IGEM:NYMU/2007/Benchman

OD and GEL checks

Notes  A260/A280 should be around 1.70 A260/A230 should be less than 3.00 color key  problemistic (read) finished (yellow) await (blue)   digestion and buffer  SepI and PstI ( SP ) digestion using buffer 2, creates back vector </li> XbaI and PstI ( XP ) digestion using buffer 3, creates back insert </li> </ul> </li> Assembly process   STEP 1. prepare insert and vector by digestion </li>  STEP 2. check digestion by GEL separation  if band is clear at correct position (length), cut the gel and purify/extract it</li> else go back to STEP 1.</li> </ul> </li>  STEP 3. ligate insert with vector, transform it into competent cell, and extract plasmid from liquid culture</li>  STEP 4. check ligation by VF2, VR PCR with GEL separation  if band is clear at correct position (length), cut the gel and purify/extract it (ligation is done)</li> else go back to STEP 3.</li> </ul> </li> </ul> </li> </ul>