhesion to 3-D scaffolds
 * RGD-modified poly(L-lactic acid)
 * Use oscillatory flow perfusion instead of static perfusion
 * Electrostatic coating of DNA bipolymer (Green)

Papers

 * DNA Folding
 * Rothemund P.W.K. 2006. Folding DNA to create nanoscale shapes and patterns. Nature. 440:297-302
 * Zhang Y., Seeman, N.C. 1994. The construction of a DNA truncated octahedron. J. Am. Chem. Soc. 116: 1661-1669
 * Mirkin C. A. 2000. Programming the assembly of two- and three-dimensional architectures with DNA and nanoscale inorganic building blocks.Inorg. Chem. 11: 2258-2272
 * Exploration of 3-D scaffold usages and seeding abilities
 * Hosseinkhani H., Yamamoto M., Inatsugu Y., Hiraoka Y., Inoue S., Shimokawa H., Tabata Y. 2006. Enhanced ectopic bone formation using a combination of plasmid DNA impregnation into 3-D scaffold and bioreactor perfusion culture. Biomaterials. 27: 1387-1398
 * Francoli S. E, Martin I., Sie C. P., Hagg R., Tommasini R., Candrian C., Heberer M., Barbero A. 2007. Growth FActors for Clinical-Scale Expansion of Human Articular Chondrocytes: Relevance for Automated Bioreactor Systems. 13: tentative
 * Alvarez-Barreto, J. F., Sikavitsas, V. I. 2007. Improved Mesenchymal Stem Cell Seeding on RGD-Modified Poly(L-lactic acid) Scaffolds using Flow Perfusion. Macromol. Biosci. 5: 579-588.
 * DNA nanoparticle seeding technology
 * Green, J. J., Chiu E., Leshchiner, E. S., Shi J., Langer R., Anderson D. G.2007. Electrostatic ligand coatings of nanoparticles enable ligand-specific gene delivery to human primary cells.Nano Lett. 4: 874-879.
 * Green, J. J., Chiu E., Leshchiner, E. S., Shi J., Langer R., Anderson D. G.2007. Electrostatic ligand coatings of nanoparticles enable ligand-specific gene delivery to human primary cells.Nano Lett. 4: 874-879.