Paulsson:Fluorescent proteins

Brief description of what's out there and what we've got.


 * EYFP (is what people are usually referring to when they say just YFP)
 * GFP with S65G/S72A/T203Y


 * Citrine (Griesbeck JBC'01)
 * EYFP with V68L/Q69M


 * Venus (Nagai NBT'02)
 * EYFP with F46L/F64L/M153T/V163A/S175G


 * Our "Venus" (aka Citrus)
 * Venus with V68L/Q69M


 * Silver's Venus (Surprisingly, approx 8 fold less bright than Citrus when expressed in E.coli; expression may be the problem, rather than brightness)
 * Citrus with V68L / R80Q / H231L


 * Citrus-LAA


 * Citrus-LAA+2 (NY sequence repeated as spacer between ClpX and SspB binding sites)


 * weak RBS - Citrus


 * weak RBS - Citrus-LAA


 * mCherry

Venus with I47L/S208F/V224L/H231E/D234N
 * YPet (Nguyen NBT'05)

What mutations do
F46L: faster maturation at 37deg #Nagai  F64L: reduced sensitivity to pH and Cl-, folding V68L: reduced sensitivity to pH #Miyawaki  Q69M: 2x photostabilty, lower pKa (5.7), indifference to chloride, better expression at 37deg and in organelles #Griesbeck  T203Y: A206K: monomer (instead of weak dimer)


 * 1) Nagai pmid=11753368
 * 2) Miyawaki pmid=10051607
 * 3) Griesbeck pmid=11387331