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Madhvi Venkatesh

Flagellar Display
Source: ???? Start here: PMID: 16085812 and here: http://tools.invitrogen.com/content/sfs/manuals/flitrx_man.pdf and also consider this one: PMID: 19060885

Your goal is to make a new flagellar display system, possibly a circularly permuted flagellar protein, or a secreted adapter fusion protein. We want a system that is N-terminal or C-terminal (not in a loop). The most likely candidate is FliD, but also consider FliC and other E. coli K12 flagellar proteins, etpA and it's Yersinia pseudotuberculosis homolog CP000720.

You may also need to use a fli gene knockout. Once you know what you are going to make, we'll obtain the clone from the Keio collection.

Questions you need to ask when reading:
 * Is there a reference showing your target protein can be knocked out and re-complemented resulting in effective chemotaxis?
 * Is there structural information sufficient to guide the construction of a circularly-permuted variant?

In the end, you'll be making 1 or more Displayer parts. You'd probably want to try both N and C terminal display types. Follow these instructions about where to put your part based on the type:

N-Terminal Displayer Info C-Terminal Displayer Info