User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/25

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05/25/11

 * &#x2713; Cultures/minipreps/digests: KAH184(12)
 * &#x2713; Assemblies: KAH176fwd/183, 176fwd/184, 177fwd/183, 177fwd/184

Minipreps > Check with E/P digests

Assemblies
 * 1) KAH176fwd/183: (1) KAH183/(E/S)/2383 + (3) KAH176/MV8 /(E/X)/7785
 * 2) KAH176fwd/184: (2) KAH184/(E/S)/2191 + "
 * 3) KAH177fwd/183: 5' part/(a/b)/2383 + (4) KAH177/MV8 /(E/X)/7978
 * 4) KAH177fwd/184: 5' part/(a/b)/2191 + "

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

--> Add 10 μL to 30 μL DH5α-Turbo


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