UCBigem09-Protocols

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All these protocols in one big list

Polymerase Reactions
Wobble Reaction Cloning by PCR SOEing PCR PCA gene synthesis Colony PCR

Cleanup Reactions
Regular Zymo Cleanup Small-Frag Zymo Cleanup Zymo Gel Purification

Digestions Reactions
EcoRI/BamHI Digest of Wobble Products EcoRI/BamHI Digest of PCR Products BglII Digest of EIPCR Products Ligation of EcoRI/BamHI digests Ligation of EIPCR digests Analytical digests (Mapping) EcoRI/BamHI Digest of vector plasmid

Bacteria Manipulations
Transformation by heat-shock Picking of colonies

Other
LR Gateway Transfers What do I do with my sequencing data? In Vivo Gateway EcoRI/BamHI Transfer

Reaction Guides
SOEing with ProTips Colony PCR

Binding/Display assay protocols
Streptavidin Display Assay - Microscopy - do longer in development Streptavidin Display Assay - TECAN Streptavidin Display Assay - ELESA - no longer in development Streptavidin Display Assay - Photographing Pellets + TECAN Surface-binding Assay Growth Rate Assay Flocculation Assay

Assembly protocols
Assembly Protocol

Miniprep Protocols
Machery-Nagel Miniprep purification of DNA Quiagen Miniprep purification of DNA Quiagen 8-strip Miniprep purification of DNA