User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/29

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Tuesday 29th June 2010
I checked the plasmid extractions from the previous ligations on an agarose gel and came out as follows:

1º and 8º lanes are the DNA ladders

2º and 3º lanes are cI plasmid + GFP # 1

4º and 5º are cI plasmid + GFP # 2

6º and 7º are cI-PCR + GFP + Plasmid 18

There is plasmid on all colonies tested.

I still need to check the length of the plasmids inserts to be sure the ligations are the ones that I want.

For that I digested the plasmids with EcoRI and PstI as follows:

Reactive...1x [ul].....7x

DNA.........5.............-

BSA..........1.............7

Buffer2.....2............14

H2O.........10..........70

EcoR1......1.............7

PST1........1.............7

Total........20..........140


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