Template:SBB-Protocols SmallScaleCompetent

Step 6.5: do the negative control before adding the DNA
 * 1) Grow 30uL of cells in ~4 mL LB until cloudy (OD600=0.5)
 * 2) Put on ice
 * 3) Transfer 1mL into an eppendorf tube on ice, let cool
 * 4) Centrifuge full speed for 30 sec, toss out supernatant
 * 5) Resuspend in 90uL of TSS solution
 * 6) Add 10uL KCM
 * 1) Add 1uL plasmid DNA
 * 2) Let sit on ice for 10min, heat shock 90 sec at 42, ice for a minute, rescue 1 hr, then incubate and/or plate


 * Always do negative controls on your competent cell prep!

Sector a region of your plate and spread about 5uL of untransformed competent cells to confirm that they are free of contamination.