IGEM:IMPERIAL/2007/Notebook/2007-8-23

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/23 view=threemonths format=%name/%year-%month-%day weekstart=7

Preparation of Reaction Buffer for S30 Cell Extract

 * 1) Calculated and weighed out required amounts of reagents
 * 2) Left mixture on the stirrer in the cold room overnight
 * 3) *Phosphoenolpyruvate to be added tomorrow

Protocols can be found at S30/S12 Cell Extract in the general protocols page

Vesicles Formation with GFP
Preparations

The desiccation and suspension stages of the vesicle preparation protocol were followed.
 * One 100ml beaker was prepared with 0.5mg of powdered POPC in 50ml of dodecane.
 * The suspension was sonicated for 30mins and left overnight in a 27&deg;C incubator.