User:Fermenter User/Notebook/BROM C6

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM C6

Day 0 (Mar 29 Mon):
            3/29/2010 (10:00) Bring media to fermenter room            3/29/2010 (11:00) Assemble bioreactor 3/29/2010 (11:00) Add 5 ml of Antifoam/fermenter 3/29/2010 (12:10) Start Sterilization (Liq 45 min) 3/29/2010 (13:55) Cooling of bioreactor (Temp setup) 1 L/min air, 300 rpm 3/29/2010 (13:58) Start Fermentation Software for Fermenter #6 3/29/2010 (13:59) Start Fermentation Software for Fermenter #7 3/29/2010 (14:30) Connect Acid (250 ml) and Base (400 ml) Start pH control 3/29/2010 (14:30) Calibrate dO2 probe with 300 rpm, Air purge for 30 min 3/29/2010 (14:30) Set dO2

            3/29/2010 (15:00) Inoculation: inoculation volume (300 ml) Fermentation 0:00 F6: Cell weight (?? mg), Activity (26%, x100), Activity (11%, x1000) F7: Cell weight (?? mg), Activity (37%, x100), Activity (23%, x1000)

Day 1 (Mar 30 Tue):
            3/30/2010 (10:00) Sampling  Fermentation 16:00 F6: Cell weight (?? mg), Activity (93%, x100), Activity (75%, x1000) F7: Cell weight (?? mg), Activity (86%, x100), Activity (45%, x1000)

            3/30/2010 (13:30) Sampling  Fermentation 22:30 F6: Cell weight (?? mg), Activity (93%, x100), Activity (75%, x1000) F7: Cell weight (?? mg), Activity (96%, x100), Activity (88%, x1000)

   3/30/2010 (14:00) Fermentation 23:00 Harvest Fermentation F6: Acid (200 ml), Base (200 ml) F7: Acid (250 ml), Base (280 ml)


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