Christiaen:Methods

page in construction

this page provides access to the methods referred to as in the beginner's guide to ascidian developmental biology as well as other resources.

animals and embryos
sea water tank and adult animals Most of the methods are described in details in the CSHL press - Ciona protocols, including : dissection and fertilization dechorionnation Ciona electroporation microinjection fluorescence activated cell sorting (FACS)

molecular biology
molecular cloning microarray analysis