DIYbio:Notebook/Open Gel Box 2.0/Power Supply/Typical Setup


 * Typical Power Supply Setup in NW Lab
 * 1.2% gel is good for 100bp - 5kb (but by experience I would run 2% gel for 100-500bp)
 * The run time for 96v at 84mA for a 1000bp (see reference for typical prokaryote gene size I currently work with) band takes about 20 minutes to resolve, up to 40 minutes to get a good separation if you have multiple near-by bands.
 * 60V gets very good separation and clean looking 1kb bands, 120V runs fast but doesn't come out as well.



Select Typical Agarose Gel Concentrations&dagger; † Invitrogen http://tools.invitrogen.com/content.cfm?pageid=11229