User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/22

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Let it be!!!
     1. Ladder 
 * Today, I ran a gel to check that the restriction was ok and also to quantify the ligation. 
 * The lanes were as follows:

2. PCR product CBG restricted with ECO RI and SPEI 

3. LRE plasmid restriction with ECO RI and XBA I     
 * I ligated the parts and I replated the colonies from yesterday's transformation.<br/ >


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