NanoBio:Protocols

=Nano Synth Bio=

General Molecular Biology
Current Protocols in Molecular Biology

Lab Practices

 * Keeping a Laboratory Notebook

Sub-cloning

 * Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
 * Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
 * Installing Vector NTI How to install Vector NTI, a free sequence editor
 * BioBrick & Biofusion Strategies for Making Parts An overview of how to make & combine parts.
 * 3 Antibiotic Assembly
 * Commonly Used Plasmids
 * Commonly Used Primers
 * PCR
 * Oligonucleotide Inserts
 * Overlapping Oligonucleotide Inserts
 * Site-Directed Mutagenesis
 * Restriction Digest
 * Ligation
 * Bacterial Transformation of Chemically Competent Cells
 * Prep for Electrocompetent cells
 * Bacterial Transformation using Electroporation
 * Plasmid Verification
 * Agarose Gels

Protein Expression & Purification

 * Bacterial Hosts for Overexpression
 * Test Induction & Purification
 * Protein Purification
 * Isolation of Periplasmic Fraction
 * Protein Gels
 * Cell Surface Binding Assay

Gene deletion with λ-red

 * Overview and Materials for λ-red
 * Primer Design
 * Protcol for gene knockout
 * Knockout/in

Genomic Integration into S. cerevisiae

 * Integration into Yeast
 * Verifying Genomic Integration

Lipid Bilayers

 * Making Unilamellar Vesicles
 * Preparation of Supported Bilayers
 * Cleaning SiO2 surfaces

Care of Synechocystis sp 6803

 * Liquid & Solid Growth Media
 * Guidelines from 2006 Harvard iGEM team

Primers

 * Biobricks & Biofusion Sub-cloning
 * Synechocystis sp. 6803
 * Shewanella oneidensis MR-1
 * Guidelines on Talking or Writing about your project
 * Microbial Respiration

Recipes

 * LB media & plates
 * Antibiotics
 * TSB media
 * BG-11 media
 * BG-11 plates
 * Supplemented M9 Media

Safety & Waste Disposal

 * Safety & Waste Disposal