User:Karmella Haynes/Notebook/BioBrick cloning/2011/01/17

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01/17/11

 * &#x2713; Minipreps: KAH173(2), 174(2)
 * &#x2713; Assemblies: KAH175-178

Minipreps > Check with E/P digests

Assemblies
 * 1) KAH175: (1) KAH174/(S/P)/~5190 + polyA2/(X/P)/225 &#x2713;
 * 2) KAH176: (2) KAH173/(S/P)/~6160 + polyA2/(X/P)/225 &#x2713;
 * 3) KAH177: " + (3) KAH171/(X/P)/418
 * 4) KAH178: " + (4) KAH172/(X/P)/429

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

--> ~10 min./ rt; add to 30 μL DH5α turbo


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