IGEM:Cambridge/2008/Notebook/Voltage/2008/08/05

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Kdp?

 * There were transformants from yesterday's transformation




 * After Biobrick PCR the gel showed short fragments, suggesting self ligation of the vector.




 * Repeated digest and ligation protocol from the weekend, with EcoR1 and Pst1 instead of Xba1 and Spe.

Biobricks

 * Extracted two GFP-only sequences (J04630 and I714062) from the registry, for use as reporters for osmY.
 * PCR on these and the RBS B0030. Ran E-gel to check PCR.
 * Cut osmY plasmid with Spe, and RBS B0030 with Xba, and ligated the two.
 * Transformed cells with this construct. Then realised this was silly.


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