User:Fermenter User/Notebook/MACG F12

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs Purple: Induction hrs

MACG F12

Day -1 (Jan 13 Wed)
                1/11/2010 (14:53)  Prepare small culture for inoculum (20mL)

           1/13/2010 (14:00)  Pour media (850 ml) 1/13/2010 (14:00) pH calibration No Antifoam added 1/13/2010 (14:30) Autoclave bioreactor 4 empty bottles/fermenter Base, Carbon source+Inoculation, Metal ions, Feeding 1/13/2010 (15:40) Cooling & Start Temp Control 1/13/2010 (15:40) Start F1 software 1/13/2010 (15:41) Start F2 software 1/13/2010 (15:42) Start F3 software 1/13/2009 (16:00) Calibrate dO2 1/13/2009 (16:00) Set dO2

                5/12/2009 (22:23)  Prepare small culture for inoculum (200mL)

Day 0 (Jan 12 Tue)
           1/12/2010 (07:30)  Add carbon source using pumps 1/12/2010 (10:00) Connect Base (400 ml) Prime the pumps Set pH

               1/12/2010 (07:50)  Fermentation 00:00 Inoculation 50 ml (F1, F2, F3)                1/12/2010 (07:50) Fermentation 00:00 F1: OD (0.269) Contamination? (N) F2: OD (0.270) Contamination? (N) F3: OD (0.263) Contamination? (N)                1/12/2010 (10:00) Fermentation 02:10 F1: OD (0.365) Contamination? (N) F2: OD (0.384) Contamination? (N) F3: OD (0.360) Contamination? (N)                1/12/2010 (10:25) Fermentation 02:35 F1: OD (0.403) Contamination? (N) F2: OD (0.418) Contamination? (N) F3: OD (0.396) Contamination? (N)                1/12/2010  (10:40) Fermentation 02:50  Induction 00:00 F1: Induce with Galactose (55 mL) water (55 mL) Start feeding: (230uL/min) F2: Induce with Galactose (55 mL) supplement C1 (55 mL) Start feeding: (230uL/min) F3: Induce with Galactose (55 mL) supplement C3 (55 mL) Start feeding: <font style=background-color:yellow>(230uL/min)


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