PrbbBB:Protocols

Biobrick assembly & related
We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.


 * prepare vector backbone for assembly (RFC 25 Fusion format)
 * Assembly protocol for BioBricks on 96 well plate (RFC 25 Fusion format)
 * Colony PCR screening of many assemblies in parallel (on 96 well plate)
 * Ligation-free Biobrick Construction by PCR/recombination (RFC 25 Fusion format)
 * DNA measurement on plates
 * Annealing of complementary long Oligos

Protein Expression

 * Small-scale expression in BL21
 * Large-scale expression in BL21
 * Solubilization of E. coli inclusion bodies
 * Coomassi protein stain (Ethanol-based)

Protein measurements

 * in-vitro FRET measurements