SBB09 PCR Cloning

Dilute oligonucleotides to be 10 uM


 * Add 1 ul of oligo dilution to 9 ul of ddH20
 * Mix and spin

Cloning PCR, (products are 937bp & 1600bp, )
 * 24 uL water
 * 3.3 uL Expand 10x Buffer 2
 * 3.3 uL 10x dNTPs (2 mM in each; 0.2 mM final conc)
 * 1 uL Oligo Odvs007F (10uM)
 * 1 uL Oligo Odvs008rR (10uM)
 * 0.5 uL Expand Polymerase 1
 * 0.5 uL Template DNA E.coli 0157:H7
 * Run this PCR on program 55