SBB09 Construct43

Construction of {} part

PCR Ons001F/Ons002R on TraT        (342 bp, BamHI/EcoRI) Digest pBca9495CA-Bca1144#5        (BamHI/EcoRI, 3039+910, L) Product is pBca9495-M10020          {} --- Ons001F Forward BglII for TraT ccataGAATTCatgAGATCTaaaccccgtaaacagcattcgcctg Ons002R Reverse BamH1 for TraT ctgatGGATCCggccaaatcaatcgcaaatattctcccccccgaaCtcaggc

JCA: I think you got the wrong template sequence somehow here. See revision below:

Construction of {} part

PCR Ons001F/Ons002R on TraT        (700 bp, BamHI/EcoRI) Digest pBca9495CA-Bca1144#5        (BamHI/EcoRI, 3039+910, L) Product is pBca9495CA-M10020          {} --- Ons001F	Forward oligo for TraT ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc Ons002R	Reverse oligo for TraT ctgatGGATCCgagaatatttgcgattgatttgg