IGEM:IMPERIAL/2007/Calendar/2007-8-30

We are in Week 8  of the iGEM project

Project Calendar
name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7

Vesicles
Should the testing and characterisation of vesicles be pursued?

Reasons to abandon

 * Process of formation not very predictable - not even with same protocols
 * variation would affect characterisation
 * May not be able to carry out all tests for the applications
 * not enough time or resources (finances and equipment)
 * No method of counting vessicles - but can get less accurate results by population analysis
 * Found non-toxic pore protein - expensive
 * ID lagging in terms of testing of constructs - can use the man power in the lab
 * Would need a lot more man power to carry on research and do experiments - may still not finish on time!

Reasons to pursue

 * GFP may degrade after a few hours in-vitro - vessicles would increase life-span to ~ 3-4 days
 * life-span of at least a few days required for the CBD construct to fulfil current specification
 * but can modify the specification - threshold device rather than thermal history?
 * Haven't yet tried the cell extract in vessicles - may get better results!

Experiments

 * Phase 2 started - testing CBD constructs for temperature
 * Experiments going ok - but may run over in terms of the budget
 * ID construct - purification of LuxR may take time

Wiki

 * Tidy up 'public wiki' - seperate from 'working wiki'
 * Protocols hard to understand for outsiders - make more readable
 * Calender??
 * Anthony and Alex to re-design the wiki