Jessica Karen Wong/Notebook/2007-7-30


 * Sent for seq: all promoter testers cut w/ not1 (I2057 and E0240 S&X versions)
 * Diluted BB_R_Rev_Eco, BB_R_Rev_Spe, and BB_I2056_Fwd_X primers each to 40uM
 * BB PCRed both samples of I2056 to make both Eco and Spe versions (4 100ul rxns, 3:50ext time)
 * 10ul PCRed both preps of I2055 and the cell suspension of Col #1 with GFP internal Rev primer and each VF and VR
 * Checking to see if part is flipped, if flipped GFP_Rev and VR will show, if not GFP_Rev and VF will show
 * Testing Top10 competent cells by transforming and plating puc18
 * Made overnights of promoters J23116 and J23100 to miniprep, digest, and then test using E0240 and I2057
 * Transforming E0240-3K3-E/S, E0240-3K3-E/X, I2057-3K3-E/S, I2057-3K3-E/X with Knight Lab's DB3.1 electrocompetent cells
 * Ran a gel of today's PCRs loaded: I2056 Col #1 E, I2056-2-E, I2056-1-S, I2056-2-S, sp lad sp, I2055-3K3 GR &VF (1, 2, 1 cell), sp, I2055-3K3 GR&VR (1, 2, 1 cell)