IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/08

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Prepare chromosomal DNA extraction from Bacillus
- Grow 2 tubes into LB for
 * IA751
 * IA751 + ECE153 from 03/09 (for tissue kit)
 * IA751 + ECE153 from 02/09
 * IA751 + ECE112 from 05/09

PCR AmyE from ECE112
- Add 12μL of SDW, 5 μL of MM, 1μL + 1μL of each primers (for AmyE back and AmyE front) and 1μL of ECE112


 * Lane1 : HyperladderI
 * Lane4 : AmyE front
 * Lane5 : AmyE front
 * Lane6 : AmyE back
 * Lane7 : AmyE back




 * Results : good band for AmyE front, very low band for AmyE back


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