User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/26

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] SDS-PAGE & Refreshed medium
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Run 8% gel with lysate CTR/CSE 2
 * CSE|CTR|Marker|CTR|CSE
 * Prepare 10x Semi Dry Buffer
 * 48 mM TRIS (58.2 g)
 * 39 mM Glycin (29.3 g)
 * 0.0375% SDS (18.8 mL of 20% stock)
 * Up to 1 L (no pH change necessary)
 * Cells in 10cm dishes were not confluent and refreshed with S+


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