User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/09

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12/09/09

 * &#x2713; Cultures: KAH109-116 (2 each)
 * &#x2713; Assemblies: KAH117-125 (miR sensor Repressor/Target pairs)
 * &#x2713; Sequencing order results

Assemblies
 * 1) KAH117: (1) KAH99/(E/S)/2913 + (4) KAH93/(E/X)/8039
 * 2) KAH118: (2) KAH100/(E/S)/3085 + "
 * 3) KAH119: (3) KAH101/(E/S)/3157 + "
 * 4) KAH120: KAH99/(E/S)/2913 + (5) KAH94/(E/X)/8211
 * 5) KAH121: KAH100/(E/S)/3085 + "
 * 6) KAH122: KAH101/(E/S)/3157 + "
 * 7) KAH123: KAH99/(E/S)/2913 + (6) KAH95/(E/X)/8283
 * 8) KAH124: KAH100/(E/S)/3085 + "
 * 9) KAH125: KAH101/(E/S)/3157 + "

> Digests (Fermentas FD) --> KAH99, 100, 101: E/S/ApaLI

> Measure conc.'s

> Ligations

Sequencing order results
 * 1) KAH93/MV2 - DDB122B (fwd): good
 * 2) KAH93/MV2 - DDB123B (rev): good
 * 3) KAH94/MV2 - DDB122B (fwd): good
 * 4) KAH94/MV2 - DDB123B (rev): okay (1 HS4 and one partial, then non-specific sequence)
 * 5) KAH95/MV2 - DDB122B (fwd): good
 * 6) KAH95/MV2 - DDB123B (rev): good
 * 7) KAH99/MV2 - DDB122B (fwd): good
 * 8) KAH99/MV2 - DDB123B (rev): good, polyA different from seq on file, but matches other clones
 * 9) KAH100/MV2 - DDB123B (fwd): good
 * 10) KAH100/MV2 - DDB123B (rev): good, polyA different from seq on file, but matches other clones
 * 11) KAH101/MV2 - DDB122B (fwd): good
 * 12) KAH101/MV2 - DDB123B (rev): good, polyA different from seq on file, but matches other clones
 * 13) KAH105/MV2 - DDB122B (fwd): HPK-K-Gal4DB (KAH108)? (should be Ubc-Kstop-Gal4DB)
 * 14) KAH105/MV2 - DDB123B (rev): good
 * 15) KAH106/MV2 - DDB122B (fwd): Ubc-Kstop-Gal4DB (KAH105)? (should be Ubc-K-Gal4DB); Ubc is missing a C at 21 bp
 * 16) KAH106/MV2 - DDB123B (rev): good
 * 17) KAH107/MV2 - DDB122B (fwd): Ubc-K-Gal4DB (KAH106)? (should be HPK-Kstop-Gal4DB); Ubc is missing a C at 21 bp
 * 18) KAH107/MV2 - DDB123B (rev): good
 * 19) KAH108/MV2 - DDB122B (fwd): HPK-Kstop-Gal4DB (KAH107)? (should be HPK-K-Gal4DB)
 * 20) KAH108/MV2 - DDB123B (rev):


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