Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/26

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26 May 2009 Lab Meeting
† Announcements † Flu R01 † Coulter Flu Fraunhofer Project
 * SIGN UP FOR NEW LAB DUTIES WITH SONALI.
 * Please answer my safety emails.
 * PDMS Fixture holder - make more at machine shop.
 * Vacuum chamber needs a new gasket.
 * MicroTAS Abstracts accepted. Papers due June 30th.
 * RNA extraction troubleshooting.
 * Mass Spec of elution? In Chemistry.- We should approach them.
 * Bioanalyzer not working.. why? LOD - Serially dilute our samples and look to see if we can see it when we dilute the contaminants and the DNA/RNA: DID NOT SEE ANYTHING.
 * Absolute Quantification assays: TH this week.
 * FTIR-ATR look at the eluant? There are some organics in the eluant. Restrict the range. Align the laser.
 * Fraun folks coming to get trained next week. RNA isolation.
 * IBC for Fraun flu. in process.

† SEPSIS Project †RNA project † COBRA paper 1: Evap with Sol Gel substrate. paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?
 * Revision submitted 5/20.
 * Jeff visiting week of June 1st
 * Test with sol-gel substrate w/o bacteria (26th)
 * Evap system (JD and JZ)
 * Let's get this to a point where we can publish and move on

† Virus concentration † Valve Array † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR
 * Teddy will train Alex. JD. when he back from vacation.
 * Paper revised.
 * Bonding ongoing with MIT folks
 * Final draft with collaborators.
 * Herringbone masks made.
 * investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
 * IRB still in revision.
 * PCR 2 paper draft.
 * will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)

† RCA/HDA
 * Integrated chip worked(19th)
 * 3 more chips are tested when Sonali back(28th Thu).
 * Valve characterization needed
 * Valve characterization needed

† Silica Optimization (Lambda):


 * Run single PCR product from QQ or Sonali the test with the bioanalyzer


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