IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/07

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Transformation of B.S. IA771
New transformation with the same protocol than 2 days ago. We will try to add less liquid on plates (to avoid growing colonies in liquid)

Check Vectors
We want to make a final check for ECE147, 149, 150, 153 and 162.

- Double digest + gel


 * Results


 * Lane3 : HyperladderI
 * Lane4 : ECE147
 * Lane5 : ECE149
 * Lane6 : ECE150
 * Lane7 : ECE153
 * Lane8 : ECE162
 * Lane9 : HyperladderI



- ECE 147, ECE150, ECE153 :ok!

- ECE 149 : 3 bands! not the right vector

- ECE162 : ony one band! It has been uncut... maybe the good vector (according to precedent gel), but not sure

Test ECE112 transformation
- for the three different strain (1A1, IA771, IA751), make 12 spots

- take one colony with a loop, and put it on a Cm5 plate and on a Cm5 + Spc100 plate (do that for the 12 spots)

- incubate

New stock
- Put ECE171 in 10mL of LB

- Incubate


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