User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/16

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Split cells for ELISA and secondary antibody Immunoblot
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * 24-wells (6x 750.000 cells per well)& 10cm dishes (8x 1000000 cells per plate) plated out (D9 P24)
 * probably too little cells on 24-wells due to miscalculation
 * Treated membrane with secondary antibody and obtained
 * a lot of unspecicific binding on membrane


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