IGEM:IMPERIAL/2006/LabCalendar/2006-8-26

The Optical density mesuring device in the lab is broken !! Use Perkin Elmer Victor instead

Cryogenic Stocks
Grew up fresh day cultures for freezing at 9.30am
 * T9002
 * J37016
 * J37015
 * S01656
 * J37015RS
 * J37020

Using this protocol, we froze 6 different parts that we are testing


 * Made up 10 Eppendorf tubes of S01656 with 15
 * Made up 30 Eppendorf tubes of T9002 with 15
 * Made up 25 Eppendorf tubes of J37015RS with 15
 * Made up 20 Eppendorf tubes of J37015 with 15
 * Made up 16 Eppendorf tubes of J37016 with 15
 * Made up 20 Eppendorf tubes of J37020 with 15


 * Transferred them to the -80 freezer (in Dr.Mann's section on 6th floor biochem.)

Testing
JohnChattaway 05:15, 26 August 2006 (EDT)
 * Set up fresh cultures of T9002 and J37016, we didn't make a J37020 last night

Overnight culturing

 * 2ml of T9002 for testing
 * 2ml of J37016 for testing
 * 2ml of J37020 for testing
 * 2ml of J37024 (1) for miniprep
 * 2ml of J37018 for miniprep
 * 100ml of J37024 for maxiprep
 * 100ml of J37018 for maxiprep
 * 100ml of 4G for maxiprep