Love:Autoclave:Waste

For sterilizing tissue culture waste. Autoclave room is on the fourth floor of E25, adjacent to the freight elevator. Note: Use clear autoclave-safe bags and not the red biohazardous bags.
 * Set autoclaving conditions:
 * Press Dry button.
 * Sterilization time: 60-120 minutes (small to large).
 * Drying time: 10 minutes.
 * Add 1 liter of water into bag for better sterilization.
 * Loosely tie the waste bag with safety tag. Fill in the information on the tag.
 * Put waste in autoclavable secondary container.
 * Place container in the autoclave.
 * Close the autoclave door, turn the latch, and tighten the wheel.
 * Press Start.
 * Ensure the temperature reaches 121°C before leaving.
 * Enter information into the log.
 * When cycle is complete, make sure the pink strip on the safety tag has become black.
 * Throw the waste in normal trash bin.