Michael Eisen Lab:Reagents:PBS

Phosphate Buffered Saline

Usage
PBS is an isotonic buffer for cells.

Purchase in Solution
This should be used as a last resort.


 * PBS is available as 10X Solution from the Stanley Hall stock room

Prepare from tablets

 * PBS is ideally prepared from PBS tablets or constituent salts (see wikipedia page).
 * Sterilize solution with sterile filter.

1X PBS
1x PBS is 137 mM NaCl, 12 mM Phosphate, 2.7 mM KCl, pH 7.4

10X PBS Stock Solution

 * 1) Combine the following:
 * 2) * 80 g NaCl
 * 3) * 2 g KCl
 * 4) * 14.4 g Na2HPO4 (dibasic anhydrous) OR 18.1 g Na2HPO4 · 2H2O (dibasic dihydrate) OR 27.2 g Na2HPO4 · 7H2O (dibasic heptahydrate)
 * 5) * 2.4 g KH2PO4 (monobasic anhydrous)
 * 6) * 800 mL distilled H2O
 * 7) Adjust pH to 7.4 with HCl
 * 8) Add H2O to 1L
 * 9) Autoclave for 20 minutes on liquid cycle or sterile filter.  Store at RT.

Storage

 * PBS tablets on chemical shelf
 * PBS stock solutions on benchtops

Wikipedia.org PBS Page