IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation

 Specifications Design Modelling Implementation Testing/Validation  

Ligation strategy

 * Ligations for the prey
 * Ligations for the predator


 * More information on ligations can be found on the Ligation Page.

Quality control

 * Quality control for the prey part
 * Quality control for the predator part

Implementation

 * The oscillator will be tested in a chemostat. This equipment works to:
 * Keep the cells in the exponential growth phase
 * Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
 * Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat

WetLab work entries

 * The prey has been constructed and tested but is waiting parameter extraction.
 * The predator construct has not been ligated.
 * Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
 * We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.

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