IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 RNA Decoder/2010/06/29

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Entry title
Did a Digestion today of pSB1AT3 to test our digestion enzymes. We cut pSB1AT3 with SpeI+XbaI, SpeI, and XbaI. To do this procedure we used:
 * RXN 1 (SpeI)
 * SpeI 1 uL
 * Buffer (2) 5 uL
 * BSA .5 uL
 * DNA (pSB1AT3) 1.5 uL
 * dH2O 42 uL
 * Total= 50 uL
 * RXN 2 (XbaI)
 * XbaI 1 uL
 * Buffer (2) 5 uL
 * BSA .5 uL
 * DNA (pSB1AT3) 1.5 uL
 * dH2O 42 uL
 * Total= 50 uL
 * RXN 3 (SpeI + XbaI):
 * SpeI 1 uL
 * XbaI 1 uL
 * Buffer (2) 5 uL
 * BSA .5 uL
 * DNA (pSB1AT3) 1.5 uL
 * dH2O 41 uL
 * Total= 50 uL

We then ran a gel of the 3 reactions on 1% agarose gel.