Berglund:Large Scale: 10ml

Overview
Protocol from Mary, as yet untested in our lab.

Materials

 * 20µg/mL linearized plasmid DNA
 * 40mM Tris pH 8.0
 * 10mM DTT
 * 10mM MgCl2
 * 1mM each rNTP
 * 2mM spermidine
 * radioactive nucleotide?
 * T7 RNA poly

Procedure

 * 1) Incubate at 37°C for 5 hours.
 * 2) PAGE purify.