IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct/TestingValidation

 Specifications Design Modelling Implementation Testing/Validation  

Motivations

 * With this testing part, we want to characterize the sensitivity of the prey cell towards the prey molecule (AHL)
 * By testing the part firstly with known AHL concentrations, a transfer curve can be established
 * By referring to the transfer function, this part will serve as an assay for AHL concentrations, which allows us to quantize different AHL concentrations
 * The expression of GFP and thus the level of fluorescence is dependent on the concentration of AHL

Protocol
See T9002 Testing Protocol

Results
Testing Results (processed data) for T9002:
 * 02/09/2006
 * 01/09/2006
 * 31/08/2006
 * 30/08/2006
 * 28/08/2006
 * 27/08/2006
 * 25/08/2006
 * 24/08/2006
 * 23/08/2006
 * 22/08/2006 plate 1 and 22/08/2006 plate 2 (96-well plate was left in shaker overnight)

Parameter extraction

 * Demonstrate how you are extracting the paramters you are interested in from experimental data.
 * Give the results.