User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/01

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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ITS amplification of Yeast culture
Chelex Extraction from Yeast Culture

1)	Gently swirl growing culture. Pipette 50.0ul into tube. 2)	Spin down at 14,000 rpm. Remove supernatant. 3)	Add 200.0ul of Millipore water and pipette mix. 4)	Spin down at 14,000 rpm. Remove supernatant. 5)	Add 100.0ul Chelex. 6)	Boil at 100.0C for 10min. 27C for 1 min. 7)	Use 1.0ul for ITS amplification.

Load 2.0ul sample



Library confirmation by PCR
load 5.0 ul of sample



load 5.0 ul of sample