JCA111006-iGEM2006Construction100

Construction of lock3m with 5' extension and proper RBS spacing
Basically, this is J23071 with the 5' extension PCR ca1028F/ca1001 on pJ23019-J23071      (865 bp, XbaI/PstI) Sub into pJ23076                          (SpeI/PstI, large 2105) Product is pSB3C6-J23090, [Ptet][lock3m][RFP]