BCH081006-Construction of pJ23006-J23061 key/tRNA

 Overlap extension KB032/KB033      (141bp, XbaI/PstI) Sub into pJ23006                   (XbaI/PstI) Product is pJ23006-J23061, screen with XhoI/AlwNI

KB032 cctagtctagagggagagatgccggagcggctgaacggaccgggatccacCTCGAGctaagcttcccg KB033 ctgaactgcagcggccgctactagtatggcggagagagggggatttgaacccccggtagagttgcccctactccgggaagcttagCTCGAGg