User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/02/19

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Height calibration
Tangent average based on 10 measurements on the same 1 um bead, fit 0 to 80 sec: -0.0410

0.0410 pix/sec = 0.0410 pix/5nm = 0.0082 pix/nm. So 1 nm = 0.0082 pxl, or

1 pxl = 121.9 nm
Check: 40 steps 10 nm each, or 400 nm displacement

400 nm = 80*0.041 pxl; pxl = 5/0.041 nm = 121.9 nm

CHECK OK

Average SD: 0.061 pxl = 7.47 nm

SD = 7.5 nm
-- 2/22/2010 16:25

Linear Regression for A021910ZCAL09_E:

Y = A + B * X

Parameter	Value	Error

A	15.59389	0.00138

B	-0.04374	3.00803E-5

R	SD	N	P

-0.99819	0.0602	7668	<0.0001

2/22/2010 16:25

Linear Regression for A021910ZCAL10_E:

Y = A + B * X

Parameter	Value	Error

A	15.58053	0.00158

B	-0.04197	3.45575E-5

R	SD	N	P

-0.99741	0.06929	7677	<0.0001

2/22/2010 16:26

Linear Regression for A021910ZCAL08_E:

Y = A + B * X

Parameter	Value	Error

A	15.56832	0.00146

B	-0.04236	2.72846E-5

R	SD	N	P

-0.99829	0.06123	8289	<0.0001

2/22/2010 16:26

Linear Regression for A021910ZCAL07_E:

Y = A + B * X

Parameter	Value	Error

A	15.18403	0.00152

B	-0.0398	3.25563E-5

R	SD	N	P

-0.99739	0.06706	7818	<0.0001

2/22/2010 16:26

Linear Regression for A021910ZCAL06_E:

Y = A + B * X

Parameter	Value	Error

A	15.14745	0.00136

B	-0.04008	2.91786E-5

R	SD	N	P

-0.99794	0.06002	7803	<0.0001

2/22/2010 16:27

Linear Regression for A021910ZCAL05_E:

Y = A + B * X

Parameter	Value	Error

A	15.8854	0.00847

B	-0.04232	1.76769E-4

R	SD	N	P

-0.94234	0.32369	7230	<0.0001

2/22/2010 16:27

Linear Regression for A021910ZCAL04_E:

Y = A + B * X

Parameter	Value	Error

A	15.31804	0.00147

B	-0.04216	3.19662E-5

R	SD	N	P

-0.9978	0.06434	7691	<0.0001

2/22/2010 16:28

Linear Regression for A021910ZCAL03_E:

Y = A + B * X

Parameter	Value	Error

A	15.54347	0.00142

B	-0.04112	3.05693E-5

R	SD	N	P

-0.99785	0.06271	7797	<0.0001

2/22/2010 16:28

Linear Regression for A021910ZCAL02_E:

Y = A + B * X

Parameter	Value	Error

A	15.61833	0.00138

B	-0.04034	3.02747E-5

R	SD	N	P

-0.99785	0.06036	7668	<0.0001

2/22/2010 16:28

Linear Regression for A021910ZCAL01_E:

Y = A + B * X

Parameter	Value	Error

A	15.28446	0.00179

B	-0.03691	3.91125E-5

R	SD	N	P

-0.99571	0.07814	7683	<0.0001


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