Quint Lab:yeast media

{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"
 * -valign="top"
 * width=750px style="padding: 5px; background-color: #ffffff; border: 2px solid #F8B603;" |

yeast media
10x DO (Clontech) → 121°C 20 min → 100 ml /1 l YSD
 * 1x:	0.6 g DO Supp + 26.7 g Min SD Base/ 1 l H2O
 * 10x:	1.2 g DO Supp / 200 ml H2O

→ autoclave (or steril filtration)

50% PEG (for 400 ml): (Sigma #P-3640) → autoclave
 * PEG 4000 - 200 g

10x TE (for 1 l): → add 880 ml ddH2O → autoclave
 * 1M Tris-HCl pH 7.5 - 100 ml
 * 500mM EDTA pH 7.5 - 20 ml

10x Lithium Acetate (for 400 ml): → pH 7.5 with diluted acetic acid → autoclave
 * Lithium Acetate (Sigma #L-6883) - 40.8 g

→ every time fresh!!!