SIL071106-Construction of pSB1A2-J23011

[TraMf] PCR JL5/JL6 on Olambda  (432bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521            (EcoRI/SpeI, Neb2, Large 2062) Product is pSB1A2-J23011 - JL5 Forward EcoRI oligo to bioBrick TraM: CCTAGGAATTCGCGGCCGCTTCTAGatggctaaggtgaacctgtatatcagc JL6 Reverse SpeI oligo to bioBrick TraM: gacttACTAGTgtcaaatttcgtttattcatc