User:Michael Verhoeven/Notebook/iGEM 2009 M. Verhoeven Parts/2009/07/09

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Labwork
Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation


 * BBa_J23109 (labelled as no.1) vector BBa_J61002 in E.coli TOP10
 * BBa_J23100 (labelled as no.2) vector BBa_J61002 in E.coli TOP10 (red colony)
 * BBa_J23106 (labelled as no.3) vector BBa_J61002 in E.coli TOP10 (red colony)
 * The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul).
 * The tubes were put in a waterbath at 37C (200 rpm) and grown o.n.