User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/04/26

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April 26th, 2010
I made a restriction with EcoRI and XbaI to the last restriction of plasmid with the biobrick BBa_B0015. The restriction was made with 40μL, which were composed of:

20μL DNA

4μL Buffer 2

1μL each enzyme

1μL BSA

14μL H2O

To observe if the restriction and the plasmid were OK I made an agarose Gel to .08.

Ladder | DNA Restricted 4μL | Plasmid 5μL | Plasmid Paz(control) 5μL | Ladder

Gel 90V 40min + 80V 10min

The plasmid we were looking for is pSB1AK3 with 100-300 copy number per cell. 3189bp

We got as a result two fragments of 1kb(???) and 3kb instead the 3kb and 100bp fragments that we expected.


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