Davidson:Presentation Outline

Things to keep in mind...
 * 1) We should have each team work as separate units to facilitate team spirit and ability to practice. However, we will share slides for continuity of presentations.
 * 2) We will probably have to let go of who did what in order to have two smooth presentations. We can have a color-coded schematic slide early on that shows where different parts were built but this does not affect who presents what. In fact, this chart will help people see how well the two schools worked in parallel.
 * 3) We need to have talks ready and two posters.

This outline is the one Karmella started and was edited by the Western team.


 * INTRODUCTION (MO Western)
 * Our goals
 * Solve a mathematical problem using bacteria
 * Integrate math and biology students holistically
 * Work in tandem with two campuses to test parallel processing of PUIs for iGEM
 * Design a device that would be more than just a widget
 * Have a lot of fun learning
 * Define the math problem: burnt pancake problem
 * The Hin recombinase system in Salmonella – flagellar antigen variation
 * Turning the Hin recombinase system into a BioBricks system - Hix, Hin, RE
 * Schematic design of 1 & 2 pancake stacks
 * Computing with E. coli - detecting orientation of DNA pancakes using the antibiotic resistance screen (two-pancake system, animation by Karmella)
 * Design of 4 pancake constructs
 * Mathematics behind solution and possible number of flips per n pancakes
 * Biological equivalence problem - will be solved using backwards RFP (described later)


 * METHODS (Western)
 * Building new parts - Hix, Hin, antibiotic genes, backwards parts
 * how we assembled small DNA segments too big for oligos (Lance’s web site)
 * how we generated backwards biobrick parts with PCR (switch-a-roo)
 * List of Basic Parts – Hin (+/- LVA), Hix, RE, etc.; end with this; highlight who did what
 * Transition to Data

--Kahaynes 15:49, 6 October 2006 (EDT) (below)