Chang Lab:Notebook/CBE/08/149/2008/12/10

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 * style="background-color: #FFF"|   Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
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 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page


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Logs

 * Repeat inoculum preparation as in 12/9.
 * This time only using 1 loop instead of the 5 loops for 150ml broth. Reason: to reduce the number of colonies on the agar petri dish so that counting would be more manageable.


 * Absorbance reading started at 530pm.
 * Number of time points reduced to 0th hr, 3-5th hr, 14th, 16th and 24hr.
 * Serial dilution was increased 10^-6, 10^-8 and 10^-10 for (1st 8-hr incubation; while plate 10^-8, 10^-10, and 10^-12 for subsequent hours.


 * Note
 * Growth curve of 2nd mid log phase is similar to that of the 1st. Mid log phase of E coli is 5-6th hr.


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