User:Maximilian Peters/Stock solutions

Bradykinin
Molecular weight: 1060.21 g/mol Concentration stock solution: 0.5 mM, 1 mg per 1.8 mL H2O Usual concentration for experiments: 0.1 μM Stability: 3-6 months Storage: -20°C Suppliers: Notes: Usual sold as acetate salt, molecular weight for anhydrous molecule with 100% purity, Sigma sells 95.5% purity with unknown amount of acetate, therefore concentration not 100% accurate

Cytosine arabinoside
Molecular weight: 279.68 g/mol Concentration stock solution: 1 mM, 28 mg per 100 mL or 25 mg (one vial) in 89.4 ml Stability: ? Storage: -20°C Suppliers: Sigma Incompatibilities: Notes: C6645 Notes: 300 μl aliquots

DNAse
Concentration stock solution: 1 mg per 50 μL H2O Stability: 2-4 weeks Storage: -20°C Suppliers: Incompatibilities: Notes: 50 μl aliquots

Glutamine
Molecular weight: 146.14 g/mol Concentration stock solution: 0.2 M, 29.228 g per 1000 mL Usual concentration for experiments: Stability: ? Storage: -20°C Suppliers: Sigma Incompatibilities: Notes: 1 ml aliquots

Insulin
Concentration stock solution: 12.5 mg per 1 ml 10 mM HCl Usual concentration for experiments: μM Stability: ? Storage: -20°C Suppliers: Sigma, I5500 Incompatibilities: Notes: 200 μl aliqouts

Lanthanum chloride
Molecular weight: 371.37 g/mol (*7H2O) Concentration stock solution: 0.5M, 186 mg per 1000 mL H2O Usual concentration for experiments: mM to μM Stability: >3 months Storage: +4°C Suppliers: Incompatibilities: precipitates with carbonates (buffer!), EGTA and BAPTA have higher affinity to La3+ than to Ca2+ Notes:

Nifedepine
Molecular weight: 346.335 g/mol Concentration stock solution: 25 mM, 8.66 mg per 1000 μL DMSO Usual concentration for experiments: μM Stability: 2-4 weeks Storage: -20°C Suppliers: Notes: N7634 Sigma, very light sensitive, protect solutions from excessive light, preferable fresh stock each day

Probenicid
Molecular weight: 285.36 g/mol Concentration stock solution: 250 mM, 71.25 mg per 1 mL of buffer (e.g. HBBS) Usual concentration for experiments: 1-2.5 mM Stability: <= 6 months Storage: -20°C Suppliers: Sigma, Invitrogen, ABCR Incompatibilities: Notes: Invitrogen sells Probenicid (water soluble) which seems to be (according to molecular weight) simply the sodium salt http://probes.invitrogen.com/media/pis/mp36400.pdf Dissolve the poweder in 90-95% of the calculated amount of buffer and add 10M NaOH to the desired volume

SKF-96365
Molecular weight: Concentration stock solution: 50 mM in H2O, 20.15 mg per 1000 μL Usual concentration for experiments: 1-50 μM Stability: at least 1 month Storage: -20°C Suppliers: Notes:

Trypsin
Concentration stock solution: 10 mg/200 μL H2O Usual concentration for experiments: μM Stability: 2-4 weeks Storage: -20°C Suppliers: Incompatibilities: Notes: 200 μL aliquots

Template
Molecular weight: g/mol Concentration stock solution: mM, 1 mg per 1.8 mL Usual concentration for experiments: μM Stability: 2-4 weeks Storage: -20°C Suppliers: Incompatibilities: Notes: