User:Anthony Salvagno/Notebook/Research/2009/11/12/Alpha DNA Oligos

I need a category for this page, but can't think of how to label it. Should I use the Ant Sequences Category?

Order Arrived
It is here! Hooray! I will document the information of each oligo below so that anyone can find it and use it in the future. For now I will literally transcribe it by hand, but a .pdf file would be useful too. Other suggestions? (Steve Koch 00:06, 13 November 2009 (EST):transcribing by hand probably easiest way to get pertinent information in...but scanning to PDF and uploading / emailing to kochlab notes is aslo probably good.)

Here is the page I started from. I will do all the resuspensions tomorrow pending Koch's approval.

Top BstXI/SapI
This is for ligating DNA with a specific BstXI overhang to DNA with a specific SapI overhang. We actually have this, but stuff hasn't been working so we ordered new stuff. Info: So I need to resuspend in 203.54ul of 0.1x TE. So weight wise, I will have 257ug in 203ul which is 1.27ug/ul.
 * picomole: 20354
 * micrograms: 257

Bottom Biotin
This is to complement the top bstxi/sapi oligo above. It has a nick and a biotin so that we can unzip with streptavidin microspheres. Info: So I need to resuspend in 308.17ul of 0.1x TE. So weight wise, I will have 320ug in 308ul which is 1.04ug/ul.
 * picomole: 30817
 * micrograms: 320

BstXI adapter top
Similar to top adapter above, but this (in conjunction with BstXI bottom) will ligate 2 DNA fragments with a specific BstXI overhang. Info: So I need to resuspend in 194.69ul of 0.1x TE. So weight wise, I will have 246ug in 195ul which is 1.26ug/ul.
 * picomole: 19469
 * micrograms: 246

BstXI adapter bottom
In conjunction with BstXI adapter top, this oligo (when annealed) will ligate 2 BstXI overhanged DNA fragments together. This is the complement of the above oligo with the same 3' overhang. Info: So I need to resuspend in 167.7ul of 0.1x TE. So weight wise, I will have 211ug in 168ul which is 1.26ug/ul.
 * picomole: 16770
 * micrograms: 211

R2342
This is the reverse primer that will allow extension to carry out for an extra 400bp. With this primer I will have 1.5kb PCR products which I will digest at about 1.1kb and gel analyze to determine digestion. Info: So I need to resuspend in 630.04ul of 0.1x TE. So weight wise, I will have 360ug in 630ul which is 0.57ug/ul.
 * picomole: 63004
 * micrograms: 360

BstXI adapter bottom biotin
In conjunction with BstXI adapter top, this oligo (when annealed) will ligate 2 BstXI overhanged DNA fragments together. This is the complement of the top adapter oligo of the same name but with the same 3' overhang. This also has a biotin and a nick for unzipping experiments. Info: So I need to resuspend in 251.56ul of 0.1x TE. So weight wise, I will have 317ug in 252ul which is 1.26ug/ul.
 * picomole: 25156
 * micrograms: 317