User:Jessica Karen Wong/Design

Constructing an output measurement kit derived from  T9002 consisting of
 * an inducible promoter  F2620 which produces POPs in the presence of AHL
 * a Biobrick site
 * The ribosome binding site  B0032
 * The Green Fluorescent Protein  E0040
 * The terminator  B0015

Assisting in the construction of
 * Promoter tester derived from  E0240
 * RBS tester derived from  I2055

Building devices containing RFP  E1011 and LacZ  I0054</BBpart>
 * Similar to  E0240</BBpart> containing  B0032</BBpart> and  J04650</BBpart>
 * Similar to  I2055</BBpart> containing  R0040</BBpart> and  J04650</BBpart>

Progress

T90002
F2620________E X P1010 S P________E0040

Should be on both 3K3 and 1AK3

E0240

 * Version 1 (E/S)
 * ___E CCDB S___RBS GFP T
 * Cut promoter E/S and insert
 * Can take part back out if necessary


 * Version 2
 * ___E CCDB X___E0240
 * Cut Promoter E/S to insert
 * Forms mixed site so can't remove, real conditions


 * Both versions should be on both 3K3 and 1AK3
 * I2057 is the exact same but w/ RFP (both versions)

I2055

 * Versions 1 (E/X)
 * __R0040___E CCDB X____GFP T
 * To insert RBS cut E/S, forms a mixed Spe/Xba site
 * Has too big spacing (full BB prefix) b/t promoter and RBS
 * Easier for testers to use


 * Version 2 (S/X)
 * __R0040___S CCDB X____GFP T
 * Will get 2 spe/xba mixed sites, can't get RBS out
 * Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation
 * Real condition spacing


 * Both versions should be on both 3K3 and 1AK3
 * Theoretically I2056 should be the same w/ RFP