Lactobacillus culture

Overview
General overview and guidelines on how to grow up a culture of Lactobacillus

Materials

 * MRS broth (difco)
 * MRS agar (difco)
 * anaerobic conditions

Procedure

 * 1) Follow instructions to make MRS plates and media, autoclave to sterilize
 * 2) Streak Lactobacillus on MRS plate or inoculate into test tube of MRS media
 * 3) Incubate at 37C under anaerobic conditions
 * 4) If from new source of Lactobacillus (ex. dehydrated bacteria from ATCC) allow up to 3 days to grow. Otherwise, allow 12-24 hours to grow

Contact

 * Derek Ju (derekju [at] mit.edu)

or instead, discuss this protocol.

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