User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/02/23

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Dissected and visualized heat shocked flp-out flies for confirmation
 * Fix 20' in formaldehyde solution.
 * Wash 3 x 5'
 * Incubate in 0.6% Triton w/ PBS
 * Wash 1x with PBS-T
 * Mount
 * Set up cross to re-introduce balancer for control cross
 * FM7/Y; Sc/Cyo, 42D/CyoAg X HsFLP, MH107; mRFP, myb+, 42D/CyoAG
 * Will collect non Sc, Bar-eyed males and females.


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