Shreffler:Notebook/Basophil Imaging/2010/03/22

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 * style="background-color: #EEE"|[[Image:Baso_confocal.png]] Basophil Imaging
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Call with Alex and Madhan

 * Establish biosafefy approval for live imaging
 * membrane staining, can work out in fixed cells by Confocal
 * Madhan will send beads to Falcone
 * Matt joining project in summer
 * May start with confocal -- reproduce phenotype, play with membrane dyes
 * Will move to live imaging once approval is in place
 * Will consider use of actin polymerization inhibitors down the road
 * Alex will focus on image acquisition, analysis
 * Falcone lab will do TIRF/TIRM
 * Shreffler lab will focus on Ag uptake, cell-cell interactions
 * Wayne will email Dr. Sampson about how best to work out collaboration


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