User:Karmella Haynes/Notebook/BioBrick cloning/2011/01/11

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01/11/11

 * &#x2713; Assemblies: KAH171-173 (re-build miR sensor constructs, might be a problem with old polyA, DDB82)
 * &#x2713; Transformation: MI127 (for minipreps)

Assemblies > Re-build miR sensor using new polyA (from Mara, MI127) and miRNA or siRNA luc RNAi target sites (from Joe) > Re-build RFP repressor construct for testing new polyA (no HS4 insulators)
 * 1) KAH171: (1) JDS46/(S/P)/~3387 + (3) MI127/(X/P)/225
 * 2) KAH172: (2) JDS47/(S/P)/~3398 + "
 * 3) KAH173: HPK/(E/S)/516 &#x2713; + KAH73/(E/X)/~5638 &#x2713;

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

--> 10 min./ R.T.; add to 30 μL DH5α turbo


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