IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-16

Running digest products on a gel
Hetmann yesterday digested 10 \xp, 11\sp, and 11\xp. Running them on a gel after CIP treatement of the 11\sp...





Note: Expected values J36011\XP: 550, 3200 J36011\SP: 3700 J36010\XP: 1800, 3200 J36010\SP: 5000

Running diagnostic egel
1: 1kb+ 2: 10 undigested from lastest failed midiprep 3: 10 undigested from the midiprep we KNOW worked 4: 10 undigested from previous failed miniprep 5: 11 undigested from latest failed midiprep 6: 11 undigested from previous failed midiprep 7: 11 undigested from the sequencing miniprep, KNOW worked 8: 11 \xp from today 9: 11 \sp from today 10: 1kb+