IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/04/Shan Shen

Prepare the samples of the bi-stable cells:

9:30 Prepare the first sample, the residual was diluted for a fold. Prepare one sample with the same method each hour.

17:30 Check those samples using the flow cytometer. There aren’t strong red and green controls, so that the results are not convincing.

19:30 Check the parts:

22:30 Transformation for the parts above.

1:45 The incubation was started.

2:00 Pick the pZSA GREEN and RED for incubation, 3-13M as a red control, 2-9B as a green control, 1-1D as a negative control.