Registry/Measurement kit/Notebook/2007-8-3

E0240

 * Trying mutagenic PCR of E0240.
 * Used 17 cycles. Don't know what # is optimal. Stratagene recommends 12, but people seem to use ~18.
 * Too many cycles -> more junk DNA.
 * Digesting with DpnI. Felix suggested digesting for >2h.

Transformations

 * Later will try re-transforming into another set of competent Top10 cells.
 * Should make more MG1655.
 * Re-tried yesterday's transformations into Top10s.
 * O/n of MG1655 is growing.