User:Karmella Haynes/Notebook/Polycomb project/2009/11/19

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11/19/09

 * &#x2713; Transfections: test Gal4/mCh/VP64 ATF's (trial 2)

Transfections > HEK293 cells + Gal4/mCh/VP64 constructs > Use Fugene, 24-well format > Dilute each plasmid to 100 ng/ul in st dH2O

> Fugene + Opti-MEM --> R.T/ 5 min. > Add DNA --> R.T./ 20 min. > Add complexes to each well (1 ml med. each); 37&deg;C > Assay RFP/ Luc after 18-24 hours


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