IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Design

 Specifications Design Modelling Implementation Testing/Validation  

Registry

 * 2 parts have been designed to fullfil the specifications:
 * a monocystronic: BBa_J37035
 * [[Image:J37035 parts pic.PNG]]
 * a polycystronic: BBa_J37036
 * [[Image:J37036 parts pic.PNG]]

Design choices

 * We need to fulfill all the properties listed in the specifications.
 * Based on the Quorum-sensing/quenching due to biochemical properties needed and BioBricks availability.
 * Following the design of the  Prey-construct, the prey molecule is an homoserine-lactone (AHL).
 * Due to the biochemical constrains, the predator has to be decoupled into 3 modules:
 * Sensing module driven by the dimer formed between AHL and LuxR and its affinity to the pLux promoter.
 * Killing module driven by the use of the lactonase aiiA.
 * Natural death of the predator molecule.

Open issues

 * Testing should be done to distinguish between the monocystronic and polycystronic construct (maybe their transfer functions are different)
 * If the degradation rates of LuxR and aiiA are different, it will introduce an asymmetry in the system.
 * The aiiA BioBrick in the Registry has never been properly tested and validated.

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