User:Karmella Haynes/Notebook/miR Trigger/2009/12/31

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12/31/09

 * &#x2713; RNA prep/ cDNA synth.: KAH93, 94, 95/MV2 transfected cells (+/- Dox)

RNA prep & cDNA > 6-well plates: use 1 mL TRIZol per well > Dissolve pellet in 10 μL THE RNA Storage Solution

> Measure RNA concentrations

> oligo(dT) Primer annealing --> details

> cDNA synthesis mix --> 6 reactions total

--> Add 10 μL mix to each annealing rxn. --> 50°C/ 50 min., 80°C/ 5 min., ice --> Add 1.0 μL RNase H, 37°C/ 20 min. --> Store at -20°C


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