IGEM:PennState/Labbook/NoahJohnson/2007-7-5

July 5, 2007
 * Ran Ligation (Started 9:45)
 * (+) 20uL 1741015-PSB1A2
 * 2uL T4 Ligase buffer
 * 4ul Vector (P1010 in PSB1A2+S+E)
 * 12uL Insert (I741015+S+E)
 * 1uL T4 DNA Ligase
 * 1uL H2O
 * (-) 20uL PSB1A2
 * 2uL T4 Ligase buffer
 * 4uL Vector
 * 1uL T4 DNA Ligase
 * 13uL H2O


 * Made Motility Plates
 * 0.25g tryptone
 * 0.2g NaCl
 * 0.07g eiken agar
 * 0.15g sodium citrate
 * 25mL water


 * Autoclave 20 mins slow exhaust
 * Added Kanamycin (25uL to 12 plates)
 * Added HSL (25uL to 4 of the Kan plates)
 * Final [eiken] = 0.28%


 * Transformation (Started 12:20)