User:Wilfred J. Poppinga/Notebook/Wilfreds Project/2009/08/08

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Comments

 * Frans his plates were barely grown on the bench, put them to 37 °C for 5 hours.

Results
Everything was written as I read it, no guarantees. After 5 h at 37 °C (and ON on the bench) Codes are written as they are read, not as they are interpreted. Plates with no growth were put back on the bench.

ALL PLATES WITH GROWTH, Nienke & Frans, were put @ 4 °C


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Discussion

 * Dyson et al.; Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression, BMC Biotechnology 2004, 4:32 doi:10.1186/1472-6750-4-32
 * Trx and MBP are best fusion proteins, recommended is to truncate proteins >50 kDa
 * They barely express soluble cell surface receptors


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