User:Andrew Perry/Lesser Known Methods in Bacterial Protein Expression

= Lesser known methods in recombinant bacterial protein expression =

Here are a few lesser known methods / protocols / tricks I've collected over the years. I've love to know if any of them are actually useful for anyone.

Self cleaving TEV protease fusion partner


 * doi:10.1110/ps.041129605

Thermostable fusion partners

PMID 14711496
 * doi:10.1016/j.jbiotec.2003.10.008

Elastin tagging

Some elastin-like peptides undergo a soluble–insoluble phase transition ("precipitation"), which can be modulated by salt concentration. When used as a fusion partner, it has be shown that this property can be used as a simple purification step. It appears many methods relating to the use of elastin-like peptides may be patent encumbered.
 * doi:10.1110/ps.04931604
 * doi:10.1002/pro.157