Kubke Lab:Protocols/Hematoxylin Eosin

=Hematoxylin and Eosin staining=

WORK IN PROGRESS --- EXPECT FREQUENT CHANGES

=Modified Hematoxylin and Eosin staining procedure for chick embryo tissue= (Provided by Satya Amirapu,procedure not yet optimised)

1% ACID ALCOHOL
Mix together and store in a cool place
 * 3.5L Absolute alcohol	850ml
 * 1.5L distilled water	350ml
 * 25ml HCL	6.5ML

Gils 2 Haematoxylin

 * Distilled water                            730ml
 * Ethylene Glycol                            250ml
 * Pure Haematoxylin                          4gms
 * Sodium Iodate                              0.4gms
 * Aluminium sulphate                         70.4gms
 * Glacial acetic Acid                        20ml

1% Eosin
Dissolve Haematoxylin and Alum separately in distilled water and then mix.
 * EosinY-                                    20gms
 * Distilled water-                           2L
 * 1% Calcium Chloride                        20ml

1% Lithium Carbonate

 * Lithium carbonate                          1gm
 * Distilled water                            100ml

=References=

Disbrey & Rack (1970), Histological Laboratory Methods, E.&S.Livingstone, Edinburgh and London