Klapperich Lab:Notebook/Lab Meeting Notes/2009/01/08

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8 January 2009 Lab Meeting Agenda

 * Check with B&G about compressed air and vacuum?

† Announcements
 * Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. Stating 1/8/09. See Lab Calendar for dates and presentation schedule.

† Flu R01 † SEPSIS Project †RNA project † COBRA - problems: dead but green in the end - called tech support. - tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio. - omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute - try running the experiment first, then running the live/dead dye through second. Bold text - Petridish culture after running evaporation w/o dye in hydrophobic treated channel. - RIE (CHF3), Rain?, and Cytop coated and thermal bonding with PTFE filter tested. - Cytop turned out good bonding with PTFE filter.
 * Check on sample amount.
 * Plaque Assay.
 * Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09)--we're about to start the training--.
 * Sonali working on first draft of new version paper by 1/19.
 * Hussam working on Lambda phage data..
 * 1 ml methanol wash seems to be taking care of the issue.
 * More formal report to Jeff from Cathie.
 * Do cDNA prep. Spec, gels, send to Jeff B. Shipping?
 * Mark- Control circuit - This will be done by January.
 * Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)
 * Hydrophobic coating of microchannel
 * Test cheap wait for running
 * Slide for dispensing or integration scheme with SERS substrate

- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available. - hypothesis: drying controls clustering, reduction controls size? - need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant - set II done varying drying time and fast reduction time. SEM and SERS available. - difficulty in locating the bacteria - needs much higher particle/bacteria ratio - experiments outside the channel first?
 * Substrates (JZ)
 * DOE for the experiment.
 * Colloid experiment. inconclusive. Look at SEMs
 * Look at the unused substrates in SEM one week old or more.
 * Prospectus framework/discussion: target the end of this week 1/23/09.

† Valve Array/Valve building (FJ) † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR '''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD ''' † RCA † Senior project † F31: Cochlea † Silica Optimization (Lambda):
 * Teddy on track.
 * So so close!
 * Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week. Look at the bacteria as they flow.
 * CR mask was printed new design for trapper.
 * UV filter installed
 * Cathie needs a copy of MCK's, Journal of Applied Physics paper.
 * Experimental Data for paper.What do we need in terms of flow rate, seeding densities, etc.
 * Do 10 micron simulations.
 * Do 10 micron simulations.
 * IRB still going/ revisions by tomorrow to Ian. Target date 1/1/09
 * STAR-CD Renewal - January ($2K)
 * Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD
 * Try microscope for looking a fluor. Learn how to look at the data in Image J
 * Cathie submitted One pager Whitepaper. Waiting for response.
 * Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine
 * Set up adviser meeting Jan
 * Post Gent vs. Pre gent - 6 proteins. 4/6 no change.
 * Final two proteins: know by week of 1/19
 * Elution of C and D total DNA mass 2ng and 0.2 ng
 * Testing with water 12 channels- standard protocol- NA replaced with NF water
 * 10 channels - 5ch methanol-water / 5ch methanol-buffer-water
 * Next E. coli.


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