User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/04

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08/04/10

 * &#x2713; Cultures/ minipreps: KAH160-170 (2 each, 2 mL)
 * &#x2713; Sequencing order: KAH015 (TetO-FUW-Oct4 + microMCS)

Minipreps > Check with E/P digests

Sequencing order > KAH015 (TetO-FUW-Oct4 + microMCS) --> Forward = KAH015seq F --> Reverse = KAH015seq R


 * 1) KAH015-1 F: low quality seq (early termination)
 * 2) KAH015-2 F: low quality seq (high background)
 * 3) KAH015-3 F: 2x microMCS inserted in reverse orientation
 * 4) KAH015-4 F: good
 * 5) KAH015-1 R: low quality seq (early termination)
 * 6) KAH015-2 R: low quality seq (high background)
 * 7) KAH015-3 R: 2x microMCS inserted in reverse orientation
 * 8) KAH015-4 R: good

--> Keep and use KAH015-4


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