User:Karmella Haynes/Notebook/BioBrick cloning/2010/06/01

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06/01/10

 * &#x2713; Assemblies: make MV8 and MV3

Assemblies
 * 1) MV8: MV1/(BsaBI/BstBI)/4605 + Hygro PCR/(BsaBI/BstBI)/1023
 * 2) MV3: MV7/(BsaBI/BstBI)/3919 + "

> Digests (NEB) --> Specific notes

--> 60°C/ 1 hr.; 65°C/ 1 hr.

> Measure conc.'s

> Ligations

--> R.T./ ~10 min.; Add 50 μL Turbo DH5α


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