User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/13

 .todo { color: red } .done { color: green} {| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Putting cells to S0, splitting cells and continuing of protein isolation
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Summary

 * Protein pellets left @ 4 °C have been resuspended while in storage, only minor pellet remains
 * For final resuspending samples were incubated @ 50 °C and vortexed until resuspended
 * Cells of D9 P28 were put to S0
 * Cells were split to D9 P29 (800.000 cells per plate)