Streptomycin

Mode of Action
Inhibits protein synthesis; binds to 30S ribosomal subunit.

The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common.

Usage Notes

 * 1) 100 mg/mL stock solution in H2O. It is barely soluble in ethanol so you must dissolve it in H2O.
 * 2) Filter-sterilize with 0.22 &mu;m filter.
 * 3) Add 1 mL per liter of culture.