ISISBio:Internal/Group meetings/13-June-08

Safety Issues:
 * Autoclave is currently being quoted for plumbing in which seems to be getting out of hand from a cost perspective.
 * Up to two days disruption to fit tank in ceiling
 * Query over status of bunsen burner, some suggestion that it needs to be protected and clamped down. This isn't really practical.
 * Use of chloroform to clean troughs
 * Action LW to ask for risk assessment for trough use

Current Projects Status:
 * Preparation of protein for QENS
 * MT - lots of GFP available, needs to be purified by gel filtration in advance of beamtime
 * Need to not spend too much more time on this
 * Action CN to confirm with MT when the beamtime is, prefer September
 * Preparation of mellitin for VGS
 * Currently checking to see whether initial plasmid has been made
 * Several more mol biol steps to go
 * Action LW to follow through with next steps
 * OmpF for SAH
 * LC has purified significant quantities of OmpF, total of about 6 mg
 * Played around with making vesicles. PE doesn't make very good vesicles so need to move 25:25:50 PE:PG:PC vesicles
 * Going to Oak Ridge for two weeks from 29 June -9 July to deuterate OmpF for SANS experiments
 * May need to 5mL DEAE column to Oak ridge for final wash stage
 * External reflection IR and DLS organised to do at Reading
 * Ion channels
 * Currently trying to purify MLok1 in large quantities for ILL on 19 July
 * Recent issues with funny bands on gels which seemed to appear in last two weeks. Checking batches against each other.
 * Some sample to take but possibly not as much as we want. Would like 3mg/mL for 5/6 contrasts.
 * Will also take some mthK to ILL to see whether can see change on addition of Ca2+
 * Fluorescent labelling
 * Think Michael had made pcr product,now need to purify and ligate to make plasmid

Next week

 * Luke using big FPLC all week
 * CN/Maria to use baby Akta end of week (Wed-Fri) probably
 * MS preparing and transforming Mlok1-LPETGG plasmid and recloning sortase
 * VGS probably continuing with mol biol

Week after

 * Dave Barlow/Damian experiment to express and purify GlpF/DCytB
 * Action CN to contact DB and suggest moving schedule up to avoid Thursday

Logistics

 * Need emergency protocol for MCR
 * Booking system for lab and equipment