IGEM:Peking/2007/Switch-Notebook/2007-8-19

experimenter: WMC, CDZ

precipitation of RD3, RD2, RS2, CD1
but the electrophoresis shows no visible signs, which means failure

ligation
with GFP-plx007, all use the proportion 4:1

re-digestion of RD3, RD2, RS3, CD1
template: 20uL XhoI 2uL SpeI 2uL H beffer 10uL ddH2O 66uL for a shortage of template, we just use 10-15uL template in each solution