IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/06

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6/27/10
9:10 AM

miniprep (promega kit):

lost about 2.5 mL of colony 2 for ArsR ligations again!

used new neutralization solution since there were chunks floating in the old solution.

concentrations:

 B0015 pSB1AK3: 200 ng/μL B0034 pSB1A2: 232.2 ng/μL pSB1C3 RFP: 279.1 ng/μL B0034-ArsRSDM1-B0015-pSB1C3 3 (1'): 339.2 ng/μL B0034-ArsRSDM1-B0015-pSB1C3 4 (2'): 252.6 ng/μL 

Glycerol stocks:

  pARsRF1R2 1' ISB218</li>  pARsRF1R2 2' ISB219 </li> </ul>

3:00 PM

Dilutions

Primers diluted to 10 micromolar

samples diluted to 20 ng/μL


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