IGEM:MIT/2005/Experiments

../Experimental Flowchart/

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Experiment Status
Stock-up MiniPreps grow up B0015, J13002, E0840 overnight B0015, J13002, E0840 miniprep B0015, J13002, E0840 glycerol stock J13002

FecA (full length) PCR bb ends digest FecA + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform overnights for miniprep identify correct clones overnights for freezer stock freezer stocks

Jessica
MalE PCR digest MalE + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones

PhoA PCR with higher annealing temperature digest PhoA+ pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones PhoA' QuikChange

Annie
FecA promoter PCR with current primers with higher annealing temperature PCR with newly designed primers digest FecApro + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock

FecI PCR bb end digest FecI + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock

FecR PCR bb end digest FecR + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock

FecA' QuikChange

FecR' QuikChange

Project Experiments
Input Device: fluorescein Head Receiver Unit: anti-fluorescein scFv Receiver 1: ToxR Receiver 2: FecA Signal Processor: Exp. Here Actuator: Exp. Here

../Experimental Timeline/