User:Fermenter User/Notebook/BROM D3

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM D3

Day 0 (Mar 11 Wed):
           3/11/2009 12:30 Assemble bioreactor     3/11/2009 13:00 Bring media to fermenter room 3/11/2009 13:00 Add 5 ml of Antifoam            3/11/2009 13:13 Start Sterilization (Liq 45 min) 3/11/2009 14:46 Cooling of bioreactor (Temp setup) 3/11/2009 14:46 Purge with 0.5 L/min O2                          3/11/2009 14:51 Start Fermentation Software for Fermenter #6 Record 0:00 3/11/2009 15:00 Connect Acid (500 ml) and Base (400 ml), prime pumps Adjust pH to 7.0 F6: Base: 400 ml - 250 ml = 150 ml (total consumption) 3/11/2009 15:05 Calibrate dO2 probe with 300 rpm, O2 purge for 30 min 3/11/2009 15:05 Set dO2 3/11/2009 15:05 Start pH control 3/11/2009 15:05 Completion of bioreactor set up: Ready for inoculation rpm set to 400 dO2 <40% ---        3/11/2009 00:00 Inoculation: (?? ml) Fermentation 0:00

Day 1 (Mar 12 Thu):
           3/12/2009 (07:30) F6: Acid (480 ml) Base (1500 ml) Increase rpm to 600 Bioreactor picture taken     3/12/2009 (09:10) Sampling Fermentation 00:00 F6: Cell weight (109.8 mg/ml), Activity ( 65%, x100), Activity ( 16%, x1000)            3/12/2009 (13:30) Increase rpm to 800     3/12/2009 (14:00) Sampling Fermentation 00:00 F6: Cell weight ( 191.8 mg/ml), Activity ( 86%, x100), Activity (43 %, x1000)            3/12/2009 (15:00) F6: Base (130 ml)+ 200 ml (330 ml)

Day 2 (Mar 13 Fri):
           3/13/2009 (07:45) F6: Acid (410 ml) Base (310 ml)     3/13/2009 (09:00) Sampling Fermentation 00:00 F6: Cell weight ( 245.6mg/ml), Activity ( 92%, x100), Activity (59 %, x1000) <Frances/Vincent>    3/13/2009 (11:10) Sampling Fermentation 00:00 F6: Cell weight ( 288.2mg/ml), Activity ( 93%, x100), Activity ( 57%, x1000) <Frances/Vincent>    3/13/2009 (14:00) Sampling Fermentation 00:00 F6: Cell weight ( 289.4/ml), Activity ( 95%, x100), Activity ( 64%, x1000) <Frances/Vincent>    3/13/2009 (00:00)     Fermentation 00:00 <Sung-Hye>           3/13/2009 (15:00) Havest and Finish Fermentation F6: Acid (400 ml) Base (300 ml)


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