IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/04

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Entry title

 * Lab space preparation.
 * Autoclaved glasswares, tips etc.
 * Obtained protocols for competent cell preparation
 * Prepared media and broth.
 * Prepared different stock solutions (Abs, CaCl, TBE)
 * Working concs of:
 * Amp= 100microgm/ml
 * Kan= 50microgm/ml
 * Chl= 25microgm/ml


 * Stock Solution of 1 M Calcium Chloride prepared.


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