User:Meng Xiao He/Notebook/fall08/2008/11/05

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Transformation results

 * Whatever was spread as X-gal seems to have been toxic
 * Labels were not distinguishable- so don't know which of the TOPO/pER21 cluster is which
 * TOPO cloning much better this time
 * Hundreds on each plate
 * Chem pir-116 are competent it seems
 * pER21 retransformed and grew
 * 2ul pUC19 control from Invitrogen gave hundreds of colonies
 * Ligations w/ pER21 and flanks gave a few colonies each (<15)- 3 picked from each plate into liquid culture


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