User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/24

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Digestion
10 μL DNA 2 μL 10X NEBuffer 4 --> 52 μL 1 μL NdeI          -->  26 μL 7 μL MilliQ        --> 182 μL

37°C for 1 hr.

Expected Patterns

 * Good: 6040, 1416bp
 * Empty: 6645bp

Gel

 * 0.8% Agarose w/ EtBr
 * All colonies gave correct pattern except for 2 and 23


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