User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/04/20

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April 20th, 2010
1. Look for reporter plasmid to test YcgE promoter.
 * Chosen: BBa_E0430: EYFP (RBS+LVA-TERM)
 * Standard YFP Output device-LVA tag
 * Location:
 * 2009 Kit Plate 1	Well: 8I	Plasmid: pSB1A2
 * 2008 Source Plate 1000	Well: 5D	Plasmid: pSB1A2
 * 2007 iGEM 2007 Parts Kit Plate 1	Well: 11A	Plasmid: pSB1A2

2. Prepare culture dishes.
 * Heat solid medium 7 min at potency 30 and cool at 50ºC.
 * Add antiobiotic (1ml of medium:1ul antibiotic).
 * Mark dishes in the narrow side ant pass them by the fire.
 * Pour medium with antibiotic into the dishes.
 * Pass the dishes with the medium by the fire to eliminate bubbles.
 * Let the medium set.
 * Close and put parafilm in the dishes.


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