User:Tara K. Luckau/Notebook/Team ConGen/2010/11/29

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 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
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 * style="background-color: #9DB68C" align="center"|  |Main project page


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Make Low TE

 * 1) 500 mL of Low TE, pH 8.0:
 * 2) * 10 mL of 0.5M Tris-Cl, pH 8.0
 * 3) * 0.1 mL of 0.5M EDTA, pH 8.0
 * 4) * 489.9 mL of ddH2O
 * 5) pH = 7.97
 * 6) Autoclave
 * 7) * middle autoclave
 * 8) * #3 (121°C, 45 min)
 * 9) pH = 7.91

Make KCl

 * 1) 125mL of 3M KCl
 * 2) * 27.96g KCl
 * 3) * add NanoPure H2O to bring to 125mL


 * Used:
 * Molecular Sigma Biology
 * Potassium Chloride, KCl, FW=74.55g/mol
 * Catalog # P-9541
 * Lot # 34H030215


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