SIL082506-Construction of pSB1AK3-J23074

 [RA2 Promoter][RBS][TraGr][TT] Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 ) Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229) Product is pSB1AK3-J23074.

JCAnderson 20:11, 16 September 2006 (EDT)Revised construction:

Construction of pSB1A2-J23074
[Pcon][stRBS][traG][TT] Digest pSB1AK3-J23026. . . . . . . . (XbaI/PstI, small-2072 bp) Digest pBca1020-J23038. . . . . . . .(SpeI/PstI, large-2121 bp) Product is pSB1A2-J23074 