IGEM:British Columbia/Protocols/Glycerol Stocks

Materials

 * 60% autoclaved glycerol
 * Day/overnight culture

Equipment

 * 1.5 microfuge tube/cryovial

Method

 * Label each tube with: strain, date, Biobrick part number/ assembly number, antibiotic and any other identifying information
 * Aseptically add 0.25mL 60% glycerol to the tube, then add 0.75mL of culture (final glycerol concentration 15%).
 * Vortex briefly
 * Store in -80°C freezer