Proteinase K

Purpose

 * Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments.

Procurement

 * Proteinase K can be bought at Qiagen or Fermentas

Use

 * Usual stock solution is 20mg/ml in for example TBS
 * Stock solution can be stored at -20°C
 * Optimal temperature is 50 to 55 °C
 * Can be denatured at 95 °C for 10 min
 * The recommended working concentration for Proteinase K is 0.05-1 mg/ml.