IGEM:Harvard/2010/General Protocols/BioBricks

A Biobrick is a piece of DNA flanked by a STANDARD set of restriction sites: EcoRI (E), NotI (N), XbaI (X) upstream to the left; SpeI (S), NotI (N), PstI (P) downstream to the right.


 * Standard Assembly of BioBricks is explained on the Parts Registry Website


 * The sequence of the BioBrick cloning site is: gaattc(E)  gcggccgc(N)   tctag(X)   [BioBrick Part]   actagt(S)   gcggccg(N)  ctgcag(P)


 * E-N-X are referred to as the "BioBrick Prefix" and S-N-P are referred to as the "Biobrick Suffix"


 * The BioBrick part itself must NOT contain any of the standard restriction sites, or else it will be chopped up into pieces during assembly


 * The Biobrick vector we will be using is called V0120, a high-copy plasmid (~300 ng/uL from a mini-prep of a 5 mL culture)