User:Tara K. Luckau/Notebook/Team ConGen/2010/10/28

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 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
 * style="background-color: #9DB68C" align="center"|  |Main project page
 * style="background-color: #9DB68C" align="center"|  |Main project page


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Cast Gel

 * medium caster: 20cm x 13cm x 0.5cm = 130mL TAE
 * 130mL TAE + 2.6g (2%) agarose in 250mL Erlenmyer flask
 * small stirbar, hotplate set at 200, stir set at 150, for about 15min, until clear
 * let cool (about 5 min)
 * add 13µL GelRed 10,000X, swirl
 * pour into caster, set 36-tooth comb
 * let set (about 10min)

Load Gel

 * 4µL Ladder
 * 4µL Sample w/ loading dye
 * [[Image:20101028 GelSchematic.jpg|800 px]]

Run Gel

 * 110V, 2 hrs

Image Gel

 * Nada, zip, zilch ... poopy
 * [[Image:20101028_Full.TIF|350 px]]
 * [[Image:20101028_Left.TIF|350 px]] [[Image:20101028_Right.TIF|350 px]]
 * WTF?


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