User:Anthony Salvagno/Notebook/Research/2009/03/27/Plasmid Extraction and Digestion

I'm in a quirky mood today and so... I want to mix it up if you would like.

Part 1
I followed a Qiagen miniprep protocol. It worked well. Last time I took cells from 6 of the 20 colonies and grew more cells. Then I followed this protocol to extract the plasmid DNA. Next I ran a nanodropper on each of the 6 different tubes (remember each tube should contain a different random fragment). My results:

Tube 1 - from culture 6 - 107.9 ng/ul Tube 2 - from culture 7 - 107.7 ng/ul Tube 3 - from culture 14 - 112.9 ng/ul Tube 4 - from culture 15 - 108.7 ng/ul Tube 5 - from culture 16 - 132.2 ng/ul Tube 6 - from culture 17 - 92.5 ng/ul

Good job me.

Part 2
Then I set up a digestion reaction. Here it is.

Part 3
Gel setup so we can see whether we got working ligations/insertions. Picture coming soon.