User:Nkuldell/other rewrites

To replace 704-1055 with URA3 marker from pRS406

 * SPT6 from 704-744: 5'- CTGGTCTATCGTCGGATAAGATTGACGAGATGTATGACAT
 * fwd URA3: 5' AGATTGTACTGAGAGTGCAC
 * SPT6 from 1015-1055: 5' ATGTCATCGGAGGATCAAGAATTAGAAAGAAACTGGATAG
 * rev complement SPT6 (from 1055-1015): 5'- 5'- CTA TCC AGT TTC TTT CTA ATT CTT GAT CCT CCG ATG ACA T
 * rev URA3: 5' CTGTGCGGTATTTCACACCG

spt6:URA3_704_1055_fwd
5' CTGGTCTATCGTCGGATAAGATTGACGAGATGTATGACATAGATTGTACTGAGAGTGCAC

spt6:URA3_704_1055_rev
5' CTGTGCGGTATTTCACACCGCTA TCC AGT TTC TTT CTA ATT CTT GAT CCT CCG ATG ACA T

ideas

 * stepwise move centromere from one end of chromosome to other
 * progressively larger deletions from telomere to first essential gene on each chromosome
 * clustering highly expressed genes on one arm, poorly expressed genes on other
 * clustering related genes into "operon"
 * multiple copies of yeast mating locus
 * replace S. cerevisiae genes for single complex (e.g. SAGA) with homologs from other yeast starting with pombe
 * in vivo reporter for transciption elongation: functional RNA?
 * randomize sigma 4.2 region of TFG2 and screen library for rifS and FLO:HIS mutants
 * check Drew's T7.1 for revertants that increase plaque size back to normal.
 * biobrick assembly for yeast parts (look at Pam's yeast parts first!)...ideally would use homologous recomb vs restriction sites