IGEM:PennState/Labbook/GalenLynch/2007-6-20

June 20th, 2007: 9:45-5:00
 * Troubleshooting experiments
 * bizarre negative control on enzyme assays suggest that some restriction occured
 * all transformations failed
 * Single unifying trait of expirements: amp
 * Laboratory management
 * Set up anhydrous containers for 100% ethanol and kanamycin
 * Need to buy agarose for Dr.Richard's lab
 * Began processing of switching to SYBR green instead of ethidium bromide
 * SYBR is less mutagenic, may have better results, and would use dual purpose (GFP) filter
 * Real time visualization
 * No UV, less damage to DNA and safre to use
 * Pre stain, less toxic, .3 cents per sample
 * Lambda project
 * Looked for part avaliability, explored lambda genome