SBB09 Construct46

Construction of mini protein A Z domain mutant

Wobble Ojw001 / Ojw002             (133bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5        (EcoRI/BamHI, L) Product is pBca9495AK-M10018          {}

Ojw001 Forward construction of Z34C basic part CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG Ojw002 Reverse construction of Z34C basic part CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG