IGEM:Peking/2007/Count:Gel-Extraction

quick gel purifcation
according to Transgen kit protocol
 * 1) Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be considered as 100uL,add solution GSB,put into 55C water bath until complently merge,it takes about 5 minutes
 * 2) cool the tube with water to adjust the tempertature of the solution to room temperature
 * 3) Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through
 * 4) Add 650 uL WB,13000rpm for 1 min,discard the flow-through
 * 5) 13000rpm for 2 min,discard the flow-through
 * 6) Put the collection tube into a clean EP tube, Add 50 uL 60C ddH2O in the center of the collection tube,standing for 1 min
 * 7) 13000rpm for 1 min.
 * 8) store the DNA solution at -20C