IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/16

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Monday 16th August:
Plasmid- 10μL Pst1 - 2μL NcoR1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL
 * Poured more antibiotic agar plates, (Km, Amp, Km + Amp)
 * Set up a restriction digest with pACYC, pCDF and pCOLA with EcoR1 and Pst1 enzymes:


 * Carried out gel electrophoresis [] of the digested plasmids.
 * Made overnight starter cultures of MG1655 WT.
 * Made new LB.


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