User:Karmella Haynes/Notebook/BioBrick cloning/2009/11/29

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11/29/09

 * &#x2713; Cultures: KAH99(2), 100(2), 101(2), 102(2), 103(2), 104(2)
 * &#x2713; Oligo annealing: KAH012 (phosphorylate, anneal)

Oligo annealing ---> Phosphorylate oligos, then anneal overnight --> 37°C/ 1 hr. --> Add 4 μL 500 mM NaCl --> 100°C waterbath/ 10 min. --> Cool to R.T. overnight
 * 1) KAH012


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