Template:SBB10 ConstructionFiles JoseG sbb37

Biobricking of Pcon PCR JG005-F and JG006-R on pBca1100-Bca1152   (66 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144                     (EcoRI/BamHI, 910+2170, L) Product is Pcon-pBjk2741    {Pcon-J23100} JG005-F  Cloning of papC   ccataGAATTCgtccAGATCTttgacggctagctcagtcct JG006-R  Cloning of papC   ctgatGGATCgctagcactgtacctaggac



JCA Notes
Eco/Bam transfer pBca1100-Bca1152 Sub into pBjk2741-Bca1144                     (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-Bca1152   {Pcon-J23100}
 * This just needs to be eco/bam transferred, not pcr'd
 * Nomenclature of plasmid changed
 * Modified entry on clotho