Tk:video protocols


 * Making plates / Media
 * antibiotics
 * weighing
 * autoclaving
 * pH
 * water bath / prevention of stuff growing
 * sterile technique
 * material
 * sterile filtration
 * defined media


 * Growing cultures
 * spreaders / glass beads
 * biosafety
 * rotators
 * aeration
 * pH
 * indicators
 * temperature
 * OD measurements
 * phases of growth


 * Receiving and sending strains and plasmids
 * recovering plasmids from spotted or dried DNA
 * growing stab cultures
 * preserving cultures in glycerol
 * preserving cultures with stabs
 * preserving cultures on plates
 * sending DNA
 * sending stabs
 * sending dry ice samples


 * Labels and lab notebooks
 * dated / initialed labels
 * lab notebooks


 * Agarose gels
 * percentage choice
 * buffer choice
 * microwaving the gel
 * adding dye
 * pouring the gels
 * bottle cleanup
 * running the gel
 * buffer choice
 * adding dye to the buffer
 * running time
 * other kinds of agarose
 * loading dye
 * dna markers
 * visualizing the bands
 * UV exposure issues


 * Cutting DNA from agarose gels
 * UV exposure issues
 * weighing gel samples
 * isolation of DNA from gel slices


 * PCR reactions
 * primer design
 * reaction components
 * setting up the reaction
 * choice of cycling conditions
 * hot lids