IGEM:IMPERIAL/2007/DNA Constructs/Con Diagrams old

Here lies the ruins of the ancient constructs page


 * Construct CBD 1
 * Entire construct is currently available in Biobricks Registry: BBa_E7104 (available), in pSB1A2 (AmpR)
 * pT7 can be used with T7 RNAP in vitro and in veso, and it gives constitutive expression.


 * Construct CBD 2
 * Entire construct is currently in Biobricks Registry: BBa_I13522 (available), in pSB1A2 (AmpR)
 * pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.


 * Construct CBD 3
 * pBAD promoter with GFP is currently available in Biobricks Registry: BBa_J5528 (available), in pSB2K3 (KanR)
 * Need Ara C regulator protein in vitro and in veso. Ara C available with the RBS:BBa_S03550
 * pBad can be used with E.coli RNAP in vitro, and it is inducible by arabinose.
 * To be used in veso, arabinose transport proteins have to be inserted in the phospholipid bilayer of the vesicles.


 * Construct CBD 4
 * Entire construct is currently available in Biobricks Registry: BBa_J07037 (avaiable), in pSB2K3 (KanR)
 * pcI gives constitutive expression.


 * Construct CBD 5
 * All parts are currently available in the Biobricks Registry.
 * pcI promoter: BBa_R0051 (available), in pSB1A2 (AmpR)
 * Arabinose C regulator protein with RBS: BBa_S03550 (available), in pSB1AC3 (A+CR)
 * Stop codon: BBa_B0015, in pSB1AK3 (A+KR)
 * pBAD with GFP: BBa_J5528, in pSB2K3 (KanR)


 * Construct CBD 6
 * T7 promoter is in the process of cloning
 * dsRed has been ordered


 * Construct CBD 7
 * tet promoter available in the registry: BBa_R0040
 * dsRed has been ordered


 * Construct CBD 8
 * pBad promoter avaiable in the registry: BBa_I0500
 * dsRed has been ordered
 * Arabinose C regulator protein with RBS: BBa_S03550 (available), in pSB1AC3 (A+CR)
 * Stop codon: BBa_B0015, in pSB1AK3 (A+KR)
 * tet promoter available in the registry: BBa_R0040


 * Construct CBD 9
 * &lambda;cI regulated promoter available in the registry:BBa_R0051


 * Construct CBD 10
 * Parts avaiable ....

Constructs for Infector Detector

 * Construct ID 1
 * Entire part minus promoter is currently in Biobricks Registry: BBa_F1610 (available), in pSB1AK3 (AmpR, KanR)
 * pTet is currently in Biobricks Registry: BBa_R0040 (available), in pSB1A2 (AmpR)
 * pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.


 * Construct ID 2
 * Entire part is currently in Biobricks Registry: BBa_J24677 (available), in pSB1A2 (AmpR)
 * Entire part minus GFP reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
 * GFP reporter system is currently in Biobricks Registry: BBa_I13504 (available)
 * pLux can be used with E.coli RNAP in vitro and maybe in veso, and it is repressed by LuxR but activated by AHL:LuxR complex.


 * Construct ID 3
 * Entire part minus Hrp system is currently in Biobricks Registry: BBa_F2620(available) in pSB1A2 (AmpR)
 * pHrpL can be used with E.coli RNAP in vitro and maybe in veso, and it is inducible by HrpR:HrpS:Sigma 54 complex.


 * Construct ID 4
 * Entire part minus dsRed reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
 * dsRed has been ordered


 * Construct ID 5
 * Entire part minus the hrp system and dsRed reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
 * dsRed has been ordered
 * hrpR-hrpS-pHrpL has been ordered

This section contains irrelevant data and should be disregarded: 
 * - style="background:#CCFFCC" align="center"
 * || || BBa_I719??? || hrpR-hrpS & phrpL || Pending || hrpR-hrpS + phrpL || ID detector test construct 3 and final construct 5


 * - style="background:#CCFFCC" align="center"
 * || || BBa_I719??? || Ptet-luxR-Plux & hrpR-hrpS-phrpL || Pending || BBa_F2620 + hrpR-hrpS-phrpL || ID detector test construct 3 and final construct 5


 * - style="background:#CCFFCC" align="center"
 * || || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & GFP || Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + BBa_E0240 || ID detector test construct 3


 * - style="background:#CCFFCC" align="center"
 * || || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & DsRed|| Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + DsRed || ID detector final construct 5