Calendar:ImperialWetLabNotebook2009iGEM/2009-8-24

David Roche 09:28, 24 August 2009 (EDT)

WaaL ligase PCR

 * Used Barnes buffer instead of Pfu Ultra Buffer
 * PCR taken at 48'C, 50'C and 52'C with the negative at 48'C
 * Successful PCR (at last!) at all 3 temperatures. We get the same unspecific band as we have been getting previously (perhaps the sterile water is not so sterile??).
 * will run a restriction digest on the 52'C PCR product overnight, later today.