IGEM:PennState/Labbook/NoahJohnson/2007-8-10

August 10, 2007
 * Luc did miniprep on P1010
 * I ran a digest with SpeI and EcoR1 again
 * I used PCR tubes this time and ran 20uL digests
 * Added to tubes:
 * 4,8,and 12uL DNA
 * 2uL EcoR1 buffer
 * 2uL BSA
 * 0.5uL each restriction enzyme
 * dH2O to yield final volume of 20uL


 * Started incubating at 12:50pm

10-4