User:Karmella Haynes/Notebook/miR Trigger/2011/02/24

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02/25/11

 * &#x2713; JDS33-47: split 1:10 and set up 2 6-well transfection plates (ab-free medium)
 * &#x2713; U2OS plain transfections: check expression of clones

U2OS plain transfection
 * 1) KAH179/176/MV8: Target +UAS/ Repressor ---
 * 2) KAH180/176/MV8: Target ---/ Repressor ---
 * 3) KAH179/177/MV8: Target +UAS/ Repressor m-target
 * 4) KAH180/177/MV8: Target ---/ Repressor m-target
 * 5) KAH179/178/MV8: Target +UAS/ Repressor s-target
 * 6) KAH180/178/MV8: Target ---/ Repressor s-target

> Construct #4 appears to be the most promising: good survival and expression of both CFP (Target) and RFP (Repressor) as expected > Tomorrow, reduce zeo dosage to 200 μg/mL


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