Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-10

James Field: Aims:

Morning: 1) Finish extracting BioBricks from Registry.
 * BBa_R0011
 * BBa_K118011
 * BBa_I0500

2) Train up a Bioengineer and begin promoter testing.

Results:

AM:

Made a start on colanic acid wet lab prep. Deadline for completion set to Wednesday.

PM:

Extracted BioBricks from registry as planned.

--David Roche 12:14, 10 August 2009 (EDT)  Achievements  Focused on trehalose section of Module 2: Discussed with James and Nuri in the morning the design rationale of Module 2. Came up with a list of criteria to be filled in for each section - trehalose. Worked on developing trehalose assay

Royah Vaezi 17:28, 9 August 2009 (EDT): Aims:

1)Finish extracting BioBricks from Registry with James/show bioengineers.
 * BBa_R0011
 * BBa_K118011
 * BBa_I0500

2)Train up a bioengineer and begin PCR for cloning & primer design for SLIC.

Aims:
 * Charles Fracchia 05:08, 10 August 2009 (EDT):

1) List all jobs and assign them

2) Decide on website architecture and implement foundations
 * Decide on technologies used:
 * Flash
 * Javascript
 * Create browser-selective script
 * Create Flash to Javascript messages/switch

Aims:
 * Charles Fracchia 05:08, 10 August 2009 (EDT):

1) MATLAB tutorial 2) Timer design: DRYLAB only?
 * 1h or 2
 * James: Implement Glucose/Arabinose in the lab as well as model it
 * Kun: No wetlab implementation
 * Dave:
 * Nuri: not clear, not spend too much
 * TianYi: timer is not essential, focus more on encapsulation
 * Dineka: give priority encapsulation


 * Characterise the outputs of experiments
 * Aim for step by step approach
 * Journal Club: Nuri send paper round
 * Summarise the set of data that we are getting from experiments, with testing constructs and everything

Aims
 * Nuri Purswani 09:49, 10 August 2009 (EDT):
 * Help out with planning constructs for M2 (relationship between [glucose] and CRP.
 * Modelling considerations: Dry lab plan

Weekly aims:
 * Organize wiki
 * Send list of people and availability through september Done but needs discussion

Updated weekly aims
Split up into modules and fill in the following criteria: DNA, assembly, assays, output data from experiments, shopping list for each protocol, modelling perspectives. Module navigation

Module 1
DNA constructs  Done Assembly  Needs to be revised for SLIC usage Assays  Done, Protocols transcribed to OWW pages Output Data  Give feedback plz Shopping list  In progress Modelling perspectives  Needs detailing

Module 2
 Split up : Nuri - Promoter Characterisation James - Colanic Acid Dave - Trehalose Production 

DNA constructs

JF Done Please Feedback DR Done Please Feedback

Assembly

DR : Not yet done,

Not Done - JF

Assays

DR : In Progress,

JF in progress - Please Feedback

Output Data DR : In Progress (feedback) JF in progress - Please Feedback

Shopping list DR : Done (feedback) JF Done Please Feedback

Modelling perspectives DR : done In Progress, JF in progress - Please Feedback

Module 3
 Split up : Promoter Thermoinduction - Kun Killing - Dineka Methylation 

DNA constructs

KX Done Please Feedback DK Done Please Feedback

Assembly

KX & DK: Not yet done.

Assays KX : Done - Beta gal & temperature shift. DK : Done - Staining. CFU in progress.

Output Data KX & DK : In Progress

Shopping list KX : Done - Beta gal & temperature shift. DK : Done - Staining. CFU in progress.

Modelling perspectives KX & DK : In progress.