IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/27

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Xylose test

 * Preparation of xylose tube

- Add 1mL of culture from yesterday, 1mL of xylose (0.1g/mL) and 8mL of LB and 45μL of Spc (do that for the 4 different colonies)

- Incubate 5hours

- Observation with microscope : some green task, but they do not really match with bacillus cells, not conclusive!

Starch plate
- Add 5mL of iodine solution on starch plate, wait for 1min, throw the liquid

- Result

Ery test
- IA771 alive and IA751 dead, confirmation that we did not mix plates (same conclusion than with starch plate, last line)

Test glycerol stocks of competent bacillus
- Pellet bacillus cells, add 100μL of prewarmed mediumB, add 0.6μg of DNA, incubate 30min at 37°C by shaking, plate


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