IGEM:IMPERIAL/2007/Calendar/2007-9-6

We are in Week 9  of the iGEM project

Project Calendar
name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7

GFP problem

 * Experimental design team had a meeting with Kirsten and Frank regarding the problem
 * Have decided to concentrate on characterising DsRed Express
 * All constructs will be cloned with DsRed Express and tested
 * Should be able to start experimenting on DsRed Express constructs by start of week 11
 * In the mean while will carry on looking for purified form of GFP mut3B

Meeting with Paul Freemont

 * 12pm tomorrow
 * Each team to make an agenda to talk on
 * Must talk about what has been achieved in the past few weeks - no formal presentation

Problems with experments

 * 24 hour access to lab and fluorometer to get better results as staggering not giving good results
 * Freemont will arrange for a fluorometer that can be used 24 hours
 * No temperature control so can only carry out tests at room temperature and degradation experiments

Data analysis

 * Dry lab team waiting for better results/data from the excperiments
 * Current data not very useful - 24hr experiments would be very useful
 * Currently writing a program to carry out data analysis

Cell extract

 * Homemade cell extract was tested with pTet and pT7 constructs
 * Results seemed ok but fluorescence was seen in the negative controls too in a fluorescence microscope
 * Possible reasons - plates, the cell extract or the reaction mixture is contaminated
 * Will carry out more tests on homemade cell extract to figure out where the fluorescence is from

Vesicles

 * Characterising emulsion process - refining protocols to improve the process of vesicle formation
 * Trying to come up with techniques to quantify the process of vesicle formation

French iGEM team members visit

 * Hopefully the following people should be able to show them around and talk about the project:
 * Lucas
 * Alex
 * Anthony
 * Cheuk

Other issues

 * Dry lab team should be free from modelling and data analysis by end of this week
 * Can join the vesicles team and/or the experimental design team
 * A log book placed with the fluorometer
 * Need to write down full details on it about all the experiments being carried out - for record