User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/24

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Immunoblot repeat
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * To check if really nothing could be detected the same antibodies were used ON, repeat of yesterday.

Results

 * Immunoblot

Discussion

 * RIIα detection went fine now, no explanation why it didn't work before like the rest
 * The other antibodies still could not find anything
 * To see if it is due to the transfer to the membrane a new gel will be run and stained (Coomassie or Silver)
 * Another possibility is to do the RII overlay on the remaining blot of 22June2010 to see if there is anything that was precipitated together with RII subunits
 * As a positive control for AKAP95 and AKAP450 HeLa cells have been requested


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