IGEM:MIT/2007/Notebook/2007-7-14

Late Friday (7/13) Afternoon Update

 * BH/SK put dry pT040 (?) plates in warm room (for AK and AL)
 * Tom stopped by -- we have a BUNCH of chemically competent cells in the -80 degree freezer AND vectors pSB1AK3, pSB1AC3 and pSB1AT3 (already cut with E and P!) in the -20 degree iGEM freezer for our use

Weekend Update
So Bernice and I (SK) came in this afternoon. Here's a quick summary of what happened: Part         Kan+         Amp+ F2620     no growth      growth B0034     no growth      growth E1010     growth        no growth
 * Results of testing for antibiotic resistance of parts

This is what we wanted to happen, so yay!


 * Results of transformations (AK/AL)
 * Sample 1, 100 microliters and Sample 2, 100 microliters did not grow at all on A+/K+ plates

Sample         Volume              Number of Colonies 1              50 microliters      0 1              100 mics            4 or 5 1              200 mics            about 10 2              50 mics             2 2              100 mics            5 2              200 mics            10 to 12 These plates should be parafilmed and in the 4 degree fridge.

LC 1A = 36.9 ng/microliter LC 1B = 32.7 LC 2A = 27.0 LC 2B = 24.2 These are all in the -20 degree iGEM freezer in Eppendorf tubes.
 * Made permanent glycerol stock of transformed XL-1 cells after 3A transformation (in -80 degree freezer)
 * Made LC of AK/AL plates using Sample 1, 200 microliters (2 colonies) and Sample 2, 200 microliters (2 colonies) -- they're in the rotating shaker in the hot room (put in around half 4 on Saturday)
 * Miniprepped the diluted transformations of XL-1 cells after 3A transformation (so these have F2620 and B0034 in pSB3K3) -- from LC 1 and 2
 * The miniprepping we actually did twice because the nanodrop numbers were really, really bad the first time. From the second miniprep: