Synthetic Biology Standardization Debate

This page collects thoughts and materials for a debate between the Arkin and Endy labs about standardization in Synthetic Biology.

Microscopy Standardization

 * 1) what reporters are more suitable for bulk measure? or single-cell? or flow cytometry?
 * 2) how reporter half-life, maturation time, brightness, predisposition to form aggregates affect measurements?
 * 3) what about multicolor imaging? how we solve the close emission spectra of the different red-emitting reporters?
 * 4) what about degradation tags, can they turn out to be useful for programmed and precise control of reporters half-life?

Transcriptional Standardization

 * Can this be standardized?

Translational Standardization

 * Can this be standardized?

Transcript Degradation Standardization

 * Can this be standardized?

Protein Degradation Standardization

 * Can this be standardized?

Plasmid Standardization

 * Which origins?
 * Do we have data on plasmid localization?

Genome Insertion Standardization

 * Do we have a standard locus for insertion?

Strain Standardization

 * Should we be working in the same genetic background?