Silver: Bacterial Transformation


 * 1) Thaw competent cells (TOP10 from Invitrogen) on ice.
 * 2) Place 10-15 µL cells in pre-chilled Eppendorf tubes.
 * 3) Add 2 µL BioBrick vector (either native or ligated; so that vector is ~10-20% of the final volume), and chill on ice for 30 min.
 * 4) *Do not pipet or vortex.
 * 5) Heat shock at 42 °C for 30 s.
 * 6) Incubate on ice for 2 min.
 * 7) Add 170 µL SOC medium (~10 x volume; Invitrogen), and shake at 37 °C for 15 min for Amp selection, or 30 min for Kan selection.
 * 8) Add all media to a selectable marker LB plate.  Use glass beads to streak the plate.
 * 9) Incubate at 37 °C.  Transformants should appear within 12 hrs.