Endy:STN Chemostat Design

Contact Caitlin Conboy, Jennifer Braff.

This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.

Also see Endy:Chemostat for an alternative chemostat chamber design and additional info on running chemostats.

Design Images


Components
This page is in progress

Equipment

 * Circulating Water Bath
 * VWR
 * Peristaltic pump
 * |Masterflex L/S peristaltic pump from Cole Parmer. Which pump, specifically?
 * Bubbler (Acquarium bubbler)

Glassware

 * 1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
 * Rubber caps for 1L flasks with two drilled holes
 * Glass tubing
 * 25mm glass test tubes, one per chemostat chamber

Tubing, Connectors

 * Silicone tubing
 * Connectors, various, specify
 * Three-way stopcock
 * Cole Parmer luer fittings, chrome-plated brass three-way stopcock

Other

 * Needles, 2 per chamber
 * 18 gauge deflected point non-coring septum penetration needles (Popper).
 * Tube Rack (to hold the chemostat chambers upright in the water bath)
 * USA Scientific
 * Syringes, to innoculate and sample from chemostat

Operation
See Endy:STN Chemostat Protocol