User:Vijay/Notebook/2007-2-12

Fusion

 * 1) Plasmid prep from phoA/lacZ (pBAF/pLKC480) and gel. Done
 * 2) Double digest phoA/lacZ and gel. Done
 * 3) 8 &mu;l vector
 * 4) 2 &mu;l buffer
 * 5) 1 &mu;l enzyme
 * 6) *EcoR1 for 1hr at 370C, followed by Sal1 for 1hr at 370C in cycler
 * 7) 9 &mu;l water
 * 8) Stock phoA/lacZ. Done

Fractionation

 * 1) Inoculate UAMS-1 wt for 2nd fractionation. Done