User:Tk/Notebook/MF/2008/09/14

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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In-Fusion protein identification

 * Re-run In-Fusion gel
 * 10% Bis-tris gel
 * 150 V, 1 hr 20 min
 * prerun 30 min
 * dissolve In-Fusion mix in 13 ul water
 * Add to serial dilution mix of:
 * 2 ul beta-mercaptoethanol
 * 5 ul LDS sample buffer
 * 3 additional mixes of BME + LDS + 13 ul water
 * 2x serial dilutions into the LDS mix
 * incubate 15 min at 70C
 * run 10 ul SeeBlue plus 2 marker lanes
 * run 20 ul of each dilution
 * wash 2x with microwaved DI water 10 min
 * Add Simply Blue stain covering gel, incubate overnight
 * Wash 2x with hot DI water + kimwipes
 * Image with gel imager in water
 * store in plastic bag




 * There appears to be two proteins present
 * One is about 68 kD
 * A second, about 8x less in mass, appears to be about 155 kD


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