IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/16

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6/16/10
12:30 PM

Promoter BBa_J23119: had growth on both plates for new and used cuvettes. Took 1 colony from new cuvette plate (50 ul plated) and inoculated 5 mL LB broth.

Gas Vesicles BBa_I750016:

made glycerol stock: .9 mL 10% glycerol and .9 mL culture in tube in -80°C freezer.

Miniprepped (promega kit) remaining sample and nanodropped (432.57 ng/ul)

PCR

used 100ng DNA as template

19.5 ul mastermix and .5 ul template DNA to each reaction (except negative control which gets .5 ul H2O instead).

Samples: BBa_I750016, negative control.

Program: BBa6Kb

Size: ~6Kb

Primers: Biobrick sequencing forward and reverse.


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