IGEM:IMPERIAL/2007/Notebook/2007-10-2

Evaporation experient

 * We carried on experiments from yesterday to find a method to reduce evaporation of samples
 * Results???

Test purified luxR

 * Aim: The aim of this experiment was to test if the purified luxR works and if the construct 2 works
 * To test the purified luxR we used these protocols
 * The results of this experiment can be found here
 * The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR.
 * For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used.

Test pT7 in-vivo

 * Aim: The aim of the test was to verify that pT7-GFP expresses in-vivo
 * The test was carried out using these protocols??
 * The results can be found here??