WangLab:Rules to Follow for Microscopy

Rules and Tips for the Microscope Operation

 * 1) Turn on the Microscope (following the orders):
 * 2) Arc Lamp Power.
 * 3) Shutter/Filter Wheels Controller.
 * 4) Halogen Shutter (Uniblitz, on the backside).
 * 5) Cascade Camera (on the backside).
 * 6) Zeiss Microscope (on the right side).
 * 7) Computer


 * 1) Shut off the Microscope (following the orders):
 * 2) lower the objective, rotate the objective inside
 * 3) take out the samples
 * 4) raise the objective again
 * 5) Clean the Oil Objectives (if any).
 * 6) Make sure the Objective is in Up position.
 * 7) turn off Computer.
 * 8) turn off Zeiss Microscope (on the right side).
 * 9) turn off Cascade Camera (on the backside).
 * 10) turn off Halogen Shutter (Uniblitz, on the backside).
 * 11) turn off Shutter/Filter Wheels Controller.
 * 12) turn off Arc Lamp Power

Others:

 * 1) If you used oil objectives, please make sure to clean it.
 * 2) 	cotton swabs + 70% Alcohol
 * 3) 	Lens Papers + Lens Cleaning Solution
 * 4) 	Dry Lens Papers
 * 5) 	Kohler Alignment
 * 6) 	Focus on the specimen using low magnification
 * 7) 	Close Field Diaphragm
 * 8) 	Adjust Condenser up and down until a sharp hexagon is observed
 * 9) 	Center the hexagon if needed with the two knobs
 * 10) 	open the diaphragm until the hexagon touches the field edge simultaneously.
 * 11) 	Components to adjust for DIC images.
 * 12) 	Polarizer (second ring filter on top)
 * 13) 	DIC Prism in the Condenser (e.g. 100X Objective, should choose DIC III on LCD display)
 * 14) 	Turn the prism under Objectives (Slide in to be functional).
 * 15) 	Polarizer in the Motorized Diachroic (Analyzer, DIC).
 * 16) 	To get a focus, use 10x objective first, focus, hit the “objectives” button to switch the objective to higher magnifications, fine adjust the focus to get a better view.
 * 17) 	If using low voltage/intensity for Halogen Lamp and seeing yellow color, put the First ring filter in position to balance the color.