User:Kristina M Holton/Ovarian Notebook 2007 9 14

Qiagen RNeasy FFPE Kit: G02, G03, Drapkin

10-10 micron samples, split into 2 tubes (50 microns each)


 * Step 5: Well-pelleted, no visible tumor sample in G03
 * Step 7: White & cloudy
 * Step 9: Better pelleted
 * Step 11: Cloudy, using larger volumes
 * Step 12: After 80C, clarified
 * Step 15: 1750 EtOH for G02 as per Supplemental Protocol (too much for tube), 1400 EtOH for G03, Drapkin (fits into tube, original protocol calls for 1300ul), Small amount of precipitate
 * Step 16: G02 requires 4 passes through column
 * Step 21: Eluted in 30ul