User:Karmella Haynes/Notebook/Polycomb project/2010/04/19

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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04/19/10

 * &#x2713; KAH131-9: Split 96-well cultures to 2 new plates, dox-induce one plate
 * &#x2713; KAH131-9: Pick colonies for new 96-well set
 * &#x2713; Transfection prep: U2OS plain, 12-well plate (1x105/ well); for testing H3me reporters (+/- KAH60)

KAH131-9 colonies > 4/20/10: No RFP+ in any dox+ wells > Added 1μg/mL dox to remaining colony plate to see if there is any expression at all > 4/21/10: saw no expression on entire plate. Discard KAH131-9 cultures and try again later.


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