User:Matthewmeisel/Notebook/2006-6-13

DNA nanostructure reaction PCR
 * order of lanes: 1) 1kb ladder, 2) full rxn (oligos + scaffold), 3) just scaffold, 4) just oligos
 * run on 2% agarose gel
 * appears that reaction was successful: [[Media:Tc_mm_pt_lh_folding_0613.jpg|gel image]] (leftmost four lanes) shows expected results
 * assembled nanostructure runs slightly faster than scaffold, oligos appear as a smear of short DNAs

Transformation of standard components
 * results of plated bacteria: all three plates showed colony growth, negative control did not
 * colony selection and amplification
 * 1) Seven culture tubes were filled with 5 mL LB medium and 50 &mu;l of 5 mg/mL ampicillin
 * 2) Colonies were selected from the three plates (three from R0010, two from E7104, two from E0241) and were transferred into respective culture tubes with a sterilized pick
 * 3) Culture tubes were incubated, with shaking, at 37&deg;C for 18 h.
 * 4) Plates were stored at 4&deg;C.