IGEM:IMPERIAL/2006/LabCalendar/2006-8-16

12D->2H

 * The gel that was run yesterday was inconclusive as to whether the DNA has the problem or the enzyme.
 * We will run 7A using the same procedure to see if it is cutting the plamid at all. We are again using the same enzymes with yellow buffer
 * SpeI & PstI were able to successfully cut 7A, so it is not a problem with the enzyme!
 * We will try re-electroporating part 2H again using the DNA from the parts registry to see if we can get it to work tomorrow





Reculture J37016

 * Plates were quite good and we managed to isolate single colonies
 * We took a total of 4 cultures to miniprep tomorrow or later this afternoon to see if we get what we expect.

Ligation of 6B+RS+12D+24A

 * The insert and vector were removed from the overnight gel
 * 20 uL ligation performed

Electroporations

 * J37015RS (6B->RS->12D->24A)
 * 1M B0021 removed from iGEM plate
 * 4G S01656 removed from iGEM plate 2, grown on kanamycin plate
 * 2H I13033 re-electroporated to see if the SpeI site has mutated on the gene
 * Didn't bother with adding LB, since we only have LB Amp and no Kanamycin. So just plated the electrocompetent cells straight onto plate.  Hopefully this doesn't affect growth.

Set up Maxipreps

 * 6B F2620 (ran out of maxiprep)
 * 1I B0015 (remaxiprep, since whereabouts of previous maxiprep unknown)

To do tomorrow

 * Maxiprep parts above
 * Reculture the electoporations into liquid media
 * Ligate 1I and 9G to make part 2H, in case 2H fails yet again
 * Miniprep the parts from electroporation, if possible
 * Miniprep J37016 which is currently in shaker since 11 am Wednesday