User:Lu Wang/Notebook/Team Allergy/2010/07/28

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Protocol
Yesterday, we plated E. coli containing complete hpRNA for Ger, Bet v2, and LTP. Bet v1 did not ligate properly in the earlier Sense+Intron ligation.

Today, we will extract the plasmids from the E. coli and prepare them for sequencing tomorrow. We will also do ligations with the false negatives (Sense + PDK ligate with Antisense).


 * 1) Culture colonies
 * 2) Miniprep
 * 3) Nanodrop
 * 4) Digest false negatives
 * 5) Ligate
 * 6) Transform

Results
Nanodrop

The format is: Miniprep #, Name of Allergen, concentration in ng/μL of colony 1, concentration in ng/μL of colony 2. All these working ligations are with the PAL intron


 * 9, LTP, 97.9, 94.1
 * 11, LTP, 77.9, 94.8
 * 25, Bet v2, 67.8, 97.8 (there was a mixup with colony 1 and LTP+PDK, but it is now fixed in our electronic notebooks)
 * 28, Bet v2, 73.2, 83.6
 * 36, Ger3, 76.1, 83.4
 * 37, Ger3, 70.0, 75.3
 * 38, Ger3, 90.4, 108.3


 * Transformed more sense+pdk parts for our false negatives (16,30,31)


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