Fong:Protocols

Media and Reagents

 * Fong:LB
 * Fong:Antibiotics - Antibiotic stock preparation
 * Fong:IPTG - IPTG stock
 * CTFUD - Rich medium for C. thermocellum. Contact Chris for this protocol.
 * Fong:MJ - Minimal medium for C. thermocellum
 * Fong:TFU - Medium for T. fusca

Equipment

 * Fong:Spectrophotometry and plate reader use
 * Fong:HPLC

General techniques

 * Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
 * Fong:Cryogenic storage
 * Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
 * Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
 * Fong:Electrocompetency
 * Fong:Electrotransformation of E. coli
 * Fong:Transformation of T. fusca
 * Fong:PCR
 * Fong:Quantification of free Cellobiose
 * Fong:MetModel - Analysis and Utilization of metabolic network modeling.

Nucleic acids

 * Fong:Plasmid preparation
 * Fong:RNA gel electrophoresis/Denaturing
 * Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
 * Fong:DNA gel electrophoresis
 * Fong:BioBrick assembly