User:Maiko Furubayashi/DNA construct

プロモータ

 * parts registry: promoters

pLtetO-1, pLlacO-1, plac/ara-1

 * Ref
 * Lutz 1997 NAR

pBAD/araC

 * nativeのものはall-or-none expression.
 * araE transporterをconstitutiveに発現させればhomogeneous expression. arabinoseの膜透過性が低いため.
 * Placでも同様にlactoseの膜透過性が低いためall-or-none型になるが，IPTGは膜透過性が高くhomogeneous Khlebnikov A 2002 Biotechnol Prog
 * Refs
 * Khlebnikov A 2000 J Bacteriol
 * Khlebnikov A 2001 Microbiol
 * Khlebnikov A 2002 J Ind Microbiol Biotechnol

modified pT5/lac

 * DNA2.0由来，pT5とlacOの融合プロモータ. IP free.
 * lacOへのmutationで抑制が強くしてある. "This operator pair ensures significantly tighter repression than regular lac operators."
 * Ref
 * DNA2.0 pJexpress vectors

RBS

 * Salis HM 2009 Nat Biotechnol
 * RBS Calculator from Voigt lab
 * RBS calculator v1.1 from Salis Lab


 * まとめたいものリスト
 * library creation
 * terminator
 * codon optimize法