IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/10

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Entry title

 * Miniprepped the 6 samples which were inoculated yesterday viz. E0430(2); I13504(2); I13522(2)
 * Digested all 6 plasmids with E/P.
 * Gel was run.
 * gel order:
 * BLANK; I13522(1A2)(1); I13522(E/P)(1); I13522(1A2)(2); I13522(E/P)(2); I13504(1A2)(1);I13504(E/P)(1); LADDER; I13504(1A2)(1);I13504(E/P)(1); E0430(1A2)(1); E0430(E/P)(1);E0430(1A2)(1); E0430(E/P)(1);LADDER.


 * G set of ligations were done:
 * J13002(2) + E0020 3A (Chl)
 * J13002(2) + E0420 3A (Chl)
 * J13002(1) + E0020 3A (Chl)
 * J13002(1) + E0020 3A (Chl)
 * J23119 + I13504 3A (Chl)
 * K145151 + E0420 3A (Chl)
 * K145151 + E0430 3A (Chl)
 * K145151 + I13504 --- 3A (Chl)
 * S03879 + I13507 3A (Chl)
 * J23119 + E0430 - 3A (Chl)


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