840:153g:Projects/project5/2009/02/12

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Team gNarLy prom-Oters
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Preparation for miniprep

 * Today we analyzed the results of spreading some of the colonies onto LB+Amp plates from Wednesday. All but one of the colonies we picked ended up growing. This means that these colonies are all resistant to ampacilin.
 * On Wednesday, we only had 5 of our LB+Amp plates left, so we borowed 4 from Dr. Schwekendiek. After the overnight incubation, the growth on the 4 borowed plates apear light blue where the cells are the most dence. We're thinking that the most likely cause of this is from the background enzymes.
 * We then sealed our plates and put them in the fridge. We will be making an overnight liquid culture on monday to be ready for tuesday.


 * For this overnight liquid culture, we needed to prepare some LB broth in tubes. This is what we did today.
 * We also read a little bit on the procedure we plan on doing on Tuesday.


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