User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/03/03

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Maize Endophyte Biofertilizers
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The Daily Grind

 * To do today:
 * Plate out all soil plant endophyte glycerol stocks and incubate for 10 days (dilute 10 ul glycerol in 90 ul water and spread)
 * Gel purify digested PCR and plasmids and ligate overnight
 * Extract DNA from all the frozen soil plant samples and spec
 * Analyze second rep of TRFLPs from seeds

The E. coli survival experiment will have these specs:

Two E. coli - Empty BAC 2O2 and Initially positive BAC 1O1 Three dip treatments - colony resuspention in LB, O/N in LB, and O/N in LB with antibiotics Sampling of the entire plant by grinding and plating on LB + chloramphenicol + XGal. Two reps of this. Sampling times will be Just after dipping, Day 1, Day 3, Day 7, Day 14. OD600 of all innoculants will be taken


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