WangLab:Inhibitors

Inhibitors

 * 1) Jasplakinolide (molecular probes, J-7473): 4 uM 15 min, or 2 M 15 min, 25nM 4h or 50nM 2h give good morphorlogy
 * ML7: blocking MLCK, 5mM stock, 30 M working concentration.

For HUVECs

 * Cyto D: 0.2 uM, 1 hr
 * Taxol: 0.5 uM, 2 hr
 * Noco: 1 uM, 1 hr

serum starved for 16 hr. Cells were either treated with LPA (200 ng/ml−1) for 30 min (Sigma, Poole, UK), PDGF (25 ng/ml−1) for 30 min (TCS Biologicals, Botolph Claydon, UK), or bradykinin (100 nM) for 3 min (Sigma) or were plated on fibronectin (BD Biosciences) (10 μg/ml) for 1 hr.