BioMicroCenter:Genome Seq

Genome Seq
De novo sequencing can be done using the Genome Analyzer. Read lengths have recently been increased and data associated with these long reads look very good! http://illumina.com/downloads/ch2-ILMN_ProdGuide_DNAsequencing.pdf

Sample Submission Guidelines
Users are required to prepare their own libraries for sequencing by following the corresponding Illumina Protocols. If this is a service that you would like the BMC to perform in the future please visit the Ideas page. Illumina Paired End sample preparation kits can be purchased directly from Illumina or through the BioMicro Center for $3,600.00 plus shipping costs (10 sample preps per kit). To request an order or for more information please contact Ali Perrotta

Samples which are ready for sequencing should be gel-purified according to your protocol, and provided as at least 5uL of solution at a concentration of at least 10 nM in EB/ Tween 0.1%. Quality control will be run on all submitted samples.

Samples generated using standard kits with Illumina PCR primers can be submitted without sequencing primers. Custom sample preparations using customized PCR primer sequences should be provided along with a complementary sequencing primer.

Sample Submission Forms

Data
Images acquired from the solexa sequencer are processed through the bundled solexa inage extraction pipeline to get the sequence and quality score for each base. The data is aligned to a reference genome, if requested, using an interative ELAND algorithm. An in-depth QC report is included in the package. .

Genome Seq Literature

 * 1) Paper1 pmid=18677319