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Kamran Ali

Beta Helix Repeats
Source: E. coli avian APEC genomic DNA You are going to construct a passenger part encoding some beta helix repeats from the Tsh autotransporter. For background, you should read PMID: 15728184. See pubmed nucleotide sequence AF218073 for the DNA. Also check out the annotated protein sequence at AAA24698. You are going to make a BglBricks part out of a small fragment of this gene that encodes: svfngtvnldnqsvlnindifnggiqannstvnissdsavlgnstltstalnlnkganalasqsfvsdgpvnisdaalslnsrpdevshtllpvydyagswnlkgddarlnvgpysmlsgninvqdkgtvtlggegelspdltlqnqmlyslfngyrniwsgslnapdatvsmtdtqwsmngnstagnmklnrtivgfnggtspfttlttdnldavqsafvmrt (which doesn't include the amino acids encoded by the BglBricks polylinker)

HA Tag
Source: pBca1256-K112506  {} This part has already been constructed as a BglBricks basic part in plasmid pBca1256. You will be transferring the part using the Gateway reaction into the assembly vector. To do this, you will react the source plasmid with plasmid pBca1254AK. As background, you should read PMID: 12536251.



This part encodes a passenger protein
After performing the Gateway reaction, your product plasmid will be pBca9495AK-partname. To see what the part looks like in the product vector, look at [[Media:JCA_pBca9495AK-Bca1144.seq | this map]] of the RFP-expressing part Bca1144 in pBca9495AK.