IGEM:Harvard/2006/Adaptamers/Oligos

 News and Updates Notebook Oligos Code Plans Overview (for site visitors) Literature  

v.1

 * Oligos (8 ssDNA sequences):

SN = streptavidin aptamer + N bp 3' tail

TN = thrombin aptamer + N bp 3' tail

S5

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAG 3'

S20:

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTC 3'

S35:

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTCACTTCTGCAGCAATA 3'

S50:

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTCACTTCTGCAGCAATAGAATTAAGCTTGTCC 3'

T5:

5' GGTTGGTGTGGTTGGACACT 3'

T20:

5' GGTTGGTGTGGTTGGACACTGAACCTCTAGATGCT 3'

T35:

5' GGTTGGTGTGGTTGGACACTTATTGCTGCAGAAGTGAACCTCTAGATGCT 3'

T50:

5' GGTTGGTGTGGTTGGACACTGGACAAGCTTAATTCTATTGCTGCAGAAGTGAACCTCTAGATGCT 3'


 * Alignment

+20: 5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTC                       3' 3'                                             TCGTAGATCTCCAAGTCACAGGTTGGTGTGGTTGG    5' +35: 5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTCACTTCTGCAGCAATA 				3' 3'                                             TCGTAGATCTCCAAGTGAAGACGTCGTTATTCACAGGTTGGTGTGGTTGG      5' +50: 5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGCATAGAGCATCTAGAGGTTCACTTCTGCAGCAATAGAATTAAGCTTGTCC			 3' 3'                                             TCGTAGATCTCCAAGTGAAGACGTCGTTATCTTAATTCGAACAGGTCACAGGTTGGTGTGGTTGG 5'

v.2
T50.2

5' GGTTGGTGTGGTTGGAAGTAGTATGGAAAGGATTAGCAATGATAGGCATATTTATACGGCTCGGA 3'

T35.2

5' GGTTGGTGTGGTTGGAAGTAGTATGGAAAGGATTAGCAATGATAGGCATA 3'

T20.2

5' GGTTGGTGTGGTTGGAAGTAGTATGGAAAGGATTA 3'

S50.2

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGTTCTTTCCGAGCCGTATAAATATGCCTATCATTGCTAATCCTTTCCATAC 3'

S35.2

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGTTAAATATGCCTATCATTGCTAATCCTTTCCATAC 3'

S20.2

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGTTTGCTAATCCTTTCCATAC 3'

A15

5' TCTGTGAGACGACGCACCGGTCGCAGGTTTTGTCTCACAGTTGCTTTCCATTCTACATATGAAGGTTGGTGTGGTTGGAAGTAATGTAGAATGGAAAG 3'


 * Alignment

A50.2

GGTTGGTGTGGTTGGAAGTAGTATGGAAAGGATTAGCAATGATAGGCATATTTATACGGCTCGGA CATACCTTTCCTAATCGTTACTATCCGTATAAATATGCCGAGCCTTTCTTGACACTCTGTTTTGGACGCTGGCCACGCAGCAGAGTGTCT

A35.2

GGTTGGTGTGGTTGGAAGTAGTATGGAAAGGATTAGCAATGATAGGCATA CATACCTTTCCTAATCGTTACTATCCGTATAAATTGACACTCTGTTTTGGACGCTGGCCACGCAGCAGAGTGTCT

A20.2 GGTTGGTGTGGTTGGAAGTAGTATGGAAAGGATTA CATACCTTTCCTAATCGTTTGACACTCTGTTTTGGACGCTGGCCACGCAGCAGAGTGTCT

Biotinylated oligos
Biotinylated oligos as positive control in viscosity experiment:

5' and 3' biotinylated:

B35.0

5' GAGTTATGATTAGGCGGACGGTCTGAACAAGGTTT 3'

5' biotinylated:

B35.1

5' TGAGTTATGATTAGGCGGACGGTCTGAACAAGGTTT 3'

B35.2

5' TAAACCTTGTTCAGACCGTCCGCCTAATCATAACTC 3'

Strand Displacement Oligos
R20.20

5' TAATCCTTTCCATACTACTT 3'

R20.35

5' TAATCCTTTCCATACTACTTCCAACCACACCAACC 3'

T20-R20.20

5' GGTTGGTGTGGTTGGAAGTAGTATGGAAAGGATTA 3' 3' 	         TTCATCATACCTTTCCTAAT 5'

T20-R20.35

5' GGTTGGTGTGGTTGGAAGTAGTATGGAAAGGATTA 3' 3' CCAACCACACCAACCTTCATCATACCTTTCCTAAT 5'