IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/21

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title

 * PCR followed by Dpn1 digestion of S03879 (Primers- VF2 and VR)
 * The following ligations were performed and they were grouped as D set of Ligations
 * R0040 + E0420 -> this is a part from the kit viz.I13600
 * R0040 + E0422 -> this is a part from the kit viz.I13602
 * R0040 + B0034 -> this is a part from the kit viz.J13002
 * J23119 + S03879 ---> J+S1 (Ligated using Standard assembly)
 * J23119 + S03879 ---> J+S2 (Ligated using Standard assembly)
 * Inoculated colonies from transformants of C group of ligations and the remaining Ligations of group B viz. K272502 and K272500.


 * }