RobWarden:M3D1

=Module 3, Day 1 For Next Time=

PCR Cycle

 * 1) The cycle starts with 94 C for 4 min in order to denature all of the DNA and non-Taq protein in the sample.


 * 1) Gradually raising the annealing temperature will help in making sure template DNA is annealing to primers instead of itself, because there is more and more template DNA as PCR continues.


 * 1) It takes more time for the enzyme to polymerize a new strand of DNA than DNA strands to associate/dissociate.


 * 1) If you were amplifying URA3 on genomic DNA, many things could go wrong.
 * 2) *You could amplify the deletion gene if the tails annealed.
 * 3) *You could amplify a gene with a similar amino acid sequence.
 * 4) *You really just get a lot of things you don't want, because the genome is much more likely than a plasmid to have homologous sequences to your primer.

Writing the Introduction
Module 3 Assignment