McKinney:OADC

Summary
OADC is Oleic Acid Albumin Dextrose Complex, a component of the mycobacterial selective media 7H9 and 7H10. If you don't add the oleic acid solution, then this is ADS, used for M. smegmatis culture in 7H9.

Ingredients

 * deionized water
 * 8.5g NaCl
 * 50g Bovine serum albumin fraction V
 * 20g D-dextrose
 * 50mL 1% sodium oleate solution (for M. tuberculosis only)

Protocol

 * 1) Combine 925mL deonized water and 8.5g NaCl in a 2L flask.  Stir with a magnetic stirbar until salt is dissolved.
 * 2) Add 50g bovine serum albumin fraction V and stir until completely dissolved.
 * 3) Add 20g dextrose, and stir until completely dissolved.
 * 4) Add 50mL 1% sodium oleate solution, and stir until completely mixed.  The pH should now be ~6.9-7.0.  Filter through Whatman #3 paper to remove particulates.  Filter sterilize through 0.22&mu;m pore filter.  Store at 4&deg;C.