User:Karmella Haynes/Notebook/BioBrick cloning/2009/11/24

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11/24/09

 * &#x2713; Minipreps: KAH011(2), 012(2), 93(2), 94(2), 95(2)
 * &#x2713; Sequencing order (same day): KAH011, 012
 * &#x2713; Assemblies: KAH96, 97, 98, 99, 100, 101
 * &#x2713; Cultures: Re-pick KAH94(4), 95(4)

Minipreps > Check with E/P digests

Assemblies
 * 1) KAH96: 5xGal4/(S/P)/~3300 &#x2713; + (1) HSVtkTATA-K-2xYFP-NLSstop2-polyA-2xHS4(KAH86)/(X/P/ScaI)/2726
 * 2) KAH97: (2) 5xGal4-Intron(KAH42)/(S/P)/~4875 + "
 * 3) KAH98: (3) 5xGal4-2xIntron(KAH47)/(S/P)/~6457 + "
 * 4) KAH99: (4) Ubc-5xGal4 (KAH83)/(E/S)/443 + (7) K-PEST-2xAmCyan-NLS-stop-polyA(KAH82)/(E/X)/~5664
 * 5) KAH100: (5) HPK-5xGal4 (KAH84)/(E/S)/615 + "
 * 6) KAH101: (6) CMV-5xGal4 (KAH85)/(E/S)/687 + "

> Digests (Fermentas FD) --> Use triple digest for KAH86

> Measure conc.'s


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