User:Amhed Missael Vargas V-/Notebook/Modelling avargas/2010/04/01

{| width="800"
 * style="background-color: #EEE"|[[Image:Avargas_Modelling_Notebook_Unam_Genomics.png|128px]] iGEM 2010
 * style="background-color: #F2F2F2" align="center"|  |Bitacore
 * style="background-color: #F2F2F2" align="center"|  |Bitacore


 * colspan="2"|
 * colspan="2"|

Technic information

 * Luminometers
 * Cellular concetration

Firefly luciferase: An adenilate...

 * Specified reactions, constants and Kms
 * Possibles mutants see Gomi, et al.
 * Quantum Yield variable torugh the medium, see Gomi, et. al.
 * Co-A tends to stabilize the decay of the luminiscense.
 * Many inhibitors, like AMP.

Photographic detection ...

 * A rapid decay (they not used Co-A)
 * E. coli anaerobic?

Oxyluciferin, a luminiscense product of the firefly...

 * LRE stable
 * Competition oxy-luciferin

BCR...

 * System of regenerate ATP from the AMP, product of the luciferase reaction.


 * }