User:Fermenter User/Notebook/BROM G1

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM G1 (F6)

Day 0 (Jan 26 Mon):
           1/26/2009 (10:45) Bring media to fermenter room           1/26/2009 (10:45) Assemble bioreactor 1/26/2009 (11:05) Add 10 ml of Antifoam. 1/26/2009 (12:25) Start Sterilization (Liq 45 min) 1/26/2009 (13:50) Cooling of bioreactor (Temp setup) 1 L/min air, 250 rpm 1/26/2009 (13:55) Start Fermentation Software for Fermenter #6 1/26/2009 (14:10) Connect Acid (500 ml) and Base (400 ml), prime pumps Start pH control 1/26/2009 (14:20) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 1/26/2009 (14:20) Set dO2 1/26/2009 (14:20) Completion of bioreactor set up: Ready for inoculation -               1/26/2009 (14:30) Inoculation: inoculation volume (300 ml) Fermentation 0:00            1/26/2009 (14:30) Sampling  Fermentation 0:00 Cell weight ( 2 mg), Activity (0 %, x100), Activity (0 %, x1000)

Day 1 (Jan 27 Tue):
Sung-Hye: CHBE Class Auditing 9:30 - 11:00

           1/27/2009 (8:30) Fermentation 18:00 Acid (450 ml) Base (215 ml) Increase rpm (from 400 to 700)             1/27/2008 (9:30) Sampling  Fermentation 19:00 Cell weight ( 131 mg), Activity ( 88%, x100), Activity (55 %, x1000)             1/27/2008 (15:00) Sampling  Fermentation 24:30 Cell weight ( 97 mg), Activity ( 89%, x100), Activity ( 67%, x1000) 1/27/2008 (16:00) Fermentation 25:30 Harvest


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