Endy:Victor3 plate reader

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Introduction
The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement - We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome.
 * Time-resolved fluorometry
 * Fluorometry
 * Photometry
 * Luminometry
 * Fluorescence polarization
 * LANCE

Plate reader schedule
 How to sign up

Programming a basic protocol
Protocol editing

Creating and choosing labels
What is a label? Detection limits and linear range Setting an appropriate lamp energy Absorbance labels Changing filter slides

Running a protocol
Arranging samples

Counteracting evaporation in long time courses

Data Analysis
Basic data analysis Calculating fluorescent protein synthesis Measuring PoPS Background measurements Measurement variation Plate variation Anecdotal Converting from absorbance to OD600 Data analysis tools

When things go bad

 * Instrument serial number - 4206012
 * Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
 * Endy:Victor3 Service History


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