User:Tk/Notebook/MF/2008/10/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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 * style="background-color: #F2F2F2" align="center"|  |Main Page


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Plasmid backbones

 * Prefix-RB
 * Suffix-FB
 * templates: pSB1AC3, pSB1AK3, pSB1AT3

J70007, recT gene coding region
gtttcttcgaattcgcggccgcttctagatgaataataaaattgaattaatattaaataattc,recT-F gtttcttctactagtagcggccgctgcagttattaatcagaaaacatttcattaacagc,recT-R gaaatgcaagaagcctatcaacatgaccaagcaataattattaataacagc,recT-mut-F gctgttattaataattattgcttggtcatgttgataggcttcttgcatttc,recT-mut-R

Template: Spiroplasma citri genomic DNA (Renaudin)

858 bp

J70023, MF plasmid origin
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGgccctacttgtttagatagtttaattc,J00023-F GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2

Template: Spiroplasma citri genomic DNA (Renaudin)

1878 bp

J70024, tetM gene coding region
GTTTCTTCGAATTCGCGGCCGCTtctagatgaaaatcataaatatcgg,J00024-F GTTTCTTCCTGCAGCGGCCGCTactagtattattatgtgattttgttg,J00024-R Template: pTT01 plasmid DNA

1920 bp

J70025, tetM promoter region (pBOT1)
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGggccagtctacatgtactc,J00025-F GTTTCTTCCTGCAGCGGCCGCTACTAGTAttcctccattcaaaaacccaa,J00025-R

Template: pTT01

345 bp long

J70026, cat resistance cassette
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGaatagttacccttattatcaag,J70026-F GTTTCTTCCTGCAGCGGCCGCTACTAGTAtctggagctgtaatataaaaacc,J70026-R

Template: pKV104 plasmid (Krause)

1030 bp

PCR results

 * E-Gel 0.8%
 * bands:
 * marker
 * pSB1AC3
 * pSB1AK3
 * pSB1AT3
 * J70023
 * J70023
 * J70024
 * marker
 * J70025
 * J70026
 * J70007
 * marker



PCR purify J70023, 23-F2, 24, 25, 26

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