Knight:Beta-galactosidase assay/Fluorescence

See beta-galactosidase assay for other methods.

This method uses 4-Methylumbelliferyl beta-D-galactopyranoside (MUG) for lacZ detection (Sigma M1633). MUG has the advantage that it gets into the cell. MUG can be detected via fluorescence using 360nm/460nm

Materials

 * 2 mg/ml MUG in DMSO. Use as 10x (final concentration 200 &mu;g/ml)