IGEM:Harvard/2006/Fusion proteins/Oligos

 1. Main Page 2. Lab Notebook 3. Literature 4. Oligos  

Streptavidin primers
 StrepF, forward primer for SA, no start codon


 * 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA [GCTGAAGCTGGTATCACCGG] 3'

StrepR, reverse primer for SA, no stop codon


 * 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT [GGAAGCAGCGGACGGTTTAA] 3'

StrepFS, forward primer for SA w/ start codon


 * 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA [ATGGCTGAAGCTGGTATCAC] 3'

StrepRS, reverse primer for SA w/ two stop codons


 * 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT [TTATTAGGAAGCAGCGGACG] 3'

StrepMF, forward primer for mutagenesis removing XbaI site in SA


 * 5’ CTGAA AGCCGC TACGTTCTGACCGGTCGT 3’

StrepMR, reverse primer for mutagenesis removing XbaI site in SA


 * 5’ ACGTA GCGGCT TTCAGCGTTACCAACAGC 3’

StrepMF2, alternate forward primer for mutagenesis removing XbaI site in SA


 * 5' CTGAA TCGAGA TACGTTCTGACCGGTCGT 3'

StrepMR2, alternate reverse primer for mutagenesis removing XbaI site in SA


 * 5' ACGTA TCTCGA TTCAGCGTTACCAACAGC 3'

StrepHR, reverse primer for SA+His6tag, no stop codon


 * 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT [ATGGTGGTGATGGTGATGGG] 3'

StrepHRS, reverse primer for SA+His6tag w/ two stop codons


 * 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT [TTATTAATGGTGGTGATGGT] 3'



OmpA primers
 OmpAF, forward primer for full OmpA


 * 5' GTTTCTT C GAATTC GCGGCCGC T  TCTAGA G [atgaaaaaga cagctatcgc] 3'

OmpAR, reverse primer for full OmpA


 * 5' GTTTCTT C CTGCAG CGGCCGC T ACTAGT A [ttaagcctgc ggctgagtta] 3'

OmpA46F, forward primer for OmpA starting aa 46


 * 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA [aacccgtatgttggctttga] 3’

OmpA66R, reverse primer for OmpA ending aa 66


 * 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [GCTGCCTTTGTACGGCATAC] 3’

OmpA159R, reverse primer for OmpA ending aa 159


 * 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [GTTGTCCGGACGAGTGCCGA] 3’



Lpp primers
 LppF, forward primer for Lpp


 * 5' GTTTCTT C GAATTC GCGGCCGC T TCTAGA [atgaaagctactaaactggt] 3’

Lpp29R, reverse primer for Lpp ending aa 29


 * 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [CTGATCGATTTTAGCGTTGC] 3’

Lpp78R, reverse primer for Lpp ending aa 78


 * 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [CTTGCGGTATTTAGTAGCCA] 3’

Lpp78RS reverse primer for Lpp ending aa 78 and stop codon


 * 5’ GTTTCTT C CTGCAG CGGCCGC T ACTAGT [TTACTTGCGGTATTTAGTAG] 3’

