Klapperich Lab:Photografting to Zeonor Slides

Overview
This protocol describes the procedure for grafting PEG-acrylate to Zeonor slides.

Materials

 * PEG acrylate
 * Ethylene Glycol
 * 2-Dimethoxy-2-acetophenone (DMPAP)
 * Zeonor slides
 * methanol

Procedure

 * 1) Weigh out 35 mg DMPAP
 * 2) Clean plastic slide with EtOH, then rinse with Millipore water
 * 3) Plasma treat surface of plastic slide for 2 min on medium
 * 4) Mix DMPAP with 800$$u$$L Ethylene Glycol and 1200$$u$$L PEG-acrylate in 2mL tube to get 60% PEG-acrylate solution
 * 5) Heat solution in hot water bath at 85&degC until DMPAP crystals have melted
 * 6) Vortex solution
 * 7) Add solution in uniform layer plastic slide
 * 8) Photograft slide in UV Crosslinker for 30 seconds on maximum energy
 * 9) Rinse with MeOH and let dry under hood
 * 10) remove excess poly-PEG acrylate

Contact

 * Aisha