IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/06/28

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June 28, 2010

 * Met with the Synthetic Biology Research Group
 * Measured fluorescence of YFP and RFP with the spectroflourometer using filters
 * Measured the fluorescence of CFP with the plate reader
 * Plated CRP, RFP, YFP
 * Made TB liquid cultures of CFP and YFP
 * Used the spectrophotometer to check the yield of the previously made CFP (1.5 ml and 1.0 ml)

CFP-1.5-A	 30.0 ng/ul CFP-1.5-B	 48.0 ng/ul CFP-1.0-A	 21.4 ng/ul CFP-1.0-B	 23.9 ng/ul


 * Performed the PCR of the copper promoter and fluorescent protein cassette- performed first PCR (added adapter primers to CFP) and performed the second PCR (extended to full copper promoter and fluorescent protein cassette)
 * Autoclaved assorted materials and bio hazard waste
 * Made LB antibiotic agar


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