IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-3

  Previous Entry  Current Entry  Next Entry  

To-do short term

 * PCR purify the second batch of digested KaiA/B.
 * Gel purify the excised BioBrick backbone (2kb fragment)
 * Ligate the above two products
 * Run the second batch of digested RFP BioBrick plasmid on a gel Failed
 * Gel purify the insert
 * (Maybe) Gel purify the backbone
 * Add phosphatase to the backbone to make it ready for ligation
 * Prepare our mysterious 400b segment for sequencing

Sequencing

 * ZS101: LC1PUR1 w/ crossF
 * ZS102: LC1PUR1 w/ crossR
 * ZS103: LC2PUR1 w/ crossF
 * ZS104: LC2PUR2 w/ crossR
 * ZS105: 400bp (stared) w/ crossF
 * ZS106: 400bp (stared) w/ crossR
 * ZS107: 400bp (circled) w/ crossF
 * ZS108: 400bp (circled) w/crossR

Expected sequences

 * 3-10: 1-210 = 210bp
 * 9-8: 189-559 = 371bp
 * 7-6: 538-2940 = 2403bp
 * 5-4: 2922-3009 = 88bp

Nanodropped amounts (ng/ul)

 * Water: 0.9. 0.7
 * KaiA: 4.1, 4.6
 * KaiB: 1 (before vortexing), 10.5 (after vortexing)
 * BB backbone high copy 1: 10.6 (only curve that looks ok)
 * BB backbone high copy 2: 9.9