User:Karmella Haynes/Notebook/Polycomb project/2010/04/26

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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04/26/10

 * &#x2713; Re-transformation: FlpE plasmid (use 20 and 10 μg/mL Amp)
 * &#x2713; Primer order: mCherry internal for RT-PCR of single mCherry fusions

Order primers --> Product length = 100 bp
 * 1) mCh f1 5'-CCCGCCGACATCCCCGACTA
 * 2) mCh r1 5'-GGGTCACGGTCACCACGCC
 * 3) mCh f2 5'-AGCTGCGCGGCACCAACTTC
 * 4) mCh r2 5'-GCGCCGTCCTCGGGGTACAT


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