User:Meng Xiao He/Notebook/fall08/2008/10/24

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PCR flanking regions
Phusion:
 * cbb3III 5
 * cbb3I 3
 * ccoII 5
 * ccoIII 3
 * DuoII 5
 * DuoI 3

35 cycles:
 * 60°C annealing for 5 cycles, 58°C annealing for 30 cycles, 60°C for 5 cycles
 * 1kb extension time

Mix:
 * 2μL 25μM primer pair mix
 * 25μL 2x master mix
 * colony
 * 23μL H2O


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