User:Tara K. Luckau/Notebook/Team ConGen/2010/10/29

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 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
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DNA Concentration

 * Tina is prepping mass DNA extraction for optimization (using extra samples with lots of chunky)
 * Want to use these samples to figure out relative DNA concentration to use for all future PCR optimizations
 * Take Tina's extractions and spot checks
 * Run PCR on DNA serial dilution; gel for which concentrations produced product
 * note smallest concentration that worked
 * shove it through NanoDrop
 * Compare that sample's spot check and NanoDrop - that's the brightness of spot we'll want for all future PCRs

Questions

 * since I don't really have a verified working primer set, how can I expect the PCR to work?
 * will necessarily need to run DNA concentration gradient, temp gradient and buffer gradient in same reaction


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