User:Howard Boland/Notebook/Art from Synthetic Biology/2011/03/08

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Check Transformation

 * 1) 1>12O, XL-1 BLUE, ok
 * 2) 1>12O, XL-1 BLUE, ok
 * 3) 1>12O, DH5-a, blank
 * 4) 1>22I, DH5-a, blank
 * 5) 1>22I, XL-1 BLUE, blank

Overnight Growth

 * 1) 1>12O, E0840, Ok (1.5OD)  -> Stock + Moved 1ml into fresh broth and continue growing
 * 2) 1>22I, I13504, Ok (0.9OD) -> Stock + Moved 1ml into fresh broth and continue growing
 * 3) 1>6I, E0040, appears dark (possible smudging of from plastic in bottle) -> Stock + Moved 1ml into fresh broth and continue growing

Miniprep

 * 1) 5ml 1>12O, E0840 -> Elute in 35µl H20
 * 2) 5ml 1>22I, I13504 -> Elute in 35µl H20

Gel D.D. R0040

 * 1) 10µl 1kb Ladder
 * 2) BLANK
 * 3) 30µl R0040 E/S (H.B.)
 * 4) BLANK
 * 5) 30µl R0040 E/S (A.A.)
 * 6) BLANK
 * 7) 5µl R0040 E/S (H.B.)
 * 8) 5µl R0040 E/S (A.A.)

Gel Plasmids

 * 1) 5µl 1kb Ladder
 * 2) BLANK
 * 3) BLANK
 * 4) 5µl 12O
 * 5) BLANK
 * 6) 5µl 22I
 * 7) BLANK
 * 8) 10µl R0040 (0.5OD>5ml>A.A)


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