IGEM:MIT/2005/Old Notes

../Sensor 2/ ../Annie's Notes/

What to be used

 * -Promoter X:
 * pBAD (may not be compatible w/ MC4100)
 * R0010 (inducible by IPTG)
 * R0011 (regulated by LacI)
 * R0040 (regulated by TetR)
 * -Terminator: B0015
 * -RBS: B0030

Things have to be done/decided

 * 1) FecA, FecI, and FecR relationship w/ respect to control of one promoter
 * 2) Alternative methods for gene fusion: random mutation by circular permutation and XL1-Red
 * Spec out FecA'-scFv
 * Design primers for internal fusion
 * Spec out FecA-scFv
 * LambdaRed to make our own FecA-
 * Obtain Fur- from Boston Medical Center -- POC Ray
 * Specifying composite parts
 * FecA, FecI, and FecR under same promoter may have issues
 * Make FecA-
 * Delete loops that's not necessary to make the FecA-scFv work