IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/01/Shan Shen

4:00 Proportion 3:1 is enough for the ligation and CIP is necessary for the double digestion. Choose the plates of double digestion with CIAP to pick the single clones and each plate pick 5 clones. Incubate them at 37℃. 17:30 Mimiprep the plasmids of ligation 20:00 Electrophoresis to test the ligation products The order and the amount of the samples: marker 5μL, plasmids of T7 promoter 2μL, ligation products (1-12M) 2μL, ligation products (1-12O) 2μL. Results: O3 is weird, others are correct. 21:00 Digestion to test the ligation products,Digestion System:20μL 21:50 Start to digest.