User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/06/01

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June 1st, 2010
1. PCR with RTTH
 * PCR was done for Blue Promoter (2 reactions) and OGR as a control for primers. Reactives needed are as follows for one reaction with 2 ul of DNA each one:
 * Reaction1
 * HPLC	12
 * Buffer 3.3x	6
 * Mg(Ac)2	3
 * dNTP’s	2
 * Primer FWD	2.5
 * Primer REV	2.5
 * DNA	2
 * Total	30


 * Reaction 2	
 * HPLC	10.5
 * Buffer 3.3x	9
 * RTTH	0.5
 * Total	20

2. PCR product was purified using the High Pure PCR Product Purification Kit and an agarose gel 3% was run to verify PCR product.



Lanes: 1,5) Ladder; 2) BP1; 3) BP2; 4) OGR


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