IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/10

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Team Allergy
ihpRNA
 * Only 2 colonies grew up (V10 9 (37c1) and V10 12 B(38 c2)) ger ihprna constructs
 * growing up colonies again in 5 mL cultures w/less antibiotic (50 ng/uL of kan)
 * concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
 * Diagnostic Digest (Should see ~ 7 kb band and 840 band)
 * Did not see exepcted bands (waiting on cultures grown up today to miniprep and hopefully transform into agrobacterium tommorow)

amiRNA
 * Ligated into V9/V10 and transformed

Team Fence



 * ladder
 * PhyB (backbone)
 * Barstar 4 (insert)
 * V26 (backbone)
 * GalDBD1 (insert)
 * GalDBD5 (insert)
 * B21 (backbone)

Digests 8/10

 * PhyB with Bam and Nco
 * Barstar with Pst and Eco
 * V26 with Pst and Eco
 * Gal4DBD with Eco and Spe (Gal4DBD minipreps 1 and 5)
 * Arp2BB PCR product with Xba and Pst
 * Arp PCR product with Xba and Pst
 * B21 with Xba and Pst
 * ACC synthase with Eco and Spe
 * Barnase ligation product with Eco and Xba


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