Dahlquist:Protocols

Lab Organization

 * [[Media:DahlquistLabSafetyCitizenship.pdf | Laboratory Safety and Citizenship Rules]]
 * [[Media:Dahlquist_StudentContactInfo.pdf‎|Student Contact Information Form]]
 * [[Media:DahlquistLabNotebookGuidelines.pdf | Guidelines for Paper Laboratory Notebooks]]
 * [[Media:DahlquistLabCheck-outProcedure.pdf | Check-out Procedure for leaving the lab]]
 * [[Media:DahlquistLab_TemperatureLogTemplate.pdf | Temperature Log Template]] (for recording daily temperatures of freezers, incubators, etc.)

Wet Lab Protocols

 * DNA Microarray Protocol
 * [[Media:DahlquistLab_Temperature-sensitiveGrowthProtocol.doc | Testing Yeast Strains for Temperature-sensitive Growth on Solid Media]]
 * [[Media:DahlquistLab_YEPDliquidmedia.pdf | YEPD Medium]]
 * [[Media:DahlquistLab_YEPDagar.pdf | YEPD Plates]]
 * [[Media:DahlquistLab_AutoclaveInstructions.pdf | Autoclave instructions]]
 * [[Media:DahlquistLab_ColonyPCRProtocol_YeastDeletionStrains.doc | Yeast Colony PCR]]
 * For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
 * To download the file of primer sequences and product sizes, right click on this link and choose "Save target as" or "Save link as". The file is a tab-delimited text file that then can be opened in Excel.  The file uses the systematic name (ORF ID) for the genes which can be looked up at SGD.
 * Protocol for quick purification of genomic DNA from yeast for PCR (Looke et al. 2011, Biotechniques 50(5): 325–328); haven't tried this yet, just bookmarking it here.
 * [[Media:ScannerInstructions.doc | Scanning instructions GenePix 4000B]]
 * [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]]
 * Dahlquist:Transformation Protocol for E. coli Chemically Competent Cells
 * Kodak Gel Logic 100

Real-time PCR

 * Bio-Rad CFX Manager Plate Quick Guide
 * ABI TaqMan Gene Expression Master Mix Quick Reference Card
 * ABI TaqMan Gene Expression Master Mix Protocol
 * ABI Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR
 * 10 Tips for consistent real-time PCR
 * NormFinder software
 * geNorm software

Dry Lab Protocols

 * Dahlquist:Microarray Data Processing in R
 * GenMAPP & MAPPFinder Protocols from the Spring 2010 Bioinformatics Lab course.
 * GenePix Pro Software Protocol
 * [[Media:GenePix.pdf| GenePix Pro Manual 6.0]]
 * Edge Software Protocol
 * [[Media:Edge_manual.pdf | Edge Manual]] (Note: I posted this because I could no longer find a link to it on the web.)
 * Dahlquist:Wiki Checklist
 * Changing Drive Letters on Windows XP and 7
 * Converting Excel tables into wiki formatting
 * Blackboard 9.1 Manual

Reagents

 * [[Media:DahlquistLab_Primers.xls | List of oligonucleotides/primers in Dahlquist Lab]]
 * Yeast Systematic ORF Deletion Project
 * List of Yeast Deletion Project Primers
 * pORF yeast primers FTP site
 * HP High-Gloss Photo Paper C6565A (for CS poster plotter)