User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/09

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Objective

 * Clean and know purity of Daniel´s PCR

WetLab

 * Purification of PCR product with Roche´s kit of High Pure PCR product.
 * I joined tube A and B and were filled to 100μL


 * See purity of PCR cleaning with a gel.
 * Lane 1.5μL Ladder
 * Lane 2.3μL dye & 5μL DNA of cleaned PCR.
 * Lane 3.3μL dye & 5μL Control (Tube C from Daniel´s extraction).




 * Ligation of PCR purification:

I made two ligations by distinct ways.


 * DNA insert 5μL
 * DNA vector 1μL
 * Buffer10X  1μL
 * H2O        1μL
 * T4LigaseNEB 1μL

Second one
 * DNA insert 2μL
 * DNA vector 3μL
 * Buffer10X  1μL
 * H2O        3μL
 * T4LigaseNEB 1μL