IGEM:Cambridge/2008/Notebook/Voltage/2008/07/21

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Aims for Today
The results of yesterday's modified protocol still yielded no growth on the plates. Using yesterday's PCR product our aims were:


 * Cut promoters with spe1
 * Cut RBS with Xba1
 * Ligate these together and perform further PCR amplification of linear fragments.


 * Cut first 'stop' with spe1
 * Cut second 'stop with xba1
 * Ligate these together and perform further PCR amplification of linear fragments.


 * Finally, put all the above elements into a vector plasmid.


 * End of day: Aims completed

Length

 * Promoter = 35 bases
 * RBS = 16 bases
 * Stop (B1002) = 35 bases and Stop (B1006) = 39 bases


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