User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/21

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05/21/11

 * &#x2713; Minipreps: KAH181, KAH57 (r.t.)
 * &#x2713; Assembly: KAH182

Minipreps > Check with E/P digests

--> Combine samples 3/4

Assemblies
 * 1) KAH182: KAH181/(S/P)/~4644 + polyA2/(X/P)/225

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

---> Add 10 μL to 30 μL DH5α


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