SBB09 Construct37

Construction of {} basic part

Wobble Oph024-F/Oph025-R       (136 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5  (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10037    {}

Oph024-F       foward oligo for cloning of {} ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg Oph025-R       reverse oligo for cloning of {} ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc

JCA: wrong vector. See revision below:

Construction of {} basic part

Wobble Oph024-F/Oph025-R       (136 bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5  (EcoRI/BamHI, L) Product is pBca9495AK-M10037    {}

Oph024-F       foward oligo for cloning of {} ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg Oph025-R       reverse oligo for cloning of {} ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc