Cconboy:Promoter Characterization/Constructs

Constructs in this study were assembled from parts in the Regisry via standard assembly. All constructs were cloned onto pSB1A2, a high copy plasmid, in MC4100.



Fig.1: Schematic of Promoter Characterization Constructs

{| border = "1" cellpadding="5" cellspacing="0" align="left" ! Construct !! Promoter !! Description
 * + Table 1: Promoter Characterization Constructs
 * BBa_I6030 || R0010 || LacI regulated ||
 * BBa_I6031 || R0011 || Hybrid promoter based on lambda pL, repressed by LacI ||
 * BBa_I6032 || R0040 || Hybrid promoter based on cI, repressed by TetR ||
 * BBa_I6034 || R0050 || HK022 cI regulated ||
 * BBa_I6036 || R0051 || Lambda cI regulated ||
 * BBa_I6038 || R0052 || 434 cI regulated ||
 * BBa_I6040 || R0053 || p22 cII regulated ||
 * BBa_I6042 || R0062 || lux pR from V.fischeri, activated by HSL-bound LuxR ||
 * BBa_I6044 || R0063 || lux pL from V.fischeri, repressed by HSL-bound LuxR ||
 * BBa_I6046 || R0065 || Hybrid promoter, repressed by lambda cI, activated by HSL-bound LuxR ||
 * BBa_I6048 || R1050 || Standard promoter, repressed by HK022 cI ||
 * BBa_I6050 || R1051 || Standard promoter, repressed by lambda cI ||
 * BBa_I6052 || R1052 || Standard promoter, repressed by 434 cI ||
 * BBa_I6054 || R1053 || Standard promoter, repressed by p22 cII ||
 * BBa_I6056 || R1062 || Standard promoter, activated by HSL-bound LuxR ||
 * BBa_I6058 || I0500 || pBad/AraC, arabinose-inducible promoter||
 * BBa_I6044 || R0063 || lux pL from V.fischeri, repressed by HSL-bound LuxR ||
 * BBa_I6046 || R0065 || Hybrid promoter, repressed by lambda cI, activated by HSL-bound LuxR ||
 * BBa_I6048 || R1050 || Standard promoter, repressed by HK022 cI ||
 * BBa_I6050 || R1051 || Standard promoter, repressed by lambda cI ||
 * BBa_I6052 || R1052 || Standard promoter, repressed by 434 cI ||
 * BBa_I6054 || R1053 || Standard promoter, repressed by p22 cII ||
 * BBa_I6056 || R1062 || Standard promoter, activated by HSL-bound LuxR ||
 * BBa_I6058 || I0500 || pBad/AraC, arabinose-inducible promoter||
 * BBa_I6052 || R1052 || Standard promoter, repressed by 434 cI ||
 * BBa_I6054 || R1053 || Standard promoter, repressed by p22 cII ||
 * BBa_I6056 || R1062 || Standard promoter, activated by HSL-bound LuxR ||
 * BBa_I6058 || I0500 || pBad/AraC, arabinose-inducible promoter||
 * BBa_I6056 || R1062 || Standard promoter, activated by HSL-bound LuxR ||
 * BBa_I6058 || I0500 || pBad/AraC, arabinose-inducible promoter||
 * BBa_I6058 || I0500 || pBad/AraC, arabinose-inducible promoter||
 * BBa_I6058 || I0500 || pBad/AraC, arabinose-inducible promoter||