IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/03/Zhangs

Add CIAP into 12 RBS digesting vector samples. Identify the digested ligation product of CI+1-23L/1-4H and RBS vector by electrophoresis System:

Marker:

Digestions for RBS vectors failed and we digested again. Samples: 1-11N, 1-1H, 1004-4C, 1-2M, 1-2I, 1-5N, 1-2G, 1-2K, 1-5J, 1004-3C, 1004-2G, 1-1J System

Gel Extraction for CI+1-23L/1-4H insert Gel: CI+1-23L 0.139gPN volume: 417μL CI+1-4H  0.071gPN volume:213μL

Identify the RBS digestion products by electrophoresis Results (from left to right) Plasmid control, 1-2I, 1004-2G, 1004-4C, 1-5N, 1004-3C, 1-2K, 1-1H, 1-1J, 1-2G, 1-11N, 1-2M, 1-5J There are no strips for thee 2008 iGEM parts 1004-2G, 1004-4C, 1004-3C. Perhaps the quality of the 2008 parts is bad.

DNA purification to extract RBS vector

Ligate the RBS vector and CI+Terminator insert System