Springer Lab: YNB media

Back to Springer Lab

Yeast Nitrogen Base (YNB), per liter

 * Biotin 2 &mu;g
 * Calcium pantothenate 400 &mu;g
 * Folic acid 2 &mu;g
 * Inositol 2000 &mu;g
 * Niacin 400 &mu;g
 * p-Aminobenzoic acid 200 &mu;g
 * Pyridoxine hydrochloride 400 &mu;g
 * Riboflavin 200 &mu;g
 * Thiamine hydrochloride 400 &mu;g


 * Boric acid 500 &mu;g
 * Copper sulfate 40 &mu;g
 * Potassium iodide 100 &mu;g
 * Ferric chloride 200 &mu;g
 * Manganese sulfate 400 &mu;g
 * Sodium molybdate 200 &mu;g
 * Zinc sulfate 400 &mu;g


 * Potassium phosphate monobasic 1 g
 * Magnesium sulfate 500 mg
 * Sodium chloride 100 mg
 * Calcium chloride 100 mg


 * Final pH 4.5 before addition of Ammonium sulfate (5 g/liter)
 * Final pH 5.4 after addition of Ammonium sulfate (5 g/liter)


 * Difco 0919-15 Yeast nitrogen base w/o amino acids  100g
 * Difco 0335-15 Yeast nitrogen base w/o amino acids and ammonium sulfate 100g