IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/08/19

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Working on LuxY synthesized plasmid from Mr.Gene
In order to test the correct lenght LuxY transformed, I am going to do a PCR reaction using as template the plasmid transformed harboring LuxY and the restriction fragments obtained with said plasmid.

Primers:

Forward:Preffix primer

Reverse:Suffix primer

PCR: Plasmid transformed harboring LuxY

PCR: Fragments obtained from restrictions of Plasmid LuxY transformed

Results LuxY PCRs reactions:
The product obtained from the two PCR reactions was around 2000 nt of size. Considering that the correct size is 820 nt, we are going to repeat the transformation of the plasmid, it's possible that we did a mistake and chosen the wrong plasmid.

I'm going to do another PCR reaction using as template the plasmid from Mr. Gene that harbors LuxY.


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