User:Chaoj

=Student Registration/Questionnaire= Please copy the source code from this page to your user page (click on the button above, and copy everything inside the box to your user page). Fill in the answers and print out a copy for next time. You do not need to keep the information on your user page once you've printed it out.

Last Name
Chao

First Name
Jennifer

Preferred name
Jen

Course/Minor
Course 20, minor in Management

Year of Graduation
2009

Telephone #
610-952-3098

Email
chaoj at mit dot edu

Day 4: Genome Refactoring
I refactored the phage genome to remove overlaps and added in restriction enzyme sites, which EcoRV and HindIII cut, between each part, more specifically Gene 8 and the Gene 3 promoter. I changed a few bases, and consequently some codons, but retained the same amino acid sequence while weakening the rbs or promoter. Considerations: 1)Unstuffed by splicing all genes from smaller parts into composite parts; removed overlaps. 2) eliminated promoter of gene 3, by changing a few bases that would not change amino acid sequences but would decrease the strength of the rbs or promoter. 3) add restriction sites between each part BBa_M30058.

New Parts: restriction enzyme sites {| border="1" ! restriction enzyme ! code
 * EcoRV
 * GAT|ATC
 * HindIII
 * A^AGCT_T
 * HindIII
 * A^AGCT_T