IGEM:NYMU/2007/Construct

Assembly Ordering and Progress system 1

system 2

Promoter assay construct: pOmpC+(RBS+EYFP)

OmpRBS assay construct: pLacIQ + OmpRBS + GFP OmpRBS assay construct: pLacIQ + OmpRBS

System 1: pOmpC + (TATA+ INSA) + (TATB+ INSB) + (RBS+ CinR) + (RBS+ HSL) + D-term Phase 1 of system 1: RBS + CinR

Phase 1 of system 1: RBS + HSL

Phase 1 of system 1: TATA + INSA = TATA_INSA

Phase 1 of system 1: TATB + INSB = TATB_INSB

Phase 2 of system 1: pOmpC + TATA_INSA

Phase 2 of system 1: (RBS+CinR) + (RBS+HSL) = CinR+HSL

Phase 3 of system 1: pOmpC + TATA_INSA + TATB_INSB

Phase 3 of system 1: CinR+HSL + D-term = CinR+HSL+D-term

Phase 4 of system 1: pOmpC + TATA_INSA + TATB_INSB + CinR+HSL+D-term

System 2: pCinRHSL+ OmpRBS + (TATD+IDE) + D-term Phase 1 of system 2: TATD + IDE = TATD_IDE

Phase 1 of system 2: pCinRHSL + OmpRBS

Phase 2 of system 2: pCinRHSL + OmpRBS + TATD_IDE

Phase 3 of system 2: pCinRHSL + OmpRBS + TATD_IDE + D-term

Basic Constructs pOmpC in pSB1A2

pCinRHSL in pSB1A2

RBS in pSB1A2

D-term in pSB1AK3