Lauber: I won't forget


 * pvuII fast cutter, consider incubating for 30 minutes only
 * Ask Pam about what she does when checking for complete digestion. She transforms cells with her digested sample to check the background before T-tailing, but how? Does she ethanol precipitate, extract from gel and then transform?
 * Check EDTA, is it salt or what?

http://www.protocol-online.org/biology-forums/posts/4786.html