User:Karmella Haynes/Notebook/miR Trigger

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Project Description/Abstract
Karmella & Francesca
 * Binding site optimization for silencing of Target: done &#x2713;
 * Luc-miR activation of sensor (stable lines in progress)
 * Transient transfection test (in progress)
 * Stable lines (in progress)

Experiment bookmarks
 * 2011 - miR Sensor devices 2.0: no insulators, polyA2, no PEST in AmCyan, MV8 vector
 * Status: still building
 * 06/02/11 - HPK/HPK miR Sensor activation; flow cytometry preliminary expts (-/+ dox in JDS33-47)
 * YFP (miR-luc expression) time course: 6/11/11 - 6/13/11; 6/22/11 - 6/24/11