Prince:BYU Lipidomics Team

Basic Protocol

 * Get sample from almost any source
 * Extraction (both methods use Folch reagent [2:1 chloroform:methanol])
 * use Kaluzne method
 * trying to implement Bligh & Dyer (gold standard)
 * MTBE Extraction
 * Mass Spectrometry
 * ESI-Agilent TOF
 * Tandem MS-Q-Star (8-10am)
 * Analysis
 * Matlab (particular toolbox?) PCA analysis

Main analytes are phosphatidylcholine and phosphatidylethanolamine. Analytes in (-) mode would be phosphatidylserines and phosphatidylethanolamines (which are expected to have stronger signal in - mode). Typical number of analytes?

FAME-GC associated with Craig Thulin and UVU.

Lipidomics
Lipidomics and lipid profiling in metabolomics |14|sl_10

Lipidomics: coming to grips with lipid diversity, Nature Reviews Molecular Cell Biology, 11, 593–598 (1 August 2010)

Mass Spectrometry
Practical essentials of ESI.*

Solid tutorial review on mass spec "-omics."*

General approaches to lipidomics. 

Recent advances (2011) in MS lipidomics. 

Basics of ESI and ESI intrasource separation shotgun lipidomics.**

Factors influencing ESI intrasource separation. 


 * Sample preparation for intrasource separation.**

Multiple precursor ion and neutral loss scanning with data-dependent acquisition. 

Top-down lipidomic strategy.**

Top-down lipidomic analysis of blood plasma.*

Yeast quantitative shotgun lipidomics. 

Bioinformatics
Informatics and computational strategies for the study of lipids. 

Computational lipidomics. 

Chemometrics for Metabonomics. 

LipidXplorer: open-source lipid identification and quantification software. 

PCA package. 

K-OPLS package. 

S-plot. 

Special Journal Issues on Lipidomics
Journal of Chromatography B, Volume 877, Issue 26,

Prostaglandins and Other Lipid Mediators, Volume 77

European Journal of Lipid Science and Technology, Volume 111, Issue 1

Frontiers in Bioscience, Volume 12, January 2007

Methods in Enzymology, Volume 432, 2007

Signalling Lipids and Lipidomics
Sphingolipid signalling. 

Lipid mediators in health and disease. 

Lipid mediators for inflammation resolution. 

Eicosanoid lipidomics. 

Bioactive lipidomics. 

Lipid mediator informatics-lipidomics. 

Mediator lipidomics. 

Current

 * Mary Blackburn
 * Mackay Merrill
 * Dean Flanders
 * Landon Weist
 * Rob Roden
 * Greg Jackson
 * Daniel Edmondson
 * Matthew Linford
 * Craig Thulin
 * Steven Wood
 * John Prince

Former or Associated

 * Jennifer MacDonald
 * Jonathan Lee
 * Vinod Chaudhary
 * Steven Herron

Relevant Publications
// This has a great protocol for negative ion mode extraction and analysis on the Orbitrap.
 * 1) P1 pmid=19935646