Shreffler:Notebook/Staff Meetings/2010/03/23

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*Wayne G. Shreffler 15:58, 23 March 2010 (EDT):
Met w/ Sarita re: project in the summer

We discussed aims 1 and 2 of her basophil project

Aim 1: Derp2-IgE complex fate.


 * Hypothesis: in tact Derp2 is sequestered by basophils either on surface or intracellular site without degradation in a manner that could subsequently stimulate B cells
 * Use mAbs and rDerp2 to follow antigen fate following cross-linking

Bert and Alex


 * reviewed spending
 * external hard drive
 * freezer

Calls with JAX and Hopkins
 * JAX Thu afternoon
 * Samples expected from JHU -- call next week
 * Alex will send Mouse urine extract to MJ

- R03

NAC experiment


 * pH of 30 uM NAC is very low -- Alex will figure out how to neutralize and measure pH of all dilutions to determine what data we can retain

CPE/ DC


 * Protein ID