IGEM:IMPERIAL/2006/project/critical issues

Open Issues
=Oscillator=


 * The parameters of the oscillator are based on the OD of our 2 cells, their ratio and the volume of the culture.
 * do we want to keep constant the growth ? How ?
 * At which OD do we want the oscillator to operate ? which growth phase (exp, stationary ...) ?
 * Is the use of a chemostat compatible with Lotka-Volterra dynamic ?
 * In which media do we want to use the oscillator (LB, M9) and do the characterization ?


 * Can we have access to Wallac Victor 3 equipement for characterization ? (ask Prof Freemont)