IGEM:IMPERIAL/2006/Protocols/western blot

Western Blot Protocol

 * Grow overnight in a 5 ml culture
 * Make a fresh day culture 400 of overnight culture + 4.6 ml of fresh medium (LB)
 * Grow fresh culture for 1.5hrs
 * Remove 1ml (This will be your control)
 * Add IPTG to the remaining 4ml - Final conc should be 0.5-1 milimolar (So add 4 of 1 molar IPTG)
 * Grow for 2-3 hrs
 * Centrifuge down
 * Add Lysis Buffers
 * Run on gel

You should get this



Source http://www.cdc.gov/ncidod/eid/vol7no1/hussainG1.htm