IGEM:Stanford/2009/Notebook/Marys iGEM Notebook/2009/08/03

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mary's SiGEM Project
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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A summary on modeling of Device I
(Updated by Mary on Aug 3, 2009. Please feel free to give any comments or suggestions.)
 * 1.NO->SoxS--SoxR binding:
 * Need to caluculate: steady state percentage of SoxR-SoxS Binding
 * Problem: Probably the dynamic process is too slow (over 4 hours?). Any way to fasten it?
 * 2.SoxS-SoxR binding-->Crt production
 * Not quite clear yet. Need help from Bobby.
 * 3.IPP(+crt)-->B-carotene:
 * Results by far: the largest percentage of B-carotene in all products is 68%, as shown in the table below:
 * [[Image:b-caro distribution.jpg]]
 * Need to find out: the approximate velocity of this process (hopefully not too long...), and how we can adjust the velocity by changing the levels of crtB/E/I/Y.
 * Problem: Bunches of paremeters to be found....@@...We need help...
 * 4.B-carotene(+Blh)-->Retinal(+RalDH)-->RA:
 * Results by far: We found that neither Retinal nor Retinoic Acid is actually degraded in E.coli, thus all B-carotene that is not degraded, will eventually turn into RA.
 * Problem: Still need to find out the appoximate production rate of B-carotene. With it, hopefully we can scan different values of the enzymes in the reaction, namely Blh and RalDH II, to figure out appropriate levels of them that can ensure a comparingly faster process.

Daily Work
Goals

Project

Accomplishments

Problems

Alternative Solutions

Weekly Goals

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