User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/30

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05/30/11

 * &#x2713; Miniprep digests: check miR sensor set (re-transformed)

Minipreps > Check with E/P digests

-> Note: all worked except for FC1, KAH118, KAH119, KAH124. Use retransformations (#1, 2, 12, 13, 14, 15, 22) to grow 5mL cultures and try again. --> Result: Miniprep digests do not look good for FC1, KAH118, KAH119, but spec readings show ~160 ng/μL. Try one of each for transfection to see what happens. -> Spec readings show good concentrations for all others. Keep for U2OS transfectons. -> Made streak plate from 2 mL cultures yesterday.


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