IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/08/02/Rencheng Gao

11:30 Pouring plates: LB+Amp ×30, LB+Kan ×50

15:30 Mini-prep: pla1~5, pcr1~4 Gel electrophoresis

17:00 Double-enzyme digestion (front insert) Plasmid: pla1, pla2, pcr1, pcr3

22:30 Gel purification (pcr1, pla1, pla2)

00:00 Ligation: Insert: pcr1, pla1 Vector: SupD, 1-12O