IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/20

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Advertisement

 * 6th June Logo must be finished.
 * Mr.Gene in facebook. Like.

WetLab

 * Protocols for Luciferase Light quantification.
 * Probably we´ll get TrpLp by genomic DNA from K12, Luciferase by PCR.
 * Probably EnvZ mutant could be in Insituto de Biotecnología.

Goals

 * Get Luciferase to mutate and blue promoter.

Modeling
Δ[Luciferina]=½*F(R, [Luc]) - R([Luc],[Luciferin])- D([Luciferin]). Formula.
 * We use in parameters for Luciferin just:

Where: R= Luciferase Reaction

D=Degradation

Goals
We will get the data of Kms & Kcats, and finalize the Luciferin Model.

General

 * Deadline. Theoretical framework for wiki 8th June.
 * The orders of strains are done.
 * Primers for Lux AB in Vibrio Fischery.