IGEM:PennState/Labbook/GalenLynch/2007-7-11

July 11th, 2007: 9:30-5:30 Quick Change to strengthen Xyl promoters XylA to XylF dual promoter/operator BBa_I741015 GGTGATGGAT GA T GTCGT A T  TAT  A GGGCAC TCCCTTTCAG TT TG  TCA  A  TT ATGTTATTTC ACA CTGCT AT TGAGATAATT CAC A  AGTGTG CGCTCGCTCG CA AAATAAAA TGGAATGATG AAACTGGGTA ATTCCTCGAA GAGAAAAATG CAATAAGTAC AATTGCGCAA CAAAAGTAAG ATCTCGGTCA TAAATCAA GA AATAAACCAA AAAT CGTA AT CGAAAGATAA AAAT CTGTAA TTG ACA TCCC CTGTTTAGTT GC TA  T  AA  T TT GGTT A CGTTT ATCGCGGTGA TTGTTACTTA T Color key: Transcription +1 is in maroon, promoter regions are in green , XylR binding sites are in blue , CRP binding sites are in purple , shared promoter and XylR binding sites are in teal , shared XylR binding sites and CRP binding sites are in indigo , bases modified from wild type using quick change are in orange Quick Change Primers