User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/12

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01/12/10

 * &#x2713; Minipreps: KAH117(4), 118(1), 119/MV2(1)
 * &#x2713; Cultures: re-pick KAH117/MV2 (4)
 * &#x2713; Assemblies: KAH117, 118/MV2
 * &#x2713; Retransform: KAH94, 95

Minipreps > Check with E/P digests

--> Pick 4 remaining colonies from KAH117/MV2 plate --> Re-assemble KAH117, 118/MV2

Assemblies
 * 1) KAH117/MV2: KAH117/(E/P)/7758 &#x2713; + MV2/(E/P)/4529 &#x2713;
 * 2) KAH118/MV2: KAH118/(E/P)/7930 &#x2713; + "

> Ligations

> Digests (Fermentas FD) --> For cloning KAH94, 95 into new JDS luciferase vectors (miR project)
 * 1) JDS47/(BglII)/7509
 * 2) JDS48/(BglII)/7519

> Measure conc.'s


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