User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/08

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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Gel extraction




1. Excision of the DNA fragments (500 - 800 bp) 2. Put on a filter tip (1,000) in 1.5 ml tube 3. Incubation at -20C for 20 min 4. Centrifusion at 17,900 x g for 5 min 5. Purification of the flowthrough by qiaquick PCR purification kit 6. 50 ul of buffer EB -> 1.5 ng/ul

Addition of emPCR-Compatible Sites and Library Enrichment
load 3.0 ul of sample



Gel extraction by QIAEX II Extraction Kit




Excised gel weight = 180 mg

Eluted with buffer EB 20 ul x 2

Concentration; 1.5 ul


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