User:JeffreyLau/Notebook/2006-6-14

2006/06/14
Biobrick tutorial

More detailed procedure notes are in lab notebook.


 * Began with 12 samples (3x each of negative control, R0010, E0241, E7104). Fortunately our negative controls showed no growth overnight.


 * Made 9 minipreps for the remaining samples. Set aside the E7104 samples (they are a positive control).


 * Performed digests on 2x each of R0010 and E0241. We lost one of our E0241 samples by accident, so we threw out an R0010 sample to balance.


 * Added Calf Intestinal Phosphatase (CIP) to R0010 samples to prevent self-ligation of the R0010 vector.


 * Performed gel electrophoresis on R0010 and E0241 samples.
 * Our e-gel was not entirely successful.

Must be more careful next time.

Notes on using the nanodrop
 * Nanodrop measures concentration of DNA in small samples (1 µL). It is apparently not very accurate.
 * Clean nanodrop thoroughly with kimwipes and ethanol, before and after each use
 * On desktop, "ND-1000"->"Nucleic Acid"
 * Before each set of samples:
 * Calibrate nanodrop with drop of distilled water
 * Calibrate nanodrop with drop of background (solute), e.g. distilled water or elution buffer
 * Add sample to nanodrop, enter name of sample, "Measure"
 * Note: mix sample before placing on nanodrop