Light my XYZ: Material from Ed


 * isolation of PR called 31A08 from BAC. This paper includes abs spectra from membrane preps and pH measurements of media [[Image:Bactrhod Science00.pdf]] Also link for 31A08 sequence at NCBI accession number AF279106 []
 * characterization of two other PRs called HOT 75m and HOT 0m that were gifts from Chon in Ed's lab on 080805 [[Image:Proteorhodops Nature01.pdf]] NCBI accession number AF349981 for HOT 75m4 [] NCBI accession number AF349978 for HOT 0m []
 * point mutation (Q105?) to change pH gradient created in response to light [[Image:Spectraltuning EMBO03.pdf]]
 * example from Archea in which pH gradient created by PR is directly hooked into chemotaxis by making chimera of PR:Che. Not at all clear that this is do-able in E. coli or some other tamed bact. [[Image:PRCheArch biochem03.pdf]]
 * protocol for membrane preps [[Image:PRmembranepreps FEBS97.jpg]]
 * protocol for abs spect in presence of trans-retinal [[Image:RetinalAbsSpect biochem99.pdf]]

Currently have 31A08, HOT 75m and HOT 0m  from Chon in Ed's lab as well as protease deficient strain  UT5600  [] to express them in.

31A08 CDS            complement(50866..51615) /note="light-driven proton pump; has the properties of a                    light-driven proton pump when expressed with retinal in                     Escherichia coli" /codon_start=1 /evidence=experimental /transl_table=11 /product="proteorhodopsin" /protein_id="AAG10475.1" /db_xref="GI:9971913" /translation="MKLLLILGSVIALPTFAAGGGDLDASDYTGVSFWLVTAALLAST                    VFFFVERDRVSAKWKTSLTVSGLVTGIAFWHYMYMRGVWIETGDSPTVFRYIDWLLTV                     PLLICEFYLILAAATNVAGSLFKKLLVGSLVMLVFGYMGEAGIMAAWPAFIIGCLAWV                     YMIYELWAGEGKSACNTASPAVQSAYNTMMYIIIFGWAIYPVGYFTGYLMGDGGSALN                     LNLIYNLADFVNKILFGLIIWNVAVKESSNA"

HOT Om CDS             <1..>753 /codon_start=1 /transl_table=11 /product="proteorhodopsin" /protein_id="AAK30176.1" /db_xref="GI:13560723" /translation="TMGKLLLILGSVIALPTFAAGGGDLDASDYTGVSFWLVTAALLA                    STVFFFVERDRVSAKWKTSLTVSGLVTGIAFWHYMYMRGVWIETGDSPTVFRYIDWLL                     TVPLLICEFYLILAAATNVAAGLFKKLLVGSLVMLVFGYMGEAGIMNAWGAFVIGCLA                     WVYMIYELWAGEGKAACNTASPAVQSAYNTMMYIIIFGWAIYPVGYFTGYLMGDGGSA                     LNLNLIYNLADFVNKILFGLIIWNVAVKESSNA"

HOT 75m4 CDS            <1..>756 /codon_start=1 /transl_table=11 /product="proteorhodopsin" /protein_id="AAK30179.1" /db_xref="GI:13560729" /translation="TMGKLLLILGSAIALPSFAAAGGDLDISDTVGVSFWLVTAGMLA                    ATVFFFVERDQVSAKWKTSLTVSGLITGIAFWHYLYMRGVWIDTGDTPTVFRYIDWLL                     TVPLQVVEFYLILAACTSVAASLFKKLLAGSLVMLGAGFAGEAGLAPVLPAFIIGMAG                     WLYMIYELYMGEGKAAVSTASPAVNSAYNAMMMIIVVGWAIYPAGYAAGYLMGGEGVY                     ASNLNLIYNLADFVNKILFGLIIWNVAVKESSNA"