IGEM:IMPERIAL/2007/Calendar/2007-8-28

We are in Week 8  of the iGEM project

Project Calendar
name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7

Phase 1
Inital testing of the biobricks
 * pTet works
 * doesn't work below 10&deg;C or above 45&deg;C
 * fluorescence peak at 25&deg;C - needed a monotonic relation between temperature and GFP expression
 * pT7 - doesn't work - being re-cloned
 * pLux - works at quite a high [AHL]
 * not very good fluorescence for a realistic [AHL]
 * no GFP for 1nM of AHL
 * possible to change the threshold of the construct?

Cell extract testing

 * Home-made cell extract with home-made reaction buffer - doesn't work
 * Flooded the extract with DNA - still dosn't work
 * Need more comercial cell extract - budget limit almost reached
 * Need to plan experiments more efficiently

Modelling

 * Finished models for construct 1 and 2
 * Currently documenting on the wiki
 * Preparing for presentation

Vessicles

 * Prepared presentation for Wednesday
 * Done calculations for changes in protocols - to make the development of vessicles better
 * Test again tomorrow
 * Might have found a non-toxic method to create pores on vessicles - need further investigation
 * Will affect stability and life-span

Cloning of biobricks

 * All working biobricks cloned
 * Making substitutes for the ones not woorking
 * New parts to be added to the registry
 * Waiting for dsRed to arrive
 * No further ligations
 * Purified LuxR - has been ordered (USA)
 * Will pursue both construct 1 and 2 for ID