User:Fermenter User/Notebook/BROM B11

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM B11

Day 0 (Nov 10 Tue):
           11/10/2009 12:30 Assemble bioreactor             11/10/2009 12:30 Bring media to fermenter room            11/10/2009 13:00 Add 5 ml of (Sterilized) Antifoam 11/10/2009 13:25 Start Sterilization (Liq 45 min) 11/10/2009 15:18 Cooling of bioreactor (Temp setup) 11/10/2009 15:19 Start Fermentation Software for Fermenter #6 Record 0:00 11/10/2009 15:20 Purge with 1.0 L/min O2                          11/10/2009 15:22 Connect Acid (500 ml) and Base (400 ml), prime pumps 11/10/2009 15:22 Start pH control 11/10/2009 15:27 Calibrate dO2 probe with 350 rpm, Air purge for 30 min 11/10/2009 16:00 Set dO2

---                11/10/2009 24:00 Inoculation: (240 ml) Fermentation 0:00

Day 2 (Nov 12 Thu):
            11/12/2009 (12:00) Sampling Fermentation 36:00 F6: Cell weight (112 mg/ml), Activity ( 91, x100), Activity ( 55, x1000)             11/12/2009 (18:00) Sampling Fermentation 42:00 F6: Cell weight (110mg/ml), Activity ( 88, x100), Activity ( 51, x1000)             11/12/2009 (18:00) Fermentation 42:00 Harvest

Day 3 (Nov 13 Fri):
11/13/2009 (09:00) Fermentation ??:00 F6: Acid (400 ml) Base (190 ml)


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