Jessica Karen Wong/Notebook/2007-7-24

I2056

 * Ran a gel of the overnight PCR
 * Looks good
 * Digesting with Mfe and Nsi in buffer 2 overnight

T9002

 * Got new T9002_E0240_R primer in - diluted to 40 uM
 * Set up a 100ul supermix PCR rxn of E0240 for T9002 to put on tails
 * Ran at 52
 * Ran a gel which looks great
 * PCR cleaned
 * Digesting w/ Spe/Nsi in buffer 2 overnight

Other

 * Made a new overnight from the registry plate of 1AK3
 * Got sequencing back for I2055-3K3 - really wierd
 * 1st half of part (till middle of GFP) was put in backwards, rest of part was forwards
 * Found that I2055 has an Mfe1 site in the middle of GFP and that's where the problem happened
 * Changed synthesis proposal so doesn't have the Mfe1 site in GFP - silent mutation