IGEM:MIT/2007/Ideas3

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 * Put Links and Papers you find for another category here
 * Put your initials next to a link if you're in the process of analyzing it

Sticky Proteins
Adhesive Properties of Pla

Metal Pumps and Metal Specific Promoters
Arsenic binding

Mussel Protein
Expressing proteins on E coli cell membranes

Mussel Protein

 * 1) Hwang04 pmid=15184131
 * 2) Hwang05 pmid=15932281
 * 3) Hwang07 pmid=17507090
 * 4) Hwang07a pmid=16979252
 * 5) Lee06 pmid=16920796
 * 6) Lin07 pmid=17360430
 * 7) Wang07 pmid=17475323
 * 8) Rice07 pmid=16600968
 * 9) Dane06 pmid=16448666
 * 10) Bessette04 pmid=15531628
 * 11) Etz01 pmid=11698382
 * 12) Hall07 pmid=17469847
 * 13) Samuelson02 pmid=12039531
 * 14) Wernerus04 pmid=15035661
 * 15) Gebhart96 pmid=9048419
 * 16) Kenan06 pmid=16873017
 * 17) Adey95 pmid=7737512
 * 18) Menendez05 pmid=15620878
 * 19) Deming99 pmid=10021411
 * 20) Burzio00 pmid=10998254
 * 21) Burzio97 pmid=9206011
 * 22) Marumo86 pmid=3089286
 * 23) Waite83 pmid=6298211
 * 24) Waite01 pmid=11258900
 * 25) Papov95 pmid=7650037

Mgfp-5 Amino Acid Sequence 1 sseeykggyy pgntyhyhsg gsyhgsgyhg gykgkyygka kkyyykykns gkykylkkar 61 kyhrkgykky ygggss

/lue

6.20.07

KNOWN PEPTIDES/PROTEINS AND CHARACTERISTICS (please update!)
+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
 * Name of peptide/protein
 * Paper/Reference
 * Length in amino acids
 * Obstacles for synthesis?
 * Relevant characteristics/Other pertinent information


 * Mgfp-5/Mefp-5
 * Hwang, et al
 * 75 aa
 * Obstacles AGK
 * "However, these attempts have failed to express functional and economical mussel adhesive proteins for several reasons, including a highly biased amino acid composition (5 amino acid types comprised �89% of the total amino acids), different codon usage preference between mussel and other expression systems (tRNA utilization problem), and small amount of adhesive produced" - Hwang
 * Presence of fp-5 has adverse effects on cell growth.
 * Positives AGK
 * Known sequence
 * Very adhesive
 * Has a 30% DOPA level, which no other foot protein comes close to
 * From M. galloprovincialis; Very adhesive; Low purification yield (but this last fact may not matter for us?)


 * fp-151
 * Hwang, et al 2007
 * fp-5 flanked by six decapeptide repeats from fp-1 AGK
 * | fp-1 sequence: Ala-Lys-Pro-Ser-Tyr-diHyp-Hyp-Thr-DOPA-Lys
 * diHyp = 3,4 Dihydroxyproline; Hyp= 4 hydroxyproline
 * Positives AGK
 * Purifies more easily than fp-5
 * Not harmful to cells


 * Mgfp-1
 * Hwang, et al
 * … aa


 * Mgfp-3
 * Hwang, et al
 * … aa


 * Mefp-1
 * Hwang, et al
 * 10 aa ?
 * Not very adhesive
 * Not very adhesive


 * Mefp-5


 * Mefp-1

FhuA

 * 1) Coulton83 pmid=6315686
 * 2) Etz01 pmid=11698382
 * 3) Ferguson91 pmid=9856937
 * 4) Moeck96 pmid=8939430
 * 5) Moeck97 pmid=9353297
 * 6) Locher98 pmid=9865695
 * 7) Coulton86 pmid=3079747
 * 8) Hashemzadeh98 pmid=9822833

New ideas for stickiness from Drew
NEW IDEAS: how do we use conditional stickiness???


 * baby machines??? but reason for it?  applications?
 * yeast surface attachement
 * coli background -- non-sticky / non-biofilm forming  (indole deficient)
 * attacking diseased cells: low oxygen-induced stickiness
 * quorum sensing stickiness
 * cancer surface markers


 * contact:	roy curtis (arizona state) -- salmonella as vaccine platform (have you ever wanted salmonella to stick to things)


 * stickiness as amplifier of rare phenotypes


 * fuel cell (zinc binding)
 * magnetotactic


 * geobacter (metal pilus -- iron oxide)
 * benign (neutral) biocoating for medical grade stuff
 * self-assembling bacteria (form 3d structure)
 * micron scale assembly (M13 phage factories...)
 * spatial polarity?  (bacteria -- old poles and new poles!)  markers?
 * yeast-- bud scar?
 * M13 easy


 * photogluing: 3D printing   (what to use for?)
 * checkpoint driven stickiness: (cell cycle synchronization)
 * sticks to arterial plaque and eats it :)
 * competant bacteria (snarfs up and integrates environmental DNA)