IGEM:MIT/2005/Completed Work

scFv.TT (J07012-14.B0015) digest B0015 E/X, recipient obtain digested scFvs E/S, prefix donor gel purify B0015 ligate J07012-14.B0015 transform overnights for miniprep identify correct clones (digest), streak plates overnights for freezer stock freezer stocks scFv.B0015

scFvs: 4M5.3, S101A, 4420 biobrick ends digest scFv + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform overnights for miniprep identify correct clones (digest) overnights for freezer stock freezer stocks