Griffitts:Trizol purification

Procedure
When ready, proceed to DNAse treatment
 * Grow up Rm1021 (B100) to OD600 = 0.9–1
 * Place the cells on ice to inhibit further growth
 * Centrifuge 1 mL of cells for 1 minute
 * Dump and tap
 * Add another 1 mL of cells and centrifuge for 1 minute
 * Remove supernatant and store pellet on ice DUMP AND TAP?
 * Make TED solution
 * Add 50 μL of TED solution to the pellet while on ice
 * Mix well
 * Add 50 μL of 1% SDS to the pellet
 * Immediately vortex 1 second
 * Place in 68°C heat block for 3 minutes
 * Add 1 mL trizol (in fume hood)
 * Vortex until pellet is broken apart
 * About 1 minute
 * Add 200 μL chloroform (in fume hood) to tube
 * Shake vigorously
 * Let stand for 5 minutes
 * Centrifuge at full speed for 2 minutes
 * Carefully remove 600 μL of the upper phase to a new tube
 * Add 600 μL of isopropanol (in fume hood)
 * Invert to mix
 * Place on ice for 10 minutes
 * Centrifuge for 5 minutes
 * Dump and tap
 * There should be a white precipitate at the bottom
 * Wash with 300 μL of 75% ethanol
 * Invert to mix
 * Centrifuge
 * Carefully remove ethanol
 * Store pellet in the -20°C or -80°C freezer

TED
Keep on ice
 * 880 μL ddH2O
 * 40 μL 0.5 M Tris pH 7.5
 * 40 μL 0.25 M EDTA ph 8
 * 40 μL 1 M DTT (from -20°C stock)