IGEM:Peking University/2008/Experiment/PKU 0807019 G1 1

PKU_080719_G1_1

 * Transformation-single/double ligation-pGREG504-pAID-lac2/Control(C1~C5)


 * Gel running-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)


 * Gel extraction-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)


 * Changing kit (QIAquick)


 * Gel running-check extraction product


 * lanes:1BS|SBS|TBS|AII|PGSX|1SX|SSX|TSX


 * Single Ligation-pGREG504-pAID-lac1/S/tet


 * Double Ligation-pGREG504-pAID-lac1/S/tet-AID


 * Control C2-1


 * Control C2-2


 * Control C2-3


 * Control C2-4