User:Burak Yilmaz/Notebook/YR10 protein interaction/2009/08/31

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forward primer

 * 5' GGATTAATTAAATGGACTACAAGGACGACGATGACAAAGTCAAGCTTCTCGAGAATTCCGGTCGCAGCAGTTCGAAGAAAAAGAAGAA 3'
 * LENGTH: 77
 * GC CONTENT: 45.5 %
 * MELT TEMP: 69.9 ºC
 * MOLECULAR
 * WEIGHT: 23870.5 g/mole

reverse primer

 * 5' GGTGCGGCCGCTTAGCTCCAAACAGAGGGGTTTCTAAATTG 3'
 * LENGTH: 41
 * GC CONTENT: 53.7 %
 * MELT TEMP: 68.8 ºC
 * MOLECULAR
 * WEIGHT: 12681.2 g/mole


 * TTAATTAA PacI site
 * GACTACAAGGACGACGATGACAAA Flag tag
 * GCGGCCGC   NotI site


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