SIL072006-Construction of pSB1A2-J23016

F plasmid with trbC knockout PCR JL12/JL13 on pKD3  (~1000bp, dPN1, Neb2) Electroporate into pKD46xpOX38 Product is pSB1A2-J23016 - JL12 Forward oligo to knockout trbC-f: atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag JL13 Reverse oligo to knockout trbC-f: tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatat