IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/09/03

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Melody, Eric

 * Autoclaved 30ul pipette tips

Biofilm Protocol Day 4

 * 0.1% Crystal Violet stain on plates 100831M24 + 100831M26

Biofilm Protocol Day 3

 * Took plate 100901M28 @ 8:06 p.m.
 * Took plate 100901M30 @ 10:15 p.m.


 * No contamination


 * 3 x 300ul PBS wash of plate 100901M28
 * heat fixed @ 8:34 p.m.


 * 3 x 300 ul PBS wash of plate 100901M30
 * heat fixed @ 10.46 p.m.

dspB Track

 * Plates taken out at 11am
 * Lawn of colonies on all 6 plates including transformants with ccdB chlor vector. Is ccdB resistant to chloraphenicol now???

Gel verification on RD
Gel orientation: Machine conditions: 0.5x TBE buffer, 100V, 60min Results:
 * Protocol: biobrick "digest" protocol
 * Changes: load ~17uL into each well; 10uL for ladder

Gel extraction
ccdB chlor gel weight: 0.0790g RFP chlor gel weight: 0.0878g
 * Using Invitrogen PureLink Quick Gel Extraction Kit (protocol inside kit)

Nanodrop:
 * [ccdB chlor] = 5.5ng/uL
 * [RFP chlor] = 5.2ng/uL

Transformation

 * Protocol: SOP
 * Put in 37C incubator @ 6:47pm
 * Plates:
 * 1: ccdB no his
 * 2: ccdB his
 * 3: ccdB no his Alina
 * 4: ccdB his Alina


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