IGEM:IMPERIAL/2007/Calendar/2007-8-29

We are in Week 8  of the iGEM project

Project Calendar
name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7

Infector Detector
Comments
 * This application is a big leap forward for the medical field.
 * If we perfect this application, we will be able to put it on every catheter.
 * Think about the packaging of the system
 * Does the in vitro system really give us the edge over the applications in the field of synthetic biology?
 * How do we add LuxR (packaging)
 * For the Jamboree presentation, take the audience through the research and application process step by step to make it easily digstible

In the lab
 * Carry out this experiment in vivo and observe the edge it has over the in vitro application
 * Is the DNA construc complicated enough?
 * Define visible- DsRed Express and GFP; change the conditions ofobservation of the fluorescence produced
 * How sensitive is the system?
 * How long can the system bestored at e.g.4oC before usage?
 * Problem with detection of low [AHL]
 * How long can the system last? Can we optimize it?
 * How stable is the fluorescent molecules?
 * How robust is the system? e.g. temperature dependance, pressure dependant

Cell By Date
Comments
 * Clarify experimental concept, and link it to meat spoilage
 * Emphasize of DsRed Express as the reporter
 * Think about the packaging of the product e.g. sticker form? in the cling film?
 * Might have to look for another application other than meat spoilage, if construct does not fit the temperature range well

In the lab
 * Place the device in a real scenario and observe how it works
 * Find out the lag time of the system i.e. how long would DsRed Express take to reach visible threshold
 * Construct does notfit the temperature range very well
 * Look into cryophilic bacteria, which has the appropriate cellular machinery to generate proteins at the temperature range of 0oC -5oC
 * Look at improving the sensitivity (lag time) of the system
 * Look into making new construct for pT7 as a biobrick from the current version we have so that we can swap bricks around later

Vesicles
Comments
 * It is a new chassis
 * If the pores used are toxic, should they be placed on the catheter?
 * For the presentation, link this with the 2 applications
 * Want to see the engineering cycle implemented into our project
 * It is not essential to the main project, but serves as "icing on the cake"
 * Less manpower should be devoted to it (reduce from 3 members)

In the lab
 * It is very hard to calibrate fluoresence withinthe vesicle; if solution used is differet, vesicles would also behave differently
 * It serves to increase the lifespan of the CBD application, but a strict control must be imposed on the quality

PR

 * How do the 2 projects (applications) link together?
 * Implement the engineerng cycle
 * Sherlock Holmes theme is a good selling idea