Wittrup: E. coli Media

LB Media [see also LB]

Liquid, 1000 mL pH to 7.5 with NaOH (optional?) Add ddH2O to final volume. Filter sterilize, or autoclave. Add antibiotic if selective media is desired (perhaps 100 mg/L amp), but do not add until cooled to below 50 oC (touchable by bare hand)
 * 10g Bacto tryptone
 * 5g Bacto yeast extract
 * 10g NaCl

Plates, 1000 mL
 * Ingredients as above (still save antibiotic for last)
 * 15-18g agar
 * Autoclave. Add antibiotic if selective media is desired, but do not add until cooled to below 50 oC (touchable by bare hand)

Ampicillin [see also Ampicillin]
 * Stock: 25 mg/mL of sodium salt of ampicillin (amp) in water.
 * Filter sterilize and store in aliquots of 4 mL at -20 oC
 * Working concentration = 35-50 mg/L for liquid media. Often 100 mg/L for plates.

SOC Media [see also SOC]

100 mL

Add ddH20 to final volume. Filter sterilize, or autoclave.
 * 2.0g 2% Bactotryptone
 * 0.5g 0.5% Yeast extract
 * 10 mM NaCl (0.2 mL 5 M)
 * 10 mM MgCl2 (1.0 mL 1 M)
 * 10 mM MgSO4 (1.0 mL 1 M)
 * 20 mM Dextrose (D-glucose) (0.36 g)