Calendar:ImperialWetLabNotebook2009iGEM/2009-8-11

Royah Vaezi:
 * James Field 03:59, 12 August 2009 (EDT):
 * Checked plates from Monday - pBAD failed to grow so re-plated (on both Kan & Amp plates in case registry was incorrect).
 * For the pBAD re-plating, I did two runs, one with 1.5ul DNA and the second with 1ul DNA.
 * The other two promoters extracted from registry on Monday had been transformed into E.coli without any problems.
 * Completed miniprep prep (5ml cultures & replica plates for all colonies to be used in Wednesday's miniprep).
 * Primers for PCR had arrived so made these up to stock solutions of 1ug/ul. Aliquots of working concentrations (100ng/ul) were obtained from these.
 * Primers are stored in the freezer.
 * PCR was carried out on the OtsB gene. Results were run on a gel to check the procedure had worked. Unfortunately no bands were obtained so this experiment will be repeated.