User:Karmella Haynes/Notebook/Polycomb project/2010/05/11

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

05/11/10

 * &#x2713; ChIP: U2OS-plain sheared chromatin DNA quality control (finish)

ChIP: U2OS plain sheared chromatin DNA quality control > Started DNA qc over the weekend > Finished etOH precipitation yesterday; resuspend DNA pellet in 100 μL elution buffer (QIAGEN) > RNaseH-treat half of the sample (50 μL), 37°C/ 30 min. > Run 20 μL of each (+ 2 μL bromo-blue/ xylene cyanol 10x loading dye) on 1% gel

--> Majority of DNA is ~200 bp (mononucleosome size). Fine for 100 bp qPCR, but might consider reducing sonication a bit (target size usually around 1 kb).


 * }