Registry of Standard Biological Models/Basic Component Models/Repressed Promoter RBS Coupled

CellML structure (CellML 1.1 spec)

 * Component: RepressedPromoterRBSCoupled
 * Units:
 * Imported from Environment component
 * Variables:
 * nb-gene-copies (public interface = in / init value = 1.0)
 * maxSynthesisRate (public interface = none / init value = XXX)
 * Kd (dissociation constant) (public interface = none / init value = XXX)
 * n (Hill Coefficient) (public interface = none / init value = XXX)
 * synthesisRate (public interface = out / init value = XXX)
 * repressor (public interface = in )
 * MathML
 * synthesisRate = \frac{nbGeneCopies*maxSynthesisRate}{K_d^{n}+[repressor]^{n}}

CellML File








     



 

Comments

 * This component is simply a container providing the synthesis rate specific to a constitutive promoter coupled to a RBS and a given level of the repressor
 * To characterize this part you need to get {maxSynthesisRate} and {sensitivity}. It can be achieved experimentally using a quantitative GFP assay. Measurement of GFP levels should be done at steady state with different levels of the repressor.