IGEM:MIT/2006/Notebook/2006-8-19

SAGD stuff

 * 1) 16 minipreps of pchBA-B0015 muts
 * 2) digestions:
 * 3) *pchBA-B0015 muts with: XP (RUN A GEL!! b/c pchA has a PstI site)
 * 4) ligations: all good
 * 5) transformations: on A/C plates

IAGD stuff

 * 1) digestions:
 * 2) *good 30-Bat2-30 with: ES- i'd still do this and ligate it to thi3
 * 3) *THI3 with: XP

shifts

 * 1) Kate: minipreps of pchBA and LC/glycerol of Q04400 and pUCP22 -done
 * 2) *miniprepped 15 pchBA-B0015 muts plus Q04400 -- all good concentrations. i re-labeled the muts 1-15. do not bleach the LC tubes so that we can keep track of the ABC system if needed.
 * 3) *Veena: did not make any glycerols b/c it makes sense to wait and glycerol good ones after reading gel in pm
 * 4) *Veena/Stephen: did not select colonies for LCs b/c it is too early (risk overgrowing Kate/Bo think) so have labeled and poured 21 LC tubes with proper LB -- if someone in pm could put in colonies would be good
 * 5) *Call me with any questions
 * 6) Stephen: plate reader and osmY analysis (+ sunday)
 * 7) Andre: LCs of IK plates and Glycerols of IK cells without substrate, smelling of IK cells with substrate, set-up new experiments (??)
 * Bo: 18 digests, pour and load 2 gels
 * 1) Veena: read gel, design good ligations (remember to connect both RBS), ligate, transformation