IGEM:IMPERIAL/2007/Tutorials/Guide for Engineers

Background
Engineers don't normally possess a detailed knowledge of biochemistry and cell biology. One of the challenges they face when entering the iGEM project is how to start learning about the biological concepts that are involved in synthetic biology.

The aim of this guide is to give an overview of the applicable biological concepts that will be useful for engineers engaging in synthetic biology. It is compiled by the biologists of the iGEM team for the engineers in a hope that the reciprocal exchange of knowledge and expertise would greatly benefit the team in future brainstorming sessions and experimental design and planning.

Biological Concepts

 * Central Dogma of Molecular Biology
 * [[Media:icgems_centraldogma1.ppt|PPT Part I]]
 * Topics discussed: The basics, DNA replication & transcription
 * [[Media:icgems_centraldogma2.ppt|PPT Part II]]
 * Topics discussed: Translation, protein trafficking and cell-cell communications & Criticisms of the central dogma


 * Standard Laboratory Techniques
 * [[Media:icgems_labtech.ppt|PPT]]
 * Topics discussed: Restriction enzymes, gel electrophoresis, blotting techniques-Southern, Northern and Western, DNA sequencing, polymerase chain reaction (PCR), recombinant DNA & gene cloning


 * Glossary of Useful Terms

Questions for Biologists
Unsure about concepts or having an insatiable desire to know more about biology? Questions and comments can be posted and discussed here.