Newman Lab

 Curriculum Vitae





 Name :     Newman Siu Kwan SZE

 Title :      Assistant Professor, School of Biological Sciences, Nanyang Technological University

 EMail :    sksze@ntu.edu.sg



 EDUCATION  :

 1990-1995               Ph.D., University of Hong Kong, Hong Kong

<p class=MsoNormal> 1987-1990               B.Sc., University of Hong Kong, Hong Kong

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<p class=MsoNormal><b> PROFESSIONAL APPOINTMENTS </b> :

<p class=MsoNormal> 2006.6 -  Now         Assistant Professor, Nanyang Technological University, Singapore

<p class=MsoNormal> 2002.7 – 2006.5      Group Leader, Genome Institute of Singapore, Singapore

<p class=MsoNormal> 2003.7 – 2006.5      Adjunct Assistant Professor, National University of Singapore, Singapore

<p class=MsoNormal> 2005.5 – 2006.5      Adjunct Assistant Professor, Nanyang Technology University, Singapore

<p class=MsoNormal> 2001.5 – 2002.6      Visiting Scientist, Cornell University, USA

<p class=MsoNormal> 1998.6 – 2001.5      Postdoctoral Fellow, University of Waterloo, Canada

<p class=MsoNormal> 1995.5 – 1998.5      Postdoctoral Fellow, University of Toronto, Canada

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<p class=MsoNormal style='margin-left:1.0in;text-indent:-1.0in'><b> <span style='font-size:10.0pt'>HONORS AND AWARDS </b> <span style='font-size:10.0pt'>:

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>1. Human Proteome Organization Annual Congress - First Prize Poster Award, 2004

<p class=MsoNormal style='text-indent:.25in'> Probing protein solvent accessible surface by laser induced oxidation and mass spectrometry

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>2. The Croucher Foundation Fellowships, 1995-97

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>3. The Croucher Foundation Studentships, 1990-93

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>4. Sir Edward Youde Memorial Scholarships, 1989-90

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>5. Du pont Science Scholarships, 1989-90

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>6. Dow Chemical Scholarships, 1989-90

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>7. G.T. Byrne Memorial Prize in Chemistry, 1989-90

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>8. Douglas Payne Prizes in Chemistry, 1989-90

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<p class=MsoNormal style='margin-left:1.0in;text-indent:-1.0in'><b> <span style='font-size:10.0pt'>INVITED SEMINARS: </b>

<p class=MsoNormal style='margin-left:.5in;text-indent:-.5in'><span style='font-size:10.0pt'>1. Workshop on Proteomics Data Analysis and Data Mining 2005

<p class=MsoNormal style='margin-left:.25in'> Algorithm for Quantitative Proteome Analysis

<p class=MsoNormal style='margin-left:.5in;text-indent:-.5in'><span style='font-size:10.0pt'>2. 5th HUGO Pacific Conference Nov 2004

<p class=MsoNormal style='margin-left:.5in;text-indent:-.5in'><span style='font-size:10.0pt'>        Fourier Transform Mass Spectrometry for Top Down Proteomics

<p class=MsoNormal style='margin-left:.5in;text-indent:-.5in'><span style='font-size:10.0pt'>3. The Mass Spectrometry Society of Japan - The 52nd Annual Conference 2004

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>        Application of Top Down Proteomics to Epigenetics and Gene Regulation

<p class=MsoNormal style='margin-left:.5in;text-indent:-.5in'><span style='font-size:10.0pt'>4. The Mass Spectrometry Society of Japan, Kanto-area Colloquium

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>        Probing Protein Tertiary Structure and Interaction by Mass Spectrometry

<p class=MsoNormal style='margin-left:.5in;text-indent:-.5in'><span style='font-size:10.0pt'>5. Pharmacoproteomics 2003, Tokyo, Japan

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>        Top Down Approach for Proteomics Analysis by Fourier Transform Mass Spectrometry and Electron Capture Dissociation

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>6. A Workshop on Introduction to BioMEMS and BioMedical Applications 2003, Institute of Microelectronics, Singapore

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>        Coupling Capillary Isoelectric Focusing in Open Microchannel to Mass Spectrometry for Protein Characterization

<p class=MsoNormal style='margin-left:.5in;text-indent:-.5in'><span style='font-size:10.0pt'>7. Proteomics in Asia and Oceania 2002, Osaka, Japan

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>        Fourier Transform Mass Spectrometry and Electron Capture Dissociation for Protein Characterization

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<p class=MsoNormal style='text-autospace:none'><b> <span style='font-size: 10.0pt'>SCIENTIFIC SERVICES: </b>

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in;text-autospace: none'> 1. Editorial Board, Journal of Mass Spectrometry Society of Japan

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in;text-autospace: none'> 2. Proposal Reviewer, Hong Kong Research Grant Council

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in;text-autospace: none'> 3. Proposal Reviewer, BMRC, Singapore

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 RESEARCH INTERESTS: 

<p class=MsoNormal style='text-align:justify'> Our research is devoted to develop and apply advanced proteomic technologies to decipher human diseases through a rational search for novel disease targets and medicines. Tandem affinity purification tags have been constructed and utilized to study proteins that are important to transcriptional and translational regulatory networks, DNA repair, and apoptotic pathway. To this end, we have studied Ku70 protein complex and identified several new partners in the nucleus that function in DNA repair and transcriptional regulation. More importantly, we have found that Ku70 played a key role in signal transduction and governed the master switch in apoptotic pathway in the cytoplasm. Currently, we focus our efforts to understand the role of Ku70 in cancer development and other human diseases. Another theme of our research is directed to develop high sensitive structural based proteomic methodologies to elucidate the molecular machine operating inside the cell. We have developed a novel laser induced photochemical protein surface oxidation method to determine protein (or protein complex) solvent accessible surface. We are applying this technology to map sites of protein-protein, protein-DNA/RNA, and protein-drug interactions, and to study their binding kinetics.



<p class=MsoNormal> In addition, we are developing cutting-edge top-down proteomic technologies based on LC-MS/MS and LTQ Orbitrap Mass Spectrometry. Protein functions are highly regulated by their post translational modifications such as phosphorylation, acetylation and methylation. These modifications are central to cellular signaling pathways and gene regulatory networks, and therefore important for cancer therapy and diagnostics. However, it is difficult to study these modifications using traditional biochemical methods. The top-down proteomic strategy will enable us to characterize and profile global protein post-translational modifications.

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<p class=MsoNormal style='margin-left:1.0in;text-indent:-1.0in'><b> <span style='font-size:10.0pt'>SELECTED PUBLICATIONS: </b>

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>1. Chen L, Sze SK and Yang H, <i>Automated Intensity Descent Method (AID-HRMS) for Efficient Interpretation of Complex High Resolution Mass Spectra of Proteins, </i>Analytical Chemistry, 2006. (In Press).

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>2. Wong JJY, Pung YF, Sze NSK and Chin KC, <i>HERC5 is a novel interferon-induced HECT-type E3 protein ligase that mediates Type I interferon-induced ISGylation of protein targets,</i> Proceedings of the National Academy of Sciences of the USA, 2006. (Accepted).

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>3. Guo X, Chan-Park M, Yoon SF, Chun J, Hua L, and Sze NSK, <i>UV Embossed Polymeric Chip for Protein Separation and Identification based on Capillary Isoelectric Focusing and MALDI-TOF-MS</i>, Analytical Chemistry,  78, 3249-3256, 2006.

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>4. Hua L, Low TY, and Sze NSK, <i>Microwave-assisted Specific Chemical Digestion for Rapid Protein Identification, </i>Proteomics, 6, 586-591, 2006.

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>5. Aye TT, Low TY, and Sze NSK, <i>Nanosecond Laser Induced Photochemical Oxidation method for Protein Surface Mapping with Mass Spectrometry,</i> Analytical Chemistry, 77(18), 5814-5822, 2005.

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>6. Mok MLS, Hua Lin, Phua JBC, Wee MKT, and Sze NSK, <i>Capillary Isoelectric Focusing in Pseudo-closed Channel Coupled to Matrix Assisted Laser Desorption/Ionization Mass Spectrometry for Protein Analysis</i>, Analyst, 129, 109-110, 2004.

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>7. Sze NSK, Ge Y, Oh H, and McLafferty FW, Plasma Electron Capture Dissociation for Large Protein, Analytical Chemistry, 75, 1599-1603, 2003.

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>8. Ge Y, ElNaggar M, Sze NSK, Hanbin Oh, Begley TP, and McLafferty FW, <i>Top Down Proteomic Analysis of Secreted Proteins from Mycobacterium Tuberculosis by Electron Capture Dissociation Mass Spectrometry</i>, Journal of the American Society of Mass Spectrometry, <b>14(3), </b>253-261, 2003.

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>9. Ge Y, Lwahor BG, ElNaggar M, Sze NSK, Begley TP, and McLafferty FW, <i>Protein Oxidation in Viral Prolyl-4-hydroxylase Characterized by Top Down Tandem Mass Spectrometry</i>, Protein Science, 12, 2320-2326, 2003.

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>10. Oh H, Breuker K, Sze SK, Ge Y, Carpenter BK, and McLafferty FW,  <i>Secondary and tertiary structures of gaseous protein ions characterized by electron capture dissociation mass spectrometry and photofragment spectroscopy.</i> Proceedings Of The National Academy Of Sciences Of The United States Of America, <b>99, </b>15863-8, 2002.

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>11. Sze NSK, Ge Y, Oh H, and McLafferty FW, <i>Top Down Mass Spectrometry of a 29 kDa Protein for Characterization of Any Posttranslational Modification to Within One Residue</i>, Proceedings of the National Academy of Sciences of the USA. 99, 1774-1779, 2002.

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<p class=MsoNormal style='margin-left:1.0in;text-indent:-1.0in'><b> <span style='font-size:10.0pt'>PATENTS: </b>

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>1. Capillary Isoelectic Focusing – MALDI Device for Protein Analysis

<p class=MsoNormal style='margin-left:.25in;text-indent:-.25in'><span style='font-size:10.0pt'>2. Polymer Composite for MALDI Analysis of Biomolecules.

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<p class=MsoNormal> Lab Members: 

<p class=MsoNormal> Ran Dong Yuan

<p class=MsoNormal> Email: rdyuan@ntu.edu.sg

<p class=MsoNormal> Tel: (65) 6316-2852

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<p class=MsoNormal> Meng Wei

<p class=MsoNormal> Email: g0402992@nus.edu.sg

<p class=MsoNormal> Tel: (65) 6316-2852

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<p class=MsoNormal> NARDEV S/O RAMANATHAN

<p class=MsoNormal> Email: nardev@pmail.ntu.edu.sg

<p class=MsoNormal> Tel: (65) 6316-2852

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<p class=MsoNormal> Zhao Chen

<p class=MsoNormal> Email: 149914862@ntu.edu.sg

<p class=MsoNormal> Tel: (65) 6316-2852

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<p class=MsoNormal> Vacancies: 

<p class=MsoNormal> We are looking for Postdoctoral Fellows, Research Assistants and Graduate Students to join our multi-disciplinary team in the following areas:

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<p class=MsoNormal style='margin-left:.5in;text-indent:-.25in'><span style='font-size:10.0pt'>1)       proteomics technology development: background in physics, chemistry, biology or engineering

<p class=MsoNormal style='margin-left:.5in;text-indent:-.25in'><span style='font-size:10.0pt'>2)       proteomics bioinformatics/mass spectrometry data analysis: Mathematics, Computer Sciences

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