User:Matthewmeisel/Notebook/2006-6-12

DNA nanostructure reaction PCR
 * standard protocol with materials from Shawn

Transformation of standard components
 * goal: insert three BioBrick plasmids (already containing BioBricks) into E. coli in order to amplify them
 * a positive control (E7104) with the T7 promoter upstream of GFP
 * the lac operon (R0010)
 * GFP (E0241)
 * transformation protocol:
 * 1) 1  each of E7104, R0010, E0241, and water into separate microcentrifuge tubes that each contain 30  of competent cells
 * 2) Incubated the tubes on ice for 20 min.
 * 3) Heat shocked the tubes at 42&deg;C for 30 s.
 * 4) Added 200  SOC to each tube.
 * 5) Incubated, with shaking, at 37&deg;C for 1 h.
 * 6) Plated the mixture on carbomycin plates and incubated at 37&deg;C for 24 h.