IGEM:MIT/2005/Monday 8/8 Meeting Agenda and Minutes

General Updates

 * We are currently working out what everyone's schedule will be like for the rest of the summer and over the semester
 * We are also transitioning to a new lab bench in Endy lab

SubTeam Updates
Format: What do you hope to achieve this week? What do you need help with? (Also update what you expect your schedule to be under the "Schedule" section.)

Input: Maxine

 * Will look over notes to see where variability may have occurred
 * Check fluorescence of oligos
 * Still in planning stage of what direction we should take
 * Meanwhile will be helping with FecA system

Receiver Head-Unit: Jenny
PCR start codon Pro.RBS.scFv for testing by itself.

Receiver/Transmitter 1 - ToxR : Wiki-Will

 * ctx::gfp made
 * verify after meeting.
 * waiting for ToxR - Alternative Mechanisms for ToxR retrieval?
 * getting my wits about lab, devising plan to deal with numerous fusions (scFv i,ii,and iii, malE, and phoA) and testing them.
 * Give Me ToxR

Receiver/Transmitter 2 - FecA : Annie

 * Friday & Weekend:
 * + Competent DY329 cells
 * + Reorder primers
 * + Ran PCR to make Tetracycline and Chloramphenicol Resistance


 * LAB:
 * + FecA, FecR, PhoA QuikChange -- Work w/ Maxine
 * + Make FecA- and Fur- -- Work w/ Ray
 * + RBS, FecI, FecR -- Work w/ Jenny
 * + FecA promoter BB -- Work w/ Jen

Schedule

 * Maxine
 * Summer: taking the last week before school off (August 31-Sep 7)
 * School year: will take igem for credit (not sure how many hours yet)
 * Attending Jamboree? yes
 * Jenny
 * scFv testing in lab till end of week.
 * Working with Texas team. :D
 * Will
 * Summer: Sister coming this week - missing Friday, maybe part of Monday. Unless ToxR shows up.
 * Parents coming next week - may miss something, probably.
 * considering a week / half week no-work-binge for the tail end of August. Probably depends on if ToxR shows up.
 * School Year: Is 5.12 going to pwn me?
 * Looking at some UROP time (credit/pay undecided). Will we officially/unofficially have full time lab access? Times may not be conventional.
 * will try not to let school get in the way of my education. but I haven't ruled out med. school yet!
 * Attending Jamboree? yes sir, mam.
 * Ray
 * Will leave for Cambridge in October.
 * Attending Jamboree? maybe?
 * Annie
 * ANNIE IS TAKING A FEW DAYS OFF!!
 * Jessica
 * Jen