IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/05/Zhangs

Transfer the transformed bacteria into the LB and incubate in shaker. 30 samples in total, all for RBS + CI + 1-23L samples, 5 samples for each RBS (1-11N. 1-2I, 1-5J, 1-1H, 1-5N, 1-2G).

Miniprep for 1-11N + CI + T×5, 1-2I + CI + T×5, 1-5J + CI + T×5, 1-1H + CI + T×5, 1-5N + CI + T×5, 1-5J + CI + T×5, 30 samples in total

Transfer the transformed bacteria into the LB and incubate in shaker. 15 samples in total. 1-2M +CI +1-23L, 1-1J + CI + 1-23L, 1-2K + CI + 1-23L, 5 samples for each.