User:Anthony Salvagno/Notebook/Research/2009/05/05/Gels

Lanes:
 * 1) Marker
 * 2) SapD (Steve Koch: Incomplete digestion?)
 * 3) Sap14
 * 4) Sap16
 * 5) Sap21
 * 6) SappBS (Steve Koch: Incomplete digestion?)
 * 7) NotpBS (Steve Koch: Same clone as previous lane, but complete digestion)
 * 8) NotD (Steve Koch: See lane 2; this is complete digestion with NotI as opposed to SapI)
 * 9) Not2
 * 10) Not16
 * 11) Not21
 * 12) Empty
 * 13) Saplib
 * 14) Notlib
 * 15) Empty
 * 16) Anchor

I have been naming tubes weirdly because I've been running into repetitive names. Here I have Sap# and Not# where number is the tube of which there is plasmid plus insert from a given tube (going way back) and Sap/Not is the restriction enzyme that I used to cut said tube. Sap and Notlib are libraries (after a bombing) of gDNA. See these pages:
 * User:Anthony Salvagno/Notebook/Research/2009/05/01/pAS lib for library explanation (Steve Koch:I'm guessing it's double-digested EcoRI/XhoI genomic DNA, but I don't think the information was written down).
 * User:Anthony Salvagno/Notebook/Research/2009/04/30/SapI Digestion of random fragments SapI digestions (lanes 2-5; not lane 6 though?)
 * explanation of naming conventions
 * User:Anthony Salvagno/Notebook/Research/2009/04/16/Gel Results Original shotgun clone analysis.

Lanes (inverted from picture): HP is the hairpin adapter. So this gel didn't work well. I had to run this in a vertical acrylomide gel. I'll have to write the protocol up later.
 * 1) Marker (50bp ladder)
 * 2) Marker (50bp ladder)
 * 3) NotHP
 * HP