User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/03

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08/03/10

 * &#x2713; Assemblies: Pc-ATF's and Pc-RFP's + Kozak
 * &#x2713; Minipreps: KFV1 (2)

Minipreps > Check with separate NotI and SpeI digests

--> Keep clone #1; figure out what primer to use for sequencing confirmation (Ian's Actin 5C sequence data)

Assemblies
 * 1) KAH160: Kozak(K0001)/(S/P)/~3200 &#x2713; + (1) KAH109/(X/P)/1123
 * 2) KAH161: " + (2) KAH111/(X/P)/1123
 * 3) KAH162: " + (3) KAH112/(X/P)/1123
 * 4) KAH163: " + (4) KAH113/(X/P)/2107
 * 5) KAH164: " + (5) KAH114/(X/P)/2110
 * 6) KAH165: " + (6) KAH148/(X/P)/967
 * 7) KAH166: " + (7) KAH150/(X/P)/967
 * 8) KAH167: " + (8) KAH151/(X/P)/967
 * 9) KAH168: " + (9) KAH152/(X/P)/1931
 * 10) KAH169: " + (10) KAH1153/(X/P)/1934
 * 11) KAH170: " + (11) KAH115/(X/P)/931

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations


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