KAD062806-Construction of pSB1A3-KB001

PCR KB001/KB002 on pSB1A2-S03155	(230 bp, EcoRI/XbaI, Neb2) Paste into pSB1A3-I13522		(EcoRI/XbaI, Large-~3KB) Product is J0##### (temp name KB001 plasmid) -- KB001	Forward EcoRI to construct Ptet part-making biobrick plasmid ttctggaattcgcggccgcaactagagccaggcatc KB002	Reverse XbaI to construct Ptet part-making biobrick plasmid ccgctTctagAagtgctcagtatc