Shreffler:Notebook/Staff Meetings/2010/06/04

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] ShreffLab Staff Meetings
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Agenda Items
Alex's work
 * JAX
 * scanner works
 * need to make a plan to work with Kunal
 * gating strategy for template does not exclude DR currently
 * Alex will review protocol to come up with total number of basophil samples that require staining and request a quote from BD for cocktail (minus the CRTH2).
 * look into buying lot of CRTH2 from Miltenyi


 * PM
 * Alex getting data organized/ analyzed


 * R03
 * Reviewed data from 5 experiments (3/5 look like measurable DHR response)
 * tentatively looks as though DHR is suppressed by EDTA
 * Alex to export data from FJ to tables to complete analysis


 * Carbo
 * made a new template using CRTH2 -- suggests that there may be substantial DR expression!

Action Items

 * Wayne will talk to Ben about using qPCR more regularly (done)
 * Bert will compare LPS response
 * run fraction screening experiment on Ben's machine
 * repeat fraction screening experiment with pooled fractions
 * get larger column for bigger run
 * contact Steve about using HPLC (not done)