User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/07

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October 7th, 2010
1. Extract plasmid from the following strains:
 * pSB1A2 + J23101 + ΔRBS + GFP E004
 * pSB3K3 + J23101
 * pSB4A5 + MinBP
 * pSB1C3

2. Make the following restrictions:
 * pSB1C3 EcoRI-PstI restriction


 * EcoRI-PstI double restriction methods:
 * DNA -> 5 ul
 * Buffer 3 -> 2 ul (10% of total volume)
 * BSA -> 1 ul
 * EcoRI -> 1 ul
 * PstI -> 1 ul
 * HPLC -> 10 ul (to complete total volume of 20ul)
 * Incubate at 37º C for 4 hrs.


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