IGEM:Cambridge/2008/Notebook/Voltage/2008/08/07

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Flame Photometry

 * Various concentrations of KCl were made up in LB to calibrate for LB instead of water.
 * Took OD600 readings of mutants grown in KCl concentrations in shaking incubator overnight
 * They were then spun down, resuspended in SDW and spun again to wash, then resuspended.
 * The resuspended cells were put through the flame photometer to discern internal cell K+ concentration.
 * The supernatant K+ concentration was also measured.

Kdp

 * Ran ligation mixtures on agarose gel and extracted 8kb (supercoiled plasmid) bands.
 * Transformed cells with cleaned up plasmid.

Mutants

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