IGEM:IMPERIAL/2007/Projects/Cell by date/Implementation/List of Required Parts

pTet GFP



 * Registry part: BBa_I13522
 * Comments: Untagged GFP behind a constitutive promoter.
 * DNA Available in the registry



pT7 GFP



 * Registry part: BBa_J34814
 * T7 promoter sequence: gaatttaatacgactcactatagggaga
 * Comments: T7 Promoter to be used qith t7 polymerase BBa_J34811
 * DNA still in planning
 * pT7 sequence from Ambion:

pBad GFP



 * Registry part: BBa_J5528
 * Comments: Similar to part J5527 where mRFP is replaced with GFP
 * DNA Available in the registry
 * Allows tight control in E. coli

Generic Components
BBa_B0034
 * RBS (Elowitz)

Short description: This is the most efficient RBS in the registry, and sets the 1.0 efficiency standard. It is based on the Elowitz repressilator.

BBa_B0015
 * Double terminator (B0010-B0012)

Short description: Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable. -forward_efficiency: 0.984 -reverse_efficiency: 0.295

Other Materials

 * DsRed-Express


 * T7 RNApol

BBa_J34811

Short description: To be used with specal tRNA loading glutamate instead of stop codon (Part BBa_J34812).

BBA_J34812
 * tRNA loading Glutamate on stop codon TTT