IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/22

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

June 22, 2010

 * Prepared TOP10 culture for fluorescence readings
 * Read optical density of fluorescence cultures and diluted to a common optical density- RFP and YFP diluted by using 100 ul of cells and 600ul of broth. CFP diluted by using 100ul of cells and 500 ul of broth
 * Read fluorescence with plate reader and subtracted out native fluorescence
 * Received Taq polymerase and new miniprep kit
 * Revision of Experimental design
 * Programmed thermocycler
 * Streaked 20 and 100ul of CFP, RFP, and YFP


 * }