Jhdavis::4x reducing SDS-PAGE loading buffer

4X SDS-PAGE sample loading buffer From OpenWetWare

10 ml					1X

2.0 ml 1M Tris-HCl, pH 6.8		50 mM

0.8 g SDS				2%

4.0 ml 100% glycerol			10%

0.4 ml 14.7 M -mercaptoethanol	1%

1.0 ml 0.5 M EDTA			12.5 mM

8.0 mg bromophenol blue		0.02%

2.6 ml H2O

Use at 1X for loading samples onto SDS-polyacrylamide gels.