IGEM:IMPERIAL/2007/Projects/In-Veso/Specifications

=In-Veso Gene Expression: Specifications=

 Introduction Specifications Design Modelling Implementation Testing/Validation Notes References </ul> <br style="clear:both">

The specifications outline the characteristics of the chassis being sought, and the quantities that will be varied in experiments. The range for each input variable is also detailed. All lists are prioritised in order to help planning of experiments.

Input Variables
Temperature

Range from 4-37oC with priorities at:
 * 1) 25oC
 * 2) 4oC
 * 3) 6-20oC
 * 4) 30-37oC

Media

These are the different media the technique should be tested in, in order of priority:
 * 1) Water
 * 2) X-Gal
 * 3) E. coli Biofilm
 * 4) Agar Gel
 * 5) Polyacrylamide
 * 6) Paper

Note: take into account Osmolarity of the media.

Parts

List of parts from the registry we would like to test in-veso.

Piping

How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..?

This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc.

Dependent Quantities
Life span
 * Measured in hours

Rate of protein synthesis
 * The units will depend on the reporter being used, as well as the volumetric unit.

Rate of PoPs
 * Indirectly measured in GFP synthesis per CFU per second.

Membrane Traffic
 * List of particles that can cross the &alpha;-hemolysin pores

Resources
These are the resources that are available to us:
 * IC Specialists
 * Literature
 * Wetlab

Characterisation Chart
The following chart cross-references the input variables to the dependent quantities. Each cell will link to the protocols in the design section relevant to that relationship.

Things to look out for

 * Comparison with the efficiency of other chassis


 * Limitations of type of proteins made in the vesicles