IGEM:UC Berkeley/2006/Analitic digestion


 * 1) Use Ape to determine which enzymes to cut product with. Cuts should yield bands that are easily distinguishable on a gel.
 * 2) To digest make a cocktail of NEB2, Enzyme, H2O, and DNA product for a total volume of 10uL.
 * 3) *If DNA product is high copy then use only 2uL of product 1uL of NEB2 and adjust the H2O and enzyme accordingly to make 10uL total:
 * 4) ***2 uL miniprep/DNA product
 * 5) ***1 uL NEB2
 * 6) ***.25 uL enzyme 1
 * 7) ***.25 uL enzyme 2
 * 8) ***1 uL BSA
 * 9) ***5.5 uL water
 * 10) *If DNA product is low copy then use 4uL of product and 1uL of NEB2 adjusting the H2O and enzyme accordingly:
 * 11) ***4 uL miniprep/DNA product
 * 12) ***1 uL NEB2
 * 13) ***.25 uL enzyme 1
 * 14) ***.25 uL enzyme 2
 * 15) ***4.5 uL water
 * 16) Incubate for ~30min. and then run on gel.