User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/04/16

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Friday 16th April 2010
I checked the PCR using a gel, but it came out negative in both samples of the PCR and negative control, being positive only in the positive control.



First and last lines are the molecular weight ladder.

2º => positive control

3º and 4º => PCR samples

4º => negative control

next two => Augusto’s

next four => Mariana’s 17D, 18D, 30D, 18D respectively.

As Paz PCR’s didn’t come out well too, we hypothesize that the problem is the DNA amount, so I’ll transform cells next Monday and Mariana will try the PCR again.


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