IGEM:Harvard/2007/Plates

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation)
Plated 6-20-07

Colony Count of Overweekend Ligation
Plated 6/25/07 The following were all done with 275 uL:

Colony Count of Electroporation
Plated 7/11/07

{|
 * align="center" style="background:#f0f0f0;"|Cell Type
 * align="center" style="background:#f0f0f0;"|Volume Plated
 * align="center" style="background:#f0f0f0;"|Colony Count (white)
 * align="center" style="background:#f0f0f0;"|Colony Count (red)


 * AIDA1 + strep, MACS||5 µL||5||0
 * AIDA1 + strep, MACS||50 µL||31||5
 * AIDA1 + strep, MACS||300 µL||235||10
 * AIDA1 + his, MACS||5 µL||0||1
 * AIDA1 + his, MACS||50 µL||18||0
 * AIDA1 + his, MACS||300 µL||206||11
 * AIDA1 + his, MACS||5 µL||0||1
 * AIDA1 + his, MACS||50 µL||18||0
 * AIDA1 + his, MACS||300 µL||206||11
 * AIDA1 + his, MACS||50 µL||18||0
 * AIDA1 + his, MACS||300 µL||206||11
 * AIDA1 + his, MACS||300 µL||206||11

MACS and FACS Cell Sort with AIDA Construct
Plated 7-20-07

MACS Cell Sort with AIDA Construct-All Induced
Plated 8-5-07

MACS Cell Sort with AIDA Sender Constructs
Plated 8-10-07

Note - Red colonies are what we want, white is background