KAD072406-Construction of pSB1A2-J01129-J01007

[pK2(presumably on pSB1A2)]+[key3] Digest pSB1A2-J01007 [AlwnI/SpeI, Neb2, Large-1593] Paste into pSB1A2-J01129 [AlwnI/XbaI, Neb2, Small-710bp] Product is pSB1A2-J23024 this construction has been abandoned because it turns out that we dont have the basic part for key3. the construction file here describes the construction of [pK2][Ptet][key3] which is stupid.