BCH092106-Construction of pJ23076-J23079 (J01022 with same RBS spacing as J01122)

E-X-[Ptet]-S-[stRBS][RFP][TT]-P + X-[RBS with spacing from J01122][RFP]-S-P PCR bh001/ca1001 on pSB3C6-J01022 (XbaI/PstI, neb3, ~990bp) Paste into pJ23076 (SpeI/PstI, neb2, 2105bp) Product is pJ23076-J23079