Kubke Lab:Research/CND/Records/RC003

=Embryo details=

Species: Gallus gallus domesticus Embryo Name: RC003 Embryo stage:ST21 Staging description:Confirmed by Malisha and Fabiana. Fixation: PFA Cryoprotection:30% Sucrose solution. Material label and storage:Slides kept in slide folder labeled RC003.

=Experiment details=

Objective: Use the embryo to practice sectioning in the cryostat. Investigate the settings which will provide the best quality sections for studying the histology of the tissue. Use the tissue to evaluate the best staining protocol to use to visualize the tissue histology. Procedure: Comments:
 * Embryo staged under a dissection microscope following removal of PFA, replenished with .9% saline solution. See Notebook entry.
 * Embryo was placed in 30% sucrose solution and allowed to sink.
 * Fabiana cut the embryo in the cryostat and mounted the sections onto polylysine slides. See Notebook entry.
 * I performed an H&E stain on the odd numbered slides and a Cresyl Violet stain on the even numbered slides. See Notebook entry.

H&E staining For more information see Kubke Lab:Protocols/Hematoxylin Eosin

Cresyl Violet staining For more informattion see Kubke_Lab:Nissl_Stain_Protocol

=Results=

Cresyl Violet staining gave better results than H&E staining. Based on the results we will discontinue staining with H&E.

=Images= =Summary=