User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/06/30

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ABSTRACT

 * PCR of the preffix-RFP-suffix fragment os pSB1T3 plasmid.


 * Today we didn´t observe colonies in the petri dishes of transformed cells with the different ligation reactions, we think this is due to a bad PCR product that is not reacting well with the digested plasmid, and the ligation can´t occur. We had 3 tubes with 3 different extractions of pSB1T3 plasmid so for elucidate which was the best I first run an electrophoresis gel:



According to this gel we decide to use the tube corresponding to the third lane. Pablo and I did 3 PCR reactions with the following proportions:

PCR reaction, we did 2 of this reactions.

PCR negative control

Tomorrow we are going to run an electrophoresis gel to check if the PCR reaction goes well.