Template:SBB-Protocols Zymo3

Zymo Gel Purification

 * All spins until the drying step are 15 second full speed spins.


 * 1) cut out bands minimizing extra gel matter.
 * 2) put in ependorf tube and add 600uL of Zymo ADB buffer (brown bottle).
 * 3) heat at 55, shake and/or vortex until the gel has dissolved.
 * 4) If the DNA is <300bp add 250uL of isopropanol
 * 5) transfer into the Zymo column inside a collection tube (small clear guys)
 * 6) spin through, discard waste.
 * 7) Add 200 uL of A4 Wash Bbuffer (which is basically 70% ethanol)
 * 8) spin through, discard waste.
 * 9) Add 200 uL of A4 Wash Buffer
 * 10) spin through, discard waste.
 * 11) spin for 90 seconds, full speed to dry.
 * 12) elute with water into a fresh Eppendorf tube