Matt Gethers/CRI, Thailand/Labwork/Dialysis

=Dialysis=


 * 1) Get dialysis tubing and cut more than enough to hold the volume you intend to dialyze (2 ml/cm of tubing). Be sure the tubing has a pore size appropriate for the molecular weight of your protein.
 * 2) Fill a beaker with RO water and wash the tubing. Be sure to separate the sides of the tube so the inside can be washed.
 * 3) Use a clamp to seal one end of the tubing. Be sure the tubing is flat and is in the middle of the clamp.
 * 4) Fill the tubing with your sample and clamp the open end.
 * 5) Fill a beaker with 1 L of dialysis buffer (see contents below) and place the clamped tubing in the beaker.
 * 6) Add 1 ml 1 M DTT to the liter of buffer.
 * 7) Place the beaker at 4oC on a stir plate over night.
 * 8) When dialysis is complete, unclamp one end, pour the sample into a tube and place on ice.
 * 9) To estimate the final concentration of the sample, divide the moles of salt both in the sample and the buffer by the total volume (1 L).

Be sure to add 1 ml 1 M DTT to 1 Liter when ready for use.

Run Notes
7.28.08

I made 2 liters of buffer as per the protocol above. I'm not prepared to dialyze samples yet, though, so I've placed the 2 liters in the four degree. No DTT added.