User:Tk/Notebook/MF/2009/11/21

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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Cutting DNA with EcoRI and PstI


Cut 1 &mu;g DNA in 50 &mu;l in buffer 2, BSA, 1 &mu;l enzyme


 * L1
 * EcoRI digest
 * PstI digest
 * E & P digest


 * GF
 * EcoRI digest
 * PstI digest
 * E & P digest


 * PP
 * EcoRI digest
 * PstI digest
 * E & P digest


 * Ent
 * EcoRI digest
 * PstI digest
 * E & P digest

L1 cannot be cut with PstI (note the methylation of CAG and CTG sites, preventing cutting) Other strains appear to be only partially methylated at these sites.


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