User:Tk/Notebook/MF/2009/10/01

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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PCR of pWV01 origin

 * construction of registry J70500 part
 * PCR from
 * pMG36 colony 1 (Kan resistant, no plasmid?)
 * pMG36E colony 1 (Ery resistant)
 * pMG36C colony 1 (Cm resistant)
 * pMG36 colony 2 (Kan resistant, no plasmid)
 * pMG36E colony 2 (Ery resistant)




 * Prep of PCR from Ery resistant bands gives 67 and 89 ng/ul in 50 ul volume


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