User:Dileep D. Monie/Recipes/Supplemented M9

Supplemented M9 Media (500 ml)
NOTE: This is the same as the Endy Lab version but does not require preparation of intermediate solutions
 * Mix the following components, allow to dissolve completely, and sterilize with a 0.2 μm PES sterile filter
 * 497 ml of ddH2O
 * 50 μl of 1M CaCl2 (Teknova C0477)
 * 1 ml of 1M MgSO4 (Sigma M3409)
 * 5.64 g of M9 minimal salts, 5X (Sigma M6030)
 * 150 mg of thiamine hydrochloride (Sigma T1270)
 * 1 g of casamino acids (Acros Organics 61204)
 * 2 ml of glycerol (Invitrogen 15514)
 * Protect Supplemented M9 Media from light and store at 4°C