IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/14/Shan Shen

12:30 PCR to test GFP

15:30 Electrophoresis to test the PCR products. The order and the amount of the samples: marker 5μL, clone 1 10μL, clone 2 10μL, clone 3 10μL, clone 4 10μL, clone 5 10μL. The band appeared at about 1kb seems to be the correct products.

16:00 PCR bi-stable to insert into the chromosome.

20:30 Electrophoresis to test the PCR products. The order and the amount of the samples: marker 5μL, bi—stable PCR products, plasmid control of low-copy bi-stable system, digestion products of low-copy bi-stable system. Results: PCR and digestion are both unsuccessful.