ESSCOSMOS/2009:Enzymes Soil & Fungal Enzymes

Overview
In this activity, we will study the organisms and environment along a gradient from the edge of a marsh to the top of the hill.

Objectives

 * Investigate the relationship between enzyme activity and CO2 release from substrate being decomposed by different species of mushrooms.
 * Create graphical summaries of the findings
 * Enter data into class database

Experimental protocol
We will be using a colorometric method developed by Dr. Steve Allison to measure the activity of enzymes in substrate that it colonized by mushroom mycelia. We will follow the methods outlined here: experimental protocol