Shreffler:Notebook/Staff Meetings/2011/03/15

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Agenda Items

 * EoE
 * Early Risk factors (recruiting, nothing for lab)
 * Biomarkers (recruiting, n=12)
 * EDN assays: will follow up with FL re: his experience; SK will tentatively plan to use assay with wider dynamic range
 * will not attempt to measure eotaxin from saliva at this time
 * AM will order reagents for 16S cloning and plan in early April to pilot this with saliva pellet samples


 * Basophil
 * APC project
 * SP showed data on DR kinetics by flow comparing surface and intracellular expression at 20' and 18h from two donors -- one strongly responsive, one weakly
 * supports previous findings and the impression that strong responders upregulate expression of class II, where as weak responders do not
 * DR increases over 18 hours in an IL-3 dependent fashion -- saw this again
 * DR increases in activation dep manner, but decreases from 20' to 18h
 * One new observation was that in the case of the weak donor, activation dependent CD63 upregulation which was not present at 20' was prominent at 18 hours; this has implications for testing -- perhaps esp for activation by weak agonists (e.g. drugs)


 * Allergen Adjuvant
 * we do not expect to hear from proteonomics on new sequence information until end of month
 * zymosan experiment (n=1) shows lack of ALDH1A2 induction by zymosan, strong induction by fraction 3 -- lacks positive control target for zymosan (e.g. IL-10) to determine whether iDC are unresponsive to zymosan generally, or simply this pathway is not stimulated


 * PM Baso
 * AM repeated the anergy experiment, including a 90' rest between stim 1 and 2. Clear desensitization, but some 'carryover', specifically with Derp2 stim conditions (perhaps higher affinity)
 * SP notes that we cannot distinguish in these experiments between insufficient suppression of degran in Ca free conditions versus delayed degranulation occurring upon shift into Ca

**: Note nec format for pcr data needs to be reviewed with AM
 * JAX
 * updated scripts for JAX to run as data comes in
 * AM to catch up on analysis this month
 * requesting that K at Hopkins adds comment column for lab notes (status?)


 * PM baso
 * AM to finish analysis during AAAAI


 * Carboplatin
 * no changes


 * U19
 * BR to review protocol and plans to begin long term cultures, contact Dutch group
 * will have follow up meeting with JM in ~2 months