IGEM:UC Berkeley/2006/Digestion

Digestion of a Mini-prep DNA (35 ul scale)

-  35 INCUBATE AT 37 degrees for 2 hours!
 * 1) 13 uL water
 * 2) 3.5 uL buff(3)
 * 3) 3.5 uL BSA
 * 4) 1 uL Enzyme 1
 * 5) 1 uL Enzyme 2
 * 6) 15 uL DNA

Digestion of a Mini-prep DNA (20 uL scale)

-  20
 * 1) 10 uL DNA
 * 2) 7 uL water
 * 3) 2 uL 10x buffer
 * 4) 0.5 uL Enzyme 1
 * 5) 0.5 uL Enzyme 2

Scaled up digestion of mini-prep

--   40
 * 1) 14uL H2O
 * 2) 4uL NEB Buffer 2
 * 3) 20uL DNA
 * 4) 1uL restriction enzyme
 * 5) 1uL restriction enzyme

Digestion of PCR Product

--  120 uL
 * 1) Elute 108uL of water
 * 2) 12 uL of 10X NEB Buffer 2
 * 3) 1 uL Enzyme 1
 * 4) 1 uL Enzyme 2