Griffitts:Gel recipes

Resolving gel (15%) (5 mL)

 * 550 μL dH20
 * 938×2 μL 1 M Tris pH 8.8
 * 25 μL 20% sodium dodecyl sulfate (SDS)
 * 833×3 μL 30% acrylamide (29:1)
 * 50 μL 10% ammonium persulfate (APS)
 * 3 μL tetramethylethylenediamine (TEMED; in fume hood)

Resolving gel (10%) (5 mL)

 * 690×2 μL dH20
 * 938×2 μL 1 M Tris pH 8.8
 * μL 20% sodium dodecyl sulfate (SDS)
 * 833×2 μL 30% acrylamide (29:1)
 * 50 μL 10% ammonium persulfate (APS)
 * 3 μL tetramethylethylenediamine (TEMED; in fume hood)

Stacking gel (4%) (3 mL)

 * 725×3 μL dH20
 * 375 μL 1 M Tris pH 6.8
 * 15 μL 20% 20% sodium dodecyl sulfate (SDS)
 * 400 μL 30% acrylamide (29:1)
 * 30 μL 10% ammonium persulfate (APS)
 * 5 μL tetramethylethylenediamine (TEMED; in fume hood)

Native Resolving gel (10%) (5mL)

 * 900×2 μL dH2O
 * 938×2 μL 1 M Tris pH 8.8
 * 625×2 μL 40% acrylamide (29:1)
 * 50 μL 10% ammonium persulfate (APS)
 * 3 μL tetramethylethylenediamine (TEMED; in fume hood)
 * Overlay with dH2O

Native Stacking gel (4%) (3mL)

 * 760×3 μL dH2O
 * 375 μL 1 M Tris pH 6.8
 * 300 μL 40% acrylamide (29:1)
 * 30 μL 10% ammonium persulfate (APS)
 * 5 μL tetramethylethylenediamine (TEMED; in fume hood)