Lissa1: July27-August2

Wednesday, July 27
TY'S plate!!!!!!!!!!!!!!! -DONE
 * 1) Run a gel of the PCR reaction and cut out the pGEV band. - DONE, but pGEV was not visible
 * 2) Pour little gels and debug gel-casting system, store -DONE
 * 3) Run a new pCR of ACLY700 and YAS with more controls overnight -DONE
 * 4) Put in new overnights (again) of parent, Fus3delta, Fus3Kss1delta strains. -DONE
 * 5) Put in LARGE overnight for nocodazole arrests (403 in SCR-u-h), and another one to make more blue-circle samples. -DONE
 * 6) Make media for nocodazole arrests - DONE

Thursday, July 28

 * 1) Run a new gel of the PCR from yesterday -DONE
 * 2) Run yet another gel of yesterday's PCR -DONE
 * 3) Beta-estradiol stocks. - DON'T KNOW HOW!!!
 * 4) Plan induction experiment -DONE
 * 5) Prep proteins (AM) -DONE, but it took them *forever* to grow.., load and run gels, set up transfer - NOT FEASABLE
 * 6) Nocodazole arrest, freeze samples. - DONE
 * 7) Gel extraction - DONE
 * 8) Make buffers -DONE
 * 9) Alpha-arrests -DONE

Friday, July 29

 * 1) Induction experiment using pGEV system - SADLY, DOES NOT EXIST YET
 * 2) Pour new SUMO gel -DONE
 * 3) Do another phosphatase/native extraction prep (with more CIP, buffer, etc), and load on a new SUMO gel.
 * 4) IMAGEQUANT

Weekend, July 30-31

 * 1) Saturday - will be in Western Massachusetts
 * 2) Sunday - will be sailing - evening: set up transfer of phosphatase gel.

Monday, August 1

 * 1) Pour new SUMO gel, prep nocodazole samples and load.
 * 2) Long incubation, washing and imaging of Western blot.
 * 3) Prep samples, load and run 2 small gels, transfer, incubate.
 * 4) Put in overnight of ACLY700.

Tuesday, August 2

 * 1) Long incubation and imaging of Fus3 Western -DONE
 * 2) Transform 403 in to ACLY700 and plate. -DONE
 * 3) PCR ACLY700 DNA, run a gel, gel extract, send for sequencing. -DONE
 * 4) SCBT (probably tomorrow)
 * 5) Order spacer plates. -DONE
 * 6) Math model. -THINKIN'

Wednesday, August 2

 * Cut, transfer of nocodazole gel.
 * 1) Call SCBT? - As of yesterday no longer seems like a good idea
 * 2) Pour 2 small gels. -PENDING...
 * 3) Run a new set of Fus3/activeFus3 gels, set up transfer.
 * 4) Model -NEED PARAMS

Thursday, August 4

 * 1) Wash and image Nocodazole gel
 * 2) Long incubation, wash, image gel from Weds.
 * 3) Get cells of ACLY700-403 plates, grow up overnight.
 * 4) Figure out beta-estradiol stocks.

Friday, August 5

 * 1) Beta-estradiol stocks.
 * 2) Induction experiment.