25 February 2009

Date: 25 February 2009; Time:1400; Agenda: Exposure of biofilm to nanopolymers overnight for SEM.


 * 5 small bottles obtained from prof kunn's lab.
 * 1 is pure drug without polymer, 1 is loaded PLGA, 1 is loaded PCL, 1 is blank PLGA, 1 is blank PCL.
 * 2 rows of the CBD were allocated to each polymer/drug.

1st CBD:
 * Row B and C: Free drug
 * Row D and E: Loaded PLGA
 * Row F and G: Loaded PCL

2nd CBD:
 * Row B and C: Blank PLGA
 * Row D and E: Blank PCL


 * Each of the 6 rows above contained two-fold serial dilutions of the drug/polymer to vary the concentration of polymer.


 * After these 2 challenge plates are prepared, 2 lids of the CBD containing the biofilms (we prepared a total of 8 CBD for biofilm growth) were removed and replaced on the challenge plates. They were left to incubate in the 37deg incubator overnight.