JL071106-Construction of pSB1A2-J23012

[SpecR] PCR JL1/JL2 on wt. pSPlam  (917bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521   (EcoRI/SpeI, Neb2, Large 2062) Product is pSB1A2-J23012 - JL1 Forward EcoRI oligo to bioBrick SpecR: gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc JL2 Reverse SpeI oligo to bioBrick SpecR: ccgctactagtCAGCAACGATGTTACGCAGC