Fong:Spectrophotometry

=Overview=

Materials

 * Blank
 * Sample
 * Fingers
 * Spectrophotometer
 * An expensive and fragile cuvette

Procedure
You need at least 120 μL for a good reading; dilute into water appropriately (eg. 20x dilution is 6 μL sample in 114 μL water). Also if you are reading DNA or other sample with a buffer, use a similar buffer at the same concentration in preparing your blank.


 * 1) If the spec's in lamp-off mode, press any button with your finger.
 * 2) Press "Esc" until you reach the main menu.
 * 3) Select the type of reading you are looking for.
 * 4) Enter the correct λ for your sample
 * 5) Add at least 120 μL of your prepared blank to the cuvette.
 * 6) Measure the blank and thoroughly rinse out the cuvette.
 * 7) Add at least 120 μL of your sample to the cuvette.
 * 8) Use the measured absorbance to calculate concentration.
 * 9) Profit.
 * 1) Profit.

Calculation
Take your absorbance at λ and multiply it by the dilution factor and the constant for the sample in question (e.g., CDNA = 50) The number obtained is the concentration in μg/mL.