Chang Lab:Notebook/CBE/08/149/2008/12/23

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 * style="background-color: #FFF"|   Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
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 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page


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Logs

 * As per procedure, reading of CV absorbance of Trial IV biofilms

Biofilm Optimization Trial V

 * Same procedure as Trial III, with minor addendum. CONDITIONS: 21 C and 37 C for 72 hrs
 * Addendum: grew 5-hr inoculum using M9 medium, to check (via CV readings) whether there is an effect on biofilm growth.

Sonication step

 * Ultrasonic bath in the lab too strong for biofilm removal.
 * Utilized sonicator probe in Prof Kunn's Lab ==> too weak.
 * Purchase lids with detachable pegs.

Plate count on 1.0 McFarland LB and M9 of Trial IV (12/20)

 * LB:
 * 10^-12: 105
 * 10^-14: 50
 * M9:
 * 10^-16: 359


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