User:Anthony Salvagno/Notebook/Research/2009/01/29

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Biolab Prep
Some Emails
 * My emails that started it all:
 * Hey (Person), I’m not quite sure how I should format this email, but I am interested in working with you to learn the techniques of ligation that Brandon has learned over the past several months.  I wanted to run it by you and Koch will be over soon to talk with (other person) and yourself about this possibility as well.  I can be in the lab rather frequently, and am very excited about pursuing this with you.  Hopefully I can get some positive results and even work with you on other things.  Anything I can learn will be a huge benefit and hopefully I can return the favor in contributions to your work.
 * Hey (person), I’m following up from a couple weeks ago.  Koch told me I should wait a little while to give you some time to take care of other important things.  Anyways, if you are ready to have me I am ready to join the lab.  When would be the best time to meet and go over the initial planning phases?  Is there any literature I should read to get acquainted with some terminology, procedures, etc?
 * /Person's Email
 * /Koch's Email

Things to read

 * Read up on some ligation protocols
 * Kochlab protocols, Diego's Notebook, Brandon's notebook
 * Maybe Koch grad school notebook
 * learn about plasmid DNA
 * check out NEB site: NEB
 * check out molecular cloning books
 * Learn about:
 * multiple cloning sites
 * T4 DNA ligase and ligation reactions
 * agarose gel electrophoresis
 * miniprep (plasmid DNA isolation from E. coli)
 * read pipette manual (find it too).


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