Bitan:todo

these are experiments that the wiki does not yet have a protocol for

=Experimental Procedures=

Aβ preparations

 * HFIP Film Preparation
 * Soluble Oligomers
 * Microwave-assisted peptide synthesis
 * PICUP
 * SATA conjugation to Aβ

Molecular Biology Methods for SELEX

 * qPCR
 * RNA biotinylation
 * PCR Amplification of the Initial ssDNA Library for SELEX experiments
 * Agarose gel electrophoresis
 * Measuring DNA concentration by absorbence
 * In-vitro transcription, labeling and G-50 purification of RNA
 * Calculating specific activity of hot CTP after calibration date
 * Scintillation counting using the Triathler bench-top counter
 * TBE-urea-polyacrylamide gel electrophoresis and autoradiography
 * Calculating the yield and specific activity of labeled RNA
 * Filter binding for SELEX
 * Extraction of RNA from filters
 * Autoradiography of hot blots
 * Autoradiography of hot gels

Structural Studies

 * Electron Microscopy
 * CD
 * ThioflavinT fluorescence
 * Dynamic Light Scattering

Protein Analysis

 * ALISA
 * Western Blot
 * ImageJ densitometry
 * dot blot
 * ELISA/ELONA/α-syn-ELISA-Total/α-syn-ELISA-oligo
 * Biotinylation of mAb211

Cell Biology

 * Cell Culture


 * LDH assay


 * MTT assay


 * Isolation of primary cells


 * Extraction of mice brains for α-syn ELISA

Other amyloidogenic proteins
Aggregation (ThT Fluorescence & EM)
 * α-syn
 * β2m
 * Calcitonin
 * Insulin
 * Transthyretin
 * Prion
 * IAPP
 * SOD1
 * IAPP

Fibrils

Toxicity
 * α-syn
 * β2m
 * Calcitonin
 * Insulin
 * Transthyretin
 * Prion
 * IAPP

Some Useful Software Usage Tips

 * EndNote
 * Microsoft Word

=other pages we need= Guidelines Lab Members Bitan:Research Publications Bitan:Talks

[Back to Bitan page]



       Molecular biology

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