User:Fermenter User/Notebook/STRY C1

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

STRY C1

Day -1 (Apr 2 Wed):
              4/1/2009 13:20 Bring powder to fermenter room            4/1/2009 11:30 Calibrate pH sensor 4/1/2009 13:30 Make 50-L media 4/1/2009 13:30 Add 10 ml of antifoam 4/1/2009 13:42 Start software 4/1/2009 13:50 Start Sterilization 4/1/2009 15:00 Cool down bioreactor 300 rpm, 50-L/min air 4/1/2009 15:00 Connect Base (1000 ml) and Acid (1000 ml) 4/1/2009 15:00 Start pH control

Day 0 (Mar 24 The):
           4/2/2009 08:00 Connect Acid (1000 ml) and Base (1000 ml) 4/2/2009 08:00 Set pH                          4/2/2009 08:00 Calibrate dO2 probe 4/2/2009 08:00 Set dO2               4/2/2009 10:00  Add antibiotics               4/2/2009 10:00  Fermentation 0:00 Inoculation: (1000 ml)               4/2/2009 10:00  Fermentation 00:00 Sampling (F4): OD (used as blank)               4/2/2009 12:10  Fermentation 02:10 Sampling (F4): OD (not measured)            4/2/2009 12:20  Fermentation 02:10 Change temperature               4/2/2009 13:30  Fermentation 03:30 Sampling (F4): OD (0.4)            4/2/2009 14:30  Add inducer               4/2/2009 00:00  Sampling Fermentation 00:00 F4: OD (??)

Day 1 (Apr 3 Fri):
<Sung-Hye>           4/3/2009 07:00  Acid (980 ml) Base (800 ml) <Sung-Hye>           4/3/2009 09:30  Havest and Finish Fermentation


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