IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-11-14

Goals This Week

 * Fold design 5. Will fold without lids - no aptamers, inside aptamers, outside aptamers. Aptamers here simply refer the the dna ligands we plan to cut with restriction enzymes.
 * Fold design 5 lids
 * Fold with biotin for Tiff's protocol
 * PEG fractionation
 * Prepare to repeat Tiff's experiments
 * GUI development

Mixed PreWorking Stocks
c5.0.1 - core barrel oligos Total = 172
 * box_v5_A
 * box_v5_B rows A-F
 * box_v5_B row G (1-4)

c5.0.4 - barrel oligos at outside aptamers (without aptamers) Total = 5
 * box_v5_E row A (5-9)

c5.0.5 - barrel oligos at inside aptamers (without aptamers) Total = 5
 * box_v5_E row A (10-12), row B (1-2)

c5.0.4L - barrel oligos at outside aptamers (with ligand) Total = 10
 * box_v5_F row F (1-10)

c5.0.5L - barrel oligos at inside aptamers (with ligand) Total = 12
 * box_v5_F row F (11-12), row G (1 - 10)

c5.0.6 - barrel oligos at latches (no latches) Total = 3
 * box_v5_E row B (3-5)

c5.0.7 - barrel oligos at latches (no latches)
 * box_v5_E row B (6-8)

Mixed Working Stocks
c5.0.A - barrel, lidless
 * c5.0.1 - 172 ul
 * c5.0.4 - 5 ul
 * c5.0.5 - 5 ul
 * c5.0.6 - 3 ul
 * c5.0.7 - 3 ul
 * dH20 - 12 ul

c5.0.C - barrel, lidless, inside ligand
 * c5.0.1 - 172 ul
 * c5.0.4 - 5 ul
 * c5.0.5L - 12 ul
 * c5.0.6 - 3 ul
 * c5.0.7 - 3 ul
 * dH20 - 5 ul

c5.0.D - barrel, lidless, outside ligand
 * c5.0.1 - 172 ul
 * c5.0.4L - 12 ul
 * c5.0.5 - 5 ul
 * c5.0.6 - 3 ul
 * c5.0.7 - 3 ul
 * dH20 - 5 ul