UCBigem09-groupmtg

  Home  Protocols  Data  Notebooks  ConstructionFiles   References and Papers Parts Database Group Mtg Presentations  About Us </ul> 10-22-09 [[Media:berkigem2009wetPresentation.pptx | The final igem presentation]]

10-09-09 costume idea!: Let's have a robot costume where one person is dressed up and does a robot move every time someone says "robot". -collective brainchild of Susan and Jenn

Wed 10-7-09 Slide Mtg

Intro slide and Needle scFv data

MGFP slide

Friday 9-4-09 Meeting Minutes

Topics covered:

-cel5B data looks too good since most of the constructs had signal -cel3A had readings close to the negative controls -cel9A had some signals that are significant, but not high -some next steps: 1. repeat assay with positive control (which we got from Lane) 2. make constructs without the displayers to test whether the cellulases have function without displayers
 * cellulases - Azo Cm assay data (which can be found here) discussed.

-will run the assay again -if no improvement seen, will go with flocculation
 * BCA(?) assay on mgfp


 * Needle scFv - will FITC conjugate today and will assay tomorrow


 * assembling of parts - mgfp with linkers have been made. Will make cellulases with linkers, needle scFV with linkers, and internal passengers. New stock plates of cellulases are being made.

-currently - fundamental. highlighting: autotransporters, interesting displayed proteins (scFv, enzyme, structural, peptide zippers), automation, number of parts, characterization/improvement/preliminary rules of cell surface display (linkers and displayers) as a tool for future surface display endeavors.
 * Area of focus

Terry: What type of shirts do you guys want for the team? T-shirts, polos... Jenn: Let's get halters!
 * Team names, logos, clever quotes, and other sorts of ridiculous nonscience.

quote of the day: "think outside the cell" -Patrick

Date: 7/9/09 Presentation: Basic Parts update, Strep Assay, and Functional Assays (Susan, Gaby, Patrick) [[Media:7_9_09 presentation slides.ppt]]