User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Osmotic stress mapping

To do
The experiments will look at assays with varying amounts of betain in solution as well as the time course evolution of microtubule speeds. Once finished with betaine, I will want to move on to sarcosine. Sarcosine is only soluble in water to 1 M. So I guess I will try the following concentrations. If there are dramatic affects going on, then I will consider trying smaller concentrations. Of course, I will start with the 1000 mM solution.
 * 500 mM betaine
 * 2000 mM betaine
 * 4000 mM betaine
 * 1000 mM sarcosine
 * 500 mM sarcosine

D-proline is the final chemical I want to look at. It appears that this is soluble up to 8 M. This means I can do the following concentrations. If something interesting happens, then I will fill in the blanks when it comes to concentrations.
 * 8000 mM
 * 4000 mM
 * 500 mM

Really need to do
For all these solutions I need to measure the viscosity. I am trying to probe the osmotic stress affects on kinesin and microtubules and I need to know how these chemicals change the viscosity of my assays in the hopes to rule out viscous affects over osmotic affects.