User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/30

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Trypsin/EDTA dilution, splitting cells for ELISA
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * EDTA solution
 * 2.5 mg EDTA in 10 mL PBS (0.05% EDTA)
 * 5.5 mg EDTA in 22 mL PBS
 * Dilute trypsin 5x
 * Cells were split to P28, 2x 24 wells for ELISA and 4x 10cm dishes for continuation
 * Immunofluorescence was looked at under microscope, it seemed quite fine
 * Cy3 signal of RII units was a bit faint, look into antibody or use different label
 * Use control were only secondary antibody is used!!


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