User:Andy Maloney/Kinesin & Microtubule Page/K-PEM

Recipe

 * PEM
 * 1.0 mg/mL |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC kappa casein

Procedure
See the $\alpha$-PEM procedure for pictures.
 * 1) Weigh out 25 mg of kappa casein.
 * 2) Add 25 mL of PEM to the casein.
 * 3) Place in a beaker with a stir bar and stir for approximately 15-20 minutes on setting 7, or until all the casein is in solution.
 * 4) Aliquot into vials and store in the 4°C fridge.

Do not filter solution. This casein solution will last for 6 months in the fridge.