User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/09

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

07/09/11

 * &#x2713; Miniprep digests: check 183-177/MV8 clones #2, 4 (sequence verified)
 * &#x2713; Assemblies: KAH207, 208, 209/V0200; KAH210, 211, 212

Minipreps > Check with ApaLI digest

Assemblies
 * 1) KAH207/V0200: (1) KAH207/(N/S)/1836 + (4) V0200/(N/S)/4442
 * 2) KAH208/V0200: (2) KAH208/(N/S)/1884 + "
 * 3) KAH209/V0200: (3) KAH209/(N/S)/1932 + "
 * 4) KAH210: (5) KAH204/(E/S)/2328 + NLS myc stop/(E/X)/64 &#x2713;
 * 5) KAH211: (6) KAH205/(E/S)/1251 + "
 * 6) KAH212: (7) KAH206/(E/S)/1551 + "

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

--> Add to 30 μL DH5α Turbo; plate on 100 μg/mL Amp

--> * Re-do V0200 ligations using dephosphorylated vector


 * }