Template:SBB10 ConstructionFiles MaziarB sbb19

Construction of FokI Cleavage Domain basic part sbb19 PCR mb0001/mb0003 on BBa_K157000 (504 bp, gp = A) PCR mb0002/mb0004 on BBa_K157000 (143 bp, gp = B) --- PCR mb0001/mb0004 on A+B          (622 bp, EcoRI/BamHI) Digest pBcajk2741-Bca1144         (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb19	      {T  cataaaaccaatTgcaatggcgccg mb0003 Reverse point mutation A-->T  cggcgccattgcAattggttttatg mb0004 Reverse BamHI  gtacgGGATCCTTAaaaattgatctccccattg



JCA Notes

 * Fixed wiki link
 * Prefix should be ATG not GCT
 * Changed template name to partName
 * Construction file is correct otherwise
 * Changing some oligos for sharing with another part

Construction of FokI Cleavage Domain basic part sbb19 PCR fk0001/fk0003 on BBa_K243000 (504 bp, gp = A) PCR fk0004/mb0002 on BBa_K243000 (144 bp, gp = B) --- PCR fk0001/mb0002 on A+B          (622 bp, EcoRI/BamHI) Digest pBjk2741-Bca1144         (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb19	      {<FokI+!} --- fk0001  Forward EcoRI and BglII for FokI ccaaaGAATTCatgAGATCTCAGCTGGTTaaatctgaactggaggag fk0004  (F)Base Substitution FokI ccataaaaccaatTgcaatggcgccg fk0003  (R)Base Substitution FokI cggcgccattgcAattggttttatgg mb0002 Reverse BamHI gtacgGGATCCTTAaaaattgatctccccattg