User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/23

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=WetLab: LovTap's promoters=

Ligation LovTap + promoters
LovTap (colony_1) was ligated to seven promoters with different strenght: J23_102, J23_105, J23_106, J23_110, J23_114, J23_116, J23_117. The promoters are in the the plasmid [Amp], these were digested with SpeI and PstI. and desphosphorylated. LovTap was digested with XbaI-PstI

Ligation Protocol

 * DNA(LovTap) ..............6μL
 * Plasmid (promoter) .....2μL
 * Buffer 10X T4 Ligase ...2μL
 * Enzyme T4 Ligase .....1μL
 * HPLC ........................9μL

TOTAL 20μL

The mixture were left ON @ 16ºC


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