20.109:W/F Green Team

= Oral Presentation Outline =

Summary
Single nucleotide polymorphisms which are traditionally considered silent (coding for same amino acid) can effect protein folding. We have always been taught to believe that these silent mutations are just that, silent. The fact that they can change protein structure and subsequent function could lead to a much more thorough analysis of DNA mutations and could give us new insight as to how things are mutated.

We want to design a peptide which has several points for silent mutations which are known to create structural differences in proteins. We then want to test the effects of mutating this peptide is humans, yeast and E.coli. Here are the codon biases for these organisms.

Possible Presentation 1
=Background=
 * Drug Pumps, Opiate Addiction, and Parkinsons Disease
 * Fallacy of Silent Mutations
 * Codon Biases
 * Quote on relevance

=Goals=
 * To Test if Translation Kinetics is responsible for protein mis-folding.

=Methods= What do we need to put in eppendorf?
 * Simulate Translation Conditions via Manipulating Codon Bias (in vivo quote)
 * X Ray Crystallography

=Results=
 * Reasons for misfolding

=How can we build on these results?=
 * Drug Delivery
 * Improved Computation Protein Structure Based on shiz