Chang Lab:Notebook/CBE/08/146/2008/12/04

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title

 * Started at 930am


 * E coli culturing
 * Used Biosafety Hood to UV for 20 mins, turned off and left for 5 mins.
 * E coli culture from 3 Dec 08 was removed from shaking incubator (incubated 16 hrs)
 * Under the hood, one loopful of E coli streaked (quadrant streaking method) onto LB(A) plate (immediate culture)
 * Repeat for onto LB(A) slant (stock culture)
 * 2X E coli plate and slant incubated at 37 C overnight (started at 1020am)


 * Medium preparation
 * 


 * Notes
 * Always Parafilm petri dishes when storing
 * Overturn dishes upon storing (for incubation, overturn only when no moisture is visibly found)


 * Failed: using incorrectly prepared medium which did not contain broth in it.


 * }