User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/03

Single stranded probe preparation



 * Second band should be single-stranded DNA. I hope. Frozen @ -20.
 * This should give us an approx 1 μM solution of single-stranded probe, and can be used for hybridization without further purification.

Trying to get some long DNA segments from P.rapida

 * got some large molecular weight DNA from Priya
 * Calculations:
 * Melted gel at 65C, then equilibrated at 42C for 10 min.
 * Added 7.3 ul AgarACE at 7pm