Smolke:Protocols

Note: If possible, please follow the standard protocol format.

Cloning

 * Primer Design
 * Plasmid prep with Epoch columns and homemade buffers
 * PCR amplifying inserts from plasmids
 * When to use phenol-chloroform vs. columns
 * Restriction digests
 * Gel extraction
 * Ligation
 * Transformation
 * Colony PCR
 * Screening by digestion
 * Sequencing with Elim
 * Making freezer stocks
 * Gene assembly

Yeast

 * Protocols
 * Yeast Media Recipes
 * Pouring yeast dropout plates
 * Yeast plasmid transformation
 * Yeast colony PCR
 * Anti-V5 Western
 * Library-scale yeast transformations
 * Yeast chromosomal modifications
 * Yeast plasmid prep
 * Yeast metabolite extraction
 * Observations and Discussion
 * CSY3

Tissue Culture room

 * Adherent cell care and feeding
 * Media stocks
 * Freezer stock management and database
 * General maintenance
 * General sterility

Quanta

 * Preventing Clogs
 * Clearing Clogs

Miscellaneous

 * Glass Beads
 * Poster Printing
 * My pipet-aid is broken!
 * Cleaning Bottles for the LC

Solutions

 * Pfu Buffer
 * Taq Buffer
 * 10x DO Media
 * dNTP Mixture
 * 5-Fluoroorotic acid
 * Antibiotic Stocks