User:Fermenter User/Notebook/DOE Workshop 20110405

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WORK B1

1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop

Teaching:
 * Sung-Hye Grieco: Instructor, Suite Manager
 * Ann Y.K. Wong: Assistant Instructor, Ph.D. previously MacGillivray lab

When: April 5th - April 14th 2011

Where: LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332

Attending:
 * Ann Y.K. Wong: Assistant Instructor, Ph.D. previously MacGillivray lab
 * Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
 * Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
 * Swati N. Yewalkar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
 * Shiau-Yun (Mary) Tang (UBC Alumni)

Group A:
(After you log in with your personal password, click the "EDIT" button on the right side of GROUP A and list your name below)

Chris Tam Louise Creagh
 * Raymond Pan 13:16, 5 April 2011 (EDT):


 * Ann Y.K. Wong 13:16, 5 April 2011 (EDT):


 * Sung-Hye Grieco 13:11, 13 April 2011 (EDT):

Group B:
(After you log in with your personal password, click the "EDIT" button on the right side of GROUP B and list your name below)

MARY TANG Swati Yewalkar
 * Sung-Hye Grieco 13:14, 5 April 2011 (EDT):

Day 1 (April 5th, Tue) <-- WORKSHOP STARTS
10:00 - 11:00 Presentation (Rm 4.303) # Introduction about our Facility and Services # Introduction about bioreactors # How to assemble bioreactors Power Outage started around 8pm and lasted approximately 2.5 hrs. Bio-controllers and computer were connected to UPS (undisrupted power suppliers). Unfortunately, data has been lost due to power outage. Run #4 losted power during fermentation. End point looked ok. Run #5 was not affected by power. However, pH control was not done properly. Run #6 was not affected by power. End point looked ok. => Run #5 will be repeated at last as additional runs.

Day 2 (April 6th, Wed)
10:00 - 11:00 Presentation (Rm 4.303) # Fermentation Database and Websites # How to Install Bioreactors # How to Setup Bio-controllers

Another Power Outage started around 6:45pm and lasted approximately 1.5 hrs. Bio-controllers and computer were connected to UPS (undisrupted power suppliers). Unfortunately, data has been lost due to power outage. Run #7 losted power during fermentation. End point looked ok. Run #8 was not affected by power. End point looked ok. Run #9 was not affected by power. End point looked ok. => Run #7 will be repeated at last as additional runs.

Day 3 (April 7th, Thu)
10:00 - 11:00 Presentation (Rm 4.303) # DOE Step 1. Design Experiments

Due to unnotified power outage, two of emergency power lines were introduced into fermentation suite. One was connected to a computer and one was connected to incubator. Also, each fermenter is now connected to batteries to provide over 500 min capacity.

Day 4 (April 8th, Fri)
NO PRESENTATION

Day 5 (April 9th, Sat)
There is a scheduled power outage on Saturday morning between 8:00 AM and 10:00 AM => Power outage began at 8:01 and resumed at 10:00. No problem occured during this power outage

NO PRESENTATION

Day 6 (April 10th, Mon)
10:00 - 11:00 Presentation (Rm 4.303) # DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection

Day 7 (April 11th, Tue)
10:00 - 11:00 Presentation (Rm 4.303) # DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics

Day 8 (April 12th, Wed)
10:00 - 11:00 Presentation (Rm 4.303) # DOE Step 3. Analysis: Model Graph and Step 4. Optimization

Day 9 (April 13th, Thu)
10:00 - 11:00 Presentation (Rm 4.303) # Case Studies: Publication

Day 10 (April 14th, Fri)
9:30 - 12:00 DOE analysis with workshop data (Rm 4.307 Western Lantern)

Helful links

 * 1) http://meta.wikimedia.org/wiki/Table
 * 2) http://excel2wiki.net/  <--- Use this (Copy and Paste Excel to wiki converter)!


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