Richard Lab:protocols

Back to Richard Lab Main Page =Equipment=

Dionex IC

 * Overview
 * Eluent Preparation
 * Standard Preparation

Dionex ASE

 * Overview

Anaerobic Chamber

 * Overview

YSI Analyzer

 * '''Overview

=Methods=

Biomass Analysis

 * Silage Protocols
 * Biomass Compositional Analysis
 * NDF (Neutral Detergent Fiber) Determination
 * ADF (Acid Detergent Fiber) Determination
 * ADL (Acid Detergent Lignin) Determination
 * Enzymatic Hydrolysis of Biomass
 * Quantification of Glucose

Enzymatic Screens and Assays

 * Ferulic Acid Esterase Protocols
 * Laccase Protocols
 * Xylanase Protocols
 * Beta-galactosidase (LacZ) Assay
 * '''Beta-galactosidase Screen
 * Beta-glucuronidase protocols

Molecular/Synthetic Biology

 * Amplified Insert Assembly
 * Bio-Brick Formats
 * Richard Lab PCR Protocols
 *  Site Directed Mutagenesis
 *  Plasmid Extraction
 * DNA Quantification
 *  DNA Concentration
 * DNA Synthesis
 *  Restriction Digestion
 * Partial Restriction Digest
 *  Agarose Gel Electrophoresis
 * Polyacrylamide Gel Electrophoresis
 *  Gel Extraction of DNA
 * Ligation
 * Preparation of Shuttle-vectors for transformation
 * Transforming Lactococcus lactis
 * Making Electro-competent E. coli
 * Electro-transformation of E. coli
 * Electro-transformation of L. Plantarum
 * Chromosomal Integration

Manure Storage and Emissions Analysis

 * Measuring and Analyzing Gas-Flux from a Surface
 * '''Setting Up Biofilters in the Field.
 * Formatting Data from Data Logger
 * The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.
 * BOD, moisture and VS content
 * Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.

Compost Analysis

 * Manure and Compost Sample Preparation
 * Chemical Oxygen Demand

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