Template:SBB09 18094

Hank Shih

Intimin (native)
Source: E. coli strain O157:H7 For background, you should read PMID: 11717287. You will be making a part derived from the eaeA gene which encodes the Intimin protein. You can download an annotated sequence [[Media:eaeA_region.seq | here]]. You will be making an "a~" style part out of the green and pink regions of this file. Note: You will be sharing the reverse oligo with whoever gets the SBB09_19607 project. You should find this person and design that oligo together.

Fast-Degrading GFP
Source: pBca1256-Bjh1857    {} This part has already been constructed as a BglBricks basic part in plasmid pBca1256. You will be transferring the part using the Gateway reaction into the assembly vector. To do this, you will react the source plasmid with plasmid pBca1254AK. As background, you should read PMID: 11059491.



This part encodes a passenger protein
After performing the Gateway reaction, your product plasmid will be pBca9495AK-partname. To see what the part looks like in the product vector, look at [[Media:JCA_pBca9495AK-Bca1144.seq | this map]] of the RFP-expressing part Bca1144 in pBca9495AK.