IGEM:MIT/2005/Notes

Divide & Conquer

 * Sensor - External signal -> Internal Signal
 * Ray
 * Jessica


 * Signal-Processor - Internal Signal Processing
 * Annie
 * Jenny


 * Actuator - Internal Signal -> Output
 * Maxine
 * Will


 * System Integration - What is this whole system doing?
 * Jen


 * Read EVERYTHING by KT
 * Summarize relevant papers (Journal Club)
 * Don't make us look stupid.
 * Please stock BUG Lounge with food. We are hungry college students
 * We need to edit the Registry easier to read. More details.
 * Plan assembly of test constructs
 * J07000: R0053, Q04121, Q04510, E0430
 * J07001: R0053, Q04121, Q04400, E0430
 * J07002: R0040, Q04121, Q04510, E0430
 * J07003: Z0251, Q04121, Q04510, E0430
 * J07004: Z0251, Q04121, Q04400, E0420
 * Place order with Registry for parts to be synthesized
 * As said in above bullet.
 * We need R0010. Has CAP binding site.
 * Prepare FACS samples for Thursday
 * We will get cells from Drew's Lab.
 * Order new parts from Blue Heron
 * Specify on Registry!
 * insulin - Karen Talmadge
 * export tag
 * etc?
 * Order supplies
 * Make stocks

Test Constructs
How are we going to test our system in its intermediate, and also its final stages?
 * 1) Flow cytometry
 * 2) Beta gal assay
 * 3) Microscopy (detect gfp, bgal, etc)
 * 4) Western blot (measure protein levels)
 * 5) insulin assay?
 * 6) use cells as indicator - as cell-cell signalling system!
 * 7) *insulin receptor (research this)
 * 8) *sender/receiver
 * 9) *feedback (incorporate insulin output into regulating glucose - ultimately CAP - input)
 * 10) RT-PCR
 * 11) *used to measure RNA levels
 * 12) Northern Blot
 * 13) *used to measure RNA levels

June 15, 2005

 * Familarize
 * Glucose receptor (what is the signal cascade?)
 * plasmid?
 * chromosome?
 * Insulin plasmid (read the paper about it)
 * How is insulin produce from E. coli?
 * Tags
 * Sequence of insulin
 * Apoptosis in E. coli?
 * List of things to get for lab
 * Insulin is in 2 chains, so we need to look at how we can regulate it. In vitro?