IGEM:Yale/2010/Protocols/Transformation

=Standard Transformation Protocol=


 * 1) Preheat SOC broth at 37 °C
 * 2) Thaw competent cells on ice
 * 3) Aliquot 50-100 μL of cells into two pre-chilled 15 mL culture tubes (one for the control, one for the actual transformation)
 * 4) Add 1-2 μL of plasmid and swirl gently
 * 5) Incubate tubes on ice for 30 minutes
 * 6) Heat pulse tubes in 37°C degree water fro 30-40 seconds
 * 7) Incubate tubes on ice for 2 minutes
 * 8) Add 500 μL of preheated SOC broth to each tube and incubate for an hour at 37°C with shaking
 * 9) Spread 100 μL of each culture on a LB agar plate containing the appropriate antibiotic(s) and incubate overnight at 37°C