Klapperich Lab:Notebook/Lab Meeting Notes/2009/03/19

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19 March 2009 Lab UPDATE - NO MEETING
Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look STATUS?
 * Co. says that it works fine. Transducer up to 1000 psi. We are getting it back.

† Announcements † Flu R01 on-chip PCR is for M1 gene,93bp, same as sonali's assay. † SEPSIS Project †RNA project † COBRA - nanopatterned COP provides access to repeatable morphology - Engineered COP substrate? UPDATE? - Do Rhodamine. -Need a size specific positive control. PS beads? charged? † Valve Array/Valve building - done.
 * MicroTAS abstracts. I NEED THESE BY 3/18 so I can edit them!!!
 * I need an update on where we are in the RT PCR process with the samples (off-chip). What PCR are we doing? The M1 gene, or something else.
 * BUMC _ John Connor - Cathie STILL working on IBC amendment. Two transportation steps
 * Submitted to LOC.
 * Need to get Jurkat RNA to Jeff.
 * Integration test from here forward.
 * Substrates (JZ)
 * three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD)
 * Colloid experiment.
 * ordered
 * 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
 * co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles

† Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR † RCA/HDA † Senior project † F31: Cochlea
 * Paper submitted to LOC
 * Cathie in charge of Bionterfaces Paper1 (trapping?) ? Let me know.
 * Jason promised me add his change part soon.
 * I will change Fig. 2a-b (10 um channel model)
 * making one abstract for MicroTAS based on numerical results of 20 um channel model.
 * designing herringbone groove for better trapping <br)
 * investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
 * IRB still in revision.
 * Cathie in charge of PCR1 paper right now.
 * Qingqing has written the introduction
 * tested the PCR efficiency for different designs of channel.
 * new design 1 has much better performance than the original one, while new design 2 has worse performance
 * evaluation of PCR efficiency from fluorescent signal and molarity is different.
 * will make figures for PCR1 paper.
 * developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)
 * making one abstract for MicroTAS (similar to the abstract of AACC)
 * Cathie submitted One pager Whitepaper. Waiting for response.
 * convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?
 * Preliminary no-valve design for 25 microliters.
 * 20 more test chips to Sonali (AACC poster).
 * Sonali try to do 10 microliters in the tube
 * Megan
 * Test on Thu, Fri worked but failure after some cycles.
 * Need to enhance bonding (e.g. Ar or O2 ashing)
 * STILL NEED TO SCHEDULE MEETING

† Silica Optimization (Lambda):
 * The following will be submitted monday
 * total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
 * Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
 * Emission spectrum for the positive samples split into 2 groups, one with pico green and the other without at 488nm.
 * Chip ran with total of 5ng of NA, pico green using the low range standard curve


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