McKinney:Chemicals


 * DMSO (dimethyl sulfoxide): liquid in cabinet under chemical hood
 * Kanamycin: powder in shelves above electrophoresis area
 * Hygromycin: 50mg/mL liquid in 4&deg;C deli fridge near hoods
 * X-gal: 50mg/mL liquid in -20&deg;C near hoods. Dilute in dimethylformadide (under chemical hood) to 40&mu;g/mL to make working solution, which should be stored wrapped in aluminum foil (X-gal is very light sensitive) at -20&deg;C.  Use 50&mu;L (~80ng/mL) per plate for blue/white screening of E. coli.