Template:SBB-Protocols Enz6

Set up the following 10uL reaction in a PCR tube: 4uL ddH2O 4uL Miniprepped plasmid 1uL 10x NEB Buffer 2 .5uL EcoRI .5uL BamHI (for parts >250bp) or XhoI (for parts <250bp) FOR Righty Methylated Parts use Eco/Xho!


 * Incubate at 37 on the thermocycler for 30 minutes
 * Run an analytical gel
 * Take a picture of the gel
 * Calculate the expected fragment sizes
 * Are the calculated sizes consistent with the bands on the gel?