Oneill Lab:WMISH Chemicals

Michael J. O'Neill Lab University of Connecticut Department of Molecular and Cell Biology

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Post-Fix
This solution must be made fresh. Glutaraldehyde is a potent fixative, and should only be used in a chemical fume hood. Do not dispose of excess fix or glutaraldehyde solution in sink.

Hybridization Buffer
Hyb buffer should be made fresh for each hybridization. Excess can be stored at -20&deg;C for use in post-hyb washes. Always preheat to 70&deg;C before use.

TBST
After all materials combined, solution should be filter sterilized.

MABT
After all materials combined, solution should be filter sterilized.

2% Blocking Solution

 * Dissolve components at 63&deg;C with shaking.
 * Store at 4&deg;C

Genius 3
After all materials combined, solution should be filter sterilized.

ProteinaseK Buffer

 * 100 mM Tris pH 8.0
 * 50 mM EDTA pH 8.0
 * Store 4°C