IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/09/13

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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dspB Track
Plate results:
 * 50uL HR - no colonies
 * 450uL HR - ~20 colonies (most of them red); (A, D - white?; B, C - pink?)
 * 50uL HC - no colonies
 * 450uL NC - no colonies
 * 50uL NR - 3 colonies (2red) (A)
 * 450uL NR - ~20 colonies (~ half of them red) (A, B, C, D)
 * 50uL NC - colonies
 * 450uL NC - 34 colonies (A, B, C, D)

Colony PCR

 * Pick colony from respective plate and place in respective tubes

PCR Cycles:
 * 98C @ 3min
 * Cycle 27x:
 * 98C @ 10 sec
 * 72C @ 30 sec
 * 72C @ 40 sec
 * 72C @ 10 min
 * 10C @ hold

Start: 1:53pm End: 2:57pm

Gel verification colony PCR products
Gel orientation: Results:
 * Protocol: gel verification protocol in Protocol (SOP)
 * Changes: 0.6g agarose/70mL 0.5x TBE buffer
 * Machine conditions: 0.5x TBE buffer, 100V, 50min

Re-Gel verification colony PCR products
Gel orientation: Results:
 * Protocol: gel verification protocol in Protocol (SOP)
 * Changes: 0.6g agarose/70mL 0.5x TBE buffer
 * Machine conditions: 0.5x TBE buffer, 100V, 50min

Began O/N Culture

 * in 37C shaking incubator at 11:30pm


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