IGEM:IMPERIAL/2007/Notebook/2007-8-13

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/15 view=threemonths format=%name/%year-%month-%day weekstart=7

Preparation of Stock Solutions

 * 1x 1 litre of 2xYT medium in Duran bottle
 * 3x 1 litre of 2xYT medium in conical flask
 * Sent to autoclave
 * Solution A for preparation of vesicles

Transformation of Biobricks

 * 1) 7 biobrick were transformed into XL1-Blue or BL21(DE3)
 * 2) Cells were grown for 1 hour and plated
 * 3) Plates were incubated at 37&deg;C overnight


 * Transformed in XL1-Blue
 * BBa_J5528 [pBad-GFP] (re-clone)
 * BBa_E0240 [GFP]
 * BBa_I13507 [RFP]
 * BBa_T9002 [ptet-luxR-pLux-GFP]
 * BBa_B0034 [RBS]
 * Transformed in BL21(DE3)
 * BBa_I13522 [ptet-GFP]
 * BBa_E7104 [T7-GFP]

Protocols can be found at Electroporation in the general protocols page

Pilot Preparation of Vesicles

 * 1) Followed the desiccation and suspension stages of the vesicle preparation protocol
 * 2) One 100ml beaker was prepared with 125&mu;l of DOPC in 50ml of mineral oil
 * 3) The suspension was sonicated for 30mins and left overnight in a 27&deg;C incubator