User:Alexander.wong/sandbox

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Our Projects


28 August 2007 - Infector Modelling

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 28/08/07 - Infector Detector Modelling!! 15/08/07 - We have vesicles!! <img src="http://www3.clustrmaps.com/counter/index2.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" width="160" /> </a>
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Cell Free Systems

Questions to ask the fluorometer Engineer on Friday, Aug 3
See if our plate reader has the ability to measure what you want to measure.
 * What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
 * Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
 * Temperature-regulation?
 * How do we subtract background values from data and convert from absorbance to OD600?

Talk about using the plate reader and creating protocols.
 * Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
 * Any manufacturer protocols for plate reader usage?
 * Well plate rows and columns? How is data arranged sequentially with the wells?
 * What kind of plates you need to order and use for our experiments?
 * Lamp energy and Noise signal ratios??

Talk about processing data
 * Export format?
 * How to export data from fluorometer?
 * Built-in data analysis tools?
 * Technical Support.

Experiment 1: Fluorescence vs extracellular [GFP] molecules
Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.

Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]
Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].

Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]
Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].