IGEM:Stanford/2010/Notebook/mRNA sRNA

General Ideas
One Idea to measure [B] / [A] (assume [A] ≠ 0)


 * input A induces expression of GFP mRNA


 * input B induces expression of GFP sRNA (sRNA that targets the GFP mRNA transcript)


 * so for a given [A],
 * if [B] = 0, we should see glowing bacteria
 * if 0 < [B] < [A], we should see some glow
 * if [B] > [A], we should see no glow

Another Idea to measure [B] / [A] (assume [A] ≠ 0)


 * input A induces expression of GFP mRNA and YFP sRNA


 * input B induces expression of YFP mRNA and GFP sRNA


 * so for a given [A],
 * if [B] = 0, we should see GREEN glowing bacteria
 * if 0 < [B] < [A], we should see some glow that is more GREEN than YELLOW.
 * if [B] > [A], we should see some glow that is more YELLOW than GREEN.