Laccase Protocols

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Introduction
This protocols are used to screent for and assay laccase activity.

Materials
Bromophenol blue

Materials

 * Gum Guiac or Guiacol
 * 95% Ethanol

Procedure
1. Dissolve either 17mg gum guaiac or 13mg guaiacol into 1ml of 100% ethanol.
 * If your guaiacol is in liquid form then add 12μL to 1ml ethanol.

2. Drop one drops of this solution on a large colony (2 days old for bacteria). 3. Wait four hours and a laccase positive colony should turn purple. 4. Bacteria don't usually like ethanol, so now they're probably dead.

Materials

 * Alkaline Lignin
 * Ferric Chloride
 * Potassium ferric cyanide
 * Apropriate agar growth medium

Materials

 * 2,6-dimethoxyphenol(DMP)
 * sodium malonate