User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/22

Cellular Adhesion

 * No PCR Supermix until 2pm


 * PCR
 * Template: 40ul PCR Supermix, .8ul primers (.4ul primers for GCN4), .8ul DNA (only GCN4)
 * 6His Tag: 6His_Tag-F and 6His_Tag-R
 * FLAG Tag: FLAG_Tag-F and FLAG_Tag-R
 * Myc Tag: Myc_Tag-F and Myc_Tag-R
 * HA Tag: HA_Tag-F and HA_Tag-R
 * GCN4 Leucine Zipper: GCN4-F and GCN4-R2
 * Same protocol as 1.21.2008