User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/21

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Reaction
1.67 μL pMAL-c2X Digest (50 ng) 1.33 μL PCR Product Digest (37 ng) 7 μL MilliQ 10 μL Quick Ligase Buffer 1 μL Quick Ligase

Conditions

 * Incubate at room temperature for 1 min

Control

 * 1 sample replaces PCR Product Digest with MilliQ water.

Transformation

 * XL1-Blue Competent Cells
 * Plated onto 4 Amp plates (3 sample, 1 control)


 * }