IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output2009/06/16/Min Lin

10:00 Gel (1-12M and 1-12O product) Cut gel; Recycle the insert(about 1kb). Wash with 30 ul Elution buffer, store in -20.

21:37 Gel to compare the concentration of insert (1-12M and 1-12O) and the vector(T7 promoter cut with SpeI and PstI)

Ligation 16 centigrade overnight

Transformation( ligation product into Top10)