Helpful Websites

Home Page
 * National Center for Biotechnology Information

Prefix and Suffix Help
 * Parts Registry of BioBricks

Analyze Your Sequence
 * IDT Integrated DNA Technologies- Check for %GC (40-60) and Melting Temp. (52-68C)

Analzye both Primers
 * NUPACK Nucleic Acid Package- check both primers for hairpins and trouble spots (No Red!!)

Download here
 * Ape- A Plasmid Editor- Useful tool for checking sequences and finding reverse complements