KAD072606-Construction of pSB1A2-I13450-pACSerZAGGA

[EcoRI][XbaI][tRNA][SpeI][PstI] Phusion PCR pACSerZAGGA [ca1000/ca1001] Digest pACSerZAGGA PCR product [EcoRI/PstI, NEB2, small-97bp] Paste into pSB1A3-I13450 [MfeI/NsiI, NEB2, large-2165bp] Product is pSB1A3-J23037