WangLab:Primer Design

Primer concerns:

 * 1) GC>50%, Tm~66-72oC, both primers should have similar GC percentile and Tm.
 * 2) deltaG (free energy) for hairpin and dimmer close to Zero.

Subclone

 * miniprep, purify with 30 ul water. Take 6 ul, digest, purify with 30 ul water. For subcloning, add 1 ul vector