Terrific Broth

Summary
Terrific broth (TB, not to be confused with T Broth)

Ingredients

 * 1) 12 g Bacto tryptone
 * 2) 24 g Bacto yeast extract
 * 3) 4 mL Glycerol
 * 4) 100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution.

Protocol

 * 1) Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL
 * 2) Pour into 2 L flask (or greater)
 * 3) Autoclave (liquid cycle)
 * 4) Allow liquid to cool to less than 60 degrees Centrigrade
 * 5) Add 100 mL potassium phosphate solution