IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/02

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Transformation of Bacillus

 * with fresh plates from yesterday, last protocol




 * t0 (IA751) = 100min
 * t0 (IA771) = 110min


 * Transformation

- IA751 on Cm5 and Spc 50 (control DNA less plates)

- IA751 + ECE112 (1μg of DNA) on Cm5

- IA751 + ECE153 (0.6μg of DNA) on Scp50

- IA771 on Cm5 (control)

- IA771 + ECE166 on Cm5


 * Glycerol stocks (about 20 tubes of each)

Check ECE112 stock
- Double digest with XbaI and EcoRI



- Gel : problem only one band (single digest???)