Template:SBB09 19395

Your Name

Invasin (native, short)
Source: plasmid pAC-TetInvdBg You should read PMID: 11137298 for some background. You will be constructing parts derived from the inv gene of Yersinia pseudotuburculosis. The source for your parts is a plasmid, and the sequence is [[Media:pAC-TetInvdBg.seq | here]]. You are going to construct two parts derived from Invasin. Your forward oligo will be reused in the two construction files. For the first part, the short version, you should make an "a~" part that begins from the native start (start of red region in the plasmid map file) and extends up to the lime green region (but doesn't include any of the lime green sequence).

Due to the large size of this part, you will not sequence every base of your plasmid. You should only sequence with ca998 and G00101 (forward and reverse) and confirm the sequence of the 5' and 3' 500 bp or so.

Invasin (native, long)
Source: plasmid pAC-TetInv Your second part is much like the first, but you will include the lime green region in the part.

Due to the large size of this part, you will not sequence every base of your plasmid. You should only sequence with ca998 and G00101 (forward and reverse) and confirm the sequence of the 5' and 3' 500 bp or so.

Note: The reverse oligo for this part is the same oligo being used for the invasin part in project SBB09_41163. You should coordinate with the person who got that project in designing a single oligo for both parts.