User:Kalkao/notebook/Streaking Plates


 * 1) Obtain cell stocks from the -80*C storage and place in a cold block
 * 2) Obtain T-broth agar plates from the 4*C fridge
 * 3) Use a sterile loop to streak cells onto the plates
 * 4) *Use fireboy to flame sterile loop
 * 5) *Dip the loop approximately 1/4 inch into the BL21 stock
 * 6) *Spread three lines of stock culture onto one side of the agar plate
 * 7) *Spread three lines of the culture on the plate across the top of the agar with the sterile loop
 * 8) *Rotate the plate 90* and spread three lines of culture across the top of the agar again from the existing streaks
 * 9) *Rotate the plate 90* and make three final streaks across the top of the agar
 * 10) *Be sure to flame the sterile loop between each set of three streaks
 * 11) *After flaming the sterile loop, cool the loop by touching it to the agar before you streak cells
 * 12) *Remember to use good sterile technique so that the plates do not become contaminated
 * 13) *Repeat this step for each stock on a new T-broth agar plate
 * 14) When streaking is finished, put the plates in the 30*C incubation room to allow the colonies to form slowly
 * 15) *The colonies should be ready in approximately 15 hours