IGEM:PennState/Labbook/NoahJohnson/2007-7-4

July 4, 2007
 * Poured gel
 * Ligation (Started at 12:50):
 * (+) 20uL 1741015-PSB1A2
 * 2uL T4 Ligase buffer
 * 4ul Vector (P1010 in PSB1A2+S+E)
 * 12uL Insert (I741015+S+E)
 * 1uL T4 DNA Ligase
 * 1uL H2O
 * (-) 20uL PSB1A2
 * 2uL T4 Ligase buffer
 * 4uL Vector
 * 1uL T4 DNA Ligase
 * 13uL H2O


 * Ran Transformation
 * Plated on LB Amp

9:30-3:30