IGEM:MIT/2006/Notebook/2006-8-9

Smells!!!! : )

 * the banana and wintergreen generating devices are assembled and WORKING!!! YAY!!! what an exciting day :-D

Our New Biobricks!
Let's give a big warm welcome to:
 * 1) J45100 (Prom40+RBS30+BSMT+Term15) [A/T]
 * 2) J45120 (Prom40+RBS32+BSMT+Term15) [A/T]
 * 3) J45170 (PromOS+RBS32+BSMT+Term15) [A/T]
 * 4) J45200 (Prom40+RBS30+ATF1+Term15) [A/T]
 * 5) J45220 (Prom40+RBS32+ATF1+Term15) [A/T]

We will also be updating the registry with all of our intermediate parts as well. Here's a quick outline of our planned number scheme:


 * 1) J45098 (BSMT+Term15) [A/K]
 * 2) J45099 (RBS30+BSMT+Term15) [A/T]
 * 3) J45119 (RBS32+BSMT+Term15) [A/T]
 * 4) J45199 (RBS30+ATF1+Term15) [A or A/K]
 * 5) J45219 (RBS32+ATF1+Term15) [A or A/K]

And, there are always our coding regions...
 * 1) J45001 (SAMT) [A]
 * 2) J45002 (BAMT) [A]
 * 3) J45004 (BSMT) [A]
 * 4) J45008 (BAT2 with SpeI site) [A/T]
 * 5) J45014 (ATF1 with mutation to eliminate EcoRI, but still has a random mutation) [A]

J453xx, 4xx etc will be for the next devices we build.

To do

 * 1) check 42 sample sequencing order in VectorNTI
 * 2) *remember ATF1 parts prob have 2 RBS
 * 3) SMELL LCs (and miniprep plus sequence promising ones)
 * 4) *remember that the ATF1 assembly is a mutant version [J45014]
 * 5) ATF1 mutagenesis (??)
 * 6) make LCs of pUCP22 cell colonies (LB Amp)
 * 7) make LCs of overnight transformants (all in LB A/T, some with SA or IA)

BAT2 mutation primers to eliminate SpeI
Primer pair 3 *   Forward: 5' CGGGCAAGAAGGAACTGGTTACTGCTCCACTAG 3' Reverse: 5' CTAGTGGAGCAGTAACCAGTTCCTTCTTGCCCG 3' *    GC content: 54.55%           Location: 32-64 Melting temp: 80.6°C        Mismatched bases: 1 Length: 33 bp               Mutation: Substitution 5' flanking region: 16 bp   Forward primer MW: 10187.73 Da     3' flanking region: 16 bp    Reverse primer MW: 10080.66 Da