WRB100806-Construction of pSB1AK3-J23120

[OriT]-[pTet][Lock3d w/ 5' UTR ext][RFP][TT] digest pSB1AK3-J23039 (EcoR1/PstI, EcoRI buf, large 3152bp) digest pSB3C6-J23077 (XbaI/PstI, neb3, small 985bp) digest pSB1A2-J01003 (EcoRI/SpeI, EcoRI buf, small 394bp) ligate, product is pSB1AK3-J23120