IGEM:MIT/2006/Notebook/2007-8-25

=Things to Do Today=

1. Add PCNB to GC samples- DONE

2. Run ALL 18 GC samples

3. Dilute J45995, J45996, B0015, and I7100 cultures 1:250 and grow them up as 25-mL cultures in 250-mL baffled flasks- DONE

4. Grow up cultures for 4 hours and 20 mins at 220 RPM at 37C- DONE

5. Dilute cultures into plate reader wells

Culture-OD600--Dilution

J459951-1.50---8.11 uL per 191.89 media

J459952-1.50---8.11 uL per 191.89 media

J459953-1.52---8 uL per 192 uL media

J459961-1.46---8.33 uL per 191.67 media

J459962-1.52---8 uL per 192 uL media

J459963-1.46---8.33 uL per 191.67 media

I71001--0.54---22.52 uL per 177.48 media

I71002--0.64---19 uL per 181 media

I71003--0.68---17.88 uL per 182.12 media

B00151--1.08---11.26 uL per 188.74 media

B00152--1.12---10.86 uL per 189.14 media

B00153--1.06---11.47 uL per 188.53 media