IGEM:PennState/2007/Microcentrifuge tube lists/LW


 * 1) J23038; Transformed 5-23-07 from registry plate 2006-1 well 9G; on plasmid PSB1A2; In -20C Box LW1
 * 2) *RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
 * 3) *[Registry Part Page]
 * 4) J31004; Transformed 5-23-07 from registry plate 2006-1 well 4E; on plasmid PSB1A2; In -20C Box LW1
 * 5) *Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
 * 6) *[Registry Part Page]
 * 7) J31004 A; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
 * 8) *Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
 * 9) *[Registry Part Page]
 * 10) J31004 B; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
 * 11) *Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
 * 12) *[Registry Part Page]
 * 13) J31004 C; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
 * 14) *Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
 * 15) *[Registry Part Page]
 * 16) J23038 A; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
 * 17) *RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
 * 18) *[Registry Part Page]
 * 19) J23038 B; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
 * 20) *RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
 * 21) *[Registry Part Page]
 * 22) J23038 C; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
 * 23) *RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
 * 24) *[Registry Part Page]
 * 25) J23038A; EX(gel extracted) EcoRI+SPEI 5-25-07 from LW006 LW-1;
 * 26) *RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
 * 27) *[Registry Part Page]
 * 28) J23038B; EX(gel extracted) EcoRI+SPEI 5-25-07 from LW006 LW-1;
 * 29) *RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
 * 30) *[Registry Part Page]
 * 31) J23038C; EX(gel extracted) EcoRI+SPEI 5-25-07 from LW006 LW-1;
 * 32) *RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
 * 33) *[Registry Part Page]
 * 34) J31004A; EX(gel Extracted) ECORI+XBAI 5-25-07 from LW0003 LW-1;
 * 35) *Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
 * 36) *[Registry Part Page]
 * 37) J31004B; EX(gel Extracted) ECORI+XBAI 5-25-07 from LW0003 LW-1;
 * 38) *Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
 * 39) *[Registry Part Page]
 * 40) J31004C; EX(gel Extracted) ECORI+XBAI 5-25-07 from LW0003 LW-1;
 * 41) *Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
 * 42) *[Registry Part Page]
 * 43) Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
 * 44) *A newer version of this is available see LW0016
 * 45) Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
 * 46) *Currently used in PCR: experience successful with Pfx and Pfu
 * 47) 50mM MgSO4; filter steralized; prepared 6-??-07
 * 48) *Experience: successful for PCR; use MgSO4 that is in Pfx package when doing PCR
 * 49) *note Pfu buffer already has MgSO4 in it.
 * 50) Sender Construct; MP 7-5-07 from glycerol stock;
 * 51) *attempted use Digest and religate into pSB1AK3: attempt failed
 * 52) *Similar construct with better ribosome binding site on registry plate 2-2007
 * 1) J31004C; EX(gel Extracted) ECORI+XBAI 5-25-07 from LW0003 LW-1;
 * 2) *Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
 * 3) *[Registry Part Page]
 * 4) Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
 * 5) *A newer version of this is available see LW0016
 * 6) Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
 * 7) *Currently used in PCR: experience successful with Pfx and Pfu
 * 8) 50mM MgSO4; filter steralized; prepared 6-??-07
 * 9) *Experience: successful for PCR; use MgSO4 that is in Pfx package when doing PCR
 * 10) *note Pfu buffer already has MgSO4 in it.
 * 11) Sender Construct; MP 7-5-07 from glycerol stock;
 * 12) *attempted use Digest and religate into pSB1AK3: attempt failed
 * 13) *Similar construct with better ribosome binding site on registry plate 2-2007
 * 1) Sender Construct; MP 7-5-07 from glycerol stock;
 * 2) *attempted use Digest and religate into pSB1AK3: attempt failed
 * 3) *Similar construct with better ribosome binding site on registry plate 2-2007
 * 1) *Similar construct with better ribosome binding site on registry plate 2-2007