SBB09 Construct38

Construction of  basic part Bcr0001

PCR Ocr0001-F/Ocr0001-R on pAC-TetInvdBg (2735 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-Bcr0001 {a~Inv-short>} - Ocr0001-F      forward oligo for biobricking of {a~Inv-short>} ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg Ocr0001-R      reverse oligo for biobricking of  {a~Inv-short>} ccaaaGGATCCactggataccagcgatctgccgc

JCA: Ocr0001-F doesn't need to be quite so long: Construction of  basic part Bcr0001

PCR Ocr0001-F/Ocr0001-R on pAC-TetInvdBg (2735 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10030 {a~Inv-short>} - Ocr0001-F      forward oligo for biobricking of {a~Inv-short>} ccaaaGAATTCatgAGATCTAATTATGATGGTTTTCCAGC Ocr0001-R      reverse oligo for biobricking of  {a~Inv-short>} ccaaaGGATCCactggataccagcgatctgccgc