IGEM:Peking/2007/Switch-Notebook/2007-8-27

mini-prep by kit
lacIq-pcc009, RS1, SD1, RD2

digestion test
RS1, SD1, RD2 (template 3uL, SalI 0.5uL, buffer H 2uL, ddH2O 14.5uL) T1Te-pMD18T (template 5uL, EcoRI/NdeI 0.25uL, buffer H 2uL, ddH2O 12.5uL) lacIq-pcc009 (template 5uL, SalI/XhoI 0.25uL, buffer H 2uL, ddH2O 12.5uL)

precipitation of lacIq and pcc009 after digestion
failed! no visible bands!

digestion test
lacIq-pcc009 (BSA/Trition X-100/buffer T 2uL, template 5uL, KpnI/SacII 0.25uL, ddH2O 8.5uL)



double digestion of T1Te-pMD18T and T1T2-pcc010
plasmid/fragment 30/3uL buffer H 10uL ClaI/SalI 2uL ddH2O 56/83uL overnight in 37 degree



colony PCR of lacI