IGEM:VGEM/2007/Notebook/2007-8-1

Expect synthetic DNA from GENEART for melanin project


 * Making Minimal Medium:
 * Add 495mL of distilled water to 3.0g Na2HPO4, 1.5g KH2PO4, 0.25g NaCl, 0.5g NH4Cl
 * Repeat above step in another flask with the addition of 7g of agar
 * Adjust pH to 7.4
 * Autoclave at 121oC


 * Add 3.02g MgSO4 powder to 25mL of water to make 1M solution
 * Add 8g glucose to 32mL water
 * Autoclave


 * Add 1.0mL 1M MgSO4 solution to broth and to agar solutions
 * Add 5.0mL of 20% glucose solution to broth and agar solutions


 * After cooling, pour agar solution into plates

Extracting and plating K12 Cells:
 * Extract K12 cells from glass vial by heating the tip over a bunson burner flame then cold shocking it
 * Incubate tube and plates at 37oC for 24hr
 * Incubate tube and plates at 37oC for 24hr