IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-30

=Lock & Key By Yu Tao= =oriT Knock Out=
 * By Xu Anting

Competent Cell preparation

 * Prepare to make competent cells using strain F/R751/pSC101
 * Sterilize 1M CaCl2, 100mM CaCl2/15% glycerol.
 * Activation of three strains in LB- plate (pSC101 in Tc+ plate). Hold in 37 centigrade for 16 hours.