Template:SBB10Project-19607

Paulina Tran

sbb21: FokI Cleavage Domain (fok+)
Source: Registry part BBa_K243000 in vector BBa_K157000 Target Sequence: CAGCTGGTTaaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaacctgatggtgccatttataccgttggttccccgatcgattatggcgttatcgttgataccaaagcctatagcgggggttataacctgccaattggtcaggctgatgagatgcagcgttatgtgaaagagaaccagactcgtaacaaacacatcaacccgaacgaatggtggaaagtgtatccgtcaagcgttacagagttcaaattcctgttcgtgagcggccattttaaaggcaactataaagcacagctgacccgtctgaaccataaaaccaatTgcaatggcgccgttctgtcagtagaagagctgctgattggcggtgaaatgatcaaagccgggaccctgacactggaagaagttcgccgtaaattcaacaatggggagatcaatttt Vector: pBjk2741 Short description:  Flavor: { } Family: Restriction Endonuclease Your part exists in the Registry of standard biological parts, but this template sequence differs from what you want by 1 mutation and 3 extra AA at N-term. Take careful note in designing your oligos that you generate the correct product.

sbb08: Tn5 5'TR
Source: Synthetic Target Sequence: GTCGACTGTCTCTTATACACATCTCAACC Vector: pBjk2741 Short description: Tn5 5'TR   Family:  Terminal Repeat