Van Oudenaarden Lab:D1007.1

Progress:

Probes designed: (03-01-2010 - Alexander van Oudenaarden)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

>D1007.1 (ceh-17 spliced coding region + 5'-UTR)

gcg

atgatgatggaatacggtggctatttttcaagtagcgccgtggctcaacaatcaggcgatgttccaacaactgcaccatc tgcagttacaaattcatttttctatacaccacagtcacataatatttatcaccaatatgcaactccgtatctacaaagtg gacgtgccttgacaactgctcacaatacttcaagctcttcagcgggcaattccacatcttcatcatcttcctcatcaaac taccgtaatacaacacacgattcactacaagctttttttaatactggtctacaatatcagttgtatcaaaaatcacaatt aattggaagtgatacgattcagaggacaagtagcaatgttttgaacggactgccaagaagttctcttgttggtgctctct gctcaaccggtggagctcctttgaaccctgccgagcgtcgaaaacaacgccgaatccgtaccacattcacatcaggacaa ctgaaggagttggaaagatcattctgtgaaactcattatccagatatttatacacgagaagaaattgcaatgagaattga tttaactgaagcccgagttcaagtgtggtttcagaatcggcgggcgaagtatcgaaaacaggagaagatcagaagagtta aggacgaggaagaggatccgttgaagaaggaacccgggcaaattagtttagaagagattattgatcaaatttga

Name of probe set: ceh-17

Probes designed on Mon, 01 Mar 10 08:14:13 -0700

32 probes designed for target of length 717 (target too short for 48 probes)

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

accgtattccatcatcatcg, ceh-17_1, 1, 2, 45

cggcgctacttgaaaaatag, ceh-17_2, 2, 24, 45

aacatcgcctgattgttgag, ceh-17_3, 3, 47, 45

taactgcagatggtgcagtt, ceh-17_4, 4, 72, 45

ggtgtatagaaaaatgaatt, ceh-17_5, 5, 94, 25

ggtgataaatattatgtgac, ceh-17_6, 6, 117, 30

tgtagatacggagttgcata, ceh-17_7, 7, 139, 40

agttgtcaaggcacgtccac, ceh-17_8, 8, 161, 55

gaagagcttgaagtattgtg, ceh-17_9, 9, 184, 40

atgaagatgtggaattgccc, ceh-17_10, 10, 207, 45

cggtagtttgatgaggaaga, ceh-17_11, 11, 229, 45

tagtgaatcgtgtgttgtat, ceh-17_12, 12, 251, 35

gaccagtattaaaaaaagct, ceh-17_13, 13, 273, 30

ttttgatacaactgatattg, ceh-17_14, 14, 295, 25

atcacttccaattaattgtg, ceh-17_15, 15, 317, 30

tgctacttgtcctctgaatc, ceh-17_16, 16, 339, 45

tcttggcagtccgttcaaaa, ceh-17_17, 17, 362, 45

agagagcaccaacaagagaa, ceh-17_18, 18, 384, 45

aaaggagctccaccggttga, ceh-17_19, 19, 406, 55

ttttcgacgctcggcagggt, ceh-17_20, 20, 428, 60

atgtggtacggattcggcgt, ceh-17_21, 21, 450, 55

tccttcagttgtcctgatgt, ceh-17_22, 22, 472, 45

ttcacagaatgatctttcca, ceh-17_23, 23, 494, 35

gtataaatatctggataatg, ceh-17_24, 24, 517, 25

tctcattgcaatttcttctc, ceh-17_25, 25, 539, 35

ctcgggcttcagttaaatca, ceh-17_26, 26, 561, 45

cgattctgaaaccacacttg, ceh-17_27, 27, 583, 45

ctgttttcgatacttcgccc, ceh-17_28, 28, 605, 50

ccttaactcttctgatcttc, ceh-17_29, 29, 627, 40

ttcaacggatcctcttcctc, ceh-17_30, 30, 649, 50

actaatttgcccgggttcct, ceh-17_31, 31, 671, 50

tttgatcaataatctcttct, ceh-17_32, 32, 693, 25