User:Jonathan Cline/Notebook/Melaminometer/Meetings

Abbreviations:


 * JC = jcline
 * NYMU = National Yang Ming University, Taipei, Taiwan
 * Chuan-Hsiung Chang's lab
 * Chih-Hsien Yang OWW User:Blent
 * MP = Meredith L. Patterson

2008-11-04 JC+NYMU
Attendees:
 * JC
 * NYMU

Conf call Summary


 * Introductions
 * Capabilities, existing NYMU technology & licensing
 * product goal discussion
 * OWW open source project


 * set next meeting date/time 2008-11-18

2008-11-11 JC+SG

 * Discussion of competitive methods for detection.
 * ELISA
 * You make a serial dilution and then get a good estimate of your antigen concentration by identifying the dilution at which you can first identify a color change.

2008-11-18 JC+NYMU
Attendees:
 * JC
 * NYMU

Conf call Summary


 * postponed due to travel.
 * set next meeting date/time 2008-11-25

2008-11-25 JC+NYMU
Attendees:
 * JC
 * NYMU

Conf call Summary


 * Introductions
 * NYMU team assignments
 * Discuss NYMU presentation & JC OWW research
 * [[Image:Melamine Sensor Project 20081125 am11.pptx]]


 * Custom protein
 * Example, combine light sensing protein in cyanobacteria with melamine receptor for detection
 * Chassis
 * Idea, use ADP1 as it has been proposed before
 * Set next steps:
 * NYMU Want to get current market ELISA kit for evaluation & calibration in lab
 * Need to scope where to get other materials
 * NYMU Need to scope designing enzymes into e.coli
 * JC to investigate creating biobricks from known seqs in research
 * Need to investigate noise issues for detection in sample
 * Need to investigate regulating enzymes


 * Set next meeting date/time 2008-12-04 9AM (TAIPEI time)

JC Wiki Updates

 * Created rough outline schedule on wiki
 * created meeting notes on wiki

2008-12-4 JC+NYMU
Attendees:
 * JC
 * NYMU
 * Chih-Hsien Yang

Conf call Summary


 * Introductions
 * Discuss team work method
 * All data will be on wiki.
 * No secondary mailing list or doc sharing site at this time. All docs uploaded to wiki.
 * Discuss Schedule
 * Discuss current work
 * ELISA kit study
 * Assumed to be more costly than synbio solution, since kit needs purified enzymes in the kit
 * Melaminometer must be better performance than ELISA kit to be successful
 * Enzymatic method study
 * Contradiction between biocyc.org and papers and multiple action from triA
 * Reproduce previous experimental result from 2001 paper; verify triA activity
 * NYMU will request materials from author
 * Antibody method study
 * Need to model this for melamine; find homologues
 * 12227, 12228 from 2001-paper.
 * May be more successful method than pH detection
 * Single plasmid should be possible for the multiple genes; will make fabrication easier
 * Next week's work
 * 1) JC do patent search for related products
 * 2) NYMU investigate ELISA kit; two examples:
 * 3) http://www.gentaur.com/melamine_elisa_test_kit.htm
 * 4) http://www.romerlabs.com/pdts_kits.html
 * 5) NYMU define materials required for testing (positive, negative controls)
 * 6) NYMU compare homologues of melamine deaminase
 * 7) MP compare homologues of enzymes
 * 8) NYMU contact author of 2001 for strain availability

2008-12-11 JC+NYMU+MP

 * alternative methods (in vivo and in vitro) - CHC
 * ELISA
 * MEMS (e.g. electronic nose, lab-on-chip)
 * synthetic biology
 * or combinations
 * agenda next week
 * discussion of designs
 * assessment and evaluation of detection methods
 * make a action plan before the end of Dec. 2008

2008-12-18 JC+NYMU+MP

 * Suggestion to recognize physical structure of melamine
 * size
 * charge
 * hydrophobicity
 * Need to duplicate same design method for cyanuric acid too
 * Need to convert thresholds from mg to molar depending on sample
 * Using ph sensor - cells may balance ph leading to detection problems
 * Discussion of using multiple methods together


 * JC suggests NYMU call TW food testing agencies to get their opinion on if/when/how they plan to use ELISA testing - none of the agencies are listed as using ELISA; why?


 * Next mtg set for Dec 25 9am-TW for JC+Blent
 * Next mtg set for Dec 31 9am-TW for JC+NYMU

2008-12-25 JC+Blent

 * Ordering of pure melamine sample:
 * Sigma CAS number: |ALDRICH&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC 108-78-1 (1,400 NTD)
 * Evaluation of pH sensor in higher pH condition

2008-12-30 JC+blent

 * skipped

2009-01-08 JC+NYMU

 * idea #1
 * idea #2
 * modeling and preliminary experiments
 * sensitivity
 * specificity
 * reaction time

2009-01-15 JC+NYMU

 * where is the market?
 * to have marketing and social impact
 * shorter reaction time (less than 20 min)
 * better sensitivity (outcompete with ELISA)
 * less expensive (less than 5 dollars)
 * to have academic impact
 * novel and new method