FLP

The 2micron plasmid in Yeast ''Sacharomyces cerevisiae ''

=General=
 * each yeast cell contain about 100 copies of the 2micron plasmid
 * 2micron plasmid replicate autonomously
 * exist in 2 isomers inside each cell, each about 50%.

=FLP=
 * FLP invert a sequence between 2
 * FLP mediated recombination plays an important role in plasmid amplification
 * Excessive amplification is toxic for the cell, so FLP is tighly controlled

=FRT site=
 * 3 repeat of 13 bp (a, b, c)
 * 8bp (AT)-rich core region, with assymetric sequence, dictate directionnality.
 * assymetric distribution and orientation of abc sites arround the core sequence:

'''-c-→ -b-→ ≤core≥ ←--a--- '''