Koch Lab:Inventory/Coherent 1064nm DPSS/June 2009 email

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 1064 4W Coherent laser / OT Plan  

Use this wiki page for recording information on laser<SPAN LANG="en-us"><FONT FACE="Calibri">:</FONT></SPAN><SPAN LANG="en-us"> </SPAN><A HREF="http://openwetware.org/wiki/Koch_Lab:Inventory/Coherent_1064nm_DPSS"><SPAN LANG="en-us"><U><FONT COLOR="#0000FF" FACE="Calibri">http://openwetware.org/wiki/Koch_Lab:Inventory/Coherent_1064nm_DPSS</FONT></U></SPAN><SPAN LANG="en-us"></SPAN></A><SPAN LANG="en-us"><FONT FACE="Calibri"></FONT></SPAN><SPAN LANG="en-us"> </SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">Hey All,</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">Evan has loaned us the 4 Watt 1064nm Coherent laser to use for our tweezers in the near term</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">. Igor and I moved it over to the</FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">&#8220;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">new lab</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8221;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> yesterday, along with some other optics. I</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">m trying to get a lot of the junk moved off that table today (hopefully Dan Bryant will be in to help do that).</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> Once much of the junk has been moved (all big old lasers,</FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">except</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> for one, all the shelves should b</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">e clear) we can finish moving equipment over, and construct the tweezers</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">.</FONT></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Symbol">&#183;<FONT FACE="Courier New">     </FONT></FONT></SPAN><SPAN LANG="en-us"></SPAN><SPAN LANG="en-us"></SPAN><SPAN LANG="en-us"></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">There is an issue with image acquisition / MT work that I</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">ll send another email about</FONT></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">Here is what I think should be done</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">, up until the point of having a trapped microsphere</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">. Please</FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">do chime in if you</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">d like to participate in anything which I</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">ve not assigned. The intention is to be happy and productive, not to exclude anyone.</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> If you</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">d like to participate in one step or another, that</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">s fine to do so planning amongst yourselves.</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">  As I</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">ve written it, I haven</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">t listed tasks for Ant or Brigette, which doesn</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">t mean anything more than</FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">they</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">re doing other things not listed.</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">1. </FONT></SPAN><SPAN LANG="en-us"></SPAN><SPAN LANG="en-us"></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri"> Moving &amp; Cleaning</FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">&#8211;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> Linh</FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">can lead this, along with Andy to the extent he</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">s able. I can also help. Moving microscope, little optical table, kochlab-daq2, and associated necessary optics. Light cleaning of big optical table and shelves</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> if necessary.</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">2. </FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">Setting up 1064 nm laser (cleaning / installing fiber). Steve &amp; Igor &amp; Andy</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">3. </FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">Characterizing 1064 nm laser. Linh can lead this. Using ND filters and cheap CCD camera / beam chopper,</FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">we need to know:</FONT></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">a. </FONT></SPAN><SPAN LANG="en-us"></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">Beam power (thermopile)</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">b. </FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">Beam diameter at specific location (centimeters) relative to output coupler of laser.</FONT></SPAN><SPAN LANG="en-us"> </SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">c. </FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">Beam divergence</FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">&#8211;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> beam chopper or CCD (probably CCD) measuring at several points downstream to calculate divergence angle.</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">d. </FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">Expansion characteristics of the fixed and variable beam expanders.</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"></FONT></SPAN><SPAN LANG="en-us"> </SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">4. </FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">Designing OT path</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8212;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">Larry. Using parameters from #3 above</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> and known info about back apertur</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">e, 1064 nm optics we have, and beam expanders</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">5. </FONT></SPAN><SPAN LANG="en-us"> <FONT FACE="Calibri">Aligning OT</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8212;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">Andy and Igor.</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> Trapping free 0.5 micron microspheres.</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">After trapping in step 5, we</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">&#8217;</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri">ll regroup and make another plan for calibration and DNA stretching.</FONT></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">Definitely please let me or the dna</FONT></SPAN><SPAN LANG="en-us"><FONT FACE="Calibri"> email list know if you see problems with this plan!</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"></SPAN></P>

<P DIR=LTR><SPAN LANG="en-us"><FONT FACE="Calibri">--S</FONT></SPAN><SPAN LANG="en-us"></SPAN></P>

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