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Eric Hsieh

Ice Nucleation Repeats
Source: pBca1256-Bca1346 Ice nucleation protein is one of our display systems, but it has an interesting feature that may be useful for introducing in other display systems. It has many repeats of: [.........48.residues.repeated.domain..........] /             / |              | \              \            AGYGSTxTagxxssli  AGYGSTxTagxxsxlt  AGYGSTxTaqxxsxlt [16.residues...] [16.residues...]  [16.residues...] From http://www.ebi.ac.uk/interpro/IEntry?ac=IPR000258

You can download the template map for your part [[Media:pBca1256-Bca1346.seq | here]]. You will be making a BglBrick passenger part derived from the navy blue region.

Here is a starter reference for background: PMID: 16233340

Leucine Zipper Peptide IILK
Source: pBca1256-mm011 {}{}{} This part has already been constructed as a BglBricks basic part in plasmid pBca1256. You will be transferring the part using the Gateway reaction into the assembly vector. To do this, you will react the source plasmid with plasmid pBca1254AK. As background, you should read PMID: 12459719.



This part encodes a passenger protein
After performing the Gateway reaction, your product plasmid will be pBca9495AK-partname. To see what the part looks like in the product vector, look at [[Media:JCA_pBca9495AK-Bca1144.seq | this map]] of the RFP-expressing part Bca1144 in pBca9495AK.