Paulsson:DNA modifying enzymes

Lurking somewhere deep within the icy confines of the -20 deg freezer at bench #2 are the following:

Restriction enzymes
AatII Acc65I AcuI Afe1 AflII AgeI AhdI AscI AsiSI AvrII BamHI BamHI-HF BclI BglII BlpI BsaBI BsiWI BspHI BsrGI BstAPI BstXI Bsu36I ClaI DpnI EagI-HF EcoNI EcoRI EcoRI-HF EcoRV HindIII HpaI KpnI-HF MfeI MscI NcoI NdeI NheI-HF NruI NsiI PacI PciI PmeI PshAI PstI SacI-HF SalI SalI-HF SbfI-HF ScaI SexAI SgfI SmaI SpeI SphI SspI StuI SwaI XbaI XmaI Xho1 ...more information at NEB for example: doing all your digests in Buffer #4, 5 minute digestions , activity in Taq buffer , activity in Vent buffer, and cleavage near the end of oligos

Restriction enzyme buffers
NEB: #1, #2, #3, #4, BamHI, EcoRI, 100xBSA (10mg/ml)

Polymerases
AccuPrime Pfx Supermix (Invitrogen) PCR Supermix (Invitrogen) coming in January '07... Phusion!!! not from Gilette, but from Mike Springer (include links to protein prep, protocol, plasmid map, etc...)

Other modifying enzymes
Alkaline Phosphatase, Shrimp (Roche) Mung Bean Nuclease (NEB) Plasmid-Safe DNAse DNAase from Ambion Benzonase Nuclease from Sigma T4 DNA Ligase (and 5x buffer w/ATP) - do what you can to keep them cold at all times (eg aliquot buffer upon first use) T4 Polynucleotide Kinase (NB: T4 PNK reaction buffer does not have ATP; add to 1mM OR use T4 Ligase rxn buf) Roche Rapid Ligation Kit

DNA markers
6x TriTrack Loading Dye (Fermentas) λ DNA-Mono Cut Mix 2-log ladder 50 bp ladder 1 Kb Plus DNA Ladder (Invitrogen) Supercoiled DNA Ladder