DNA Precipitation protocol

Solutions/reagents: 3M Sodium Acetate solutionGlycogen95% EtOH70% EtOHwaterbufferDNA sample Equipment: Centrifuge</li></ul> Steps: <ol> Measure out <font color=#357EC7>0.1  volume <font color=#357EC7>3M Sodium Acetate solution into DNA sample. </li> Measure out <font color=#357EC7>1 µl  of <font color=#357EC7>Glycogen into Eppendorf tube (1). </li> Add  <font color=#357EC7>2  volumes <font color=#357EC7>95% EtOH. </li>  Option 1: Store at <font color=#357EC7>-20°C  for <font color=#357EC7>12 hrs (overnight). (or) Option 2: Store at <font color=#357EC7>-80°C  for <font color=#357EC7>30 mins . </li> Centrifuge at <font color=#357EC7>maximum speed for at least <font color=#357EC7>15 mins  at <font color=#357EC7>room temperature , gently aspirate out the supernatant and discard it. </li>  (Optional)  Add <font color=#357EC7>1 ml  of <font color=#357EC7>70% EtOH. Store at <font color=#357EC7><b><font color=#357EC7>room temperature  </b> for <font color=#357EC7>5 mins . </li>  (Optional)  Centrifuge at <font color=#357EC7>maximum speed for <font color=#357EC7>5 mins  at <font color=#357EC7>room temperature , gently aspirate out the supernatant and discard it. </li> Dry the pellet in air for <font color=#357EC7>10 - 15 mins . <font color = "#800517">Dry until all the liquid is gone. </li>  Option 1: Add <font color=#357EC7>water to pellet. (or) Option 2: Add <font color=#357EC7>buffer to water. Resuspend pellet by vortexing/by shaking vigorously. </li> </ol> TOTAL TIME REQUIRED FOR THE COMPLETION OF THE PROTOCOL :<font color=#357EC7>~ 12 hrs, 25 mins