IGEM:metu/2009/Notebook/wound dressing/2009/08/26

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] WOUND DRESSING
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26.08.2009
1. Isolations of 5,6,8,10 and storages of 2,3,4,5,6,7,8,9,10,11 were made.

2. We tried to make digestions but we couldn't. The following amounts are added to microtubes;

6,       water           9.4  µl                 18.4µl FD Buffer      2  µl                    3 µl Spe1           3 µl          EcoR1      3 µl DNA            5.6 µl                   5.6 µl

8,       water           8.9µl                   17.9 µl FD Buffer      2 µl                     3 µl Xba1           3 µl           Pst1      3 µl DNA            6.1µl                    6.1 µl 2nd part, solution 30 µl enzyme   4 µl buffer   4 µl water    2  µl   =40µl

3. Outcomes of nanodrop; ng/ µl             Abs 5     578.4              4.563             6      531.3              5.168           8      489.6              4.649          10      290.2              2.967

4. Electrophoresis order; 1) leader / c5 / 5 / empty / 10 / c10 / leader  2) leader / 5 / 5 / c5 / c10 / 10 / 10 / leader