User:Maximilian Peters

Contact Info



 * Maximilian Peters
 * Hebrew University of Jerusalem
 * Email me through OpenWetWare

I learned about OpenWetWare from google, and I've joined because Learn and contribute.

Site directed mutagenesis protocol
http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis

Pharmacology of TRP channels
List of antagonists and agonists of TRP channels

Protein-Protein interaction prediction servers
http://www.compbio.dundee.ac.uk/www-pips/ http://crdd.osdd.net:8081/ProPrint/submit.jsp http://www.russell.embl.de/cgi-bin/tools/interprets.pl

Publications

 * 1) Paper1 pmid=17478614
 * 2) Paper2 pmid=17712814
 * 3) Paper3 pmid=17714026
 * 4) Paper4 pmid=19135721
 * 5) Paper5 pmid=19535910

Useful links

 * Introductory tutorial
 * OpenWetWare help pages

Good enyzmes for cutting PCR products close to the end
AflIII: CCACATGTGG AscI: GGCGCGCC AvaI: CCCCCGGGGG BamHI: CGGGATCCCG ClaI: CCATCGATGG EcoRI: GGAATTCC HaeIII: GGGGCCCC KpnI: GGGGTACCCC StuI: AAGGCCTT XbaI: GCTCTAGAGC

Criteria: >90% digestion in 2h, short sequence http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp

Solutions and Media
List of different stock solutions Schneiders S2 medium:

Reference: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/schneiders_insect_media.Par.0001.File.tmp/schneiders_insect_media.pdf

DMEM - Dulbecco's Modified Eagle's Medium

With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine

Reference: http://www.cellsoup.com/cmandrfr.html

RPMI 1640 with Sodium Bicarbonate, without L- Glutamine,

Reference: http://www.cellsoup.com/cmandrfr.html