IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/14

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Tuesday 14th September

 * Carried out plasmid mini preps of all the successful transformations using the overnight cultures from yesterday.[]
 * Set up an overnight digestions of the promoters using the following method:

Plasmid - 10μL Pst1 - 2μL EcoR1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL
 * Promoter digest:


 * Set up an overnight digestion of part BBa_J04450, using the following method:

Plasmid - 10μL Pst1 - 2μL EcoR1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL
 * BBa_J04450 digest:


 * Part BBa_J04450 is a RFP contained in a pSB1C3 plasmid and is also Cm resistant. This digestion is therefore to remove the RFP so that this plasmid can be used for submission of our promoter-GFP biobricks to iGEM.


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