IGEM:PennState/2006/Growthmedia

Growth Media LB Media (per liter) Plates
 * 10 g Bactotryptone
 * 5 g Yeast Extract
 * 10 g NaCl
 * pH to 7.0 with NaOH/HCl
 * Autoclave
 * Add 15 g Bacto-Agar per liter media
 * Autoclave for 25 minutes
 * If antibiotics necessary, add only once media has cooled to ~45ºC, or is comfortable to the touch
 * Pour in sterile environment (~15ml per plate)
 * Cool in such a way as to avoid accumulation of condensation, while maintaining sterility
 * Seal with parafilm, place in bag, and store at 4ºC

LBM Media (per liter) Plates Top Agar (per liter)
 * 10 g Bactotryptone
 * 5 g Yeast Extract
 * 5 g NaCl
 * 0.4066 g MgCl2.6H2O (or 2mL 1M MgCl2)
 * pH to 7.0 with NaOH/HCl
 * Autoclave
 * Add 15 g Bacto-Agar per liter media
 * Autoclave for 25 minutes
 * If antibiotics necessary, add only once media has cooled to ~45ºC, or is comfortable to the touch
 * Pour in sterile environment (~15ml per plate)
 * Cool in such a way as to avoid accumulation of condensation, while maintaining sterility
 * Seal with parafilm, place in bag, and store at 4ºC
 * 500 mL LBM
 * 500 mL DI H2O
 * 8 g Bacto-Agar
 * Autoclave

SOB Media (per liter) Final Concentration:
 * 20 g bactotryptone
 * 5 g bacto-yeast extract
 * 0.5 g NaCl
 * 0.19 g KCl
 * pH to 7.0 with NaOH/HCl
 * Autoclave for 25 minutes
 * Add filter-sterilized MgCl2 and MgSO4 to yield final concentration of 10 mM each
 * 0.5% Yeast Extract
 * 2% Tryptone
 * 10mM NaCl
 * 2.5 mM KCl
 * 10 mM MgCl2
 * 10 mM MgSO4

SOC Media
 * Add filter-sterilized glucose to SOB for 20 mM final concentration of glucose

Minimal Media M9 Salts (per liter) Media (per liter)
 * 1. Make M9 salts
 * 2. To make M9 Salts aliquot 800ml H2O and add
 * 64g Na2HPO4-7H2O
 * 15g KH2PO4
 * 2.5g NaCl
 * 5.0g NH4Cl
 * Stir until dissolved
 * Adjust to 1000ml with distilled H2O
 * Sterilize by autoclaving
 * 3. Measure ~700ml of distilled H2O (sterile)
 * 4. Add 200ml of M9 salts
 * 5. Add 2ml of 1M MgSO4 (sterile)
 * 6. Add 20 ml of 20% glucose (or other carbon source)
 * 7. Add 100ul of 1M CaCl2 (sterile)
 * 8. Adjust to 1000ml with distilled H2O