User:Kristina M Holton/Ovarian Notebook 2007 7 17

Bioanalyzer: 4 Ovarian Tissue Samples Suggestion: maybe too dilute
 * Diluted 1:10
 * Used Nano chip -> gel less viscous and easier to practice with
 * No rRNA bands, but miRNA bands ???
 * Used Micro chip + my pipettes -> good results
 * Easier to dispense gel + samples without bubbles

RNA MiniPrep: SW620, SW480

Use cells frozen 9/10/07 from P21/P17

First Round: (1 plate in 1 column)
 * SW480: OD 650, 65ug
 * SW620: OD 220, 22ug

Second Round: (1 plate in 2 columns)
 * SW480: OD 1132, 135ug
 * SW620: OD 1602, 192ug

Split 1 plate into 2 columns