Small Scale Electrocompetent Prep-Generic

=Small Scale Electrocompetent Prep - Generic== before putting in 37oC incubator)
 * 1) Grow up strain to saturation
 * 2) Innoculate 2YT with 1:30 of o/n.
 * 3) Grow uninterrupted for 2.5 hours @ 37oC.
 * 4) Josh: use 4hrs @ 30C for ubercompetency
 * 5) Cool tube/flask; transfer 1.5 mL to chilled epi tube.
 * 6) Spin down for 20 sec at 14.1 rpm, discard supe, place immediately on ice.
 * 7) Resuspend with .5 mL 10% ice cold glycerol.
 * 8) Spin down for 30 sec (14.1 rpm), discard supe, place immediately on ice.
 * 9) Resuspend with .5 mL 10% ice cold glycerol.
 * 10) Spin down for 30 sec (14.1 rpm), discard supe, place immediately on ice.
 * 11) Resuspend in a small volume (90 ul) 10% ice cold glycerol.
 * 12) Add ~2uL DNA to cells, pipette & stir to mix.
 * 13) Immediately add to chilled electroporation cuvette.
 * 14) Immediately shock
 * 15) Immediately add 200 uL 2YT, pipette up and down to recover cells, move to tube and keep warm
 * 16) Put in shaking 37oC incubator for 1 hr
 * 17) Plate everything on appropriate antibiotic plate (allow to dry