User:Anthony Salvagno/Notebook/Research/2009/12/21/Tethering Marathon

Intro
Koch says I need to perfect the technique and so I will do just that. Now I already am pretty good at the general technique, meaning give me things to flow and I will flow like nobody's business. I just need to consistently get tethered beads in almost every field of view in a slide. I'm pretty sure the DNA is good, so my problem could lie in the beads or the anti-dig.

To make sure I will order some new anti-dig today and work off different preparations of beads. First I will make 2 new samples of beads. One sample I will try and sonicate the beads really well. In another sample I will try to vortex the beads really well. I will then tether several times with each bead prep sample and check my results taking images from a few FOV's.

Bead Prep
I will alter the protocol as documented in the [[Media:Washingbeads.pdf|Bead Washing PDF]] I found from Bangs Labs on Fri. The normal protocol is 3 washes with spin-downs at 6600g for 5 min, but according to Bangs's Technote it should be 2200g for 15 min so I will try that. See here.