User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/31

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Nuclear Gene Adenita Culture 454 Amplification
Culture 53, 55, 56, 58-66

1. Adenita cultures 5-15 individuals. 2. Spin for 1 min at 12-14,000 rpm. Remove supernatant leaving 20-30ul, careful not to disturb pellet. 3. Add 100ul 20% chelex (Or add chelex until ~1/2 tube full of beads). Vortex, then quick spin. 4. Boil for 10 minutes. 5. Before use, vortex, then spin at 13000 rpm for 3 minutes. Use supernatant right above beads.

Load 2.0ul



Nuclear Gene Adenita Culture 454 Amplification
Culture 67, 68, 71-76, 11, 21, 27, 28

1. Adenita cultures 5-15 individuals. 2. Spin for 1 min at 12-14,000 rpm. Remove supernatant leaving 20-30ul, careful not to disturb pellet. 3. Add 100ul 20% chelex (Or add chelex until ~1/2 tube full of beads). Vortex, then quick spin. 4. Boil for 10 minutes. 5. Before use, vortex, then spin at 13000 rpm for 3 minutes. Use supernatant right above beads.

Load 2.0ul