User:Torsten Waldminghaus/protein gel western

Recipe for buffers

 * Store at rt.


 * Store at -20°C
 * Should be 15% of endvolume (for example 30 μL sample + 5.3 μL Loading buffer

Western

 * activate the membrane (for example Immobilin -P, Millipore) with methanole and then equilibrate with water.

Semi dry blot assembly

 * 1) 2 filter papers with Anode buffer I
 * 2) 1 filter papers with Anode buffer II
 * 3) Membrane
 * Gel
 * 1) 3 filter papers with Cathode buffer


 * Run 30 min at 15V