IGEM:MIT/2007/Notebook/2007-7-25

Agenda

 * 1) Redo transformation of merR/Pmer+I13500
 * 2) *Plates in @3:25pm
 * 3) Make LC of last night's CPX and FhuA transformations into BL21 (eventually will make glycerol stocks)
 * 4) Run western blot on CPX trans. cells (check for protein expression)
 * 5) Run assay on FhuA trans. cells (test poly-styrene binding)

Polystyrene Assay
Gr

merR/Pmer+I13500 Transformation Results
Plate             # colonies     Notes - control         0 merR/Pmer+I13500  0              :( + cotrol (pUC18)   smear

BL21 Transformation Results
Plate                   # Colonies       Notes FhuA (pICCBP)           38               Colonies are differently sized pUC18                   15               2 or 3 are much smaller F+B+CPX (colony PCR #6) ~200

Second merR/Pmer+I13500 Transformation

 * Spun down unplated remnants of transformation. Saw a VERY small pellet.
 * Resuspended spun-down cells and plated all of those.

LC of BL21's trans. with F+B+CPX and pICCSP

 * went in around 4PM