Chang Lab:Notebook/CBE/08/149/2008/12/04

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 * style="background-color: #FFF"|   Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
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 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page


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Logs

 *  Time in: 0930 hrs 
 * E coli culturing
 * Biosafety Hood UV-irradiated for 20 mins, turned off and left for 5 mins (SOP)
 * 3/12 E coli culture removed from shaking incubator (incubate 16 hrs)
 * Under the hood, one loopful of E coli streaked (quadrant streaking method) onto LB(A) plate (as immediate culture)
 * Under the hood, one loopful of E coli streaked (quadrant streaking method) onto LB(A) slant (as stock culture)
 * 2X E coli plate and slant incubated at 37 C overnight (time start: 1020 hrs)


 * Medium preparation
 * Repeat of 3/12 procedure

Notes
 * Always Parafilm petri dishes when storing
 * Overturn dishes upon storing (for incubation, overturn only when no moisture is visibly found)