IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/06/09

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Eric F.

 * Recieved S. Aureus from the Davies lab on a streaked plate. Strain is currently unknown, will know soon (through Rafael)
 * Used the Day Culture protocol from common protocols
 * Picked two colonies into two 14mL test tubes each containing 5mL LB broth
 * Made one control containing just 5mL broth
 * Incubated all 3 tubes at 37 degrees C for 9 hours
 * Control remain clear, others were very cloudy
 * Now being stored on the bench in a rack

Competent Cell Transformation

 * Tested the competent cells (DH5α and Db3.1 - made by Philip and Kevin) made yesterday using plasmid from last year labeled ASEM24(B) with Ampicilin resistance
 * Tested one from each centrifuge tube, plated labeled B1, B2, B3, α1 ,α2
 * Used the transformation protocol from common protocols
 * In step 1, the competent cells were allowed to thaw for 1 hour on ice, and were occasionally hand warmed
 * During step 2, noticed the α2 aliquot had too much volume, so we put it back in the -80 degree C and got a different one
 * Note: This aliquot was hand heated more than the others and was only thawed on ice for 10 minutes
 * 5 plates of transformed cells were spread onto Ampicilin plates and are incubating overnight in the biosafety room incubator at 37 degrees C
 * Need to be removed by 1pm tomorrow
 * Note: Need to plate more Amp plates tomorrow
 * Note: There was no control, if there is no transformation, we should test the DNA with last year's competent cells

PCR Previously Transformed Cells

 * Obtained results from the PCR of the transformed cells
 * Results were inconclusive and confusing, the ladders showed no movement, only the dye moved (the dye moves independently right?)
 * Picture is in the QS Track labbook
 * Had trouble getting a clear picture

Eric F.

 * Removed culture of BW27783 from the incubator (30 degree) at 10am
 * Control remained clear, tube containing E. Coli was quite cloudy
 * Need to send to Tec-Monterrey, tubes are on the bench in a rack