Klapperich Lab:Notebook/Lab Meeting Notes/2009/04/09

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9 April 2009 Lab Meeting
† Announcements † Flu R01 † SEPSIS Project †RNA project † COBRA - Do Rhodamine. Leaked. Need to try again. -Need a size specific positive control. PS beads? charged? † Valve Array/Valve building - done. † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR
 * Need posters for Oakridge by Wed. morning.
 * Sonali is working on RTPCR and plaque assays for progress report.
 * Submitted to LOC.
 * Need to get Jurkat RNA to Jeff. He may visit in May.
 * Integration with the glass slide.
 * Prototype next Wednesday.
 * Get PMMA film.
 * Substrates (JZ)
 * Let's get this to a point where we can publish and move on....JZ and CMK to discuss.
 * Colloid experiment.
 * ordered
 * 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
 * try coated PS beads.
 * Paper submitted to LOC
 * Bonding meeting with MIT folks - Alex left some samples with them.
 * Cathie in charge of Bionterfaces Paper1. Cathie has final draft now before it goes to collaborators.
 * designing herringbone groove for better trapping
 * investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
 * IRB still in revision.
 * Cathie read and gave back PCR 1 paper to QQ and MCK
 * Qingqing has written the introduction
 * repeated test of the PCR efficiency for different designs of channel.
 * test PCR efficiency using smaller DNA size (63bp product)
 * analyze PCR product for different temperature using bioanalyzer.
 * will make figures for PCR1 paper.
 * developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)

† RCA/HDA † Senior project † F31: Cochlea
 * Cathie submitted One pager Whitepaper. Waiting for response.
 * convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?
 * Preliminary no-valve design for 25 microliters.
 * 20 more test chips to Sonali (AACC poster).
 * Sonali try to do 10 microliters in the tube
 * Megan
 * HDA on chip. Make simple chip that mixes two things for HDA. Move chip to heater, do HDA demonstration = home run.
 * Plasma helped bonding.
 * EHS wants MSDS stuff.

† Silica Optimization (Lambda):
 * The following will be submitted monday
 * total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
 * Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
 * Emission spectrum for the positive samples split into 2 groups, one with pico green and the other without at 488nm.
 * Chip ran with total of 5ng of NA, pico green using the low range standard curve


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