User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/28

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05/28/11

 * &#x2713; Retransformations: Plasmids for miR Sensor transfections
 * &#x2713; Streak plates: Plasmids for miR Sensor transfections

Retransformations > Measured concentrations of old minipreps, some don't look good. > Retransform 2 μL plasmid (+ 8μL dH2O) into DH5α-Turbo (25 μL) > Spot 10 μL on 1/4 plate (Amp). Streak for single colonies


 * 1) FC1, 12/2/10
 * 2) FC1, mini, 2/25
 * 3) FC2, 12/2/10
 * 4) FC2, mini, 2/25
 * 5) FC3, 12/2/10
 * 6) FC3, mini, 2/25
 * 7) FC4, 3/13/10
 * 8) FC31, 3/10/10
 * 9) FC32, 3/10/10
 * 10) FC33, 3/10/10
 * 11) FC34, 3/10/10
 * 12) KAH118, 1/14/10
 * 13) KAH118, 2/16/10 (Francesca's)
 * 14) KAH119
 * 15) KAH119, 1/15/10 (Francesca's)
 * 16) KAH100, 12/05/09
 * 17) KAH101, 12/05/09
 * 18) KAH121, 12/23/09
 * 19) KAH121, 1/15/10 (Francesca's)
 * 20) KAH122, 12/23/09
 * 21) KAH122, 1/15/10 (Francesca's)
 * 22) KAH124, 12/23/09
 * 23) KAH125, 12/23/09
 * 24) KAH125, 1/15/10 (Francesca's)

--> Re-streak from whatever plates can be found in the cold room


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