User:Mradha

Module 3: Yeast

SAGA subunits, S. cerevisiae
Module 1

Protein Modifications

Results of Ligation Reactions

Refactoring M13

Alterations Made

I created three new parts on the Registry of Standard Biological Parts BBa_M31000, M31001, and M31002. M31000 contains the entire DNA sequence from the Hpal site to the BamH1 site. I removed the Hpal site and changed it into another BamH1 site. This allows for this portion of the plasmid to cut up and removed from the rest of the genome. It will also have blunt ends but the ends will anneal to the each other. I removed gene 7 and left gene 8's promoter and gene 9's RBS. In M31001 I added gene X's promoter and RBS to the front of gene X and made it a compact and independent gene. Then in M31002 I changed gene 2 so that gene 10's promoter and RBS were disrupted and unfunctional. This made it so that gene 2 can be manipulated individually compared to gene 10. A few of the problems I ran into were that it was extremely hard to annotate each of the genes without changing the numerical sequence of the genes following it. The parts registry did not make the necessary changes which resulted in me making multiple individual gene parts.

Module 4

20.109:Final Project