User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/List of Supplies

List of needed supplies to carry out the listed protocols:

Clean RNA technique

 * DEPC or DMPC (DMPC is less carcinogenic) for making RNAse free solutions/containers. --> Bought from Sigma 6.23
 * Peroxide --> bottles of unknown age, but chemical is still reactive. might as well use, since it's only used as a cleaning agent.
 * Deionized glyoxal --> Bought from Sigma 6.23

Sean Moore's RNA Extraction Protocol

 * rna- strain of Top10 --> If we can get this great, if not, no worries.
 * BPER lysis soln --> Sean says this is optional but still uses is as his solvent.  We have this.
 * MgOAc --> on RTS shelf
 * Lysozyme --> can't find, let's buy more from Pierce I believe (BC)
 * DNAaseI --> NEB stuff in shared enzymes box. Buffer conditions differ from Sean's lysis soln, but should be OK.
 * Linear polyacrylamide (can't contain bis) --> bought from Sigma 6.23
 * Ambion's phenol/choloroform mix (pH 4.5) --> bought from Ambio 6.23

Northern blotting (Endy) Protocol

 * SybrGold --> have some aliquots in Barry/Jason's -20
 * BPTE buffer:
 * PIPES (RTS shelf)
 * Bis-Tris (RTS shelf)
 * EDTA (DE shelf)
 * RNA loading buffer:
 * 95% deionized formamide
 * 0.025% Bromophenol blue (solid on RTS shelf)
 * 0.025% xylene cyanol FF (solid on RTS shelf)
 * TurboBlotter apparatus --> asked Heather where this is.... waiting for reply
 * Nytran SuPerCHarge or Immobilon NY+ nylon membranes? --> might ask Sean what works better, or if it even matters...