IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/07/01

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Jason G

 * 2 streaked plates (from june 30th) taken out from 37C box at 8:35am
 * Staph grew on both plates without any signs of contamination.
 * Indicates that glycerol stock of staph made from original liquid culture (June 30th) can be used for OD testing.
 * Staph on streaked plates can be used.

2 for staph competent cell growth. Time inserted: 4:00pm To be taken out at 10:00am (10:00 am on July 02)
 * Grew ON culture (aerated, 32.5C, 180 rpm) of staph from streaked plates and glycerol stocks: 2 for OD measurement,

Gel verification of colony PCR products (from last day)
See protocol: Gel verification protocol in iGEM training manual Gel box #1: Lagally Box: 32 wells --> 8T1-8T20, 11T1-11T9, two 100bp ladders, W1 Gel box #2: Jefferies Lab Box: 20 wells --> 11T10-11T20, 6T1-6T8, 100bp ladder Gel box #3: AMBL Box: 8 wells --> 6T9-6T15, 100bp ladder


 * Machine Conditions: 70V, 60mAmps @ 60 min, 0.5x TBE buffer
 * DNA: 10uL, loading dye:2 uL

Gel results:
 * Gel box #1:


 * Gel box #2:


 * Gel box #3:

Vicki Ma 21:21, 1 July 2010 (EDT)


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