User:Mark X. Ling/Notebook/Micb 323/2009/03/31

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ECL
1o incubation: 1:09->2:07 3 washes 2o incubation:2:20


 * as expected no bands showed up on Crystal's xray. No iNOS on PBS, and there is too little iNOS in the total protein from the lysate to be detected.
 * only one lane of LPS induced had iNOS detected.
 * used 10ng/mL only, so rest may not have been too induced
 * may have been problem with the immunoprecipitation, not enough iNOS concentrated for the rest.
 * if any was to have a band, LPS is expected => had most NO in the NO assay

Nitric Oxide Assay

 * incubated at: 1:56 -> 2:09

Multititer plate map
 * note the change of the sample placement, LPS samples and IFNg treatments switched places
 * there are no 96hr samples

NaNO2 Standard
 * 2mg tablet dissolved in 1mL water ==> solution is then diluted 1/100
 * stock concentration = 289.9uM

My results for Nitric Oxide Assay

Post lab comments

 * NO assay result were not good
 * too much bubble, so there were spots with very high reading
 * no reliable enough so used Crystal's data


 * xray result is okay, at least saw a band.
 * indicates ECL prcedure went okay.
 * immunoprecipitation mistakes, maybe only the 48 hours LPS went well


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