IGEM:British Columbia/2009/Protocols/Transformation

Supplies Needed:

 * Competent cells
 * Water bath/heat block at 42°C
 * Ice
 * SOC/LB medium
 * LB agar plates (with appropriate selection)
 * Plasmid DNA
 * Shaking incubator at 37°C

Steps:

 * 1) Remove competent cells (100uL aliquots) from -80°C and thaw on ice.
 * 2) Add 1uL ligation mix to thawed cells and incubate for 30 minutes on ice.
 * 3) Heat shock in water bath/heat block for 60 seconds and put back on ice for two minutes.
 * 4) Add 400uL SOC/LB medium and incubate at 37C for 1-2 hours.
 * 5) Plate one plate each of 100uL and 400uL, and spread evenly with sterile glass beads.
 * 6) Incubate overnight (<18hrs) at 37°C.