User:Daniel Goodman/Notebook/Weiss/2010/01/19

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Restriction and Gel of PhyB917 & PIF3
Samples First 3 PhyB917 Samples (37 deg C, 10 min) PIF 3 Sample (65 deg C, 10 min)

Made 75 ml agar gel:
 * 75ml TAE
 * .75 g Agarose
 * 7.5 ul SYBR-SAFE

Put SYBR after cooling, cooled in water bath, set gel ~20 min.

Note: forgot to swirl after adding sybr, though color looked uniform

Cut top of gel off, saved bottom in 4°C fridge in ziploc w/ name&date

Loading Gel:
* One PIF3 and PhyB917 Samples 1-3 are new, the other PIF3 and 4-7 are recovered from freezer from Dec, may fail or be labelled incorrectly

Expected for PhyB917:
Cut with BstBI - two bands, 3579 and 5314

Expected for PIF3:
Cut with AvrII - two bands, 2164 and 4791