IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/07/13

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PCR II

 * Dilute PCR product, PCR again, and gel verify
 * Dilution for each sample: 2uL of PCR product + 10uL of H2O = 12uL (1 in 5 dilution)
 * Protocol: common protocols for PCR


 * MMA: 6 diluted samples + 1 extra + 1 water control = 8 samples
 * MMB: 6 diluted samples + 1 extra = 7 samples

PCR Cycles:
 * 95C @ 2 min
 * Cycle 30x:
 * 95C @ 30 sec
 * 65C @ 10 sec
 * 72C @ 80 sec
 * 72C @ 10 min
 * 10C @ hold

Start: 1225 End: 1355

Gel verification

 * Protocol: in iGEM training manual
 * Changes: Made 1% agarose gel instead of 0.8% gel

Gel orientation:

Machine conditions: 110V, 45 min, 0.5X TBE Buffer Results: Vicki Ma 20:37, 13 July 2010 (EDT)