User:Anthony Salvagno/Notebook/Research/2010/01/25/Jasper PCR Again

Try Number 3
Ok I discovered this morning that I just simply wasn't putting in enough primer. So before doing too much tampering, I will just add more primer. I will also raise the temp of the reaction to 64C. If this doesn't work, then I will try the 1.1kb PCR from SDM and also try a Mg++ molarity gradient. But first things first.

Gel Results
Ok this didn't work and it looks weird. I don't get why the bands are brighter in the 1st four lanes then the second four. Here is the lane designations: So having brighter bands means the concentrations isn't what it is supposed to be? I suppose it could also mean that Cy3 fluoresces at the wavelength of Sybr Safe, I don't know. I really have no clue what is going on.
 * 1) 100bp ladder
 * 2) tube 1
 * 3) tube 2
 * 4) tube 3
 * 5) tube 4
 * 6) tube a
 * 7) tube b
 * 8) tube c
 * 9) tube d