IGEM:The Citadel/Notebook/Notes Brian/05-30-10

May 30

 * Prepared 500mL of SOB for the Electroporation on Monday. Needs to be pH tested and converted to SOC.
 * Prepared 500mL of LB Lennox (see below).
 * Reviewed protocols for training sessions this coming week.
 * Designed a genetic circuit for team's review tomorrow during the long wait for culture growth.

This is a continuation of our work from 05-29-10:

Procedure

 * 1) Autoclaved all flasks and tubes. The 250mL tubes were autoclaved with caps off, so to ensure sterility we've stored them on ethanol-soaked paper.
 * 2) Prechilled the rotor at 4&deg;C. All tubes and pipets will be chilled tomorrow morning upon arrival.
 * 3) Inoculated 5mL LB Miller medium and placed in incubator for overnight growth at 37&deg;C with 240 RPM. Austin Che calls for LB Lennox to lessen salt content. We had only LB Miller at the time of inoculation and have attempted to use that; we'll post an update! For tomorrow's 450mL culture, we've prepared 500mL of LB Lennox.