IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/28

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Entry title

 * Miniprep performed on the part J13002 which was inoculated the earlier night.
 * Decision to repeat all the constructions using 2A assembly.
 * The following parts were digested
 * J23119 (S/P)
 * J23119 (S/P)
 * R0010 (S/P)
 * K117005 (S/P)
 * B0014 (E/X)
 * S03879 (X/P)
 * E0420 (X/P)
 * E0420 (X/P)
 * K145151 (X/P)
 * K145151 (E/S)


 * These digestions were done with the purpose of constructing the following composite parts:
 * J23119 + S03879 -> 001
 * K145151 + B0014 -> 018
 * J23119 + E0420 --> 019
 * R0010 + E0420 ---> 502
 * K117005 + K145151 ---> 503
 * Gel Order:
 * Row 1: J23119; J23119(S/P); R0010; R0010(S/P); K117005; K117005(S/P); B0014; B0014(E/X); LADDER; S03879; S03879(X/P); E0420; E0420(X/P); K145151; K145151(X/P); K145151(E/S)
 * All digestions worked except for E0420.
 * All digestions worked except for E0420.


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