IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/01

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7/1/10
Meagan plated the cells transformed last night at 12:30 AM. We observed no growth on the plates this morning when we checked at 9:30 AM for both those plates and the PAL_pSB1A2 plate.

11:15 AM

Troubleshooting Gel

Ran ligations and digests on gels at 80V.





3:50 PM

Re-Ligating

J23100-Gas Vesicles-pSB1T3

J23119-gas vesicles-pSB1T3

Gas Vesicles-pSB1T3

incubate at room temperature for 10 minutes

inactivate at 80°C for 20 minutes

5:15 PM

Transformations

100 ul cells + 5 ul ligation product (J23100-Gas Vesicles-pSB1T3 or J23119-Gas Vesicles-pSB1T3). electroporate and add 1 mL LB, incubate at 37°C for 1 hour for recovery.

6:30 PM

Plating

Plated 20ul transformation product on 1/2 tetracycline plate, plated 200 ul on other half. Next day observed no growth.


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