User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/06

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

07/06/11

 * Cultures/minipreps: KAH183-177/MV8 (4)
 * Sequencing order: KAH183-177/MV8 (4), and Daniel's miR-344 sensors

Minipreps > Check via sequencing

--> Primers: f = DB122, r = BGHR
 * 1) KH11, 183-177MV8-1, f: 7/7/11 no priming
 * 2) KH11, 183-177MV8-1, r: 7/7/11 no priming
 * 3) KH11, 183-177MV8-2, f: 7/7/11 smear; 7/8/11 re-run, good
 * 4) KH11, 183-177MV8-2, r: 7/7/11 smear; 7/8/11 re-run, good
 * 5) KH11, 183-177MV8-3, f: 7/7/11 poor quality
 * 6) KH11, 183-177MV8-3, r: 7/7/11 no priming
 * 7) KH11, 183-177MV8-4, f: 7/7/11 smear; 7/8/11 re-run, good
 * 8) KH11, 183-177MV8-4, r: 7/7/11 smear; 7/8/11 re-run, good


 * }