User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/03

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Spermatogenesis

 * Dissect GFP-Myb for staining with the other Anti-BAM(1:5) Anti-PCNA(1:500).
 * Poor testis for some reason (old flies?)
 * 14 dissected total in two groups of 7
 * Dissect tdt-Myb for staining (30' at RT) with Oligreen(1:500) (treat with RNAse(1:10) during incubation)
 * Decent testis
 * 9 flies dissected
 * Fixed all testis in 4% Formaldehyde in PBST for 20'
 * Washed 3 x 5' in 1x PBST
 * Block O/N in 2% NGS


 * }