IGEM:IMPERIAL/2009/Feedback & Debriefs/Feedback 31 7

=Friday 31st of July 2009=

Timer & modeling

 * the other way of inducing lac repressor is to use
 * IPTG is basically on and off, we might have a number of problems
 * leakiness in the lac promoter
 * the timer is not directly linked
 * reduced tunability
 * rethink the timer?
 * medium defined: put the glucose, put the arabinose, the time delay is directly correlated to the amount of glucose you put in
 * how long does it take to make a recombinant protein?
 * time delays are massive compared to this
 * make the model into a transforming diagram, the new way of communicating the module: in engineering we don't use ODEs, they are hidden in transforms
 * what is the Tet repressing well or not?
 * it seems so
 * Investigating another solution for M1-M2
 * use an activator, it would be better
 * the current solution wouldn't work (probably)
 * Glucose uptake and energy and protein creation
 * you can buy it: novagen
 * taking two existing prts and making a new one out of it, that is positive, still use an activator

Module 3, Killing

 * Good test would be using Dam+ and Dam- strains
 * Is the lambda Cl going to interfere with lacI
 * Why is harvard's 08 biobrick so bad?
 * we should say that at the jamboree
 * It has to be a tight promoter

Overview

 * Module 1 could take ~5hours
 * Module 2 could take ~2hours
 * The flow diagrams are good
 * we should produce three nice models that we can then link together
 * We should probably PCR them all out, much faster
 * Using ligation-indipendent cloning can overcome many of the time limitations
 * Dam doesn't need synthesis