User:Alexander S Mikheyev/Notebook/P. rapida partial genome/2009/11/16

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precipitation

 * 0.5 volume (125 ul) of 7.5M NH4OAc, mix,
 * then add 2.5 volumes (950 ul) of room temperature 95% ethanol
 * 1 hour @ -20C
 * spin max speed 15 min
 * wash with 200 ul cold 80% ethanol
 * spin 5 min max
 * dry, re-suspend in 15 ul water
 * there is about 15 ul of gel left
 * looks like crap on the nanodrop (huge peak at 230)

PCR of ligated product

 * 6 ng/ul (61 fmol/ul) ==> 180 ng total yield
 * frozen at -20C