Template:SBB10 ConstructionFiles JoseG sbb38

Biobricking of Pbad PCR JG003-F and JG004-R on pBca1100-Bca1152. (276 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144                     (EcoRI/BamHI, 910+2170, L) Product is Pbad-pBjk2741    {Pbad-J23100} JG003-F  Cloning of papC   ccataGAATTCgtccAGATCTaaccaattgtccatattgcatcag JG004-R  Cloning of papC   ctgatGGATCCgctaatcttatggataaaaatgc 

JCA Notes
Eco/Bam transfer pBjh1601CK-ig114 Sub into pBjk2741-Bca1144                     (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-ig114   {AraC-Pbad}
 * This was a little misleading, we actually want AraC-Pbad, not just the pbad promoter itself. This construction file would adequately make a Pbad-only part
 * Nomenclature of plasmid changed
 * Modified entry on clotho