User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/18

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Heather incubated with primary antibodies O/N. Using anti-bam and anti-vismay on the two samples.
 * Wash 3 x 10 minutes in 1x PBST
 * Incubate with secondary antibody in 2% NGS (1:500, 488 - Bam; anti-mouse, vismay; anti-guinea pig)
 * 2hr at RT or O/N at 4C
 * Wash 3 x 10 minutes in 1x PBST
 * Stain with ToPro3 1:1333 in 2% NGS
 * 25 mins at RT
 * Wash 4 x 5 minutes in PBST
 * Mount in Vectashield


 * }