User:Karmella Haynes/Notebook/Polycomb project/2011/04/10

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04/10/11

 * &#x2713; RT-PCR: test primers on 129-4

RT-PCR > Test failed RT-PCR primers on KAH129-4 (36 rxns total) > Primers: 1-4. FGF10 36A-D (114, 117, 119, 80 bp) 5-8. INS 38A-D (106, 110, 107, 109 bp) 9-12. MYF5 39A-D (113, 113, 81, 81 bp) 13-16. HHEX 47A-D (86, 80, 87, 113 bp) 17-20. IL4 48A-D (97, 102, 106, 94 bp) 21-24. SLC6A4 49A-D (84, 86, 80, 83 bp) 25-28. NFE2 52A-D (90, 88, 118, 120 bp) ( Accidentally omitted NFE2 A, B ) 29-32. NEUROG1 53A-D (105, 117, 92, 98 bp) 33-36. SOX1 54A-D (84, 80, 80, 102 bp)

> Template:
 * 1) KAH129-4 +dox (4/08/11) 1:10

--> 1 template mix: aliquot 19.0 μL --> Add 1.0 μL primers

--> PCR (96-well)
 * 95°C/ 3 min.
 * [95°C/ 15 sec., 58°C/ 15 sec., 72°C/ 15 sec.] x32
 * 72°C/ 3 min.
 * 4°C/ ∞



--> Conclusions:
 * None worked. Discontinue testing these primers.


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