CRI nanodrop users guide

General Info
This SOP is a how to use guide for the analysis of DNA, RNA and Primer concentration using the Nanodrop ND-100.

Materials

 * P2 Single Channel Pipette
 * ART 10 Reach sterile pipette tips
 * Sterile de-ionised water
 * Blue paper towel
 * Samples for concentration testing.
 * Nanodrop ND-100 Spectrophotometer connected to PC with correct Software.

Procedure
For DNA / Primer concentrations:


 * 1) Log onto Nanodrop PC using your user name and password.
 * 2) Open Nanodrop 3.0.0 by double clicking on the Nanodrop 3.0.0 icon on the desktop.
 * 3) Click on “Nucleic Acid Measurement” button.
 * 4) Using the blue paper towel, gently wipe both parts of the electrode.
 * 5) Load a fresh tip onto the pipette as specified in JGLSOP32
 * 6) Set the dispensing volume to 1µl
 * 7) Draw up 1µl of sterile de-ionised water and dispense onto the bottom electrode of the Nanodrop.
 * 8) Eject tip into yellow hazardous container after use.
 * 9) Lower the top arm down into position.
 * 10) Click “ok” button to initialize the spectrophotometer.
 * 11) Once complete, raise the arm and wipe both electrodes again with blue roll.
 * 12) Repeat steps 6 to 9 again.
 * 13) Click “Blank” button.
 * 14) Clean both electrodes again, using a fresh piece of blue paper towel.
 * 15) Ensure Sample Type box is set to DNA-50 for all DNA concentrations. If not, change using drop down menu. (For Primer concentrations, select “other” and change constant to “33”)
 * 16) Using a fresh tip, load pipette with sample (1µl) and dispense onto electrodes.
 * 17) Lower top arm.
 * 18) Click “Measure” button (located at the top left hand corner of the screen)
 * 19) Record Value (ng/µl) displayed at the bottom right hand side of the Nanodrop user screen.
 * 20) Raise arm and clean electrodes using fresh piece of blue paper towel.
 * 21) Repeat steps 16 to 20 until the required number of samples have been analysed.
 * 22) Click on “Exit” button to leave program.
 * 23) Click “Exit” again to return to the desktop.