Jessica Karen Wong/Notebook/2007-8-3


 * Sent for sequencing
 * I2056 scarred in 3K3 colonies 4-7, I2057-ES and I2057-EX sealed w/ Not1
 * Each processed with VF and VR primers
 * Started Mutagenesis
 * Did thermal cycling and digests
 * Waiting to transform until we are sure we have competent cells
 * Saw no growth on any of the 10 overnight plates - PCR I2055 sealed and with RBS's, and PCR I2057 with Promoters
 * Replated all of yesterday's transformations in Felix's new batch of Top10
 * Digested presealed I2057-ES and I2057-EX E/S and E/X respectively
 * Used both DNA in EB and in 8.5 water