IGEM:MIT/2007/Notebook/2007-8-22

Agenda
Uncompleted:
 * Clean out fridge: Threw out contaminated/overgrown plates, added fresh LB to LC's
 * Made glycerol stock of F2620+B0034+CPX+B0014 (for B-r cells cPCR6-1 and cPCR8-5, for BL21 cPCR6-1a, cPCR6-1b)
 * LC'ed yesterday's plating of transformations
 * Design Mer Primer
 * Transform PTO40
 * Transform reporter gene for mercury sensing system (Biobrick) - need to decide on which one first!
 * eventually must ligate it with an RBS? > actually, no, because we have RBS from start of merT protein.

Wrapped Up Making iGEM Antibiotic Stocks

 * Apparently iGEM needed to make own antibiotic stocks, so we did (SK)
 * All are stored in -20 iGEM freezer, in two boxes
 * Eppendorf box has A, Cm, K, T in 1 mL aliquots
 * Blue box has large aliquots of A, Cm, K, T in Falcon tubes and remaining 1 mL aliquots
 * Protocol from Francois: http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks
 * However, Cm protocol was made by SK -- not yet on OWW


 * NOTE CHANGE: We later found out that the stock should be made with 100% ethanol instead of 70%, but it shouldn't make a big difference(hopefully)