McKinney:SodiumOleate

Sodium oleate solution
This is an additive to media used to culture M. tuberculosis.

Ingredients
(make 500mL)


 * 12.5mL 2M NaOH
 * 6.0mL oleic acid (=5g, since oleic acid specific gravity is 1g/1.2mL)
 * 481.5mL water

Protocol
Combine the above ingredients in a 1L flask. Stir with a magnetic stirbar until completely dissolved. Filter sterilize through a 0.22&mu;m pore filter. Store at room temperature wrapped in aluminum foil (oleic acid is light sensitive).