IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/20

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Entry title

 * Plasmid miniprep performed on the ligations which were done on 18/8. viz. the B set of composite parts.
 * Gel run was performed on these miniprepped DNA.
 * Not good results. Inserts were found to be bigger than the plasmid backbones
 * The following parts were sent for sequencing: (Primer: VF2 - 10pmole/microL; Template concentration - 100ng/microL)
 * 002 - A2;A5;A6
 * 003 - A6;A7
 * 004 - A3;A5
 * 005 - A4;A7
 * 006 - A1;A5
 * 001 - B1 to B6
 * 002 - B1 to B6
 * 003 - B1 to B4
 * 004 - B1 to B7
 * 005 - B1 to B7
 * 006 - B1 to B8


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