Template:SBB10 ConstructionFiles CarolynK sbb32

Construction of oriO99 Basic Part sbb32

PCR sbb32F and sbb32R on pEC52          	    (536 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144	           (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb32  		    {oriO99} - sbb32F Forward oligo for cloning of sbb32   ccataGAATTCatgAGATCTagcgcctcagcgcgccgtag sbb32R Reverse oligo for cloning of sbb32   ctgatGGATCCaccaaacgccaacagctcaa



JCA Notes

 * correct