IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/13/Shan Shen

Electrophoresis to test the digestion products of 1-18A The order and the amount of the samples: marker 5μL, plasmids of 1-18A 2μL, digestion products of 1-18A 20μL. Results: The digestion products still run slower than the uncut plasmids, but 1-18A is tested to be gel-bad according to the registry, so that the products are probably correct.

10:30 Continue to prepare competent cells.

13:50 Ligation of the 1-18A vector and 1-12O (GFP) inserts.

22:30 Transformation of the ligation products and 1-1D, 1-6I to test the efficiency of the competent cells.