User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/09

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Isolate Protein/RNA Jurkat cells, Codes IF
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Isolate proteins and RNA from Jurkat cells (P7)
 * Centrifuge 5 min. 300xg to collect the cells
 * Instead of 0.2 mL/mL TRIzol; 400 μL for the 6 mL TRIzol
 * 2 mL of isopropanol (instead of 0.5 mL/mL TRIzol

Attachment
   [[Media:IF_09082010_D9_P29.xls| IF D9 P29]]

   [[Media:09082010_jurkat_rnapdf.pdf| RNA concentration Jurkat cells]] <!-- ##### DO NOT edit below this line unless you know what you are doing. #####
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