20.109(F07): M13.1 and MDS titers

All data on this page was collected by the MIT's Biological Engineering undergraduate students in the Fall 2007 as part of a laboratory class, 20.109. It represents the culmination of an ambitious phage redesign project that began at the start of the term. Redesigned phage were tested for phage titers on a "complete" E. coli genome (MG1655), and on the indicated MDS, described in

Green Team

 * Team members: Arjun Naskar, Patrick Alayon
 * Description of M13.1 phage: p3F17Ag
 * Description of MDS strain: MDS60
 * Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen.

Purple Team

 * Team members:Phillip Samayoa, Justin Tan
 * Description of M13.1 phage: p7p9 unstuff
 * Description of MDS strain: MDS41
 * Any notes about titers presented here: no phage control has plaques

Red Team

 * Team members:Rosa Alvarez, Vivian Hernandez
 * Description of M13.1 phage:p9-YFP
 * Description of MDS strain:MDS42
 * Any notes about titers presented here:no phage control has no plaques, M13K07 not grown at same time as M13.1

Pink Team

 * Team members:Roshini Zachariah and Juliana Rotter
 * Description of M13.1 phage: p3F17Ag
 * Description of MDS strain: MDS60
 * Any notes about titers presented here:negative control had no plaques as expected

Blue Team

 * Team members: Augusto Tentori and Jessica Keenan
 * Description of M13.1 phage:
 * Description of MDS strain:
 * Any notes about titers presented here:M13K07 control not grown in parallel

Yellow Team

 * Team members:Rebecca Kusko and Jessie Wang
 * Description of M13.1 phage:p8BAP
 * Description of MDS strain: MDS60
 * Any notes about titers presented here:

Green Team

 * Team members: Hao Ding and Neha Batra
 * Description of M13.1 phage: p8::BAP
 * Description of MDS strain: MDS12
 * Any notes about titers presented here: Did not get culture for MDS12xM13.1, ctrl showed no plaques (no contamination)

Purple Team

 * Team members:
 * Description of M13.1 phage:
 * Description of MDS strain:
 * Any notes about titers presented here:

Red Team

 * Team members: Scott Pollom and Spenser Skates
 * Description of M13.1 phage: dual p8
 * Description of MDS strain: MDS42
 * Any notes about titers presented here: Negative control had 160 plaques, so our data might be misleading. We used the 10^-8 dilution data to calculate the three PFU/ml values.

Pink Team

 * Team members:
 * Description of M13.1 phage:
 * Description of MDS strain:
 * Any notes about titers presented here:

Blue Team

 * Team members: Matthew Loper and Kat Pak
 * Description of M13.1 phage:
 * Description of MDS strain: MDS43
 * Any notes about titers presented here: negative control showed 62 plaques so there was probably contaimination

Yellow Team

 * Team members:
 * Description of M13.1 phage:
 * Description of MDS strain:
 * Any notes about titers presented here: