IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/29/Shan Shen

1:30 Recycle the fragments of single digestion of T7 promoter. Electrophoresis to recycle the fragments of 1-12M and 1-12O The order and the amount of the samples: marker 10μL, plasmids of 1-12M 10μL; digestion products of 1-12M, plasmids of 1-12O, digestion products of 1-12O. Results: 2:00 Single digestion of the T7 promoter fragments

2:20 Start to digest. 14:00 Electrophoresis to test the efficiency of double digestion The order and the amount of the samples: marker 10μL, plasmids of T7 promoter 10μL, digestion products of T7 promoter 10μL. Results: 15:00 Recycle the fragments of T7 promoter, both double digestion and single digestion. 15:30 Add CIAP into another system of T7 promoter. 16:00 Electrophoresis to recycle the fragments of 1-12M and 1-12O The order and the amount of the samples: marker 5μL, plasmids of T7 promoter 10μL, digestion products of T7 promoter 10μL. 16:50 Recycle the double digestion products of T7 promoter. 20:30 Ligation: T7 promoter + GFP System of Ligation 21:00 Start to ligase