JCA091306-Construction of lock3l (J23077)

Construction of lock3i (lock3d with 5' extension)
 PCR ca1028F/ca1001 on pJ23119-J23049. . . (865 bp, XbaI/PstI) Sub into pJ23076. . . . . . . . . . . . .(SpeI/PstI, large 2105) Product is pSB3C6-J23077, [Ptet][lock3I][RFP][TT] 