User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/01/26

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Anti-sa
 * Somewhat successful. High levels of background (or expression) in muscle sheath. However, appears to be expressed in hub and cells surrounding/touching the hub.
 * Anti-bam
 * Successful. Moderate levels of background, but easily able to differentiate background from signal. Expressed in spermatocytes prior to differentiation.
 * Topro-3
 * Successful. Background is gone.
 * PCR
 * Ran 1% gel at 105v for ~100 minutes
 * All samples produced a 700bp band, but is working. Potential problem with negative control?


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