User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/24

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03/24/10

 * &#x2713; Minipreps: KAH143 (4)
 * &#x2713; Assembly: KAH144
 * &#x2713; Sequencing order: KAH86, 96, 143

Minipreps > Check with E/P digest

Assemblies
 * 1) KAH144: KAH104/(E/S)/540 &#x2713; + (1) KAH142/(E/X)/~5053

> Digests (Fermentas FD)

> Measure conc.'s

--> KAH104(E/S) = 25.8 ng/μL

> Ligations

--> R.T./ ~10 min., Add to 50 μL Z-DH5α

Sequencing order (same day, placed @ 8pm today, results Friday)
 * 1) KAH86 P0001: missing HSVtkTATA-K promoter
 * 2) KAH86 P0002: good
 * 3) KAH96 P0001: missing HSVtkTATA-K promoter
 * 4) KAH96 P0002: good
 * 5) KAH143 P0001: good
 * 6) KAH143 (check NLSstop) P0002: good (NLS stop2 confirmed)


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