Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization/BRB80

BRB80 buffer consists of
 * 1 mM MgCl2
 * 1 mM EGTA
 * 80 mM PIPES
 * ph 6.9

Cytoskeleton calls this buffer PEM. When you add GTP to PEM, you get GPEM to use Cytoskeleton's lingo.