User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/02/03

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Ran gel of yesterday's PCR
 * 1hr 20 min @ 120V
 * Somewhat inconclusive results, but 5.1 is definitely positive. Will most likely confirm via cross to Flp-out.
 * Observed tdt-Myb, gfp-PCNA
 * Too much background in tdt-Myb channel
 * Dissected tdt-Myb, gfp-PCNA
 * Orange and Peach/Yellow eyes
 * Fix with 4% Formaldehyde PBS Solution
 * 20 minutes
 * Wash 3 x 3 minutes in 1x PBS-T
 * Incubate 25 minutes with PBS 0.6% Triton.
 * Wash once with 1x PBS-T
 * Block O/N 1:1000 in 2% NGS


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