IGEM:IMPERIAL/2007/Projects/chassis/Testing/L1020

E. coli S30 Extract System for Circular DNA
Contents:
 * 175µl Amino Acid Mixture Minus Cysteine, 1mM
 * 175µl Amino Acid Mixture Minus Methionine, 1mM
 * 175µl Amino Acid Mixture Minus Leucine, 1mM
 * 450µl S30 Extract, Circular (3 × 150µl)
 * 750µl S30 Premix Without Amino Acids
 * 250µl Luciferase Assay Reagent
 * 1ml Luciferase Dilution Reagent
 * 10µl pBESTluc DNA, Circular (1µg/µl)

Storage: Considerations: amounts of magnesium or potassium salts.
 * 70oC
 * Use highly purified DNA templates (e.g., CsCl- or gel-purified).
 * Inhibition of the expression caused by excess salts(NaCl ≥50mM), glycerol (≥1%), or by very small
 * In addition DNA purification kits should use sodium acetate rather than ammonium acetate. Typical yields range