IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/14/Min Lin

10:00 Pour 1% agarose gel. Pre-mix the plasmid digestion product with 4ul geneFinder. Run the gel with DL2000 plus marker from Transgene. 60V to enter the gel, and then with 120V The insert in lost.

10:50 Pick 1-12M colony and 1-12O colony into 5ml Amp+ LB, shake in the incubator.

11:00 Sterilize LB.

14:30 Pour LB plates(Cm+)