User:Fermenter User/Notebook/BROM B8

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM B8 (F6)

Day 0 (Nov 17 Mon):
           11/17/2008 12:30 Assemble bioreactor             11/17/2208 13:00 Bring media to fermenter room Change #1: Media composition: JR (C): Expect pH change (will require more Base)            11/17/2008 13:20 Start Sterilization (Liq 45 min) 11/17/2008 15:00 Add 5 ml of Antifoam 11/17/2008 15:17 Cooling of bioreactor (Temp setup) 11/17/2008 15:18 Purge with 0.5 L/min O2                          11/17/2008 15:20 Start Fermentation Software for Fermenter #6 Record 0:00 11/17/2008 15:30 Connect Acid (500 ml) and Base (400 ml), prime pumps Start pH control pH of media was close to 7.0 11/17/2008 15:40 Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 11/17/2008 15:45 Set dO2 11/17/2008 15:50 Completion of bioreactor set up: Ready for inoculation Change #2: Minimum rpm is requested: rpm set at beginning:250             11/17/2008 16:20 Inoculation: (240 ml)  Fermentation 0:00

Day 1 (Nov 18 Tue):
11/18/2008 (8:10) Base on controller record, O2 tank rant out Fermentation 15:50            11/18/2008 (9:00) Open another O2 tank Fermentation 16:40 Acid (475ml) Base (240 ml) Bioreactor picture taken             11/18/2008 (12:00) Sampling Fermentation 19:40 Cell weight (95.8 mg/ml), Activity ( 90 %, x100), Activity ( 58 %, x1000)

Day 2 (Nov 19 Wed):
           11/19/2008 (10:20)     Fermentation 42:00 Acid (405 ml) Base (230 ml)             11/19/2008 (11:00) Sampling Fermentation 42:40 Cell weight ( 171 mg/ml), Activity ( 88 %, x100), Activity ( 48 %, x1000) <Vincent>            11/19/2008 (16:00) Sampling Fermentation 47:40 Cell weight ( 167 mg/ml), Activity ( 93 %, x100), Activity ( 60 %, x1000) <Sung-Hye>           11/19/2008 (16:00)    Fermentation 47:40 Increase rpm to 450

Day 3 (Nov 20 Thu):
<Sung-Hye>           11/20/2008 (8:30)     Fermentation 64:10 Acid (400 ml) Base (230 ml) <Vincent>            11/20/2008 (11:00) Sampling Fermentation 66:40 Cell weight (183 mg/ml), Activity (94 %, x100), Activity (72 %, x1000) <Sung-Hye>           11/20/2008 (11:00)     Fermentation 66:40 Havest and Finish Fermentation


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