User:Karmella Haynes/Notebook/Polycomb project/2011/04/11

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04/11/11

 * &#x2713; Luc assay: transfections from 4/09/11
 * &#x2713; RNA prep: remainder of transfected cells (Note: yield too low to make good cDNA; do RNA isolation on whole sample next time)

Luc assay
 * Resuspend cells in 1 mL growth medium (do not aspirate or add new med.)
 * Collect 500 μL from each sample
 * Use 3x 100 μL for Bright-Glo luc assays
 * Use 25 μL cells (diluted in 75 μL DPBS) for cell counts (200 ng transfection samples only)


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