DNA ladder

Prepared DNA ladder

 * NEB's 2-log ladder is a good choice for most samples.
 * Deionized Water
 * Loading dye

Mix NEB 2-log ladder with deionized water to a 100 ng/&mu;L dilution. Add loading dye and store at 4&deg;C. Load 12 &mu;L per lane for 1 &mu;g of DNA.

To make 1.2 mL prepared ladder, combine:
 * 1) 900&mu;L deionized water
 * 2) 100&mu;L 2-log ladder (at 1&mu;g/&mu;L)
 * 3) 200&mu;L loading dye