User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/10

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Primer Dilutions

 * Stock diluted to 100μM
 * Working concentration diluted to 10μM

Reaction
10 μL 5X HF Buffer 1 μL 10mM dNTPs 2.5 μL Forward Primer 2.5 μL Reverse Primer 1 μL GFPuv Plasmid (143.4 ng) 0.5 μL Phusion Hot Start Polymerase 32.5 μL MQ

Validation

 * 0.8% Agarose Gel w/ EtBr
 * Strong band seen & extracted
 * DNA purified with Qiagen kit


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