User:Steven J. Koch/Notebook/Kochlab/2009/08/28/SapI Oligo Order

Capping hairpin 2
Steve Koch 12:38, 11 August 2009 (EDT): OK, here's a google doc with my notes, and hopefully the same sequence as in the note below:

Capping hairpin
Here is the hairpin I used in grad school: Unbiotinylated end-cap, HindIII

5’-P-TCGAGGCGACCTAGCGTAAGCTAGGTCGCC Name = P16-Cap-HindIII(incorrect!!!!) L=30 Purification = HPLC Modifications = 5’ phosphate for ligation I think you should:
 * Check with m-fold that it's a hairpin
 * replace the 3 or 4 base sticky end with 3 or 4 based for SapI that you have