User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/27

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Nuclear Gene Adenita 454 Amplification
Test AV10027 Primer set on Instagene Extractions

Loaded 2.0ul



Nuclear Gene Adenita 454 Test Chelex Extraction
1. Adenita cultures 5-15 individuals. 2. Spin for 1 min at 12-14,000 rpm. Remove supernatant leaving 20-30ul, careful not to disturb pellet. 3. Add 200ul 20% chelex (Or add chelex until ~1/2 tube full of beads). Vortex, then quick spin. 4. Boil for 10 minutes. 5. Before use, vortex, then spin at 13000 rpm for 3 minutes. Use supernatant right above beads.

Load 2.0ul



Nuclear Gene Adenita 454 Test Chelex Extraction
If the short version doesn’t work, try the long version:

1. Adenita cultures 5-15 individuals. 2. Spin for 1 min at 12-14,000 rpm. Remove supernatant leaving 20-30ul, careful not to disturb pellet. 3. Add 200ul 20 % chelex. (Or add chelex until ~1/2 tube full of beads). Vortex, then quick spin. 4. Incubate at 55 degrees for 1 hour 5. Vortex for 10 seconds. 6. Boil for 10 minutes. 7. Before use, spin at 13000 rpm for 3 minutes.

Load 2.0ul