User:Steven J. Koch/Notebook/Kochlab/2009/04/18/Osmolyte unzipping data 2003/030124 Journal Entries

Data set notes
copied from http://kochlab.org/files/data/Koch_Data/DataSetNotes/030124.txt 1/24/03 5:49 PM Protocol is normal Popping / BGB protocol. However, Popping buffer is made from 2x Popping buffer January 2003. BGB is actually 10 mg / ml ish. DNA is end-labeled from December 5 2001. I diluted 1:2 in BGB 10. BGB made fresh today. Antidig diluted fresh last week. Beads diluted fresh today. Today will be Betaine studies. I will try probably only 1 M betaine 1x popping buffer plus 1:1000 BsoBI 16 amp laser, sum signal at 1.5 V AOD is 243 units. 0158-0159	1M betaine 500 kHz / s; 1:1000 BsoBI added at 4:19 PM 0160	repeating same tether 0161	repeating same tether with reversal 0162-0163	same as 0158-0159; added fresh enzyme before hand 0164	refolding last tether from 0163 0165	back to normal 0166	michelle data 0167-0168	michelle data, 10,000 reference 0169	same tether, michelle trying reversal at 10,000 initial 0170	same tether, michelle trying reversal at 500 initial

0158.ini
(segment-specific comments below) (segment000) (segment001) (segment002) (segment003) (segment004) (segment005) (segment006) (segment007) (segment008) (segment009)

0159.ini
(segment-specific comments below) (segment000) (segment001) (segment002) (segment003)

0160.ini
(segment-specific comments below) SAME TETHER AS LAST FILE (segment000) (segment001) again (same focus) (segment002) (segment003) (segment004) (segment005)

0161.ini
(segment-specific comments below) same tether as last file (segment000) (segment001) (segment002) (segment003)

0162.ini
(segment-specific comments below) (segment000) (segment001) (segment002) (segment003) (segment004) (segment005) (segment006)

0163.ini
(segment-specific comments below) (segment000) (segment001) (segment002) (segment003) (segment004) (segment005) (segment006) (segment007) (segment008)

0164.ini
(segment-specific comments below) (segment000) (segment001) (segment002)

0165.ini
(segment-specific comments below) (segment000) (segment001) (segment002) (segment003) (segment004) (segment005) (segment006) (segment007) (segment008)

0166.ini
(segment-specific comments below) (segment000) (segment001) (segment002) (segment003) (segment004) (segment005) (segment006) (segment007)

0167.ini
(segment-specific comments below) (segment000) (segment001) (segment002) (segment003) (segment004) (segment005) (segment006) (segment007) (segment008) (segment009)

0168.ini
same tether (segment-specific comments below) (segment000)

0169.ini
same tether (segment-specific comments below) (segment000) (segment001)

0170.ini
same tether (segment-specific comments below) (segment000) (segment001)