Synthetic complete (SC) medium

Dropout mix

 * A dry powder mix of all amino acids and nucleic acids.
 * Omit the components used for selection
 * Mix end over end with clean glass marbles 15 minutes.


 * Adenine 0.5 g
 * Alanine 2.0 g
 * Arginine 2.0 g
 * Asparagine 2.0 g
 * Aspartic acid 2.0 g
 * Cysteine 2.0 g
 * Glutamine 2.0 g
 * Glutamic acid 2.0 g
 * Glycine 2.0 g
 * Histidine 2.0 g
 * Inositol 2.0 g
 * Isoleucine 2.0 g
 * Leucine 10.0 g
 * Lysine 2.0 g
 * Methionine 2.0 g
 * p-aminobenzoic acid 2.0 g
 * Phenylalanine 2.0 g
 * Proline 2.0 g
 * Serine 2.0 g
 * Threonine 2.0 g
 * Tryptophan 2.0 g
 * Tyrosine 2.0 g
 * Uracil 2.0 g
 * Valine 2.0 g

Total weight 54 g

For SC -ura -trp dropout medium additions to SORB plates

 * Total weight of mix is 50 g
 * For SORB plates with 0.6 g/liter SC dropout medium addition
 * Add 24 mg/liter of Uracil or 24 ml/liter of an 0.1% solution
 * Add 24 mg/liter of Tryptophan or 2.4 ml/liter of a 1% solution

Final SC medium, per liter, autoclave

 * Bacto-yeast nitrogen base w/o amino acids (0.67%) 6.7 g
 * Glucose (2%) 20 g
 * Dropout mix 2.0 g
 * Bacto Agar (2%) 20 g


 * Add 80 mg/liter of Uracil or 80 ml/liter of an 0.1% solution
 * Add 80 mg/liter of Tryptophan or 8 ml/liter of a 1% solution