User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/01/22

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Realized that when establishing stable source of [ w/Fm7AG ; FRT42D/CyoAg ], potentially included non Cyo Female
 * User:Robert_J._Schiemann/Notebook/Lipsick_Lab/2009/01/20
 * Removed non Cyo female, transferred to new vial.
 * Saved old vial to collect progeny and check.
 * Control cross from yesterday not correct. Forgot to include hsFLP.
 * User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/01/21
 * Threw out the control crosses.
 * Got line with following genotype to create correct control.
 * [hsFLP122; Sp/Cyo] - Does not express Cyo trait.
 * Will cross to [ Fm7AG/Y ; Sc/CyoAg ] and select progeny that express Fm7 Bar eyes and Curly wings.
 * Collected males and virgin females to set up more experimental crosses:
 * [MH107,hsFLP/Fm7Ag ; mRFP, myb+, FRT42D/CyoAg ] x [ Fm7AG/Y ; FRT42D/CyoAg ]

FRT/RFP Recombination

 * Nothing done today


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