User:Daniel Goodman/Notebook/Weiss/2010/01/14

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Prep for Plasmid Digestion Tomorrow
Will Run PhyB917, PhyB, and PIF3 on a Gel

PhyB917
Cut with BstBI - two bands, 3579 and 5314

incubate at 65 degrees with NEBuffer 4

Taking average, 400 ng/ul

Calculate digestion mix for 7.25 aliquots:

PIF3 (possibly)
Cut with AvrII - two bands, 2164 and 4791

incubate at 37 deg w/ NEBuffer 4

Nanodrop on PIF3 DNA sample: 357.4 ng/ul


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