User:Torsten Waldminghaus/Notebook/Chip on Chip with SeqA

{| width="800"
 * style="background-color: #cdde95;" align="center"|
 * style="background-color: #cdde95;" align="center"|




 * align="center" style="background-color: #e5edc8;" |

title=Search this Project


 * colspan="2" style="background-color: #F2F2F2;" align="right"|Customize your entry pages 
 * colspan="2"|
 * colspan="2"|
 * colspan="2"|

Project Description/Abstract
SeqA is a DNA binding protein that binds specifically to hemimethylated GATC sites in E. coli and other γ-proteobacteria. Analysis of the chromosome wide binding in vivo could provide further information on its function beyond regulation of replication initiation. For Chip on chip experiments (see ChIP-Chip E. coli) one only needs a good antibody and an array. In the first experiments the SeqA ab turned out to also detect RNA-polymerase or some associated protein. This means that good purification is needed before repeating the experiments. One try is an affinity substraction Affinity purification of antibodies.

Future Plans

 * Compare SeqA binding in fast and slow growing cells
 * Analyze SeqA binding in synchronized cells, if possible with the Baby cell column.