IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/28

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Results from previous days

 * Result of the gel


 * Lane3 : hyperladder1
 * Lane4 : I746101
 * Lane5 : I746001



We wanted to check the size of our biobricks. For I746101, we have a band of about 3000kb, which is the size of the vectorm and a band of about 2000kb. The size of the biobrick is 2057kp, so it should be ok.

For I746001, we hqve the same band of 3000kb (the vector) and a band of about 1000kb. The size of the biobrick is 896kb.

So, the size of our biobricks are ok.

Wet Work
- Digest


 * 1) For biobricks I746001 and I746101, use digest enzymes EcoRI and SpeI, and 2μL of DNA
 * Add 15μL of SDW, 2μL of 10X Fast Digest Buffer, 2μL of DNA, 1μL of EcoRI and 1μL of SpeI
 * 1) For ppL82 (from plate and tube), use digest enzymes BamHI and PstI and add 15μL of DNA
 * Add 2μL of SDW, 2μL of 10X Fast Digest Buffer, 15μL of DNA, 1μL of BamHI and 1μL of PstI
 * 1) Microfuge tubes
 * 2) Incubate 10 min at 37°C
 * 3) Heat shock for 5min at 80°C

- Gel : add 4μL of dye and 21μL of samples


 * New plates

-Plate I746001 and I746101 on Kn plates from Amp plates from 24/07/2008.


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