User:Fermenter User/Notebook/BROM C3

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM C3 (F6)

Day 0 (Dec 1 Mon):
Change #1: Media composition: JR (C): Expect pH change (will require more Base)             12/1/2208 (10:30) Bring media to fermenter room            12/1/2008 (11:18) Assemble bioreactor      12/1/2008 (11:18) Add 2 ml of Antifoam <-- Vincent's Exp (BROM B8) showed no foam with 5-ml. 12/1/2008 (11:30) Start Sterilization (Liq 45 min) 12/1/2008 (13:10) Cooling of bioreactor (Temp setup) 1 L/min air, 250 rpm 12/1/2008 (13:14) Start Fermentation Software for Fermenter #6 <-- shutoff 12/1/2008 (13:50) Connect Acid (495 ml) and Base (390 ml), prime pumps Start pH control pH of media was 6.88 at 30C. 12/1/2008 (13:53) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 12/1/2008 (13:56) Set dO2 12/1/2008 (13:56) Completion of bioreactor set up: Ready for inoculation 12/1/2008 (13:58) Start Fermentation Software for Fermenter #6 Record 0:00 Change #2: Minimum rpm is requested: rpm set at beginning:250 <--Same as Vincent's last EXP.                12/1/2008 (14:15) Inoculation: inoculation volume (300 ml) Fermentation 0:00             12/1/2008 (14:30) Sampling  Fermentation 0:15 Cell weight (26 mg), Activity ( 34%, x100), Activity ( 5%, x1000)             12/1/2008 (18:00) Sampling  Fermentation 3:45 Cell weight ( 75 mg), Activity (48%, x100), Activity ( 0%, x1000)

Day 1 (Dec 2 Tue):
            12/2/2008 (8:00) Sampling  Fermentation 17:45 Cell weight ( 195 mg), Activity (77%, x100), Activity ( 21%, x1000)            12/2/2008 (8:03) Fermentation 17:48 Acid (485 ml) Base (280 ml) Bioreactor picture taken

12/2/2008 (9:00) Fermentation 18:45 Based on biocontroller record, O2 tank ranout 12/2/2008 (9:27) Fermentation 19:12 New O2 tank opened

            12/2/2008 (12:30) Sampling  Fermentation 22:15 Cell weight ( 233 mg), Activity (75%, x100), Activity ( 21%, x1000) <Natasha>            12/2/2008 (16:00) Sampling  Fermentation 26:15 Cell weight (170 mg), Activity ( 82%, x100), Activity ( 48%, x1000)

Day 2 (Dec 3 Wed):
<Natasha>            12/3/2008 (8:05) Sampling Fermentation 41:50 Cell weight ( 172 mg), Activity ( 87%, x100), Activity ( 59%, x1000) <Sung-Hye>           12/3/2008 (8:10)     Fermentation 41:55 Acid (450 ml) Base (90 ml) Increase rpm to 450 <Natasha>            12/3/2008 (12:15) Sampling Fermentation 46:00 Cell weight ( 156 mg), Activity ( 88%, x100), Activity ( 55%, x1000) <Natasha>            12/3/2008 (16:00) Sampling Fermentation 49:45 <-- remeasured Cell weight (356 mg), Activity (49 %, x100), Activity (6 %, x1000) <Sung-Hye>           12/4/2008 (16:32)     Fermentation 49:45 Terminate Fermentation


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