IGEM:metu/2009/Notebook/wound dressing/2009/07/18

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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18.07.2009
1. Measurement of OD with spectrophotometer (600 nm)

Dilution factor : 1/5

OD results; Backbone 1 = 0.098   Backbone 2 = 0.038    Backbone 3 = 0.034

They were very low, so we will make plasmid isolation again.

2. Preparation of Electrophoresis Gel

0.5 gr Agarose + 50 ml 1×TAE buffer + 4 µl EtBr We added EtBr after cool down. TAE electrophoresis buffer = 50× We used 1x TAE. (20ml TAE + 980 ml dH2O)

3. Electrophorese was done. Ladder = 5 µl. 2 µl plasmid + 3 µl dH2O + 1 µl loading dye