User:Fermenter User/Notebook/MACG G1

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs  Pink: MeOH Induction hrs

MACG G1 (F1)

Day -4 (Jul 17 Fri):
             6/17/2009 (00:00)     Prepare and autoclave additives:

Day -1 (Jul 20 Mon):
             6/20/2009 (11:45)     Bring YP (1-L) to fermenter room           6/20/2009 (11:45)     Calibratiron of pH sensor Add 1 ml of Antifoam 6/20/2009 (12:40)    Autoclave start 6/20/2009 (14:10)    Start Fermenter #1 software 6/20/2009 (14:10)    Set rpm at 900 6/20/2009 (14:10)    Start temp control

             6/20/2009 (10:30)     Take a colony from plate in 600 ml of BMGY(4 500ml flasks). Allow it to gorw at 30°C.

Day 0 (Jun 21 Tue):
 MeOH Induction Day 1 ==

          6/20/2009 (09:30 -10:30) Feed additives using a pump Increase rpm to 1250 (Max) 6/20/2009 (10:40)    Calibrate dO2 sensor

             6/21/2009 (14:00)     Suspend down cells and resuspend in 15 ml of BMMY. Set up a 0.5 L culture in buffled flask.      6/21/2009 (14:30)     Fermentation 00:00 Inoculate into fermenter

Day 1 (Jun 22 Wed):
 MeOH Induction Day 2 ==      6/22/2009 (00:00)     Fermentation 00:00 Add 0.5% MeOH

Day 2 (Jun 23 Thu):
<font color=#FF00FF> MeOH Induction Day 3 == <Ganna/SungHye>     6/23/2009 (00:00)     Fermentation 00:00 Add 0.5% MeOH

Day 3 (Jun 24 Fri):
<font color=#FF00FF> MeOH Induction Day 4 == <Ganna/SungHye>     6/24/2009 (00:00)     Fermentation 00:00 Add 0.5% MeOH <Sung-Hye>          6/24/2009 (00:00)     Fermentation 00:00 Harvest


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