IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/23/Zhangs

Double digestion for Terminator vector Digestion system: After 90min digestion, add: 1~2μL CIAP+5μL CIAP buffer

Ligate the CI insert and Terminator vector.

Transforming the CI insert + Terminator vector ligation product.