Van Oudenaarden Lab:R08B4.2

Progress:

Probes designed: (03-01-2010 - Alexander van Oudenaarden)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

>R08B4.2 (alr-1 spliced coding region + 5'-UTR)

ttttcgcttccttctttcaactttggtaaatcgtttcaacttggattcacattgtgt

atgcccgagttgaagaaagaagactcttccaaagacgagaaagatctagacatgaactgcccgccacttcacaggcccaa tggagctgatttgaatcagtattcaaaaagtcttatggaacagttacaggcacaattgtttgcaaatccagcgttacagt tcccatcatttccaccagctttttctattgcggcgctgactaataatcagcatgagctgaaagaagacgatggaaagaaa acgcccactggagataatattttagaagctgcctcagtacttgacaatagagaaaacggatccccttcagatggcacaaa cagtccggatgataatggaaagaggaaacaaagaagataccgtacaacattcagtgcatttcagttggacgagcttgaga aggtgtttgccaggactcactatccagatgtgttcactcgggaagaattggcaacacgagtccagctcacagaggcccga gttcaagtttggtttcaaaacagacgagccaagtatcgcaaacaagaacgcagcagtactcatcacccttatcaagctcc aatgagtattccaaattcaaatggagataatccgtaccagatgatgctcagccaagaagctatttttgcagctatcaatc agcaagccgcggctcatcttttaaatgaacaagtacggattgcaacatctgatcgtcggtctcagtccccgtctgtacca gtgaccacatcttctccaatcttgccaacttctgtgacacctacgttccaaaacgcagatgcattaaatatgttgttcgg tggaattactcctgttacgcagcagttgctttatgttcaacaattcagcagagctatggatgctttccgcactcagcttc tgggatcagtcccagcaggagctacggctgaagtcaccgatgttgtagcattaaaaactgaagactcgaaatcgaatagt agagcgggaagctcatcacccactccaactgaaagttccggagctgctgcaacatccacgagtcctacgaattttgctga catgaatagtctgatatccgatgtgaagccaaaagaagaaagttcatga

Name of probe set: alr-1

Probes designed on Mon, 01 Mar 10 08:46:44 -0700

48 probes designed for target of length 1146

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

gttgaaagaaggaagcgaaa, alr-1_1, 1, 2, 40

ccaagttgaaacgatttacc, alr-1_2, 2, 25, 40

aactcgggcatacacaatgt, alr-1_3, 3, 49, 45

cgtctttggaagagtcttct, alr-1_4, 4, 75, 45

ggcagttcatgtctagatct, alr-1_5, 5, 99, 45

ttcaaatcagctccattggg, alr-1_6, 6, 133, 45

ctgttccataagactttttg, alr-1_7, 7, 161, 35

ttgcaaacaattgtgcctgt, alr-1_8, 8, 183, 40

atgatgggaactgtaacgct, alr-1_9, 9, 207, 45

gcaatagaaaaagctggtgg, alr-1_10, 10, 229, 45

atgctgattattagtcagcg, alr-1_11, 11, 251, 40

ttccatcgtcttctttcagc, alr-1_12, 12, 273, 45

ttatctccagtgggcgtttt, alr-1_13, 13, 295, 45

gtactgaggcagcttctaaa, alr-1_14, 14, 318, 45

gatccgttttctctattgtc, alr-1_15, 15, 340, 40

actgtttgtgccatctgaag, alr-1_16, 16, 362, 45

tcctctttccattatcatcc, alr-1_17, 17, 384, 40

gttgtacggtatcttctttg, alr-1_18, 18, 406, 40

gtccaactgaaatgcactga, alr-1_19, 19, 428, 45

tggcaaacaccttctcaagc, alr-1_20, 20, 450, 50

gaacacatctggatagtgag, alr-1_21, 21, 473, 45

actcgtgttgccaattcttc, alr-1_22, 22, 499, 45

ttgaactcgggcctctgtga, alr-1_23, 23, 524, 55

gctcgtctgttttgaaacca, alr-1_24, 24, 547, 45

gcgttcttgtttgcgatact, alr-1_25, 25, 569, 45

gataagggtgatgagtactg, alr-1_26, 26, 591, 45

tttggaatactcattggagc, alr-1_27, 27, 613, 40

ctggtacggattatctccat, alr-1_28, 28, 638, 45

atagcttcttggctgagcat, alr-1_29, 29, 661, 45

ttgctgattgatagctgcaa, alr-1_30, 30, 683, 40

catttaaaagatgagccgcg, alr-1_31, 31, 705, 45

gatgttgcaatccgtacttg, alr-1_32, 32, 727, 45

aagatgtggtcactggtaca, alr-1_33, 33, 771, 45

acagaagttggcaagattgg, alr-1_34, 34, 793, 45

atctgcgttttggaacgtag, alr-1_35, 35, 818, 45

gtaattccaccgaacaacat, alr-1_36, 36, 847, 40

aaagcaactgctgcgtaaca, alr-1_37, 37, 870, 45

gctctgctgaattgttgaac, alr-1_38, 38, 892, 45

aagctgagtgcggaaagcat, alr-1_39, 39, 917, 50

tagctcctgctgggactgat, alr-1_40, 40, 942, 55

acaacatcggtgacttcagc, alr-1_41, 41, 964, 50

cgagtcttcagtttttaatg, alr-1_42, 42, 986, 35

ttcccgctctactattcgat, alr-1_43, 43, 1008, 45

tcagttggagtgggtgatga, alr-1_44, 44, 1030, 50

atgttgcagcagctccggaa, alr-1_45, 45, 1053, 55

gcaaaattcgtaggactcgt, alr-1_46, 46, 1075, 45

cggatatcagactattcatg, alr-1_47, 47, 1098, 40

ctttcttcttttggcttcac, alr-1_48, 48, 1120, 40