IGEM:IMPERIAL/2007/Notebook/2007-10-17

Temperature step test for pTet-GFPmut3b

 * The aim of this test was to determine the response of the pTet-GFPmut3b construct to a step change in temperature.
 * For complete materials and methods see the Protocol
 * The construct was tested at two temperature steps:
 * From 4&deg;C (for 3 hours) to 20&deg;C (3 hours)
 * From 20&deg;C (for 3 hours) to 4&deg;C (3 hours)
 * The sampling time was every hour
 * The Results show that the step changes in temperature affect the fluorescence produced by the pTet-GFPmut3b construct