User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/20

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=Objectives=
 * PCR from extractions
 * Primers (5 pM/μL) (new set because we are improving new protocol of standardization).
 * Primer Forward= CGG GCC CCA TTA TTA TCA TGA CAT TAA CCT ATA AAA ATA GG
 * It includes GGGCCC. Site for ApaI.
 * Primer Reverse= CGA GCT CGC CTT TTG CTC ACA TCT TCT TTC C
 * It includes GAGCTC. Site SacI.

PCR

 * DNA was obtained from extractions and diluted(2μL DNA in 98μL H2O)

Expectatives

 * See in gel amplification of 3 extraction and not amplification in controls.