User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/05/06

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May 06th, 2010
I am going to make a double restriction with EcoRI and XbaI in order to open the plasmid pSB1AK3 (containing a doble terminator). We need the DNA more contcentrated.

20μL DNA

4μL Buffer

1μL BSA

2μL each enzyme

H2O

Total : 40μL

And I let the restriction sample incubating from 6h44pm and 37°C.


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