IGEM:IMPERIAL/2006/project/Oscillator/Modelling/Realistic Values

Known

 * Gene expresion: 2 proteins/sec/plasmid
 * Source: Modelling the Lux/AiiA Relaxation Oscillator


 * LuxR Half-Life: 60min
 * Can be varied between 40 and 100min using furones
 * Source: Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover


 * AHL Half-Life: 3 Hours (Conflicting information)
 * Source: Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus
 * Conflict: Cell-Cell Communication by Quorum Sensing sugests AHL half-life is 24 hours


 * aiiA Half-Life: 24hrs
 * Could be halved by degredation tagging
 * Source: Dr Mann


 * LuxI Vmax: 1.1mol HSL/mol LuxI/min
 * Can be increased by over a factor of at least 1000 with addition of SAM and hexanoyl-ACP
 * Source: Acyl-homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein
 * Source: Cell-Cell Communication by Quorum Sensing


 * LuxI Km:
 * 1. For hexanoyl-ACP: 9.6uM
 * 2. For SAM: 130uM
 * Source: Acyl-homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein


 * aiiA Vmax: 68.6 nmol/min per mg = 1.715mol/min per mol
 * Source: The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase


 * aiiA Km: 0.74mM
 * Source: The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase


 * E.coli Volume: 0.88x10^-15 L
 * Source: Institute for Biomolecular Design

Guessed

 * LuxI Half-Life: 60min
 * Reason: Similar to LuxR Half-Life

Needs to be researched

 * Assocaition constant between LuxR and HSL


 * Dissocaition constant between LuxR and HSL


 * How well does LuxR activate Genes?