Shreffler:Notebook/Staff Meetings/2010/08/03

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] ShreffLab Staff Meetings
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Agenda Items

 * EoE
 * EDN ELISAs
 * Sarita has list from Marcella and will order a kit or two to evaluate
 * Component proposal -- WS still needs to work on
 * Basophil
 * Review data from last week (Sarita, Alex sens #7,8)
 * Sarita's experiment low baso counts, but good Ag response
 * evidence of pre-existing Derp2 sensitization; however, clear evidence of stronger degran with 50:50 sens than 99:1 or 1:99
 * Next experiments
 * Repeat with buffy's this week, consider lengthening stripping time?
 * Caitlin paper? -- Bert has looked?
 * no discussion today
 * Allergen Adjuvant
 * Review outcome of meeting w/ S. Carr
 * Willing to perform digest/ MS and see
 * Review most recent PCR data on dose response to fraction 3
 * strong evidence of correlation with prominent band on dilution experiment -- very nice response, much stronger in Fr3 than whole CPE as well
 * F/U with Carr
 * Bert will take gels over THIS week
 * Forski's?
 * decent number of CD45+DR+CD1a+, but no evidence of Aledflour enhancement; staining/ flow not optimal
 * PM Baso
 * nothing new
 * JAX
 * quote for peptide arrays
 * WS has to do
 * train Stephanie for project role
 * U19
 * Discuss involvement with Bert

Other Discussion

 * Isabelle will see bakery owner on Thu; working on dietary guideline doc, will meet w/ her tomorrow morning

Things To Do

 * 1) Investigate EDN assay options/ request samples w/ standards (Sarita)
 * 2) Write up component proposal (Marcella and Wayne)
 * 3) obtain quote for peptide arrays
 * 4) send Bert U19 slides
 * 5) send Isabelle Dowling the dining services director name/ contact