IGEM:Cambridge/2008/Notebook/Voltage/2008/08/27

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osmY RBS

 * Primers containing RBS for osmY Biobrick were received
 * PCR was performed from biobrick J45250
 * Ligated into dephosphorylated vector backbone
 * Colonies transformed

Restriction Digest & Ligation Test

 * Various combinations of restriction digests & T4 phage genome ligation test.
 * All results positive :-)


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