Template:SBB10 ConstructionFiles ZhenH sbb15

sbb15: phiC31 attB Source: Synthetic Target Sequence: tgacggtctcgaagccgcggtgcgggtgccagggcgtgcccttgggctccccgggcgcgtactccacctcacccatctggtcca Vector: pBjk2741 Short descriptions: phiC31 attB Genbank reference: AB306970 (759…842) Family: Phage att site

good 20 bp gtgccagggcgtgcccttgg

Wobble ZZH003/ZZH004           (115bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144        (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb15     {phiC31 attB}

ZZH003  Forward construction of phiC31 attB basic part ccataGAATTCatgAGATCTtgacggtctcgaagccgcggtgcgggtgccagggcgtgcccttgg ZZH004  Reverse construction of phiC31 attB basic part ctgatGGATCCtggaccagatgggtgaggtggagtacgcgcccggggagcccaagggcacgccctggcac



JCA Notes

 * Correct