Construction Files

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Gene Synthesis of B09ig1 {<azo1653 AtD>}
Pool O09ig1 through O09ig26, assemble by PCA PCR O09ig1/O09ig26 on PCA reaction       (860, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig1   {<azo1653 AtD>}

O09ig1    PCA assembly of {<azo1653 AtD>} (B09ig1) CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC O09ig2    PCA assembly of {<azo1653 AtD>} (B09ig1) TAGCACCAGCGTTACCGTCGTCGTTCATACGCTGAGTGCCAGAAGACATCTGG O09ig3    PCA assembly of {?azo1653 AtD>} (B09ig1) CGACGGTAACGCTGGTGCTACCACCCAGGACTTCGACGGTGCTCTGATCGGTG O09ig4    PCA assembly of {?azo1653 AtD?} (B09ig1) CAACACGCCAGTCACCGAAAGCCGGCAGGTCACCACCGATCAGAGCACCGTCG O09ig5    PCA assembly of {?azo1653 AtD?} (B09ig1) TTTCGGTGACTGGCGTGTTGGTGCTGCTATCGGTTACGACATCGCTCAGGTTT O09ig6    PCA assembly of {?azo1653 AtD?} (B09ig1) TGGTTTCACCCTCTGCCTGACGAGAACCAACAGAAACCTGAGCGATGTCGTAA O09ig7    PCA assembly of {?azo1653 AtD?} (B09ig1) TCAGGCAGAGGGTGAAACCAAAACCCGTCGTTTCGCTCTGTACTCTGGTAAAT O09ig8    PCA assembly of {?azo1653 AtD?} (B09ig1) CACGCAGAGAGTTACCGTCAGCCAGTTCGAAAGATTTACCAGAGTACAGAGCG O09ig9    PCA assembly of {?azo1653 AtD?} (B09ig1) TGACGGTAACTCTCTGCGTGTTTTCGGTGGTGCTGCTTACTCTCTGCACCGTC O09ig10    PCA assembly of {?azo1653 AtD?} (B09ig1) CGTCGATCAGAGAAACGTCACGAGCAGATTCCAGACGGTGCAGAGAGTAAGCA O09ig11    PCA assembly of {?azo1653 AtD?} (B09ig1) TGACGTTTCTCTGATCGACGGTGTTGAACACCTGACCCGTGGTTACGACGTTG O09ig12    PCA assembly of {?azo1653 AtD?} (B09ig1) GGTAAGCCAGTTCACCGAAGAGTTGACGGGTAACAACGTCGTAACCACGGGTC O09ig13    PCA assembly of {?azo1653 AtD?} (B09ig1) CTTCGGTGAACTGGCTTACCGTCTGGGTCTGGGTGAAAAATCTCACGTTGAAC O09ig14    PCA assembly of {?azo1653 AtD?} (B09ig1) CACGCTGAGCAACACGCATCAGACCAACGAACGGTTCAACGTGAGATTTTTCA O09ig15    PCA assembly of {?azo1653 AtD?} (B09ig1) GATGCGTGTTGCTCAGCGTGCTGACGGTTTCCAGGAAGAAGGTGGTTCTGCTG O09ig16    PCA assembly of {?azo1653 AtD?} (B09ig1) GCAGAGCGTTACGCTGAGAGTCACCAGCCAGAGCAGCAGAACCACCTTCTTCC O09ig17    PCA assembly of {?azo1653 AtD?} (B09ig1) CTCTCAGCGTAACGCTCTGCTGATCTCTACCCTGGGTGCTCGTGGTCAGCACG O09ig18    PCA assembly of {?azo1653 AtD?} (B09ig1) TCAGTTCGATGTCCAGACCACCCAGGTTAACAGCGTGCTGACCACGAGCACCC O09ig19    PCA assembly of {?azo1653 AtD?} (B09ig1) TGGTCTGGACATCGAACTGAAAGGTTCTGTTACCTGGCGTCACCTGGAAGGTG O09ig20    PCA assembly of {?azo1653 AtD?} (B09ig1) CAGACAGACGCAGACCCAGTTCCGGACGAACGTCACCTTCCAGGTGACGCCAG O09ig21    PCA assembly of {?azo1653 AtD?} (B09ig1) ACTGGGTCTGCGTCTGTCTGGTGGTGCTCGTTTCACCGTTATGGGCACTGAAC O09ig22    PCA assembly of {?azo1653 AtD?} (B09ig1) CGTTCAGGGTCAGCAGCATAGAGTCACGCGGCAGTTCAGTGCCCATAACGGTG O09ig23    PCA assembly of {?azo1653 AtD?} (B09ig1) TATGCTGCTGACCCTGAACGCTAACTACGACCTGATGGCTGGTGTTGTTCTGA O09ig24    PCA assembly of {?azo1653 AtD?} (B09ig1) CAGAGTCAGAGGTCATAGCGTTAACACCAACGTTCAGAACAACACCAGCCATC O09ig25    PCA assembly of {?azo1653 AtD?} (B09ig1) CGCTATGACCTCTGACTCTGGTAACGCTGCTGCTCTGGTTGCTAACCTGCGCT O09ig26    PCA assembly of {?azo1653 AtD?} (B09ig1) CGTTAGGATCCGTTCATTTTCCAGCGCAGGTTAGCAACCAGA

Gene Synthesis of B09ig27 {<Nmul_A1566 AtD>}
Pool O09ig27 through O09ig56, assemble by PCA PCR O09ig27/O09ig56 on PCA reaction       (848, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig27   {?Nmul_A1566 AtD?}

O09ig27    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CCATAGAATTCATGAGATCTGCTTTCTGGACCCGTACCTTCGGTTC O09ig28    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTTCATGTCACCAGAACGGTGACCGAAAGAACCGAAGGTACGGGTCCA O09ig29    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCGTTCTGGTGACATGAACGCTGCTCGTATCAACCGTAACATCGGTG O09ig30    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAGGGTGTCACCACCCATGAAGAAACCACCGATGTTACGGTTGATA O09ig31    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ATGGGTGGTGACACCCTGGTTGCTGACCTGCTGCGTCTGGGTATCGCT O09ig32    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTTGAAAGAAGAGTTAGAGTAACCACCAGCGATACCCAGACGCAGCA O09ig33    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTCTAACTCTTCTTTCAACGTTAACGAACGTTTCTCTACTGGCTCTTC O09ig34    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCGTAAGCGGTAACGTGGTAGTTGTCAGAAGAGCCAGTAGAGAAACG O09ig35    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCACGTTACCGCTTACGGTGGCACTCGTTGGCACGCTCTGGGTCTGC O09ig36    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TCGTGCCAGGTGTAACCACCACCGAAACGCAGACCCAGAGCGTGCCAA O09ig37    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GGTGGTTACACCTGGCACGACTTCGAAACCAACCGTAATATTCTGTTT O09ig38    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CACCTTTAGCCTGGTTGTTGAAACCTGGAAACAGAATATTACGGTTGG O09ig39    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CAACAACCAGGCTAAAGGTGACTACCGTGGTCGTACTGGCCAGGTTTT O09ig40    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GAACGGCAGTTCGTAACCCAGTTCACCGAAAACCTGGCCAGTACGACC O09ig41    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGGGTTACGAACTGCCGTTCAAAAACGTTTCTCTGGAACCGTTCGCTG O09ig42    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TTGGTAGCCAGGTTAACGTAAGCAACACCAGCGAACGGTTCCAGAGAA O09ig43    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TACGTTAACCTGGCTACCAAAGGTTTCCAGGAACGTGGTGGTATCGCT O09ig44    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTTCGTTGTCACGAGAAGAAGACAGAGCAGCGATACCACCACGTTCCT O09ig45    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TTCTTCTCGTGACAACGAAGGTGTTACCTACTCTACCGTTGGTATGAA O09ig46    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAGCGGTAGAGAACATAGAAGCAGCGTTCATACCAACGGTAGAGTA O09ig47    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CTATGTTCTCTACCGCTGGTGGCACTACCACCCGTCTGCGTGGTTCTC O09ig48    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACAGAGTCGAAAGCGTGACGCCAACCCAGAGAACCACGCAGACGGGTG O09ig49    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTCACGCTTTCGACTCTGTTCCGACCCGTTCTTCTGTTGCTTTCTCT O09ig50    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGCCAGCGATACCGAAAGCAGAACCACCAGAGAAAGCAACAGAAGAAC O09ig51    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TGCTTTCGGTATCGCTGGCACTCCGATCGCTAAAGACGCTATGATCAT O09ig52    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) ACCAACAGAAGCGTCCAGACCAGCACCGATGATCATAGCGTCTTTAGC O09ig53    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTCTGGACGCTTCTGTTGGTAAAAACGCTATCCTGGGTATCGCTTACA O09ig54    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) TCAACAACGTTAGAGAAAACCTGGCCAGTGTAAGCGATACCCAGGATA O09ig55    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) GTTTTCTCTAACGTTGTTGACAACGGTGTTCGTGCTAACCTGGACTGG O09ig56    PCA assembly of {?Nmul_A1566 AtD?} (B09ig27) CGTTAGGATCCGAAACGCCAGTCCAGGTTAGCACGAA

Gene Synthesis of B09ig57 {<OprF AtD>}
Pool O09ig57 through O09ig78, assemble by PCA PCR O09ig57/O09ig78 on PCA reaction       (710, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig57   {?OprF AtD?}

O09ig57    PCA assembly of {?OprF AtD?} (B09ig57) CCATAGAATTCATGAGATCTGACTCTAAAATGACTGGCGCTTACGGTC O09ig58    PCA assembly of {?OprF AtD?} (B09ig57) AGAGGTAGAGTCAGAGATACCTTTTTCGATCTGACCGTAAGCGCCAGTCATT O09ig59    PCA assembly of {?OprF AtD?} (B09ig57) GTATCTCTGACTCTACCTCTGTTGGTCTGATGCTGGGTGGTAACAACAACAA O09ig60    PCA assembly of {?OprF AtD?} (B09ig57) TACCTTTCAGGTCAGAGGTAGCGATGTCAACTTTGTTGTTGTTACCACCCAG O09ig61    PCA assembly of {?OprF AtD?} (B09ig57) TACCTCTGACCTGAAAGGTAACTCTGCTTACCTGGGTGGTTACGTTAAACAC O09ig62    PCA assembly of {?OprF AtD?} (B09ig57) ATACCAGCGATACCACGGAAAGCACCTTTGTCGTGTTTAACGTAACCACCCA O09ig63    PCA assembly of {?OprF AtD?} (B09ig57) TTCCGTGGTATCGCTGGTATCGGTATCCAGTACTCTGACTGGGACTCTCACC O09ig64    PCA assembly of {?OprF AtD?} (B09ig57) GTTTTCTTTGTAAGAGTCAGACATGGTGTTACGGTGAGAGTCCCAGTCAGAG O09ig65    PCA assembly of {?OprF AtD?} (B09ig57) CTGACTCTTACAAAGAAAACTACTCTGACCGTGGTCTGAACATCTACGCAGA O09ig66    PCA assembly of {?OprF AtD?} (B09ig57) GACCTTCGGTCAGTTCTTTGGTGTATTTACCCTCTGCGTAGATGTTCAGACC O09ig67    PCA assembly of {?OprF AtD?} (B09ig57) CAAAGAACTGACCGAAGGTCTGTTCCTGGAACCGAAAGTTGGTGTTTCTTAC O09ig68    PCA assembly of {?OprF AtD?} (B09ig57) GATTCGTTGATAGATTCCTGTTTGATGTAGTCGTAAGAAACACCAACTTTCG O09ig69    PCA assembly of {?OprF AtD?} (B09ig57) CAGGAATCTATCAACGAATCTAAAGACAAAGCTCTGGCTCTGGAAGTTGACG O09ig70    PCA assembly of {?OprF AtD?} (B09ig57) AACGTTACCAGACAGGGTGTCGAAGTCTTTACCGTCAACTTCCAGAGCCAGA O09ig71    PCA assembly of {?OprF AtD?} (B09ig57) ACACCCTGTCTGGTAACGTTGGTCTGGACCTGCGTAAAGAAATCGTTACCGA O09ig72    PCA assembly of {?OprF AtD?} (B09ig57) TAACACCAGCGGTCAGGTTGTGTTTACCTTTTTCGGTAACGATTTCTTTACG O09ig73    PCA assembly of {?OprF AtD?} (B09ig57) CAACCTGACCGCTGGTGTTAACTACACCCGTATCCTGTCTGGTGCTGACGAA O09ig74    PCA assembly of {?OprF AtD?} (B09ig57) GAGTTACCACCGAAGTTGCCAGTCAGGTTGTCTTCGTCAGCACCAGACAGGA O09ig75    PCA assembly of {?OprF AtD?} (B09ig57) GGCAACTTCGGTGGTAACTCTTTCGATATTCTGGTTCCGCAGAAAACCAAAG O09ig76    PCA assembly of {?OprF AtD?} (B09ig57) GATGTCGTATTTGATACCAACAGAAACGTTGTCTTTGGTTTTCTGCGGAACC O09ig77    PCA assembly of {?OprF AtD?} (B09ig57) TTGGTATCAAATACGACATCGAACTGGAAACTGGCGTTATGCTGGGTGCTAA O09ig78    PCA assembly of {?OprF AtD?} (B09ig57) CGTTAGGATCCGTAAGAAACTTTAGCACCCAGCATAACGCC

Gene Synthesis of B09ig79 {<cl02365 AtD>}
Pool O09ig79 through O09ig112, assemble by PCA PCR O09ig79/O09ig112 on PCA reaction       (854, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig79   {?cl02365 AtD?}

O09ig79    PCA assembly of {?cl02365 AtD?} (B09ig79) CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA O09ig80    PCA assembly of {?cl02365 AtD?} (B09ig79) CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA O09ig81    PCA assembly of {?cl02365 AtD?} (B09ig79) ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT O09ig82    PCA assembly of {?cl02365 AtD?} (B09ig79) CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT O09ig83    PCA assembly of {?cl02365 AtD?} (B09ig79) GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT O09ig84    PCA assembly of {?cl02365 AtD?} (B09ig79) CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC O09ig85    PCA assembly of {?cl02365 AtD?} (B09ig79) AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT O09ig86    PCA assembly of {?cl02365 AtD?} (B09ig79) ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG O09ig87    PCA assembly of {?cl02365 AtD?} (B09ig79) GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT O09ig88    PCA assembly of {?cl02365 AtD?} (B09ig79) CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG O09ig89    PCA assembly of {?cl02365 AtD?} (B09ig79) CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT O09ig90    PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC O09ig91    PCA assembly of {?cl02365 AtD?} (B09ig79) ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC O09ig92    PCA assembly of {?cl02365 AtD?} (B09ig79) GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT O09ig93    PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC O09ig94    PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT O09ig95    PCA assembly of {?cl02365 AtD?} (B09ig79) GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA O09ig96    PCA assembly of {?cl02365 AtD?} (B09ig79) GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT O09ig97    PCA assembly of {?cl02365 AtD?} (B09ig79) ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG O09ig98    PCA assembly of {?cl02365 AtD?} (B09ig79) GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA O09ig99    PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC O09ig100    PCA assembly of {?cl02365 AtD?} (B09ig79) CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT O09ig101    PCA assembly of {?cl02365 AtD?} (B09ig79) TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC O09ig102    PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA O09ig103    PCA assembly of {?cl02365 AtD?} (B09ig79) ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG O09ig104    PCA assembly of {?cl02365 AtD?} (B09ig79) TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA O09ig105    PCA assembly of {?cl02365 AtD?} (B09ig79) CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC O09ig106    PCA assembly of {?cl02365 AtD?} (B09ig79) GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG O09ig107    PCA assembly of {?cl02365 AtD?} (B09ig79) TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA O09ig108    PCA assembly of {?cl02365 AtD?} (B09ig79) GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC O09ig109    PCA assembly of {?cl02365 AtD?} (B09ig79) AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT O09ig110    PCA assembly of {?cl02365 AtD?} (B09ig79) CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA O09ig111    PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC O09ig112    PCA assembly of {?cl02365 AtD?} (B09ig79) CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT

Gene Synthesis of B09ig113 {<VtaA11 AtD>}
Pool O09ig113 through O09ig120, assemble by PCA PCR O09ig113/O09ig120 on PCA reaction       (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig113   {?VtaA11 AtD?}

O09ig113    PCA assembly of {?VtaA11 AtD?} (B09ig113) CCATAGAATTCATGAGATCTAACAACAAAGCTCTGCGTGCTGGTATCG O09ig114    PCA assembly of {?VtaA11 AtD?} (B09ig113) CCTGCGGCAGACCAGCAGCAGCGTTAGAACCAGCGATACCAGCACGCAGAGCT O09ig115    PCA assembly of {?VtaA11 AtD?} (B09ig113) TGCTGCTGGTCTGCCGCAGGTTTATATTCCGGGTAAATCTATGGTTGCTGTTT O09ig116    PCA assembly of {?VtaA11 AtD?} (B09ig113) CCAGAGCAGACTGACCTTTGAAAGTGCCAGCAGAAACAGCAACCATAGATTTA O09ig117    PCA assembly of {?VtaA11 AtD?} (B09ig113) CAAAGGTCAGTCTGCTCTGGCTGTTGGTTACTCTCGTGCTTCTGACAACGGTA O09ig118    PCA assembly of {?VtaA11 AtD?} (B09ig113) TGTTAGCGTTACCTTGGAGTTTCAGGATCAGTTTACCGTTGTCAGAAGCACGA O09ig119    PCA assembly of {?VtaA11 AtD?} (B09ig113) ACTCCAAGGTAACGCTAACACCTCTGGTGAAATGGGTGGTTCTGTTGGTGTTG O09ig120    PCA assembly of {?VtaA11 AtD?} (B09ig113) CGTTAGGATCCCCACTGGTAACCAACACCAACAGAACCACCC

Gene Synthesis of B09ig121 {<Hag AtD>}
Pool O09ig121 through O09ig128, assemble by PCA PCR O09ig121/O09ig128 on PCA reaction       (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig121   {?Hag AtD?}

O09ig121    PCA assembly of {?Hag AtD?} (B09ig121) CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT O09ig122    PCA assembly of {?Hag AtD?} (B09ig121) CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG O09ig123    PCA assembly of {?Hag AtD?} (B09ig121) TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG O09ig124    PCA assembly of {?Hag AtD?} (B09ig121) CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA O09ig125    PCA assembly of {?Hag AtD?} (B09ig121) CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC O09ig126    PCA assembly of {?Hag AtD?} (B09ig121) TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA O09ig127    PCA assembly of {?Hag AtD?} (B09ig121) AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG O09ig128    PCA assembly of {?Hag AtD?} (B09ig121) CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT

Gene Synthesis of B09ig129 {<Pcryo_1225 AtD>}
Pool O09ig129 through O09ig136, assemble by PCA PCR O09ig129/O09ig136 on PCA reaction       (272, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig129   {?Pcryo_1225 AtD?}

O09ig129    PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CCATAGAATTCATGAGATCTGTTGAAGACGACGCTAACGCTGGT O09ig130    PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GCGGCAGAGAAGACATAGCCATAGCAGCAGAGATACCAGCGTTAGCGTCGTCTT O09ig131    PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GGCTATGTCTTCTCTGCCGCAGGCTTACATCATCGGTAAACCGATGATCGGTGG O09ig132    PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) AACAGCAGATTCACCGTTGTAGGTAGCGATACCACCACCGATCATCGGTTTACC O09ig133    PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) ACAACGGTGAATCTGCTGTTGCTATCGGTTTCTCTAAAATGTCTAACGACGGTC O09ig134    PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) GTGTCAGCAGTGCCGTTCAGTTTCATAACCCAACGACCGTCGTTAGACATTTTA O09ig135    PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CTGAACGGCACTGCTGACACTCAAGGCAATGTAGGTGCTGCTATCGGTGCTGGT O09ig136    PCA assembly of {?Pcryo_1225 AtD?} (B09ig129) CGTTAGGATCCGTCGAAGTGGAAACCAGCACCGATAGCAGCAC

Gene Synthesis of B09ig171 {<gliadin binding scFv>}
Pool O09ig171 through O09ig200, assemble by PCA PCR O09ig171/O09ig200 on PCA reaction       (842, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig171   {?gliadin binding scFv?}

O09ig171    PCA assembly of {?gliadin binding scFv?} (B09ig171) CCATAGAATTCATGAGATCTGCTCAGGTTCAACTCGTTCA O09ig172    PCA assembly of {?gliadin binding scFv?} (B09ig171) ACCCGGCTGAACAACACCACCACCAGACTGAACGAGTTGAACCTGAGC O09ig173    PCA assembly of {?gliadin binding scFv?} (B09ig171) GTGGTGTTGTTCAGCCGGGTCGTTCTCTGCGTCTGTCTTGCGCTGCTT O09ig174    PCA assembly of {?gliadin binding scFv?} (B09ig171) ATATCGTAGATAGAGAAAGAGAAACCAGAAGCAGCGCAAGACAGACGC O09ig175    PCA assembly of {?gliadin binding scFv?} (B09ig171) TCTTTCTCTATCTACGATATGTACTGGGTTCGTCAGGCTCCGGGTAAA O09ig176    PCA assembly of {?gliadin binding scFv?} (B09ig171) TAGAGATAACAGCAACCCATTCCAGACCTTTACCCGGAGCCTGACGAA O09ig177    PCA assembly of {?gliadin binding scFv?} (B09ig171) ATGGGTTGCTGTTATCTCTACCGACGGTTCTAACAAATTCTACGCTGA O09ig178    PCA assembly of {?gliadin binding scFv?} (B09ig171) AGAGATGGTGAAACGACCTTTAACAGAGTCAGCGTAGAATTTGTTAGA O09ig179    PCA assembly of {?gliadin binding scFv?} (B09ig171) AAGGTCGTTTCACCATCTCTCGTGACAACTCTAAAAACACCCTGTACC O09ig180    PCA assembly of {?gliadin binding scFv?} (B09ig171) TCCTCAACACCCAGAGAGTTCATTTGGAGGTACAGGGTGTTTTTAGAG O09ig181    PCA assembly of {?gliadin binding scFv?} (B09ig171) AACTCTCTGGGTGTTGAGGATACCGCTGTTTACTACTGCGCTCGTGAA O09ig182    PCA assembly of {?gliadin binding scFv?} (B09ig171) CACCCCATTCTTCGTAAGAACCGTCGATTTCACGAGCGCAGTAGTAAA O09ig183    PCA assembly of {?gliadin binding scFv?} (B09ig171) TTCTTACGAAGAATGGGGTGACTACTGGGGTCAGGGCACTCTGGTTTC O09ig184    PCA assembly of {?gliadin binding scFv?} (B09ig171) CGGAGAGGTTGGGGAAGCAGAAGAAACAGAAACCAGAGTGCCCTGACC O09ig185    PCA assembly of {?gliadin binding scFv?} (B09ig171) CTGCTTCCCCAACCTCTCCGAAACTGGAAGAAGGTGAGTTTTCCGAGG O09ig186    PCA assembly of {?gliadin binding scFv?} (B09ig171) GGCTGGGTCAGAACAGACTGAACACGAGCCTCGGAAAACTCACCTTCT O09ig187    PCA assembly of {?gliadin binding scFv?} (B09ig171) CAGTCTGTTCTGACCCAGCCGCCGTCTGTTTCTGGTGCTCCGGGTCAG O09ig188    PCA assembly of {?gliadin binding scFv?} (B09ig171) AAGAGCCAGTGCAAGAGATGGTAACACGCTGACCCGGAGCACCAGAAA O09ig189    PCA assembly of {?gliadin binding scFv?} (B09ig171) CATCTCTTGCACTGGCTCTTCTTCTAACATCGGTGCTGGTTACGACGT O09ig190    PCA assembly of {?gliadin binding scFv?} (B09ig171) AGTGCCCGGGAGATGTTGATACCAGTGAACGTCGTAACCAGCACCGAT O09ig191    PCA assembly of {?gliadin binding scFv?} (B09ig171) ATCAACATCTCCCGGGCACTGCTCCGAAACTGCTGATCTACGGTAACA O09ig192    PCA assembly of {?gliadin binding scFv?} (B09ig171) CGGTCCGGAACACCAGACGGACGGTTTTTGTTACCGTAGATCAGCAGT O09ig193    PCA assembly of {?gliadin binding scFv?} (B09ig171) CCGTCTGGTGTTCCGGACCGTTTCTCTGGTTCTAAATCTGGCACTTCT O09ig194    PCA assembly of {?gliadin binding scFv?} (B09ig171) CTTGGAGGCCAGTGATAGCCAGAGAAGCAGAAGTGCCAGATTTAGAAC O09ig195    PCA assembly of {?gliadin binding scFv?} (B09ig171) GGCTATCACTGGCCTCCAAGCTGAGGATGAAGCTGACTACTACTGCGC O09ig196    PCA assembly of {?gliadin binding scFv?} (B09ig171) CCAAACGTTCAGAGAGTCGTCCCAAGCAGCGCAGTAGTAGTCAGCTTC O09ig197    PCA assembly of {?gliadin binding scFv?} (B09ig171) ACGACTCTCTGAACGTTTGGGTTTTCGGTGGTGGCACTAAACTGACCG O09ig198    PCA assembly of {?gliadin binding scFv?} (B09ig171) GACGGAGCAGCTTTCGGCTGACCCAGAACGGTCAGTTTAGTGCCACCA O09ig199    PCA assembly of {?gliadin binding scFv?} (B09ig171) CAGCCGAAAGCTGCTCCGTCTGTTACCCTGTTCCCGCCATCCTCTGCT O09ig200    PCA assembly of {?gliadin binding scFv?} (B09ig171) CGTTAGGATCCAGCAGCAGCAGAGGATGGCGGGAACA

Gene Synthesis of B09ig201 {<Cub>}
Pool O09ig201 through O09ig206, assemble by PCA PCR O09ig201/O09ig206 on PCA reaction       (164, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig201   {?Cub?}

O09ig201    PCA assembly of {?Cub?} (B09ig201) CCATAGAATTCATGAGATCTTCTGGTGGTATCCCGCCGG O09ig202    PCA assembly of {?Cub?} (B09ig201) TACCAGCGAAGATGAGGCGTTGCTGGTCCGGCGGGATACCACCAGAA O09ig203    PCA assembly of {?Cub?} (B09ig201) ACGCCTCATCTTCGCTGGTAAACAACTCGAAGACGGTCGTACCCTGT O09ig204    PCA assembly of {?Cub?} (B09ig201) TAGATTCTTTCTGAATATTGTAGTCAGACAGGGTACGACCGTCTTCG O09ig205    PCA assembly of {?Cub?} (B09ig201) CAATATTCAGAAAGAATCTACCCTCCATCTGGTTCTGCGTCTGCGTG O09ig206    PCA assembly of {?Cub?} (B09ig201) CGTTAGGATCCACCACCACGCAGACGCAGAACCAGA

Gene Synthesis of B09ig207 {<Nub>}
Pool O09ig207 through O09ig212, assemble by PCA PCR O09ig207/O09ig212 on PCA reaction       (173, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig207   {?Nub?}

O09ig207    PCA assembly of {?Nub?} (B09ig207) CCATAGAATTCATGAGATCTGGTCTGATGCAAATTTTCGTTAA O09ig208    PCA assembly of {?Nub?} (B09ig207) CAGGGTGCCAGTTTTGCCAGTCAGGGTTTTAACGAAAATTTGCATCAG O09ig209    PCA assembly of {?Nub?} (B09ig207) CTGGCAAAACTGGCACCCTGGAAGTTGAATCTTCTGACACCATCGACA O09ig210    PCA assembly of {?Nub?} (B09ig207) TCTTTGTCCTGGATTTTAGATTTAACGTTGTCGATGGTGTCAGAAGAT O09ig211    PCA assembly of {?Nub?} (B09ig207) TCTAAAATCCAGGACAAAGAAGGTATTCCAGGTGGTTGCCGTCCGCGT O09ig212    PCA assembly of {?Nub?} (B09ig207) CGTTAGGATCCCATAACACGCGGACGGCAACCACCTG

Gene Synthesis of B09ig137 {<Hia AtD>}
Pool O09ig137 through O09ig144, assemble by PCA PCR O09ig137/O09ig144 on PCA reaction       (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig137   {?Hia AtD?}

O09ig137    PCA assembly of {?Hia AtD?} (B09ig137) CCATAGAATTCATGAGATCTCTGCGTCGTGAAACCTCTGGTGGTGTTG O09ig138    PCA assembly of {?Hia AtD?} (B09ig137) CCTGCGGCAGGTTAGCGGTAGCGATAGCAGCAGCAACACCACCAGAGGTTTCA O09ig139    PCA assembly of {?Hia AtD?} (B09ig137) TACCGCTAACCTGCCGCAGGCTTACGTTCAGGGTCGTGGTATGACCTCTGTTG O09ig140    PCA assembly of {?Hia AtD?} (B09ig137) CGATAGCAGACTGACCCTGGTAAGAAGAAACACCAACAGAGGTCATACCACGA O09ig141    PCA assembly of {?Hia AtD?} (B09ig137) CCAGGGTCAGTCTGCTATCGCTGTTGGTGTTTCTGCTGTTTCTGAATCTGGTC O09ig142    PCA assembly of {?Hia AtD?} (B09ig137) TGTTAGCAGAACCAGAGAATTTGAAAACCCAGTGACCAGATTCAGAAACAGCA O09ig143    PCA assembly of {?Hia AtD?} (B09ig137) ATTCTCTGGTTCTGCTAACACCCGTTCTCACGTTGGTGTTGGTGCTGGTGTTG O09ig144    PCA assembly of {?Hia AtD?} (B09ig137) CGTTAGGATCCCCATTGATAGCCAACACCAGCACCAACACCA

Gene Synthesis of B09ig145 {<type IIIs Needle Complex scFV>}
Pool O09ig145 through O09ig170, assemble by PCA PCR O09ig145/O09ig170 on PCA reaction       (863, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig145   {?type IIIs Needle Complex scFV?}

O09ig145    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CCATAGAATTCATGAGATCTGCTGAACTGGTTATGACCCAGACCCCGT O09ig146    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) TAACAGTGCCACCAACAGCAGCGGAAACCGGAGACGGGGTCTGGGTCATAACC O09ig147    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) TGCTGTTGGTGGCACTGTTACCATCTCTTGCCAATCCTCTCAGTCTGTTTACA O09ig148    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) GTTTCTGCTGATACCAAGACAGCAGGTTGTTGTTGTAAACAGACTGAGAGGAT O09ig149    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) GTCTTGGTATCAGCAGAAACCGGGTCAGCCGCCGAAACTGCTGATCTACAACG O09ig150    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) AACGAGACGGAACACCAGAAGCCAGTTTAGAAGCGTTGTAGATCAGCAGTTTC O09ig151    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) TTCTGGTGTTCCGTCTCGTTTCAAAGGTTCTGGTTCTGGCACTCAGTTCACCC O09ig152    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CTGCGTCGTCGCATTCGAGATCGGAAATAGTCAGGGTGAACTGAGTGCCAGAA O09ig153    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) TCTCGAATGCGACGACGCAGCAACCTACTACTGCCAGGGCACTTACTACTCTT O09ig154    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) TAGTGCCACCACCGAAGGTCAGATACCAACCAGAAGAGTAGTAAGTGCCCTGG O09ig155    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) GACCTTCGGTGGTGGCACTAAACTGGACGTTAAAGGTCAGTCTGGTCAGTCTG O09ig156    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CAACACCCTGACCTTCACCAGAACCCGGTTTACCAGACTGACCAGACTGACCT O09ig157    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) TGGTGAAGGTCAGGGTGTTGGTTCTCAGCAACAACTCGTTGAATCTGGTGGTC O09ig158    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) GCAGGGTCAGCGGAGTGCCTGGAGTAACCAGACGACCACCAGATTCAACGAGT O09ig159    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) AGGCACTCCGCTGACCCTGCGTTGCACCGCTTCTGGTTTCTCTCTGTCTAACT O09ig160    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) TACCCGGAGCCTGACGAACCCAGTTCATGTAGTAGTTAGACAGAGAGAAACCA O09ig161    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) GGTTCGTCAGGCTCCGGGTAAAGGTCTGGAATGGATCGGTATCATCTACGAAT O09ig162    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CTTTAGCCCAAGAAGCGTAGTAGGTGAAACCAGATTCGTAGATGATACCGATC O09ig163    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CTACGCTTCTTGGGCTAAAGGTCGTTTCACCATCTCTAAAACCTCTACCACTA O09ig164    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CCTCGGTGGTTGGGGAGGTGATTTTCAGGTCCATAGTGGTAGAGGTTTTAGAG O09ig165    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CACCTCCCCAACCACCGAGGATACCGCTACCTACTTCTGCGCTCGTTTCGACA O09ig166    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CCCACGGGTCGAAAGCACCACCACGGTAACCGATGTCGAAACGAGCGCAGAAG O09ig167    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) TGGTGCTTTCGACCCGTGGGGTCCGGGCACTCTGGTTACCGTTTCTTCTGGTC O09ig168    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CAACGAGTTGAGAAACAGACGGAGCTTTCGGCTGACCAGAAGAAACGGTAACC O09ig169    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) GTCTGTTTCTCAACTCGTTGGTGCTGAACAAAAGCTCATCTCCGAAGAGGACC O09ig170    PCA assembly of {?type IIIs Needle Complex scFV?}  (B09ig145) CGTTAGGATCCAGCACCGTTCAGGTCCTCTTCGGAGATGAGC

Gene Synthesis of B09ig213 { }
Pool O09ig213 through O09ig238, assemble by PCA PCR O09ig213/O09ig238 on PCA reaction       (860, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig213   {?caspase 3?}

O09ig213    PCA assembly of {?caspase 3?} (B09ig213) CCATAGAATTCATGAGATCTGAAAACACCGAAAACTCTGTTGACT O09ig214    PCA assembly of {?caspase 3?} (B09ig213) TGATTTTCGGTTCCAGGTTTTTGATAGATTTAGAGTCAACAGAGTTTTCGGTG O09ig215    PCA assembly of {?caspase 3?} (B09ig213) AAACCTGGAACCGAAAATCATTCATGGTTCTGAATCTATGGACTCTGGTATCT O09ig216    PCA assembly of {?caspase 3?} (B09ig213) CCGGGTAGTCCATTTTGTAAGAGTTGTCCAGAGAGATACCAGAGTCCATAGAT O09ig217    PCA assembly of {?caspase 3?} (B09ig213) TTACAAAATGGACTACCCGGAAATGGGTCTGTGCATCATCATCAACAACAAAA O09ig218    PCA assembly of {?caspase 3?} (B09ig213) AACGAGAGGTCATGCCAGTGGACTTATGAAAGTTTTTGTTGTTGATGATGATG O09ig219    PCA assembly of {?caspase 3?} (B09ig213) CACTGGCATGACCTCTCGTTCTGGCACTGACGTTGACGCTGCTAACCTGCGTG O09ig220    PCA assembly of {?caspase 3?} (B09ig213) TACGAACTTCGTATTTCAGGTTACGGAAGGTTTCACGCAGGTTAGCAGCGTCA O09ig221    PCA assembly of {?caspase 3?} (B09ig213) CCTGAAATACGAAGTTCGTAACAAAAACGACCTGACCCGTGAAGAAATCGTTG O09ig222    PCA assembly of {?caspase 3?} (B09ig213) AGTGATCCTCTTTAGAAACGTCACGCATCAGTTCAACGATTTCTTCACGGGTC O09ig223    PCA assembly of {?caspase 3?} (B09ig213) CGTTTCTAAAGAGGATCACTCTAAACGTTCTTCTTTCGTTTGCGTTCTGCTGT O09ig224    PCA assembly of {?caspase 3?} (B09ig213) TAGTGCCGAAGATGATACCTTCTTCACCGTGAGACAGCAGAACGCAAACGAAA O09ig225    PCA assembly of {?caspase 3?} (B09ig213) AGGTATCATCTTCGGCACTAACGGTCCGGTTGACCTGAAAAAAATCACCAACT O09ig226    PCA assembly of {?caspase 3?} (B09ig213) CAGTCAGAGAACGGCAGCGATCGCCGCGAAAGAAGTTGGTGATTTTTTTCAGG O09ig227    PCA assembly of {?caspase 3?} (B09ig213) TCGCTGCCGTTCTCTGACTGGCAAACCAAAGCTCTTTATTATTCAGGCTTGTC O09ig228    PCA assembly of {?caspase 3?} (B09ig213) CGGTTTCGATACCGCAGTCCAGTTCAGTGCCGCGACAAGCCTGAATAATAAAG O09ig229    PCA assembly of {?caspase 3?} (B09ig213) GGACTGCGGTATCGAAACCGACTCTGGTGTTGACGACGACATGGCTTGCCACA O09ig230    PCA assembly of {?caspase 3?} (B09ig213) AAGCGTACAGGAAGTCAGCTTCAACCGGGATTTTGTGGCAAGCCATGTCGTCG O09ig231    PCA assembly of {?caspase 3?} (B09ig213) AGCTGACTTCCTGTACGCTTACTCTACCGCTCCGGGTTACTACTCTTGGCGTA O09ig232    PCA assembly of {?caspase 3?} (B09ig213) GGGATTGAATAAACCAAGAACCGTCTTTAGAGTTACGCCAAGAGTAGTAACCC O09ig233    PCA assembly of {?caspase 3?} (B09ig213) TTCTTGGTTTATTCAATCCCTCTGCGCTATGCTGAAACAGTACGCTGACAAAC O09ig234    PCA assembly of {?caspase 3?} (B09ig213) GGTTAACACGGGTCAGAATATGCATAAACTCCAGTTTGTCAGCGTACTGTTTC O09ig235    PCA assembly of {?caspase 3?} (B09ig213) TATTCTGACCCGTGTTAACCGTAAAGTTGCTACCGAGTTTGAATCTTTCTCTT O09ig236    PCA assembly of {?caspase 3?} (B09ig213) GGATCTGTTTTTTAGCGTGGAAGGTAGCGTCGAAAGAGAAAGATTCAAACTCG O09ig237    PCA assembly of {?caspase 3?} (B09ig213) CCACGCTAAAAAACAGATCCCGTGCATCGTTTCTATGCTGACCAAAGAACTGT O09ig238    PCA assembly of {?caspase 3?} (B09ig213) CGTTAGGATCCGTGGTAGAAGTACAGTTCTTTGGTCAGCATA

Gene Synthesis of B09ig239 {<mgfp-5>}
Pool O09ig239 through O09ig248, assemble by PCA PCR O09ig239/O09ig248 on PCA reaction       (260, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig239   {?mgfp-5?}

O09ig239    PCA assembly of {?mgfp-5?} (B09ig239) CCATAGAATTCATGAGATCTTCTTCCGAGGAATACA O09ig240    PCA assembly of {?mgfp-5?} (B09ig239) GGTGTTACCCGGGTAGTAACCACCTTTGTATTCCTCGGAAGAAGAT O09ig241    PCA assembly of {?mgfp-5?} (B09ig239) GTTACTACCCGGGTAACACCTACCACTACCACTCTGGTGGTTCTTA O09ig242    PCA assembly of {?mgfp-5?} (B09ig239) AACCACCGTGGTAACCAGAACCGTGGTAAGAACCACCAGAGTGGTA O09ig243    PCA assembly of {?mgfp-5?} (B09ig239) TTCTGGTTACCACGGTGGTTACAAAGGTAAATACTACGGTAAAGCT O09ig244    PCA assembly of {?mgfp-5?} (B09ig239) TTTTTGTATTTGTAGTAGTATTTTTTAGCTTTACCGTAGTATTTAC O09ig245    PCA assembly of {?mgfp-5?} (B09ig239) TACTACTACAAATACAAAAACTCTGGTAAATACAAATACCTGAAAA O09ig246    PCA assembly of {?mgfp-5?} (B09ig239) ACCTTTACGGTGGTATTTACGAGCTTTTTTCAGGTATTTGTATTTA O09ig247    PCA assembly of {?mgfp-5?} (B09ig239) GTAAATACCACCGTAAAGGTTACAAAAAATACTACGGTGGTGGTTC O09ig248    PCA assembly of {?mgfp-5?} (B09ig239) CGTTAGGATCCAGAAGAACCACCACCGTAGTATTT

Gene Synthesis of B09ig249 {<Tev N>}
Pool O09ig249 through O09ig260, assemble by PCA PCR O09ig249/O09ig260 on PCA reaction       (386, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig249   {?Tev N?}

O09ig249    PCA assembly of {?Tev N?} (B09ig249) CCATAGAATTCATGAGATCTGGTGAATCTCTGTTCAAAGGTCCG O09ig250    PCA assembly of {?Tev N?} (B09ig249) CAGATGGTAGAAGAGATCGGGTTGTAGTCACGCGGACCTTTGAACAGAGATT O09ig251    PCA assembly of {?Tev N?} (B09ig249) CCGATCTCTTCTACCATCTGCCACCTGACCAACGAATCTGACGGTCACACCA O09ig252    PCA assembly of {?Tev N?} (B09ig249) GAACGGACCGAAACCGATACCGTACAGAGAGGTGGTGTGACCGTCAGATTCG O09ig253    PCA assembly of {?Tev N?} (B09ig249) GTATCGGTTTCGGTCCGTTCATCATCACCAACAAACACCTGTTCCGTCGTAA O09ig254    PCA assembly of {?Tev N?} (B09ig249) CGTGGAGGGATTGAACCAGCAGAGTGCCGTTGTTACGACGGAACAGGTGTTT O09ig255    PCA assembly of {?Tev N?} (B09ig249) GCTGGTTCAATCCCTCCACGGTGTTTTCAAAGTTAAAAACACCACCACCCTC O09ig256    PCA assembly of {?Tev N?} (B09ig249) ATCATGTCACGACCGTCGATGAGATGTTGTTGGAGGGTGGTGGTGTTTTTAA O09ig257    PCA assembly of {?Tev N?} (B09ig249) ATCGACGGTCGTGACATGATTATTATTCGTATGCCAAAGGATTTTCCGCCGT O09ig258    PCA assembly of {?Tev N?} (B09ig249) CTGCGGTTCACGGAATTTGAGCTTTTGCGGGAACGGCGGAAAATCCTTTGGC O09ig259    PCA assembly of {?Tev N?} (B09ig249) TCAAATTCCGTGAACCGCAGCGTGAAGAACGTATCTGCCTCGTAACTACCAA O09ig260    PCA assembly of {?Tev N?} (B09ig249) CGTTAGGATCCGGTCTGGAAGTTGGTAGTTACGAGGCAGAT

Gene Synthesis of B09ig261 {<Tev C>}
Pool O09ig261 through O09ig276, assemble by PCA PCR O09ig261/O09ig276 on PCA reaction       (403, EcoRI/BamHI Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig261   {?Tev C?}

O09ig261    PCA assembly of {?Tev C?} (B09ig261) CCATAGAATTCATGAGATCTAAATCTATGTCCTCTATG O09ig262    PCA assembly of {?Tev C?} (B09ig261) GGAAGGTGCAAGAGGTGTCAGAAACCATAGAGGACATAGATTTAG O09ig263    PCA assembly of {?Tev C?} (B09ig261) TGACACCTCTTGCACCTTCCCATCCTCTGACGGTATCTTCTGGAA O09ig264    PCA assembly of {?Tev C?} (B09ig261) ACCGTCTTTGGTCTGAATCCAGTGTTTCCAGAAGATACCGTCAGA O09ig265    PCA assembly of {?Tev C?} (B09ig261) GGATTCAGACCAAAGACGGTCAGTGCGGTTCTCCGCTGGTTTCTA O09ig266    PCA assembly of {?Tev C?} (B09ig261) ATACCAACGATGAAACCGTCACGGGTAGAAACCAGCGGAGAACCG O09ig267    PCA assembly of {?Tev C?} (B09ig261) GACGGTTTCATCGTTGGTATCCACTCTGCTTCTAACTTCACCAAC O09ig268    PCA assembly of {?Tev C?} (B09ig261) GAACAGAGGTGAAGTAGTTGTTGGTGTTGGTGAAGTTAGAAGCAG O09ig269    PCA assembly of {?Tev C?} (B09ig261) CAACTACTTCACCTCTGTTCCAAAGAATTTTATGGAACTGCTGAC O09ig270    PCA assembly of {?Tev C?} (B09ig261) AACCCATTGTTGTGCTTCCTGGTTGGTCAGCAGTTCCATAAAATT O09ig271    PCA assembly of {?Tev C?} (B09ig261) AGGAAGCACAACAATGGGTTTCTGGTTGGCGTCTGAACGCTGACT O09ig272    PCA assembly of {?Tev C?} (B09ig261) ACTTTGTGACCACCCCACAGAACAGAGTCAGCGTTCAGACGCCAA O09ig273    PCA assembly of {?Tev C?} (B09ig261) CTGTGGGGTGGTCACAAAGTTTTCATGTCTAAACCGGAAGAACCG O09ig274    PCA assembly of {?Tev C?} (B09ig261) GGGTAGCTTCTTTAACCGGCTGGAACGGTTCTTCCGGTTTAGACA O09ig275    PCA assembly of {?Tev C?} (B09ig261) GCCGGTTAAAGAAGCTACCCAACTCATGTCTGAACTGGTTTACTC O09ig276    PCA assembly of {?Tev C?} (B09ig261) CGTTAGGATCCCTGAGAGTAAACCAGTTCAGACAT

PCR Construction of {<espP(beta)>}
PCR ca998/O09ig280 on M10044		 	(1362, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          	(EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09espP   		{<espP(beta)>}

ca998	Forward Oligo CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC O09ig280	Reverse Oligo for the removal of the stop codon from {<espP(beta)>} CGTTAGGATCCgaacgagtaacggaaattagcg

PCR Construction of {<upaG_short>}
PCR ca998/O09ig277 on M10026		 		(279, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          	(EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09upaG   		{<espP(beta)>}

ca998	Forward Oligo CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC O09ig277	Reverse Oligo for the removal of stop codon from {<upaG_short>} CGTTAGGATCCccactgaataccggcaccgagtgcg

PCR Construction of {<CPG_L2>}
PCR ca998/O09ig278 on M10086		 		(873, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          	(EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09cpgl2   		{<CPG_L2>}

ca998	Forward Oligo CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC O09ig278	Reverse Oligo for the removal of stop codon from {<CPG_L2>} CGTTAGGATCCcgcgctataatctaccggcccttcc

PCR Construction of {<CPG_L6>}
PCR ca998/O09ig279 on M10011		 		(873, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          	(EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09cpgl6  				{<CPG_L6>}

ca998	Forward Oligo CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC O09cpgl6	Reverse Oligo for the removal of stop codon from {<CPG_L6>} CGTTAGGATCCctgccagtcccagttactccagcg

PCR Construction of {<TshA>}
PCR ca998/O09ig283 on M10088	(1575, EcoRI/BamHI) Sub in pBca9523-Bca1144#5         	(EcoRI/BamHI, 2472+1224, L) Product is pBca9523-O09ig283   	{<TshA>} - Ca998 	forward oligo gtatcacgaggcagaatttcag O09ig283 	reverse oligo with stop codons removed for {<TshA>} gacggGGATCCgaatgaataacgaatattag

PCR Construction of {<eCPX>}
PCR ca998/O09ig282 on M10014 	(477, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          	(EcoRI/BamHI, 2472+1224, L) Product is pBca9523-O09ig282   	{<eCPX>} - Ca998 	forward oligo gtatcacgaggcagaatttcag O09ig282 	reverse oligo with stop codons removed for {<eCPX>} ccggtGGATCCAGAGCTTGCAGTACGGCTTT

PCR Construction of {<ehaB>}
PCR ca998/O09ig281 on M10052	(1229, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          	(EcoRI/BamHI, 2472+1224, L) Product is pBca9523-O09ig281   	{<ehaB>} - Ca998 	forward oligo gtatcacgaggcagaatttcag O09ig281 	reverse oligo with stop codons removed for {<ehaB>} cggtaGGATCCccaggtatatttaacaccca

SOEing construction of B09ig302 {<VirG AtD>}
PCR O09ig302/O09ig301 on S. Flexneri (490bp, gp = frag1) PCR O09ig300/O09ig303 on S. Flexneri      (986bp, gp = frag2)

PCR O09ig302/O09ig303 on mixed frags   (1457bp, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig302         {?VirG AtD?}

O09ig302   Forward PCR of {?VirG AtD?} (B09ig302) AAGTGGAATTCATGAGATCTTCGTCAAATGTCGGTCAGAC O09ig301   Reverse internal oligo for {?VirG AtD?} (B09ig302) CTGCCAGCCTCAGGGCGCC O09ig300   Forward internal oligo for {?VirG AtD?} (B09ig302) GGCGCCCTGAGGCTGGCAG O09ig303   Reverse PCR of {?VirG AtD?} (B09ig302) GTTAGGGATCCGAAGGTATATTTCACACCC

SOEing construction of B09ig306 {<yuaQ AtD>}
PCR O09ig306/O09ig305 on MG1655(ask chris about EcoRV before start) (464bp, gp = frag1) PCR O09ig304/O09ig307 on MG1655(ask chris about EcoRV before start) (452bp, gp = frag2)

PCR O09ig306/O09ig307 on mixed frags   (898bp, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig306         {?yuaQ AtD?}

O09ig306   Forward PCR of {?yuaQ AtD?}  (B09ig306) AAGTGGAATTCATGAGATCTGGAGAACCGAAAGCAACCAG O09ig305   Reverse internal oligo for {?yuaQ AtD?}  (B09ig306) GCTGCTGAACTCCCCGATTTTC O09ig304   Forward internal oligo for {?yuaQ AtD?}  (B09ig306) GAAAATCGGGGAGTTCAGCAGC O09ig307   Reverse PCR of {?yuaQ AtD?}  (B09ig306) GTTAGGGATCCGAATGTATACTTCACACCCAG

SOEing construction of B09ig310 {<AIDA-1 AtD>}
PCR O09ig310/O09ig309 on EHEC (1184bp, gp = frag1) PCR O09ig308/O09ig311 on EHEC (287bp, gp = frag2)

PCR O09ig310/O09ig311 on mixed frags   (1450bp, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig310         {?AIDA-1 AtD?}

O09ig310   Forward PCR of {?AIDA-1 AtD?} (B09ig310) AAGTGGAATTCATGAGATCTGCTGGCAATGTGTTAGTGGTG O09ig309   Reverse internal oligo for {?AIDA-1 AtD?} (B09ig310) ATGGTGGCTCTTGAGCCAGGTTTT O09ig308   Forward internal oligo for {?AIDA-1 AtD?} (B09ig310) AAAACCTGGCTCAAGAGCCACCAT O09ig311   Reverse PCR of {?AIDA-1 AtD?} (B09ig310) GTTAGGGATCCGAATTGCCACTTAATGCCAAC

SOEing construction of B09ig314 {<cel3A!}
PCR O09ig314/O09ig313 on TEMPLATE      (2413bp, gp = frag1) PCR O09ig312/O09ig315 on TEMPLATE      (171bp, gp = frag2)

PCR O09ig314/O09ig315 on mixed frags   (2566bp, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig314         {?cel3A!}

O09ig314   Forward PCR of {?cel3A!} (B09ig314) AAGTGGAATTCATGAGATCTGGTTGTGATTCCCGCGCTCC O09ig313   Reverse internal oligo for {?cel3A!} (B09ig314) GCGCCGGAGTTCGGGTAG O09ig312   Forward internal oligo for {?cel3A!} (B09ig314) CTACCCGAACTCCGGCGC O09ig315   Reverse PCR of {?cel3A!} (B09ig314) GTTAGGGATCCGGGGCAGGCGACGTCTTTTC

SOEing construction of B09ig318 {<cel5B!}
PCR O09ig318/O09ig317 on TEMPLATE      (1226bp, gp = frag1) PCR O09ig316/O09ig319 on TEMPLATE      (418bp, gp = frag2)

PCR O09ig318/O09ig319 on mixed frags   (1625bp, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig318         {?cel5B!}

O09ig318   Forward PCR of {?cel5B!} (B09ig318) AAGTGGAATTCATGAGATCTGATGTCGCGCCGCTGTCCGTAC O09ig317   Reverse internal oligo for {?cel5B!} (B09ig318) CAGGCTGGACGAGCTGGAG O09ig316   Forward internal oligo for {?cel5B!} (B09ig318) CTCCAGCTCGTCCAGCCTG O09ig319   Reverse PCR of {?cel5B!} (B09ig318) GTTAGGGATCCATTGTTACAAACATTACCGC

SOEing construction of B09ig322 {<cel9A!}
PCR O09ig322/O09ig321 on TEMPLATE      (1055bp, gp = frag1) PCR O09ig320/O09ig323 on TEMPLATE      (1784bp, gp = frag2)

PCR O09ig322/O09ig323 on mixed frags   (2821bp, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig322         {?cel9A!}

O09ig322   Forward PCR of {?cel9A!} (B09ig322) AAGTGGAATTCATGAGATCTGAGGTGGGTAACCCCCGTGTC O09ig321   Reverse internal oligo for {?cel9A!} (B09ig322) CCAATAGAACTCATCGGC O09ig320   Forward internal oligo for {?cel9A!} (B09ig322) GCCGATGAGTTCTATTGG O09ig323   Reverse PCR of {?cel9A!} (B09ig322) GTTAGGGATCCCTGACATACACTTCCGGTTAC

Basic PCR construction of B09ig324 {<SOD>}
PCR O09ig324/O09ig325 on MG1655 (ask chris abuot EcoRV before start) (493, EcoRI/BamHI Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig324         {?SOD?}

O09ig324   Forward PCR of {?SOD?} (B09ig324) CCATAGAATTCATGAGATCTGCCAGTGAAAAAGTCGAGATG O09ig325   Reverse PCR of {?SOD?} (B09ig324) CGTTAGGATCCCTTAATTACACCACAGGCATAG

Basic PCR construction of B09ig326 {<Tir-M>}
PCR O09ig326/O09ig327 on E2348/69(O127:H6)EPEC (352, EcoRI/BamHI) Sub in pBca9523-Bca1144#5          (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig326         {?Tir-M?}

O09ig326   Forward PCR of {?Tir-M?} (B09ig326) CCATAGAATTCATGAGATCTCAGGCGTTGGCTTTGACACC O09ig327   Reverse PCR of {?Tir-M?} (B09ig326) CGTTAGGATCCTGAAAGCTGTAATTCCTCCTG

Basic PCR construction of B09ig328 {<cel6A!}
PCR O09ig328/O09ig329 on TEMPLATE      (2041, EcoRI/BamHI Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig328         {?cel6A!}

O09ig328   Forward PCR of {?cel6A!} (B09ig328) CCATAGAATTCATGAGATCTGCATGTACCTACAAAATTAC O09ig329   Reverse PCR of {?cel6A!} (B09ig328) CGTTAGGATCCTTGCAGTGGTGGATAAGCG