IGEM:Peking/2007/Switch-Notebook/2007-8-1

transformation
lacZa-plx009 gel rescue, lacZa, lacZa-pcc009 precipitation rescue, plx009 and lacZa precipitation rescue

CD2-T sequencing result
wrong! We have to re-PCR CD2

RD2-T sequencing result
It showed that 1 and 5 colony is right, miniprep using Kit

Miniprep of T1-T10
SacII and BSA, T buffer to digest the plasmid to see if it is right

Miniprep of plx007 1-6 and plx008 1-6
XhoI cannot cut it. We have to reconsider our testing plan

Plasmid isolation using Kit
RD2-pGEMT is isolated and digested with PstI and H buffer It is all right and send to storage.

CD2-pGEMT, lacZa-plx009 colony PCR
The band seems in wrong place. RePCR tomorrow.

PCR T1TE and T1T2
rescue by cuting the gel after electrophoresis.