User:Karmella Haynes/Notebook/Polycomb project/2011/02/20

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mm/dd/yy

 * Outline of experiments/ tasks

Task 1 > Line item 1

Lipo transfection > Details

> Add Lipo to 250 μL Opti-MEM --> R.T/ 5 min. > Add DNA to 250 μL Opti-MEM > Add DNA mix to Lipo mix --> R.T./ 20 min. > Remove 500 μL medium from each well; Add 500 μL complexes to each well (4 ml med. each); Grow cells at 37&deg;C > Refresh medium after 5 hours (ab-free)

Fugene transfection > Details

> Master mixes (4, x6): 10.8 μL Fugene + 109.2 μL Opti-MEM --> R.T/ 5 min. > Add 6x vol DNA --> R.T./ 20 min. > Add 20 μL complexes to each well (1 ml med. each); Grow cells at 37&deg;C overnight


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