User:Tk/Notebook/MF/2009/03/21

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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RNAse H genes; Genomic DNA prep for Illumina sequencing
Kochiwa07 reports on the variety of RNAse H genes in bacterial species. He finds three major categories, RNAseH-I, RNAseH-II, and RNAse-III. MF has RNAseH-I and RNAse-II, but the -I has additional domain dsRHbd (double stranded RNA helix binding domain). The genes in MF for these two are:


 * -I (with dsRHbd) is Mfl475, and is apparently essential
 * -II is Mfl534, and has been knocked out several times.

Made new 1161 plates - no antibiotic

Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing.

Prepare seed cultures of L1, GF1, PP1, W12, M. entomophilum


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