PLS

Making 2x PLS

 * 1M Tris pH 6.8         1.5ml
 * 10% SDS                6.0ml
 * Glycerol               3.0ml
 * H20                    3.0ml
 * Bromophenol blue       small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20

and