Kubke Lab:/Notebook/Cranial nerve development/2010/12/16

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=General Entries= Meeting with Malisha regarding the planning of the work around the christmas holidays and the new building works schedule --MF Kubke 18:02, 15 December 2010 (EST)

Cryostat sectioning
MH004 Tried a few settings on the cryostat. Based on unstained check under the microscope, I seem to have gotten good quality by changing the knife angle to 0 - cut sections between 25-40 microns at 17 C. Tried colder but got a feeling they are more cracky, so stuck with 17C.--MF Kubke 23:20, 15 December 2010 (EST)

=Personal Entries=

Malisha

 * 9.20 AM: Nissl stained slide number 3 and 9 from MH003 . Two minute dehydration and 1 minute rehydration. The slides were left in Xylene for 15 minutes and left in the crysl violet for 5 minutes. Results: did not cover slip so the color was whitish. Will restain with the rest of the slides. Tissue structure was destroyed in slide 3 but intact in slide 9.

- This has been done --Malisha Hettiarachchi 21:06, 3 February 2011 (EST)


 * 11.10 AM: Staged MH004 to be in between stage ST28 and ST29. A dissection was made the wing bud and the rostral region was made into a mould. Currently it is incubating at -17 CT/OT. Reuben will continue the process and begin cutting. --Malisha Hettiarachchi 18:18, 15 December 2010 (EST)

Fabiana

 * Tested cryostat settings (see General Entries/Cryostat sectioning above)

Reuben
The afternoon was spent observing Fabiana's sectioning of MH004 alongside Malisha. Meanwhile I also performed a Nissl stain on slides 5 & 4 from embryo MH003 (incorrectly labeled as MH-004) which had been mounted on Tuesday afternoon by Malisha. Slides 5 and 4 are currently stored in the slide folder labeled RC003.

I have moved these slides into MH003 folder. Thanks for the note reuben--Malisha Hettiarachchi 20:58, 3 February 2011 (EST).


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