Nachury:Protocols

Protocols
Under construction

Cell Lines

 * Establishing stable lines with retrovirus
 * Cloning fluorescent cells by FACS
 * Growing and harvesting cells in large amounts

Extractology

 * Hypotonic/N2 cavitation lysis
 * Testis extract
 * Retinal extract
 * Retinal detergent extract

Protein Biochemistry

 * TAP purification of protein complexes from mammalian cells
 * Silver staining
 * Methanol-Chloroform precipitation of proteins
 * TCA precipitation of proteins

Immunofluorescence

 * General guidelines
 * PFA/Saponin

Lipids

 * Preparation of liposomes
 * Protein-Liposomes interaction assays
 * PIP strip blotting assays (fat blot)

DNA

 * Old-school maxiprep