IGEM:Peking/2007/Switch-Notebook/2007-9-4

digestion test of T1TE-T1T2-pcc010
This time I use XhoI and KpnI in M buffer

COlony PCR of I7100-434OR, EGFP
buffer 1.5uL

dNTP 1.5uL

primer 0.5uL each

easy Taq 0.1uL

dH2O 11uL

a total 15uL system

Sequencing
Big day! sequencing result back and we got all fragments right except CD3!