Griffitts:Protocols

Recipes

 * Common buffers
 * Common media
 * Stock solutions

DNA

 * Phylogenetics

PCR

 * PCR primers
 * Standard PCR
 * Overlap-extension PCR
 * Arbitrary PCR
 * PCR clean-up (Qiagen)
 * Sequencing prep

DNA prep

 * Qiagen Miniprep
 * Low-copy plasmid prep
 * Nucleobond Midiprep
 * Genomic DNA prep (Qiagen)
 * Gel electrophoresis
 * Modified Eckhardt gel
 * Restriction digest
 * In-gel ligation

RNA

 * Trizol RNA purification (bacterial)
 * DNAse treatment
 * Reverse transcription
 * 5' RACE

Protein

 * Gel recipes
 * Sample buffer
 * Bacterial lysis
 * 6His purification
 * MBP purification

Bacteria

 * Media
 * Antibiotics
 * Tips on streaking/culturing
 * Freezing procedure
 * Genomic DNA prep (Qiagen)
 * RNA prep (Trizol)
 * ß-Gal/GUS assay
 * LIVE/DEAD BacLight Staining
 * Triparental Mating
 * Transduction
 * Chemocompetent Cells
 * Transformation
 * Electrocompetent Cells
 * Transformation
 * LPS Analysis

Plants

 * Media
 * Conetainer Rack Protocol
 * Seed scarification and germination
 * Tips for growth/seed storage
 * DNA prep
 * RNA prep
 * Seed Storage
 * Microscopy
 * Nodule Bacteria Recovery
 * Nodule Fixation/Embedding
 * LacZ Staining of Nodules