IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/08/04/Rencheng Gao

11:00 Transformation: pcr1+SupD

13:00 Mini-prep: pla+1-12O1~4, pcr+1-12O1~4, plas1

18:30 Double-enzyme digestion: pla+1-12O3, pcr+1-12O1, pla+SupD1

22:30 Double-enzyme digestion: pla+1-12O3, pcr+1-12O1