IGEM:British Columbia/Protocols/Transformation Competent Cells

Materials

 * Competent cells
 * Plasmid to be transformed
 * Ice
 * LB medium
 * LB agar plates

Equipment

 * Water bath/heat block at 42°C

Method
NOTE: If working from a miniprep plasmid stock, the concentration will likely be too high and will have to be diluted.
 * Remove 100μL aliquots of competent cells from -80°C freezer and thaw on ice
 * Add 1μL of plasmid to be transformed to the thawed cells and incubate for 30 minutes on ice
 * Heat shock in water bath/heat block for 60 seconds
 * Put cells back on ice for 2 minutes
 * Add 400μL LB medium and incubate at 37°C for 2 hours
 * Plate entire 500μL onto a LB agar plate with the correct antibiotic and spread evenly with a sterile spreader
 * Incubate overnight (<18hrs) at 37°C