User:Daniel Catt/Notebook/Protein Biofilms Exploration/2011/06/14

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Protein Biofilms Exploration
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

06.14.2011

 * Prepared 3 potassium phosphate (KPi) buffers for use in bringing the copper/BSA solution to varying pH points.


 * The pH 5.8 buffer was, by dropwise addition of 1N HCl, adjusted down to pH 5. The solutions were not diluted from their 1M concentrations, but were instead used directly...


 * Soln #1: pH 5

- 1.5μM BSA

- 0.25mM cupric acetate monohydrate

- 5mL of 1M KPi at pH 5


 * Soln #2: pH 6

- 1.5μM BSA

- 0.25mM cupric acetate monohydrate

- 5mL of 1M KPi at pH 6


 * Soln #3: pH 7

- 1.5μM BSA

- 0.25mM cupric acetate monohydrate

- 5mL of 1M KPi at pH 7


 * The solutions were placed in glass test tubes and heated at 80°C in an oven overnight.


 * }