SEED/2009/Day 7

Morning

 * Discuss sequencing technologies
 * Review labs so far

Afternoon

 * pick 1 blue/green colony per plate for growth and templiphi in strips
 * pick 4 colonies per group
 * 2.5ul sample buffer per tube
 * pick colony and dip in tube
 * 3m@95C to break open cells
 * Add 2.5ul reaction buffer + 0.1ul enzyme mix
 * incubate templiphi at 30C overnight
 * Class project mini-presentations/discussions

Instructor Post-prep

 * heat kill templiphi
 * transfer 2ul of templiphi into sequencing mixes with VF2
 * Send out for sequencing