User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/05

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Bradford & cAMP precipitation
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Bradford (Enspire 2300 & Tecan Safire)
 * cAMP precipitation

Results

 * Sadly no difference could be seen between Enspire and the Tecan reader, and the RIPA buffer (not lysis), still yielded a very high signal
 * Everything was put equal to sample S1 and 1 mL of put to 30 μL of 8-AHA-cAMP agarose beads (3x)

Discussion

 * Maybe Pierce assay might be a better alternative to the Bradford assay is something in the buffer is interfering

Attachment
   [[Media:Bradford_05072010.xls|Bradford results both Tecan and Enspire]]


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