User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/10

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04/10/10

 * &#x2713; Assemblies: re-digest vectors and KAH96 from yesterday; re-do ligations

Assemblies --> See 4/09/10

> Digests (Fermentas FD) --> Repeat digests from yesterday; increase time to 1 hour:
 * 1) KAH96 (E/S/ApaLI)
 * 2) KAH145 (E/X)
 * 3) KAH116 (E/X)

> Measure conc.'s

> Ligations

--> R.T./ ~10 min.; Add to 20 μL Z-DH5α

--> 4/11/10: Success! Pick 2 colonies each.


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