IGEM:Melbourne/2008/Notebook/BioClock/2008/08/05

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] Melbourne iGEM 2008 -- BioClock
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Done today:

 * Red light retest.

4 transformations:
 * Cph8 + pPL-PCB
 * Cph8
 * PCB
 * -ve control

Protocol:
 * 2μL DNA
 * RU1012
 * 30 min ice
 * 1 min 42°C
 * 1 hour at 37°C + LB

Each transformation plated onto 2 plates, one of which is wrapped in foil. Placed in 37°C room at genetics which light on

Plates: Amp Kan Chlor Arabinose (2mM) IPTG (1mM) X-gal

Alex


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