SIL071906-Construction of pSB1A2-J23014

[trbCf] PCR JL14/JL15 on Olambda  (679bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521            (EcoRI/SpeI, Neb2, Large 2062) Product is pSB1A2-J23014 - JL14 Forward EcoRI oligo to bioBrick trbC: CCTAGGAATTCGCGGCCGCTTCTAGatgaagctgagtatgaaatc JL15 Reverse SpeI oligo to bioBrick trbC: gacttACTAGTcgtttcatttcccggaatctc

JL16 Forward trbCf 20mer gctggcgttaccagatggtg JL17 Reverse trbCf 20mer gccctgaatactgctgcttcc