Jul 06,2008 Journal Club

7.06 Journal Club
In Attendance: Eric, Lu Yuanye, Yang Xiao, Li Yijie, Zhao Yue, Xing Jiqian, Jia Jia, Shao Wenjun, Tian He, Ma Qing, Wang Zengguang, Huang Qiushi Noted by Yang Xiao, Zhao Yue - Progress Report: The group encountered problems caused by recognition sequences on T carrier while digesting the T carrier ligation product. So they decided to use the plasmid for ligation instead. This week Group I will begin constructing different pADH promoters and linkers. Finally they got a right digestion result. Sequence analysis as well as synthesized lexO will be sent back this week.Group II will begin constructing the mutated gal4 system this week. Last week, Tian He went to NIBS to study the yeast cultivation and transformation. Unfortunately, our yeast had aged so badly that the concentration couldn't reach the level for transformation. Fresh yeasts are cultivated this week. Wiki has been constructed, and there're already some experiment record uploaded by Yue ZHAO and Xiao YANG. This week we'll continue uploading. And from today on, everyone please upload your daily experiment record in time ^_^ -- Journal Club Eric introduced the linkers he designed, and proposed the leucine zipper idea as a backup plan. Shao Wenjun introduced the NES progress during AID function. Yang Xiao introduced the phosphorylation progress during AID function. We noticed the relationship btw phosphorylation and NES, and maybe we could find a way to enhance the AID activity through such progress. So more references are required for Xiao YANG and Wenjun SHAO^_^ Tian He introduced the yeast cultivation and transformation protocol to us. -- New group: A MaQqing, Eric, LiYiJje B Tianhe, WangZengGuang, ShaoWenJun C LuYuanYe, HuangQiuShi D ZhaoYue, YangXiao Working time arrangement: please see Lu Yuanye's E-mail for details --- Notice: 1. Whoever needs new experiment materials, please write them on the blackboard in 319 2. Our icebox in 319 is nearly full, it needs cleaning up! -- JC this week Zhao Yue will organize the Journal Club the next week.
 * Group I ,by Ma Qing(hAID)
 * Group II, by Lu Yuanye(Gal4)
 * Group III, by Tian He(Yeast)
 * PART IV, by Xing Jiqian(wiki)
 * Team 1: Fusion Protein, Zengguang WANG, Qing MA, Eric
 * Team 2: Delete NES of hAID, Yuanye LV, Yue ZHAO, Wenjun SHAO
 * Team 3: Phosphorilation: Xiao YANG, Yijie LI
 * Team 4: Yeast Cultivation: He TIAN, Lei HUANG