IGEM:MIT/2005/Friday 7/29 Meeting Agendas

Input

 * 1) chemically competent
 * 2) *cells with oligos fluresced, cells with fluorescein unknown
 * 3) competent vs. regular
 * 4) what next?
 * 5) *lets do this again
 * 6) *FACS this afternoon
 * 7) *Will and Max are going to meet with an advisor

Head Receiver

 * 1) scFvs are biobricks
 * 2) attach terminator to 3' end
 * 3) will attach promoter::rbs
 * 4) getting materials to test

ToxR

 * 1) MalE and PhoA are on their way
 * 2) Lets talk about what we're doing next week
 * 3) New option: have them built locally

FecA

 * 1) Whats done:
 * 2) *FecA wt, FecI, FecR
 * 3) To do:
 * 4) *quick change for FecA, FecR, PhoA
 * 5) FecA promoter error: need new promoters
 * 6) scFv linker: SG original linker not the best, according to some. Our linker should be fine. =o)
 * 7) Still obtaining Fur- strain -- hopefully soon

Signal Processing

 * 1) hoping our parts will come in
 * 2) ordered assembled parts

Actuator

 * 1) ready and waiting