Eccles:cDNA Synthesis

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Part 1

 * Per Reaction:
 * 1&mu;L 250ng/&mu;L Random Primer
 * 1&mu;L 10mM dNTPs
 * ? &mu;L RNA (to give desired concentration)
 * MQ up to 14&mu;L


 * 1) 65&deg;C for 5 minutes (Called RT1 in Shujie's folder on red PCR machine).
 * 2) Put on ice.

Part 2

 * Per Reaction (6&mu;L):
 * 4&mu;L 5x RT buffer
 * 1&mu;L 0.1M DTT
 * 1&mu;L Superscript III enzyme


 * 1) Add to the above RNA solution.
 * 2) 25&deg;C for 5 minutes.
 * 3) 50&deg;C for 60 minutes.
 * 4) 70&deg;C for 15 minutes (Called RT2 in Shujie's folder on red PCR machine).