Registry/Measurement kit/Notebook/2007-6-29

To do

 * 1) Colony PCR of promoter tester (We got 3 colonies (1AT3))
 * 2) Re-try colony PCR of RBS tester
 * 3) Run them on a gel
 * 4) MfeI/NsiI digest of E0240
 * 5) PCR cleanup E0240
 * 6) Ligate E0240 and 3K3, transform

Colony PCR

 * Trying extension time of 1:45, in case 1:30 was too short
 * Made new dNTP dilution (108 H2O + 3 of each NTP)

Analytic Gel

 * Loading bottom row:
 * Lanes 1-5 and 16-20 empty or ladder; lanes 6-12 rbs tester; lanes 13-15 promoter tester.

Digest

 * Can't digest with both enzymes concurrently according to NEB, so cutting with MfeI first