IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/08/17

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Biofilm (100816M + 100816E) Growth Protocol

 * Using Melody's 3mL cultures in plastic tubes of RN4220 + 8325-4
 * Inoculated flat bottomed 96 well plate with 1% glucose TSB RN42220 and 8325-4 in the below arrangement
 * Both plates were done exactly the same, at different times

Biofilms 100816M + 100816E Arrangement

 * I think Will check with the lab book tomorrow
 * Note: C = control, R = 1% glucose RN4220, 8 = 1% glucose 8325-4
 * 100816M (Melody's plate) went in at 1722
 * 100816E (Eric's plate) went in at 1830

O/N culture for miniprep

 * 3T1,3T2,4T1,4T2 in shaker @ 37C @1632

QS Track

 * Noticed agrCA from distribution came from wrong kit plate (it is on plate 2 instead of 1).
 * Resuspended agrCA in 15 μL dH2O and then transformed into DH5alpha cells using UBC iGEM protocols.
 * Digested and Ligated agrCA (natural Staph form) with B0014 (terminator) onto a PsB1C3 backbone. --Followed Biobrick protocols
 * Digestion time: 30 mins. Ligation Time: 50 mins.
 * agrCA was not purified using a spin column
 * Transform ligation mixture following UBC iGEM protocol. Use all 20 μL.


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