Koch Lab:Protocols/Sequences/Unzipping oligos

Top adapter 1a - TCGA, ATCG
Oligo #1: Top adapter 1a - TCGA, ATCG Sequence 5' to 3': TCGAGTCTGAATTCTAATGTAGTATAGTAATCCGCTCATCG Modification: 5' phosphate

Bottom adapter 1a - Biotin
Oligo #2: Bottom adapter 1a - Biotin Sequence 5' to 3': GAGCGGATXACTATACTACATTAGAATTCAGAC Modification: X = biotin-dT

Bottom adapter 1b - Biotin, CATG
Oligo #3: Bottom adapter 1b - Biotin, CATG Sequence 5' to 3': GAGCGGATXACTATACTACATTAGAATTCAGACTCGACATG Modification: X = biotin-dT

Hairpin Adapter 1
Oligo #4: Hairpin Adapter 1 Sequence 5' to 3': GAGCGGATXACTATACTACATTAGAATTCAAACTCGAGAGAGTAATCTCTCGAGTTTGAATTCTAATGTAGTATAGTAATCCGCTCATCG Modifications: X = biotin-dT

Kochlab note: we also have slightly modified biotinylated versions of the above that are missing the T (due to an error in the way we ordered them).

Hairpin Adapter 2
Name:             Hairpin Adapter 2 -- NotI Scale:            1 micromol Sequence 5' to 3': GAGCGGATXACTATACTACATTAGAATTCAAAGCGGCCGCAGAGTAATCTGCGGCCGCTTTGAATTCTAATGTAGTATAGTAATCCGCTCATCG Modification:     X = biotinylated dT Purification:      PAGE Note: This oligo is a self-complementary hairpin.

Top Adapter -- BstXI / SapI
Name:             Top Adapter -- BstXI / SapI Scale:            200 nmol Sequence 5' to 3': 5’-Phosphate- GCTGTCTGAATTCTAATGTAGTATAGTAATCCGCTCATCG Modification:     5'-phosphate Purification:     OPC (Unless you think PAGE would significantly improve purity) Mfold verification of hybridization with Bottom Adatper 1a: Image:New Top Strand Adapter.png

Sap Cap AGC
Name:             Sap Cap AGC Scale:            200 nmol Sequence 5' to 3': 5'-Phosphate- AGCGGCGACCTAGCGTAAGCTAGGTCGCC Modification:     5'-P Purification:     OPC Image:09Aug28-18-38-04 1 (1).png