IGEM:Peking University/2008/Notebook/Group 2/2008/07/25

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PKU_080725_G2_1

 * Frame Shifting mutation-513C514-50μL system-primers 0.75μL each


 * Digestion-1.4μL DpnI-37°C-2h


 * Self circling


 * Enzyme cutting-Gal4wt-ApaI-SpeI


 * Gel extraction


 * Enzyme cutting-pGREG505-lexO-ApaI-SpeI


 * Ethanol precipitation


 * Ligation-Gal4wt-pGREG505-lexO


 * Gel running


 * }