IGEM:metu/2009/Notebook/wound dressing/2009/08/28

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28.08.2009
1. Electrophoresis order; 1)leader / 6M / 8M / 6 / 6 / leader

2. We made digestions.The following amounts are added to microtubes;

6M,          water       18.4 µl Buffer      3 µl Pst1        3 µl DNA         5.6 µl     =30 µl

1st part                  2nd part                 control 8M/D,        water       17.9 µl                      8 µl                   34 µl Buffer      3 µl                        4 µl                    4 µl Pst1        3 µl               Xba1     4 µl                    0 DNA         6.1 µl   =30 µl            24  µl      =40 µl       2 µl        =40 µl

3. Electrophoresis order; 1)empty/empty/8MD/8M control/leader

4. We couldn't digest.