IGEM:IMPERIAL/2007/Notebook/2007-9-10

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/10 view=threemonths format=%name/%year-%month-%day weekstart=7

Vesicles
Results

Sample 8, produced on 04.09.2007 was looked at again under the fluorescence microscope, but this time only a single 2&mu;m vesicle was found.

Preparations

Three POPC/dodecane 10ml suspensions were prepared.

Cloning of Biobricks (for Maxiprep)

 * 1) Placed 100 ml of LB in a 500 ml conical flask
 * 2) Innoculated 1 ml of culture into LB media in flask
 * 3) Incubated overnight at 37 °C


 * 25. BBa_J37032 [pLux-GFP]
 * 13. BBa_B0015 [ ]

Protocols can be found at Maxiprep in the general protocols page

Transformation of Biobricks

 * 1) 1 biobrick was transformed into XL1-Blue
 * 2) Cells were grown for half an hour and plated
 * 3) Plates were incubated at 37&deg;C overnight


 * Transformed in XL1-Blue
 * pSB1A3 [pSB1A3] (re-clone)

Protocols can be found at Electroporation in the general protocols page