User:John Szymanski/notebook

= Lab Notebook =

Making Plates
Label plates with date, antibiotic present. Lay out all plates with lids on top. Lift lid, pour 6mm of agar/LB/antibiotic solution, and immediately put lid back on.

Cell Cultures

 * LB solution with ampicillin
 * sterile tubes with loose caps for air
 * 1) Label 2 tubes: Control, Strain of bacteria
 * 2) Sterilize pipette
 * 3) Pipette 3-10 mL of broth in each tube
 * 4) Mark colonies to be cultured on plate
 * 5) Dip scraper in ethanol and flame briefly, then let cool
 * 6) Crack the lid of the plate, scrape off the colony, then immediately inoculate culture
 * 7) incubate @ 37 C overnight with agitation

I made a culture for E.coli strain PRS305

Registry of Standard Biological Parts

 * http://parts.mit.edu

When using biobrick parts, record the following:
 * Part #
 * Name of Part
 * Subparts
 * Location of Physical DNA: well, plate, and plasmid

Parts Used Today
Group 3

a)BBa_J04630 IPTG Inducible promoter with RBS LacI R0010 -> B0034 Plate 1, Well 16F, pSBIAK3