Oneill Lab:Media

Michael J. O'Neill Lab University of Connecticut Department of Molecular and Cell Biology

Recipes for making media commonly used in lab

Bacterial growth media

 * Recipes are for 1L liquid media
 * Can use recipes to make plates by adding bacto agar to concentration of 1.5% w/v
 * Top agar is used by adding bacto agar to 0.7% w/v

LB

 * 1) 10g Tryptone
 * 2) 5g Yeast Extract
 * 3) 10g NaCl
 * 4) Add deionized water to 1L
 * 5) Autoclave

Rich medium

 * 1) 10g Tryptone
 * 2) 5g Yeast Extract
 * 3) 5g NaCl
 * 4) 2g Glucose
 * 5) Add deionized water to 1L
 * 6) Autoclave

SOB

 * 1) 950ml Water
 * 2) 20g Tryptone
 * 3) 5g Yeast Extract
 * 4) 0.5g NaCl
 * 5) Dissolve
 * 6) Add 2.5ml 1M KCl
 * 7) Adjust pH to 7.0
 * 8) Add 10ml 1M MgCl2
 * 9) Autoclave

SOC

 * 1) Add 20ml sterile 1M Glucose to SOB
 * 2) Aliquot and freeze for storage

&psi;b

 * 1) 20g Tryptone
 * 2) 5g Yeast Extract
 * 3) 5g MgSO4
 * 4) Adjust pH to 7.6 with KOH
 * 5) Autoclave

TfbI

 * Recipe for 500ml
 * 1) 1.47g Potassium Acetate
 * 2) 0.61g Rubidium Chloride
 * 3) 0.74g Calcium Chloride
 * 4) 4.95g Manganese Chloride
 * 5) Dissolve salts
 * 6) Add sterile glycerol to 15% (v/v)
 * 7) Adjust pH to 5.8 with 0.2M Acetic Acid
 * 8) *Do not adjust pH with KOH
 * 9) Filter Sterilize

TfbII

 * Recipe for 50ml
 * 1) 10mM MOPS or PIPES
 * 2) *0.15g PIPES
 * 3) 0.55g CaCl2
 * 4) 0.06g RbCl2
 * 5) 7.5ml Glycerol
 * 6) Adjust pH to 6.5 with KOH
 * 7) Filter Sterilize

Links and Contacts
Seth Kasowitz [mailto:seth.kasowitz@uconn.edu]

|O'Neill Lab Homepage