User:Karmella Haynes/Notebook/Polycomb project/2010/08/10

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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08/10/10

 * &#x2713; Transfection: H3me Reporter lines (screen for YFP induction)
 * &#x2713; Microscopy: Fix cells (KAH130, 131 clones)
 * &#x2713; Western: protein preps for KAH130, 131 clones

Fugene transfection > 12-well plate Plate 1. KAH96 clones (6 total) Plate 2. KAH146 clones (6 total) Plate 3. KAH147 clones (6 total)

> Master mixes (20x mix, 3 mixes): 36.0 μL Fugene + 364.0 μL Opti-MEM --> R.T/ 5 min. > Add 20x vol DNA --> R.T./ 20 min. > Add 20 μL complexes to each well (1 ml med. each); Grow cells at 37&deg;C overnight


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