IGEM:Brown/2008/Notebook/Team Resistance/2008/06/08

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Team Resistance

 * Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution.

Considerations for gene cassette
Harvard’s paper

•	Used luria lb

•	Grew cells up to mid-logarithmic stage then added the arabinose

•	Saw decrease in cell density after just 1 hour, but no change in the control

•	Used plasmid pvj4

•	E.coli strains: DH5alpha Beta2155

•	Can we check tightness of promoters by using gfp?

Materials for lab:

More 1000ml wheaton bottles to be able to store lb (possibly borrow)

Requested meeting with professor palmore

Questions

•	What kind of things affect resistance (in terms of our apparatus)

wires slipped through cardboard

•	distance between each wire in plates 0.25 in. and 1.73 in. into plates


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