IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/10

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9/10/10
10:15 AM

Re-Ran yesterday's PCR

Verification PCR

Samples:   B0034ArsRSDM2B0015pSB1C3   negative control DI H2O  

Primers: VF2, VR, 20 micromolar

Program:

2:05 PM

Gel

1%, TAE, 175 V



2:45 PM

PCR Cleanup

B0034ArsRSDM2B0015pSB1C3: 23.9 ng/μL

Set up sequencing reactions:

4.5 μL DNA 6 μL VF2 (10 micromolar) 19.5 μL H2O


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