User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/10

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Check Transformations
All plates transformed well

Overnight Growth pSB1C3
2 colonies where produced

Overnight growth backbones

 * pSB1A3
 * pSB1K3
 * pSB1T3

Digestion backbones
All plasmids where digested (30µl each) using EcoRI and PstI
 * pSB1A3
 * pSB1K3
 * pSB1T3

Gel backbones
1% Agarose Gel, 10-FEBRURARY-2011-17:44 GMT
 * 1) Lane 1: 10µL  1kbp NEB Quick Ladder
 * 2) Lane 2: 50µl pSB13A - EcoRI / PstI
 * 3)  Lane 3: 5µl pSB13A - plasmid
 * 4) Lane 4: 50µl pSB13K - EcoRI / PstI
 * 5) Lane 5: 5µl pSB13K - plasmid
 * 6) Lane 6: 50µl pSB13T - EcoRI / PstI
 * 7) Lane 7: 5µl pSB13T - plasmid
 * 8) Lane 8: BLANK



Ligation

 * 1) 7µl BBa_katE E|S
 * 2) 7µl BBa_I13504  X|P
 * 3) 3µl pSB13K E|P


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