User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/01/20

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Collected tdt-Myb virgin females
 * Set up gfp-PCNA on 3 x tdt-Myb virgins.


 * Collected and began dissection/staining protocol with gfp-Myb, rfp-H2AV flies
 * Will stain with anti-bam, anti-sa, and tdt-myb


 * Dissect testes in 1x PBS Solution. Store in eppendorf with 1x PBS until dissections are finished.
 * Fix with 4% Formaldehyde PBS Solution
 * 20 minutes
 * Wash 3 x 5 minutes in 1x PBS-T
 * Incubate 30 minutes with PBS 0.6% Triton.
 * Wash once with 1x PBS-T
 * Block in 2% NGS
 * O/N at 4C


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