IGEM:IMPERIAL/2006/LabCalendar/2006-8-13

To do today

 * Miniprep (37015RS & J37016)
 * Reculture and miniprep cre 1, 2, 4 & AiiA
 * Culture T9002 on request by Tom (2 mL M9, 2 mL LB)

Re-miniprep (J37015RS & J37016)

 * L2, 3, 4
 * M1, 2
 * V1, 2, 3
 * R1, 2
 * Miniprep product in fridge to be digested tomorrow (pending acquisition of PstI and more EcoR1)
 * 1 Day delay expected

Cultures

 * Culture in Liquid Media
 * Cre 1, 2, 4 (2 colonies each)
 * AiiA (4 colonies)
 * 12D->2H (4 colonies)

End of Sunday

 * Cultured Cre 1, 2, 4, AiiA and 12->2H in 100ml for maxiprep tomorrow
 * Cultured T9002 in 2ml of LB and M9 for testing tomorrow
 * Failed to miniprep Cre 1, 2, 4, AiiA and 12->2H

To do tomorrow

 * Complete digestion of J37015RS, J37016, Cre 1, 2, 4, AiiA, 12D->2H and run gel (from miniprep)
 * Maxiprep Cre 1, 2, 4, AiiA, 12D->2H in parallel with
 * T9002 testing