BISC 219/2009:Gene Mapping

We would like to thank Dr. Phil Hartman of Texas Christian University for developing this series and providing us with the worms for our crosses.

C. elegans General Information Tools and Techniques Lab 1: Welcome to C. elegans and Mutant Hunt Lab 2: Linkage Test and Backcross Lab 3: Linkage Test and Backcross and Mapping Lab 4: Mapping Part 2 Lab 5: Score!

Schedule of Experiments
{| border="1" ! Lab # !! Dates !! Activity !! Outside lab time ! 1 Examine wild type and mutant worms; practice picking worms; Set up first crosses - Autosomal vs X-linked test Mutant Hunt: search "screen" plates for Dpy worms Pick 2 "wild type" looking worms from each Autosomal vs X-linked cross to new plates Examine phenotypes of autosomal vs X-linked F1 generation Examine mutants from your hunt: If Dpy then cross to N2 males !2 Linkage Test: Cross mutant males by 4 tester strains; Linkage Test: transfer 2 L4's from each cross to new plates (4 plates total) !3 Mapping:Pick 5 Dumpy worms to individual plates Mapping: Pick 3 double mutants (unc and dyp) to separate plates Complementation: Cross your unknown Dpy mutant worms by N2 males !4 Complementation: Pick males from Complementation cross #1 and mate with known Dpy strains Mapping: Pick 3-4 heterozygous males to separate plates (8 males total) and mate with homozygous recessive dpy unc worms Complementation: Examine cross plates for Dpy males !5
 * 9/8 - 9/14
 * Worm Husbandry: view worm videos;
 * 3-4 days later:
 * 9/15 - 9/21
 * Score autosomal vs X-linked cross;
 * 3-4 days later:
 * 9/22 - 9/28
 * Linkage Test:Score for linkage
 * 3-4 days later:
 * 9/29 - 10/5
 * Mapping: cross N2 males with double mutants
 * 3-4 days later:
 * 10/6 - 10-14
 * Mapping: Score and determine map distance between unknown Dpy gene and Unc reference mutation