IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/06/27

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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June 27, 2009
TL1 NY4 AK1 1 μL 10 NEB buffer 1μL 10X BSA 5μL ddH2O .5+.5μL restriction enzymes. Heat in 37C water bath for one hour.
 * Miniprep of single colonies picked Friday evening picked from Nora's plate
 * Tony Annie and Nora all picked their own colonies with 200mL pipette tip, grew overnight in 3mL LB+Amp media
 * Restriction double digest with BamHI/BglII
 * -Enzyme controls for each sample, 1 -DNA control for prep

1kb Ladder, -DNA, TL, TL -Enz, NY, NY -Enz
 * Ran 1.2% Agarose gel with only Tony and Nora's cultures (All three grew, but will not need all DNA)


 * Bands look good. Will use Tony's DNA ("TL1" for future PCRs).

From 7/1 NY4: 426, TL1: 336 μg/mL
 * Reminder! Need to take concentrations.


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