User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/18

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

01/18/10

 * &#x2713; Cultures: KAH94, 95/JDS49 (not 47!) (6 each); KAH94, 95/JDS48 (6 each)
 * &#x2713; Minipreps: KAH118/MV2 (4)
 * &#x2713; Assemblies: KAH139, 140, 141

Minipreps > Check with E/P digests

--> None have correct size for MV2 vector. Pick 12 more colonies.

Assemblies
 * 1) KAH139: HPK-Kstop(KAH103)/(E/S)/546 + (1) KAH137/(E/X)/~5593
 * 2) KAH140: HPK-K(KAH104)/(E/S)/540 + "
 * 3) KAH141: HPK-K(KAH104)/(E/S)/540 + (2) KAH138/(E/X)/~5181

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations


 * }