IGEM:Stanford/2009/Project Homeostasis/sequences/SoxRS

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RBS – SoxR gene – Terminator – Spacer – SoxS Promoter gaattcgcggccgcttctagagaaagaggagaaatactagATGGAAAAGAAATTACCCCGCATTAAAG CGCTGCTAACCCCCGGCGAAGTGGCGAAACGCAGCGGTGTGGCGGTATCGGCGCTGCAT TTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATAT AAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCG CTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGAAA GAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTA GTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGAT TGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCT GGAAGATGAACAAAACTAAtactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata ctagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacaAATCGC TTTACCTCAAGTTAACTTGAGGAATTATACTCCCCAACAGATGAATTAACGAACTGctgcag
 * Sequence: length= 799

cggccgctactagta


 * Forward Primer: gtttcttcgaattcgcggccgcttctagaaagaggagaaatactagatgg
 * Length= 50 GC= 46%   Melt= 66.7C


 * Reverse Primer: gtttcttcctgcagcggccgctactagtacagttcgttaattcatctgttggggag
 * Length= 56 GC= 50%   Melt= 69.5C