IGEM:IMPERIAL/2006/LabCalendar/2006-9-9

 TO DO: *Maxi & mini of new J37024 (1,2,3,4) and J37019 *Ligations & Electroporations: J37025 & J37022 *Testing:J37015 *Culture: J37015, J37019 and J37016 for J37015 testing

Testing J37015
Issues
 * Fresh cultures of 015, 019 & 016 failed to be made yesterday so testing is being conducted from the 10ml culture of 015 & 019 from the 8/9/06 and the remainder of the 2ml culture from 7/9/06.


 * OD600 of J37015: 2.0     reculture 0.5ml into 10ml
 * OD600 of J37019: 1.864   reculture 0.536ml into 10ml
 * OD600 of J37016: 2.0     reculture 0.8ml into 16ml


 * Placed in the shaker at 10:40, take out at 12:40.

Ligations

 * Maxied new J37024 (1,2,3,4) and J37019
 * Ligated J37025 & J37022 and electroporated