IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/13

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7/13/10
9:50 AM

Transformation

100ul cells, 10 ul ligation product (ArsR-pSB1A2 7/12/10). Electroporate, add 1 mL LB, recover for 1 hour at 37°C.

Plated 200 ul on Amp plates at 11:05 AM

12:00 PM

PCR Per Reaction: 19.2 μL mastermix, .4 μL F primer, .4 μL R primer.

samples

LamBF1R

LamBF2R

ArsR Fusion

ArsR

pArsRF1R1

pArsRF1R2

pArsRF2R1

pArsRF2R2

Negative control (no primers)

Program

2:35 PM

Gel




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