IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/10/Zhangs

Electrophoresis 12 samples of PCR identification products. Sample loading order: T7p+5N+CI+T①~⑤, T7P+2G+CI+T①~⑤, T7P+2I+CI+TL①~②, Marker The T7P+5N+CI+T①-⑤ & T7P+2G+CI+T①-⑤ & T7P+2I+CI+T①,are target spawns (PCR identified).

Electrophoresis another 12 samples of PCR identification products. Sample loading order: T7p+2I+CI+T③~⑤, T7P+2M+CI+T①~⑤, T7P+11N+CI+T①~④, Marker The T7P+2I+CI+T③-⑤ & T7P+2M+CI+T①-⑤ & T7P+11N+CI+T②-④ are target spawns (PCR identified). Electrophoresis another 12 samples of PCR identification products. Sample loading order: T7p+11N+CI+T⑤, T7P+1J+CI+T①~⑤, T7P+1H+CI+T①~⑤, LinMIin, Marker The T7P+1J+CI+T①-⑤ & T7P+1H+CI+T④ are target spawns (PCR identified).

Transfer the colonies into tubes with 5mL LB: T7P+1J+CI+T①; T7P+5N+CI+T①; T7P+2G+CI+T①; T7P+2I+CI+T①; T7P+2M+CI+T①; T7P+11N+CI+T②; T7P+1H+CI+T④.