IGEM:Harvard/2007/Meetings/Week 9

[[Media: shlo0813igem.ppt | Stephanie's Powerpoint]]

Lunchtime meeting, 8/17
Harvard 2007 iGEM Jamboree Presentation Planning Meeting 1, 8/17  Content Introduction  Targeting   Cell-cell signaling Intracellular signaling  Conclusion
 * How cells physically interact with environment?
 * Underutilized aspects of iGEM: random libraries, surface expression
 * Figure of overall project: "Harvard iGEM 2007"; overall fusion of project
 * Random library
 * Loop/terminal
 * Targets
 * Nickel
 * Calmodulin
 * EGF
 * GST
 * His
 * Strep
 * PDZ
 * Figures: Plates: before and after enrichment, somehow present numbers (pie chart? bar graph?), microscopy images with beads and fluorescent cells accumulating
 * QS – sender, receiver = biobricks, back to initial idea: target + QS, with figures
 * Figures: drop experiment (halo)
 * Advantage of Fec system = gene expression with low level of background – need specificity with targets, etc, regulation on cell level rather than on population level
 * Figures: 3d structure, where and why we chose to alter loop region
 * “Big goals” – medical applications
 * Self-regulated production of certain proteins – “preprogrammed assembly”
 * Constructs / futures

SLIDE allotment
 * allow 45 seconds or so per slide
 * total of 15 ish slides
 * Intro = 2 slides
 * Slide 1: Why do we want to do this?
 * Slide 2: This is how we're going to do it; architecture of project
 * Targeting = 4
 * Cell-cell (QS) = 4
 * Intracellular signaling = 3
 * Conclusion = 2

TIME allotment
 * Intro: 1 min
 * Targeting: 3 min
 * Cell-cell signaling: 2.5 min
 * Intracellular signaling: 2.5 min
 * Conclusion: 1 min