User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/04

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Gel extraction of P. rapida DNA, continued from 03 Nov

 * make 10 ml 7.5M NH4OAc
 * 0.5 volume (8.5 ml) of 7.5M NH4OAc, mix,
 * then add 2.5 volumes (64 ml) of room temperature 95% ethanol
 * let stand 2 hours 11am-
 * split into two tubes (34 ml each) andspin 15 min at 15000 rcs
 * ultimately, dissolved in ~177 ul of water @ ~31 ng/ul, with some agarose remaining. About 5μg total yield
 * concentrated using a microcon kit
 * 19 min @ 0.5k g, checking every 3 min
 * invert tube, 3 min @ 1k g
 * 47 μl final volume

Blunt end repair of P.rapida DNA

 * however, skipped the denaturing step and continued to precipitation