Klapperich Lab:Notebook/Lab Meeting Notes/2008/10/14

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14 October 2008 Lab Meeting Agenda
† MinCheol's research report today.

† Announcements † Flu R01 † SEPSIS Project - CIMIT Bird Flu †RNA project † COBRA † Valve Array/Valve building (FJ) † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR '''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ''' † RCA † Senior project † F31: Cochlea † Silica Optimization (Lambda):
 * JZ and JD out this week at mTAS.
 * TO DO More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly? YES. Cathie will amend.
 * Plaque assay status?
 * TO DO Cathie STILL STILL has to submit the progress report to INSPIR.
 * B sub in blood is done. Lets talk about comepleting the gram positive plots so they "match."
 * SEM image analysis of pore size. DONE. Thanks JZ
 * Run E. coli RT-PCR using primers for a genomic target.
 * Report DNA amounts along with the RT-PCR data for the Sepsis paper.
 * Jeff Braman will visit in early Dec.
 * Do dilutions. Send to Bioanalyzer.
 * Do dilutions. Send to Bioanalyzer.
 * Mark finish control circuit
 * Fluorescence microscopy available from 720. Try beads experiment. DONE.
 * Bacteria Experiment?
 * Jane, Cathie, Larry, Ranjith met Friday. JZ will try process with modified washes.
 * Waiting for fixture still from Melissa. Hopefully this week. UPDATE?
 * 9 Oct Shane says schematics are completed and would be emailed "right away". Have not received anything.  Will contact 14 Oct to figure out why.
 * Update?
 * Alex working on more toluene bonding issues.
 * Try Ticona material. (HM, JD, AG)
 * Still Still Still troubleshooting ??
 * Mold still not made. Mask potentially Wed AM. When mask is made.
 * Do preliminary E. coli experiment soon. Sonali will train.
 * Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
 * STAR-CD Renewal - January ($2K)
 * QQ will run it on a gel to check.
 * Run Gels for water Wash steps - show me and Sonali UPDATE?
 * Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE?
 * Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE?
 * Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE.
 * Cathie Still Still Working on Whitepaper.
 * Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources.
 * Haven't seen Teddy yet. What is up with him?
 * Ongoing. More concentrations..
 * Pressure testing on these channels when they are flowing.




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