Morison: Recipes for solutions

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50xTAE buffer
Preparation: For 1 litre of 50x TAE buffer use:

242 g Tris base (2-amino-2-hydroxymethyl-propane-1,3-diol) (= 2 mole)

57.1 ml glacial acetic acid (= 100% acetic acid) (57.19 ml = 1 mole)

100 ml 0.5M Na2 EDTA (pH 8.0)

H2O up to 1000 ml

10xTAE buffer (Marie's recipe)
Preparation: For 2 litres

96.8 g Tris base

8.2 g sodium acetate anhydrous

7.6 g Na2 EDTA (pH 8.0)<br / Adjust the pH to 7.9 with glacial acetic acid (ca. 40ml)

0.5M Na2 EDTA (pH8.0)
Preparation: For 1 litre

add 186.1 g of disodium ethylenediaminetetraacetate.2H2O to 800 ml of H2O. Stir vigorously.

Adjust the pH to 8.0 with NaOH (ca. 20 g of NaOH). Sterilize by autoclaving.

Hint: The disodium salt of EDTA will not go into solution until the pH of the solution is adjusted to ca. 8.0 by the addition of NaOH.

5xTBE
Preparation: For 1 litre of 5X stock solution

53 g of Tris base

27.5 g of boric acid

20 ml of 0.5 M EDTA (pH 8.0)

TBE should be diluted to 0.5X prior to use in electrophoresis.