User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/06/10

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General
From the article: "Robustness and the cycle of phosphorylation and dephosphorylation in a two-component regulatory system"
 * EnvZ is less abundant than OmpR : the steady-state level of OmpR-P is insensitive to variatios in the concentration of EnvZ.
 * OmpR-P is insensitive to variations in the concentration of OmpR when the OmpR concentration is sufficiently high.
 * At low concentrations of OmpR-P ompF is activated.
 * In EnvZ the autokinase, phosphotransfer and phophatase reactions overlap and interact.


 * The numbers of EnvZ and OmpR molecules per cell have boon determined to be 100 and 3500, ratio EnvZ-to-OmpR ratio of 3%.
 * YFP fluorescence remained relatively constant over the full range of [EnvZ]T Undetermined factors regulate ompC expression.
 * Possible that very high levels of unphosphorylated OmpR could activate ompC and repress ompF
 * The change on ompC/ompF transcription as a function of osmolarity is quite gradual.
 * Incorporation of OmpR-DNA binding
 * EnvZ dimerization, binding of OmpR to EnvZ, conformational changes of OmpR