Love:TCMedia:Hybridoma

Formulation of media for Hybridoma cells
This media is for maintaining mouse hybridoma cells (established monoclonal lines or newly expanded clones).

Base media: Dulbecco’s Modification of Eagle’s Medium: 1X, with 4.5 gm/L Glucose, with L-Glutamine, with Sodium Pyruvate (Mediatech #45000-304)

Supplemented with:
 * 10% Fetal Bovine Serum (50 ml) 			(PAA, ###)
 * 50 µM 2-Mercaptoethanol 	(0.5 ml)		(make 50 mM stock in PBS or sterile H2O, filter sterilize)
 * 10 mM HEPES (5 ml) 				(1:100 of 1M stock, Mediatech #15630-080)
 * MEM Non-Essential Amino Acids (5 ml)	(1:100 of 100x stock, Mediatech #45000-700)
 * Penicillin/Streptomycin (5 ml)		 	(100X aliquots in media prep freezer, Mediatech #45000-652)

Mouse monoclonal lines tested:
 * Hyb9901
 * Y3
 * c127
 * c136

(Mediatech products distributed through VWR)