Template:SBB09 28190

David de Renzy

Tsh Autotransporter
Source: E. coli avain APEC genomic DNA You are going to construct a display part from the Tsh autotransporter. For background, you should read PMID: 15546669. See pubmed nucleotide sequence AF218073 for the DNA. Also check out the protein sequence at AAA24698: Region         855..1076 /region_name="PL1_Passenger_AT" /note="Pertactin-like passenger domains (virulence                    factors), C-terminal, subgroup 1, of autotransporter                     proteins of the type V secretion system of Gram-negative                     bacteria. This subgroup includes the passenger domains of                     Neisseria and Haemophilus IgA1 proteases...; cd01343" /db_xref="CDD:29328" Region         1113..1358 /region_name="Autotransporter" /note="Autotransporter beta-domain; cl02365" /db_xref="CDD:121263" You will construct your part to start with amino acid 855 and extend to the stop codon.

Ice Nucleation Protein
Source: pBca1256-Bca1346 You are going to make a tilda-style part (see notes below) derived from ice nucleation protein. INP is one of the most commonly-used display methods and you should read up on it starting with reference PMID: 14705004 and a pubmed search of "ice nucleation protein display coli".

You can download the template map for your part [[Media:pBca1256-Bca1346.seq | here]]. The 3' end of this part is already properly biobricked, so all you need is a new oligo for the 5' end. In your construction file, you should make use of this oligo: BBa_G00101 	Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc