User:Marcel Quint/Notebook/medicago/2008/04/26

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

check competent cells - negative control
use this protocol - link still missing
 * use competent cells only as negative control, dissolve in 3 ml LB and plate the following dilutions on LB kan/spec plates: 2-5-50μl + spin down, dissolve in 150μl and plate everything → check on mon

result from 4/24 - empty pRedroot transformation = positive control

 * transformation of electrocompetent cells with empty pRedroot worked fine no matter which amount was plated → use 5 and 50 µl for the future (agro and ec2 electroporator programs both fine)


 * }