IGEM:MIT/2006/Autotrophic E.coli/Biobrick Calvin cycle enzymes

Background

 * prkA contains a Pst1 site
 * rbcL contains an EcoR1 site

Procedure

 * Miniprep AE4 (this will provide a plasmid prep of both enzymes).
 * Site-directed mutagenesis of the prkA and rbsLS
 * Sequence verify that we got mutations
 * Cut out the coding regions with primers containing BB ends.
 * Clone into BB vector.