Template:SBB10 ConstructionFiles JoseG sbb30

Biobricking of O99 rep Gene PCR Pcon-F and Pcom-R on pEC52. (1236 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144                     (EcoRI/BamHI, 910+2170, L) Product is Pcon-pBjk2741    {5'UTR_repO99!} JG001-F  Cloning of O99 rep Gene  ccataGAATTCgtccAGATCTaaaaacgattctgacgcatttt JG002-R  Cloning of  O99 rep Gene   ctgatGGATCCctactctacaagacctcgtttt



JCA Notes
Biobricking of O99 rep Gene PCR JG001-F and JG002-R on pEC52     (1235 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144            (EcoRI/BamHI, 910+2472, L) Product is pBca9523-sbb30    {5'UTR_repO99!}
 * Needs to be with vector pBca9523
 * Oligo names are incorrect in description
 * Extended oligos to get to a g/c
 * Name of plasmid should be vectorName-partName (pBca9523-sbb30)
 * Corrected Clotho entry
 * Otherwise correct

JG001-F  Cloning of O99 rep Gene ccataGAATTCatgAGATCTaaaaacgattctgacgcattttttatg JG002-R  Cloning of  O99 rep Gene CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc