User:Marcel Quint/Notebook/wai cloning/2008/04/25

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] wai cloning
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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investigate methods to pcr/sequence from T-DNA into genomic DNA
→ settled on method by Siebert et al. (1995) NAR using adaptors that prevents amplification of double-stranded adaptor sequences at both ends; this method has been progessively developed into the genome walker kit by clontech → try self-made without kit
 * plasmid rescue
 * walking in uncloned genomic DNA


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