Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-17


 * Nuri Purswani 14:11, 16 August 2009 (EDT):
 * Modelling directions for M0 and M1
 * Browsing through previous igem modelling pages
 * Meeting with Matthieu 2pm to discuss drylab plan

M0:

Assays: Dineka, Kun

Modelling: Nuri

Cloning: James

M1

Assays: Charles

Modelling: Nuri

Cloning: Charles

M2

Assays: Dave (Trehalose), James (Colanic acid)

Modelling: Tianyi

Cloning: Dave (Trehalose), James (Colanic acid)

M3

Assays: Dineka, Kun

Modelling: Dineka, Kun

Cloning: Dineka, Kun

PRIMERS: Royah, James

Royah Vaezi
 * Gel extraction of AK3 vector and B3023.
 * Dephosphorylation of vector and ON ligation to B3023 at 4°C.
 * PCR of RcsB from Top 10 cells. Failed so will try again with different aliquot of Top 10s.
 * ON restriction digests of OtsA, OtsB and Dam.