User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/04/19

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Heat shock transformation:

 * Take 50ul of competent cells from Miguel’s lab frozen at -70ºC, out of a 100ul eppendorf into a new one (sterilized), then I add 5ul of the DNA (luciferase from Photinus pyralis BBa_I712019) and put into ice for 20 minutes.


 * After that give it a heat shock at 42 ºC for one minute, then put it in ice for another 5 minutes.


 * Now grow it in 1ml of liquid LB medium with no antibiotic and shaking for one hour.


 * Then centrifuge for 1 minute at 13 rpm and keep the pellet with 100ul, now plate those 100ul in a petri box with kanamycin using the triangle, also plate a control with the sole medium and put them in the incubator at 37ºC overnight.


 * }