IGEM:University of Chicago/2009/Notebook/Organophosphate Hydrolase/2009/07/16

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Thursday, July 16, 2009
Yeast colonies picked last night into 2x - W/0.1% glu

(300 ml 2x -W -U + 6ml ura + 1.5 ml 40% glucose) did not grow very well. Going ot use cultures from 13 July Monday.

1. pCTCON2:

OD 660 = 1.370 cells (107) = 3.64 need cells (10^7) = 0.255 - 0.385 dil = 11 x    get OD660 = 0.26

2. pCTCON2-GFP: OD660 = 1.357 cells (107) = 2.58 need cells (10^7) = 0.255 - 0.385 dil = 11 x  get OD 660 = 0.26

Actual OD 660 after dilution : pCT 0.168 pCT-GFP 0.171 11 a.m.

14:00 Very few cells in both but pCTCON2 does not glow and pCTCON2-GFP does, albeit not enough to photograph

15:30 More cells, but now neither glows.

End of the Day


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