User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/22

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 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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PCR success!!!
     1. Ladder. 2. Positive control.  3. Negative control.  4. PCR 1 using Prefix and the primer for the mutation (Reverse) with diluted DNA. 5. PCR 1 using Prefix and the primer for the mutation (Reverse). 6. PCR 2 using primer for the mutation (Forward) and Suffix with diluted DNA.  7. PCR 2 using primer for the mutation (Forward) and Suffix.  8. Purified plasmid J23106.    <br/ > <br/ > <br/ > <br/ > <br/ > 1. Ladder. 2. Positive control. <br/ > 3. Negative control. <br/ > 4. PCR 1.<br/ > 5. PCR 1.<br/ > 6. PCR 1.<br/ > 7. PCR 2.<br/ > 8. PCR 2.<br/ > <br/ >
 * I ran a gel in the morning to check that my PCR product was there. Fortunately it was, as is shown in the gel:
 * The lanes in the gel are as follows:<br/ >
 * As the product was there and the punctual mutation technique needs a lot of DNA, I decided to repeat the PCR realized yesterday. This time I only did the PCR1 and PCR2 (3 tubes each).
 * To check that everything went well, I ran another gel.
 * This time the lanes were:<br/ >


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