Shreffler:Notebook/R03 Baso/2010/07/29

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Basophil Project
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Basophil Sensitization (8) - 7/30/10

 * Patient was already sensitized to rDerp2, as with (7)
 * Sub-optimal/heterogeneous antibody staining, possibly due to high cell density (clumps were present in almost every wash step, as well as in final volume run in flow)
 * Labeling discrepancy - rows 2-4 (H:P 1:99, 50:50, 99:1) were labelled in reverse order on FACSDiva, but I'm 90% sure that H:P 1:99, 50:50, 99:1 was correct order


 * 1) [[Media:BasoSens08_20100730.pdf]]
 * 2) [[Media:BasoSens08_20100730-Table.txt]]
 * 3) [[Media:BasoSens08_20100730-Table.pdf]]


 * }