Matt Gethers/CRI, Thailand/Labwork/Binding Assay/8.8.08 Run Notes

=8.8.08 Binding Assay Run=

Made up a stock of 10x buffer with DTT: 16.3 &mu;l 10x buffer and 1.63 &mu;l 1 M DTT - (DTT is 10x as concentrated as before). Diluted in water as follows:

I put the dilutions on ice and then prepared the FA cuvettes with the following: Add 900 μl water, 100 μl 10x buffer, 1 μl 0.5 mg/ml polydIdC, and 10 μl 1 M DTT (again note that this DTT concentration is 10x last time). To one cuvette, I added 1 &mu;l of 10 &mu;M HmgA probe. To the second (everything else being the same), I added the same amount of Gpx1 probe. Used HmgR (purified 7.14.08) to titrate.

Data
August 8, 2008 HmgR* Data