User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/16

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Dissect tdt-Myb flies
 * Fix 25' in 4% Formaldehyde in PBS
 * Wash 3 x 5' in PBS-T
 * Incubate 30' in PBS 0.6% Triton
 * Rinse 1x with PBS-T
 * Block O/N in PBS-T w/ 1% NGS.
 * Tomorrow
 * Stain with anti-alpha spectrin
 * Incubate 30' with RNAse A and Toto


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