User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/05/25

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χρόνος πέρασμα May 25th 2011

 * Today I checked the petri dishes that were left incubating. They all were contaminated. Miguel told us what problems could have been the culprits. Anyway, Fabricio López and I will start it over again.


 * I let incubating at 37° the bacteria DH5α carrying the plasmid pBBRMCS5 in two test tubes with 5ml of liquid LB medium. 1 test tube contains only gentamicin, without bacteria, as a negative control. All test tubes were added with 5µl of gentamicin.


 * I used the thermocycler to amplify the amplification product Pablo García did (tube #2).


 * Tube C is a negative control.


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