IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/04/19

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Wet lab meeting

 * Our goals for the following weeks are:

-Mariana:

1. Do primers for the blue promoter.

2. Do LB plates with kanamycin and ampicilin.

3. Obtain luciferase by PCR or band purification.

-Jorge:

1. Extract regulatory region of YcgF (blue promoter) by PCR.

2. Extract reporter plasmids by PCR.

3. Transform reporter plasmids.

 -Augusto

1. Extract double terminator [| BBa_B0015] by plasmid extraction.

2. Do a restriction assay for the plasmids.   -Zepeda 1. Transform luciferase into E. coli strains.  2. Extract plasmids.


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