User:Fermenter User/Notebook/STRY C2T

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

STRY C2T

Day -1 (Apr 14 Tue):
              4/14/2009 11:20 Bring powder to fermenter room Set up of F6                4/14/2009 11:20 Calibrate pH sensor 4/14/2009 11:20 Make 5-L media 4/14/2009 11:20 Add 1 ml of antifoam 4/14/2009 11:26 Autoclave 4/14/2009 12:40 Cool down bioreactor 800 rpm, 1-L/min air 4/14/2009 12:42 Start software

Set up of F4                4/14/2009 12:00 Calibrate pH sensor 4/14/2009 12:20 Make 50-L media 4/14/2009 12:20 Add 10 ml of antifoam 4/14/2009 12:30 Start software 4/14/2009 12:40 Start Sterilization routine 4/14/2009 14:00 Cool down bioreactor 800 rpm, 50-L/min air

Day 0 (Apr 15 Wed):
-- Set up of F6 --- 4/15/2009 07:00 Connect Base (400 ml) 4/15/2009 07:00 Set pH                          4/15/2009 07:00 Calibrate dO2 probe 4/15/2009 07:00 Set dO2

-- Set up of F4 --- 4/15/2009 07:00 Connect Base (875 ml) 4/15/2009 08:00 Set pH                          4/15/2009 08:00 Calibrate dO2 probe 4/15/2009 08:00 Set dO2

              4/15/2009 11:00  Add antibiotics               4/15/2009 11:00  Fermentation 0:00 Inoculation F4: 1000 ml                                         F6: 10 ml                   4/15/2009 14:30  Fermentation 03:30 Sampling (F4): OD (0.9) Sampling (F6): OD (0.15)            4/15/2009 15:30  Fermentation 04:30 Change temperature            4/15/2009 16:30  Fermentation 05:30  Fermentation 00:00 Add inducer

Day 1 (Apr 16 Thu):
           4/16/2009 07:00 Fermentation 20:00  Fermentation 14:30 F4: Base (600 ml) F6: Base (390 ml)               4/16/2009 10:30 Fermentation 23:30  Fermentation 18:00 F4: OD 2.1 F6: OD 1.4               4/16/2009 15:00 Fermentation 28:00  Fermentation 22:30 F4: OD 2.5 F6: OD 1.5

Day 2 (Apr 17 Fri):
<Sung-Hye>           4/17/2009 07:00 Fermentation 20:00  Fermentation 14:30 F4: Base (580 ml) F6: Base (390 ml) <Frank>              4/17/2009 10:30 Fermentation 23:30  Fermentation 18:00 F4: OD 2.1 F6: OD 1.4 <Sung-Hye>           4/17/2009 13:30  Havest and Finish Fermentation


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