Freimoser:Recipes:Yeast:YNB vitamins

=1000x vitamin solution for yeast nitrogen base=

Remarks:
The recipe described here is based on the description from the | John McCusker lab. In contrast to the original recipe I have dissolved pyridoxine and thiamine in water instead of ethanol. Therefore I have added 4 ml EtOH to the ethanol-soluble vitamins in order to keep the volume the same. I have not checked if it is necessary to assure that all vitamins can be dissolved.

Mixing
Volumes for the preparation of 50 ml of a 1000x vitamin stock solution.


 * 1) remove the individual stock solutions from the refrigerator, may have to warm to get them back into solution
 * 2) combine all 95% EtOH vitamin solutions (2 ml), add 4 ml 95% EtOH
 * 3) Add 10 mg riboflavin 5' monophosphate (sodium salt dihydrate) to 95% EtOH mixture
 * 4) Combine the filtersterilized, water-dissolved vitamin solutions (11 ml) with 33 ml sterile H2O
 * 5) Combine the sterile H2O solution with the 95% EtoH solution/suspension for a final volume of 50 ml.