User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/08

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] AU CHEM-570 Lab Prep
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Objective
Continue with ligation attempts

Bench work

 * 1) User:Daniel Catt ran an analytical gel of ligation reactions
 * 2) Re-plate electroporated DH10B
 * 3) * User:Daniel Catt plated remainder of all transformations from yesterday

Transformations

 * 3 colonies on 400μL plate of NovaBlue transformed with V(His)+I
 * 3 colonies on 100μL plate of electroporated DH10B with V(His)+I
 * 1 colony on negative control (V(His) electroporated into DH10B)

Gel
no bands visible for ligation reactions


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