IGEM:Harvard/2006/vlau/Notebook/2006-7-26

PEG precipitation
1. Rxn Mixtures 1: 20 c4.0Fb + 20  20%PEG + 160  d    2: 20  c4.0Fb + 30  20%PEG + 150  d    3: 20  c4.0Fb + 40  20%PEG + 140  d 2. Protocol - incubated on ice for 15 min. - spun @ 16,000 rpm for 10 min.   - supernatant removed into separate tubes and pellet resuspended in 1x volume folding buffer to supernatant