IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/08

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Wednesday 8th September

 * Gel Electrophoresis [] was repeated with the two differentlly cut plasmids containing Part BBa_E0040:

The Gel was set up as follows starting from the bottom: 1) Part BBa_E0040 cut with EcoR1 and Pst1. 2) Part BBa_E0040 cut with EcoR1 and Xpa1. 3) Ladder.




 * Both bands from each lane were removed using the gel extraction kit.[]


 * A ligation was set up between the cholera system and the cut plasmid pACYC. Six different variations of the ligase constituents were used to see which produced the best results:


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