IGEM:IMPERIAL/2006/LabCalendar/2006-7-26

For Maxiprep
 * F2620 well 6B
 * C0261 well 24A
 * I13504 well 12D

Transferred to 2mL LB liquid culture in preparation for Maxiprep - in 37C shaker

For Miniprep
 * I13033 well 2H
 * J04500 well 16P
 * C0060 well 5M

Above transfereed to 2mL LB liquid culture in preparation for miniprep - in 37C shaker


 * R0062 well 9G
 * B0034 well 3O
 * C0062 well 7A

Above transferred to 2 mL LB liquid culture in preparation for miniprep. Separately, from previous miniprep, took 10 uL out to digest and to run gels later..
 * Made up 1% agarose solution, 60 mL ready for 3 gel plates, 2 mL ethidium bromide, and set to cool
 * Added TAE to prevent from drying out
 * A/O 12pm, the three digests are in 37C water bath before moving to gels in an hour

Prepare the following parts for Maxiprep and ligations for tomorrow
 * I13033 well 2H
 * C0062 well 7A
 * F2620 well 6B
 * C0261 well 24A
 * R0062 well 9G
 * B0034 well 3O
 * I13504 well 12D

Culture 100 mL LB and 200 uL bacteria with part in 500 mL flask. Set overnight in 37C shaker to culture.