IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/15/Min Lin

9:00 MiniPrep 1-12M and 1-12O. Digest with EcoRI and XbaI Transformation (pSalSer plasmid from Voigt Lab into Top10)

22:00 Digest 1-12M and 1-12O with EcoRI and SpeI overnight.

Digest T7 promoter with