Jennyn/protocols2

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Protocols in development
Please Contribute!
 * Pulse-chase protein production
 * Fixing cells
 * Real-time RT-PCR
 * &beta;-galactosidase assay
 * Denaturing acrylamide gel purification of nucleic acids
 * Quantification of nucleic acids
 * Bacterial species identification
 * Yeast artificial chromosomes
 * One step 'miniprep' method for the isolation of plasmid DNA
 * Cleaning Glass Coverslips
 * "Core Sample" PCR: Amplifying DNA from agarose gels
 * "Broncho-alveolar Lavage
 * Drosophila CHiP on Chip

Adding new protocols
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In vivo
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 * Bacterial cell culture
 * Bacterial Transformation
 * Preparing chemically competent cells
 * Transforming chemically competent cells
 * Electrocompetent cells
 * Electroporation
 * Preparing chemically competent cells (Inoue)
 * Transforming chemically competent cells (Inoue)
 * TOP10 chemically competent cells
 * beta-Galactosidase Assay (A better Miller)
 * Colony PCR
 * Making a long term stock of bacteria
 * Miniprep
 * P1vir phage transduction
 * Recombineering
 * Endy:Pouring plates
 * Protein expression resource
 * S_N Chemostat Protocol

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 * Culture media

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 * Mouse keratinocyte cultures

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 * Cesium Chloride Purification of T7
 * Studier Lysate Prep

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 * Assaying mating
 * Cell Cycle Arrest
 * Dropout plates for yeast
 * High Efficiency Transformation
 * Layered plates
 * Pour YPD plates
 * Silver: Lysate for Western (for Western etc.)
 * Silver: Time-Lapse Microscopy
 * Yeast Colony PCR
 * Yeast DNA Prep


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In vitro
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 * Nucleic acid precipitation
 * Phenol/chloroform extraction

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 * Agarose gel electrophoresis
 * Annealing Primers
 * Designing primers
 * DNA Ligation
 * DNA Synthesis from Oligos
 * Double stranding oligo libraries
 * Library Generation
 * Miniprep
 * PCR
 * PCR Overlap Extension
 * Phosphatase treatment of linearized vector
 * Polony Protocols
 * PNK Treatment of DNA Ends
 * Purification of DNA
 * Reconstituting primers
 * Restriction digest
 * 'Round-the-horn site-directed mutagenesis
 * Sequencing DNA
 * Site-directed mutagenesis
 * Templiphi

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 * In vitro transcription with T7 RNA polymerase
 * Northern Blot, 32P End-Labeled Probes
 * Probe Prep, 32P End-Labeled Probes
 * RNA Extraction
 * RNase Protection Assay
 * RNA Half-life

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 * Acrylamide Gels
 * Silver: Destain
 * Silver: Coomassie Stain
 * Keating:Experimental Protocols:HPLC
 * Silver staining
 * Protein Preps: Yeast
 * Denaturing Protein Extraction
 * Native Protein Extraction
 * Western Blots
 * Pouring Tris-Tricine Acrylamide Gels
 * E. coli Western Blot
 * General Western Blot
 * Purification of His-tagged proteins
 * Free Sulfhydryl Determination
 * Expression and purification of 35S-labeled proteins




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In silico
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 * BLAST
 * CLUSTALW

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 * THREADER
 * Homology Modelling

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 * FBA
 * MoMA

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 * Coot for Model Building


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Other
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 * Flow Calibration
 * Endy:FACS protocols

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 * Design a Microfluidic Chip
 * Order a Microfluidic Chip from the Foundry
 * Operate Microfluidic Chemostat

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 * Agarose Pads for Microscopy
 * Quantitative Microscopy
 * Silver: Time-Lapse Microscopy

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 * Endy:Victor3 plate reader Plate reader usage protocols

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 * Current Protocols in Molecular Biology Downloadable pdfs.
 * Molecular Cloning
 * Protocol-online Useful protocols and a popular discussion section.
 * iProtocol Abridged Current Protocols Invitrogen protocols. Not amenable to printing.
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