Matt Gethers/CRI, Thailand/Labwork/Gels/Week of 6.29.08

=6.29.08=

100 volts, 30 minutes, 1% gel, 20 minutes staining. This was a gradient PCR to determine the optimum annealing temperature. All lanes showed product (50 to 65 degrees).

=6.30.08=

Gel #1

100 Volts, 30 minutes, 1% gel, stained for 20 minutes. The positive control worked beautifully and it looks like colony 3 turned up a product at the correct length.

Gel #2

100 Volts, 30 minutes, 1% gel, stained for 20 minutes. Nothing showed up at all, including the positive control.

=7.2.08=

100 Volts, 30 minutes, 1% gel, stained for 20 minutes. Every lane had some product, but there were usually multiple bands (some at ~600, then 1500, and one higher, I think). Of all the colony PCR lanes, 1 and 2 had the brightest bands at ~600.

=7.4.08=

100 volts, 30 minutes, 1% agarose. Stained in EtBr for 15 minutes. Excised band at ~4.2 Kb.