Imperial College/Courses/Spring2008/Synthetic Biology/Part Measurement and Characterisation/Resources

 Wet Lab: Part Measurements and Characterisation

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Calibrated Measurements and Calibration Curves:
 * OD600 versus viable cell counting (serial plate dilution)
 * Fluorescence measured versus purified protein concentration.
 * Fluoresence measured 'In Vivo' versus Fluorescence measured 'In Vitro' (happy cells and lysed cells)
 * Detailed protocols to be given: plate reader data analysis, [[
 * Collect data, and document their work in their OWW lab notebook.
 * Useful Protocols:
 * GFP calibration protocol(iGEM 2007)
 * GFP Calibration (Endy's Lab)
 * Absorbance

Promoter Characterisation using a standardised measurement kit
 * Each group is given X promoters to sort out by strength.
 * Detailed protocol to be given.
 * Collect data, and document their work in their OWW lab notebook.
 * BioBrick candidates:
 * I20259 : Constitutive_Promoter_1 (from combinatorial library) --GFPmut3--XXX
 * I20260 : Constitutive_Promoter_2 (from combinatorial library) --GFPmut3--XXX
 * I20261 : Constitutive_Promoter_3 (from combinatorial library) --GFPmut3--XXX
 * I7101 : pTetR--GFPmut3--XXX
 * I7107 : pLacI--GFPmut3--XXX
 * I7109 pCI--GFPmut3--XXX
 * Useful protocols:
 * Measurement-kit info
 * Instructions
 * Plate reader protocol

Inducible Promoter Characterisation
 * Using the previous protocols, students are expected to define their own standard protocol.
 * Collect data and document their work in their OWW lab notebook.
 * Model expression system, Analyze Data.
 * BioBrick candidates:
 * I0500 : pBAD promoter (only)
 * I13520 : pBAD--mRFP--XXX
 * I6058 : pBAD--EYFP--XXX
 * I13540 : pBAD--GFPmut3--XXX
 * Useful Protocols:
 * F2620 protocol

Inverter Characterisation
 * From the previous protocols students have to define their own standard protocol.
 * Collect data and document their work in their OWW lab notebook.
 * Model expression system, and Analyze Data.
 * BioBrick candidates:
 * J45996 : Exponential-Phase-Sensitive GFP Generator
 * J45995 : Stationary-Phase-Sensitive GFP Generator
 * J5517 : pBAD--LacIts--XXX--pLacI--GFP--XXX
 * E0611 : pBAD--tetR-Inverter--EYFP--XXX
 * E0610 : pBAD--LacI-Inverter--EYFP--XXX
 * E0612 : pBAD--cI-Inverter--EYFP--XXX
 * pSB1A10 : Screening plasmid (GFP-inputs/RFP-outputs)+ TetR-Inverter
 * Useful Protocols:
 * Inverter Protocol (Endy's Lab)

<font face="trebuchet ms" style="color:#FFFFFF" size="3">Resources on OpenWetWare:
 * Standardized_GFP_quantification
 * Measurement_kit
 * Absorbance measurement
 * PoPS and RiPS

<font face="trebuchet ms" style="color:#FFFFFF" size="3">Resources on the web