IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/07

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Overnight Cultures

 * Prepared 4 overnight cultures in order to make competent cells of BW27783 and DH5α and to miniprep the chlor and amp construction plasmid
 * Innoculated 5mL of LB broth with 09/05/13 BW27783 cells
 * Innoculatd 5mL of LB broth with 09/05/13 DH5α cells
 * Innoculated 5mL of LB-amp broth from a plate of DB3.1 cells containing the Amp construction plasmid
 * Innoculated 5mL of LB-chlor broth from a plate of DB3.1 cells containing the chlor construction plasmid
 * All cultures were grown overnight in a shaking incubator at 32°C


 * }