Freimoser:Recipes:DiluentA

Use
Diluent A is used to dilute NEB T4 ligase to a final concentration of 1U/μl (400x dilution).

Composition

 * 10 mM Tris-HCl
 * 1 mM Dithiothreitol (DTT)
 * 0.1 mM EDTA
 * 50% Glycerol
 * 200 mg/ml BSA
 * 50 mM KCl

Adjust pH to 7.4 (25°C)