Template:SBB10Project-20154

Maziar Behtash

sbb19: FokI Cleavage Domain (fok+)
Source: Registry part BBa_K243000 in vector BBa_K157000 Target Sequence: CAGCTGGTTaaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaacctgatggtgccatttataccgttggttccccgatcgattatggcgttatcgttgataccaaagcctatagcgggggttataacctgccaattggtcaggctgatgagatgcagcgttatgtgaaagagaaccagactcgtaacaaacacatcaacccgaacgaatggtggaaagtgtatccgtcaagcgttacagagttcaaattcctgttcgtgagcggccattttaaaggcaactataaagcacagctgacccgtctgaaccataaaaccaatTgcaatggcgccgttctgtcagtagaagagctgctgattggcggtgaaatgatcaaagccgggaccctgacactggaagaagttcgccgtaaattcaacaatggggagatcaattttTAA Vector: pBjk2741 Short description: <FokI+! Flavor: {<part!} Family: Restriction Endonuclease Your part exists in the Registry of standard biological parts, but this template sequence differs from what you want by 1 mutation and 3 extra AA at N-term. Take careful note in designing your oligos that you generate the correct product.

sbb23: Zinc Finger target
Source: Synthetic Target Sequence: TGTTCGGAGCCGCTTTAACCCACTCTGTGGAAGTGCT Vector: pBca9145 Short description: ZFNS target Family: Restriction Site