User:Niepelm/Notebook/ICBP45/2010/09/23

{| width="800"
 * style="background-color: #EEE"|[[Image:niepelm_01.png|128px]] ICPB45
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

MCF7 PNNL-15cm
--> problems with the dishes
 * all cells are dead and floating
 * plated in Corning 15cm dishes
 * 2 of the same cells in the same dishes in Mirra's incubator look better but still sick
 * 2 of the same cells in BD Falcon dishes in Mirra's incubator look just fine

--> problems with the incubator?

Order a new stack of incubators dedicated to this project.

MCF7 RPLA-96well

 * cells are attached but don't look great
 * don't use because of problems with the same cell line

HCC-70 SS-15cm

 * some cells are attached but many float and form strange large clumps
 * don't use

HCC-1569/HCC-1954 density

 * treated by Lili

HCC-1187 CNI-96well

 * scanned by Lili

HCC-1954 CNI-96well

 * added primary Ab o/n by Lili

HCC-38 plating controls

 * plate same cells into corning and BD Falcon dishes
 * grow in three different incubators
 * asses growth in the morning
 * test for differences in plates and incubators


 * }