840:153g:Projects/project4/2009/02/17

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] MoldBusters' Journal
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Tuesday 2/17
Josh and Casy
 * The plasmid pSB1A2+R0080 samples were combined and digested with Pst1 and Spe1.
 * The samples were purified by running the DNA through a column.
 * Verification that the digestion was a success will be done on Thursday by running a gel.
 * The digested plasmid was stored in the -20°C freezer, and should be ready for the aprE ligation.

Oggie and Katy
 * Did research on possibility of genetic differences between globiformis & niger strains, and strain 168 (we used DNA from strain 168 as the template when developing our primers)
 * Did not find any results in regards to the two strains we are using
 * Found new DNA extraction protocol at Bio.net
 * Made TES buffer needed for new DNA extraction method


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