IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/29

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6/29/10
 5:30PM 

Digestion of backbones

Samples: pSB1A2, pSB1C3, pSB1T3, pSB2K3 (BBa_E0033), pSB1AC3, pSB1AK3, pSB1AT3 Incubate at 37 &#186; C, 15 min

Inactivate at 80 &#186; C, 20 min

PCR cleanup

add 2 ul SAP buffer, 1 ul SAP

Incubate 37 &#186; C, 10 min

Incubate 65 &#186; C, 15 min

Gel

175 V, 1% TAE


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