Template:SBB09 41163

Douglas Densmore

Invasin (refactored, long)
Source: plasmid pAC-TetInvdBg You should read PMID: 11137298 for some background. You will be constructing a part derived from the inv gene of Yersinia pseudotuburculosis. The source for your parts is a plasmid, and the sequence is [[Media:pAC-TetInvdBg.seq | here]]. You are going to make the prepro-free version of the part. In the sequence file, the prepo is in navy blue. Your part should include the pink region and the lime green region and not the navy blue region.

Due to the large size of this part, you will not sequence every base of your plasmid. You should only sequence with ca998 and G00101 (forward and reverse) and confirm the sequence of the 5' and 3' 500 bp.

Note: The reverse oligo for this part is the same oligo being used for the invasin part in project SBB09 19395. You should coordinate with the person who got that project in designing a single oligo for both parts.

ChaI Lectin
Source: Plasmid pBca9145-Jtk2031 For background you should read PMID: 15039097. ChaI is a lectin (a sugar-binding protein) that binds to polysialic acid which is the outer shell composition of E. coli expressing the K1 capsule. The 5' end of this part is already correctly encoded in a BglBrick vector. The sequence of it is [[Media:pBca9145-Jtk2031.seq |here]]. The 3' end has a stop codon that you need to remove. So, you'll only need to design one oligo that you can use in addition to this oligo: ca998	Forward Sequencing of pSB1A2/pSB1A3 gtatcacgaggcagaatttcag