Julius B. Lucks/Meetings and Notes/01162008 T Ham

Meeting with Tim Ham

 * all reagents listed in paper are available
 * inducible Fim and Hin
 * many RBSs
 * diff promoter systems
 * none in biobrick format, but restriction sites are outside of what is important
 * would start off by re-casting into biobricks - then can easily see what can do (take things out, leave things, move around) - test the limiting parameters
 * single inversion works
 * parts of double inversion don't work - not sure why
 * Hin - part of a big family (Gin, etc.) - swappable - all have same core binding site
 * Fim - native to E. coli - no other members in this family
 * FimB - reversible
 * FimE - inverts in one direction
 * project idea - synthetic counters
 * overlapping inversions like a latch (talk to Josh Gilmore from Keasling lab)
 * problem is need at least 4 independent invertases - they don't exist - would have to engineer them so that they don't have cross-talk
 * not clear how would screen or select for
 * engineering Zn-finger binding domains
 * read details of the 1st paper