User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/01

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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12/01/09

 * &#x2713; Cultures: KAH011(8)
 * &#x2713; Assemblies: KAH93, 94, 95 into pcDNA3.1+puro/ΔpCMV(MV2), use E/P; KAH105, 106, 107, 108

Assemblies
 * 1) KAH93/MV2: (1) KAH93/(E/P)/4839 + (4) pcDNA3.1+puro/ΔCMV(MV2)/(E/P)/4529
 * 2) KAH94/MV2: (2) KAH94/(E/P)/5011 + "
 * 3) KAH95/MV2: (3) KAH95/(E/P)/5083 + "
 * 4) KAH105: (5) Ubc-Kstop(KAH102)/(E/S)/374 + Gal4DB-PEST-2xmCh-NLS-stop(KAH68)/(E/X)/5614 &#x2713;
 * 5) KAH106: (6) Ubc-K(BART19)/(E/S)/368 + "
 * 6) KAH107: (7) HPK-Kstop(KAH103)/(E/S)/546 + "
 * 7) KAH108: (8) HPK-K(KAH104)/(E/S)/540 + "

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations


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