User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/04/08

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Dissected experimental testes in 1x PBS Solution. Store in eppendorf with 1x PBS until dissections are finished.
 * Two tubes, one for 1:1000 mip40 and another for 1:2500 mip40
 * Fix with 4% Formaldehyde PBS Solution
 * 30 minutes
 * Wash 2 x 5 minutes in 1x PBS-T
 * Incubate 30 minutes with PBS 0.6% Triton.
 * Wash once with 1x PBS-T
 * Block in 2% NGS
 * 1hr at RT or O/N at 4C
 * Incubate with primary antibody in 2% NGS
 * 1:1000 or 1:2500 mip40 B from Rabbit
 * O/N at 4C
 * Continued collecting virgins/males for stable control line.


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