User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/26

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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AMPure Cleaned PCR Product
07April10 Ethanol Cleaned Product (~30ul) - Consumed 10ul of remaining Product

- Used Standard Agencourt AMPureXP Protocol http://www.beckmangenomics.com/products/dna_purification_and_cleanup/agencourt_ampure_xp.html


 * Final Elution step, only could retrieve ~30ul cleanly, minimal bead pick up.

PEG Cleaned PCR Product
Clean remaining 07April10 Ethanol Cleaned PCR Product ~18ul with PEG.

To make PEG: 3g PEG 8000 2.19g NaCl Bring up to 15ml with good water. Lasts 30 days

Protocol: 1.	Add 1:1 volume of PEG to PCR product. 2.	Vortex briefly. 3.	Warm at 37degrees for 15minutes. 4.	Microcentrifuge for 15minutes at 4000rpm (cold is best). 5.	Pipette off supernatant. 6.	Wash pellet with 100ul 80% cold ethanol. 7.	Spin for 10minutes at 4000rpm. Cold is best. 8.	Pipette off ethanol. 9.	Repeat steps 6-8 once or twice. 10.	Dry down. 11.	Resuspend in 10ul good water. 12.    NanoDrop