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Vinidhra Mani

LacZ part jtk2791



This part exists but requires an EcoRI/BamHI transfer
LacZ is one of the oldest and most commonly used reporter genes in E. coli. This is b-galactosidase from the famous lac operon. LacZ is usually used at single copy since it's very potent. At high expression levels, it becomes toxic, and we're going to figure out why. This part already exists, but it is with the wrong vector. You'll need to do an Eco/Bam transfer to create the right plasmid. Your starting material is pBca9145-jtk2791, and we need to subclone it into pBca1766-Bca1089. P_phrB part sbb1135

P_oppBCF part sbb1111