User:Kristina M Holton/Notebook 2007 7 3

DNA MaxiPrep C6-C10

Someone turned off shaker in warm room -> cell culture not as turbid as it should be

Checked overnight culture's OD600 at 9:45 -> .369 (need at least .5, .6-.7 desirable)

P1 Buffer: need to bring stock Tris-HCl pH 7.0 up to 8.0; need RNase A

Cells spun down ~ 4:30, stored in 4C overnight

miRNA Extraction and Purification Using 2nd Protocol

Patient: DR

2ml might not be enough QIAzol for tissue grinder -> increased to about 3ml

Did not need first centrifuge step

Good yield RNA (~24ug)