IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-19

Miniprep

 * For I7100-2;
 * Use Transgen Kit;
 * Final volume: 10mL

Digestion Test: I7100-2

 * I7100-2.
 * Digesting I7100 with EcoRI and EcoRI/XbaI.
 * Digestion system contains (totally 20uL):
 * 1) single digestion using EcoRI: 2uL 10*H, 0.5uL EcoRI, 6uL plasmid, 12uL ddH2O.
 * 2) double digestion using EcoRI/XbaI:2uL 10*M, 1uL EcoRI, 1uL XbaI, 6uL plasmid, 10uL ddH2O.

Electrophoresis result:

 * from Left to Right:
 * 1) I7100-2 @ EcoRI
 * 2) I7100-2 @ EcoRI/XbaI
 * 3) I7100-2 plasmid
 * 4) Marker
 * notes:**************

Digestion Test: E0040 & R0040

 * test if the new Dye helps to improve the resolution.
 * Digesting E0040 with EcoRI and EcoRI/PstI.
 * Digesting R0040 with EcoRI.
 * 1) single digestion using EcoRI: 2uL 10*H, 0.5uL EcoRI, 6uL plasmid, 11.5uL ddH2O.
 * 2) double digestion using EcoRI/XbaI:2uL 10*H, 0.5uL EcoRI, 0.5uL XbaI, 6uL plasmid, 11uL ddH2O.

Electrophoresis result:

 * from Left to Right:
 * 1) E0040 @ EcoRI
 * 2) E0040 @ EcoRI/PstI
 * 3) E0040-1 plasmid
 * 4) R0040 @ EcoRI
 * 5) R0040-1 plasmid
 * 6) I7100 @ EcoRI
 * 7) I7100 @ EcoRI/XbaI
 * 8) I7100-2 plasmid
 * 9) Marker

Electrophoresis - control

 * from Left to Right:
 * 1) E0040 @ EcoRI
 * 2) E0040 @ EcoRI/PstI
 * 3) R0040 @ EcoRI
 * 4) E0040-1 plasmid
 * 5) R0040-1 plasmid
 * 6) Marker

mimiprep

 * E0040-3, E0040-4, E0040-5
 * R0040-2, R0040-3, R0040-4