IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-14

CIP treatment (fail)
CIP treated the '0S' sequence, which is J36010/SP. Will need to re-CIP treat.

Gel extraction



 * 1: Ladder
 * 2: undigested 36010
 * 3: digested 36010 /XP marker
 * 4: digested 36010 /XP
 * 5: digested 36010 /XP
 * 6: digested 36010 /XP (not shown)
 * 7: digested 36010 /XP (not shown)
 * 8: digested 36010 /XP marker
 * 9: Ladder
 * 10: digested 36011 /SP
 * 11: digested 36011 /SP (not shown)
 * 12: Ladder
 * 13: digested 36010 /SP marker
 * 14: digested 36010 /SP
 * 15: digested 36010 /SP
 * 16: digested 36010 /SP
 * 17: digested 36010 /SP
 * 18: digested 36010 /SP marker
 * 19: undigested 36010
 * 20: Ladder

The gel ran as if there was a lot of genomic DNA; additionally there is no KaiC /XP expected 1.8kb insert in lanes 3-5; and notice in lane 10 that the supposed KaiB /SP is a smudge.

Will poststain next gel to verify results.

Gel#2:




 * 1: ladder
 * 2: undigested 36010
 * 3: undigested 36011
 * 4: digested 36011 /sp marker
 * 5: digested 36011 /sp (not shown)
 * 6: digested 36011 /sp (not shown)
 * 7 digested 36011 /sp marker
 * 8: ladder

Innoculation
Reinnoculated M27 and B18.