IGEM:IMPERIAL/2006/project/primers/Cre Lox

Primer 1


 * Primer 1 is the same as the sense streand and consists of
 * Restriction Sites – Lox Site – Stop Codon – First 20 bases of part B0021

GAATTCGCGGCCGCTTCTAGAGCGATAACTTGGTATAGCATACATTATACGAACGGTATGAAAATAATAAAAAAGCGG

Primer 2


 * We need both the sense strand and the anti-sense strand for this primer
 * 31bp into part B0021 (long due to a run of T and A)
 * 22bp into part I13521

Sense Strand :

CGGATTAATAATCTGGCTTTTTATATTCTCTTATAAACGCAGAAAGGCCCACC

Antisense Strand:

GGTGGGCCTTTCTGCGTTTATAAGAGAATATAAAAAGCCAGATTATTAATCCG

Primer 3


 * Primer 3 contans a section complementary to the sense strand of the construct and a Lox site and some restriction sites. It was made by copying the end of the desired sense strand and making it complementary and reversing it.

CTGCAGCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTAACTCCCTATCAGTGATAGAGATTGACATCC

For further information on incorporation [here]