User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/21

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03/21/10

 * &#x2713; Assembly: KAH142/V0201

Assembly
 * 1) KAH142/V0201: (1) KAH142/(N/S)/4571 + V0201/(N/S dp)/5157 &#x2713;

> Digest (Fermentas FD) --> Re-do KAH142 N/S digest

> Measure conc.'s

> Ligations

--> R.T./ ~10 min., Add to 50 μL Z-DH5α


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