User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/06/24

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 * width="500pt" align="center" style="background-color: #EEE"|[[Image:owwnotebook_icon.png|48px]] |T7 RNAP transcription of rRNA
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 * 1) Induce cells if necessary and lyse by the end of the day (or the next morning)
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 * 1) Induce cells if necessary and lyse by the end of the day (or the next morning)

Primer design information
>wt TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA >mutant TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCATTGTGGTTA

wt16sProbe1 wt16sProbe2
 * 5'-GGT TGG ATC ACC TCC-3' 49
 * 5'-GTT GGA TCA CCT CCT TA-3' 48 - ordered this

o16sProbe1 o16sProbe2
 * 5'-GTT GGA TCA TTG TGG-3' 44
 * 5'-GTT GGA TCA TTG TGG TTA-3' 47 - ordered this


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