User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/16

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Wednesday 16 June 2010
I performed a double restriction of the cI inverter plasmid with SpeI and PstI as follows:

Reactive...........1x [ul]

DNA................10

BSA..................1

Buffer2.............3

H2O................13

SpeI.................1.5

PstI..................1.5

Total................30

The digestion was left in the incubator at 37º C for 4 hours.


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