User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/10

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Thursday 10th June 2010
I re-checked the double restriction on an agarose gel, but it came out the same:



So I re-did the double restriction with PSTI and EcoRI as follows:

Reactive.....1x [ul]

DNA...........5

BSAa..........1

Buffer2......2

H2O..........10

EcoRI........1

PSTI..........1

Total.........20


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