User:Karmella Haynes/Notebook/miR Trigger/2010/05/12

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05/12/10

 * &#x2713; CFP reporter lines: check CFP expression

CFP reporter lines > No detectable CFP expression in any of the wells > Discard all plates and start fresh > Investigate alternative strategy --> New neo vector: cut CMV promoter out of pcDNA3.1+ neo (MV1) using SpeI; insert transgene as E/S fragment --> Try transfection of linearized plasmid (in order to maximize inserts with known ends); this may help to control the numbers of functional inserts for gene dosage investigation


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