IGEM:MIT/2005/Experimental Timeline

1. Receive primers $$\rightarrow$$ Make biobricks, with these ../Techniques/ J07005 (phoA), J07006 (malE), J07012-14 (scFv 1-3), J07018 (FecA), J07019 (FecA promoter), FecR, FecI. 1.5 Make competent MC4100 cells, pour LB Kan plates, make freezer stocks of strains acquired from Registry 2. ToxR: Can begin assembly of R0040::B0030, for use in parts J07024-28 (see August wk. 1) 3. Input Experiments : Verify that flourescin oligo's enter cells. 4. Assemble fecA promoter with GFP --> test (after J07019 complete)
 * ../July wk 3:/

FecA: Fuse scFv with FecA? ToxR:
 * July wk 4:

ToxR: -Receive synthesized J07007 (ctx promoter), J07009 (ToxR') -Begin Assembly of: -J07011 (ctx promoter :: gfp reporter) -J07024-26 (promoter::RBS::ToxR'::scFv 1-3) -J07027-28 (Positive and Negative controls, promoter::RBS::ToxR'::PhoA/MalE)
 * Aug wk 1:


 * Aug wk 2:


 * Aug wk 3:


 * Aug wk 4:


 * Sept wk 1