IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/26

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7/26/10
9:50 AM

Made glycerol stocks of 5 mL cultures

ArsR SDM1 ISB206

ArsR SDM2 ISB207

Miniprep (promega kit)

ArsR SDM1 184.9 ng/μL

ArsR SDM2 209.3 ng/μL

12:15 PM

PCR

Per Reaction: 19.2 μL mastermix, .4 μL F primer, .4 μL R primer.

Samples

LamBF1R

LamBF2R

ArsB

Negative control (no primers)

Primers

All primers at 10 micromolar

LamBF1

LamBF2

LamBR

ArsBF2

ArsBR2

Program

3:30 PM

Gel

1% TAE, 175V




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