Jessica Karen Wong/Notebook/2007-7-18

Biobrick Primers

 * E0240:
 * F_New (incl E0240, Spe1): CTTAGTAG.ACTAGT.TCACACAGGAAAGTACTAGATGCG (53.1)
 * R Same as before (incl 3K3/1AK3, EcoRI): TCAGCGAT.GAATTC.CAGAAATCATCCTTAGCGAAAGC (54.2)


 * I2057:
 * F (incl I2057, Spe1): CTTAGTAG.ACTAGT.TCACACAGGAAAGTACTAGATGGC (52.1)
 * R: same as E0240

T9002 E0240 piece
 * E0240-F    8mer spe1 TCACACAGGAAAGTACTAGATGCG 53.1
 * Same as E0240_BB_F

PCR/Gel

 * Ran a gel of E0240-3K3 BB PCR and E0240_T9002 tails PCR - nothing showed up
 * Made a Colony PCR of the 1 colony of F2620-3K3
 * Ran a gel of it and looks good - minipreped for sequencing
 * PCR'ed another 100ul E0240_T9002 tails PCR at 51 with vent and a 1:30 ext time

Sequencing

 * Got some of the redone sequencing from monday
 * E0240-3K3 looks scarred on both sides
 * I2055-3K3 is scared but has a random 1 base insertion towards the end
 * I2055-1AK3 still has no promoter
 * Minipreped I2055-3K3 (that we're pretty sure contains the promoter) for seq
 * Shouldn't put too much faith in these b/c these results still aren't the ones we made glycerols of