User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/08

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=Objectives=
 * Plasmid restriction, with SpeI.

Restriction

 * I need a 2nd restriction for |pUX19-XkR5 plasmid, because the restriction of yesterday shows an unexpected pattern of 3 bands.
 * I cut with SpeI because originally pUX19 has 2 sites, those sites flank insert of RepC-antisenseRNA, with that I expect 2 band:
 * Larger band with 3.9kb
 * Smaller band with 1.4KB(This is because insert for XKR5 plasmid has ~1.4Kb)

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 * Lane 1= Ruler
 * Lane 2= pUX19XKR5 cut.