Ketner Lab

Current Projects
Adenovirus Vaccines
 * Malaria
 * HPV

Ubiquination

Protocols

 * Adenovirus CaCl transfection
 * Generating hexon mutants in E. coli
 * Generating fiber mutants in E. coli
 * Generating late gene expression mutants in 293 cells
 * Purifying adenovius (banded stocks)
 * Titration of viral stocks (calculate pfu)
 * Calculate MOI
 * Protein preps of infected 293 cells for westerns
 * Western Blot
 * P32 label DNA
 * Quantitate number of virions in CsCl virus preps

Antibodies

 * anti-HA
 * anti-Fiber
 * anti-Late serum
 * anti-NANP hybridoma supernatant

Links
Gary Ketner

JHSPH Magazine