User:Karmella Haynes/Notebook/BioBrick cloning/2011/06/06

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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06/06/11

 * &#x2713; Assemblies: full miR sensors w/ different promoters/ binding site copy numbers
 * &#x2713; DH5α Turbo chemically competent cell prep: streak LB (plain) plates with left-over cells w/o DNA (from transformations)

Assemblies
 * 1) KAH186/196: KAH186/(E/S)/2025 &#x2713; + (1) KAH196/(E/X)/7806
 * 2) KAH184/196: KAH184/(E/S)/2191 &#x2713; + "
 * 3) KAH187/196: KAH187/(E/S)/2269 &#x2713; + "
 * 4) KAH188/196: KAH188/(E/S)/2124 &#x2713; + "
 * 5) KAH189/196: KAH189/(E/S)/2296 &#x2713; + "
 * 6) KAH190/196: KAH190/(E/S)/2368 &#x2713; + "
 * 7) KAH191/196: KAH191/(E/S)/2223 &#x2713; + "
 * 8) KAH183/196: KAH183/(E/S)/2383 &#x2713; + "
 * 9) KAH192/196: KAH192/(E/S)/2461 &#x2713; + "
 * 10) KAH186/177: KAH186/(E/S)/2025 &#x2713; + KAH177/(E/X)/7978 &#x2713;
 * 11) KAH187/177: KAH187/(E/S)/2269 &#x2713; + "
 * 12) KAH188/177: KAH188/(E/S)/2124 &#x2713; + "
 * 13) KAH189/177: KAH189/(E/S)/2296 &#x2713; + "
 * 14) KAH190/177: KAH190/(E/S)/2368 &#x2713; + "
 * 15) KAH191/177: KAH191/(E/S)/2223 &#x2713; + "
 * 16) KAH192/177: KAH192/(E/S)/2461 &#x2713; + "
 * 17) KAH186/198: KAH186/(E/S)/2025 &#x2713; + (2) KAH198/(E/X)/8050
 * 18) KAH184/198: KAH184/(E/S)/2191 &#x2713; + "
 * 19) KAH187/198: KAH187/(E/S)/2269 &#x2713; + "
 * 20) KAH188/198: KAH188/(E/S)/2124 &#x2713; + "
 * 21) KAH189/198: KAH189/(E/S)/2296 &#x2713; + "
 * 22) KAH190/198: KAH190/(E/S)/2368 &#x2713; + "
 * 23) KAH191/198: KAH191/(E/S)/2223 &#x2713; + "
 * 24) KAH183/198: KAH183/(E/S)/2383 &#x2713; + "
 * 25) KAH192/198: KAH192/(E/S)/2461 &#x2713; + "

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

--> Make master mix w/ lign. buffer, vector DNA; add insert DNA to individual tubes; add ligase to master mix and quickly aliquot --> Add rxn. to 25 μL DH5α Turbo cells


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