Yu:PNK label

5' end labeling of Oligonucleotides
Adhere to all safety procedures involved with handling radioisotopes!!!

Reaction Mix
Note: Try to keep the dATP/Oligo ratio at ≥ 5:1
 * 1) Mix the first three reagents together, then add γ-dATP, and then finally add the PNK
 * 2) Note: Use filter tips when working with radioisotopes to prevent contaminating your pipetman.
 * 3) Incubate the mixture at RT for 30 min or more, behind proper shielding.
 * 4) Add 80 µL of TE¬8 to the Kinase reaction mixture.
 * 5) Purify the reaction mixture (100 µL) using the mini Quick spin Oligo Columns (Roche), stored in the 4˚C Fridge.
 * 6) Note: You can prep the column ~5min before the Kinase reaction is complete
 * 7) After purification check both the column and the flow-through for radioactivity, both should be very hot.
 * 8) Sample is ready for hybridization.

'''To facilitate the kinase reaction the oligonucleotide should have a 5'-OH. IDT's oligonucleotides already exist like this and do''' not need to be dephosphorylated first.