User:Fermenter User/Notebook/DUON A1

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

DUON A1 (F3)

Day -1 (Feb 5 Thu):
Sung-Hye: CHBE Class Auditing 9:30 - 11:00

     2/5/2009 (12:10) Bring media to fermenter room           2/5/2009 (12:20) Assemble bioreactor 2/5/2009 (12:20) Add 2 ml of Antifoam. 2/5/2009 (13:30) Start Sterilization (Liq 45 min)

Day 0 (Feb 6 Fri):
2/6/2009 (7:00) Cooling of bioreactor (Temp setup) 2 L/min air, 800 rpm 2/6/2009 (8:45) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 2/6/2009 (8:45) Set dO2 2/6/2009 (8:45) Start OD module software 2/6/2009 (8:50) Start Fermentation Software for Fermenter #3 2/6/2009 (9:00) Completion of bioreactor set up: Ready for inoculation -               2/6/2009 (11:10) Add Amp (5 ml) 2/6/2009 (11:10) Inoculation: (50 ml) Fermentation 0:00      2/6/2009 (12:43) Sampling  Fermentation 1:33 AU (0.05) OD (0.27)      2/6/2009 (13:00) Sampling  Fermentation 2:50 AU (0.08) OD (0.50)          2/6/2009 (13:08) Fermentation 2:58  Induction 0:00 Add Inducer 20% Arabinose, 50 ml                                                                                       AU (0.10)      2/6/2009 (13:09) Sampling  Fermentation 2:59  Induction 0:01 AU (0.10) OD (0.55)      2/6/2009 (13:28) Sampling  Fermentation 3:18  Induction 0:20 AU (0.14) OD (0.75)      2/6/2009 (14:18) Sampling  Fermentation 4:08  Induction 1:10 AU (0.39) OD (2.2)      2/6/2009 (16:00) Sampling  Fermentation 5:50  Induction 2:48 AU (0.63) OD (3.9)      2/6/2009 (19:15) Sampling  Fermentation 9:05  Induction 6:03 AU (0.79) OD (4.4)

Day 1 (Feb 7 Sat):
<Sung-Hye>          2/7/2009 (11:00) Fermentation 24:50  Induction 21:48 Harvest


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