Clarke:Constructing vectors


 * 1) Streak selective plate from glycerol stocks of E. coli that contain the vector plasmids and incubate overnight. Plates stored at 4 &deg;C.
 * 2) Pick colony from the plate and put in LB + antibiotic overnight.
 * 3) Spin down cells, run miniprep kit to isolate plasmid DNA. Plasmids stored in buffer EB at -20 &deg;C.
 * 4) Take 1 &mu;g of plasmid DNA, digest with restriction enzymes to give sticky ends that match the insert. ''If enzymes are heat inactivated, can store at -20 &deg;C. (?)
 * 5) Separate vector from cutout by gel electrophoresis. Gel slice stored at 4 &deg;C.
 * 6) Use gel extraction kit to recover vector. Vector stored in buffer EB at -20 &deg;C.