BCH072506-Construction of pJ23006-J23036

[TT] E [Ptet] X [RFP] S P + X [Lock3h] S P Klenow extend KB022/KB019; digest (XbaI/PstI, Neb2, small frag cleanup) Paste into pJ23006 (XbaI/PstI, Neb2, large-2256bp) Product is pJ23006-J23036