User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/02

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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HCO/LCO Adenita Culture Re-Amp PCR Purification with QIAquick
For failed sequencing samples 8, 12, G

http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2


 * see page 19.
 * 16ul PCR product left for cleaning. Resuspended in 50ul EB buffer.

Use 4.0ul = 15-20ng for Cycle Sequencing

HCO/LCO Adenita Culture Cycle Sequencing
For failed sequencing samples 8, 12, G