User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/24

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=WetLab: LovTap + promoters=

Transformation
The ligations from 230810 were transformed into DH5α competent cells following the next protocol.
 * 20' on ice
 * 1' @ 42ºC
 * 5' on ice


 * 1h 1mL of LB
 * 1' @ 3000rmp

As a positive control was used the plasmid N.19[Am]

Ligation
A new set of ligations were done, this time with the colony_9 of LovTap. The same protocol was followed.

Ligation Protocol

 * DNA(LovTap) ..............6μL
 * Plasmid (promoter) .....2μL
 * Buffer 10X T4 Ligase ...2μL
 * Enzyme T4 Ligase .....1μL
 * HPLC ........................9μL

TOTAL 20μL

The mixture were left ON @ 16ºC


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