Notebook:Tk/2008/04/21

Transformation results

 * E. coli transformation was normal 200+ colonies
 * picked six more colonies on replated osmolarity tests

recT gene PCR worked, expected size

 * Phusion in 100 ul final
 * 50 ul Phusion M/M
 * 1.5 ul recT-F
 * 1.5 ul recT-R primers
 * 1 ul 10 ng/ul S. citri genomic DNA (from Renaudin)
 * 46 ul water
 * cycle 98/30s (98/15s 55/15s 68/30s)x30 68/10 min
 * band visible at 900+ bp (expect 915)

Reporter design

 * most existing registry reporters have very bad codon usage
 * one possible exception is GFPmut3.1, E0040, which has pretty good codon usage but two Sau3AI sites