Kafatos:genomic DNA extraction from individual mosquitoes

Genomic DNA extraction from individual mosquitoes

 * 1) Put 1 mosquito into an eppendorf tube with 100ul 50% w/v Chelex 100 in water. 
 * 2) Squish fly with pestle and place at 100C for 10 minutes.
 * 3) Spin at top speed in a microfuge (>14,000xg) for 1 minute.
 * 4) Transfer 10ul supernatant to new tube and add 1ul Proteinase K (0.8 units).
 * 5) Incubate at 37C for 30min.
 * 6) Incubate at 100C for 5 min.
 * 7) Use 0.5ul as template in a 10ul PCR reaction.

Reagents (from Sigma): Chelex 100 (C7901-25G) sodium form chelating resin (iminodiacetic acid)

Proteinase K (P4850) from Tritirachium album, for molecular biology, >800 units/mL, buffered aqueous glycerol solution, DNAse-, Nickase- and RNAse- free.