IGEM:MIT/2007/Notebook/2007-7-10

Agenda

 * Red Team - Meeting Friday 11am w/ Drew and Tom
 * Metal Contacts
 * email Meinke
 * email Cad grad (mark.ensor@uky.edu)
 * email/call pUT-Mer - Copenhagen


 * Send for sequencing: miniprep of F2620, E1010, B0034
 * design/order Mer Primers
 * design/order FhuA Primers
 * High Copy Plasmids
 * Transform MerPR+MerR plasmids
 * competent cells - Tom?
 * Obtaining untreated polystyrene for filter

Checked Transformation Plates
DH5a in Amp (100µl) - 14 colonies DH5a in Amp (300µl) - 60 DH5a in Kan (100µl) - 4 DH5a in Kan (300µl) - 10

XL-1 in Amp (20µl) - 720 colonies XL-1 in Amp (200µl) - 4800 XL-1 in Kan (20µl) - 220 XL-1 in Kan (300µl) - 800

Innoculated 4 Liquid Cultures of Transformation from XL-1 in Kan (20µl) Plate

 * Used 5ml of LB+Kan
 * Placed in 37C room at around 12pm

Sent out F2620, B0034, E1010 for Sequencing

 * Used samples #2 because those were also used for the digestion/ligation/3A
 * Form 7942
 * Used 3µl of each miniprepped DNA sample, 1µl of VF2/VR, and 8µl H2O (12µl total)