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Sam Ng

EspP Autotransporter
Source: E. coli 0157:H7 genomic DNA Start by reading PMID: 16262782. You will make a part that begins with amino acid 852 and extends to the stop codon. The DNA sequence for espP is available at: NC_002128.

Myc Tag
Source: pBca1256-K112502   { } This part has already been constructed as a BglBricks basic part in plasmid pBca1256. You will be transferring the part using the Gateway reaction into the assembly vector. To do this, you will react the source plasmid with plasmid pBca1254AK. As background, you should read PMID: 12536251.



This part encodes a passenger protein
After performing the Gateway reaction, your product plasmid will be pBca9495AK-partname. To see what the part looks like in the product vector, look at [[Media:JCA_pBca9495AK-Bca1144.seq | this map]] of the RFP-expressing part Bca1144 in pBca9495AK.