User:Kristina M Holton/Ovarian Notebook 2007 9 13

Ambion Recoverall Kit: G02, G03, Drapkin

10-10 micron samples, split into 2 tubes (50 microns each)
 * Deparaffinization Step 2: still appreciable parafin
 * G03: No visible tumor sample after xylene spin-down
 * Deparafiinization Step 4-1: Add EtOH, vortex = cloudy, poorly pelleted after 2 minute spin-down
 * Deparaffinzation Step 4-2: Spin down 2.5 mins, slightly better

T=3hrs: samples did not fully clarify

Nucleic Acid Isolation Step 2: Only partial clarification after add Isolation Buffer, large amount tissue not digested