User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/09

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Transformation Check Backbones
As expected the chloramphenicol plate was doggy, the other plates grew fine
 * pSB1A3 - ok
 * pSB1C3 - no growth
 * pSB1K3 - ok
 * pSB1T3 - ok

Overnight Growth Ligation
The ligation grew fine but this is expected to not have worked so part J04550 remains - colour is red/pink

Miniprep Ligation
I extracted the plasmid of the proposed ligation growth above

Overnight Growth strains
All strains grew:
 * Mach-1
 * DH5-alpha
 * JMV-101.

Competent Cells
I prepared 3 strains of competent cells:
 * Mach-1
 * DH5-alpha
 * JMV-101.

Check Competent Cells
I set up 3 transformations with J04450, Plate 1, A1, Spring 2010.
 * Mach-1: Carbenicillin
 * DH5-alpha: Carbenicillin
 * JMV-101: Carbenicillin

Transformation pSB1C3
I set up a new transformation of this part as the previous chloramphenicol plate was doggy

Overnight Growth BioBrick Backbones
10ml LB-Broth with respective antibiotics
 * pSB1A3 - Carbenicillin
 * pSB1K3 - Kanamycin
 * pSB1T3 - Tetracycline


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