IGEM:IMPERIAL/2006/Protocols/20ul digestion

20 digestion

 * Place the following in an Eppendorf:
 * 5~8 DNA (inserts, if vector, max of 0.5)
 * 2 10x buffer
 * 2 Enzyme – add last (must be kept cold at all times) - (should be less than 10% of the total volume otherwise glycerol will inhibit the enzyme activity)
 * Make it up to 20 with MiliQ


 * 1hr at 37.