IGEM:MIT/2006/Notebook/2008-1-22

=Mini Time Course=

Time Zero for J45200- 7:20

Time Zero for J45250- 7:40

Pulse for J45200- 11:20, 12:20, 1:00

Pulse for J45250- 11:40, 12:40, 1:20

GC extracts for J45200- 2:20, 3:20, 4:00

GC extracts for J45250- 2:40, 3:40, 4:20

GC extract of J45200-PC (OD600=2.02), J45900 (OD600=2.26), and TOP10+PC (OD600=2.82)- Start at 11:40

OD600s

Before IA Addition

J45200

4:00- 0.04

5:00- 0.14

5:40- 0.32

J45250

4:00- 0.04

5:00- 0.24

5:40- 0.46

After IA Addition

J45200

7:00- 0.12

8:00- 0.70

8:40- 1.12

J45250

7:00- 0.20

8:00- 1.10

8:40- 1.28

=Set up cultures=

1. 60 mL J45700- 250-mL baffled

2. 60 mL J45120- 250-mL baffled

3. 25 mL J45120+PC- 125-mL

4. 25 mL TOP10+PC- 125-mL

5. 115 mL J45200- 250-mL baffled

6. 115 mL J45900- 25-mL baffled