SBB10Ntbk-JennaKelleher1

Project Information
29071-O99 rep Protein, CA42 rep Gene, Pre-Pro sequence

Construction Files
Jenna Kelleher 099 Rep Protein (sbb29) Jenna Kelleher CA42 Rep Gene (sbb28) Jenna Kelleher Pre-pro Sequence (sbb42)

Useful Protocols
For SBB28 and SBB29: Cloning by PCR Regular Zymo Cleanup For SBB42: Eco-Bam Transfer Small-Frag Zymo Cleanup For All 3: EcoRI/BamHI Digest of PCR Products Zymo Gel Purification Ligation of EcoRI/BamHI digests Transformation by heat-shock Picking of colonies Macherey-Nagel Miniprep EcoRI/BamHI Digest of PCR Products Analytical digests (Mapping)