Template:SBB10Project-38352

Daniel Sedor

sbb10: Sleeping beauty (SB100x)
Source: pBca9523-Bca1587 Target Sequence: GKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRKYKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVKRVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHNDHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDILKQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENLWAELKKRVRARRPTNLTQLHQLCQEEWAKIHPTYCEKLVEGYPKRLTQVKQFKGNATKY* Vector: pBjk2741 Short description: <SB100x! Flavor: {<part!} Family: Transposase Your part is derived from PMID 19412179. It is a hyperactive mutant of the originally described sleeping beauty transposase.

sbb39: Magnesium-repressed Promoter
Source: pBjh1601CK-Bjh1380 Vector: pBjk2741 Short description: {PmgtCB} Family: Conditional Promoter This part encodes a Magnesium ion-repressed promoter. In the absence of Mg2+ cations, PmgtCB generates RNA transcripts of downstream DNAs. In the presence of Mg2+, the promoter is shut off by the PhoPQ two-component histidine kinase pathway.

sbb40: Nuclear localization peptide
Source: pBjh1601CK-Bjh1858 Vector: pBjk2741 Short description: {<NIS!} Family: Targeting Peptide This part encodes a peptide that will target fused proteins to the nucleus of eukaryotic cells.