IGEM:Harvard/2006/vlau/Notebook/2006-6-13

Gel Electrophoresis w/ 2% Gel
1. Protocol - mixed 2g Ultrapure agarose w/ 100mL 1X TBE in plastic flask - heated for 1.5 min in microwave w/ top loosely screwed on   - added 3 EtBr - poured into gel frame and let set for 20 min - placed into running box and submerged w/ TBE - wells loaded - ran gel @ 130V, 45 min 2. Samples L. 1: 1kB ladder L. 2 and 3: DNA nanostructure L. 4 and 5: neg control w/ no oligonucleotides L. 6 and 7: neg control w/ no scaffold L. 8: 1 kB ladder loading dye: BTB dye 50% glycerol + 10X TBE ladder: 10 + 1 dye samples: 10 + 1 dye 3. Image

Transformation P. 2
1. Protocol - prepared 2 liquid cultures for each transformation - added 50 of 5mg/mL amp to 5mL LB per culture - shook @ 180rpm, 37dC overnight