Template:SBB-Protocols PCR3

SOEing PCR

 * Set up PCR reactions according to your construction file as normal 33uL reactions as described in Cloning by PCR
 * For each PCR, load 6uL of PCR product premixed with 4uL of loading buffer in a single well of a 1% agarose gel
 * Cut out the bands, put them into a single 1.5mL microcentrifuge tube
 * Add 650uL of ADB Buffer
 * Proceed with the Zymo Gel Purification procedure
 * Elute the DNA in 50uL of water
 * Set up your second round of PCR as a normal 33uL reaction using the eluted mixture of fragments as template