User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/26

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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HCO LCO Screening
Test Ramp PCR on Cultured samples 1-7

Load 1.0ul sample

Alien Genes New Primer Screen
Loaded 2.0ul of each sample



Testing new primers on ricciae and vaga
These primers were designed to amplify all four copies of the gene, using data from A. vaga and P. roseola.


 * PCR conditions 95C 3m (93C 5s;60C 1m)x35

Gel

 * Unless specified, the order of the sapmles is ricciae then vaga
 * all of the primers worked, except sacairs and a15, which did not cross-amplify
 * maybe should also try them on Philodena?