IGEM:metu/2009/Notebook/wound dressing/2009/10/07

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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07.10.2009
1. We prepared LB Broth. (190 ml,250 ml, 250 ml and 250 ml) They were named as Lard1+ABC (1), Lard1+ABC (2), Lard1 (1) and Lard1 (2).

In Lard1+ABC (1) ; 190 μl Ampicillin, 646 μl chloramphenicol and 200 μl bacteria with Lard1+ ABC were add. In Lard1+ABC (2) ; 250 μl Ampicillin, 850 μl chloramphenicol and 200 μl bacteria with Lard1+ ABC were add.

In Lard1 (1) ; 250 μl Ampicillin and 200 μl bacteria with Lard1 were add. In Lard1 (2) ; 250 μl Ampicillin and 200 μl bacteria with Lard1 were add. We put these flasks into shaker, 37 C, 190 rpm.