Rao:Protocols

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General Techniques

 * Colony PCR Verification
 * Enzymatic Inverse PCR (EI-PCR)
 * Mega-Primer
 * Restriction Digest
 * DNA Ligation
 * Beta-galactosidase Assay
 * Tecan Safire2

E. coli/S. typhimurium specific

 * DNA Transformation
 * Transduction
 * One-step Gene Distruption
 * CRIM Plasmid Integration

B. subtilis specific

 * B. subtilis transformation

Media Preparation
If your interested in more recipes go here
 * LB
 * SOB/SOC
 * M9/M9c
 * Tryptone Broth (TB)
 * Penassay Broth
 * Motility Plates