DeLong:Lab Protocols:Julie's protocols

Julie's Protocols
A warning: these all work for me, but molecular biology is a mysterious thing, so they may not work for you! Please email me at jmaresca (at) mit (dot) edu if you have a question or a problem. Thanks.

[[Media:Competent cells.pdf|Preparation of electrocompetent cells.]] This works for E. coli, and I have not tested it on any other bacterial species.

[[Media:PlasmidMini.pdf|Plasmid Minipreps.]] This is a standard miniprep protocol.

[[Media:PCR_jm.pdf|Standard PCR reactions ]] (for normal amplification and for sequencing).

Use of the [[Media:Delong_Sonicator.pdf|Sonicator]] for small bacterial samples.

[[Media:HPLC_Tokyo.pdf|Reverse-phase HPLC analysis of carotenoids and (bacterio)chlorophylls.]]

[[Media:HPLC-Ecoli-rev.pdf|Reverse-phase HPLC analysis of carotenoids produced in E. coli.]]