User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/02/24

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Dissected 7 sets of 5 gfp-myb, rfp-h2av males
 * Will use for anti-PCNA, anti-mip40, anti-mip120
 * Fixed 30' at RT
 * Wash 2 x 5' in 0.1% PBS-T
 * Incubate 25' in 0.6% PBS-T
 * Place in 1% NGS O/N


 * }