Tiffany Guo

About me
Name: Tiffany Guo Course/Minor: 20/5 Year of Graduation: 2009 Email: tiffguo Current Classes: 5.03, 6.00, 20.109, 21F.108

Day 4: Genome Refactoring
I refactored the phate genome to remove overlaps in the genome and to add in restriction enzyme sites between each part. I edited codons to retain amino acid sequence while weakening the rbs or promoter. Considerations: 1) eliminate rbs and promoters within genes: took into consideration codon changes that would not change amino acid sequences but would decrease the strength of the rbs or promoter. 2) add restriction sites between each part: tried to keep sticky ends as consistent as possible (i.e. after every promoter, the restriction site sticky end is 3'ctag), however, there aren't enough zero cutting isoschizomers

On the parts registry, my part is BBa_M31975.

New Parts: a) restriction enzyme sites b) Trancription terminator

c) coding sequences: