IGEM:PennState/Labbook/NoahJohnson/2007-7-6

July 6, 2007
 * Yesterdays motility plates found that motility was greatest at 26°C so today we are running 5 motility trials at 26°C with varying concentrations of eiken agar
 * Made motility plates
 * 0.25g tryptone
 * 0.2g NaCl
 * 0.15g sodium citrate
 * 25mL water
 * Eiken Agar:
 * 4 plates of 0.24%
 * 4 plates of 0.26%
 * 4 plates of 0.28%
 * 4 plates of 0.30%
 * 4 plates of 0.32%


 * Ran Gel on Pfx PCR products (Start 2:00)
 * Used Sybr-green
 * Well 1- DNA Ladder
 * Well 2- (-) Pfx
 * Well 3- 56°C Pfx
 * Well 4- 57.1°C Pfx
 * Well 5- 58.6°C Pfx
 * Well 6- 61.3°C Pfx
 * Well 7- 64.9°C Pfx
 * Well 8- 67.5°C Pfx
 * Well 9- 69.1°C Pfx
 * Well 10- 70°C Pfx