Sortostat/Experiments/Brian growth tests

03/24/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

 * Strain: MC4100
 * Device: I6032
 * Media: Supplemented M9 + Amp
 * Temp: 37C


 * Doubling Time: Want:1hr           Get:55.45min


 * There are only YFPs in this run.

Chemostat Settings
"Dilution Rate": 1/hr
 * ''There are no sorts in this run.
 * Flow Rate: 11.09nL/hr
 * Actual: 12.12nL/hr
 * Dilution Rate: 0.69/hr
 * Actual: 0.75/hr
 * "Clean Chemostat Max Period": 1.44928hr
 * "Actual": 1.32hr


 * Minimum Pumping Time: 70s
 * "Actual": 105s; this gives us 2 screens/4.95min
 * Dead Time per Screen: 20s
 * Dead Time per Sort: 47s
 * Pump Cycle Period: 400ms
 * Sorting Perdiod: 4.95

Note that the pump worked abnormally well under these pressure conditions.
 * Feeder Fill: 10s
 * Wash: 10s
 * Clean: 15s
 * Pressurize: 5s
 * Fluid Pressure: 5.82 psi
 * Push-UP: 14.50 psi
 * Push-DOWN: 20.44 psi

Results


03/17/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

 * Strain: MC4100
 * Device: I6032
 * Media: Supplemented M9 + Amp
 * Temp: 37C


 * Doubling Time: Want:1hr           Get:55.45min

Chemostat Settings
"Dilution Rate": 1/hr
 * ''There are no sorts in this run.
 * Flow Rate: 11.09nL/hr
 * Actual: 12.12nL/hr
 * Dilution Rate: 0.69/hr
 * Actual: 0.75/hr
 * "Clean Chemostat Max Period": 1.44928hr
 * "Actual": 1.32hr


 * Minimum Pumping Time: 30s
 * Dead Time per Screen: 20s
 * Dead Time per Sort: 47s
 * Pump Cycle Period: 400ms
 * Sorting Perdiod: 4.95


 * Feeder Fill: 10s
 * Wash: 10s
 * Clean: 15s
 * Pressurize: 5s
 * Fluid Pressure: 5.30 psi
 * Push-UP: 14.07 psi
 * Push-DOWN: 20.44 psi

Results
Control lines emptied and entire run failed.

03/16/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

 * Strain: MC4100
 * Device: I6032
 * Media: Supplemented M9 + Amp
 * Temp: 37C


 * Doubling Time: Want:1hr           Get:55.45min

Chemostat Settings
"Dilution Rate": 1/hr
 * ''There are no sorts in this run.
 * Flow Rate: 11.09nL/hr
 * Actual: 12.12nL/hr
 * Dilution Rate: 0.69/hr
 * Actual: 0.75/hr
 * "Clean Chemostat Max Period": 1.44928hr
 * "Actual": 1.32hr


 * Minimum Pumping Time: 30s
 * Dead Time per Screen: 20s
 * Dead Time per Sort: 47s
 * Pump Cycle Period: 400ms
 * Sorting Perdiod: 4.95


 * Feeder Fill: 10s
 * Wash: 10s
 * Clean: 15s
 * Pressurize: 5s
 * Fluid Pressure: 5.07 psi
 * Push-UP: 14.07 psi
 * Push-DOWN: 20.02 psi

Results
Run Failed. Cell counts were impossibly large. Images show that there were no cells present soon after run started. This is probably due to cell growth that was so slow it could not keep up with the dilution rate. A lack of oxygen could be playing a role here.

03/12/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

 * Strain: MC4100
 * Device: I6032
 * Media: Supplemented M9 + Amp
 * Temp: 37C

Chemostat Settings

 * There are no sorts in this run.
 * Dilution Rate: .69/hr
 * Clean Chemostat Period: 1.45hr
 * Minimum Pumping Time: 30s
 * Dead Time per Screen: 20s
 * Dead Time per Sort: 47s
 * Pump Cycle Period: 400ms


 * Actual Cleaning Period: 1.32hr
 * Sorting Perdiod: 4.95


 * Feeder Fill: 10s
 * Wash: 10s
 * Clean: 15s
 * Pressurize: 5s
 * Fluid Pressure: 4.47 psi
 * Push-UP: 13.91 psi
 * Push-DOWN: 19.7 psi

Results
Catastrophic Failure! Airline blew off actuator and all tank pressure was lost.



01/24/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

 * Strain: MC4100
 * Device: I6032
 * Media: Supplemented M9 + Amp
 * Temp: 37C

Chemostat Settings

 * There are no sorts in this run.
 * Dilution Rate: Varies, (see graph)
 * Clean Chemostat Period: 1/D.R.
 * Minimum Pumping Time: Changed from 60s to 50s when the dilution rate changed from 0.4/hr to 0.5/hr
 * Dead Time per Screen: 10s
 * Dead Time per Sort: 75s
 * Pump Cycle Period: 400ms


 * Actual Cleaning Period: ?
 * Sorting Perdiod: ?


 * Feeder Fill: 10s
 * Wash: 10s
 * Clean: 15s
 * Pressurize: 5s
 * Fluid Pressure: 5.19 psi
 * Push-UP: 15.47 psi
 * Push-DOWN: 19.39 psi

Results
Equipment failure