Talk:DIYbio:Demo

Instructions for the basic 3-step transformation:

Preflight checklist:
 * Box of competent cells on ice
 * Part Datasheets (includes part dna)
 * hole-punch tools (one per part)
 * sterile Luria Broth + ampicillin (LBamp)
 * 10 mL culture tubes
 * disposable pipets (1mL)
 * tape (for securing tubes to heat source)
 * 2 containers (one of water bath, one for ice bath)
 * microwave
 * thermometer


 * 1) punch out the dna spot from the datasheet of the part you want to use
 * 2) and drop the "dna chad" into a thawed tube of competent cells
 * 3) and leave this tube on ice for 30 minutes
 * 4) dip the tube into 42°C water for 1 minute (use the other container, the microwave, and a thermometer to heat the water.  Be precise!)
 * 5) put the tube back on ice for 2 minutes
 * 6) add 500 μL of LBamp
 * 7) tape the tube to a 37°C heat source, like the back of a computer for 1 hour.
 * 8) Dump onto an agar+amp plate - or - dump into 5 mL of LBamp in a culture tube.
 * 9) Incubate your plate or tube at the 37°C heat source overnight

see DIY placenta stem cells for graphic inspiration of this how-to