User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/08/03

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Wowowoworking hard
  1. Green luciferase with ECO RI and PST I stored as luc wt R-> ECO y PST Mar and the date.  -H2O > 13μl -Buffer 3 --> 3μl -BSA ---> 1μl -DNA ---> 10μl -ECO I -> 1.5μl -PST I --> 1.5μl  2. Mutated-luciferase with ECO RI and PST I stored as mut-luz R-> ECO y PST Mar and the date.   -H2O > 13μl -Buffer 3 --> 3μl<br/ > -BSA > 1μl<br/ > -DNA > 10μl<br/ > -ECO RI > 1.5μl<br/ > -PST I ---> 1.5μl<br/ > <br/ > <br/ > <br/ > <br/ > 1. Ladder. <br/ >
 * Today, I repeated the restrictions done the other day with the purified PCR product of the mutated luciferase. <br/ >
 * The gel revealed that:<br/ >
 * The lanes were as follows:

2. Mutated luciferase 3/50. <br/ >

3. Mutated luciferase (purified product). <br/ >

4. Restriction of mutated luciferase. <br/ >

5. Restriction of luciferase. <br/ >

6. Restriction of pBSKII (+).<br/ > <br/ >


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