Livesey:SSPE

20X SSPE BUFFER

3 Liters

525.9g NaCl sodium chloride

82.8g NaH2PO4 sodium phosphate monobasic

28.2g EDTA powder FW=372

bring up to 2400mls with H2O

add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)

autoclave for 20 min

2 Liters

350.6g NaCl sodium chloride

55.2g NaH2PO4 sodium phosphate monobasic

18.8g EDTA powder FW=372

bring up to 1600mls with H2O

add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)

autoclave for 20 min

1 Liters

175.3g NaCl sodium chloride

27.6g NaH2PO4 sodium phosphate monobasic

9.4g EDTA powder FW=372

bring up to 800mls with H2O

add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)

autoclave for 20 min