IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/05/28

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5/28/10
Bootcamp Day 5

9:00

Set up plate for plate reader

Our samples were in lanes 2 and 3, rows B-G, 250 ul of sample in each well.

9:10

Miniprep. 22 ul final volume, used 2 ul to nanodrop.

Concentrations

12M 324 ng/ul 12O 353 ng/ul

10:00

PCR

added 27.8 ul mastermix, .2 ul polymerase, and 2 ul DNA template to each reaction.

Samples: 12M, 12O

Primers: Biobrick Prefix, Biobrick Suffix

Program: iGEM001


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