User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/05/20

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Maize Endophyte Biofertilizers
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The Daily Grind

 * Actually doing that PCR today of all the endophytes.
 * Met with Manish and he was OK on the TRFLP using the new nested primers.
 * None of the electroporations with Arabad::GFP worked, not even the positive (E. coli) control. Will have to try again.
 * Setting 20 seeds of B73, Gaspe, Pioneer, and Mixteco to germinate for greenhouse innoculation experiment. They will be innoculated with 100 mL of phosphate buffer with 0D600=0.2 Burkholderia phytofirmans from Mixteco (2A10), Pantoea agglomerans from B73 (1A1), or sterile phosphate buffer


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