Endy:Screening plasmid/Notebook

Approach

 * 1) Part 1
 * 2) *Construction of High BG-promoter RFP and Hairpin constructs(completed)
 * 3) *Characterization of Inducible GFP (spec out exps on plate reader / LH-FACS)
 * 4) Part 2
 * 5) *Characterization of Inducible RFP (same as inducible GFP exps)
 * 6) *High BG RFP-promoter and Hairpin experiments (completed) (Results: HighBG-promoter,Hairpin)
 * 7) *Based on results from (2) test inducible RFP ( I13520 ) at low and high induction on MOFLO
 * 8) Part 3
 * 9) *Rebuild ESP if necessary based on High BG RFP/Hairpin
 * 10) *Test ESP (same as inducible GFP exps)
 * 11) **Test the flipped FP constructs, cassie's constructs, and also the other version of the ESP where we cut out ccdb and re-ligated.

High BG RFP

 * Endy:Screening plasmid/v1.0/High BG RFP - Figure out why we are seeing a high BG level of RFP in the empty SP

Hairpin effectiveness

 * Endy:Screening plasmid/v1.0/Hairpin - characterize effectiveness of the hairpin to stabilize the mRFP1 transcript

Characterization of Inducible RFP
I13520

Characterization of Empty Screening Plasmid 1.0
To ensure our screening system was operating as expected we measured the expression levels of GFP and mRFP1 when the plasmid did not contain a part (e.g. input equals output).
 * Endy:Screening plasmid/v1.0/RNAseE sites - characterize effectiveness of the RNAseE sites
 * Endy:Screening plasmid/v1.0/Growth rates - characterization of growth rates under different induction levels / conditions
 * Endy:Screening plasmid/v1.0/Steady state - evaluate expression levels at various time points following induction.
 * Endy:Screening plasmid/v1.0/Characterization protocol

Glycerol Boxes

 * Endy:Screening plasmid/v1.0/Glycerol box
 * Endy:Screening plasmid/v2.0/Glycerol box