User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/12

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Summary

 * Membranes were washed 3x 15min. separately depending on secondary antibody
 * α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
 * Old school imaging
 * Preparation of 2x 10% tris-glycine gels --> Put to 4°C


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