User:Mar/Notebook/2007-8-1

= Hot SHOT testing = rl119: a-e reelers; f het rl120: a,e,f reelers; b,g het, c,d inconsistent with ProtK method
 * material: 13 p00 mouse limbs of RL119 and RL120
 * incubation: 75µL/limb, 30 min
 * concentrations: range from 0.4 to 4.24 µg/mL (avg = 1.67; median = 1.75)
 * PCR: Green GoTaq (regular AWS program)
 * bands showed up pretty well, although weaker then with protK, and required photoshopping before reading

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