IGEM:Peking/2007/Switch-Notebook/2007-8-29

miniprep and digestion test
sulA, RS2, RD3-plx012 plasmid is isolated.

Double digestion system:

H buffer 2uL

plasmid 7uL

XhoI/SalI 0.25uL each

ddH2O 10.5uL

colony PCR of LacIq-T
many colonies are right!

re-digestion of CD1-plx012
plasmid 7uL

buffer H 2uL

XhoI/NdeI 0.5uL each

H2O 10uL to a total 20uL system

result: right!

sequencing of LacIq-pcc009
No signal. There must be a problem with either our plasmid or the primer!