IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/06/22

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Gel Verification
Procedure:
 * Verifying PCR products from last day (PCR-ing genomic preps)
 * See Gel Verification Protocol in "iGEM Training Workshop Molecular Biology 2010"
 * 1) Prepare one 0.8% agarose gel
 * 2) Weigh out 0.8g of agarose and transfer to a 250mL flask
 * 3) Add 100mLs of 1x TBE buffer and swirl gently to disperse the agarose
 * 4) Microwave the mixture on high power until it boils and the agarose is completely dissolved.
 * 5) Allow the solution to cool to 60ºC by incubating in a 60ºC waterbath for about 10 minutes
 * 6) Add the appropriate (5uL per 50mL) amount of stain; in this case: add 10uL of GelStar
 * 7) Pour the cooled agarose into the plate with the appropriate number of combs
 * 8) Wait approximately 20 minutes
 * 9) Transfer 2uL of each PCR sample into new microfuge tubes
 * 10) Add 8uL of dH2O to each PCR sample
 * 11) Use quick and dirty way of loading gel:
 * Pipet 2uL of DNA loading buffer for each of the samples onto parafilm
 * Pipet 10uL of sample, then pipet the DNA loading buffer (2uL) for a total of 12uL, then load onto gel

Note: Used 100bp DNA ladder, a 1 in 20 dilution Machine Conditions: 100V, 45 minutes, 1xTBE buffer Gel arrangement: Note: May have added more water in sample C  -MP Results:

Lab Stuff

 * Autoclaved 10uL x2 boxes of pipette tips, microcentrifuge tubes 1.7mL x2 bins