Jessica Karen Wong/Notebook/2007-8-7


 * Ran a gel of the overnight I2056 BB pcr's
 * Loaded: Lad EX SX
 * Both look right size
 * Heat Shocked I2055 S/X digest from PCR
 * Minipreped E0240-EX, E0240-ES, F2620 for seq
 * PCR cleaned I2056 pcr's and I2055-SX digest
 * Digested - E2056-EX both E/X and Not1, I2056-SX both S/X and Not1
 * Sent for seq each w/ VF and VR
 * F2620 to make sure it's in correct
 * E0240-EX colonies E2, E1
 * E0240-ES colonies M2, M1, E2
 * I2057-EX-J100 (1, 2)
 * I2057-ES-R40 (Bright, 1, 2)
 * I2057-EX-R40 (1,2)
 * I2057-EX-J116 (1,2)
 * I2055-SX-B31
 * Most I2055 plates had very few fluorescent protein
 * We realized that we didn't use restriction enzymes in ligation mix
 * Were going to do again w/ adding restriction enzymes to ligation mix, but didn't have enought DNA
 * Overnighted I2055-SNX and I2055-ENX
 * Overnighted 2 colonies of I2055-SX-B0030, 1 that fluoresced and 1 that didn't