User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/04/14

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April 14th, 2010
17: pSB1C3 18: pSB1T3 19: BBa_I51020 30: pSB4A5Strains were grown in LB medium with the correspondent antibiotic for each plasmid. Double restriction methods:
 * 1) Prepare and grow strains with the required plasmids:
 * 1) Extract plasmids with Roche kit.
 * 2) Double resrtiction to extracted plasmids with EcoRI and Pst1.


 * DNA -> 5ul
 * Buffer 2 -> 2ul (10% of total volume)
 * BSA (required by PstI) -> 1ul
 * EcoRI -> 1ul
 * PstI -> 1ul
 * HPLC -> 10ul (to complete total volume of 20ul)
 * *Incubate at 37ºC overnight


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