User:Anthony Salvagno/Notebook/Research/2009/03/17/D&L Day 4

Today, so far and to do:
 * P/C extraction of overnight digestion
 * EtOH precipitation
 * Make minigel
 * Run minigel comparing digestion with uncut
 * If digestion worked well, we will do the ligation.

Ligation Attempt

 * Allow to incubate at 16C overnight (in PCR chamber thing).

This time the ratios, by weight, are (V:I, vector:insert):
 * 1) V only
 * 5:1
 * 2.5:1
 * 1:1
 * 1:2.5
 * 1:5

Digesting Another Try

 * Add 3.5uL more XhoI after ~2 hours

Mary Ann told Kelly and I we might get better digestion if we use less DNA, so we lowered the amount in the sample.