User:Robwarden/Notes from 20.109

=20.109 Notes= These are my notes from 20.109, taken Spring 2007

Last Name
Warden

First Name
Robert

Preferred name
Rob

Course/Minor
20/5

Year of Graduation
2009

Telephone #
(248) 802-7023

Email
rw27 AT mit DOT edu

I have taken
5.07 (Biochemistry I) 7.03 (Genetics)

I am taking
20.109 (Lab Fundamentals of Biological Engineering) 5.03  (Inorganic Chemistry) 5.08  (Biochemistry II) 6.00  (Intro to Computer Science) 14.01 (Microeconomics)

Do you have any experience culturing cells (mammalian, yeast or microbial)? Yes, LNCaP and HMEC human cancer cells

Do you have any experience in molecular biology (electrophoresis, PCR, etc)? Yes, electrophoresis, westerns, PCR, restriction digests, ligations, transformations, transfections

Please briefly describe any previous laboratory experience
Summer UROP creating a vector with a tagged version of the growth receptor Her2, to study crosstalk in prostate cancer.

"Spit on a bat before using it for the 1st time to make it lucky."
I would give a player 5 bats that have been spit on and 5 that have not. He would then rotate through the bats, not knowing which were lucky. I would then count the number of home runs hit with each bat, and see if the spit on bats had more home runs attributed to them.

Wiki Notebook

 * Expression Engineering
 * Day 1
 * Choosing a Saga Gene
 * Primer Design
 * PCR
 * Day 2
 * Counting Yeast
 * Plating Yeast
 * Competent Cells
 * Transformation
 * Day 3
 * Day 4
 * Day 5
 * Day 6
 * Biomaterials Engineering
 * Day 1
 * Day 2
 * Day 3
 * Day 4

For Next Time

 * Genome Engineering
 * M13 Redo
 * Day 1
 * Day 2
 * Day 3
 * Day 4
 * Biophysical Signal Measurement
 * Day 1
 * Day 2
 * Expression Engineering
 * Writing Assignment
 * Day 1
 * Biomaterials Engineering
 * Research Proposal
 * Day 1
 * Day 2