User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/05

Cellular Adhesion

 * Glycerol
 * Took 1.6ml of liquid culture and created 10% glycerol stocks
 * Signal Sequence #2
 * Signal Sequence #3
 * IgAbc #1
 * Fos #1
 * JunB #3
 * GCN4 #2


 * Miniprep
 * Extracted DNA from remaining liquid culture (~6ml)
 * Eluted in 50ul water


 * Spec
 * Signal Sequence #2: 29.2 ng/ul
 * Signal Sequence #3: 26.4 ng/ul
 * IgAbc #1: 27.7 ng/ul
 * Fos #1: 27.3 ng/ul
 * JunB #3: 30.9 ng/ul
 * GCN4 #2: 28.2 ng/ul


 * Sequencing
 * Template: 8ul DNA, 1.8ul water, .2ul primer (all samples with VF2 and IgAbc with VR)


 * Liquid Culture
 * Grew up cultures in 8ml Amp/Cl LB that didn't work last night (cells died in water) with colony PCR backup plate
 * Fos #3
 * JunB #2