User:Morgan G. I. Langille/Notebook/Halophiles/2010/08/09

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Genome Rearrangements in Hfx. mediterranei

 * JE forwarded an email from Adit Naor in reference to PCR product suggesting that inversion of large region in Hfx. mediterranei shown in sequencing is not real. [[Media:Mediterranei.pdf‎|Email from Adit Naor (converted to pdf)]]
 * JE and AD referred me to Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei paper that showed the same inversion that we found with sequencing.
 * Possible explanations to why they got their PCR result:
 * They have a different strain and the inversion could have happened recently
 * The inversion is not stable and both could be present in the population (mate pairs in sequence data actually might support both arrangements of the genome)
 * PCR experiment might not be valid. Are the primers unique enough? Negative control?


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