User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/07

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Spermatogenesis

 * Continue Dissections


 * Wash 3 x 15 minutes in 1x PBS-T
 * Incubate with secondary antibody in 2% NGS (1:500, anti-guinea pig, 488nm)
 * 2hr at RT
 * Wash 3 x 15 minutes in 1x PBST
 * Incubate at 4C over weekend. DNA stain/mount on Monday/Tuesday


 * }