BISC 219/F10:Gene Mapping

Background: Classical Forward Genetics and Gene Mapping Lab 2: Mutant Hunt Lab 3: Linkage Test Part 1 Lab 4: Linkage Test Part 2, Mapping and Complementation Lab 5: Mapping con't; complete complementation Lab 6: DNA sequencing analysis; Mapping con't Lab 7: Complete Mapping: Score!

Schedule of Experiments for Series 2
{| border="1" ! Lab # !! Dates !! Activity !! Outside Activity ! 2 ! 3 ! 4 Mapping: Pick Unc non-Dpy to separate plates;Complementation: cross unknown Dpy worms to wild type males
 * 9/14 - 9/20
 * Mutant Hunt
 * Confirm mutant phenotype and set up first cross
 * 9/21 - 9/27
 * Linkage crosses - heterozygous males with Unc hermaphrodites
 * Pick hermaphrodites for selfing
 * 9/28 - 10/4
 * Examine phenotypes to determine linkage
 * Mapping: Pick putative double mutants Complementation: cross heterozygous males with known Dpy mutants

! 5
 * 10/5 - 10/12
 * Mapping: Maintain double mutants (d u/d u): pick a few to individual plates Complementation: examine crosses for Dpy MALES
 * Nothing!

! 6 ! 7 SCORE! Find recombinant frequency
 * 10/13 - 10/19
 *  DNA Sequencing Analysis:Examine sequencing data to determine the lesion in your gene of interest & significance in gene product; Mapping:Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)
 * Bioinformatics:Use Wormbase and BLAST to learn more about the gene in other species; Mapping:Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)
 * 10/20 - 10/26
 *  Complete Mapping
 * Determine map distance between your Dpy and Unc marker