Prince:Notebook/Mouse Brain Phosphoproteomics

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Project Description

 * Determine differential phosphorylation in brain proteins of NTG stimulated/unstimulated CK1-delta mutant and wt mice.

Project Members

 * Aaron Sahim
 * Aman Makaju
 * John Prince
 * Emily Bates

A collaboration between the Bates and Prince Labs.

Current Objectives

 * Determine best method for mouse brain harvest
 * Determine best TiO2 column to buy (or other method?)
 * Establish/understand what work has been done on mouse brain proteomics/phosphoproteomics before.
 * how many proteins? how many phosphosite/phosphopeptides/phosphoproteins?
 * was it quantitative?
 * How successful are trypsin digest with O18 for quantitation?
 * What software has been used to quantify O18 differences?

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