Shreffler:Notebook/Alex Daily/2011/06/01

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Buhlmann Assay pilot 4
Ran Buhlmann assay using Kadlik buffy, drawn 6/1/11. Used five dilutions each of Milk and Peanut allergen (1.00, 0.20, 0.040, 0.0080, 0.0016 µg/mL)

Followed Buhlmann protocol with following differences:
 * Incubation step in incubator rather than water bath
 * Included anti-IgE Ab positive control

Observations/notes
Procedure went great. Especially robust basophil gate and degranulation to positive controls. No response to allergens.


 * [[Media:Image-BuhlmannAssay_20110601_Kad.pdf]]


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