Colorimetric

TNBS Test - Primary Amines
Solution 1: 10% DI(P)EA in DMF (store at 25o C) Solution 2: 1 M aqueous TNBS (store at 4o C) Procedure: - Place a few resin beads in a small test tube. - Add 1-3 drops of each solution - Mix, and leave at room temperature for 10 minutes

Kaiser Test - Primary Amines
WARNING: Ambiguous with Wang resins Solution 1: 5 g ninhydrin in 100 mL ethanol (store at 25o C, in dark) Solution 2: 80 g phenol in 20 mL ethanol (store at 25o C, in dark) Solution 3: 2 mL 0.001 M KCN in 98 mL pyridine (store at 25o C, in dark) Procedure: - Place a few resin beads in a small test tube. - Wash resin with ethanol 2-3 times to remove adsorbed DMF - Add 2-5 drops of each solution - Mix, heat to 100o C for 5 minutes

Chloranil Test - Primary Amines (acetaldehyde) - Secondary amines (acetone)
WARNING: Keep solution for a maximum of one month Solution 1: 2% acetaldehyde (primary amines) or 2% acetone (secondary amines) in DMF (store at 4o C) Solution 2: 2% chloranil in DMF (store at 4o C) ALTERNATIVE SOLUTIONS Solution 1: Saturated solution of chloranil in toluene (store at 4o C) Solution 2: Only necessary for primary amines. 2% acetaldehyde DMF (store at 4o C) Procedure: - Place a few resin beads in a small test tube. - Add 2-5 drops of each solution - Mix, leave at room temperature for 5 minutes

Bromophenol Blue Test - Primary and Secondary amines
WARNING: Ensure resin is well washed with DMF before performing this test Solution 1: 0.05% bromophenol blue in DMA (store at 25o C)

Procedure: - Place a few resin beads in a small test tube. - Add 10-20 drops of solution - Inspect immediately

Isatin Test - Secondary amines
Solution 1: Saturated isatin in benzyl alcohol with BOC-Phe-OH Preparation: - Saturated isatin (~0.4 g) in benzyl alcohol (~12 mL) - Stir for ~2 hours at room temperature, filter remaining isatin - Mix 10 mL of filtered solution with 0.5 g BOC-Phe-OH

Procedure: - Place a few resin beads in a small test tube. - Add 3-4 drops of solution - Heat to 100o C for a 1.5 to 2 minutes (MAXIMUM)