IGEM:IMPERIAL/2006/LabCalendar/2006-8-12

Minipreps to check ligations of J37015 and J37016
The following parts were mini-preped:
 * L1, L2, L3, L4
 * M1, M2, M3, M4
 * R1, R2, R3, R4
 * V1, V2, V3, V4
 * Z1, Z3, Z4, Z5

Below is the one of the gels from the miniprep: The bands run (from left to right)
 * M1, M2, DNA ladder, R4, R3, R2, R1



Below is the second minprep gel containing (from left to right) :
 * Z5, Z4, Z3, Z1, DNA ladder, V4, V3, V2, V1, L4, L3, L2, L1







Problems


 * Gels overran so no conclusive evidence could be obtained
 * There were unexpected bands
 * This could be due to the fact that the cultures being minipreped were old
 * Could not run M3, M4 since we had no more enzyme for the digest left

Actions


 * Re-cultured the colonies which appear to have worked for re-minipreping these were
 * J37015
 * L 2,3,4
 * M 1,2
 * J37016
 * V 2,3,4
 * R 3,4

These have to be minipreped tomorrow and then digests have to be done on Monday

Maxiprep of 2H
successful

Ligation

 * Ligation of 12D->2H and electroporated
 * Cultured on LB Amp Plates and grown overnight

Gene Clean & Electroporation

 * Cre 1, 2, 3, 4
 * AiiA
 * AiiA, Cre 1, 2, and 4 were electroporated, cultured on LB Amp plates and grown overnight
 * Cre 3 sparked on electroporation, did not re-attempt since we already have three