JCA082006-Construction of lock3i, lock3j (J23071,J23072)



Construction of lock3 and lock3d variants with proper rbs spacing
PCR ca1000/ca1027R on pSB3C6-J01122	(179 bp, EcoRI/SpeI) Sub into pSB3C6-J23018			(EcoRI/XbaI) Product is pSB3C6-J23071, [Ptet][lock3i][RFP] PCR ca1000/ca1027R on pJ23019-J23049	(319 bp, EcoRI/SpeI) Sub into pSB3C6-J23018			(EcoRI/XbaI) Product is pJ23019-J23072, [Ptet]-X-[lock3j][RFP] 