IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-6

Plates and colony PCR
We got decent growth on both plates (50+ on one, only 8 on the other); doing a colony PCR for 15 colonies,8 and 7 for J360012+J360010 and J360011+J360010 respectively.


 * 95C for 15'00
 * Do 30 times:
 * 95C for 0'30
 * 55C for 0'30
 * 72C for 3'00
 * 72C for 10'00
 * 4C forever

8uL PCR supermix 1uL 2uM VF2 1uL 2uM VR

2 Plates ready to be taken out (restreaks) @ 1200 tomorrow.