User:Anthony Salvagno/Notebook/Research/2009/12/01/Unzipping Construct for Doug Smith: Ligation

Today I will be doing the final stage of the unzipping construct for DS, but I will be showing Brian how to do it, which is why it has been so drawn out. We didn't get nearly as much as I did last time, and everything about this process has been a little off. It doesn't matter. Hopefully I get something. Anyways here is the plan for this afternoon:

Recipe

 * 20' -
 * 40' -
 * 60' -
 * 80' -