User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/27

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June 27th, 2010
I made two ligations and I also ligated two controls:

luxAB --- ligated --- plasmid 17

luxAB --- ligated --- plasmid J61002 carrying ampicillin resistence (with constitutive promoter J23106)

Controls

Just plasmid 17

Just plasmid J61002

I followed the next protocol for ligation:

5μL luxAB (insert)

2μL vector

2μL Ligase Buffer 10x

1μL T4 ligase

10μL H2O

And I incubated during 19h to 16°C


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