User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/06

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=Objectives=
 * Check trasnformation
 * Purify plasmid pUX19-XRK5
 * See plasmid in gel.

Transfromation

 * Success.

Plasmid purification

 * Performed with miniprep from Miguel´s Lab.
 * Cell grown up by 6 hours in LB with Km 30. 3 tubes with 5 mL and control.
 * 6 tubes with 2.5 mL each one. 6 tubes were mixed in one tube with 60μL TE-RNAse.


 * Lane 1 Ruler
 * Lane 2 Plasmid



The purpose of this gel is to see concentration to cut plasmid and see if that is the correct plasmid.


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