IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/16

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Thursday 16th September

 * Ligations were set up between the promoter-GFP and the digested pSB1C3 plasmid (which was retrieved from part BBa_J04450) using the method shown bellow:


 * These ligations were left for 2 hours then using 5μL they were transformed into chemically competent DH5 alpha cells.


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