WRB080106-Construction of pSB1A2-))))))

[TetR]+[rbs][LuxI][dblTerm] digest pSB1A2-R0040 (Spe, BglI, Neb2, small-991) digest pSB1AK3-F1610 (Xba,BglI, Neb2, large-3058) Ligate, Product is pSB1AK0-Jxxxxx