IGEM:Brown/2008/Notebook/Team Resistance/2008/06/11

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Team Resistance

 * Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution.

Testing apparatus with NaCl at different concentrations

•	3 plates with 0.05M NaCl (0.02922g in 10mL)

•	3 plates with 0.005M NaCl (0.02922g in 100mL)

•	3 plates with 0.001M NaCl (0.01461g in 250mL)

dilute to each concentration then put 10 mL of each solution in respective plates

Control: distilled water

Results:

Control: 4.108Mohm, 2.517Mohm, 2.934Mohm

0.001M NaCl: 393.7 kohm, 265.5kohm, 579.6kohm

0.005M NaCl: 264.9kohm, 266kohm, 326.1kohm

0.05M NaCl: 233.5kohm, 46.69kohm, 97.1kohm

control	0.001M NaCl	0.005M NaCl	0.05M NaCl

3.186MΩ	0.4129MΩ	0.2857MΩ	0.1258MΩ

0.001M NaCl: 2.7731MΩ change

0.005M NaCl: 2.9003MΩ change

0.05M NaCl: 3.0602MΩ change

•	Our wires were not long enough to touch the solution in the plates—next time don’t tape wires in place until right before experiment begins

•	WE NEED A COMPUTER BASED VOLTMETER

Questions for Dan Ludwig (Meeting Friday 3:00pm)

Should touching the plastic significantly change the resistance?

What if distance changes slightly?

All considerations of our apparatus

Any suggestions for a real-time, computer based voltmeter?

Tomorrow: get plates from Harvard lab!

Create glycerol stocks of the plasmids


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