Erickson:Colony PCR

Colony PCR 1 μL Platinum Taq® 30 μL 10X PCR Buffer (-MgCl2) 5 μL Primers (forward and reverse) (10 μL total) 6 μL 50 mM MgCl2 5 uL PCR nucleotides 198 μL H2O ''250 μL volume total. Adjust volume according to needs.'' - For colony PCR, scrape small volume of colony and add to PCR Master Mix (20 μL)
 * PCR Master Mix