User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/27

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] AU CHEM-570 Lab Prep
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Objective

 * Confirm that PCR worked and product is consistent with expectations. Also confirm that miniprepped plasmid is consistent with expectations.

Bench work

 * 1) Analytical digest of pCMV-SPORT6+BSA] with  XmnI
 * 2) *Daniel Catt did this
 * 3) *8.9μL 158μg/mL pCMV-SPORT6+BSA + 1μL 10X  NEB4 buffer + 0.1μL 100X  BSA + 1μL 20kU/mL  XmnI
 * 4) *&rarr;1h @ 37°C
 * 5) *&rarr;4°C
 * 6) Analytical minigel
 * 7) *1.2% Agarose gel (~20 mL with small-tooth comb)
 * 8) *Mix samples with 6X DNA loading buffer and load:
 * 9) 10μL 1KB ladder
 * 10) 10μL XmnI-digested plasmid from step 1
 * 11) 5μL PCR product from yesterday, step 3

Results and Conclusions
Results are consistent with expectations.


 * }