User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/03

{| width="800"
 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Single rotifer extraction for population genetics

 * Trying to single rotifer-extractions using fusion primers designed for population genetics

PCR conditions

 * 3 min @95C, (5s 95C, 1 min 60C)x35 on Takara machine
 * using MIDs 1 through 12
 * rotifers from Gushikawa 01
 * each placed with 5-10 ul water into a PCR tube. Then 30ul instagene were added and incubated 30min at 56, 30min at 95C, vortexing occasionally

Results

 * Pretty much blank gel. There was some weak amplification in the last sample, but it looks like the extractions have failed.
 * Need to optimize single-rotifer extraction