ISISBio:Internal/Lab Supplies/old

Tues 26th August

 * ELGA UHQ5 RO cartridge, Cooling Fan, 1/2 day labour Quote WQ005499

Thurs 21st Aug

 * New England Biolabs BlpI R0585S 500 units 10,000 units/ml
 * Agilent - XL1 Blue Competent Cells 5 x 0.2ml ordered 2 packs
 * Promega BamH1 restriction enzyme
 * Fisher 5ml pipette tips
 * Jencons Jenway pH Meter expected end of August
 * 100pk 0.22um 25mm filter membranes

Weds 20th Aug

 * BioRad Precision Plus Protein Kaleidoscope standards x2

Date unknown

 * Bugbuster - ===arrived?===
 * Benzonase (from Novagen as well as bugbuster) ===arrived?===
 * Glucose (if we don't have some but I don't think we do) ===arrived?===


 * Fisher kilobase marker x2 one in stock one to follow
 * Fisher 25-500bp marker x2
 * Fisher Invitrogen Kanamycin sulphate 25g
 * NdeI and NaeI (Promega R7131 and R6801)
 * Merck dodecyl maltoside 25g

Mon 11th August 2008

 * Lyreco 10x Arch files for Louise's office R78
 * Whiteboard markers 1 x 4pk
 * Whiteboard cleaning and accessory kit
 * A4 ruled manuscript books x5

Fri 8th August 2008

 * Fisher by requisition fume hood and cabinet


 * items ordered Weds 6th August
 * Fisher Sodium Acetate 500g
 * 10ml syringes
 * 30ml Syringes
 * Paper rolls for lab
 * Fisher 25g Arabinose
 * 10pk 500ml Durans
 * 10pk 1L Durans
 * 10pk 250ml Durans
 * 5x 1ml Histrap Ni columns
 * ordered weds 30th July 2008 25g dodecyl maltoside (DM)
 * Weds 9th July 2008 arrived Heraeus Megafuge 11R, 230V Ex-Demo unit s/n 306090617 Delivery expected 11/08/08
 * T41 Swing out rotor
 * Set of 4 buckets for T41 rotor
 * Sealing lids for T40 buckets
 * Insert, 1 x 280ml (64 x 113mm)
 * Insert, 2 x 50ml (29 x 114mm)
 * Delivery BM sited
 * NH1/070 Custom underneath trolley delivery expected 31/08/08

Fri 1st August

 * GE Healthcare XK16/20 column x1
 * GE Healthcare 16/20 tube x1
 * NEB T4 DNA ligase x1
 * NEB Vent Polymerase x1

Weds 30th July

 * Fisher DTT x2
 * Fisher Cuvette Uvette 80 disposable single sealed x1 pack

Mon 28th July

 * ELGA service contracts and 6 month supplies
 * BioRad Short Plates, 5 2packs
 * BioRad Spacer Plates with 0.1mm integrated spacers 1pack

Tues 16th July 2008

 * Fisher NaOH 1M
 * Epp tips 2ul-200ul
 * Epp tips 0.1ul-20ul
 * Epp tips 100ul-1000ul

Mon 14th July 2008

 * HP Printer/copier/scanner and two colour cartridges -

Mon 7th July

 * Fisher LAB-850-112N Biohazard sign x4
 * Fisher LAB-850-113L Biohazard rigid sign x1
 * Fisher LAB-830-176B Now Wash hands sign x2
 * Fisher LAB-835-034X Hazardous Area sign x2
 * Fisher LAB-850-086L Flammable gas sign x1
 * ARCO Ergoclean Mop box with lid

Thurs 3rd July

 * NEB T4 DNA Ligase x2
 * NEB Vent DNA Polymerase

Tues 1st July

 * Fisher AKL-300-030M Foil 450mm x 75M  x2
 * Fisher AUY-200-030K 9.5L sterilising pan x1
 * Fisher AUY-220-070H 3.1L sterilising pan x1
 * Fisher BPE-1760-25 Ampicillin sodium salt 25g x2
 * Fisher CRY-580-010X Cooler labtop for 12 tubes x1
 * Fisher FB55924 Cuvette UV single use 1.6ml 1 box
 * Fisher FB56209 Gel loading tips 1-200ul 1 box
 * Fisher FB71251 Cryobox with cardboard lid white 32mmx32mm 2packs
 * Fisher PDT-100-010M Incubation tray for petri dish
 * Fisher PMP-890-511T 100ul-1000ul pipettor tips eppendorf 5 boxes
 * ARCO Fliptop bin blue 54L x1
 * ARCO Fliptop bin red 54L x1
 * Ergoclean mop handle 2.5M long x1
 * Ergoclean Wet multifibre mix head x1

26th June

 * Amazon HP Ink Cartridge Tricolour x2
 * Amazon Photosmart C3180 All-in-one Printer

24th June

 * Fisher SAT-670-010M Spillage clean up 3M chemical sorbent sheets 4 boxes
 * Perbio from Thermofisher 23225 Bradford Assay Kit 500rx x1

23rd June

 * Merck 324355 DM 25g
 * Promega R6801 Nde1
 * Promega R7131 Nae1
 * Fisher BPE-2553-100 Kilobase Marker
 * Fisher BPE-LAD4-200 DNA MW Markers 25-500bp
 * Fisher Invitrogen VX11815032 Kanamycin Sulphate
 * Bradford reagent or stuff to make it - have made enquiries. Should be ordered Fri 13th.
 * Welded 2 tier trolley
 * Sterile pipettes 5ml
 * Sterile pipettes 10ml
 * Sterile pipettes 25ml
 * Sterile pipettes 50ml
 * Rhamnose (sugar - Fisher 17408-0250 for 25 g)
 * BioRad- Bio-Safe Coomassie Stain, 5L x1 A box with a tap on for easy dispensing


 * BioRad- Precision Plus Kaleidoscope Standards 500ul (50 applications at 10ul per well) x2 This is a rainbow marker which I used at Reading Uni and was brilliant because there is never a doubt about the size of each marker band. I'v ordered 2 so we can use one and when that is used up we re-order so we never run out. To be stored at -20 when not in use. Store at room temp or in cold room when in use.


 * Syringe filter sterile 0.2um 25mm
 * Syringe filter sterile 0.2um, 4mm
 * Vivaspin protien concentrators 0.1-0.5ml
 * Merck Dodecyl Maltoside 5g, overnight shipment requested
 * -80 freezer storage-fisher

Weds 4th June

 * Ep tips box 0.1-20ul

===MAY

Thurs 29th May

 * Fisher Revco racking x3

Fri 23rd May
BioRad Biosafe Coomassie Stain 5L

Tues 20th May

 * Novagen Rosetta2 pLyS (cat# 71401)
 * Promega KRX Competent cells (20 x 50 uL - cat# L3002)
 * Fisher 15ml tubes
 * Fisher 50ml tubes
 * Lab coats XXL light blue x3
 * 0.1ul-20ul tips in box x3
 * 100ul-1000ul tips in box x4
 * 5ml tips in box x2
 * 2ul-200ul tips in box x4

13 May

 * Ammonium Sulphate 2x500g
 * IPTG 2x10g
 * 4L flask x8
 * Face Shield sign x2
 * Face Shield x2
 * XL lab coat x6
 * XXL lab coat x3


 * HiTrap Desalting column 5 × 5 ml 17-1408-01 	£112.00 GE life sciences
 * dodecyl maltoside 5g

arrived (ordered 1st May 2008)

 * BPE1422-2	Yeast Extract 2kg x1
 * BPE-310-500	HEPES Crystals 500g x1
 * BPE328-1	Sodium Hydrogen Carbonate x1
 * CRY-190-010Q	Cryobox 10pk x2
 * FB50323	Weighing boat 5ml 1000pk x1
 * FB50325	Weighing boat 30ml 1000pk x1
 * FB50327	Weighing boat 100ml 1000pk x1
 * FB50377	Weighing boat 250ml 1000pk x1
 * CRY-595-030P	Centrifuge tubes 35ml 10pk x2

arrived 22nd May 2008

 * 50 ml Falcon Tubes
 * 15 ml Falcon Tubes
 * Petri Dish Rack

APRIL
ordered 11th April 2008 Fisher order 7515525 1104081
 * 10pk 500ml beakers
 * 2 x 5pk 1L beakers
 * 10pk 250ml beakers ordered but out of stock til May!
 * 10pk 100ml glass beaker
 * 10pk 250ml glass beaker
 * 10pk 600ml glass beaker
 * 3x 1L glass beaker
 * 2 x 1000pk PCR tubes 0.2ml
 * 1.5ml tube float rack 4pk
 * 0.5ml tube float rack 4pk
 * 25g low melting agarose
 * 5 thermometers -10 to 110 degrees
 * 0.5ul-10ul tips 10 x 1000pk ordered but out of stock til May!

ordered 7th April 2008 Screw Fix
 * 3 shelf units for cold room

ordered 2nd April 2008
 * Fisher Incubator Shelf & 2 Supports x2 ING-205-502L

11th April 2008

 * ELGA cartridges LC148, LC163, LC181, CT all x2

10th April 2008

 * Steps/ladders from screw fix

9th April 2008

 * Fisher 100 mg anhydrotetracycline (not Louise's GPC)

8th April 2008
Screw Fix
 * Trolley for lab move and/or fermentor
 * Spirit level for lab
 * 6 containers for transporting bottles across lab
 * Mobile step for office

3rd April 2008

 * Fisher 5L Beakers x5 FB33119 £11.74 each
 * Fisher Magnetic Retriever x1 FB55669
 * Fisher Stirrer fleas 10pk x1 FB55601
 * Fisher Stirrer fleas 10pk x1 FB55591

MARCH

31th March 2008
ordered 26/03/08
 * BPE2543-2 Thrombin, bovine plasma Fisher BioReagents from Fisher Scientific
 * Merck- n-Dodecyl-b-D-maltoside, ULTROL® Grade Cat. No. 324355 5g

28th March 2008
ordered 27/03/08
 * Fisher- Protein Concentrators 2ml 10KMW Millipore
 * Fisher- Containers for agar plates

27th March 2008

 * Ultra violet DNA gel lamp and spares
 * DNA marker (e.g. Fisher BPE2553-100)
 * GE Healthcare XK 16/20 column 18-8773-01
 * GE Healthcare HiPrep 16/10 DEAE-FF 17-5090-01

20th March 2008

 * ordered 07/03/2008
 * 3kg balance - Fisher
 * 0.5ul-10ul tips 1000pk - Fisher

13th March
These inc VAT £131.55 Ref.7482996 Gail x2234
 * ordered 12/03/2008
 * T4 Polynucleotide kinase - recombinant from E.Coli Fisher BioReagents 100 U BPE3212-1 £23.50 Fisher Scientific
 * PCR tube Fisherbrand thin walled domed cap polypropylene 0.2mL natural pack of 1000 FB68720 £64.60 Fisher Scientific x2
 * ordered 11/03/2008
 * Fisher GHL-415-010D gas burner lighter x2
 * Fisher FB67020 pH Electrode x1
 * Fisher FB70260 Timer x6
 * Fisher BMD-110-021W Batteries x6
 * Fisher LAB-550-010W white tape x1
 * Fisher LAB-551-010Q Orange tape x1
 * Fisher LAB-553-010E Red tape x1
 * Fisher LAB-555-010E Yellow tape x1
 * Fisher LAB-557-010D Green tape x1
 * Fisher LAB-559-010X Blue tape x1
 * Fisher LAC-730-010K Black pen 10pk x1
 * Fisher LAC-730-020H Blue pen 10pk x1
 * Fisher LAB-340-030Q Round labels 1000pk x1
 * Fisher HW1-LB-S small lab coat x9
 * Fisher HW1-LB-M Medium lab coat x9
 * Fisher FB55301 5pk Clamp set
 * Fisher FB68403 Base for clamp x2
 * Fisher FB55305 Bosshead Clamping screws 5pk x1
 * Fisher FB34531 1-200ul tips 1000pk x10
 * Fisher FB34511 0.5ul-10ul tips 1000pk x10
 * Fisher FB34611 200-1000ul tips 1000pk x10
 * Fisher FB70173 cleaning detergent
 * Total of order inc VAT £1367.22

12th March

 * Sigma low range molecular weight markers Sigma 6.5 - 66 kDa M3913-10vl x1
 * Sigma Mid range molecular weight markers Sigma 6.5 - 200 kDa S8445-1vl x4
 * Sigma high range molecular weight markers Sigma 36 - 20 kDa S8320-10vl x1
 * Sample buffer solution Sigma S3401-10vl x2
 * LDao (n,n-Dimethyldodecylamine n-oxide) Sigma D9775-SG x1
 * Spatulas 0.09inch tip Sigma Z243213-2EA £11.80 x10

11 March

 * ordered 10/03/2008
 * Promega Wizard Gel and PCR Clean up Kit 250rx A9282 x1
 * GEHealthcare - PEEK tubing 1/16", i.d. 0.75 mm 2 M 18-1112-53 1  £50.50   £50.50
 * Superloop, 1/16" fittings (ÄKTAdesign) 10 ml 1 EA 18-1113-81 1  £360.00   £360.00 Sub Total: £410.50
 * n-dodecyl beta-D-maltose D4641-SG - Merck
 * cAMP-Merck
 * BL21 (DE3) competent cells -Merck

10 march

 * 2 x 100g electrophoresis agarose - Fisher
 * 1Kg Ammonium Acetate - Fisher
 * 1ul-200ul tips 1000pk - Fisher
 * 250ul-1000ul tips 1000pk - Fisher
 * 3.5 KDa cut off dialysis tubing
 * PCR thermocycle control panel
 * Miniplasmid kits
 * Acetic acid
 * DTT
 * glycine
 * sodium bicarbonate
 * EDTA
 * chloramphenicol
 * butane cartridges for bunsen
 * ethidium bromide
 * kanamycin sulphate
 * ampicillin
 * 0.45 u sterile filters

4 march

 * Top 10 cells
 * Arabinose

FEBRUARY

25 Febuary

 * Yeast extract
 * 0.22 um u sterile filters
 * X-gal
 * KCl
 * KH2Phos
 * Na2Hphos
 * Triton X-100
 * biohazard autoclave gloves
 * bunsen burner
 * batteries
 * orange bins
 * SDS

20 February

 * 1000 MWCO Spectrapor dialysis tubing
 * ammonium acetate
 * lysozyme