Griffitts:Nodule Recovery

Procedure

 * Place plant in a puddle of water
 * The lid of a Petri dish works nicely
 * Cut off the nodule using a sterile razor blade
 * Place nodule in a microcentrifuge tube with 500 μL dH2O
 * Be sure to label your tubes
 * Decant dH2O
 * Add 500 μL 75% ethanol
 * Wait 30 seconds
 * Decant ethanol
 * Add 500 μL 50% bleach
 * Wait 60 seconds
 * Decant bleach
 * Wash 2–3 times with sterile dH2O
 * Add 200 μL LB-10% glycerol
 * Macerate the nodule with a blue pestle
 * Pipette 5 μL to the appropriate selective medium and streak out with a single toothpick
 * This should be ~100 CFU
 * Be sure to label your plate
 * Freeze down the remaining solution in the –80°C freezer