Template:SBB09 15954

Dirk van Swaay

Beta Roll
Source: E. coli O157:H7 Genomic DNA, or similar You are going to make a passenger part encoding a beta roll. For background, read PMID: 8253063 and PMID: 2839840. The nucleotide sequence of the source gene is at locus ECOHLY, but you aren't going to clone the entire CDS.

The region you want to include in your part is  tattccgtggaagaacttattgggaccacgcgtgccgacaagttttttggcagtaaatttgctgatatcttccatggcgcggatggtgatgaccatatagaaggaaatgacgggaatgaccgcttatatggtgataaaggtaatgacacactgagtggtggaaacggagatgaccagctctatggcggtgatggcaacgataagttaattgggggagcaggtaataattacctgaacggcggagatggcgatgatgagcttcaggttcagggaaattctcttgcaaaaaatgtattatccggtggaaaaggtaatgacaagctgtacggcagtgagggagcagatctgcttgatggcggagaagggaatgatcttctgaaaggtggatatggtaatgatatttat

The translation of your final part (excluding the BamHI and BglII sites should be: YSVEELIGTTRADKFFGSKF ADIFHGADGDDHIEGNDGND RLYGDKGNDTLSGGNGDDQL YGGDGNDKLIGGAGNNYLNG GDGDDELQVQGNSLAKNVLS GGKGNDKLYGSEGADLLDGG EGNDLLKGGYGNDIY Note that there is an internal BglII site in this sequence that must be properly removed.

Silver-Binding Peptide AG4
Source: Synthetic For background, read PMID: 12618805. Make a BglBrick part encoding the peptide: NPSSLFRYLPSD no start, no stop, in frame.

Cellulose Binding Knottin
Source: Synthetic You will be making a cellulose binding peptide. For background, start with PMID: 2554967. Your part should encode: GSgptqshygqcggigysgptvcasgttcqvlnpyysqclGSG This sequence does not include the amino acids encoded within the BglBricks polylinker. Your part should have no start, no stop, and be in frame.