User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/03

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12/03/09

 * &#x2713; Minipreps: KAH105(2), 106(2), 107(2), 108(2)
 * &#x2713; Cultures: KAH93/MV2(2), 94/MV2(2), 95/MV2(2)
 * &#x2713; Assemblies: KAH99, 100, 101, 105, 106, 107, 108 into MV2

Minipreps > Check with E/P/ScaI triple digests

Assemblies
 * 1) KAH99/MV2: (1) KAH99/(E/P)/2913 + MV2/(E/P)/4529 &#x2713;
 * 2) KAH100/MV2: (2) KAH100/(E/P)/3085 + "
 * 3) KAH101/MV2: (3) KAH101/(E/P)/3157 + "
 * 4) KAH105/MV2: (4) KAH105/(E/P)/2794 + "
 * 5) KAH106/MV2: (5) KAH106/(E/P)/2788 + "
 * 6) KAH107/MV2: (6) KAH107/(E/P)/2966 + "
 * 7) KAH108/MV2: (7) KAH108/(E/P)/2960 + "

> Digests (Fermentas FD) --> E/P/ApaLI for KAH99-101 --> E/P/ScaI for KAH105-108

> Measure conc.'s

> Ligations


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