User:Karmella Haynes/Notebook/miR Trigger/2011/02/25

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02/25/11

 * &#x2713; Transfection: (lipo) miR sensors into JDS33-47

Lipo transfection > JDS33-47: 2 plates

> Add Lipo to 250 μL Opti-MEM --> R.T/ 5 min. > Add DNA to 250 μL Opti-MEM > Add DNA mix to Lipo mix --> R.T./ 20 min. > Add 500 μL complexes to each well (4 ml final volume of medium); Grow cells at 37&deg;C overnight > Refresh ab-free medium the next morning


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