IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/26

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

July 26th, 2010

 * Met with Synthetic Biology Research Group
 * Located supplier and placed order with Julie for E. coli strain with the copper efflux gene knocked out
 * Ran two gels on purified DNA from gel bands
 * Received sequence of copper construct sample from UVA subteam and compared to design sequence to determine if construct was present in PCR product
 * Worked on mathematical modeling
 * Started overnight broth cultures of cotransformed (RFP and GFP) cells in LB carbenicilin and kanamycin, TB carbenicillin and kanamycin, and LB with no antibiotics
 * Measured OD of overnight cultures (above) at 6.5 hours growth
 * Started overnight broth cultures of TOP10, EGFP, ACGFP, and DSRED cells for spectral unmixing
 * Uploaded journal entries onto iGEM WIKI
 * Autoclaved glassware, supplies, and biohazard waste


 * }