IGEM:MIT/2006/Notebook/2006-8-28

completed

 * 1) re-ordered pms primers to PCR out the entire SA-generating device from plasmid pE3/pE3R
 * 2) miniprepped 12 30.Bat2.30.THI3 mutagenesis attempts -- ran gel: 2 correct
 * 3) *threw out bad ones
 * 4) analyzed sequencing:
 * 5) *one shuttle vector construct correct pUCP22-100-C
 * 6) *Q-E0840-A, Q-119-C, Q-199-C correct
 * 7) *threw out bad ones
 * 8) LCed new CHA0 pseudomonas strain, YYC912, pE3, pE3R
 * 9) try to figure out/organize mystery tubes/glycerols using digest/ligation sheets and plates
 * 10) *found lots of poorly labeled tubes (i.e. no date or description)