Template:SBB11Projectstdt1110

Chia-Hung Tai

PY54_RepA part sbb1141 

PY54_RepA exists but needs PCR
This feature is a replication factor required for replication of a special origin of replication. For plasmids with that origin of replication, the plasmid will only replicate if the RepA protein is provided in trans. The protein is originally described in PMID: 15866931 and comes from a Yersinia bacteriaphage. Unfortunately, it is somewhat toxic to E. coli, and we want to know why and hopefully alleviate the problem. Though the sequence of this feature already exists, it does not exist as the rbs.cds part we need for our experiments. You can use the plasmid pBca9523-jtk2545 as the template in your construction file. In the end, it needs to be an rbs.cds! style part with vector pBca1766.

P_cspB part sbb1116