BM20

BM20 media


 * 10% low endotoxin FCS
 * 20% L-cell supernatant
 * 5% horse serum
 * 1% glutamine
 * 1% sodium pyruvate
 * no Abx
 * to final volume with low endo DMEM

1L
 * 630ml low endotoxin DMEM
 * 100ml low endotoxin FCS
 * 200ml L-cell supernatant
 * 50ml horse serum
 * 10ml glutamine
 * 10ml sodium pyruvate
 * No Abx