Registry/Measurement kit/Notebook/2007-7-28

Overnight cultures

 * Growth on:
 * E0240-1AK3
 * I2055-1AK3
 * I2057-1AK3
 * I2056-1AO3
 * Both promoter testers (3K3, ligated at NotI) versions E-X and E-S
 * None:
 * F2620-1AK3 and 3K3
 * Replate of F2620-1AK3

I2055-1AK3

 * Ran gel of last night's PCR, all colonies were wrong size.

Colony PCRs

 * Gel images:
 * Large gel:
 * I2057-ES (2,1); I2057-EX (6-1)
 * E0240-ES(4-1); I2057-ES(6-3)


 * Small gel:
 * E0240-EX (6-1); E0240-ES(6,5)
 * I2056-1AK3 (2,1); I2057-1AK3 (2,1); E0240-1AK3 (1); I2055-1AK3(3-1)


 * Made overnights of:
 * I2057-EX #6
 * I2057-ES #4
 * E0240-EX #1
 * E0240-ES #2
 * E0240-1AK3 #1 (not biobricked)

Transformations

 * Ligated ccdB into all promoter testers and transformed.