User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/12

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Lyse HEK293 (isolate RNA), 2nd AB WB, Convocal microscopy
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * 2nd Antibody (mouse) on WB, 1:1000 dilution (SHOULD BE 1:10.000)
 * RIPA (Lysis) Buffer (per 1 mL)
 * 1.06 mg β-Glycerolphosphate
 * 1 mL RIPA buffer
 * 8 μL Protease inhibitor mix
 * 1 μL NaF (1 M)
 * 10 μL NaVO3 (10 mM)
 * 12.5 μL PMSF
 * Lysate HEK293 Cells
 * CONVOCAL MICROSCOPY!
 * Isolate RNA of HEK293 Cells

Results

 * Western blot
 * Quick preview of IF results
 * No good picture of Gravin localisation.
 * And no changes in RII with Ht31

Discussion

 * ✅ Ow..

Attachment
   [[Media:12082010_RNA_concentration_HEK293.pdf| RNA concentration HEK293]]


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