Template:SBB10Project-18094

Jing Luo

sbb20: FokI Cleavage Domain (fok-)
Source: Registry part BBa_K243001 in vector BBa_K157000 Target Sequence: CAGCTGGTTaaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaaccagACggtgccatttataccgttggttccccgatcgattatggcgttatcgtggAcacaaaagcgtattctggcggttataatctgccgattggtcaggctgatgagatggaacgttatgtggaagagaatcagacccgtaacaaacatctgaacccgaacgaatggtggaaagtgtatccgtcaagtgtcaccgagttcaaatttctgttcgtgagcggccactttaaaggcaactataaagcccagctgactcgtctgaaccatatcaccaatTgcaatggggcagtgctgagtgttgaggaactgctgatcggtggagaaatgatcaaagcaggcaccctgactctggaagaagttcgccgtaaattcaacaatggcgagatcaattttTAA Vector: pBjk2741 Short description: <FokI-! Flavor: {<part!} Family: Restriction Endonuclease Your part exists in the Registry of standard biological parts, but this template sequence differs from what you want bby 3 mutations and 3 extra AA at N-term. Take careful note in designing your oligos that you generate the correct product.

sbb35: P9 rep Protein
Source: pBca9145-jtk2625 Vector: pBca9523 Short description: {rbs.ColE2 RepA} Family: Replication protein