User:Linh N Le/Notebook/2009/07/17

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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To Do

 * Clean the Autoclave
 * What does it mean to "clave"?

PBS

 * Moved PBS to fridge under Andy's advice to prevent things growing in it
 * updated the inventory page

Autoclave

 * Started the Cleaning Process
 * Luckily we had 1 package of Chamber Brite
 * A box of 10 more packages will run ~$40
 * Carolina medical 701675 $39.92]
 * Aria medical $44
 * Zoetech $42
 * ORSupply Product Id# 1535 $43.33

Procedure
Note:Use gloves, this stuff is corrosive
 * 1) Remove all the trays and samples from the autoclave
 * 2) Sprinkle the contents of the Chamber Brite package in a straight line front to back
 * 3) Run a 30min cycle at 273°F (fill water, set to STE, etc)
 * 4) After the pressure goes down to 0psi, open chamber and drain the water reservoir (small adjustable spigot lower left of the opening)
 * 5) Refill the reservoir (opening upper right corner of the top of the autoclave) with DI H2O
 * 6) Run another 30min cycle w/o any Chamber Brite
 * 7) After cycle drain the reservoir again (This seems a bit redundant but that's what the manual says)
 * 8) Turn off Autoclave and allow it to cool
 * 9) Remove tray holder and wipe the inside with a damp cloth
 * 10) Wipe tray holder with damp cloth and return to chamber
 * 11) Fill the chamber with a little bit of water (2-3oz), wipe away water
 * 12) Autoclave good to go

CRLS

 * Andy got the itemized budget in from CRLS and wants me to google spreadsheet it and compare to receipts


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