User:Pakpoom Subsoontorn/Notebook/Shape Determination in Bacteria/2008/10/26

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Enzymes(genes) involving in building E.coli cell wall

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List

 * AmiA
 * AmiC
 * AmpC
 * AmpH
 * FtsA
 * FtsBL
 * FtsK
 * FtsQ
 * FtsW
 * FtsZ
 * MepA(mepA)
 * MinC
 * MinD
 * MinE
 * MreB
 * MreC
 * MreD
 * PBP1a(ponA)
 * PBP1B(ponB)
 * PBP1c(pbpC)
 * PBP2(pbpA)
 * PBP3(pbpB or ftsI)
 * PBP4(dacB)
 * PBP5(dacA)
 * PBP6(dacC)
 * PBP6b(dacD)
 * PBP7(pbpG)
 * RodA
 * ZipA

Detail
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 * AmiA
 * Class: Alanine amidases
 * Function: degrade cell wall cross like at the septum by cleaving peptide moiety from the MurNAc so contribute to daughter cell separation(1-73, 1-82)
 * Localization: AmiA-GFP was found to be dispersed throughout the periplasm of the cells
 * AmiC
 * Class: Alanine amidases
 * Function: degrade cell wall cross like at the septum by cleaving peptide moiety from the MurNAc so contribute to daughter cell separation
 * Localization: AmiA-GFP was found to be dispersed throughout the periplasm of the small cells but localized almost exclusively at the septal ring of the constricting cells (1-10)
 * AmpC(1-76)
 * Class: beta-lactamase
 * Function: Affect cell shape (1-76)
 * AmpH(1-76)
 * Class: beta-lactamase
 * Function: Affect cell shape (1-76)
 * MepA(1-92)
 * Class: LMW endopeptidase
 * Function: Penicillin-insensitive endopeptidase
 * MreB
 * Function: possibly actin homolog. absence in spherical bacterium. require for rod shape formation in E.coli. Depletion of MreBCD can be suppressed by over expression FtsQAZ (1-98).
 * MreC
 * Function: function with MreB, essential for rod shape formation in E.coli.Depletion of MreBCD can be suppressed by over expression FtsQAZ(1-98).
 * MreD
 * Function: function with MreB, essetial for rod shape formation in E.coli.Depletion of MreBCD can be suppressed by over expression FtsQAZ(1-98).
 * PBP1a(1-19)
 * Function: transglycosylation, transpeptidation (1)
 * PBP1b(1-19)
 * Function: transglycosylation, transpeptidation (1)
 * PBP1c(1-172)
 * Function: transglycosylation; binds to PBPs1B, -2, -3 and MltA (1-172)
 * PBP2
 * Function: elongation-specific TPase (1-183). Exhibit a preference for pentapeptide side-chain (1-148). Possibly help maintaining the diameter of the cell as PBP2 inhibitor, amdinocillin (mecillinam) induces the increment of pole diameter at newly formed cell poles (1-36)
 * Localization: Spot-like pattern along lateral membrane, division site (GFP)(1-36)
 * PBP3 (1-127)
 * Function: cell-division specific TPase (1-183). N-terminal domain is required for folding and stability of penicillin binding module, functioning as an intramolecular chaperone (1-64). Exhibit a preference for tripeptide chain (1-16)
 * Localization: division septum (IF, GFP)(1-200, 1-205). Have some interaction with cell division protein FtsZ,-A,-Q,-L,-W (1-118, 1-204)
 * PBP4 (1-97)
 * Class: LMW endopeptidase
 * Function: control of cell shape, together with PBP5 (1-117)
 * PBP5 (1-20)
 * Class: LMW carboxypeptidase
 * Function: control of cell shape (1-117, 1-129, 1-130). Stain with defective PBP5 has high frequency for branching (1-42)
 * PBP6 (1-20)
 * Class: LMW carboxypeptidase
 * PBP6b (1-7)
 * Class: LMW carboxypeptidase
 * PBP7 (1-75)
 * Class: LMW endopeptidase
 * Function: control of cell shape, together with PBP5 (1-117)
 * RodA (1-78)
 * Class: translocase or flipase
 * Function: possibly transport lipid-linked precursor of PG through cell wall outside. The mutant rodA(Ts) grows as sphere(1-78).Depletion effect can be suppressed by over expression FtsQAZ (1-194).

Reference

 * 1) Scheffers, DJ and Pinho, MG. Bacterial Cell Wall Synthesis: New Insight from Localization Studies. Microbiology and Molecular biology review, Dec. 2005, p585-607


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