IGEM:IMPERIAL/2007/Notebook/2007-8-10

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/15 view=threemonths format=%name/%year-%month-%day weekstart=7

Preparation of Stock Solutions

 * 1x 500 ml of 2M Tris-acetate pH 8.2
 * 1x 500 ml of Buffer A for preparation of S30/S12 cell extract
 * 1x 500 ml of Buffer B for preparation of S30/S12 cell extract
 * 1x 50 ml of 2mM amino acids

Miniprep of Biobricks

 * 1) Minipreped 2x 14 Biobricks

Protocols can be found at Miniprep in the general protocols page

Agarose Gel Electrophoresis of Biobricks

 * 1) Checked 2x 14 Biobricks on 1% agarose gel

Protocols can be found at Electrophoresis in the general protocols page