IGEM:UC Berkeley/2006/KCMSmallScale


 * 1) Grow cells in ~4 mL LB until cloudy
 * 2) Put on ice
 * 3) Transfer 1mL into an eppendorf tube on ice, let cool
 * 4) Centrifuge full speed for 30 sec, toss out supernatant
 * 5) Resuspend in 90uL of TSS solution
 * 6) Add 10uL KCM
 * 7) Add 1uL plasmid DNA
 * 8) Let sit on ice for 10min, heat shock 90 sec at 42, incubate and/or plate