User:Vijay/Protocol/microarray-rna

Materials

 * RNA Protect (Bacteria) - Qiagen
 * Trizol - Invitrogen
 * Fast Blue - MD (Qbiogene)
 * Chloroform
 * DEPC water
 * 75% ethanol

Procedure

 * Cell Harvest
 * 1) Collect 2ml culture and add 4mml of RNA protect (Qiagen) in 15 ml Falcon tube.
 * 2) Vortex for 5sec, incubate room temperature for 5 min.
 * 3) Centrifuge for 10min at 7300rpm.
 * 4) Dab on paper towel (up side down).
 * 5) Freeze.
 * RNA Isolation
 * 1) Resuspend the pellet in 1ml Trizol (pipette up and down to resuspend) and transfer to FastB tube.

Procedure

 * 1) SDS-PAGE and TRANSFER done as per our lab protocol

1M KH2P04
(No autoclave, no filter, just make it and use it.... in 100ml glass bottle) 13.6gms in 100ml DEPC water