Kafatos:Protocols

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Lab specific protocols

 * Anopheles gambiae dsRNA gene KD and midgut dissection after malaria infection
 * Competent Cells
 * dsRNA Production by PCR
 * Extraction of Beauveria bassiana fungal spores
 * Instructions for the culture of Anopheles gambiae cell lines
 * totalRNA extraction from adult mosquitoes
 * totalRNA extraction from cells
 * genomic DNA extraction from individual mosquitoes
 * Minipreps
 * His-tagged Protein Purification (denaturing)
 * Making 1kb+ DNA Ladder Stock
 * Waste handling in the P3

Protocol template


 * Parasite Protocols of the Billker Lab.
 * Imperial College health and safety department
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Protocol collections on the web

 * a repository of OWW shared protocols
 * Nature Protocols
 * Protocol Online - Your lab's reference book
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