YPD and YPAD medium


 * Omit agar for liquid culture.
 * Agar plates are at 2% rather than the 1.5% usual in bacterial work.
 * Dry the plates 2-3 days at RT before storing at 4C for several months.
 * Autoclave components for a maximun of 15 minutes at 121C.
 * For large volumes, autoclave salts, glucose and agar separately.

YPD (YEPD) medium
A complex medium for routine growth. Available premixed as Difco 0428-17.


 * per liter
 * Bacto yeast extract (Difco 0127-17) (1%)  10g
 * Bacto peptone (Difco 0118-17) (2%)  20g
 * Glucose (2%) 20g
 * Bacto agar (Difco 0140-01) (2%) 20g

YPAD medium
A complex medium to inhibit reversion of the ade1 and ade2 mutations. As above, with added adenine. Inhibits red coloration in ade strains such as AB1380.


 * Bacto yeast extract (Difco 0127-17) (1%)  10g
 * Bacto peptone (Difco 0118-17) (2%)  20g
 * Glucose (2%) 20g
 * Bacto agar (Difco 0140-01) (2%) 20g
 * Adenine sulfate (.004%) 40mg

YPG (YPG or YEP-glycerol) medium
A complex medium containing non-fermentable carbon source (glycerol) that does not allow growth of &rho;- or pet mutants.


 * per liter
 * Bacto yeast extract (Difco 0127-17) (1%)  10g
 * Bacto peptone (Difco 0118-17) (2%)  20g
 * Glycerol (3% v/v) 30 ml
 * Bacto agar (Difco 0140-01) (2%) 20g