July

=Tahoura Samad 13:22, 2 July 2010 (EDT)=
 * most of my transformations with the new competent cells were successful.
 * competent cell checks for new 1 and new 6 looked good.
 * miniprepped IBB as CA lefty, CK lefty and CK righty, and sent out for sequencing with ca998.

=Tahoura Samad 13:38, 1 July 2010 (EDT)= Kan LB Cam Amp Spec
 * controls for JTK030 looked good with no growth on Kan, Amp, Cam, good growth on LB, and a little growth on Spec, which is the normal result because of mutations in the bacteria. Those are labeled with double black stripes, in a box in the back of the -80 in the 2nd row from the top.
 * picked and grew up the results of PCA for the new nuclear localization tag. They had plenty of colonies, with only one background green colony. Will miniprep them tomorrow.
 * did small scale competent cell prep of 1 and 6.
 * did the following transformations (1old:2348), (5 old:2291) (6 old:2291) (6 new:2291),(3 old :2348) (3 old: 2349) (3 old:2291) (2 old:2348) (2 old: 2349)(2 old:2291) (2 new:2348) (2 new:2349) (2 new: 2291), (1 new:2331) (1 new:2333) (1 new:2348) (1 new:2349) (1 new: 2291) (6 new 2348) (6 new:2349), (IBB into lefty).
 * redid assembly of stage 0 of life act but with 2.4 μL of each DNA and 1.2 μL of 5x NEB2.