IGEM:MIT/2006/Notebook/2007-7-19

=To Do Today=

1. Add PCNB to all GC samples- DONE

2. Run all EIGHTEEN GC samples- DONE

3. Take out plate of IlvE- strain- DONE

4. Take out new LCs of J45120 and J45181- DONE

5. Adjust the pH of Tom's media as Tom suggested- DONE (looks to be ~7.7)

6. Grow up the same cultures on Tom's pH-adjusted media- DONE

7. Add 50 uL water to tube containing Japanese plasmid- DONE

8. Nanodrop the contents of the tube containing Japanese plasmid- DONE (21.6 ng/uL DNA)

=Results=

J45120

Hour---OD600Methyl Salicylate (ppm)---Methyl Salicylate (ppm)/OD600

5--0.14-0.0---0.00

6--0.42-0.0---0.00

7--0.84-0.0---0.00

8--1.38-1.0---0.72

9--1.84-23.1--12.6

102.20--28.7--13.1

112.26--26.1--11.6

122.40--27.5--11.5

132.48--18.8--7.58

J45181

Hour--OD600-Methyl Salicylate (ppm)---Methyl Salicylate (ppm)/OD600

5--0.24-0.0---0.00

6--0.50-1.1---2.20

7--0.92-8.7---9.46

8--1.46-18.5--12.7

9--1.82-22.3--12.3

102.30--25.9--11.3

112.20--24.8--11.3

122.38--24.8--10.4

132.52--18.3--7.26