IGEM:Harvard/2006/vlau/Notebook/2006-7-25

Pre-Working Stocks & Working Stocks
1. Rxn Mixtures

2. Protocol - mixed 10 of each 50  stock oligo to make pre-working stocks - mixed listed pre-working stocks to make working stocks where final concentration of each oligo is 250 nM

Folding Rxns w/ Biotinylated Oligos
1. Reagents (for c4.0Fb, c4.0Gb, c4.0Hb, c4.0Ib) - 9 p7308 scaffold = (10 nM)/(44 nM) * 40 - 16 oligos = (100 nM)/(250 nM) * 40 - 4 10x folding buffer (500 mM HEPES pH 7.5, 500 mM NaCl, 100 mM ) - 11 d   - total volume: 40 2. Negative Controls - 1st (no oligos): 9 scaffold, 16  d, 4  buffer, 11  d   - 2nd (no scaffold): 9  d, 16  oligos, 4  buffer, 11  d