IGEM:MIT/2007/Notebook/2007-7-13

Checked Plates of Transformed 3A XL-1

 * LB+Kan Plate - all had significant growth
 * LB+Kan+Amp:
 * 1: very small colonies
 * 2: no growth
 * 3: some bits of dead cells on a streak, otherwise no growth
 * 4: many colonies
 * 5: no growth
 * 6: 9 colonies
 * 7: no growth
 * 8: no growth
 * The growth of colonies on #4 supports the theory that those cells received both pSB3k3 with F2620 and B0034 (Kan, medium copy) and religated pSB1A3 with just F2620 (Amp, high copy)

Diluted LC#1,2 of XL-1 3A in LB+Kan

 * Since those only grew on LB+Kan and not LB+Kan+Amp plate
 * for miniprep tomorrow
 * 37C at 3:30pm

Transformed Standard Assembly Ligation of E1010 into pSB3k3 from Yesterday

 * made 8 plates total, 2 samples
 * 37C @ 4:30pm

Received from Tom Knight digested pSB(1A3, 1AT3, 1AK3) vectors & Chemically Competent Cells

 * vectors digested with EcoRI and PstI, placed in -20C
 * cells placed in -80C, labeled iGEM2007

Meeting with Drew and TK Notes

 * check to see if Kan resistant already


 * ask for digested vector--tom knight is on it


 * terminators


 * make a color scheme as orgzn tool to display


 * Full product cycle
 * What to do at the end?
 * Hidden costs for disposal


 * Target market
 * rich
 * poor too
 * Find Mass PCR things


 * Different application
 * modification; disrupt bioaccumulation in food chain


 * make a salt or something, mercury crystal ->more valuable, recoverable, disposal


 * Diane Newman 3rd floor.
 * Magnetites, etc., go talk to her


 * Safety conversations
 * EHS website for Mercury


 * MerA leave it in?
 * Could be MerB/C


 * Price of Mercury/Cadmium


 * List of Target Metals
 * Then explain why this one, and not that ones
 * Bad/good/metal-specific opportunities
 * any number of good reasons/Markets/etc
 * Compare different ones


 * Does Mercury contamination exist in medium sized?


 * Other bacterial species
 * Pseudomonus--robust


 * So measurements/experiments
 * Plan of attack
 * Figure out how to quantitate, figure out parameters about stickiness


 * Measurement of ions


 * Figure out which version of mercury, +1? Most have been +2


 * Presentation Divisions
 * Put together part of the talk
 * Do earlier than later
 * Make our own slides