IGEM:Peking/2007/Switch-Notebook/2007-7-14

Experimenter: CDZ, MHS, LC, CXY

Transformation of SDA-H, Y, cro, lacZa-pCC002

 * 8:15am, precipitate with ethanol: pCC002^^ (NotI/XhoI)
 * 2:00pm, ligation of SDA-H, Y, cro, lacZa-pCC002 with pCC002^^ (4uL/1uL)
 * 8:30pm, transformation

Sequencing result
CD1-2,4,5, SS1-2 are correct. Great! We have CD template.

RD1 is wrong. New primers already sent.

Miniprep and digestion test of pLX007-lacZa(1-53)

 * H buffer: 2uL
 * XhoI: 0.5uL
 * NdeI: 0.5uL
 * Plasmid: 10uL
 * ddH2O: 7uL
 * total: 20uL



l to r: pLX007-lacZa(1-23,25), marker

胶倒的不好. 没连上应该看到300bp的带，连上了应该有500bp. 都没有！质粒太少了？质粒不对？

Digestion test of pLX007(1)


l to r: pLX007(1)^^(NdeI/ScaI), marker

Not digested. The plasmid is wrong???