SBB09 Construct42

Construction of Ice Nucleation Repeats (INP repeats) Basic Part PCR Oeh001F/Oeh002R on pBca1256-Bca1346		{2296bp, EcoRI/BamHI} Sub into pBca9145-Bca1144#5			{EcoRI/BamHI, 3171+910,L} Product is pBca9145-Beh001			{} --- eh001F Forward EcoRI oligo for  gtcagGAATTCatgAGATCTaaccacagtgatctaattgc eh001R Reverse BamHI oligo for  gtcagGGATCCactgtcctgcccggccgttg

JCA: the pcr is right, but you have it in the wrong vector. Also, use your M numbers. See revised:

Construction of Ice Nucleation Repeats (INP repeats) Basic Part PCR Oeh001F/Oeh002R on pBca1256-Bca1346		(2296bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5			(EcoRI/BamHI, L) Product is pBca9495AK-M10048     {} --- eh001F Forward EcoRI oligo for  gtcagGAATTCatgAGATCTaaccacagtgatctaattgc eh001R Reverse BamHI oligo for  gtcagGGATCCactgtcctgcccggccgttg