SBB10 protocols

All these protocols in one big list

Polymerase Reactions
Wobble Reaction Cloning by PCR SOEing PCR PCA Gene Synthesis QuikChange

Cleanup Reactions
Regular Zymo Cleanup Small-Frag Zymo Cleanup Zymo Gel Purification

Digestions Reactions
EcoRI/BamHI Digest of Wobble Products EcoRI/BamHI Digest of PCR Products BglII Digest of EIPCR Products Ligation of EcoRI/BamHI digests Ligation of EIPCR digests Analytical digests (Mapping)

Bacteria Manipulations
Transformation by heat-shock Picking of colonies Miniprep purification of DNA

Other
LR Gateway Transfers EcoRI/BamHI Part Transfers What do I do with my sequencing data? Small Scale Competent Cell Transformation ELISA