User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/21

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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Alien Gene LongPCR Sequencing Confirmation
Loaded 2.0ul of sample



Alien Gene LongPCR Purification with QIAquick
http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2


 * see page 19.

Long Term Storage Yeast Cultures - Revived from Freezer
Cultures 1001 and 2722

1) Thaw out one tube of culture in glycerol and YM media 2) 50ul streaked on agar (YE + Glucose) plate 3) Incubate at 33C overnight

Results = Good lawns from overnight culture

--> continue preparing long term storage with remaining yeast cultures

Virus 20S
Amplify Colonies 1, 2 and 4

Load 2.0ul sample