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Averee Chang To my Notebook

Pbad.VioABCDE.term part jtk2914



This part exists but requires an EcoRI/BamHI transfer
The VioABCDE set of genes encodes a biosynthetic pathway for producing violacein, a purple pigment. You'll notice when you work with this that your cells turn dark purple even if you don't put a promoter in front of it. It's very potent! (and also pretty toxic, we're going to figure out why) Your part was converted from a part made by the Cambridge 2009 iGEM team. More information on this pathway is available at their website (http://2009.igem.org/Team:Cambridge). This part already exists, but it is with the wrong vector. You'll need to do an Eco/Bam transfer to create the right plasmid. Your starting material is pBjh1601KC-jtk2914, and we need to subclone it into pBca9523-Bca1144#5. P_sfmC part sbb1126

P_narP part sbb1109