Yu:Coomasie blue

{| border="0" cellpadding="10"
 * +Solution recipe for Coomassie Blue( for mass spec,too).
 * 1. 45% Methanol
 * 2. 10% Acetic Acid
 * 3. Water
 * 4. Brilliant Blue G-250( 0.05% final or 250mg per 500ml of the solution)
 * 5. Stir to dissolve, filter with 0.2uM
 * 4. Brilliant Blue G-250( 0.05% final or 250mg per 500ml of the solution)
 * 5. Stir to dissolve, filter with 0.2uM
 * 5. Stir to dissolve, filter with 0.2uM
 * 5. Stir to dissolve, filter with 0.2uM