Shreffler:Notebook/Staff Meetings/2010/08/24

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] ShreffLab Staff Meetings
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Agenda Items

 * EoE
 * EDN ELISAs
 * Sarita has ordered a kit to try? and will initially evaluate with stds from company? -- will ship on 9/3
 * Conference call with Mirna on Friday to discuss possible collaboration and availability of samples for validation
 * has some samples to send us
 * Component proposal -- WS still needs to work on
 * Basophil
 * Review data from last week
 * responder, good IL-3 enhancement; detectable IL-13 with dose response at 6 hours and detectable at 18 hours, though maybe predominant IL-3 effect
 * Review ALK data
 * responder -- good responses at 1:1; expected results -- when will we get more mAb?
 * Histamine data
 * we have not seen more data
 * send sups from this week
 * Next experiments
 * Repeat isolated basophil experiments this week
 * Allergen Adjuvant
 * 2D done by Galina
 * confirms pI ~10
 * Hazmik has gels and will run this week
 * needs plant protein in fasta
 * Foreskin DC flow data
 * unable to detect aldefluor -- surface marker staining is weak prob due to staining in large vol with substrate buffer
 * discussed alternatives, will first try staining per routine following aldefluor incubation, then may try sorting prior to culture or sorting after for qPCR


 * PM Baso
 * no samples last week
 * JAX
 * quote for peptide arrays
 * WS has to do
 * train Stephanie for project role
 * Stephanie almost self-sufficient now
 * Carboplatin
 * possible sample Wed
 * U19
 * Set up PBMC last week to milk allergen, sort on Fri
 * set up another this week
 * contact Josh about buffer

Other Discussion

 * Hood certification/ CO2 tanks
 * will take over cost of that room

Things To Do

 * 1) Write up component proposal (Marcella and Wayne)
 * 2) obtain quote for peptide arrays
 * 3) send Bert U19 slides