IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/24

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Tuesday 24th August:
Plasmid- 10μL Pst1 - 2μL EcoR1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL
 * Transformations failed.
 * Ordered competent DH5-alpha e.coli from Bioline.
 * Set up an overnight restriction digest of pgaA promoter with EcoR1 and Pst1 restriction enzymes:


 * Ran an electrophoresis gel[] of plasmids pACYC duet-1 and pCDF. Results shown bellow:




 * The first three wells from the bottom were filled with the cut pACYC duet-1.
 * The three wells between the two ladders contain the cut pCDF.
 * pACYC duet-1 was extracted from the gel.[]


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