IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/09/14

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Miniprep

 * Resuspend with 100uL of H2O
 * Nanodrop:
 * 450 HRA: 594.45ng/uL
 * 450NCD: 1290.25ng/uL
 * 450 HRC: 682.85ng/uL
 * 450 HRB: 835.7ng/uL
 * 450 NCC: 1288.3ng/uL

Restriction Digest
Enzymes: Use EcoRI and SpeI (1uL each) DNA: 1uL of RFP chloraphenicol backbone, and 5uL miniprepped DNA Digest for 2 hours

Gel verification on RD
Gel orientation: Machine conditions: 0.5x TBE buffer, 100V, 45min Results:
 * Protocol: biobrick "digest" protocol

Transformations (HC)

 * Protocol: SOP; used competent cell #1
 * Add 5uL of ligation mixes
 * Plates put in 37C incubator at 11:46pm


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