User:Fermenter User/Notebook/BROM C7

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM C7

Day 0 (Apr 20 Tue):
            4/20/2010 (11:30) Bring media to fermenter room            4/20/2010 (13:00) Assemble bioreactor 4/20/2010 (13:00) Add 5 ml of Antifoam/fermenter 4/20/2010 (13:50) Start Sterilization (Liq 45 min) 4/20/2010 (15:30) Cooling of bioreactor (Temp setup) 1 L/min air, 300 rpm 4/20/2010 (15:44) Start Fermentation Software for Fermenter #6 4/20/2010 (15:46) Start Fermentation Software for Fermenter #7 4/20/2010 (16:00) Connect Acid (250 ml) and Base (400 ml) Start pH control 4/20/2010 (16:10) Calibrate dO2 probe with 300 rpm, Air purge for 30 min 4/20/2010 (16:10) Set dO2

            4/20/2010 (16:30) Inoculation: inoculation volume (300 ml) Fermentation 0:00 F6: Cell weight (?? mg), Activity (23%, x100), Activity (9%, x1000) F7: Cell weight (?? mg), Activity (37%, x100), Activity (23%, x1000)

Day 1 (Apr 21 Wed):
           4/21/2010 (9:30)  Increase rpm to 500 Fermentation 16:00 F6: Acid (250 ml) Base (230 ml) F7: Acid (210 ml) Base (230 ml)

            4/21/2010 (11:00) Sampling  Fermentation 17:30 F6: Cell weight (?? mg), Activity (97%, x100), Activity (74%, x1000) F7: Cell weight (?? mg), Activity (97%, x100), Activity (79%, x1000)

            4/21/2010 (14:30) Sampling  Fermentation 22:00 F6: Cell weight (?? mg), Activity (96%, x100), Activity (74%, x1000) F7: Cell weight (?? mg), Activity (95%, x100), Activity (82%, x1000)

   4/21/2010 (15:00) Fermentation 22:00 Harvest Fermentation F6: Acid (250 ml), Base (180 ml) F7: Acid (210 ml), Base (140 ml)


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