Erickson lab:Notebook/defensin expression/2008/09/15

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Defensin Expression
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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PCR of CAT/RAT Flea

 * I ran PCR using Cat and Rat flea cDNA made previously and using FNde1 and defensin RBamH1 primers.

10μL Master Mix

2μL each primer(5uM)

1μL cDNA template

5μL water

Ran a gel of the PCR products



Lane 1: 2-Log Ladder Lane 2 cat flea-infected Lane 3 cat flea-uninfected Lane 4 rat flea control Lane 5 negative control


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