Joyce: DNA precipitation

Concept

 * DNA needs to be precipitated to remove residual salts that may inhibit other reactions (like restriction digestion, PCR, and so on)

Procedure

 * 1) Add enough 3M NaOAc pH 5.2 to make the final volume 0.33 M, vortex
 * 2) Add 2 volumes of 99% EtOH, vortex
 * 3) Place at -70°C for at least 30 minutes
 * 4) Spin at 14K (?) at 4°C for 30 minutes
 * 5) Pipette off supernatant (don't disturb the pellet)
 * 6) Wash (gently invert tube 3-4 times) with 300 ul 80% EtOH
 * 7) Spin at 14K for 2 minutes
 * 8) Pipette off supernant (don't disturb the pellet)
 * 9) Spin down residual EtOH and remove by pipetting
 * 10) Air dry for 20 minutes at 37°C, dessicate or speed vac it
 * 11) Resuspend in sterile water or TE

Links
| Protocol-online thread

Biosize Bio - How Ethanol Precipitation Works