Imperial College/Courses/Spring2008/Synthetic Biology/Part Measurement and Characterisation

 Wet Lab: Part Measurements and Characterisation

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Instructors: Geoff Baldwin, Kirsten Jensen, Duo Lu, Vincent Rouilly

Date: 25th, 26th, 27th, 28th, 29th February 2008

Venue: Wet Lab, RSC1 building.

<font face="trebuchet ms" style="color:#FFFFFF" size="3">Objectives:
 * Part Characterisations (data collection + analysis):
 * Constitutive Promoters
 * Inducible Promoter
 * Log/Stationary Phase detector (from iGEM MIT 2006)
 * GFP calibration curve
 * Wet Lab Skills:
 * E.Coli culture + monitoring growth (OD600)
 * Fluorescent measurement from a 96-well plate reader
 * Calibrated Fluorescent Measurement.
 * Data collection and analysis (lab notebook).
 * Sharing your data.

<font face="trebuchet ms" style="color:#FFFFFF" size="3">Deliverables:
 * Lab Notebook on OpenWetWare
 * Report due on ...

<font face="trebuchet ms" style="color:#FFFFFF" size="3">Announcements:


 * Registration to OpenWetWare required ( join OWW}.

<font face="trebuchet ms" style="color:#FFFFFF" size="3">Acknowledgments:
 * Thanks to Jason Kelly  (MIT) for providing us the DNA plasmids, and a great deal of feedbacks.
 * OpenWetWare folks for creating such a wonderful Open Science platform.