User:Tara K. Luckau/Notebook/Team ConGen/2010/09/27

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 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
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 * style="background-color: #9DB68C" align="center"|  |Main project page


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Ordered Primers

 * Ordered first set of primers
 * Scun2 and Scun22 (highly variable simple motifs)
 * Lance et al (2009) Conservation Genetics Resources, Sceloporus undulatus
 * Forward and reverse as given in publication, also reverse with pigtail (GTTTCTT) at 5' end, also forward with M13F(-21) tag (TGTAAAACGACGGCCAGT)
 * [[Image:Luckau_Primer20100927.jpg|500 px]]
 * Try all four combinations
 * 1) F - R
 * 2) F - R pigtail
 * 3) F M13 - R
 * 4) F M13 - R pigtail
 * If they don't hinder efforts or change optimization decisions, then may make optimization of multiplexes cheaper!


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