IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/28

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6/28/10
 11:00 am: 

PCR of backbones:

10x Buffer   2 ul

dNTPs  .5 ul

Template 1ul

Polymerase .2 ul

Primer F 1 ul

Primer R 1ul

diH2O 14.3 ul

Total: 20 ul

Template: pSB1A2, pSB1C3, pSB1T3, pSB1AC3-2, pSB1AK3 Psb1AT3.

Negative Controls: 

1. no template, 1 ul water instead

2. For each sample, no polymerase, .2 water instead.

Program: iGEM 2Kb

11:35 am

Diluted primers VF2 and VR to 20 um,

PCR:

used 100ng template for each reaction

Program: iGEM 2Kb

1:15 PM

digestion for Steve

RFP

Lock1

key1 pBAD

2:30 PM gel GRAB PICTURE FROM COMPUTER

 3:30 PM  GRAB PICTURE FROM COMPUTER

Gel of Backbones

Top half of gel

Bottom half of gel


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