User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/07/30

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Friday 30th July 2010
The plasmid digestions are not suitable for ligation, one is degrading the plasmid so I should throw it away but I’ll do it just in case, the other is partial. For both I raised the volume up to 50 ul and left it to cut all weekend!

Reactive.............1x [ul]

DNA..................---

BSA...................---

Buffer2..............+ 2

H2O..................+ 14

EcoRI-HF..........+ 2

PstI...................+ 2

Total..................50


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