User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/05/24

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 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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PCR: Second plan
   -H2O -> 13.5μl  -Buffer ---> 6μl  -Mg(OAc) > 3μl  -dNTPs(0.4mM c/u) > 2μl  -oligo FWD (prefix, 5pmol/μL) ---> 2μl  -oligo RVS (suffix, 5pmol/μL) > 2μl  -DNA -> 1μl  -rtTh(high fidelity)-> 0.5μl    -positive: plasmid 18 -negative: blank <br/ >
 * Today, Miguel helped me to do the PCR (30 cycles) in order to amplify the luciferase (as band purification failed in two previous occasions).
 * The reaction was done as follows for a total of 30μl:
 * The controls were:


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