IGEM:MIT/2007/Notebook/2007-6-15

Grad Advisors

 * Morning: Debbie, Forrest
 * Afternoon: Brian

LAB WORK
Previous night:
 * Analyze plates
 * innoculate 4ml LB/Amp

Day of:
 * Mini-prep plasmid DNA

Results from last night's incubation
Contents   Volume    Label    # of Colonies U V        (2 µL)    I        67 ddV        (5 µL)    A        0 ddV + ddI  (5 µL)    B        0 dV         (5 µL)    C        28 dV + dI    (5 µL)    D        49 ddV        (10 µL)   E        0 ddV + ddI  (10 µL)   F        5 dV         (10 µL)   G        0 dV + dI    (10 µL)   H        5
 * All plates with colonies have feeder colonies

Plan

 * 1) Plate and look at them.
 * 2) At least a couple colonies.
 * 3) if lots, pick 2 colonies from each; doesn't matter 5 ul or 10 ul. Just need 4 different types
 * 4) One colony into one tube, and repeat. So we have 11 innoculation tubes; 2 for each type and all 5 for ddV/ddI.
 * 5) Put LB amp into glass tubes with metal caps (flamed)
 * 6) Loop and insert colonies.
 * 7) Place plates in fridge
 * 8) Place tubes in 37°C room overnight
 * 9) Store in refigerator for mini-prep on monday

Tubes:
 * 2: U V
 * 2: dV
 * 2: dV + dI
 * 5: ddV + ddI