Sauer:Purification of His-tagged proteins/Native prep

=Introduction= This is a general protocol for purification of His6-tagged proteins under native conditions. Note that you may need to modify this protocol to fit your specific needs. This protocol is a modified version of that provided with Ni2+-NTA resin from Qiagen. =Buffers=

Lysis buffer
50 mM NaH2PO4

500 mM NaCl

10 mM imidazole

Adjust to pH 8

Wash buffer
50 mM NaH2PO4

500 mM NaCl

20 mM imidazole

Adjust to pH 8

Elution buffer
50 mM NaH2PO4

500 mM NaCl

250 mM imidazole

Adjust to pH 8