User:Karmella Haynes/Notebook/Polycomb project/2009/11/20

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11/20/09

 * RFP/ Luciferase assay, HEK293 transfectants

RFP/ Luc expression > Transient transfectants from 11/19/09 > Detach cells by pipetting in growth medium (1 mL) > Aliquot 100 μL cells (3 wells per samples 50 - 400 ng plasmid) into ---> A black walled, clear bottom 96-well plate (mCherry reads) ---> A white opaque 96-well plate (luciferase assay) > Use Plate reader protocol "Jake mCherry, OD" for RFP > Add 100 μL Bright-GLO luciferase assay buffer; R.T. ~ 2 min. ---> Use plate reader protocol "Joe luc"


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