Sentelab:Notebook/gene synthesis/2010/09/17

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] GENE SYNTHESIS
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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gene synthesis by using 2-step pcr

 * 1st PCR mixture
 * 1) 42.75 uL ddH2O7
 * 2) 1ul gfp 1-11 oligos (pooled) or gfp 12-22 pooled oligos
 * 3) 1.25 ul dNTP mix(10mM)
 * 4) 5 ul 10X pfu polymerase buffer with MgSO4
 * 5) 1 ul Pfu turbo hotstart polymerase


 * PCR conditions
 * 1) 95C for 2 min
 * 2) 95C for 30 sec
 * 3) 50C for 30 sec
 * 4) 72C for 1 min
 * 5) 72C for 2 min
 * 6) 4C unlimited time


 * results




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