Lauffenburger:Brian Joughin

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Brian A. Joughin
Research Scientist, The David H. Koch Institute for Integrative Cancer Research at MIT

[mailto:bjoughin@mit.edu Email]

Current Research

 * Examination of the interpositional dependence of kinase specificity on substrate sequence.


 * Development of a bioinformatic method for discovering substrate motif specificity of uncharacterized kinases by integration of existing data.


 * Deriving biological insight from high-throughput quantitative phosphoproteomic mass spectrometry data.

Education

 * 2007, Ph.D., Department of Biology, MIT
 * Research Advisors: Profs. Bruce Tidor and Michael B. Yaffe
 * Doctoral thesis: "Novel Methods in Computational Analysis and Design of Protein-Protein Interactions: Applications to Phosphoregulated Interactions"
 * 2000, B.A., Department of Biophysics, Johns Hopkins University
 * Research Advisor: Prof. Eaton Lattman

Publications

 * 1)  Alexander J, Lim D, Joughin BA, Hegemann B, Hutchins JRA, Ehrenberger T, Ivins F, Sessa F, Hudecz O, Nigg EA, Fry AM, Musacchio A, Stukenberg PT, Mechtler K, Peters J-M, Smerdon S-J, Yaffe MB, Spatial Exclusivity Combined with Positive and Negative Selection of Phosphorylation Motifs Is the Basis for Context-Dependent Mitotic Signaling. Sci. Signal. 4, ra42 (2011). Abstract
 * 2)  Naegle KM, Gymrek M, Joughin BA, Wagner JP, Welsch RE, Yaffe MB, Lauffenburger DA, White FM. PTMScout: A web resource for analysis of high-throughput post-translational proteomic studies.  Mol Cell Proteomics. 2010 Jul 14. Epub ahead of print Abstract PTMScout
 * 3) Joughin BA, Cheung E, Karuturi RK, Saez-Rodriguez J, Lauffenburger DA, Liu ET.  Cellular Regulatory Networks in Systems Biomedicine: Concepts and Perspectives .  Liu ET and Lauffenburger DA, eds., Academic Press. pp. 57-108 (2009). Link
 * 4) Joughin BA, Naegle KM, Huang PH, Yaffe MB, Lauffenburger DA, White FM.  An integrated comparative phosphoproteomic and bioinformatic approach reveals a novel class of MPM-2 motifs upregulated in EGFRvIII-expressing glioblastoma cells.  Mol Biosyst. 2009 Jan;5(1):59-67. Epub 2008 Oct 30. Abstract
 * 5) Joughin BA, Green DF, Tidor B.  Action-at-a-distance interactions enhance protein binding affinity.  Protein Sci. 2005 May;14(5):1363-9. Epub 2005 Mar 31.  Abstract
 * 6) Joughin BA, Tidor B, Yaffe MB.  A computational method for the analysis and prediction of protein:phosphopeptide-binding sites.  Protein Sci. 2005 Jan;14(1):131-9. Epub 2004 Dec 2.  Abstract
 * 7) Powell DW, Rane MJ, Joughin BA, Kalmukova R, Hong JH, Tidor B, Dean WL, Pierce WM, Klein JB, Yaffe MB, McLeish KR.  Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding.  Mol Cell Biol. 2003 Aug;23(15):5376-87.  Abstract
 * 8) Pereira de Araujo AF, Pochapsky TC, Joughin B.  Thermodynamics of interactions between amino acid side chains: experimental differentiation of aromatic-aromatic, aromatic-aliphatic, and aliphatic-aliphatic side-chain interactions in water.  Biophys J. 1999 May;76(5):2319-28. Abstract


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