Kubke Lab:/Notebook/Cranial nerve development/2011/01/27

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Cranial Nerve Development
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=General Entries=
 * sorry guys, forgot I was meant to work at Tamaki all day today, but had difficulty connecting to the internet.

=Personal Entries=

Malisha
- I have edited my entry.
 * 9.00 AM- 10.30 AM: Working on journal entries for the 24/1 and 25/1. Prepared RC005 for cutting.
 * 10.30 AM- 11.00 : Discussed with Reuben about cutting RC005 to create sagittal sections.
 * 11.00-12.00: Placed RC005 in the cryostat for incubation at temp CT/OT: -19/-19.
 * 12.00 -1.00 :Placed the OCT mould-embryo onto the chuck .Added more OCT and placed the chuck into the cryostat.
 * 1.00 -2.55 PM: Cut RC005 and finished the whole embryo cutting in a sagittal plane. 9 slides composed of 87 sections with only 1 section lost due to curling.
 * 3.15: Journal entries.

Reuben

 * 10:30am Meeting with Malisha and discussion about cutting RC005 saggitally through the hindbrain.


 * 11:00am Filtering solutions for cresyl violet staining of MH008 including Xylene 1, 100% EtOH 1 and Cresyl Violet.--Reuben Cutfield 17:23, 26 January 2011 (EST)
 * 11:30am Cresyl Violet Stain of MH008.--Reuben Cutfield 19:33, 26 January 2011 (EST)
 * 1:00pm-2:00pm Looking at sections from RC015.

Fabiana

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