Jacobs:Back Door

Useful Links / Meetings

 * Chemtracker
 * ORS
 * ASME SBC
 * ASBMR
 * BMES

Equipment and Schedules

 * Wish List
 * TC Hood Schedule
 * Lab Meeting Schedule
 * Lab Jobs
 * Freezer Defrost Schedule

Research Protocols

 * Safety
 * Freezing and Thawing Cells
 * Actin Staining
 * Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
 * Protein Gel Staining Using Coomassie Blue Protein Stain
 * Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
 * Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
 * Total Protein Isolation Using RIPA Lysis Buffer
 * Transfecting NIH 3T3s with GFP-Actin
 * 10 bp DNA Ladder
 * Collagen Gel Contraction 2
 * Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
 * Loading Mice
 * OFF (Large Chambers)
 * Flow chamber calibration
 * siRNA transfection
 * Separating and Tagging of Mice
 * Genotyping Tailsnips
 * Tubulin Immunostaining Protocol
 * Confocal Imaging of Flow
 * Cell Culture numbers
 * Troubleshooting Cell Culture
 * Lecia MP Microscope Protocol
 * In vivo microCT scanning protocol for live mice
 * Isolation of Primary Bone Cells
 * Jacobs: Cell Culture of MLOY-4 Osteocyte Cells
 * Jacobs: Cell Culture of IMCD Kidney cells
 * Cell Culture of MDCK cells
 * Autoclave
 * Alkaline Phosphatase Assay
 * Neon Transfection
 * Coating Slides with Fibronectin
 * Osteocyte Markers
 * Printers
 * Immunostaining for TRPV4 and PC
 * Preparation of primary osteocytes and MDCK cells for immunostaining

Teaching Protocols

 * BCA Total Protein


 * Adipocyte Differentiation


 * Oil Red O Staining


 * RNA Agarose Gel


 * Collagen Gel Contraction


 * Real-Time PCR


 * Pipetting Exercise


 * Total Protein Isolation using RIPA buffer


 * Total Protein Isolation using RIPA buffer 2


 * RNA Sample Loading Buffer


 * Total RNA Isolation using Tri Reagent


 * Subculturing 3T3 Fibroblasts


 * NuPAGE Electrophoresis and Western Blotting for Proteins

Miscellaneous

 * Photos:Jacobs photos
 * Movies