IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/07/02

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Biofilm Track: OD Growth Curve
Obtained ON cultures from ambl at 10:30 am (18.5 hours aerated in 32.5C, 180rpm)
 * Prepared culture mix: 25ml B2 broth + 1ml ON culture for growth curve test

Growth Curve Experiment

Time Sample Number OD reading 1 (A) OD Reading 2 (A) OD reading 3 (A) 12:10-1:10pm 1 1.585 1.580 - 1:10-1:40pm 2 2.454 2.468 2.450 1:50-2:20pm 3 4.396 4.426 4.384 2:20-2:50pm 4 5.144 5.174 5.166

Errors:
 * Didn't do 0 time reading: Measurement right after inoculating 1 ml staph into 25ml B2 broth
 * Didn't have growth control while swirling culture flask (B2+staph) in 37 shaker
 * All values are way to high than normal: For OD 600; We want reading around 0.4 for last reading point (3-5 hours after)

Next Step: Redo Growth Curve Experiment


 * Prepared new ON cultures from glycerol stock: (3ml LB + staph), (3ml B2 +staph), (3ml B2 growth control), (3ml LB growth control)
 * Placed new ON tubes into 37 shaker (90rpm) at 4:30pm; To be removed by 10:30pm (Saturday, July 3rd)


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