IGEM:Peking/2007/count:Miniprep

Mini Plasmid Purification
according to Transgen Kit Potocol.
 * 1) take 1-4mL over night cell culture ,13000rpm centrifuge for 1 min.discard the supernatant.
 * 2) add 250uL solution RB(contain RNaseA),mix violently.
 * 3) add 250uL solution LB，mix gentlely up and down for 4-6 times,此时溶液变粘，清亮,go to step 4 as soon as possible.
 * 4) add 350uL solution NB,mix gengtlely for 5-6 times.
 * 5) can see white precipitation.
 * 6) 13000 rpm centrifuge 5 min
 * 7) absorb supernatant into Spin column
 * 8) standing for 30 secends
 * 9) 13000 rpm centrifuge 1 min
 * 10) discard the flow-through,add 650 WB,
 * 11) 13000rpm for 1 min,discard the flow-through
 * 12) 13000 rpm for 2 min(in order to wipe out WB(contain ethanol),discard the spin column
 * 13) put the collection tube into a clean EP tube.add 30-50uL dH20(60C) in the center of the center of the collection tube.
 * 14) standing for 1 min.
 * 15) 13000rpm for 1 min.
 * 16) store the DNA solution at -20C