IGEM:Cambridge/2008/Notebook/Voltage/2008/08/01

{| width="800"
 * style="background-color: #EEE"|[[Image:Untitled-2.jpg‎ |390px]] ...........   Electric Output
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Mutants

 * The OD600 of the mutants grown in different K+ concentrations was taken again.

osmY

 * Plasmid mini prep was done.
 * Gel was run, showing clear band at around 450bp, indicating osmY with BioBrick primer prefix and suffix.
 * Glycerol stocks were made of the TOP10 cultures with the osmY-containing plasmid.
 * More LB was added to the TOP10 cultures and they were put in the incubator.

Plasmid

 * PSB4C5 was extracted from DB31, into 100μl SDW - final conc. 106.5ng/μl.


 * }