User:Anugraha Raman/Notebook/iGEM 2010/2010/06/17

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Team Allergy
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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June 17 2010

 * Cloning:
 * We ran a gel on our pcr products from yesterday (Fra 1 antisense and sense extracted from fresh strawberries)
 * Based on the gel, it doesn't seem that the sequences were amplified (Wanted ~ .3 kb and saw .1 kb)
 * Reasons: primer dimers, cDNA extraction, allergen not expressed in particular strawberry
 * Digest w/ Xbai1 and Pst1/ purify to ligate into V0120 (biobrick plasmid)
 * Transform into E.Coli


 * Artificial miRNAs
 * It seems that we will probably create artificial miRNAs for silencing
 * We are waiting on the intron from the pHannibal vector


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