Smolke:Protocols/Gel extraction

Comb size

 * Single wells from the smallest comb (15 wells per row) can hold up to 20 ul
 * Single wells from un-taped medium comb (8 wells per row) can hold up to 50 ul

Agarose density

 * Above 3 kb: 0.8%
 * 1-3 kb: 1%
 * 250-1000 bp: 1.5%
 * Below 250 bp: 2%


 * 75V for 45 min, or 85V for 35 min
 * Minimizing the amount of agarose in the cutout band seems to improve yields