IGEM:MIT/2005/Friday 9/7

Updates
This is a new article that seems like a relevant and interesting review of antibody engineering, describing scFvs in particular

General

 * There's a microscope in TK's lab in addition to Drew's
 * Access to bld.68, lab and office off hours - will be done Wed. when Drew gets back
 * Lab space: Got it all worked out, I'll show everyone around after the meeting.

Input-Maxine
None

Head Unit-Jen

 * BurningMan was awesome
 * Will continue to work on scFv-test construct & testing
 * Personally, would like to help with more urgent aspects of project

ToxR-Will

 * Assemblies
 * Been using 3-drug method


 * ToxR is toxic in cells?

FecA-Annie

 * Construction of FecA Pro-RFP, FecA Pro-GFP
 * Verifying step


 * FecA'
 * Tried 3-step PCR method but last step failed
 * Gel diagnosis suggests connection step fails --> advisors?
 * PLAN: redo 3rd step


 * Fur & FecA KO
 * PCR CmR and TetR using new primers, only has CmR about right size, TetR controls indicated primer dimers
 * PLAN: Proceed with Fur KO
 * Redesign primers for PCR TetR


 * Sequences have come in
 * scFv-Linker is correct

Planning

 * Scheduling
 * -With Advisors
 * meeting - change date and time
 * Lab moving


 * -With Team
 * meeting - date and time
 * Team coordination