User:Karmella Haynes/Notebook/Polycomb project/2010/01/11

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01/11/10

 * &#x2713; Polycomb-ATF lines: split and test w/ doxycycline

Polycomb-ATF lines > Split each clone into two wells in 12-well dishes; Use zeo/blast/puro maintenance medium (1 mL/well total) > Use 1μg/mL Dox in second well > 1 12-well dish per transfection construct KAH126-133 (8 total) > Check for dox-inducible RFP expression after 24 hours


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