Paulsson:Electroporation competent TOP10

Genotype can be found here


 * Electroporation protocol

Batch 12/19/06, labeled with one red dot, 100ul aliquots, made by Per 1.3 x 10(8) cells per ug (transformation efficiency tested with ts-Rep pDNA)FINISHED

Batch 02/11/10, in "Competent Cells II" box, labeled "TOP" in black ink, ~155ul aliquots, made by Per Prepared using 500ml Corning Centrifuge Tubes, final resuspension in larger volume than expected, ~34 aliquots from 1.5L 5.3 x 10(7) ampR cfu per ug pDNA (transformation efficiency tested with 50ul cells and 1ng pCR2.1 pDNA in 1mm cuvette) 3.7 x 10(7) ampR cfu per ug pDNA (transformation efficiency tested with 77ul cells and 1ng pCR2.1 pDNA in 2mm cuvette, both grought to 1ml in SOC after transformation, grown 60min at 37deg, and 100ul plated on LB/Amp)