User:Yoonseok Jung/Notebook/Polycomb Project/2011/02/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Entry title
Insert(5XGAL4) | Vector(Spacer) DNA   25uL    | DNA     10uL 10X FD 3uL    | 10X FD   3uL EcoRI  1uL    | EcoRI    1uL SpeI   1uL    | XbaI     1uL | dH2O   15uL (30uL) |        (30uL)
 * DNA Digest

37C/15min


 * Gel synthesis

1. 1X TAE 60mL + 0.7g Agarose 2. 40s microwave, shake a flake, and 40s microwave again 3. Add 6uL EtBr




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