IGEM:MIT/2005/Tabled because we do not have ToxR-cI fusion: Signal Test (see: John Mekalanos, Harvard, ToxR-cI Fusion)


 * scFv-ToxR-cI-Reporter fusion
 * Check for fusion of scFv doesn't cause dimerization or block dimerization
 * Then test for different length linkers of fluoreceins. (can be synthesized for us invitro)
 * Which one cause transcription of reporter? (test constructs)
 * ToxR co-operative interactions are not modulated by environmental conditions or periplasmic domain conformation, Michelle Dziejman
 * When ToxR dimerizes, the proteins attached to ToxR can bind to DNA to bring it together or not.
 * Can see BetaGal or not.
 * (plates) Xgal -> "x" = blue + galactose
 * (liquid) ONPG -> ONP (yellow A420) + galactose
 * Parallel: build ctx promoter with reporter.
 * We can test this now. We just need the plasmid and strains..
 * Does it autodimerize? Figure out natural behaviors

People

 * Michelle Dziejman

../From email of 7/7 to NK/