User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/04

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM 2011
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=Objective=
 * Transformation of plasmid pUX19- XRK5.

Tranformation
This transformation has a pUX19- XRK5 plasmid developed to know the site repC length an his antisense RNA. Has the minimal lenght of insert with antisense sequence and CDS of repC.

Izquierdo, J., Venkova-Canova, T., Ramirez-Romero, M. A., TellezSosa, J., Hernandez-Lucas, I., Sanjuan, J. & Cevallos, M. A. (2005). An antisense RNA plays a central role in the replication control of a repC plasmid. Plasmid 54, 259–27


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