User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/09

{| width="800"
 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Individual Rotifer Extraction fresh from Moss sample
1) Soak fresh Moss sample in petri dish with autoclaved/filtered spring water - enough to cover sample. 2) Wait 1 hour and/or move sample around in water. 3) Search for rotifers. Maybe helpful to remove moss to search. 4) Onto a petri-dish, place 1 rotifer in 50ul of autoclaved/filtered spring water. Rinse 3 to 5 times with fresh autoclaved/filtered spring water, taking care not to suck up rotifer. Attempt to remove all debris leaving only single rotifer. 5) Pick rotifer with ~50ul of clean autoclaved/filtered spring water and place in extraction tube. 6) Proceed with Instagene extraction.

Loaded 2.5ul PCR product



LCO/HCO
Loaded 2.5ul PCR product