IGEM:MIT/2006/Notebook/2006-9-12

to do

 * 1) plate reader: 7 tubes in 37 room (amp, a/t, a/k backbone controls) done: dilutions (SP), loading at 5pm (KB)
 * 2) *E 1-3 = J450987 a/k
 * 3) *E 4-6 = R0040-E0840 amp
 * 4) *E 7-9 = J45270 a/t
 * 5) *E 10-12 = osmY-E0840 SN a/t
 * 6) *F 1-3 = J45017 amp
 * 7) *F 4-6 = osmY-Q-E0840 FN a/k
 * 8) *7-9 = osmY-E0840 FN amp
 * 9) colony PCR lots of colonies from the y0078 and y0080 plates: looking for an ATF1 length insert!! done: pcr and LCs (SP) 2 gels (KB)
 * 10) * -- ALL FAILED!!!! boo
 * 11) sequence analysis done: KB
 * 12) *320s are definately correct
 * 13) *all 399s incorrect
 * 14) *397 definately correct for final BB box
 * 15) *1148-mutated J45220 definately correct/successful mutagenesis
 * 16) NOTE: unfortunately, i don't think that the 6 sample sequence order of the 398-muts was ever completed today. since it is way past 5, i moved this task to tomorrow's wiki (KB)