User:Karmella Haynes/Notebook/BioBrick cloning/2011/06/07

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06/07/11

 * &#x2713; Cultures/minipreps/digests: miR sensor test constructs (1 each of ###/196 set, 2 each of ###/177 set, 1 each of ###/198 set)

Minipreps > Check with E/ApaLI digests > mCherry has ApaLI site* (will cut within RFP-Repressor genes, see 6/04/11)

--> Pick and prep 2 new 192/196's (#9) (tomorrow) --> For all others, inserts appear to be correct, but there should be a total of 7 bands for all constructs. Confirm via sequencing (tomorrow).


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