User:Fermenter User/Notebook/MACG G3

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs  Pink: MeOH Induction hrs

MACG G3

Day -3 (Jul 24 Fri):
             7/24/2009 (00:00)     Prepare and autoclave additives: Prepare media (1-L/fermenter) with antifoam

Day 0 (OCt 13 Tue):
 MeOH Induction Day 1 ==

             10/13/2009 (00:00)    Take a colony from plate in 600 ml of BMGY(4 500ml flasks) Allow it to gorw at 30°C.

             10/13/2009 (11:00)     Bring PBS bf(1-L/bioreactor) to fermenter room           10/13/2009 (11:00)     Calibratiron of pH sensor No Antifoam added (it is already in media prepared by Gunna) 10/13/2009 (13:30)    Autoclave start 7/27/2009 (17:28)    Start Fermenter #1 software 7/27/2009 (17:00-18:00)Media transfer 7/27/2009 (18:00)    Set rpm at 800 7/27/2009 (18:00)    Start temp control

     7/27/2009 (18:30)    Fermentation 00:00 Inoculate cells into fermenters

Day 1 (Jun 28 Tue):
 MeOH Induction Day 2 ==

Emergency building closure

Day 2 (Jun 29 Wed):
 MeOH Induction Day 3 ==      7/29/2009 (11:00)    Fermentation 40:30 MeOH calibration (5 ml 100% MeOH injection to 1-L media) 7/29/2009 (when?)   Fermentation 00:00 F1 (OD: ?? ) F2 (OD: ??  ) F3 (OD: ??  ) 7/29/2009 (13:00)   Fermentation 44:30 Connect MeOH pump (F1)(F2) 7/29/2009 (13:15)   Fermentation 44:45 F1: Add Sorbitol F3: Add Casamino acid + Sorbitol 7/29/2009 (15:30)   Fermentation 47:00 Connect MeOH pump (F3)

Day 3 (Jun 30 Thu):
 MeOH Induction Day 4 == <SungHye/Gunna>     7/30/2009 (13:00)    Fermentation 68:30 F1 (OD: ?? ) F2 (OD: ??  ) F3 (OD: ??  ) F1 (MeOH: ml) F2 (MeOH: ml) F3 (MeOH: ml)

Day 4 (Jun 31 Fri):
<font color=#FF00FF> MeOH Induction Day 4 == <SungHye/Gunna>     7/31/2009 (13:00)    Fermentation 92:30 F1 (OD: ?? ) F2 (OD: ??  ) F3 (OD: ??  ) Harvest


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