IGEM:Imperial/2010/Lab Work

LAB TEAMS
Our group has divided into three lab pairs in order to cope with the load of wet lab work. Each lab team will be responsible for the pathway indicated by the team's name while Wolfgang would act as a central co-ordinator. The co-ordinator would have the role ensuring all three groups are on schedule but also have a deep understanding of all components of the project. This will ensure that Wolfgang would be able to present the project with confidence in Boston (alongside with Ben). For summerized information on the overall progress click here.
 * XylE team (Maddie and Nicolas)
 * Vectors team (Kyasha and Kirill)
 * Surface Protein team (Harriet and Florian)

Lab supervisor: Wolfgang

DRY LAB WISH LIST
Experiments we wish that could be carried out for us Compare activities with the next one In vivo IPTG? -> compare the cultures that have less of it XylE -> maybe, but very noisy. Use robot to induce production and measure activities TEV -> FRET pairs with TEV link Yes -> Monitoring activity and then approximate the concentration. Remove the IPTG. Use robot Yes
 * Determine maximum concentration of sD that cells can produce – crucial! (in vivo):
 * Determine how much XylE we need to produce prior adding catechol (the threshold value to be determined). (in vitro then maybe in vivo)
 * Determine XylE and TEV production. (in vivo)
 * Degradation of XylE (in vivo or in vitro if the cell division is not taken into account)
 * Can we determine TEV kinetics (kcat, Km?) on XylE-GFP? (in vitro)