Koch Lab:Protocols/GTP

May 2009--Stock GTP from Cytoskeleton
Steve Koch 01:46, 16 June 2009 (EDT): When we made this, we made a mistake of resuspending in water, not MgCl2. At this point, I don't remember the details of what tech support said. But this is what we did:


 * (See: User:Anthony Salvagno/Notebook/Research/2009/05/12/Microtubule creation)


 * Have 100 mM GTP (sodium) in water (or buffer?/whatever is in the powder). Have 1M MgCl2.  To make it equimolar of MgCl2.
 * 5 ul MgCl2
 * 50 ul resuspended GTP in water
 * 45 ul of cold H2O

Final concentration is 50 mM GTP

The rest of the non-magnesium GTP
We had 50 ul remaining of the GTP resuspended with water. This is still in the cytoskeleton tube.