User:Fermenter User/Notebook/MAUK A9

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

MAUK A9

Day -1 (Mar 23 Mon):
               3/23/2009 14:45 Bring media to fermenter room            3/23/2009 14:50 Assemble bioreactor 3/23/2009 14:50 Add 1 ml of Antifoam            3/23/2009 14:51 Start Sterilization (Liq 45 min) 3/23/2009 00:00 Cooling of bioreactor (Temp setup) 3/23/2009 00:00 Start Fermentation Software for Fermenter #7 Record 0:00 3/23/2009 00:00 Start DASGIP OD module 3/23/2009 00:00 Purge with < 0.5 L/min Air 3/23/2009 00:00 Calibrate dO2 probe 3/23/2009 00:00 Connect Acid (500 ml) and Base (400 ml), prime pumps 3/23/2009 00:00 Start pH control 3/23/2009 00:00 Set dO2

Day 0 (Mar 24 The):
           3/24/2009 00:00 Autozeroing OD probe 3/24/2009 00:00 Completion of bioreactor set up                      3/24/2009 00:00  Inoculation: (?? ml) Fermentation 0:00                3/24/2009 00:00  Sampling Fermentation 00:00 F7: OD (??)                3/24/2009 00:00  Sampling Fermentation 00:00 F7: OD (??)                3/24/2009 00:00  Sampling Fermentation 00:00 F7: OD (??)                3/24/2009 00:00  Sampling Fermentation 00:00 F7: OD (??)  <Sung-Hye>           3/24/2009 00:00  Change temperature <Sung-Hye>           3/24/2009 00:00  Change dO2 <Sung-Hye>           3/24/2009 00:00  Add inducer <Fred>               3/24/2009 00:00  Sampling Fermentation 00:00  Induction 00:00 F7: OD (??) <Fred>               3/24/2009 00:00  Sampling Fermentation 00:00  Induction 00:00 F7: OD (??) <Fred>               3/24/2009 00:00  Sampling Fermentation 00:00  Induction 00:00 F7: OD (??)

Day 0 (Mar 25 Wed):
<Sung-Hye>           3/25/2009 00:00  Havest and Finish Fermentation


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