IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/08/14/Rencheng Gao

11:00 Miniprep: pcrS1, 3~6, plaS 1~6

14:00 Double enzyme digestion (pla2, pcr2, pcrS6, plaS3, plaS4)

18:30 PCR: pla1+SupD, pcr1+SupD

00:10 Double enzyme digestion: plaS4, pcrS6(back vector)