User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/05/06

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 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
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May 6th, 2010
  -H2O --> 11μL -Buffer ---> 3μL -DNA -> 15μL -Phosphatase --> 1μL  
 * Previous to ligation, I dephosphated the double terminator | BBa_B0015 with the following reaction for 30μL:
 * The reaction was incubated at 37°C for 20 min and at 65°C for 10min.
 * I observed a clear band by electrophoresis in a 8% agarose gel.


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