Klapperich Lab:Notebook/Lab Meeting Notes/2009/09/08

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8 September 2009 Lab Meeting
‡ Announcements
 * Attending:
 * Missing:
 * Presentation: Cathie: Fill in the Blanks Document on FLU. Delegation of Tasks. Definition of "Tiger Teams."

‡ Flu R01:Integration '''* Need to update IBC to include rDNA work. ''' † Sample Concentration (Lead: Jane, Team: Jaephil) † SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) † PCR - CMI (Lead: Qingqing) † HDA (Lead: Jaephil, Team:Sonali) '''‡C. diff Project''' (Cathie, Sonali, Satish Singh, His post doc )
 * Volume collection issues at this time. Where is the loss?
 * Alexa Fluo-488 succinimidyl ester (Molecular Probes) stains the flu virus fluorescent.
 * The current problem is not how to visualize virus right now, because the design is clearly not optimized for collecting all the samples at the outlet. The major loss is from sample handling instead of from non specific binding to the material.
 * RNA extraction troubleshooting. Do deinhibition of monolith components. Test those with the Ambion RNAse kit.
 * QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR.
 * CMK: PCR 2 paper draft.
 * CMK: PCR 1 draft. Analytical Chem.
 * on-chip PCR on a series of diluted lambda phage DNA. LOD for ABI is 10-9g/ul
 * on-chip PCR have the same sensitivity.
 * With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good.
 * New primers?
 * Double PCR on chip.
 * Start planning R01 for Submission on 2/5/10. MM, JD, CMK.
 * JD working on paper. Will deliver to MM this week.
 * Figures discussion.
 * Meeting 12:30-1:30pm every other Wed. in my office.

‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing) ‡ Agilent Automated Sample Preparation (Lead: Alex) ‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)  ‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks) * set up 2 week check in meeting with team. ‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)
 * Meeting 9am Monday 9/14 (every other week)
 * With Straws in Parallel at this point. Speeding up the assay.
 * Waiting for the machine.
 * Update on concentration of live bugs? MSSA?
 * Virus concentration (dialyzed against PBS, no SERS signal, dialyze against water now).
 * paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli.
 * New fabrcation in process.
 * took SEM images of parallelized designs, will show them if possible in the meeting.
 * Through SEM images, we found the smallest feature size is 1.2 um.
 * IRB approved.
 * Cathie submitted the companion BU IRB form for exemption.

‡ PATH Grant (Lead:Cathie, Team:Frank, TBA)
 * 12 mos, 24 mos. working prototypes of SNAP.


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