IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/11

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Miniprep of RBS-RFP-Terminator, Assembly 1 (J23100 + GFP), pBAD reverse and Amp/Tet Construction Plasmid

 * Day cultures of 5.6mL media and cells innoculated from plates were started at 10:50
 * RBS-RFP-Terminator, Assembly 1 and pBAD reverse were all grown using LB-Amp
 * Amp/Tet Construction Plasmid was grown on LB-Tet
 * Three cultures of Assembly 1 were started because the colonies on the plates did not have a noticible green flouresence
 * Miniprepped using a Invitrogen ChargeSwitch Pro Filter Plasmid Miniprep kit after 7 hours culture
 * Did not observe many cells after being spun down in a Galaxy 16 centrifuge
 * Used 50uL of Elution Buffer instead of 100uL as wanted as high of a concentration as possible as the number of cells is less then usual (was not inncubated as long as usual)
 * Eric started overnight cultures of all 4 plasmids in case we need to do another miniprep tommorow

==Preparation of Miniprep ON Cultures)
 * Innoculated the following cultures of E. coli (done with Charles)
 * Took a colony and innoculated fresh 6 mL LB-antibiotic
 * Incubated at AMBL 37ºC incubator
 * Tomorrow: miniprep the cultures and make glycerol stocks of these.

Ampicillin - New stocks testing

 * 1) Made a new stock of Amp-100
 * 2) 20 mL sH2O + 2.0755 g ampicillin sodium salt
 * 3) Filter sterilized
 * 4) Stored in 2x 15 mL culture tubes (1000X stock)
 * 5) Tested by spread plating 20µL of stock


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