Muratore:Protocols/Transformation

Bacterial Transformation

 * 1) 200 μL chemically- competent E. coli + 5 μL DNA
 * 2) 30 min on ice
 * 3) 30 sec @ 42°C
 * 4) 2 min on ice
 * 5) add 800 μL  SOC
 * 6) 45 min @ 37°C
 * 7) spread 200 μL on rich-media plates
 * 8) &rarr; 37°C O/N (inverted)
 * 9) store remainder @ 4°C
 * 10) * spread remaining 800 μL on fresh plates if poor/no growth observed
 * 11) * otherwise, discard remainder after ~24h