IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/16/Zhangs

Do the PCR identification of 1-2M/11N/5J/1J/5N/2K/2I/1H/2G+T7ptag+T using bacteria liquid; 45 samples in total, 5 samples for each RBS.

Electrophoresis to identify the 9 RBS+T7ptag+1-23L PCR product Result 1 (from left to right, from top to bottom): sample loading order Up: 1-2K+T7ptag①~⑤, 1-2M+T7ptag①~⑤, 1-1J+T7ptag①~⑤, 1-2I+T7ptag①~②, Marker Down: 1-2I+T7ptag③~⑤, 1-5N+T7ptag①~⑤, 1-5J+T7ptag①~⑤, 1-11N+T7ptag①~④, Marker The 1-2K+T7ptag①~⑤, 1-2M+T7ptag④, 1-1J+T7ptag①~⑤, 1-2I+T7ptag②~④, 1-5N+T7ptag①~⑤, 1-5J+T7ptag①,⑤, 1-11N+T7ptag①, ③, ④, ⑤ are target plasmids. Result 2 (from left to right) 1-11N+T7ptag⑤, 1-2G+T7ptag①~⑤, 1-1H+T7ptag①~⑤, (previous RBS+CI product), Marker The 1-2G+T7ptag①~⑤, 1-1H+T7ptag①, ④, ⑤ are target plasmids. Store the spawns of 1-2K+T7ptag3, 5 1-5N+T7ptag3 1-2M+T7ptag4 1-5J+T7ptag1 1-1J+T7ptag5 1-11N+T7ptag3 1-2I+T7ptag4 1-2G+T7ptag1 1-1H+T7ptag4 10 samples in total, sent to be sequenced.