IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/30/Zhangs

Double digestion of CI System for front insert: 4 h digested Electrophoresis Result (from left to right) CI, CI plasmid control, marker Gel: 0.072gPN volume: 216μL Gel extraction for CI insert

Ligation  Insert:CI  Vector:1-23L, 1-4H System (1)7:1 system

(2)3:1 system

Control 6 samples in total, 2 for 1-23L, 2 for 1-4H, 2 for 1-23L & 1-4H controls