User:Anthony Salvagno/Notebook/Research/2009/10/29/Redoing Unzipping: Gel of Redone Anchor PCR

Gel Results


The image is blurry due to fog on the filter. I really should figure out some way to eliminate that. Anyways here is the lane readout: See yesterday for contents of tube It seems like Tube 1 almost worked and Tube 4 definitely worked. Tubes 2 and 3 definitely did not work. What happened in tube 1? Maybe extension is too long. I had this result happen to me before. I definitely think this is a thermal cycler thing now. There is relatively no difference in these reactions outside of the primers, and their locations are at most 100bp apart. I think the reaction worked depending on where I put them in the thermal cycler. I def need to take measurements. Anyways
 * 1) 1x Ladder
 * 2) 2x Ladder
 * 3) Tube 1
 * 4) Tube 2
 * 5) Tube 3
 * 6) Tube 4