IGEM:IMPERIAL/2006/Protocols/bradford assay

Bradford Assay
1) 1 in 5 dilution of Bradford assay solution 2) Use 1mg/ml BSA as a standard protein 3) Place 200 of diluted protein into the well of 96 well plate. 4) Make different dilution of your sample protein and then measure absorbance at 595nm, then calculate concentration using BSA standard curve.
 * 4ml in 16ml of dH2O
 * Small stock in the fridge, main stock in the cold cabinet)
 * (light sensitive)
 * Dilute different amount of BSA (0.1-1)