NanoBio: BG-11 media

=BG-11 liquid media=
 * Cyanobacteria, including Synechocystis PCC 6803, are typically grown in BG-11 media.
 * Obtain 50x BG-11 media from Sigma-Aldrich (catalog # C3061-500ML).
 * In the biosafety cabinet, mix 20 mL 50x BG-11 media and 980 mL Millipore water.
 * Autoclave for 15 minutes at 121C in 500 mL bottles to sterilize.


 * Contents of 1x liquid media:
 * NaNO3       1.5 g/L
 * K2HPO4      0.04 g/L
 * MgSO4.7H2O  0.075 g/L
 * CaCl2.2H2O  0.036 g/L
 * Citric acid 6.0 mg/L
 * EDTA        1.0 mg/L
 * Na2CO3      0.02 g/L
 * Ferric ammonium citrate 6.0 mg/L
 * H3BO3       2.86 mg/L
 * MnCl2.4H2O  1.81 mg/L
 * ZnSO4.7H2O  0.222 mg/L
 * Na2MoO4.2H2O 0.39 mg/L
 * CuSO4.5H2O  0.079 mg/L
 * Co(NO3)2.6H2O 0.0494 mg/L
 * Distilled water 1.0 L
 * sources: Sigma-Aldrich & ATCC (media 616)

=BG-11 plates=


 * For 1 L, or ~2 sleeves of plates, place in a 2L flask:
 * 10 mL 1 M TES pH 8.2 (Sigma catalog #)
 * 3 g sodium thiosulfate (Sigma, catalog #)
 * 15 g Bacto-agar (catalog #)
 * 20 mL 50x BG-11 media (Sigma catalog #)
 * 980 mL distilled water
 * a stirring bar


 * Autoclave at 121C for 20 minutes on the liquid cycle.
 * Allow to cool, with stirring, until ~50C or cool enough to handle.

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