User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/09

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM 2011
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Plasmid extraction pBBRmcS5. From DH5α.

 * First lane control
 * Plasmids 7 lanes.



Plasmid digestion

 * 7 digestions with HindIII
 * 2μL 10X Buffer 2 NE
 * 8μL H20
 * 10μL DNA Plasmid stock
 * 1μL Hind III.
 * Marks,
 * 1 tube without DNA
 * 1 tube without HindIII
 * 7 tubes with each plasmid stock.