IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/12

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8/12/10
No growth on any of yesterday's plates.

3:10 PM

Electroporation

100 μL cells, 3 μL ligation product (pRpoS438 2). electroporate, add 1 mL SOC, recover at 37°C for an hour.

3:20 PM

Heat Shock Transformation

50 μL cells, 2 μL ligation in a tube

10 min on ice

45 sec at 45°C

2 min on ice

add 1 mL SOC, recover for 1 hour at 37°C.

Samples:

pRpoS438 2

pArsRF1R2 2'

pArsRF1R2 1

pArsRF1R2 2

4:15 PM

Plating

Plated 200 μL pRpoS438 2 electroporation product on CAP plate

4:35 PM

Plating

Plated 300 μL of heat shocked pRpoS438 2, pArsRF1R2 2', pArsRF1R2 1, pArsRF1R2 2, on CAP plates.


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