Kubke Lab:/Notebook/Cranial nerve development/2010/12/06

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Cranial Nerve Development
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=General Entries= =Personal Entries=

Staining Sections
I performed an H&E stain on the sections cut on Friday, these were from the embryo RC002 kept in PFA without cryoprotection. They did not fall of the slides which would suggest that using subbed slides did help with adhesion. The Haematoxylin staining was fine however the counterstain with Eosin needed to be stronger and so next time I will keep the sections in the Eosin for longer and perhaps not leave them/wash them in the ethanol for as long (maybe 1sec).

I will now stain some of the sections Malisha has cut to see if the cryoprotection made a difference.--Reuben Cutfield 18:48, 5 December 2010 (EST)

I did not stain Malisha's sections and in lieu I will stage and place in sucrose another embryo (RC003) to which I will perform thick cuts (16-20um) and mount using the razor blade onto polylysine-subbed slides. --Reuben Cutfield 19:46, 6 December 2010 (EST). .

Malisha
10.20 am: MH002 was moved from the freezer to the cryostat and cut the details can be viewed here


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