IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/26

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Thursday 26th August:

 * Attempted PCR of BarA a second time but again results are gel electrophoresis were poor.
 * Repeated gel electrophoresis [] of the cut pgaA promoter which produced better results:




 * The now isolated pgaA promoter was extracted from the gel.[]
 * Set up an over night restriction digest of pACYC using Hind3:

Plasmid- 10μL Hind3 - 2μL buffer - 2μL BSA (x20) - 1μL H2O - 3μL


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