IGEM:Peking/2007/Switch-Notebook/2007-9-12

Problem and Warning
Our Kana and Amp plates seems all wrong!!!

I have discarded them!

Ligation
pSB3K3 with so many fragments

Transformation
The previous ligation system.

Also some self-ligation control and some positive transformation aiming at resources of vectors

Re-digestion of all the fragments
We got these fragments from PCR yesterday