IGEM:IMPERIAL/2007/Calendar/2007-9-3

We are in Week 9  of the iGEM project

Project Calendar
name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7

Testing of DNA constructs

 * Raw results up on the wiki
 * Refinfed data and graphs currently being put up
 * Data to be given to modelling team for analysis
 * Problems with staggering of experiments - results not very useful
 * Talk to supervisors
 * Try to get longer access to equipment (fluorometer, incubator etc)
 * Find out methods to reduce error and variability in results

Cloning

 * pTet and pLux constructs have been cloned
 * > 300&micro;g of each DNA made - enough for all experiments
 * LuxR arrived - might be transformed by Wed
 * Protocol team to talk to clonning team about details
 * pT7-GFP construct being cloned in BL21

Vesicles

 * New procedure being adapted which works well- not leaving overnight and using 10ml suspension
 * Saves ingredients
 * No need to leave overnight
 * Cell extract will be tested in vesicles tomorrow
 * FACS machine for counting vesicles - not sure if can get access
 * No concrete decision yet made on where vesicles fit into the project
 * Meeting between Dirk (captain vesicles), Ben (captain ID) and Alex (captain CBD) to discuss feasibility of vesicles

Wiki

 * In the process of being redesigned - Alex and Anthony
 * Make wiki more organise and facilitate navigation for visitors
 * Talked to Vincent
 * Talking to individual groups about sections relevant to them and proposed layout

AOB

 * Characterisation of chassis - need to focus on it a bit more
 * Modelling and testing within in-vitro and vesicles
 * Use literature rather than experiments to characterise chassis being used
 * Use the information in in-veso teams(old oraganisation) page