IGEM:Peking/2007/Switch-Notebook/2007-7-23

Experimenter: CXY

We have been trying to connect lacZa into pLX007/8/9 for three weeks but still failed. We decide to repeat the entire process carefully.

Overnight ligation of pLX008-lacZa
pLX008 and lacZa are both digested by XhoI and SalI.