JCA062306-Construction of pJ23004

 PCR ca1002F/R on pSB1A3-I13521 (987 bp, EcoRI/PstI, Neb2) Sub into pSB1A3-I13521         (EcoRI/PstI, Neb2) Product is pJ23004

ca1002F	Forward EcoRI oligo to make XbaI blunter cassette cttctggaattcgcggccgcaactagtgtccctatcag ca1002R	Reverse PstI oligo to make SpeI blunter cassette gaagcctgcagcggccgctTctagAatataaacgcag 