IGEM:IMPERIAL/2009/phases of project

Time regulation
1. NYMU 2008 Project

2. Genetic toggle switch

3. Timer from Dr. Karen's talk

why encapsulate?

 * enhances cell resistance to freezing and freeze-drying (for storage)
 * added convenience and reduced packaging costs
 * longer stability and viability during storage

natural encapsulation
encapsulation occurs naturally when bacteria trapped in own exo-secretions many lactobacilli secrete exo-polysaccharides, but most are insufficient to cover fully

Recent review on probiotic encapsulation methods

Phase 3: Plasmid deletion

 * Bactoblood Project from Berkly 2007 - Plasmid Deletion and Freeze Drying Bacteria
 * Programmed Cell Death - Use of anti-toxins and toxins to control cell death
 * Cell Death mechanism by KU Leaven 2008 iGEM
 * Articles about using restriction enzymes to lyse the host chromosome
 * Class of Bacterial toxin