User:Anand Gupta/Notebook/Microbial Fuel Cell/2009/04/28

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Microbial Fuel Cell
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Overnight Culture Protocol
Overnight cultures are prepared in order to accumulate large numbers of bacteria in a liquid medium, which is useful in maximizing bacterial product yield. In the MFC, we'll be using liquid media at the Anode to allow for H+ ion flow as well as maximum respiratory yield(which will supply electrons for the cathode reduction).

Preflight Checklist

Protocol 2 Materials
 * LB Broth Powder
 * dH20
 * 500ml Flasks
 * E Coli k-12 stock plate
 * 15ml Sterile Tubes
 * Inoculating loops
 * Incubating Shaker
 * Alcohol/Chlorox [disinfectant]
 * Bunsen Burner/Bacti-incinerator

LB Broth Prep

1) Add 6.66g of LB Broth powder to 300ml of dH2O in autoclavable flask, stirring and heating to dissolve.

2) Autoclave flask for ~3hrs

Culture Prep

1) Add 10ml LB Broth to sterile tube.

2) Touch 1 colony on stock plate with sterile inoculating loop.

3) Place inoculating loop in Sample broth and swirl to release bacteria from loop

4) Incubate bacteria at 37C in shaker for ~24hrs.


 * }