NanoBio:FPLC Methods

FPLC Methods

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CAjoFmethodHis

 * Frac-950: serpentine row
 * Variables:
 * Main:Column: HisTrap_FF_5mL
 * Start w/ Pump Wash:
 * Wash Inlet A: OFF
 * Wash Inlet B: OFF
 * Flow Rate: Flow Rate (mL/min): 0.7
 * Column Pressure Limit (MPa): 0.3
 * Averaging Time UV(s): 1.3
 * Eluent A Inlet: A2
 * Start Conc B (%): 0
 * Column Valve: Column_Position: Position 4
 * Column Equilibration: Equilibrate w/ (CV): 1.0
 * Aut_PressureFlow_Reg:
 * System Pump: Normal
 * System Press Level (MPa): 0
 * System Min Flow (mL/min): 0
 * Flowthrough Fractionation:
 * Tube Size (mm):18
 * Fraction Size (mL): 5
 * Flowthrough Fract Start at: First Tube
 * Sample Injection: Empty Loop w/ (mL): 2.0
 * Wash out Unbound Sample: Wash column w/ (CV): 10
 * Fractionation
 * Tube Type Eluate Fraction (mm): 18
 * Eluate Frac Size (mL): 1.0
 * Eluate Fract Start at: Next Tube
 * Tube Type Peak Fraction (mm): 18
 * Peak Frac Size (mL): 0
 * Peak Fract Start at: First Tube
 * Linear Gradient:
 * Target Conc B (%): 100
 * Length of Gradient (CV): 5
 * Gradient Delay: Gradient Delay (mL): 0
 * Clean after Elution: Clean w/ (CV): 2

CAjoFmethodHis1mL

 * Note: same as CAjoFmethodHis except:
 * Main:Column: HisTrap_FF_1mL
 * Fractionation:Eluate Frac Size (mL): 0.25