User:Morgan L. Paull/Notebook/Genetic Fence/2011/01/20

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Checking Act2LacO and parts which contain it

 * Cut 10 parts, either Act2LacO or parts which contain Act2LacO, with Eco and Pst in 10μL reactions
 * Each gel image is separated by more time running on gel - the first after 15, the second after another 15, the final after another 30 minutes




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Gel Extraction

 * Cut out the proper length band (see final gel image)
 * Performed gel extraction in preparation to ligate the Act2LacO promoter into a V0120 backbone

Team Fence Boxes Clean-up

 * Culled 5 team fence boxes from the -20°C freezer, consolidating into just three boxes - hopefully at some point only 2.

Transformations

 * Transformed minipreps from several fence parts to make glycerol stocks of them