User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/15

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04/15/10

 * &#x2713; Minipreps: KAH142, 146, 147/V0201 (20 total)
 * &#x2713; Cultures: 6-hour 2 mL cultures for KAH154-159/MV2 (2 each)
 * &#x2713; Minipreps: KAH154-159/MV2 (12 total)
 * &#x2713; Cultures: KAH142(10), 146/V0201(10)
 * &#x2713; Cultures: V0201 and MV2 (2 each)

Minipreps > Check with XbaI digests

--> 37°C/ ~15 min.

--> Success: KAH147/V0201 --> Pick more colonies for KAH142, 146/V0201

Minipreps 2 > KAH154-159/MV2 > Check with E/P digests

--> 37°C/ ~15 min.

--> Pick 4 more colonies for KAH157/MV2


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