User:Korey Griffin

Contact Info





 * Korey Griffin M.S.
 * Santa Cruz Biotechnology, Inc.
 * 2145 Delaware Avenue Santa Cruz, CA 95060
 * 800-457-3801 x129
 * Email me through OpenWetWare

I am currently a technical representative for Santa Cruz Biotechnology, Inc where I communicate real time support for researchers who are coordinating chemical, immunoglobulin, & RNAi experiments with SCBT reagents and labware.

Research time & bench labor is a valuable effort. Having confidence in the reagent quality and all elements of the procedure is an essential component to the intricate process of molecular and cell experiments. Researchers can, and I believe will, improve success through working with vendor technical representatives. The means to obtain, test, provide feedback, and receive follow-up support when necessary brings success.

There are several nuances and variables for each molecular/cell protocols that determine how a result turns out; apparent & straight-forward procedures (ie immuno (western) blotting) contain an extraordinary amount of important steps. Below are resources for research antibody and RNAi studies, that can improve your next experiment. I hope you may find something useful to bring you success in your next experiment.

Methods & Techniques for Immunochemists







 * Antibody Basics
 * Antibody Peptide Neutralization
 * Antibody Protein Coupling (F:P) Ratios
 * Antibody Elution Buffers
 * Antibody Related Solutions & Recipes
 * Antigen Retrieval Technique
 * Chromatin Immunoprecipitation
 * Dual Immunofluorescence Cell Staining
 * Flow Cytometry prep & labeling
 * Immunoprecipitation Guide
 * Immunohistochemistry Paraffin
 * Immunofluorescence Cell Staining
 * In Vitro Kinase Assay
 * Isolate Leukocytes from whole blood
 * Lentivirus Technology
 * Membrane Stripping and Reprobing
 * Nested RT-PCR
 * Nuclear Extract Preparation
 * Puromycin Selection for shRNA
 * shRNA Transfection
 * siRNA Transfection
 * Sodium Orthovanadate Activation
 * Western Blotting Guide
 * Western Blot Optimization
 * Zinc Fixation

Useful Links

 * Cell Lines
 * Cell Marker
 * DOT guidelines
 * ExactAntigen
 * Fluorochrome Table
 * Gene Atlas
 * Human Protein Atlas
 * Oligo Tm

Experience



 * 2007-2009: Save our Shores Sanctuary Steward, Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
 * 2005-2006: Ride-A-Wave volunteer, Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach, whether they are physically, developmentally or economically challenged.
 * 1997: Summer Youth Camp Counselor for Eagle Lake Camp, Pike Forest CO.
 * 1995,1996: Canoe Guide/Interpreter Charles L. Sommers Canoe Base, Ely MN: Groups are outfitted at the Base to travel by canoe through canoe country, the Boundary Waters Canoe Area Wilderness (BWCAW), Ontario's Quetico Provincial Park, and points beyond. The BWCAW and Quetico together form a roadless area of about a million acres with deep glacial lakes and densely forested cover.

Education & Publication

 * 2000, MS Biochemistry, University of Missouri-Columbia, Advisor: Gary Weisman Ph.D.
 * 1997, BS, University of Missouri-Columbia

Publications
J Biol Chem. 2004 Feb 27;279(9):8212-8. Epub 2003 Dec 11. Src homology 3 binding sites in the P2Y2 nucleotide receptor interact with Src and regulate activities of Src, proline-rich tyrosine kinase 2, and growth factor receptors. Liu J, Liao Z, Camden J, Griffin KD, Garrad RC, Santiago-Pérez LI, González FA, Seye CI, Weisman GA, Erb L.

J Cell Biol. 2001 Apr 30;153(3):491-501. An RGD sequence in the P2Y(2) receptor interacts with alpha(V)beta(3) integrins and is required for G(o)-mediated signal transduction. Erb L, Liu J, Ockerhausen J, Kong Q, Garrad RC, Griffin K, Neal C, Krugh B, Santiago-Pérez LI, González FA, Gresham HD, Turner JT, Weisman GA.