User:Karmella Haynes/Notebook/Polycomb project/2010/09/10

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09/10/10

 * &#x2713; Minipreps: KAH160, 170/015 (3 each); sequencing order
 * &#x2713; p16 induction time course: add dox to 3-day plates (7 pm)

Minipreps/ sequencing > Genewiz sequencing (same day service): template, primer (Note: KAH160-015-01 and KAH170-015-01 are the clones I gave to Casey)
 * 1) KAH160-015-01, CMV-fwd: good
 * 2) KAH160-015-02, CMV-fwd: good
 * 3) KAH160-015-03, CMV-fwd: seq. failed
 * 4) KAH160-015-04, CMV-fwd: seq. failed
 * 5) KAH170-015-01, CMV-fwd: good
 * 6) KAH170-015-02, CMV-fwd: good
 * 7) KAH170-015-03, CMV-fwd: good
 * 8) KAH170-015-04, CMV-fwd: good
 * 9) KAH160-015-01, BGHR: good
 * 10) KAH160-015-02, BGHR: seq. failed
 * 11) KAH160-015-03, BGHR: good
 * 12) KAH160-015-04, BGHR: seq. failed
 * 13) KAH170-015-01, BGHR: good
 * 14) KAH170-015-02, BGHR: good
 * 15) KAH170-015-03, BGHR: good
 * 16) KAH170-015-04, BGHR: good

--> Discard KAH160/015-04 --> Ran Megalign (ClustalW) to find mutations; perfect matches to LTR sequences, no conflicts between clones for good sequences


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