Springer Lab:Protocols

S. cerevisiae Protocols

 * Yeast Nomenclature
 * Yeast Transformation (Gietz) - Our standard yeast transformation protocol.
 * Yeast Transformation (Cold Spring Harbor?) - a variant of the above protocol that differs in final PEG and LiAc concentrations.
 * Yeast Transformation 2 - short version of protocol
 * Transformation Yeast 96 well
 * Quick and easy isolation of genomic DNA from Yeast

Recipes
Growth media For yeast transformations
 * YNB media
 * 50% PEG
 * 6x loading dye

Antibiotics

 * Antibiotics

High Throughput Techniques

 * Tips on handling plates

Isothermal Assembly

 * Isothermal Assembly
 * Isothermal Kits

Flow Cytometry

 * FCS file format

Lab Maintenance

 * Cleaning Glass Beads