IGEM:Harvard/2006/PEG precipitation

PEG precipitation

 * Make a stock solution of 20% PEG (w/v), 2.5 M NaCl.
 * It may take a while to dissolve, but you can use a stir bar and low heat.
 * For 10 mL solution: 2.0 g PEG, 1.46 g NaCl
 * Add 0.25 volume of the PEG/NaCl solution to your folded nanostructure mix.
 * Incubate on ice for 15 minutes
 * Spin 16k rcf for 10 minutes
 * Pipette out supernatant into separate tube
 * Resuspend pellet in 1x folding buffer volume equal to the supernatant
 * Run equivalent volumes of pellet and sup on 2% agarose gel (+ 11 mM MgCl2)