Shreffler:Notebook/Basophil Imaging

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Nanotubles

 * Reproduce
 * Structure
 * DNase
 * Actin
 * Time lapse
 * Function
 * depolarization (Ca flux)
 * intercellular transport
 * Activation dependence

Team

 * Alex Praslick
 * Matthew Gross
 * Madhan Masilimani
 * Wayne Shreffler

23 June
Alex and I met to discuss priorities for the summer.


 * Reproducibility of activation-dependent putative nanotubes
 * Characterization
 * Physical dimensions
 * Polarization
 * Actin: phalloidin
 * DNA: intercalating dyes, DNase treatment -- consult reference Yousefi_2008
 * time lapse experiments

To Do:


 * order phalloidin
 * order mAbs
 * order new DAPI
 * review and decide on protocols for phalloidin staining as well as DNA staining/ digestion

Ag uptake

 * IgE-dependence
 * Traffic
 * Interaction with T cells

Activation-induced morphological changes

 * correlation of changes with flow
 * non-releaser phenotype
 * polarization
 * phosphorylation events
 * impact on intercellular activation?