User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/02

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

04/02/10

 * &#x2713; Minipreps: KAH014 (8); KAH96 (4)
 * &#x2713; Sequencing order: KAH014, 96

Minipreps > Check KAH014 with E/P digests; include DPRE w.t. E/P, E/S > Check KAH96 with E/P digests; include KAH86

--> 37°C/ ~15 min.

Sequencing (Genewiz, standard)
 * 1) KAH014-3 P0001: bad (w.t. PstI #1)
 * 2) KAH014-3 P0002: good (mut PstI #2)
 * 3) KAH014-4 P0001: good (mut PstI #1); single bp variation @ 441
 * 4) KAH014-4 P0002: good (mut PstI #2)
 * 5) KAH96-1 P0001: low quality sequence (non-specific)
 * 6) KAH96-1 P0002: good
 * 7) KAH96-2 P0001: good
 * 8) KAH96-2 P0002: good

--> Keep KAH014-4 and KAH96-2


 * }