User:Karmella Haynes/Notebook/BioBrick cloning/2009/11/20

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11/20/09

 * Minipreps: KAH86(2), 87(2), 88(2), 89(2), 90(2), 91(2), 92(2); KAH84(2, re-pick)
 * Digests: KAH77, 78, 79 (E/P/ScaI triple digests)
 * Assemblies: KAH011, 012; KAH93, 94, 95

Minipreps > Check with E/P digests > Include KAH77-79 E/P/ScaI digests (ScaI cuts V0120)

Assemblies
 * 1) KAH011: NLS-myc-stop/(X/P ds-oligo)/64 &#x2713; + (1) V0120/(X/P)/3200
 * 2) KAH012: Kstop/(X/P ds-oligo)/24 &#x2713; + "
 * 3) KAH93: (2) 2xHS4-Ubc-K-Gal4DB-PEST-2xmCh-NLS-stop (KAH77)/(E/S)/3480 + (5) 5xLuc target-polyA-2xHS4 (KAH67)/(E/X)/4553
 * 4) KAH94: (3) 2xHS4-HPK-K-Gal4DB-PEST-2xmCh-NLS-stop (KAH78)/(E/S)/3652 + "
 * 5) KAH95: (4) 2xHS4-CMV-K-Gal4DB-PEST-2xmCh-NLS-stop (KAH79)/(E/S)/3724 + "

> Digests (Fermentas FD) --> Triple digest KAH977-79 to cut V0120

> Measure conc.'s

> Ligations


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