User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/16

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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CCA3 Qiagen Extraction
~100-200 individuals Used 1mM NaCl to make rotifers curl, then moved to extraction tube

Followed standard Qiagen tissue extraction protocol

Nanodrop DNA Tube (1) 6.9 ng/ul Tube (2) 3.1 ng/ul

R Takara Test Nuclear Genes
Load 2.0ul sample



R Takara Test and HS Takara Test Nuclear Genes
Load 2.0ul sample



PCR test (TopTaq)
load 2.5ul of sample



Alien Genes New Primer Screen
load 2.5ul of sample



Gene-rich region of CCA3 -- take 2

 * this time going to try the same thing but with higher DNA concentration

PCR conditions

 * Primers:
 * 94C 1 min, (98C, 10 sec;65C 10 min)x30, 72C 5 min