User:Dileep D. Monie/Notebook/2008-3-31


 * Prepared 500 ml of Supplemented M9 Media (this is the same as the Endy Lab version but does not require preparation of intermediate solutions)
 * Mix the following components, allow to dissolve completely, and sterilize with a 0.2 μm PES sterile filter
 * 497 ml ddH2O
 * 50 μl 1M CaCl2 (Teknova C0477; lot no. C047710L301Z)
 * 1 ml 1M MgSO4 (Sigma M3409; lot no. 116K6095)
 * 5.64 g M9 minimal salts, 5X (Sigma M6030; lot no. 127K1574)
 * 150 mg thiamine hydrochloride (Sigma T1270; lot no. 037K00071)
 * 1 g casamino acids (Acros 61204-1000; lot no. B0123863)
 * 2 ml glycerol (Invitrogen 15514-011; lot no. 1248326)
 * Protect Supplemented M9 Media from light and store at 4°C