IGEM:University of Chicago/2009/Notebook/PNP Biosensor/2009/08/10

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August 10th, 2009
Run PCR to confirm genomic integration at specific promoter regions.

Dilute Primers for this

Restreak E.coli onto LB/AMP for DNA replication

PCR Design 25 ul RXNs, on bead, one blot of cells

PCR Tube #s

Run PCR using superfolder E-COli as template. Hopefully get superfolder fragments which can be ligated onto the longtin plasmid. If not, I innoculated a couple of Falcon tubes of LB/AMP with superfolder E.Coli

End of the Day