IGEM:Peking/2007/Switch-Notebook/2007-8-11

colony PCR of T1TE-T1T2-pcc010 and T1T2-pcc010 self-ligation
the results are strange and hard to explain, PCR always tricky???

re-PCR lac, rec, sul, ss1, ss2, ss3, sd1-3
rescue through gel and digest them



colony PCR of yesterday's plate
sula-GFP-plx007



lac-GFP-plx007



ss2-GFP-plx007



re-digestion of GFP-plx007
xbaI and XhoI

in the result, two bands through single enzyme digestion! Two sites for both XhoI and XbaI? seem to be impossible! maybe PCR or other procedure included some other DNA here, we need more thorough check about all these things!!!

positive transformation
to prepare for enough supplies