IGEM:IMPERIAL/2006/LabCalendar/2006-9-3

 TO DO: *Testing of T9002, J37016, J37020, S01656, J37015, J37015RS *Mini & maxiprep of J37022 and J37025 (CHECK whether ligations were successful) *Ligation for the final predator: J37019 + J37025 & subsequent electroporation *Repeat ligation of J37018 ? (has not been completed & successful yet !)

Maxipreps

 * Maxipreped J37022(1) (colonies a&b), J37025(3) (colonies b&c), J27025(4) (colonies b&c).
 * Unable to do all 11 maxis due to lack of maxiprep supplies.

Minipreps

 * Minipreped J37022(1) (colonies a,b,c,d), J37025(3) (colonies a,b,c,d), J27025(4) (colonies a,b,c,d)



Ligations

 * Re-ligating J37018
 * Ligating J37036 (one of the final ones...)

Culturing

 * Cultured up 2ml of T9002, J37015, J37015RS, J37016 and J37020 from frozen stick
 * Cultured up 100ml of aiiA from Dr.Frey in LB medium for maxiprep
 * Cultured up 2ml of 4 different J37022 colonies
 * Cultured up 2ml of 4 different J37025 colonies
 * Cultured up 2ml of S01656 + AHL + IPTG
 * Cultured up 2ml of S01656 + AHL - IPTG
 * Cultured up 2ml of 1M as a control + AHL
 * Cultured up 3 colonies of S01656 in Rosetta cells in 5ml of LB
 * Cultured up 3 colonies of S01656 in BL21 cells in 5ml of LB

Electroporation

 * Had a bit of problem with continual blowing up, so used Kirsten's machine
 * Successfully electroporated J37031 + 9G
 * Unsuccessful for J37025 + J37019 (repeat tomorrow morning