User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/15

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Stop stimulation: Protein and RNA/Protein isolation (2)
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * All plates were washed twice with cold PBS
 * 12 plates (6 and 6) were lysed with RIPA as described 24Feb2010 for protein isolation
 * 2 platse (1 and 1) were lysed as described 8June2010 for RNA (and protein) isolation
 * RNA concentrations, see attachment
 * For protein isolation from the phenol/chloroform fraction the pellet obtained was mechanically disrupted using a pipette point
 * AT 22.00 ~ 22.30 cells of D9 P29 were put to S0, cells were less confluent (~90%) but this is believed to better then previous where cells let go of surface during washing and appeared to be dying.

Attachment
   [[Media:15062010_NanodropRNAconcentration.pdf|Nanodrop of RNA concentrations]]


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