Jeff Tabor/AVPR1a R3 amplifification

1. Cheek swab into 30uL H20 2. Colony PCR (Taq)

Cycling parameters
99 degrees; 10'

72 degrees

94 degrees, 2' 94 degrees, 30" 45 degrees, 30" 72 degrees, 20" [40 cycles]

72 degrees, 5'