IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct/Design

 Specifications Design Modelling Implementation Testing/Validation  

Registry

 * BBa_T9002
 * [[image:T9002.JPG]]

Design choices

 * This part is already available in the Registry
 * It has the following properties that we need in order to characterize the Prey Sensing Part:
 * The part responds to AHL levels, via activation of pLuxR
 * From this, we can calibrate a AHL assay based on fluorescence
 * LuxR is under the control of pTet, allowing different levels of LuxR to be achieved


 * AHL Levels:
 * The model is sensitive to initial AHL levels
 * As AHL input increase, GFP output increases until saturation
 * The relationship between AHL level and GFP output is described by the equation above
 * The exact relationship can be calculated from this assay

Open issues
A potential issue with the experiment is regarding using pTet as the promoter for LuxR expression. It may be necessary to add a gene for TetR expression to ensure control over this promoter.