User:Morgan L. Paull/Notebook/C.Dog V1.0 - T7 Promoter/2011/07/07

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] C.Dog T7 Promoter Project
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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GFP vs RFP

 * E06 and E07 a little funky, not lining up well with RFP. E06 has almost no matching, E07 only a little strange, not very far off.
 * R3.2 should be RFP, are in fact in a GFP backbone. However, R3.1 is on RFP, so it should be fine.
 * R7.1 sequenced weird, R 7.2 has some mutations
 * R6.1 no match to RFP, but R6.2 is ok
 * R10.1 is gibberish, should be RFP. However, R10.2 is RFP, so it should be fine.
 * R22.1, R22.2, R23.1, R23.2, R24.1 and R24.2 are in GFP backbones
 * B04 (G4.2) does not map onto GFP at all. However, G4.1 does, so I shouldn't have a problem here.
 * B04 (G4.2) matches well to RFP.
 * G4.2 is in RFP, but it should be ok, because G4.1 is ok (ie, in GFP).
 * Otherwise, all G's are ok

R2.2 ok R1.2 ok


 * R6.1 sequenced weirdly. However, R6.2 lines up well with RFP, so it should be fine.

Questions on:


 * R10.1
 * R10.2 (SNP ok?)
 * R6.1

Need to re-do in some way:


 * R7 (7.1 sequenced weird, 7.2 has some substitution mutations)
 * R16 (no colonies)
 * R17 (no colonies)
 * R18 (no colonies)
 * R22 (both GFP)
 * R23 (both GFP)
 * R24 (both GFP)


 * might be worth re-doing R20, R10

T7 promoter

 * present in all R's
 * present in all G's

Makoff RBS part 1

 * present in all G's
 * present in all R's

Good sequences

 * All G clones are good, except for G4.2
 * R1.1 (R1.2 ok - SNP)
 * R2.1 (R2.2 ok - SNP)
 * R3.1
 * R4.1 or R4.2
 * R5.1 or R5.2
 * R6.2 (R6.1 gibberish)
 * R8.1 or R8.2
 * R9.1 or R9.2
 * R10.2 (ok - has SNP)
 * R11.1 or R11.2
 * R12.1 or R12.2
 * R13.1 (R13.2 ok - SNP)
 * R14.1 (R14.2 ok - SNP)
 * R15.1 (R15.2 ok - SNP)
 * R19.1 or R19.2
 * R20.1 or R20.2 (minor SNPs in both)
 * R21.1 or R21.2


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