SynBERC:Drugs

Objective: Microbial synthesis of natural and unnatural organic compounds
This testbed is broken into two parts:


 * 1) Microbial synthesis of terpene indole alkaloids (TIAs), plant natural products
 * 2) de novo design of biosynthetic pathways

Parts

 * malic acid to malic semialdehyde converting enzyme


 * 7-deoxyloganin synthetic enzymes (from glucose + GPP)


 * vinblastine/vincristine transporter

Precursor Amplifiers

 * Over-expression of rate-limiting enzyme(s) for precursor formation


 * Simple expression device (promoter, RBS, ORF (protein coding), terminator)


 * Amp needs: tryptophan, geranyl diphoshpate (GPP), malic acid

Multi-cistronic/Multi-genic Biosynthetic Devices

 * Multiple ORFs with individual promoter/terminator pairs or as multi-cistronic operons

Metabolite Sensors
Needed to rapidly evaluate success in achieving synthesis goals.


 * secologanin
 * strictosidine
 * 3,4-dihydroxbutyric acid

Metabolite-controlled Biosynthetic Devices

 * Includes parts in multi-cistronic/multi-genic device


 * Metabolite-responsive expression (transcriptional control -- same switches can be used in sensors and control devices)
 * secolaganin
 * strictosidine
 * 3,4-dihydroxybutyric acid

Extracellular/Surface-associated Biosynthetic Device

 * Surface-anchoring protein part
 * promoter, RBS, ORF, terminator

Chassis

 * Precursor supply
 * tryptophan
 * geranyl diphosphate (GPP)
 * malic acid


 * Free glucose (PTS-independent) transport