User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/27

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] RII Overlay
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * RII overlay
 * Activate membrane in EtOH 100% for 15 sec.
 * Rinse with H2O
 * Block in Blocking buffer for 3 hours
 * Blocking buffer (5% Skim milk, 0.1% BSA, 0.0001% Sodium Azide in PBS)


 * RIPA BUFFER (100 mL)
 * 50 mM Tris-HCl (5 mL 1 M Tris pH 7.4)
 * Adjust to pH 7.4
 * 150 mM NaCl (0.87 g NaCl)
 * 1 mM EDTA (37.22 mg EDTA)
 * 1% Triton x-100 (1 mL Tritox x-100)
 * 1% Sodium deoxycholate
 * 0.1% SDS (1 g SDS)


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