BCH112106-construction of pJ23006-J23098, 99, 122, 123, 124, 125

[j23085] + [BamHI/HindIII insert which changes key binding frame] j23098
 * PCR Extend bh005F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
 * Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
 * Product is pJ23006-J23098
 * Screen by digesting product AlwNI/XhoI

j23099
 * PCR Extend bh006F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
 * Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
 * Product is pJ23006-J23099
 * Screen by digesting product AlwNI/XhoI

j23122
 * PCR Extend bh007F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
 * Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
 * Product is pJ23006-J23122
 * Screen by digesting product AlwNI/XhoI

j23123
 * PCR Extend bh008F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
 * Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
 * Product is pJ23006-J23123
 * Screen by digesting product AlwNI/XhoI

j23124
 * PCR Extend bh009F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
 * Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
 * Product is pJ23006-J23124
 * Screen by digesting product AlwNI/XhoI

j23125
 * PCR Extend bh010F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
 * Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
 * Product is pJ23006-J23125
 * Screen by digesting product AlwNI/XhoI