User:Karmella Haynes/Notebook/miR Trigger/2011/02/06

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01/06/11

 * &#x2713; Transfections: miR sensors into U2OS plain and JDS33-47

Lipo transfection > 2 Plates
 * 1) U2OS plain
 * 2) JDS33-47

> Add Lipo to 250 μL Opti-MEM --> R.T/ 5 min. > Add DNA to 250 μL Opti-MEM > Add DNA mix to Lipo mix --> R.T./ 20 min. > Plate ~2.5 x 105 cells in each well; use 3.5 mL medium > Add 500 μL complexes to each well (4 ml med. each); Grow cells at 37&deg;C > Change to plain medium and grow for 3 days


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