IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-12-19

Western blot w/8% TrisGly
1:L 2:C(OD.95) 3:BC(OD.95) 4:AC(OD.95) 5:ABC(OD.95) 6:C(OD.95) 7:BC(OD.95) 8:AC(OD.95) 9:ABC(OD.95) 10:L
 * 1: left corner cutout, right square cutout. Interaction

1: sample 0 (t=0) 2: sample 2 (t=60) 3: sample 4 (t=120) etc.. 12: sample 22
 * 2: Oscillation

Prepared more 1:5000 antiC in 50%Odyseey/50%PBST and 1:5000 anti-chicken.

2h antiC + ~5h antiC@4C.

Post antiC wash 3 x 10m 50%Odyseey/50%PBST Post secondary antichicken IR700 wash in 1x10minPBST 1x10minPBST 1x5min PBS Water rinse