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Circuit design. When our redesigned rbp binds vanillin, the complex will bind trg that will cause phosphorylation of OmpR at the EnvZ domain (we use a chimeric trg-EnvZ fusion), which will regulate several designed promoters. We use an AND promoter. We don't expect (we hope) that for high-copy number plasmids the use of the promiscuous CRP will cause much trouble.