User:Karmella Haynes/Notebook/Polycomb project/2010/06/24

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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06/24/10

 * &#x2713; Cell culture: expand Pc-ATF lines 129-4(1/23/10), 131-9 (10 cm plates)
 * &#x2713; Senescence assay: KAH126-1, 126-3, 132-8, 154-2 -/+ dox and FTRx +rotenone
 * &#x2713; Transformation (bact.): quick and dirty transformation for KAH96, 142, 146, 147 into C2925 (dam-/dcm-); morning
 * &#x2713; Cultures: pick one colony each, grow 5 mL culture o/n
 * &#x2713; Transfection prep: seed FTRx plate for H3me sensor stables (try DNA -met plasmids); ~200k cells/ well, 6-well plate

Senescence assay > Samples (plate ~200k cells/ well, 6-well plates, ~3.5 mL medium/ well):
 * 1) KAH126-1
 * 2) KAH126-1 +1 μg/mL dox
 * 3) KAH126-3
 * 4) KAH126-3  +1 μg/mL dox
 * 5) KAH132-8
 * 6) KAH132-8  +1 μg/mL dox
 * 7) KAH154-2
 * 8) KAH154-2  +1 μg/mL dox
 * 9) FTRx + 0 rotenone (7.0 μL DMSO)
 * 10) FTRx + 0.1 μg/mL rotenone (1.75 μL/ 5.25 μL DMSO)
 * 11) FTRx + 0.2 μg/mL rotenone (3.5 μL/ 3.5 μL DMSO)
 * 12) FTRx + 0.4 μg/mL rotenone (7.0 μL/ 0 μL DMSO)


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