User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/14

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Halometabolites from uncultured bacteria
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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TAR cloning

 * Recombined plasmids selected on AMP/TET (100/5ug/ml) grown overnight at 37 in EC100's look good. No background at all and ~150 colonies on recombined sample.
 * 10 clones selected for growth in culture (selection) at 37C with shaking. Will analyze plasmids to see if the recombination worked efficiently.


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