User:Jeffrey Kim/Notebook/High-throughput metagenomic profiling of secondary metabolites

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High-throughput metagenomic profiling of secondary metabolites

 * Touchdown Phusion protocol

98C 30s

98C 10s 72C 30s 72C 30s (7x TD -2C/cycle) 98C 10s 58C 30s 72C 30s  (29x)

72C 4 minutes

Standard phusion

98C 30s

98C 10s ann 30s 72C 30s

72C 5 minutes

(AD = 72C annealing),