IGEM:UBC/2009/Notebook/Cre excision frequency/2009/08/15

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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ML6 digestion

 * Restriction digest started @ 10:14am to 11:15am.
 * heat inactivated @ 11:16 to 11:38.

ML6 ligation

 * ligation (16oC)@ 11:40 to 2:40pm.
 * was left in thermal cycler at 4oC until 3:50pm.

ML6 transformation

 * ligated product introduced into DH5a competent glycerol cell @ 4:08pm
 * on ice from 4:08 to 4:38pm
 * heat shocked for 1 min and on ice for 2 mins(in span of 4:39 to 4:42)
 * recovery from 4:42 to 6:44pm
 * spread plated @ 6:47pm
 * plate was left in Biosafety cabinet to dry for 20 minutes.
 * incubation started at 7:11pm.

Other stuffs

 * made LB agar and autoclaved it.


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