User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/19

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Counting and splitting cells
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary
A repeat of the previous experiment.

Materials

 * petridishes (10 mL)
 * 6-wells plates (2 mL)
 * 24-wells plates (0,5 mL)
 * DMEM S+

Method

 * Cells are collected in DMEM S+ as described in Splitting cells and counted as described in  Counting cells
 * Cells are split over several plates
 * 4 petridishes (10 mL) receive 500.000 cells
 * 1 6-wells plate (2 mL) receives 100.000 cells per well
 * 2 24-wells plates (0.5 mL) receive 20.000 cells per mL

Results
Cultures contained 57.25×104 cells per mL (17175×103 total)
 * 6-well (2 mL per well)
 * 12.78 mL DMEM S+
 * 1.22 mL cell suspension
 * Petridish (10 mL per plate)
 * 45.64 mL DMEM S+
 * 4.36 mL cell suspension
 * 24-well (0.5 mL per well)
 * 24,3 mL DMEM S+
 * 1.7 mL cell suspension

Related

 * Splitting cells
 * Counting cells


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