IGEM:MIT/2005/Future Experiments

Pathway ToxR

 * Test Expression of ToxR
 * Biobrick ToxR gene
 * Tag ToxR w/ antibody recognition/GFP
 * Test for presence of ToxR w/ FACs
 * Test Function of Pathway of ToxR
 * Biobrick ctx promoter
 * Suffix insertion of GFP into ctx promoter plasmid
 * Test pH/temperature changes for presence of GFP
 * Alternative? E. coli not sensitive to temp/pH changes?
 * Test Fusion of ToxR
 * [Marker][ToxR][Transmembrane Protein][Marker]
 * Test for first marker
 * Test for second marker
 * If both present, entire gene is produced!

Pathway FecA

 * Isolate FecA transduction gene sequence
 * Find coding sequences for FecR, FecI, Rpo, TonB, Exb D, Exb B, GFP, and fec promoter
 * Biobrick
 * Screen a linker that work to link FecA and antibody
 * Fuse antibody with FecA w/ diff. linkers to antibody
 * Screen for signal