User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/28

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 * style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] WiFi coli: A communicolight system
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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There it goes!
         - H2O -> 13
 * Today, I checked that the restrictions had the appropriate size, which can be observed next:
 * The lanes indicate that ligations were empty, so I will need to re-ligate them :(.
 * To fix this problem, I was planning on ligating using a type of three antibiotic assembly. However, when I ran the gel to check the insert and vector relation, I saw nothing where the insert (luciferase) should be :(.
 * Luckily, I found a mutated luciferase stock, so I will leave a restriction for a total of 30μl as follows ON at 37°C.

- Buffer 3 --> 3μL 

- BSA > 1μL <br/ >

- DNA > 10μL <br/ >

- XBA I -> 1.5μL <br/ >

- PST I --> 1.5μL<br/ > <br/ > <br/ >


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