BioBricksAssemblyEnzymes

Assembly Standard 10 enzymes Good cutter/no weird side effects Used in the standard EcoRI                  Yes XbaI                   Yes SpeI                   Yes PstI                   Yes NotI                   Yes

Recommended for removal PvuII                  Yes XhoI                   Yes AvrI                   Don't know NheI                   Yes SapI                   Don't know


 * good enzyme, ? not sure how good enzyme is, D has issues that need to be designed around if using

* AatII * AscI ? AvrI 10(future), * BamHI * BstEII * EcoRI: 10, * EcoRV * HindIII * HpaI * KpnI * MluI * MscI * NcoI * NdeI * NheI: 10(future) * NotI: 10, * NsiI * PacI * PstI: 10, * PvuII: 10(future) * SacII * SalI ? SapI: 10(future) * ScaI * SfoI * SmaI * SnaBI * SpeI: 10, * SphI * XbaI: 10, * XhoI: 10(future) * XmnI