Template:SBB10 ConstructionFiles JeniL sbb34

Construction of Ala6 basic part sbb34 EIPCR Ala6-F/ca2741R on pBjk2741-Bca1144  (2203 bp, BglII) Product is pBjk2741-Ala6   {} Ala6-F EIPCR construction of Alanine-6 part CCATAagatctgcagcagcagcagcagcaGGATCCtaaCTCGCTCCTCagg jl2741R Reverse BglII oligo for Ala6 EIPCR on pBjk2741-Bca1144 CTGAAGTGgaattcatgAGATCTATTGG



JCA Notes

 * Reverse oligo is reverse complemented
 * Should name plasmid as vectorName-partName, not vectorName-shortDescription
 * Due to similarity to other parts, reusing an oligo
 * I've also mutated out the psti for compatibility with other standards

Construction of Ala6 basic part sbb34 EIPCR Ala6-F/tn0002R on pBjk2741-Bca1144  (2219 bp, BglII) Product is pBjk2741-sbb34  {} Ala6-F EIPCR construction of Alanine-6 part CCATAagatctgcTgcCgcagcagcagcaGGATCCtaaCTCGCTCCTCagg tn0002R	Reverse BglII oligo for Tn5 EIPCR CCAATAGATCTcatgaattcCACTTCAG

Part: gatctgcTgcCgcagcagcagcaG