Kubke Lab:/Notebook/Cranial nerve development/2010/12/02

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Cranial Nerve Development
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=General Entries=
 * 10:30 Went through H&E staining procedure with Satya using our sections cut from embryos Reuben: RC001 and Malisha MH001.
 * 10:45 One slide at a time we practiced the staining procedure -SECTIONS FALLING OFF SLIDE


 * The slides used were clean, unsubbed Original Menzel-Glaser Cut Edges, Frosted Ends. --Reuben Cutfield 18:51, 20 January 2011 (EST)

Decided we definitely need subbed slides. We're going to try using polylysine subbed slides.


 * 11:20 Set cryostat to CT-20, OT-20.
 * 11:45 Began cutting embryo MH001. Mounting on polylysine slides.

SECTIONS CURLING EXCESSIVELY ON PLATFORM AND PINCHING AT EDGE OF GLASS

=Personal Entries=

Reuben

 * 1:00pm Lowered temperature to Ct-24,OT-24, waiting to equilibrate
 * 2:00pm Began cutting again using embryo MH001 mounting onto polylysine slides.


 * 2:30pm Grabbed another embryo and staged it as stage 20 and labelled as RC002 --Reuben Cutfield 18:25, 7 December 2010 (EST)

Malisha
RESULTS STILL POOR. OCT was rubbery therefore we decided to start with a new embryo--Malisha Hettiarachchi 21:25, 12 December 2010 (EST).

Staged an embryo labelled "stage 28" to confirm and labelled as MH002--Malisha Hettiarachchi 21:25, 12 December 2010 (EST)


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