IGEM:IMPERIAL/2007/Notebook/2007-9-19

name=iGEM:IMPERIAL/2007/Notebook date=2007/09/20 view=threemonths format=%name/%year-%month-%day weekstart=7

Purification of LuxR-His

 * Today we innoculated 2 litres of LB medium with E.coli containing the luxR-his plasmid (pMU117) to be grown to an O.D.600 0.5. After this the cells were harvested and frozen overnight.
 * In addition an SDS-PAGE gel was run from a small sample of the soluble proeins from these cells, that had been purified using an affinity column (Nickel column for His tag).
 * The gel was left to destain overnight