User:Isis Trenchard/Notebook/BioE44 Stuff/2010/03/27

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Stuff to make for M1:D1

 * finish cloning
 * 002, 003, 004 into no promotor plasmids
 * transform into E. coli.
 * overnight cultures
 * sterile water
 * aspirators need to be set up (for first day actually..)
 * add ethanol to PE
 * aliquot miniprep buffers (into 1.5mL centrifuge tubes)
 * P1 - 250uL
 * P2 - 250uL
 * N3 - 350uL
 * PE - 750uL
 * restriction enzymes... dilute before class? aliquot?


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