User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/10

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Start stimulation D9, Stop stimulation D12
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * GAPDH western blot VASP blot (1:2000) --> Ht4 cells unsuccesfull, repeat 1:500 dilution --> still unsuccesfull
 * Start stimulation D9
 * Split other D9 hTERT
 * Stop stimulation D12
 * Split Ht4
 * collect cells in end volume 21 mL
 * Take 7 mL of cell suspension and dilute in 53 mL
 * Divide over 4 flask (15 mL each)

Results

 * Viability staining
 * PICTURE --> ANOUK

Conclusion

 * ✅ Ow..

Same topics

 * Splitting cells 10Feb2010
 * Splitting cells 09March2010


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