User:Hana Benzeer/Notebook/SGM Summer Project/2011/06/29

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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inoculation
2 parts to inoculate using transformed DH5-α
 * 1) 2 10ml tubes for each (4 total)
 * 2) Label the tubes
 * 3) Turn on the flame
 * 4) I15008, add 50μL,Ampicillin
 * 5) I15010, add 50μL, Kanamycin
 * 6) Inoculate 2 plates into each 2x 10 ml tubes.
 * 7) 4 tubes are then left in the shaker overnight

Check overnight growth (10x100ml)

 * 1) Take the tubes.
 * 2) Remove 700μL from each tube and transfer into eppendorf tube.
 * 3) Add 300μL of 50% glycerol stock into each eppendorf tube.
 * 4) Mix by vortex.
 * 5) Store in -21°C freezer.
 * 6) Spin the 10ml tubes for 10 min at 70.
 * 7) Remove the supernatant
 * 8) Store the tubes in -21°C freezer.