User:Shahar Sukenik/Notebook/p53CT expression in minimal media/2010/08/03

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03/08/10: Bacteria growth

 * Started growing at 10:00. Had 2 x 250 mL M9 media (one for 17°C and one for 37°C) and 1 50 mL 2xYT+NPS as control.
 * Inserted 1:100 volume of starter solution, which seemed good.
 * Started checking OD:


 * At 16:00 I took the ctrl flask from 37°C, added 6 μL 840 mM IPTG and inserted to 17°C to induce expression O/N.
 * M9 flasks did not show any growth.