User:Anthony Salvagno/Notebook/Research/2009/03/11

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/Digestion, Ligation, Transformation Retry Day 1
As I mentioned yesterday, the transformation failed probably during the ligation step of the process. Kelly (and Mary Ann) believes that there were too many small fragments compared to ligatable plasmid DNA so there may have been a lot of gDNA self ligation going on. We need to redo the process all over again...

/Official T'form Results
See here for previous comments.


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