Hartwell's complete (HC) medium

10x HC dropout solution, per liter, autoclave

 * Methionine 200 mg
 * Tyrosine 600 mg
 * Isoleucine 800 mg
 * Phenylalanine 500 mg
 * Glutamic acid 1000 mg
 * Threonine 2000 mg
 * Aspartic acid 1000 mg
 * Valine 1500 mg
 * Serine 4000 mg
 * Arginine 200 mg

10x YNB, per liter, autoclave

 * Yeast nitrogen base (without amino acids or ammonium sulfate) 14.5 g
 * Note: this results in 15% less of the vitamin/salt mix than Difco YNB w/o amino acids.
 * Ammonium sulfate 50 g


 * Alternatively, Yeast nitrogen base (without amino acids) 57.1 g
 * Ammonium sulfate 7.4 g

1x amino acid solutions, separate, autoclave, per liter

 * Uracil 1 g (0.5 g  in 500 ml)
 * Adenine 1 g (0.25 g  in 250 ml)
 * Lysine 10 g (2.5 g   in 250 ml)
 * Tryptophan 10 g (1.0 g in 100 ml)
 * keep in light blocking bottle
 * Leucine 20 g (2.0 g  in 100 ml)
 * Histidine 10 g (1.0 g  in 100 ml)

Final HC medium

 * Autoclave 20 g of agar in 619 ml water
 * Add 100 ml 20% sterile glucose solution
 * Add 100 ml 10x YNB solution
 * Add 100 ml 10x HC dropout solution

Optionally add

 * Adenine solution 20 ml
 * Lysine solution 12 ml
 * Leucine solution 4 ml
 * Histidine solution 2 ml
 * Tryptophan solution 8 ml
 * Uracil solution 35 ml

Bring the final volume to 1 liter