IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/28/Shan Shen

14:00 Miniprep the plasmids of T7 promoter, 1-12M, 1-12O. The concentration of the plasmids:

15:00 Digest the plasmids of T7 promoter, 1-12M and 1-12O Digestion System for plasmids of T7 promoter: 20μL

Digestion System for plasmids of 1-12M and 1-12O: 20μL

17:10 Start to digest. 22:00 Double digestion of plasmid of T7 promoter. Digestion System for plasmids of T7 promoter: 20μL

23:25 Electrophoresis to test the digestion efficiency (17:10). The order and the amount of the samples: marker 10μL, plasmids of T7 promoter 10μL, digestion products of T7 promoter 10μL, plasmids of 1-12M 10μL; digestion products of 1-12M, plasmids of 1-12O, digestion products of 1-12O. Results: The plasmids have been cut downright and are suitable for recycling.