User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/23

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05/23/11

 * &#x2713; Miniprep digests: KAH182(4)
 * &#x2713; Assemblies: KAH183, 184

Minipreps > Check with E/P digests

Assemblies
 * 1) KAH183: FC7/(E/S)/708 &#x2713; + (1) KAH182/(E/X)/4869
 * 2) KAH184: HPK/(E/S)/516 &#x2713; + "

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

---> Add 11 μL to 30 μL DH5α Turbo


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