Chang Lab:Notebook/CBE/08/149/2008/12/11

{| width="800"
 * style="background-color: #FFF"|   Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page
 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page


 * colspan="2"|
 * colspan="2"|

Logs

 * Started 8am


 * Reading of absorbance as mentioned on 10 Dec at the 14, 16 and 24th hr.
 * Serial dilution was also done for this 3 time points.


 * Started at 11am
 * Viable cell count conducted for 3-5th hr.
 * 3rd hr -- 42 (X 10^-10) ==> 4.2x10^12 CFU/ml
 * 4th hr -- 272 (X 10^-10) ==> 2.7x10^13 CFU/ml
 * 5th hr -- 220 (X 10^-12) ==> 2.2x10^15 CFU/ml


 * Prepared M9 medium for use on 15th dec for biofilm quantification.
 * Prepare 1X M9 salt by weighing out 12.8g Na2HPO4.7H20, 3g KH2PO4, 0.5 NaCl and 1g NH4Cl dissolved in distilled water to 1L.
 * Add in the M9 Salt MGSO4-- 0.1204g, CaCl2-- 50ml (from 3.7g in 50mL solution), Glycerol-- 5ml from 40% stock.




 * }