IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/13

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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July 13th, 2010

 * Worked on developing primer design and making sure there are no errors in the sequences
 * Prepared the project description for team review
 * Observed liquid cultures of E. coli with the transformed copper promoter attached to the CFP gene after adding copper solution to the mixture- observed fluorescence with UV lamp after spinning down the cells- treated with 1ml of copper sulfate solution
 * Observed X-gal plate with CFP round 2 PCR
 * Utilized the spectrofluorimeter to observe fluorescence of the E. coli with the copper promoter construct
 * Inoculated LB-Carb flasks with overnight RFP culture and placed under 3 incubation conditions (37 C with shaking, 37 C without shaking, and at room temperature)
 * Did readings of the fluorescence of our cultures of RFP under the 3 different conditions every hour
 * Met with high school students as part of an Education and Outreach program to discuss iGEM and future research opportunities
 * Prepared overnight cultures from the copper sulfate cultures (above) that fluoresced to retest copper promoter construct


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