Template:SBB10 ConstructionFiles JoannaC sbb18

Construction of Zinc Finger Domain (zf-) (sbb18)

Pool jc01 through jc08 and jc17 through jc24, assemble by PCA PCR jc01/jc08 on PCA reaction	(424 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144	(EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb18      {}

jc01    PCA Assembly of sbb17 and sbb18     AGTTCGAATTCATGAGATCTGCACCTAAGAAGAAACGCAAAGTGGGTATCC jc02    PCA Assembly of sbb17 and sbb18     GCCATTGCTGCTGGTACTCCATGGATACCCACTTTGCGTTTCTTCTT jc03    PCA Assembly of sbb17 and sbb18     TGGAGTACCAGCAGCAATGGCCGAACGTCCATTCCAGTGTCGTATTTGCA jc04    PCA Assembly of sbb17 and sbb18     TATCAGAGCGGGAGAAATTTCGCATGCAAATACGACACTGGAATGGAC jc05    PCA Assembly of sbb17 and sbb18     GCGAAAAGCCGTTCGCTTGCGACATTTGTGGACGTAAGTTTGCC jc06    PCA Assembly of sbb17 and sbb18     TCCGCATGCAGATCCGGCATTGGAAAGGTTTTTGACTACCAGTGT jc07    PCA Assembly of sbb17 and sbb18     TTCGCACTCACACAGGAGAAAAGCCTTTCGCGTGTGATATCTGCGGAC jc08    PCA Assembly of sbb17 and sbb18     GTTCAGGATCCACGGAGGTGAATTTTTGTGT jc17    PCA Assembly of sbb18               CGAAATTTCTCCCGCTCTGATAACCTGTCTGTTCACATCCGAACGCAC jc18    PCA Assembly of sbb18               GCAAGCGAACGGCTTTTCGCCAGTGTGCGTTCGGATGTGAACAGAC jc19    PCA Assembly of sbb18               ATCTTCGTATGGTTGATCCGGTGAGCGTTACGGGCAAACTTACGTCCACAAATG jc20    PCA Assembly of sbb18               TCACCGGATCAACCATACGAAGATCCACACTGGTAGTCAAAAACCTTTCCA jc21    PCA Assembly of sbb18               TGCCGGATCTGCATGCGGAATTTTAGCCGTAGTGACACCCTCAGC jc22    PCA Assembly of sbb18               GCTTTTCTCCTGTGTGAGTGCGAATATGTTCGCTGAGGGTGTCACTACGG jc23    PCA Assembly of sbb18               CGGGTTGATCGAGCTGCGAACTTTCGTCCGCAGATATCACACGCGA jc24    PCA Assembly of sbb18               TCGCAGCTCGATCAACCCGTACCAACCACACAAAAATTCACCTCCGTGGATC 

JCA Notes

 * Correct