Annealing complementary primers

See also Annealing primers

Introduction
A simple and cheap way to make a short (< 100 bp) piece of DNA is to order two complementary primers from a company such as Invitrogen. Other options are described under part fabrication and annealing and primer extension. Having annealed the primers, you most likely want to use them as an insert into a vector (the linked page describes BioBrick vectors). Here are some pages that describe methods to assemble two parts if they correspond to a BioBricks standard -
 * 1) BioBricks construction tutorial
 * 2) 3A assembly
 * 3) Silver lab strategy.

Lab Protocols
Endy:Annealing complementary primers Silver:Annealing complementary primers Koch Lab (For single-molecule tethering purposes.)