DNA Precipitation

Overview
This protocol can be used to concentrate DNA, or to change the buffer the DNA is suspended in. It can also be coupled with phenol chloroform extraction for the purifying nucleic acids. This protocol also works for RNA precipitation (take care to use RNAse free materials in this case).

Materials

 * 3M NaOAc pH 5.2
 * EtOH 95%
 * Glycogen (optional)

Procedure

 * 1) Add 0.1 volumes of 3M Sodium Acetate solution to 1 volume of DNA sample.
 * 2) Add 1ul Glycogen to the DNA sample.
 * 3) Add 2 volumes of 95% EtOH to the DNA Sample.
 * 4) Store the solution overnight at -20&deg;C or for 30 minutes at -80&deg;C.
 * 5) Centrifuge the solution at maximum speed for least 15 minutes.
 * 6) Decant and discard the supernatant.
 * 7) (Optional) Add 1 ml of 70% EtOH to the pellet and let sit for 5 minutes.
 * 8) (Optional) Centrifuge the sample at maximum spped for 5 minutes.
 * 9) (Optional) Decant and Discard the supernatant.
 * 10) Air-dry the pellet for 10-15 minutes at room temperature until all liquid is gone.
 * 11) Resuspend in desired volume of water or buffer

BioCoder version
Following is the DNA Precipitation protocol in BioCoder, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioCoder (see Source code). More information about BioCoder can be found on my home page. Feel free to mail me your comments/ suggestions.Vaishnavi

Text Output
DNA Precipitation protocol

Source Code
DNA Precipitation protocol - source code

Links

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Contact
Discuss this protocol.