Kubke Lab:/Notebook/Cranial nerve development/2011/01/17

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Cranial Nerve Development
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=General Entries= =Personal Entries=
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Malisha

 * 10.00 AM: Meeting with Fabiana and Reuben
 * 11.00 AM: Nissl stained sections cut buy Fabiana.
 * 12.30 PM: Left for the day.

Reuben

 * 10:00am Group Meeting
 * 10:45am Arranged access to Histology lab
 * 11:00am Began gelatin subbing of slides.
 * During the initial cleaning of the slides there was not enough ventilation for the slides to dry properly and as a result the slides were streaky and murky. I cleaned them in acid alcohol and acetone a second time and this time placed the slides on the heating tray in the fume hood room. This dried them quick enough to remove unwanted streaks in the glass.


 * The bottom of the beaker I was heating to dissolve the gelatin must have got too hot as some of the gelatin denatured and stuck to the bottom of the beaker. I chose to start again making a new gelatin solution. This time I had a paper towel between the heating surface and the beaker, used a lower temperature and added the gelatin slowly to prevent clumping of the gelatin powder. This allowed the gelatin to dissolve as required. --Reuben Cutfield 20:29, 16 January 2011 (EST)


 * 2:30pm Created OpenWetWare page Kubke_Lab:Protocols/Gelatin_Subbing and wrote up the protocol I used today in the talk page]. --[[User:Reuben Cutfield|Reuben Cutfield 21:28, 16 January 2011 (EST)

Plan for the week:

Monday: Sub slides for cutting with gelatin.

Tuesday: Perform serial sectioning of embryo.

Wednesday: Nissl stain the sections. and begin analyzing.

Fabiana

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