IGEM:IMPERIAL/2006/Ligation Page

J37015 (Final Prey Cell)

 * F2620 (6B) + J37034
 * J37024 (insert), cut with XbaI & PstI, expected size = 1544 bp
 * F2620 (6B) (vector), cut with SpeI & PstI, expected size = 1061 bp + 2079 bp (pSB1A2) = 3140 bp
 * Miniprep sizes expected: 2613 bp & 2079 bp (pSB1A2)
 * Status: Completed & Sequenced

J37015RS (Final Prey Cell with Riboswitch)

 * F2620 + (RS + J37034 )
 * Status: Completed (awaiting sequencing results)

J37016 (Final Polycistronic Predator cell Test Construct)

 * J37019 + I13504 (12D)
 * I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp
 * J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2)= 2941 bp
 * Miniprep sizes expected: 1745 bp & 2079 bp (pSB1A2)
 * Status: Completed & Sequenced

J37018

 * J37031 + R0062 (9G)
 * J37031 (insert), cut with EcoRI & SpeI, expected size = 957 bp
 * R0062 (9G) (vector), cut with EcoRI & XbaI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
 * Miniprep sizes expected: 1012 bp & 2079 bp (pSB1A2)
 * Status: Incomplete

J37019 (For Predator Cell)

 * R0062 (9G) + J37033
 * J37033 (insert) cut with XbaI & PstI, expected size = 862 bp
 * R0062 (9G) (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
 * Miniprep sizes expected: 862 bp & 2079 bp (pSB1A2)
 * Status: Completed & Sequenced

J37020 (Final monocistronic predator test construct)

 * J37031 + J37032
 * J37032 (insert) cut with XbaI & PstI, expected size = 938 bp
 * J37031 (vector) cut with SpeI & PstI, expected size = 957 bp + 3189 (pSB1AK3)= 4146 bp
 * Miniprep sizes expected: 1895 bp & 3189 bp (pSB1AK3)
 * Status: J37020 with terminator B0015 has been completed (awaiting sequencing results) however, we are trying to religate this part using the B0014 terminator.

J37022 (Final AiiA test construct)

 * J04500 (16P) + J37024
 * J37024 (insert) cut with XbaI & PstI, expected size = 911 bp (on pSB1AK3 = 3189 bp)
 * J04500 (16P) (vector) cut with SpeI & PstI, expected size = 220 bp + 3189 (pSB1AK3) = 3409 bp
 * Miniprep sizes expected: 1131 bp & 3189 bp (pSB1AK3)
 * Status: Incomplete

J37023 (for predator cell/test construct)

 * AiiA with Immunotag
 * Done by PCR
 * Status: Complete (awaiting sequencing results)
 * Expected Part Length = 816 bp
 * Expected Vector Length = Unknown (Hanging T tail vector)

J37024 (for AiiA predator cell/test construct)

 * J37023 + B0014
 * J37023 (insert) cut with EcoRI & SpeI, expected size = 816 bp
 * B0014 (1G) (vector) cut with EcoRI & XbaI, expected size = 95 bp + 3189 bp (pSB1AK3) = 3284 bp
 * Miniprep sizes expected: 911 bp & 3189 bp (pSB1AK3)
 * Status: Complete (not sequenced)

J37025 (for AiiA predator cell)

 * B0034 (3O) + J37024
 * J37024 (insert) cut with XbaI & PstI, expected size = 911 bp (on pSB1AK3 = 3189 bp)
 * B0034 (3O) (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 (pSB1A2) = 2091 bp
 * Miniprep sizes expected: 923 bp & 2079 bp (pSB1A2)
 * Status: Incomplete

J37026 (Cre/Lox Part Plasmid 1)

 * Lox66 Part
 * Part Size = 34 bp
 * Has XbaI, SpeI, PstI, and EcoRI sites
 * On Blunt ended hanging T tail plasmid

J37027 (Final Cre/Lox Part Plasmid 1)

 * To be made by PCR
 * Part Size = 1127 bp
 * See John Chattaway for details

J37028 (Cre/Lox Part Plasmid 1)

 * Lox71 Part
 * Part Size = 34 bp

J37029 (Cre/Lox Part Plasmid 2)

 * Cre Part
 * Part Size = 1032 bp
 * Received, in fridge
 * Chloramphenicol resistance
 * Will destroy itself once we use it

J37031 (For monocistronic predator test construct)

 * J37019 + B0014 (1I), pLuxR + RBS + LuxR + Terminator
 * J37019 (insert) cut with EcoRI & SpeI, expected size = 862 bp
 * B0015 (vector) cut with XbaI & EcoRI, expected size = 95 bp + 3189 bp (pSB1AK3) = 3284 bp
 * Miniprep sizes expected: 957 bp & 3189 bp (pSB1AK3)
 * Status: Complete (not sequenced)

J37032 (For monocistronic predator test construct)

 * R0062 (9G) + I13504 (12D), pLuxR + RBS + GFP + Terminator
 * I13504 (insert) cut with XbaI & PstI, expected size = 875 bp
 * R0062 (vector) cut with SpeI & PstI, expected size = 55 bp + 2079 bp (pSB1A2) = 2134 bp
 * Miniprep sizes expected: 938 bp & 2079 bp (pSB1A2)
 * Status: Complete

J37033 (RBS + LuxR)

 * B0034 (3O) + C0062 (7A)
 * C0062 (7A) (insert) cut with XbaI & PstI, expected size = 756 bp
 * B0034 (3O) (vector) cut with SpeI & PstI, expected size = 12 bp + 2079 bp (pSB1A2) = 2091 bp
 * Miniprep sizes expected: 799 bp & 2079 bp (pSB1A2)
 * Status: Completed

J37034 (For Prey Cell)

 * C0261 (24A) + I13504 (12D)
 * I13504 (12D) (insert), cut with XbaI & PstI, expected size = 875 bp
 * C0261 (24A) (vector), cut with SpeI & PstI, expected size = 661 bp + 2079 bp (pSB1A2) = 2740 bp
 * Miniprep sizes expected: 1544 bp & 2079 bp (pSB1A2)
 * Status: Completed

J37035 (Final Monocistronic Predator Cell)

 * J37018 + J37025
 * J37025 (insert), cut with XbaI & PstI, expected size = 923 bp
 * J37018 (vector), cut with SpeI & PstI, expected size = 1012 bp + 2079 bp (pSB1A2) = 3141 bp
 * Miniprep sizes expected: 1953 bp & 2079 bp (pSB1A2)
 * Status: Incomplete

J37036 (Final Polycistronic Predator Cell)

 * J37019 + J37025
 * J37025 (insert), cut with XbaI & PstI, expected size = 923 bp
 * J37019 (vector), cut with SpeI & PstI, expected size = 862 bp + 2079 bp (pSB1A2) = 2941 bp
 * Miniprep sizes expected: 1785 bp & 2079 bp (pSB1A2)
 * Status: Incomplete

Ligation Chart
Ligation Chart (ppt.)

AiiA ligation chart

New Ligations