IGEM:Peking/2007/Switch-Notebook/2007-8-15

T1TE-pLX010 ^^ with ScaI and XhoI


l to r: T1TE-pLX010^^1-22, 100bp ladder, lambda marker

Right 552bp/Wrong 512bp

Select for sequencing: 4, 8, 10, 11, 12, 14

SS1-(GFP-pLX007) ^^ with SalI and XhoI


l to r: SS1-(GFP-pLX007)^^1-5, 100bp ladder, lambda marker

Right 896bp/Wrong 756bp

All are wrong..

Colony PCR test of SulA, Rec, lac, SS1-3, SD1-3-(GFP-pLX007)
Fragment     No. of colonies PCRed / selected

SulA                            24 /  4 lac                             24 / 11 Rec                             22 /  2 SS1                             24 /  0 SS2                             13 /  0 SS3                             18 /  9 SD1                             24 /  2 SD2                             24 /  4 SD3                             13 /  0