Image:MaizeSCs.png

The resolution doubling of 3D-SIM is shown on an optical section of a corn (Zea mays) meiotic nucleus. The synaptonemal complexes are stained with antibodies against AFD1, a homolog of the meiosis-specific sister chromatid cohesion protein Rec8. At a separation of 190nm, the lateral elements of the synaptonemal complex are too closely spaced to be resolved by conventional microscopy (left); however, with 3D-SIM, the two parallel axes are clearly resolved (right).