User:Karmella Haynes/Notebook/BioBrick cloning/2009/11/25

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11/25/09

 * &#x2713; Minipreps: KAH94(4), 95(4)
 * &#x2713; Cultures: KAH96(2), 97(2), 98(2), 011(6)
 * &#x2713; Make 10x P1 buffer
 * &#x2713; Check KAH011 & 012 sequences

Minipreps > Check with E/P digests

Buffer P1 (Qiagen) > 50 mM Tris·Cl pH 8, 10 mM EDTA, 10 μg/mL RNAse A > 10x stock P1: > Autoclave > To use, dilute 10x to 1x with sterile dH2O; add RNAse A to 10 μg/mL; store at 4°C

Sequence results
 * 1) KAH011-1-P0001: bad clone
 * 2) KAH011-2-P0001: bad clone
 * 3) KAH012-1-P0001: good (keep this one)
 * 4) KAH012-2-P0001: good

> Pick 6 clones from KAH011 plate for new minipreps, then sequence


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