Huang: DH5alpha transformation

Overview
This protocol allows competent cell transformation.

Materials

 * DH5alpha competent cells
 * LB
 * LB + antibiotic plates
 * Plasmid DNA or DNA to transform the cells with

Procedure

 * 1. Set tubes of frozen cells on ice and let thaw very slowly
 * 2. Add 100ul thawed cells to new eppendorf
 * 3. Add 1-2ul DNA to cells
 * 4. Incubate on ice for 20 min
 * 5. Heat shock in 42C water bath for one min (make sure water bath is at exactly 42C)
 * 6. Incubate on ice 2 min
 * 7. Add 900ul growth medium (LB) and shake 1 hour at 37C
 * 8. Plate about 200ul and grow overnight

Contact

 * Carolina Tropini