IGEM:MIT/2007/Notebook/2007-8-13

Agenda

 * 1) Check sequencing results of constitutive promoters + CPX
 * 2) Dilute last night's LCs (F2620.B0034.CPX.B0014)
 * 3) Miniprep half of last night's LCs and send in for sequencing
 * 4) Live cell fluorescence immunoassay (or western blot) with f2620.b0034.cpx.b0014 transformed cells in M9 media

SUMMARY

 * 1) Ran polystyrene wash assays on various concentrations and wash compositions of CPX-inserted cells
 * 2) Concluded that overexpression of CPX in more than 10e-5 M AHL is toxic to cell, and most efficient induction happens between 10e-6 and 10e-7 M AHL
 * 3) Grew up individual parts on Mer-GFP system
 * 4) Currently working on sending Biobrick I13500 to sequencing

FUTURE PLANS

 * 1) Run gel digestion and purify I13500
 * 2) Run PCR and purify MerR/MerPT
 * 3) Either do two standard assemblies or one 3A assembly on a TK Precut Plasmid
 * 4) Send in for sequencing and do mercury assay test