User:Mbennie/Notebook/Papers and Books/Autotransporters General

Autotransporter-Review pmid=16369779
 * Focuses on AIDA-I protein which is an an autotransporter naturally expressed in e coli
 * Discusses applications using AIDA-1: expressing enzymes(whole cell biocatalysis), enzyme inhibitors(library screening), and epitotes(vaccine development)
 * Interesting note: passenger proteins can dimerize on the cell surface

Autodisplay-gramnegbac pmid=16787545
 * Autotransporter = N-terminal signal peptide + passenger domain + translocator domain
 * Study of autotransporters encourage because of their simplicity
 * Possible issues with autotransporters
 * Passenger must be in "translocation-competent state" in periplasm
 * Need specific signal sequences
 * Host can introduce genetic background
 * Other systems: Omp85, LamB, OmpA, PhoE, OmpC, TraT, FhuA, and intimin
 * Autotransporters (AT) belong to type V secretion system, specifically the type Va branch
 * Form beta-barrel to stabilize protein in the outer membrane
 * Passenger can undergo some level of folding in the periplasm
 * Most passenger domains are cleaved into media
 * "System is not amenable to the display of eukaryotic cDNA libraries."
 * 50,000 - 100,000 copies per cell

Early-Autotransporter-Review pmid=9778731
 * "No energy coupling or accessory factors are known to be required for the translocation process"
 * Some interesting autotransporters in e coli