User:Anthony Salvagno/Notebook/Research/2010/06/25/Tethering in 3 different Environments

Big beads are a bitch, and I'm going to make them mine!

The Quest
I need to get tethering to work. Period. So I will try tethering in 3 different ways: This should be fun. Each sample will have a no DNA sample to keep tabs on.
 * 1) Tethering with the beads in just Popping Buffer (not BGB)
 * 2) Tethering with BGB beads at 37C
 * 3) Tethering with BGB beads at 4C

The Prep

 * 1) Wash small amount of beads (4k*g for 5 min), remove supernatant, resuspend in 50ul 1x Pop
 * 2) Dilute anti-dig 1:100 in PBS
 * 3) Dilute TpBR 1:100 in 1x Pop
 * 4) Make sample chambers
 * 5) Tether