Jessica Karen Wong/Notebook/2007-8-4


 * All plates have growth!
 * Set up 10 ul Colony PCR rxns
 * 4 of each I2055-SX-B0032, I2055-SX-B0030, I2055-SX-B0031, I2055-EX-BOO32, I2057-EX-J116, I2057-EX-R0040, I2057-EX-J100, I2057-ES-R0040
 * 8 of each sealed I2055-EX, I2055-SX
 * Made chemically competent MG1655
 * Plated puc18 and 55-SX-B0031 (which grew well on Top10 overnight) as positive controls
 * Transformed 3 different F2620-3K3 ligations in Felix's Top10
 * PCR cleaned the preped I2057-EX E/X and I2057-ES E/S digests
 * Transformed the E0240 Mutagenesis in Top10