User:Morgan L. Paull/Notebook/Genetic Fence/2011/01/17

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Diagnostic Digest of GalUAS35s parts

 * Cut the two tubes I have each with xba pst and eco pst. The two tubes were an unlabeled one and one labeled as a ligation mix.
 * All reactions were 10μL, with 1μL FD Green buffer
 * Used 7μL of the gel extracted V0120 Backbone from yesterday, and 1μL of the 35s annealed oligos, at the concentrations listed below



1x insert concentration

 * 1μL 35s
 * 7μL BB
 * 2μL #3 Roche buffer
 * 10μL #4 Roche buffer
 * 1μL #5 Roche buffer

1/10x insert concentration

 * 1μL 1/10 35s
 * 7μL BB
 * 2μL #3 Roche buffer
 * 10μL #4 Roche buffer
 * 1μL #5 Roche buffer

1/100x insert concentration

 * 1μL 1/100 35s
 * 7μL BB
 * 2μL #3 Roche buffer
 * 10μL #4 Roche buffer
 * 1μL #5 Roche buffer

1/1000x insert concentration

 * 1μL 1/1000 35s
 * 7μL BB
 * 2μL #3 Roche buffer
 * 10μL #4 Roche buffer
 * 1μL #5 Roche buffer

Control

 * 1μL DH2O
 * 7μL BB
 * 2μL #3 Roche buffer
 * 10μL #4 Roche buffer
 * 1μL #5 Roche buffer


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