User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/04/22

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Turn [FM7Ag/Y; FRT42D/CyoAg] stocks for experimental and control crosses.
 * Dissect ~30 gfp-h2au into 3 separate vials.
 * Potentially 1:10, 1:50, 1:100. Dissect ~30 flies. 10 per concentration.
 * Fix 30' in 4% Formaldehyde, PBS-T at RT.
 * Wash 4x5' in PBS-T at RT
 * Block in 2% NGS O/N


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