IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/08/02

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BioFilm Track

 * Made 200ml PBS buffer solution for washing biofilm
 * Leaving 96 well plates with staph strain RN4220 for 48hr incubation - Staph strain RN4220 most likely would not form biofilm according to literature references

Started "tube method" to test staph strain 8325 for biofilm formation Content (Heated TSB to dissolve glucose, NaCl. Cooled before adding staph)
 * Time placed in incubator; 4pm (20 hours incubation, 37C, static condition)
 * 10ml TSB in glass tube
 * 5 % w/v glucuose (0.5g)
 * 2 % w/v NaCl (To facilitate biofilm growth)
 * Staph strain 8325 obtained from Lando

Gel Verify
Gel Verify PCR products from last day Protocol: gel verification protocol in Protocol binder (SOP) Gel orientation:
 * Changes: Used 1% agarose gel instead of 0.8%

Machine conditions: 100V, 45 min, 0.5X TBE Buffer Results: Vicki Ma 02:40, 4 August 2010 (EDT)

Restriction Digest(ON)
Protocol: Restriction digest protocol in Protocol binder (SOP)

Vicki Ma 03:08, 3 August 2010 (EDT)
 * DNA: Add 5uL of each A,B,C,D,E,F, psb1A3 (backbone plasmid) [a,b,c,d,e,f]
 * Left ON in 4C fridge on ice

Misc
Poured 2* 400ml Amp plate + 1 * 400ml Chloro plate


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