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Justin Wang

SssI methyltransferas part jtk2259



This part exists but requires an EcoRI/BamHI transfer
The SssI methyltransferase methylates DNA at sites analogous to CpG methylation in eukaryotes. It comes from a bacterium, and it is sold in recombinant form by NEB. Their website (http://www.neb.com/nebecomm/products/productM0226.asp) has more information and links about the protein. Unfortunately, it's a little toxic, and we want to see if we can figure out why and if we can optimize its expression through use of a stress promoter. This part already exists, but it is with the wrong vector. You'll need to do an Eco/Bam transfer to create the right plasmid. Your starting material is pBca9145-jtk2259, and we need to subclone it into pBca9523-Bca1144#5. P_fimF/I part sbb1110

P_cysI part sbb1129