Size selective DNA precipitation protocol

Solutions/reagents:  DNA sample  (DNA to be separated (e.g. PCR reaction mixture))  PEG/MgCl2 '' (30% (w/v) PEG 8000/30 mM MgCl2 (concentration of PEG 8000 can be varied to shift the size of the percipitated DNA. The concentration used here will remove DNA fragments with less than 300bp))  TE buffer  (10 mM TRIS-HCl, 1 mM EDTA, pH 8.0) ''buffer of choice Equipment: Centrifuge</li>Eppendorf tubes</li></ul> Steps: <ol> Measure out <font color=#357EC7>50 µl  of <a href="#DNA sample" ><font color=#357EC7>DNA sample </a> into Eppendorf tube (1). Measure out <font color=#357EC7>150 µl  of <a href="#TE buffer" ><font color=#357EC7>TE buffer </a> into DNA sample. Gently tap the mixture for a few secs. </li> Measure out <font color=#357EC7>10 µl  of <a href="#PEG/MgCl2" ><font color=#357EC7>PEG/MgCl2 </a> into Eppendorf tube (1). </li> Vortex the mixture for a few secs. </li> Centrifuge at a speed of <font color=#357EC7>10000 Xg for <font color=#357EC7>15 mins  at <font color=#357EC7>room temperature , gently aspirate out the supernatant and discard it. </li> <font color = "#800517">Carefully remove supernatant not to disturb the pellet, which will be invisible. </li>  Add <font color=#357EC7>buffer of choice to pellet. <font color = "#800517">Add appropriate volume of buffer. Dissolve the pellet in the solution. </li> </ol> TOTAL TIME REQUIRED FOR THE COMPLETION OF THE PROTOCOL :<font color=#357EC7>~ 15 mins