Template:SBB-Protocols Enz5

Ligation of EIPCR digests
7.5uL ddH2O 1uL T4 DNA Ligase Buffer (small red or black-striped tubes) 1uL PCR product digest 0.5uL T4 DNA Ligase
 * Set up the following reaction:
 * Pound upside down on the bench to mix
 * Give it a quick spin to send it back to the bottom of the tube
 * Incubate on the benchtop for 30min
 * Put on ice and proceed to the transformation