IGEM:British Columbia/Protocols

British Columbia iGEM Team Protocols
Below is a list of the common protocols that we have used during the year:


 * Making Plates or Liquid Media
 * Making Glycerol Stocks
 * Transformations - Adapted from | Openwetware
 * Day/Overnight Cultures
 * Amplifying Biobrick Parts - Modified from Registry Instructions
 * Restriction Digest - From the Endy Lab
 * Biobrick Ligations - A combination of the Endy Lab protocol, OOW Consensus protocol and personal experience
 * Colony PCR
 * Gel Verification
 * 3A Assembly - Modified from OWW Protocol
 * Miniprep
 * Competent Cell Preparation