Sosnick:Protocols

Expression and Purification
Protein Expression

Calmodulin affinity purification

His-tag purification

Maltose Binding Protein tag purification

Purifying Ubiquitin

Purifying ctAcP

Chemical Synthesis and Modification
Free Sulfhydryl Determination

Solid Phase Peptide Synthesis

Cyanogen Bromide Cleavage of Ubiquitin

Analysis
Bis-tris SDS-PAGE

Sosnick:Rapid Coomassie Staining

Nucleic Acid
Agarose gel electrophoresis

Designing primers

PCR

PNK Treatment of DNA Ends

CIPing the vector

Ethanol precipitation of nucleic acids

DNA Ligation

Site-directed mutagenesis

Overlap extension PCR

E. coli
Chemically Competent cells (TSS)

Electrocompetent cells