User:Tk/Notebook/MF/2009/11/09

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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Growth of L1, GF, PP strains

 * Colony sizes at 38 hours
 * L1: 14-16 div
 * GF: 11-14 div
 * PP: 8-11 div Morphology of the PP colonies is peaked, with much less spreading of the outside, higher growth on the central region.


 * original W12 plate shows some mycoplasma shaped colonies, but also yeast? contamination


 * No growth visible on sterile plate or on the newly streaked (14 hour) W12 plate

Growth at 19 hours of inoculated liquid cultures

 * L1 B clear yellow
 * L1 A slight orange
 * GF A slight orange
 * others unchanged


 * Still no growth on newer W12 plate
 * put L1, GF, PP plates at +4 in bags

Growth at 22 hours

 * L1 A clear yellow
 * L1 B yellow, slightly cloudy
 * GF A yellow-orange
 * GF B yellow-orange

Made 1161 medium and plates, made 1161 agar stabs.

Growth at 24 hours

 * L1A, L1B slightly cloudy
 * GFA, GFB yellow
 * four cultures put at +4


 * Still no growth in PP cultures, W12 new plate
 * Picked two more colonies from PP plate, PP C, PP D
 * Picked two colonies from W12 original older plate, W12 A, W12 B


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