User:Fermenter User/Notebook/BROM D4

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM D4

Day 0 (Jun 1 Mon):
           6/1/2009 12:30 Assemble bioreactor             6/1/2009 13:30 Bring media to fermenter room 6/1/2009 13:30 Add 10 ml of Antifoam            6/1/2009 13:40 Start Sterilization (Liq 45 min) 6/1/2009 15:16 Cooling of bioreactor (Temp setup) 6/1/2009 15:20 Start Fermentation Software for Fermenter #6 Record 0:00

Day 1 (Jun 2 Tue):
6/2/2009 8:30 Connect Acid (250 ml) and Base (400 ml), prime pumps Adjust pH to 7.0 F6: Base: 400 ml - 20 ml = 380 ml (total consumption) 6/2/2009 8:30 Start pH control 6/2/2009 9:00 Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 6/2/2009 9:00 Set dO2 6/2/2009 9:00 Completion of bioreactor set up: Ready for inoculation rpm set to 400 dO2 <40%

-  Frances, Do NOT forget to write down the amount of inoculation!!! ---                  6/2/2009 16:30 Inoculation: (300 ml) Fermentation 0:00

Day 2 (Jun 3 Wed):
            6/3/2009 (14:00) Sampling Fermentation 00:00 F6: Cell weight ( 78.9 mg/ml), Activity ( 76%, x100), Activity (15 %, x1000)

Day 3 (Jun 4 Thu):
            6/04/2009 (09:30) Sampling Fermentation 00:00 F6: Cell weight ( 121.9/ml), Activity ( 91%, x100), Activity (44%, x1000)             6/04/2009 (11:45) Sampling Fermentation 00:00 F6: Cell weight ( 206.00mg/ml), Activity ( 92%, x100), Activity ( 48%, x1000)             6/04/2009 (14:00) Sampling Fermentation 00:00 F6: Cell weight ( 235.1/ml), Activity ( 91%, x100), Activity ( 55%, x1000)             6/04/2009 (15:15) Sampling Fermentation 00:00 F6: Cell weight ( 250.4/ml), Activity ( 93%, x100), Activity ( 59%, x1000)             6/04/2009 (16:50) Sampling Fermentation 00:00 F6: Cell weight ( 188.3/ml), Activity ( 88%%, x100), Activity ( 50%, x1000)


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