WangLab:Alignment of Microscopy

Alignment of Microscopy

 * 1) Align the lights, adjust the knob in the light house to position the light in the center.
 * 2) Align the field.
 * 3) *switch eyepiece to O.
 * 4) *close down the field aperture.
 * 5) *Adjust the knobs to center the field in the center and enlarge it to see whether it can fulfill the field.
 * 6) Align the phase ring.
 * 7) *switch the eyepiece to Boucher, adjust the knob in the eyepiece until see a sharp and clear image.
 * 8) *use hand to manually adjust the phase ring until it is centered.
 * 9) *Switch the eyepiece to O again, adjust the condenser height.
 * 10) *Focus

Taking Images with time lapse video function:

 * 1) Turn on microscopy, slide the filter to direct the light to a correct camera, turn on controllers, monitor
 * 2) In the mac computer, go to scion image->special->load macros->time lapse video.txt
 * 3) Special->start capture->select regions of interest (alt-A for the whole image)->special->save->select numbers of frames based on calculation->select the time intervals->save