Jeff Tabor/MEGAWHOP

Reaction setup

 * 120ng template plasmid
 * 500ng Megaprimer
 * Pfu Ultra II

Cycling conditions
95C, 2' 95C, 20" 55C, 20" 68C, 6-12' 25x

Transformation

 * DpnI, 1-2h
 * Qiaclean the reaction
 * Transform 0.5-2uL clean DNA into 50uL chemically competent E.coli
 * Plate all