User:Shantanu Ghosh/Notebook/Two photon microscope project

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Two-photon microscopy
Two-photon excitation (2PE) microscopy is a form of far-field fluorescence microscopy technique with advantages over conventional confocal microscopy, because of deeper tissue penetration, efficient fluorescence detection and little photodamage. 2PE occurs when two photons of low energy can excite a fluorophore in a quantum event, resulting in the emission of a fluorescence photon, at a higher energy than either of the two excitatory photons. Since the probability of the near-simultaneous absorption of two photons is extremely low, a high energy beam is typically required, usually supplied by a modelocked femtosecond laser.

2PE microscopy was pioneered by Winfried Denk at the Webb Lab at Cornell University.

We are fabricating a two-photon microscope to investigate neuronal plasticity in the auditory cortex.