IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/25/Shan Shen

10:00 Miniprep the plasmids containing T7 promoter, standard parts 1-12M and 1-12O. 11:00 Electrophoresis of these plasmids The order and the amount of the samples: marker 10μL, 1-12M 10μL, T7 promoter 10μL, 1-12O 10μL. Results： The circular plasmids present at smaller places of their real size, which demonstrates that the plasmids are normal. 15:30 Single digestion of the plasmids of the T7 promoter to test the efficiency of the single digestion. Digestion System 1: 20μL Digestion System 2: 20μL

16:00 Strart to digest. 20:00 Electrophoresis to test the single digestion. The order and the amount of the samples: marker 10μL,plasmids of T7 promoter 10μL, digesion product of system 1 10μL, digesion product of system 2 10μL. Results: The single digestion product is slower than the cicular ones, suggesting that the single digestion is correct and the efficiency is OK. 21:30 Recycle the fragments. The products of recycling are thrown away by chance by ZHQ.