Smolke:Protocols/dNTP Mixture

Summary
dNTP mixture: 40 mM NTPs, 10 mM each. Makes 1.25mL for a total of 25, 50 &mu;L aliquots.

Ingredients

 * 100 mM ATP
 * 100 mM CTP
 * 100 mM GTP
 * 100 mM TTP
 * dH2O, preferably DEPC treated

Protocol

 * 1) Add 125 &mu;L each NTP.
 * 2) Add 750 &mu;L dH2O.
 * Mix, Vortex.
 * 1) Aliquot out 50 &mu;L of mixture into 0.6 mL microfuge tubes.

Source
Final concentrations based on NEB dNTP pre-mixed solution. Additional questions, ask Midori.