JCA062206-Construction of pSB1A2-J23003

 [pMB1][AmpR][TraJR]+[dblTerm] digest pSB1A2-J01001	(SpeI/AlwNI, Neb2, Large-1905) digest pSB1A2-B0015	(XbaI/AlwNI, Neb2, Small-546) Ligate, Product pSB1A2-J23003 