User:Fermenter User/Notebook/MACG F1

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs Purple: Induction hrs

MACG F1 (F3)

Day -2 (Feb 8 Sun)
                2/8/2009 (17:30) Prepare for small culture. (15mL culture from plate)

Day -1 (Feb 9 Mon)
 pH sensor: monitor or control pH   dO2 sensor: monitor or control dO2 Condenser: Gas outlet OD probe: monitor cell growth Triple: Addition of media, Acid or Base if necessary MeOH sensor: Monitor EtOH (byproduct)  MeOH sensors also detects ethanol! :See reference #1 on the bottom of the page            2/9/2009 (9:40)  Pour media 2/9/2009 (9:46) Antifoam added 2/9/2009 (9:57) pH calibration 2/9/2009 (10:14) Autoclave bioreactor 2 empty bottles/fermenter Carbon source+Inoculation ==> Autoclave malfuntion? All media came out of bioreactor 2/9/2009 (12:23) Make media again: 3.4-L (380 ml x10 + Water) 2/9/2009 (12:23) Antifoam added 2/9/2009 (12:25) pH calibration 2/9/2009 (12:40) Autoclave bioreactor 2/9/2009 (14:14) Start Temp Control 2/9/2009 (14:18) Start add rest of media and carbon sources using pumps at Temp 55°C 3.4-L media (autoclave) 1.1-L media 0.25-L Carbon Total 4.75 2/9/2009 (14:41) Start OD software: ended 2/9/2009 (14:44) Start F3 software 2/9/2009 (15:41) Restart OD software

                2/9/2009 (14:05) Prepare small culture for inoculum. (350mL)

Day 0 (Feb 10 Tue)
Sung-Hye: CHBE Class Auditing 9:30 - 11:00

          2/10/2009 (7:30)  Calibrate dO2 2/10/2009 (8:10) Set dO2   2/10/2009 (9:00)  Fermentation 0:00 Inoculation 250 ml each using pump.                2/10/2009 (11:00) Fermentation 2:00 F3: OD (0.465) Contamination? (N)                2/10/2009 (13:30) Fermentation 04:30 F3: OD (0.600) Contamination? (N)                2/10/2009 (15:30) Fermentation 06:30 F3: OD (0.860) Contamination? (N)                2/10/2009 (17:00) Fermentation 08:00 F3: OD (0.980) Contamination? (N) <Ann>               2/10/2009 (17:30) Fermentation 08:30 F3: OD (1.020) Contamination? (N)

Day 1 (Feb 11 Wed)
<Ann>               2/11/2009 (9:00)  Fermentation 24:00  Induction 00:00 F3: OD (5.98) Contamination? (N) Start feeding: 2.7 ml/hr <Ann>               2/11/2009 (13:00)  Fermentation 28:00 F3: OD (5.90) Contamination? (N) Induction 04:00 <Ann>               2/11/2009 (17:00)  Fermentation 32:00 F3: OD (8.42) Contamination? (N) Induction 08:00

Day 2 (Feb 12 Thu)
<Ann>               2/12/2009 (01:00)  Fermentation 40:00 F3: OD (7.28) Contamination? (N) Induction 16:00 <Ann>               2/12/2009 (07:00)  Fermentation 46:00 F3: OD (8.48) Contamination? (N) Induction 22:00 <Sung-Hye and Ann>  2/12/2009 (9:00)   Fermentation 49:00 Added rest of feed solution (~200mL) Induction 25:00 <Ann>               2/12/2009 (14:00)  Fermentation 53:00 F3: OD (9.12) Contamination? (N) Induction 29:00 <Sung-Hye>          2/12/2009 (16:00) Fermentation 55:00  Induction 31:00 Calibrate EtOH <Ann>               2/12/2009 (16:00) Fermentation 55:00  Induction 31:00 Possible Harvest