WangLab:Cell Culture Protocols

Notes About Avoiding Contamination
All the cell culture should be performed in the cell culture hood. And please pay special attention to avoid contamination. Here are a few examples to follow.
 * 1) Clean up the cell culture hood using ethanol before and after experiment.
 * 2) If you are moving any bottles from outside into the cell culture hood, please be sure to wipe it up with ethanol first.
 * 3) Discard the pipet tip if it touches anything unsterilized in the hood, to avoid baterial growth in the cells.
 * 4) Only use clean pipet tip to get cell culture medium; keep it away from cell culture dish to avoid passing cells in to the medium stock.
 * 5) If you switch cell type, please be sure to switch to a new vacuum tip to avoid cross contamination of different cell types.

Protocols
Passing Cells

Freezing Cells

Thawing Cells

Transfection (Lipofectamine 2000)