User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/23

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Colony Screen

 * Poured off 1-1.5 mL of each colony into an eppendorf tube.
 * Added 15 μL of 0.1M IPTG to each 4 mL culture.
 * Shook at 37°C for 2.5 hrs.
 * Screened each culture for green fluorescence
 * High background autofluorescence
 * No colonies stood out


 * }