IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/24

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] Magnetic Bacteria
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Recovery of mms6 and mamC
Recover mms6 and mamC plasmids from colonies:
 * Recover plasmids from single colonies using Zyppy kit following standard protocol
 * Restriction digest with EcoRI and PstI
 * Run on gel and recover mms6 and mamC DNA

Lane 2 - Hyperladder I Lane 3 - 22 (Voltage) Lane 4 - 26 (Voltage) Lane 5 - mamC Lane 6 - mms6 Result:
 * Two clear bands obtained on gel at around 400-500 bp
 * Gel cut out and put in eppendorf tubes for gel recovery tomorrow