User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/18

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Statistical analysis
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Short summary

Method

 * Students T-test on all ELISA's done so far
 * Ht31/Ht31P done separately from Ht31/S0
 * Student T-test, 2-tailed, α = 0.025

Conclusion

 * CSE+S0 has a too large Standard Deviation
 * A higher n is required
 * More experiments will be started
 * PKA is not significantly inhibited by Ht31
 * Increase Ht31
 * Perform VASP in increasing Ht31 concentrations
 * Epac does not effect IL-8 production
 * PKA decreases IL-8 production

Run 1: Ht31/Ht31P D9/D12

 * Counting cells 12Feb2010
 * Putting to S0 15Feb2010
 * Stimulating cells 16Feb2010
 * Ending stimulation & Viability 17Feb2010
 * ELISA 18Feb2010

Run 2: Ht31 Dose/Effect Curve & S0 D9/D12

 * Counting cells 19Feb2010
 * Putting to S0 23Feb2010
 * Stimulating cells 24Feb2010
 * Ending stimulation & Viability 25Feb2010
 * ELISA 26Feb2010

Run 3: Ht31/S0

 * Counting cells
 * 26Feb2010 D9/D12
 * 03March2010 D9 (P21)/D12 (P25)
 * Putting to S0
 * 03March D9/D12
 * 08March2010 D12 (P25)
 * 09March2010 D9 (P21)
 * Stimulating cells
 * 04March2010 D9 (samples lost)/D12
 * 09March2010 D12 (P25)
 * 10March2010 D9 (P21)
 * Ending stimulation & Viability
 * 05March2010 D12
 * 10March2010 D12 (P25)
 * 11March2010 D9 (P21)
 * ELISA
 * 16March2010 D12, D12 (P25) & D9 (P21)


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