User:Hetmann/Notebook/2006-11-20

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Digestion
Doing the digests, 8 samples ea.

J36335 /sp:
 * 7.6 uL DNA (64.6)
 * 1 uL 10x BSA (8.5)
 * 1 uL NEBuffer 2 (8.5)
 * 0.2 uL SpeI (1.7)
 * 0.2 uL PstI (1.7)

B0015 /sp:
 * 7.6 uL DNA
 * 1 uL 10x BSA
 * 1 uL NEBuffer 2
 * 0.2 uL SpeI
 * 0.2 uL PstI

J36832 /xp:
 * 7.6 uL DNA
 * 1 uL 10x BSA
 * 1 uL NEBuffer 3
 * 0.2 uL XbaI
 * 0.2 uL PstI

J36834 /xp:
 * 7.6 uL DNA
 * 1 uL 10x BSA
 * 1 uL NEBuffer 3
 * 0.2 uL XbaI
 * 0.2 uL PstI

12h@45, 20min@80. in PCR machine in small room. -ZS

labeling
relabeled all the pre-digested plasmids with new labeling convention; put new labeling convention on fridge.

Goals for 11/18

 * 1) *Progression
 * 2) ** Step 1: Midiprep
 * 3) **# B0015
 * 4) **# J04500+KaiB eg.
 * 5) **# J04500+KaiC
 * 6) **# J04500+KaiA+J04500+KaiC
 * 7) ** Step 2: Digestion
 * 8) **# B0015/SP
 * 9) **# J04500+KaiB/XP (J36832)
 * 10) **# J04500+KaiC/XP (J36834)
 * 11) **# J04500+KaiA+J04500+KaiC/SP (J36335)