Fong:TFU

Overview
This liquid medium is for general culture of the actinomycete Thermobifida fusca. In this recipe, cellobiose is the sole carbon source at 0.5%. Both the amount and type of carbon source may be varied; cellulose, CMC, glucose, and xylan are all acceptable sources.

Materials
For one liter liquid medium


 * Macro Salts
 * 1.5g NaCl
 * 3.1g (NH)2SO4
 * 9.1g Na2HPO4
 * 0.9g KH2HPO4
 * 0.2g Yeast Extract (optional)
 * 5.0g Cellobiose
 * 20ml [Microsalts]
 * 10ml Biotin and Thiamine

Procedure

 * 1) Add macrosalts to 750ml diH2O
 * 2) *Heat as necessary.
 * 3) Adjust to 7.4 ± 0.2 pH
 * 4) Add additional ingredients
 * 5) Raise volume to 1L and cover
 * 6) Autoclave on liquid cycle for at least 30min.

Contact

 * Deng

or instead, discuss this protocol.