User:DavidRamos/Lab/2006-6-15

Morning

 * Brainstorming with Pam
 * Conduct ligation reaction
 * 6uL insert, 2uL vector, 2uL buffer, 10uL other buffer
 * 5 min incubation @ RT
 * Conduct transformation
 * 20mL cells for positive, negative, and exp. ea (top 10)

Afternoon

 * Plate transformant cells on LB-CARB
 * Brainstorming session in the afternoon