User:Karmella Haynes/Notebook/BioBrick cloning/2011/01/13

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01/13/11

 * &#x2713; Minipreps: KAH171(2), KAH172(2), KAH173(6), MI127(2)
 * &#x2713; Sequencing order: KAH171, 172

Minipreps > Check KAH173 mp's with E/P digests

--> Conclusion: Not confident about these, especially since the ratio was ~1:2. Re-do cloning. Dephos. vector and use more insert.

Sequencing (Genewiz, standard)
 * 1) KAH171-1, P0001: good (keep)
 * 2) KAH171-2, P0001: good
 * 3) KAH172-1, P0001: good (keep)
 * 4) KAH172-2, P0001: good


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