IGEM:IMPERIAL/2006/LabCalendar/8aug06

Lab Update 8th August
This Report is a summary for full details see the lab notebook

Aims For Friday

 * Finish Stage three Ligations (With the exception of the aiiA part)


 * Finish Stage two ligations (by wedensday)


 * Test part T9002 (This part is the AHL/GFP assay and the Predator AHL sensor)

Aims For today

 * Order Supplies John SY
 * Maxiprep and electroporate for ligations Deepti, Farah, Johhny and John SY
 * Test part T9002 Tom
 * PCR Cre Lox part John C

Current Ligation Status

 * Tick = Done and tested
 * ?   = Ligated and untested
 * X   = Done and Failed we will retry

Issues

Riboswitch


 * Unable to tell if worked from PCR
 * Will be sequenced (takes 2-3 days)

PCR


 * The PCR results do not correlate with the miniprep results

minipreps


 * These are not conclusive for many parts, we are looking into using a restriction digest

John Chattaway (Lab Co-ordinator)