IGEM:Harvard/2006/Stock solutions

Calcium chloride
To make 100 mL of 0.10 M:
 * 1.47 g CaCl2 &middot; 2H2O
 * add dH2O to 100 mL

Magnesium chloride
To make 100 mL of 0.10 M:
 * 2.03 g MgCl2 &middot; 6H2O
 * add dH2O to 100 mL

Potassium chloride
To make 100 mL of 1.0 M:
 * 7.46 g KCl
 * add dH2O to 100 mL

Sodium chloride
To make 100 mL of 1.0 M:
 * 5.84 g NaCl
 * add dH2O to 100 mL

Tris-acetate
To make 100 mL of 2.0 M:
 * 24.2 g Tris
 * add dH2O to 90 mL
 * add glacial acetic acid until desired pH (pH 7.4 is about 7 mL of acid)
 * add dH2O to 100 mL

Bock's selection buffer
To make 40 mL of 5x:


 * 2 mL of 2 M Tris-acetate pH 7.4 (final concentration: 100 mM)
 * 28 mL of 1 M NaCl (final concentration: 0.7 M)
 * 1 mL of 1 M KCl (final concentration: 25 mM)
 * 2 mL of 0.1 M CaCl2 (final concentration: 5 mM)
 * 2 mL of 0.1 M MgCl2 (final concentration: 5 mM)
 * 5 mL dH2O

Reference: # bock pmid=1741036

Bittker binding buffer
(buffer used by Bittker et al. in selecting for aptamer that binds streptavidin)


 * 150 mM NaCl
 * 50 mM Tris HCl pH 8.0
 * 10 mM MgCl2