IGEM:IMPERIAL/2007/JournalClub

Hrp System
Alex and Maira Ben and Anthony Cheuk and Dirk Peixuan and Jerry
 * "Enhancer-Binding Proteins HrpR and HrpS Interact To Regulate hrp-Encoded Type III Protein Secretion in Pseudomonas syringae Strains", Steven W. Hutcheson et al, Journal of Bacteriology, October 2001, p. 5589-5598, Vol. 183. link
 * "Whole-Genome Expression Profiling Defines the HrpL Regulon of Pseudomonas syringae pv. tomato DC3000, Allows de novo Reconstruction of the Hrp cis Clement, and Identifies Novel Coregulated Genes", Adriana O. Ferreira et al, MPMI Vol. 19, No. 11, 2006, pp. 1167–1179. DOI: 10.1094/MPMI-19-1167.
 * "Transcriptional Regulation of Components of the Type III Secretion System and Effectors in Pseudomonas syringae pv. phaseolicola", R. Thwaites et al, MPMI Vol. 17, No. 11, 2004, pp. 1250–125.
 * "Transcriptional regulation of the Pseudomonas aeruginosa type III secretion system", Timothy L. Yahr1* and Matthew C. Wolfgang, Molecular Microbiology (2006).

Day 1 (13 July 2007)
James and Jerry
 * 1) SyntheticBiologyReview pmid=16978856

Day 2 (16 July 2007)
Cheuk and Maira
 * 1) SyntheticBiologyReview pmid=16738572
 * [[MEDIA:Icgems_ronweiss.ppt|PPT]]

Questions about this paper:

Q: How easy is it to modify part via directed evolution? A: Difficulty is beyond the scope of the project.

Q: Any tips for multi-module design ? A: Try to keep the number of modules small.

Day 3 (17 July 2007)
James and Lucas
 * Idempotent Vector Design for Standard Assembly of Biobricks [Tom Knight,MIT Artificial Intelligence Laboratory, 2003]

Questions about this paper:

Q: How can we determine whether a plasmid has the insert? A: If the insert disrupts a gene in the plasmid that codes for an antibiotic resistance, the bacteria with the correct recombinant plasmid will be susceptible to the antibiotic on the replica plate.