User:Karmella Haynes/Notebook/Polycomb project/2010/08/03

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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08/03/10

 * &#x2713; Transfection: Test H3me Reporter clones
 * &#x2713; KAH126-3: expand thawed cells
 * &#x2713; Senescence assay: 6-well plates, -/+ dox, for KAH126-1, 126-3, 132-8, 154-2, FTRx (rotenone, dox, DMSO, u.t.)
 * &#x2713; Cultures: split confluent plates

Fugene transfection > 12-well plate Plate 1. KAH96 clones (6 total) Plate 2. KAH146 clones (6 total) Plate 3. KAH147 clones (6 total)

> Master mixes (20x mix, 2 mixes): 36.0 μL Fugene + 364.0 μL Opti-MEM --> R.T/ 5 min. > Add 20x vol DNA --> R.T./ 20 min. > Add 20 μL complexes to each well (1 ml med. each); Grow cells at 37&deg;C overnight

08/04/10 Results: No YFP

08/05/10 Results: Screened for YFP again today and saw lots of YFP+ cells in KAH146-1; re-plate KAH96 & 147 for another transfection


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