Klapperich Lab:Notebook/Lab Meeting Notes/2008/10/07

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7 October 2008 Lab Meeting Agenda
† Sonali's research report today.

† Announcements † Flu R01 † SEPSIS Project - CIMIT Bird Flu †RNA project † COBRA † Valve Array/Valve building (FJ) † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR '''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ''' † RCA † Senior project † F31: Cochlea † Silica Optimization (Lambda):
 * A slow migration to 709 can now begin.
 * JZ and JD out next week at mTAS.
 * More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly? YES. Cathie will amend.
 * Plaque assay status. Redo . Too dilute.
 * Cathie STILL STILL has to submit the progress report to INSPIR.
 * Sample (raw pellets and film) from Ticona COC ordered.
 * B sub in blood is done. Lets talk about comepleting the gram positive plots so they "match."
 * SEM image analysis of pore size. Progress?
 * Run E. coli RT-PCR using primers for a genomic target.
 * Report DNA amounts along with the RT-PCR data for the Sepsis paper.
 * Tube meeting took place last week. More to come.
 * Jeff Braman will visit in early Dec.
 * SM and AC did the first experiments this week. RNA obtained. Looks clean.
 * Do dilutions. Send to Bioanalyzer.
 * Mark finish control circuit
 * Fluorescence microscopy available from 720. Try beads experiment. Update?
 * Cutter plotter arrived.
 * Jane, Cathie, Larry, Ranjith will meet soon to discuss scale up of sol gel in chip process.
 * Discuss dispensing from evap chip in a real world way.
 * Current design gives E.coli signal, clotting is still a problem
 * Change of design: funnel-shaped wells, smooth hole, make samples in pipette tips?
 * Waiting for fixture still from Melissa. Hopefully this week.
 * Update: Monday 6 Oct Haven't heard anything from Shane yet. Attempting to contact again, so far without success.
 * Update?
 * Alex working on more toluene bonding issues.
 * Try Ticona material. (HM, JD, AG)
 * Still Still Still troubleshooting ??
 * Mold still not made. Mask potentially Wed AM. When mask is made.
 * Do preliminary E. coli experiment soon. Sonali will train.
 * Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
 * STAR-CD Renewal - January ($2K)
 * Physical PCR areas defined in 709 (check with Sonali).
 * QQ continued with Taqman assays.
 * QQ will run it on a gel to check.
 * Run Gels for water Wash steps - show me and Sonali
 * Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week.
 * MinCheol is making Four simple PCR models to optimize temperature distribution along to the channel. Will do another model with the insulation on the bottom.
 * MinCheol is simulating DNA's Residence Time for simple PCR models. The DNA particles need to be much smaller. Also, consult the Lab on Chip paper that JD mentioned.
 * Have a PCR only meeting sometime next week.
 * Qingqing has to do experiments with MinCheol's designs.
 * Cathie Still Still Working on Whitepaper almost done!
 * Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources.
 * Haven't seen Teddy yet. What is up with him?
 * ARO Abstract submitted.
 * Data summary focusing on the fundamental biological questions for next week.
 * Ongoing. More concentrations. Forged chips.
 * Pressure testing on these channels when they are flowing.




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