User:Karmella Haynes/Notebook/BioBrick cloning

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Project Description/Abstract
Creation of parts & assembly of constructs for all projects
 * Polycomb Project
 * Test Gal4-ATF's: built &#x2713;, expression test &#x2713; , functional test &#x2713;
 * Polycomb-ATF's (with myc tag): built &#x2713;, stable lines &#x2713;
 * Histone methylation reporters: built &#x2713;
 * Neo translation induction test constructs: built &#x2713;, functional test &#x2713; (done, but uncertain outcome)
 * Gal4-PEST-EED neo-inducible expression constructs: built &#x2713;, functional test (failed)
 * Full-length histone methylation sensors (+/- DPRE, constitutive Gal4-EED): built &#x2713;, expression test (in progress)
 * Polycomb-ATF no-VP64 controls: built &#x2713;, stable lines &#x2713;
 * ES cell expression constructs (10 total): building (in progress)


 * miR Sensor Project
 * RFP reporter/ repressors: built &#x2713;, expression test &#x2713;
 * CFP reporters: built &#x2713;, expression test &#x2713;
 * 9 pair-wise combos of RFP/CFP: built &#x2713;, expression test &#x2713;
 * 0, 5, 10x UAS site Target constructs: Francesca
 * miR-34 Repressor constructs: Francesca
 * New pcDNA3.1+/ΔCMV,hygro & pcDNA3.1+/ΔCMV,zeo vectors: Andrew
 * YFP-miR-luc/ CFP Target/ RFP-Repressor stable line: Andrew
 * U2OS plain/ miR-34 sensor line: Andrew


 * Fly miR Sensor Project
 * Basic parts: in progress
 * Transient transfections of BioBricked fluors: pending