Muratore:Materials/Plasmids/pTXB1His

Information
pTXB1 with a His8 tag after the chitin binding domain.

See User:Kathryn_Muratore/Notebook/AU_CHEM-570_lab_prep/2011/06/13 to User:Kathryn_Muratore/Notebook/AU_CHEM-570_lab_prep/2011/06/15 for cloning details.

Sequence
1 AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATAAC TACGTCAGGT 51 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 101 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 151 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 201 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 251 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 301 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 351 CGCCCCGAAG AACGTTCTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 401 TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 451 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 501 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 551 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 601 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 651 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 701 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 751 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 801 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 851 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 901 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT 951 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 1001 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 1051 TTTACTCATA TATACTTTAG ATTGATTTAC CCCGGTTGAT AATCAGAAAA 1101 GCCCCAAAAA CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT 1151 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT 1201 TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC 1251 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA 1301 AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA 1351 TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT 1401 GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT 1451 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA 1501 AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA 1551 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTAAAAGGAT 1601 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 1651 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 1701 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 1751 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 1801 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC 1851 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 1901 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 1951 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 2001 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 2051 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 2101 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 2151 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 2201 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 2251 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 2301 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 2351 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT 2401 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG 2451 TCAGTGAGCG AGGAAGCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 2501 CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA 2551 TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT 2601 TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG 2651 CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC 2701 TGCGGTAAAG CTCATCAGCG TGGTCGTGCA GCGATTCACA GATGTCTGCC 2751 TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG 2801 GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA 2851 CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG 2901 ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC 2951 CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC 3001 GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCCGAA CGCCAGCAAG 3051 ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC 3101 GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT 3151 GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG 3201 CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC 3251 TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA 3301 TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC 3351 ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC 3401 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 3451 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC 3501 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT 3551 TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC 3601 CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA 3651 TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA 3701 CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA 3751 TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG 3801 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG 3851 CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA 3901 CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG 3951 CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT 4001 CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA 4051 AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG 4101 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA 4151 GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC 4201 GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC 4251 GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA 4301 TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG 4351 TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA 4401 AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC 4451 CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC 4501 CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT 4551 GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC 4601 TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC 4651 CGCAAGGAAT GGTGCATGCC GGCATGCCGC CCTTTCGTCT TCAAGAATTA 4701 ATTCCCAATT CCCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC 4751 TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG 4801 GACAAATCCG CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG 4851 GGTGGCGGGC AGGACGCCCG CCATAAACTG CCAGGAATTA ATTCCCCAGG 4901 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT 4951 CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG 5001 CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC 5051 CCGCCATAAA CTGCCAGGAA TTAATTCCCC AGGCATCAAA TAAAACGAAA 5101 GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA 5151 ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG 5201 AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG 5251 GAATTAATTC CCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT 5301 GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG 5351 ACAAATCCGC CGGGAGCGGA TTTGAACGTT GCGAAGCAAC GGCCCGGAGG 5401 GTGGCGGGCA GGACGCCCGC CATAAACTGC CAGGAATTAA TTCCCCAGGC 5451 ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC 5501 TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC 5551 GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC 5601 CGCCATAAAC TGCCAGGAAT TGGGGATCGG AATTAATTCC CGGTTTAAAC 5651 CGGGGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT 5701 GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG 5751 GAGATATACA TATGGCTAGC TCGCGAGTCG ACGGCGGCCG CGAATTCCTC 5801 GAGGGCTCTT CCTGCATCAC GGGAGATGCA CTAGTTGCCC TACCCGAGGG 5851 CGAGTCGGTA CGCATCGCCG ACATCGTGCC GGGTGCGCGG CCCAACAGTG 5901 ACAACGCCAT CGACCTGAAA GTCCTTGACC GGCATGGCAA TCCCGTGCTC 5951 GCCGACCGGC TGTTCCACTC CGGCGAGCAT CCGGTGTACA CGGTGCGTAC 6001 GGTCGAAGGT CTGCGTGTGA CGGGCACCGC GAACCACCCG TTGTTGTGTT 6051 TGGTCGACGT CGCCGGGGTG CCGACCCTGC TGTGGAAGCT GATCGACGAA 6101 ATCAAGCCGG GCGATTACGC GGTGATTCAA CGCAGCGCAT TCAGCGTCGA 6151 CTGTGCAGGT TTTGCCCGCG GAAAACCCGA ATTTGCGCCC ACAACCTACA 6201 CAGTCGGCGT CCCTGGACTG GTGCGTTTCT TGGAAGCACA CCACCGAGAC 6251 CCGGACGCCC AAGCTATCGC CGACGAGCTG ACCGACGGGC GGTTCTACTA 6301 CGCGAAAGTC GCCAGTGTCA CCGACGCCGG CGTGCAGCCG GTGTATAGCC 6351 TTCGTGTCGA CACGGCAGAC CACGCGTTTA TCACGAACGG GTTCGTCAGC 6401 CACGCTACTG GCCTCACCGG TCTGAACTCA GGCCTCACGA CAAATCCTGG 6451 TGTATCCGCT TGGCAGGTCA ACACAGCTTA TACTGCGGGA CAATTGGTCA 6501 CATATAACGG CAAGACGTAT AAATGTTTGC AGCCCCACAC CTCCTTGGCA 6551 GGATGGGAAC CATCCAACGT TCCTGCCTTG TGGCAGCTTC AACACCACCA 6601 TCATCATCAC CACCACTGAC TGCAGGAAGG GGATCCGGCT GCTAACAAAG 6651 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA 6701 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG