Shreffler:Notebook/Staff Meetings/2011/03/01

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Agenda Items

 * EoE
 * Early Risk factors (recruiting, nothing for lab)
 * Biomarkers
 * EDN assays: SK will run comparison of two assays this week with AM
 * Eotaxin has been difficult to measure in saliva in FL's experience; could use CBA platform for other samples
 * AM will meet with MY -- ws to arrange


 * Basophil
 * APC project
 * SP showed data on DR kinetics by flow and localization from the Amnis run:
 * DR increases over 18 hours in an IL-3 dependent fashion
 * DR increases in activation dep manner, but decreases from 20' to 2 h
 * FcERI MFI rapidly falls and staining may become clustered -- from a few images, not quantitative analysis yet
 * cell area increases (and perhaps aggregation) with activation
 * surface CD11b increases


 * Allergen Adjuvant
 * we hope to hear from proteonomics on new sequence information this week
 * BR will run zymosan experiment this week


 * PM Baso
 * AM produced very nice anergy experiment -- key variable to prevent 'carry over' appears to be time; by waiting 90' prior to 2nd stim, controls are perfect
 * experiment demonstrates a degree of pathway-specific, partially non-specific suppression; NP-BSA somewhat stronger anergy-inducing signal, despite weaker stim (DerpNP_06 2/25/11)


 * JAX
 * updated scripts for JAX to run as data comes in, note nec format for pcr data
 * AM to catch up on analysis this month
 * requesting that K at Hopkins adds comment column for lab notes
 * mAb cocktail being sent; new PMA/CaI stim sent by AM


 * PM baso
 * AM to finish analysis this week as study nearing point of analysis


 * Carboplatin
 * no changes


 * U19
 * BR to schedule a meeting with JM