IGEM:Brown/2007/Lab Protocols/Pouring Plates 3

For the creation of Agar Plates:

Materials: -Distilled Water -LB (E. Coli Nutrients) -Agar (Gel Substance) -Ampicillin

Plan: -Make Stock Solution of Ampicillin -50 mg ampicillin/mL water -Final Solution should be 100ug/mL

Procedure: 1. Make 10 mL of Stock Solution -10 mL * 50 mg/ml= 500mg Ampicillin 2. Make LB Solution. -20g LB/L Water -make .5L of LB Solution 20g/L * .5L= 10g LB. 3. Agar-LB Solution -20g LB/L Water -20g Agar/L Water -Make .5L Solution 4. Autoclave in container 1/4 full of solution. -Make sure top is covered in aluminum foil. 5. Let cool to 50 C while stirring. 6. Add 1 mL stock to each solution. 7. Make Plates.