IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/29

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Results of transformation with our ligation products

 * Everything grew! Better efficiency with electrop. than with chemical protocol

Single colony PCR to check our transformation
- Single colony PCR : 13μL of SDW+cells, 5μL of Master Mix, 1μL of VF and 1μL of VR (and plate each single colony)

- Load a gel (1.3% agarose) : 5μL of PCR products + 1μL of dye (only 1μL of 100b ladder)

In the death plasmid, the VF-VR size is about 280b.


 * Result : nothing, even no ladder, problem with the gel!

- run again on a e-gel


 * Lane1 :ladder 100bp
 * Lane2 : Pupp colony 1
 * Lane3 : Pupp colony 3
 * Lane4 : Pspac colony 1
 * Lane5 : Pspac colony 3
 * Lane6 : RBS S colony 1
 * Lane7 : RBS S colony 4
 * Lane8 : RBS W colony 1
 * Lane9 : RBS W colony 4
 * Lane10 : agrD colony 1
 * Lane11 : agrD colony 4
 * Lane12 : HyperladderI




 * Result : nothing, just the primers! Problem with our PCR

Plate biobricks from MIT

 * E0840
 * B0014
 * I712007
 * C0012
 * B0015
 * C0061
 * R0063


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