IGEM:MIT/2006/Notebook/2006-8-30

to do

 * 1) digest J45298 miniprep (from 8/29) with XP (run a gel to make sure it looks correctly mutagenized) -done
 * 2) glycerol-done/miniprep -done/sequence J45396 -done
 * 3) glycerol new Q119 -done
 * 4) sequence osmY (SO and SN) constructs in bright green box b/c smell test data is not on wiki -done
 * 5) resequence 0-Q-199A in case rerun doesn't work -done
 * 6) 3-part assemble rbss to pchBA.15-muts and transform into top10 -done
 * 7) transform O-Q-119 and O-Q-199 (?) into IK to smell test -done
 * 8) pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs -done, but repeated the ligation/transformation due to 2XYT contamination
 * 9) LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block) -done
 * 10) smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -done
 * 11) set up a new pseudomonas test -done