Berglund:List of Antibodies Available

Primary Antibodies His-probe (G-18; anti-rabbit) Antibody (bought Jan, 2010)(Voelker) sc-804; Santa Cruz Biotechnology; dilution range 1:100-1:1000); 200µg/1ml- for immuno precipitation use 1-2µg/100-500µg of total protein (1ml cell lysate) blocking peptide can be bought sc-804 P for competition studies HuR(3A2) Antibody (bought 2009)200 ug/mL; sc-5261; Santa Cruz Biotechnology (Purcell) monoclonal antibody HA.11 (Covance, MMS-101R, purchased 2008) FRG1 (L-07) mouse monoclonal antibody 50ug/0.5mL;(sc-101050, Santa Cruz, purchased 2008) Raised anti-SF1 (not completely characterized) anti-U2AF65 Santa Cruz (purchased 2007, sc-48804) used at 1:200, dilution range is 1:100-1:1000 anti-actin Santa Cruz (purchased 2007, sc-1616) used at 1:200, dilution range is 1:100-1:1000 anti-HNRNP E2 (H-15) Santa Cruz (purchased 2007, sc-30725) used at 1:200, dilution range is 1:100-1:1000 anti-MBL (made 2007-rabbit) anti-MBNL (made 2007-rabbit) 2,27-trimethylguanosine (Ab-1) monoclonal antibody to nucleic acid (purchased 2006?, oncogene, Na02, NA02A). This antibody reacts with the exposed 5' cap structure of the snRNAs and can be used for purification of snRNPs by immunoaffinity chromatography. anti-dig ap fab, fragments for in situ hyb, cat# 11093274910, Roche, bought 10/5/2010 HuR(3A2) AntibodySanta Cruz Biotechnology, cat # sc-5261, 200 ug/mL, bought 1/29/2009

Secondary Antibodies

donkey anti-goat Santa Cruz (purchased 2007) bovine anti-mouse Santa Cruz (purchased 200??) goat anti-rabbit Santa Cruz (purchased 2007) goat anti-chicken (Jackson ImmunoResearch, Alkaline phosphatase conjugated, 103-055-155) rabbit anti-chicken (Jackson ImmunoResearch, peroxidase-conjugated, 303-035-003) rabbit anti-chicken (Jackson ImmunoResearch, peroxidase-conjugated, 303-035-008)

Blocking serums: Normal chicken serum (Jackson ImmunoResearch, 003-000-120)