User:Eric Ma/Notebook/MICB323 Lab Book/2009/03/17

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Experimental Goals

 * 1) Perform an Immunoprecipitation of iNOS/NOS2 from LPS +/- IFNg stimulated cell lysates.
 * 2) Preparation of 8% PAGE running gel.

Part One: IP of iNOS/NOS2

 * Volumes of RIPA buffer required for IP. (See previous entry for calculation of volumes.)
 * I am student A, Sunny is Student B, therefore the samples are divided as follows:

Part Two: 8% Acrylamide Gel

 * Done with no problems encountered.


 * }