Van Oudenaarden Lab:B0304.1

Progress:

Probes designed: (03-01-2010 - Alexander van Oudenaarden)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

>B0304.1a (hlh-1 spliced coding region)

atgaacacggaaacctcaactcaatcggcgcccagtgacacctacgacaccagcatctactacaactcgagtccacgtgt caccgcaaatgacatcaccacactgacaagtttcgctgcgccagctccacaagttcttgattatgccaatacgcagtatg atatctatagaaaccagccagcttactacctcccgtcctatgctccaactgcacctaccactttctactcggatttcgcc aatttcaatgtgaccagatctcaagatttcgcatcagttccagccgtggccaactcgtcagacgtgaagccaatcattat caaacaggaaaagagcacaccaaatgccacagagctcataattcagagccgagtcgacagtcaacatgaggacactacaa cgtctactgctggcggagccggtgtcggtggaccgcggagaaccaaattggacagacggaaggcggcaacaatgcgtgag agacggcgtcttcggaaagtcaacgaagcttttgaagttgtgaagcaaagaacgtgtccgaatccgaatcaacgtcttcc taaagtcgagattctcagatcagcaattgattatatcaataatctagaaagaatgctgcaacaagccggaaaaatgacaa agattatggagcagaaccagcatttgcagatgacacagcagattaatggagctccacctcacgattatgtgacttcctct cactttgcatcgagctcttacaatccagaaaatatgtttgacgatgatgatttgacggattcggacgacgatagagatca tcataagctcgggaacgcggtcgatctgcgtcggcgaaactctttggacagattgtcgcggatcgttgcgagcattccca acgaggaagcaatgaccgacgaacaactcctccaaccagcaaatgatgtgattgatggggagaaaaaacttgagatgctc tga

Name of probe set: hlh-1

Probes designed on Mon, 01 Mar 10 14:21:10 -0700

43 probes designed for target of length 963 (target too short for 48 probes)

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

gttgaggtttccgtgttcat, hlh-1_1, 1, 1, 45

tgtcgtaggtgtcactgggc, hlh-1_2, 2, 30, 60

ctcgagttgtagtagatgct, hlh-1_3, 3, 52, 45

tgtcatttgcggtgacacgt, hlh-1_4, 4, 75, 50

gcgaaacttgtcagtgtggt, hlh-1_5, 5, 97, 50

aagaacttgtggagctggcg, hlh-1_6, 6, 119, 55

catactgcgtattggcataa, hlh-1_7, 7, 141, 40

gctggctggtttctatagat, hlh-1_8, 8, 163, 45

agcataggacgggaggtagt, hlh-1_9, 9, 185, 55

agaaagtggtaggtgcagtt, hlh-1_10, 10, 207, 45

ttgaaattggcgaaatccga, hlh-1_11, 11, 229, 40

gaaatcttgagatctggtca, hlh-1_12, 12, 251, 40

tggccacggctggaactgat, hlh-1_13, 13, 273, 60

attggcttcacgtctgacga, hlh-1_14, 14, 295, 50

gctcttttcctgtttgataa, hlh-1_15, 15, 317, 35

tgagctctgtggcatttggt, hlh-1_16, 16, 339, 50

ctgtcgactcggctctgaat, hlh-1_17, 17, 361, 55

cgttgtagtgtcctcatgtt, hlh-1_18, 18, 383, 45

caccggctccgccagcagta, hlh-1_19, 19, 405, 70

ttggttctccgcggtccacc, hlh-1_20, 20, 427, 65

tgccgccttccgtctgtcca, hlh-1_21, 21, 449, 65

gacgccgtctctcacgcatt, hlh-1_22, 22, 471, 60

aaagcttcgttgactttccg, hlh-1_23, 23, 493, 45

cgttctttgcttcacaactt, hlh-1_24, 24, 515, 40

gacgttgattcggattcgga, hlh-1_25, 25, 537, 50

ctgagaatctcgactttagg, hlh-1_26, 26, 559, 45

attgatataatcaattgctg, hlh-1_27, 27, 581, 25

gttgcagcattctttctaga, hlh-1_28, 28, 603, 40

atctttgtcatttttccggc, hlh-1_29, 29, 625, 40

caaatgctggttctgctcca, hlh-1_30, 30, 647, 50

cattaatctgctgtgtcatc, hlh-1_31, 31, 669, 40

acataatcgtgaggtggagc, hlh-1_32, 32, 691, 50

tcgatgcaaagtgagaggaa, hlh-1_33, 33, 714, 45

catattttctggattgtaag, hlh-1_34, 34, 737, 30

ccgtcaaatcatcatcgtca, hlh-1_35, 35, 759, 45

tgatctctatcgtcgtccga, hlh-1_36, 36, 781, 50

gaccgcgttcccgagcttat, hlh-1_37, 37, 803, 60

aagagtttcgccgacgcaga, hlh-1_38, 38, 825, 55

aacgatccgcgacaatctgt, hlh-1_39, 39, 848, 50

cttcctcgttgggaatgctc, hlh-1_40, 40, 870, 55

aggagttgttcgtcggtcat, hlh-1_41, 41, 892, 50

caatcacatcatttgctggt, hlh-1_42, 42, 915, 40

atctcaagttttttctcccc, hlh-1_43, 43, 937, 40