IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/28

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Test AmyE insertion

 * Colonies PCR for Bacillus (IA751 transformed with ECE153, IA751, and IA771)

- 20μL of lysozyme, a few colonies for each

- Cycle : 15min qt 37°C, 15min at 99°C, 1min at 4°C, 1min at 99°C and 1min at 4°C

- for each PCR : 12μL of cells, 1μL of cells, 1μL of primer1, 1μL of primer2, 5μL of master mix

- PCR (iGEM program)

- run a gel




 * Result : nothing on the gel!!! But we can not manage to PCR anything... there is a problem in our PCR protocol, or products!


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