IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/21

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

June 21, 2010

 * Met with Synthetic Biology Research Group
 * Miniprepped RFG/CFG/YFP using 50 and 30 ul elution volumes
 * Quantified DNA from minipreps (compared 50 ul and 30 ul water additions to the last step)- (solution: team member/fluorescent protein used/ul of water added at the last step)

Solution	Value (ng/ul)	 Solution	  Value (ng/ul) JE-YFP-50	  7.7	         DN-YFP-50	    22.1 JE-YFP-30	  2.3	         SB-YFP-50	    5.8 AC-CFP-50	  8.7	         SB-YFP-30	    5.1 DC-CFP-50	  3.2	         DN-RFP-50	    19.5 DC-CFP-30	  1.0	         DN-RFP-30	    18.6 BT-CFP-50	  16.6	         JC-RFP-50	    19.4 BT-CFP-30	  8.2	         JC-RFP-30	    8.6


 * Prepared Terrific broth
 * Autoclaved assorted glassware/plastic ware
 * Plated 100 ul of TOP10 (for testing antibiotic media as a control) and new RFG/CFG/YFG
 * Made overnight cultures of CFG/RFG/YFG/TOP10 in Terrfic broth
 * Looked up metal toxicity levels for fish (mercury, copper, and arsenic)
 * Sent emails to other subteams about regional meeting and design changes


 * }