IGEM:Peking/2007/Switch-Notebook/2007-7-9

Experimenter: NM & LCB

Precipitate with ethanol: pCC002^^ (C/N), SDD’-EGFP (B/C)

 * Time: 8:30am

NotI不能乙醇失活!! 必须用苯酚抽提失活, 呜呜… 做错了

重切pCC002^^ (C/N)!



l to r: pCC002 (wrong), pCC002 (right), none, SDD', SDD, Marker

KOD PCR of SDD-EGFP with temperature gradient

 * Primers: SDD-EGFP-BamHI-F, GFP-ClaI-R
 * Template: SDD-EGFP-pMD18T



l to r: SDD-EGFP(1-4), Marker

Digestion tests

 * Single digestion test of pLX007(1,2) with SalI/XhoI
 * Double digestion test of lacZa-pMD18-T with SalI/XhoI



l to r: lacZa(1-3), pLX007^ SalI1-2, pLX007^ XhoI1-2, Marker

PCR of CD1, RD1-3


l to r: CD1, RD1-3, Marker

PCR of CD1, RD1 with temperature gradient
Temperature:
 * 1: 50.3C
 * 2: 53.2C
 * 3: 58.1C
 * 4: 63.5C
 * 5: 68.1C



l to r: RD1(1-5), Marker



l to r CD1(1-5), Marker

Retract RD1(4), CD1(5)