IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/21/Shan Shen

11:00 PCR the clones of high-copy bi-stable system to test if they are correct.

13:30 Electrophoresis to test the PCR products. The order and the amount of the samples: marker 5μL, bi-stable 1, bi-stable 2, bi-stable 3. Results: All of the three clones are incorrect according to the results of PCR.

14:21 Miniprep bi-stable 1,2,3,SupD1 and 1-9H. Concentrations:

16:50 Digest the bi-stable system to test whether the ligations are correct

17:15 Start to digest.

20:00 Electrophoresis to test the digestion products. The order and the amount of the samples: marker 5μL, bi-stable plasmids1, bi-stable 1 digestion products, bi-stable 2 plasmids, bi-stable 2 digestion products, bi-stable plasmids 3, bi-stable 3 digestion products. Results: