User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/02/03

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Turned over crosses
 * Collected two [MH107,hsFLP/Y ; mRFP, myb+, FRT42D/FRT42D] males.
 * Heat shocked at 37C for 1 hour
 * 11:50 AM - 12:50PM
 * Will dissect on Thurs, Feb 5. with the following stains:
 * Either Spectrin w/ 488 Secondary or Falloidin 488
 * Totpro for DNA
 * Will collect more males on Thurs, Feb 5. and heat shock.
 * Will dissect on Tues, Feb 10.

FRT/RFP Recombination

 * Collected virgins of the three crosses and set up the following cross:
 * V[mRFP/FRT] x [Sc/Cyo]
 * Will collect non-scutoid males with orange-red eyes and set up individual crosses to V[Sc/Cyo].
 * Will collect more virgins Feb 4 and Feb 5.
 * Turned over crosses


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