User:Tara K. Luckau/Notebook/Team ConGen/2010/11/04

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Set-Up

 * received sample of silicone mats (got 5! yay for free stuff!) from BioExpress, Catalog# T-3163-2
 * 10µL in each well of full-skirt PCR plate, PCR seal, silicone mat
 * Data: 20101104_TKL_CyclerTestBEMat.tad

Results

 * evaporated: A4, A5, A6, H6, A7, A8, H9
 * will probably use, anyway ...

PCR Scun2 F-R

 * optimization PCR with 3 variables: DNA concentration, Buffer, Temperature
 * determine lowest DNA concentration (1, 1/2, 1/10, 1/100) that works on PCR ... determine NanoDrop concentration ... use that value as goal concentration for all future PCR




 * DNA: from mass extraction (that Tina did), SCOC SYR9 X04
 * Ran out of Big MM for Buffer G, so H12 only has about 4µL
 * used new BioExpress silicone mats (catalog# T-3163-2)

NanoDrop SCOC SYR9 X04

 * this is the DNA sample used in the optimization gradient PCR, 4 November 2010


 * Only 1x and 1/2x gave positive concentration, but they are consistent with each other (2 x 1/2x ≈ 1x)


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