Choano Food Preperation

Procedure

 * Add all 100 or 150uL of an MC1061 competent cell tube to 400mL LB
 * Grow up overnight on 37*C shaker
 * split into two centrifuge bottles and centrifuged at 4200rpm for 14 minutes
 * pour off supernatant
 * wash each pellet with 10mL artificial sea water (ASW)
 * pour off ASW
 * add 10mL ASW to each bottle and resuspend pellets
 * centrifuge at 4200rpm for 14 minutes
 * pour off supernatant
 * re-suspend pellets
 * combine the contents of the two bottles
 * create 2uL aliquots