IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week8/Widgetry

=Goals for this week=


 * 1) Run co-culture test with DH5α, DH5α/pUC19, and Shewie
 * 2) Move chambers to the "dark" to prevent a reaction from happening with light and oxygen
 * 3) Test light-repressible system on plates

=Monday: August 11, 2008=


 * cleaned chambers
 * moved setup to a cabinet to have system in the "dark"

DH5α, DH5α/pUC19, and Shewie co-culture experiment setup

 * Chamber 1: DH5α + wt Shewie + Lactose
 * Chamber 2: DH5α + wt Shewie + Lactate           (pos. control)
 * Chamber 3: DH5α + wt Shewie                     (neg. control)
 * Chamber 4: DH5α/pUC19 + wt Shewie + Lactose
 * Chamber 5: DH5α/pUC19 + wt Shewie + Lactate     (pos. control)
 * Chamber 6: DH5α/pUC19 + wt Shewie               (neg. control)
 * Chamber 7: wt Shewie + Lactose                  (neg. control)
 * Chamber 8: DH5α + Lactose                       (neg. control)
 * Chamber 9: DH5α/pUC19 + Lactose                 (neg. control)

=Tuesday: August 12, 2008=

=Wednesday: August 13, 2008=

RU1012 Light Repressible system experiment

 * Streaked plates.
 * All Cm/Amp plates


 * Dark
 * Plate 1: RU1012 w/ plasmids
 * Plate 2: RU1012
 * Plate 3: Negative control (No bugs)
 * Light
 * Plate 1: RU1012 w/ plasmids
 * Plate 2: RU1012
 * Plate 3: Negative control (No bugs)

=Thursday: August 14, 2008=

RU1012 Light Repressible system experiment

 * Spread plates to try to create lawn.
 * All Cm/Amp plates


 * Dark
 * Plate 1: RU1012 w/ plasmids
 * Plate 2: RU1012
 * Plate 3: Negative control (No bugs)
 * Light
 * Plate 1: RU1012 w/ plasmids
 * Plate 2: RU1012
 * Plate 3: Negative control (No bugs)

=Friday: August 15, 2008=

RU1012 Light Repressible system experiment

 * Spread plates to try to create lawn.
 * All Cm/Amp plates


 * Dark
 * Plate 1: RU1012 w/ plasmids
 * Plate 2: RU1012
 * Plate 3: Negative control (No bugs)
 * Light
 * Plate 1: RU1012 w/ plasmids
 * Plate 2: RU1012
 * Plate 3: Negative control (No bugs)