Image:PHAN+pKAN PDK PCR2.jpg

Gel of gradient PCR of the 741bp PDK intron out of pHANNIBAL and pKANNIBAL. Lanes are LtR lex. ordered by (temp, name) with temperatures 50:5:65ºC and names pHAN, pKAN. Improved contrast.

It appears that pHANNIBAL worked at 50, 60ºC but pKANNIBAL didn't.