User:Karmella Haynes/Notebook/BioBrick cloning/2011/01/31

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01/31/11

 * &#x2713; Miniprep digests: p65 w.t.(4), KAH179/176, 180/176, 179/177, 180/177, 179/178, 179/178/MV8 (2 each)

Minipreps > p65 w.t.: X/P > Others: E/ApaLI (Note: KAH176, 177, 178 have 2 ApaLI mCherry sites)

--> Conclusions: ---> mCherry has ApaLI site. Recalculated expected fragments. All make sense except for ~2 kb bands, which are probably partial digests (711+last fragment). Test these by transfection into U2OS.
 * p65 cloning worked
 * Unsure about miR sensor assemblies. Largest band shorter than expected and there are many additional bands. Will check restriction sites and send circled clones off for sequencing.


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