Registry/Measurement kit/Notebook/2007-6-26

To do

 * 1) Gradient scarring PCR on E0240 (and I2055) and run gel (done)
 * 2) Spin down device cultures/replate (done)
 * 3) Run gel on PCR product from yesterday (done)
 * 4) Cleanup yesterday's digests and PCR products (done. in Measurement Kit box)
 * 5) Make new Tet plates (done)

Preparation of 3K3

 * Mini-prepped remaining 4mL of 3K3 (glycerol) culture
 * Concentration was 44.1 ng/µL
 * Digest of 3K3 running overnight.
 * (23µL DNA + 19.5µL H2O)

Analysis of PCR of I2055

 * Gel had no band.
 * Will do gradient with E0240

RFP Device cultures

 * RBS tester had one colony; others had no growth.
 * made overnight culture to look at tomorrow

Gradient PCR

 * Running gel with 2 20-lane rows:
 * 1) E0240 _ L _ _ 1 2 3 ... _ L _ _
 * 2) I2055  _ _ L _ 1 2 3 ... _ _ L _