IGEM:PennState/Labbook/NoahJohnson/2007-8-20

August 20, 2007

PCR purified the working crp* rxn from friday (0.1uL template crp* DNA with Phusion polymerase)

Restriction digest of crp* and P1010 in psB1A2 with EcoR1 and SpeI

Ran gel of restrictions:
 * All 4 crp* digests worked
 * none of the psB1A2 digests seemed to work

10-6:30