IGEM:Peking/2007/Switch-Notebook/2007-8-7

Ligation and transformation
T1TE^^(A/X)        lacZa-pLX003^^ lacZ-pLX003^^(A/X) (self-ligation-control)

Digestion of plasmids
2uL   T1T2-pCC002 2uL   ClaI 2uL   SalI 10uL   10*H buffer 84uL   H2O -- 100uL   total

2uL   SDY-EGFP=pLX007 2uL   AatII 2uL   XhoI 10uL   T 10uL    BSA 74uL   H2O -- 100uL   total