SBB09 Construct21

Wobble Osc0028F/Osc0029R          (136bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5   (EcoRI/BamHI, 3171+910, L) Product is pBca9495AK-M10053     {passenger protein}

Osc0028F  Forward construction of passenger protein basic part TCCGGgaattcATTagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG Osc0029R  Reverse construction of passenger protein basic part CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG

JCA: Put atg between eco and bam; renamed short description. Revised below:

Wobble Osc0028F/Osc0029R          (136bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5     (EcoRI/BamHI, 3171+910, L) Product is pBca9495AK-M10053    {}

Osc0028F  Forward construction of Leucine zipper EILD TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG Osc0029R  Reverse construction of Leucine zipper EILD CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG