Berk2006-ConjugationTeam3

JL_11/29/06
(Lane 1=1, lane 2=2... etc. Lane 13= ladder)
 * 1) ran gel:

JL_11/27/06
Tecan measurements

JL_11/20/06

 * 1) made -80 of [lock 3n]J23093
 * 2) plated [lock 3n]J23093
 * 3) grew up [lock 3n]J23093
 * 4) organized boxes in freezer

JL_10/13/06
Conjugated EC100d with on Tri Amp
 * 1) J01064 db1K0 #1
 * 2) J01064 db1K0 #2
 * 3) J01064 db1K0 #3
 * 4) J01064 db1K0 #4
 * 5) J23015-J01064

Also I made TriK and TriA plates

JL_10/09/06
plasmid, so plate on TriK)
 * 1) Mate J23016 with Ec100D (see if the trbC knockout transfers its
 * 1) Do genomic minipreps of J23087 (4 clones)
 * 2) Mini and map J23086 (4 clones)
 * 3) Mini and map J23088 (2 clones)

JL_09/29/06

 * 1) Preperative digest of pJ23006-J23006 (AlwnI/Spei, 2068 Long)
 * 2) Preperative digest of psB1A2-J01003 (AlwnI/Xbai, 925 short)
 * 3) 4 Analytical digests(AvrII/SpeI) each of J23074 (want band at 23097) and J23075 (want band at 824)
 * 4) grow up J23016

JL_09/22/06
Today's to do list:
 * 1) stock and mini pSB1AG0-J23078, map EcoRI/SpeI
 * 2) grow up J23055 #1 for electro comp
 * 3) grow up J23016 (trbC::Cm) for the knockout (later gen. mini and PCR with JL16/17)
 * 4) Rlam/J23057 x pSB1A2-J01003 redo

JL_09/20/06
Today I... - mini-preped J23074 and J23075, and pSB1AKG0-b0015 - gen. mini and PCR J23055 - grew up Rlam/J23057 - analytical digest of J23074 and J23075

JCAnderson 22:35, 20 September 2006 (EDT)
Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells.

JL_09/18/06
Today I have to:
 * 1) Grow up J23074 and J23075 (4 clones each)
 * 2) Grow and spot J23055 (43 degrees J23015 x pCP20)
 * 3) Run PCR of J23069 with your traG 20mers