User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/01/25

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Mount ant-sa and anti-bam samples
 * Stain and mount totpro sample
 * Stain with ToPro3 1:1000 in 2% NGS
 * 30 mins at RT
 * Wash 4 x 5 minutes in PBST
 * Mount in Vectashield


 * PCR of recombinants(?) with flpase primers
 * Run normal PCR with 94C melting phase, 52C annealing phase, and 45' 72C extension phase
 * 2x normal magnesium
 * 4 reactions: -, +, exp1, and exp 9.
 * Expecting 700bp band


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