IGEM:MIT/2005/Beginning of Week Meeting: Monday, July 5

Top Priority (can be done today)

 * 1) Figuring out fusion point - where to attach ToxR to scFvs. Need help reading paper.
 * 2) *Why was this place chosen on other papers? Use this to help figure this.
 * 3) *What else did they try? What didn't work, why?
 * 4) *Look at references from article.
 * 5) Specify this week -> next week's experiments.
 * 6) Controls, what to expect
 * 7) Getting cells from Harvard - learning about them, reading papers.
 * 8) Order Biobricks
 * 9) *In parallel: order primers to make BioBricks in lab.
 * 10) *scFvs 1-3
 * 11) *ToxR

To Do This Week

 * How to get library of fluorecein dimers
 * Get ToxR and ToxR-cI plasmids from Harvard

Converting to BioBrickism

 * Get rid of incorrect restriction sites, attach biobrick ends.
 * See codon usage to ensure efficiency in transcription. (Take out rare codons)
 * Biobrick domains. Allow for exchange of scFv easily.

Issues

 * Protein domains - LD linker is fixed.