Template:SBB-Protocols Enz7

EcoRI/BamHI Digest of vector plasmid
3uL of vector DNA 3uL of NEB Buffer 2 1.5uL EcoRI 1.5uL BamHI 21ul of ddH2O
 * Set up the following reaction:
 * Incubate at 37 degrees on the thermocycler for 1hr
 * Run an agarose gel, and melt with 600uL ADB buffer at 55 degrees. ****NOTE:  If you are running short of time, this is an acceptable stopping point