Axel:Schwekendiek Tutorials

  Home  Teaching Protocols Tutorials 

The material on this page has been "stolen" from Prof. John C. Anderson at UC Berkeley. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. Updated tutorials will be marked with this icon. Until this happens, I hope John will keep his valuable pages online and the links working. If the links become dead, please let me know and will hurry replacing the information. Pages marked with are a "must read"!

The Basics
'''Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics. ''The material in this section needs to be understood by everyone before starting lab work. It is essential. But I assume this knowledge is present from other courses and I won't go over it (except upon request).'

Bare bones biochemistry Basic Molecular Biology Practical DNA What is PCR?

Introduction to Biobricks and Basic Cloning
'''This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it. '''

Installing ApE - this program is not required for the UNI course! Overview of Cloning Introduction to Biobricks Working with bacterial stocks Introduction to Refactoring

Technical Design
Basic Oligo Design Special Case: Single Internal Restriction Sites Construction of short parts Design of composite parts Keeping track of construction files Sequencing Analysis

Advanced Technical Design
Working with Translational Fusions Circularly Permuted Proteins The "what did they make" game

Implementation
Basic Gene Recombination

Introduction to Pathway Engineering
coming sooner coming soon

Things you should know
Common Pitfalls and Tips Origins of replication The Critical Path