User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/19

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Qiagen Extraction Tsukuba 3 D4 & E.Windsor C3
~200 individuals Used 1mM NaCl to make rotifers curl, then moved to extraction tube

Followed standard Qiagen tissue extraction protocol

Nanodrop DNA Tsukuba 3 D4 = 6.9 ng/ul E.Windsor C3 = 2.9 ng/ul

HS Takara PCR Optimization Nuclear Gene
Load 2.0ul sample



PCR

 * standard long PCR cycling conditions

Gel



 * Only Avhsp-3_1-1R amplified well, although Avhsp-1_7-2F might work in a larger volume

PCR test (TopTaq)
load 2.5ul of sample



load 2.5ul of sample