IGEM:metu/2009/Notebook/wound dressing/2009/08/21

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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21.08.2009
1. We prepared compotent cell (Top 10)

2. We prepared storage for Top 10 (%20 glycerol)

3. We tryed double digestions. The following amounts are added to microtubes; *B0010, 1) water     20.79 µl            2) Buffer     3 µl 3) Enzyme    1;1µl  (EcoR1, Spe1)            4) DNA        3.21 µl 5) Fast AP   1µl         =30 µl

*B0012, 1) water     20.95 µl            2) Buffer     3 µl 3) Enzyme    1;1 µl   (Xba1,Pst1)            4) DNA        3.05 µl 5) Fast AP   1µl         =30 µl

*KGF5(11.08.09), 1) NEB2      6 µl            2) BSA        1 µl 3) Spe1,EcoR1 1;1 µl           4) DNA        41 µl       =50 µl

*LUXR-RBS(05.08.09), 1) NEB2      6 µl            2) BSA        1 µl 3) Xba1,Pst1 1;1 µl            4) DNA        41 µl       =50 µl

*BB12(04.08.09), 1) NEB2      6 µl            2) BSA        1 µl 3) Pst1,EcoR1 1;1 µl           4) DNA        41 µl       =50 µl 4.We couldn't digest