IGEM:UC Berkeley/2006/DNAGelPrep

Pouring Gels


 * 1) Fill 500ml  beaker to ~350ml with cold water
 * 2) Mix in 6g agarose
 * 3) Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear
 * 4) Add 10ml 50x TAE Buffer
 * 5) Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing
 * 6) Add 50ul 10,000x  SYBR Green (Bottle says ‘SYBR Safe DNA gel stain’, and liquid is very red)
 * 7) Pour into gel mold

-DLK_070306