IGEM:Harvard/2006/vlau/Notebook/2006-8-2

Folding Rxn
1. Goal: 6 samples of 100 6 hb

2. Reaction mixture: 50 p7308 scaffold 40 oligos 10 10x folding buffer (500mM HEPES ph 7.5, 500mM NaCl, 100mM MgCl2) neg. controls: 1 replacing oligos w/ water, 1 replacing scaffold w/ water

3. Image (2% agarose gel + 11 mM )

PEG Precipitation
1. Goal: repeat titration of PEG precipitation conditions for 2 samples of 6hb

2. Protocol

- incubated on ice for 15 min. - spun @ 16k rcf for 10 min.     - pipetted supernatant into separate tube - pellet resuspended in 1x folding buffer volume equal to supernatant - ran equivalent volumes of pellet and supernatant on 2% agarose gel (+11 mM )

3. Gel Analysis