IGEM:metu/2009/Notebook/wound dressing/2009/08/22

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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22.08.2009
1. Isolations of LUXR-RBS(2), Lysis, AHL, BB were made.

2. Digestions of BB,LUXR-RBS AND KGF4 were made. 3. We couldn't digest.

4. Transformations of 1,2,3,4,5,6,7,8,9,10,11 were made. We saw white colonies.

5. Plasmid order: 1- rbs-tetR-dbt   BBa-P0440 2007-plate1-21K 2009-plate1-10I   840bp 2079bp plasmid

2- ptetR-RBS    BBa-J13002 2007-plate2-3F 2009-plate1-BB  74bp 2079bp plasmid

3- rbs-GFP-dbt   BBa-I13504 (EGF4 (GFP)       2009-plate1-22I    875bp       2079 bp plasmid

4- rbs-GFP-dbt BBa-E0240 2009-plate1-12M   876bp 2007 plate 1-16A

5- lae1+pl-rbs-luxr-dbTT-pluXR   BBa-F2622 2007-plate1-6O 2009-plate1-12G  1062bp 2079bp plasmid

6- Pqrr4    BBa-K131017 2009-plate3-8E  275bp 2079bp plasmid

7- pcılamBda-rbs    BBa-K081006 2009-plate2-10H   72bp

8- rbs-cılamBda-dbTT   930bp   BBa-P0451 PaPs (Cl lambda) (S0153) 2009-plate2-1N 2007-plate1-210

9- rbs-mRFPI-dpt  861bp   RFP-BBa-I13507 2007-plate1-16N 2009-plate 1-220

10- lysis 20 M 11- lysis 20K