IGEM:IMPERIAL/2006/LabCalendar/10aug06

Lab Update 10th August
This Report is a summary for full details see the lab notebook

Aims For Friday

 * Finish Stage three Ligations (With the exception of the aiiA part)


 * Finish Stage two ligations (by wedensday)


 * Test part T9002 (This part is the AHL/GFP assay and the Predator AHL sensor)

Aims For today

 * Maxiprep and electroporate for ligations John SY
 * Retry PCR Cre Lox part John C
 * PCR Immunotag Deepti and johhny

Current Ligation Status

 * Tick = Done and tested
 * ?   = Ligated and untested
 * X   = Done and Failed we will retry
 * [[Image:Ligation Chart14aug 2006.ppt]]

Issues

Riboswitch


 * Unable to tell if worked from PCR
 * Will be sequenced (takes 2-3 days) waiting on result

PCR


 * The PCR results do not correlate with the miniprep results
 * PCR for immunotag and lox part is not working

minipreps


 * These are not conclusive for many parts, we are looking into using a restriction digest

John Chattaway (Lab Co-ordinator)