IGEM:UNAM-Genomics Mexico/2009/Notebook/H2/2011/06/06

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Plasmid Restriction
The restriction result is the following:



The lanes were as follows:

We can see the plasmids migrated at roughly the same speed. And we see consistency between all L7s and the L6s. The ladder points to a size of around 2Kb. Therefore, I'm concluding that the ligation was unsuccessful as the plasmids still measure the original 2Kb (controls: lanes 1 & 6), and there's no new 1Kb band appearing in addition to the 2Kb backbone.

I'll try to characterize the promoter by ligating it to part BBa_E0240 (2011 Distro, Plate1, Well 12M, pSB1A2 vector) and normalizing against I20260 (2011 Distro, Plate2, Well 17F, pSB3K3 vector).


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