User:Sarah Signor/Notebook/Genetics of Pigmentation/2011/01/12

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Jan 12
I cleaned up the digested RAD library after P1 adapter ligation. I included my four D. pseudoananassae samples. Concentrations are all fine:
 * Library Testing

RAD: 306 ng/ul in 80 ul Diluted Dpp: 31.74 in 80 ul Diluted Dpn: 44 in 80 ul Not diluted Dpp: 55.79 ul in 80 ul Not diluted Dpn 45.88 with the best 260/280

Suprisingly little difference between the diluted and undiluted samples. Appointment at the Bioruptor tomorrow.


 * Malpal

I created a list of the genes surrounding marker 4_7096_611_12485. As the markers are not in the same order as ananassae it is difficult to tell exactly what genes are there, I extended my list to around where the next marker would be in ananassae. There are a number of candidates, mostly not available from Japan or Bloomington. File is entitled Ana_genes_by_top_marker1_12.xls


 * }