User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/06

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 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The package is here!!!
  1 LuxCDABE under pBad promoter (269.5 ng/µl) 
 * Today, I received the package that Cambridge team sent to us. I quickly got to work on it and continue to work in my mutated luciferase by transforming everything as follows:

2 enhanced Photinus pyralis luciferase (EPIC) and P. pyralis LRE with no promoter (142.1 ng/µl)

3 enhanced Photinus pyralis luciferase (EPIC) and P. pyralis LRE under pBad promoter(187.8 ng/µl)

4 Luciola cruciata luciferase and L. cruciata LRE with no promoter (203.1 ng/µl)

5 Luciola cruciata luciferase and L. cruciata LRE under pBad promoter (176.4 ng/µl)

6 Mut-luz in pSB1C3

7 Mut-luz with J23102

8 LRE with J23100 (Positive control)   
 * I plated everything but the mut-luz with J23102 (ampicilin) and the LRE in chloramphenicol plates. And I left them incubating at 37°C ON.


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