Shreffler:Notebook/Staff Meetings/2010/08/17

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] ShreffLab Staff Meetings
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Agenda Items

 * EoE
 * EDN ELISAs
 * Sarita has ordered a kit to try? and will initially evaluate with stds from company?
 * Conference call with Mirna on Friday to discuss possible collaboration and availability of samples for validation
 * Component proposal -- WS still needs to work on
 * Basophil
 * Review data from last week
 * non-responder -- x2
 * will implement a 'pre-screen' protocol to avoid basophil isolation and Derp2 specific sensitization with bad donors
 * will run sups for cytokine (CBA) from first 2 exps presuming responder sample obtained this week
 * Review ALK data
 * -- non-responder -- will not save sups
 * Histamine data
 * Background is ~15% -- why so high? donor is non-responder
 * Next experiments
 * Repeat with buffy's this week


 * Allergen Adjuvant
 * 2D done by Galina
 * confirms pI ~10
 * Bert will arrange to have gel sent to Hazmik
 * Foreskin DC flow data -- looked good
 * Thu cells to characterize, Fri Aldefluor
 * Flow Thu 5-7p
 * mAb -- how will we approach
 * PM Baso
 * no samples last week
 * JAX
 * quote for peptide arrays
 * WS has to do
 * train Stephanie for project role
 * Stephanie almost self-sufficient now
 * Carboplatin
 * no samples
 * Isabelle's project -- multi-lingual
 * Action plan available in Spanish, will get Portuguese version
 * Has 30 pages of recipes compiled
 * U19
 * Set up PBMC this week to milk allergen
 * Bert will try to identify allergen source and obtain endotoxin kit

Other Discussion

 * Hood certification/ CO2 tanks
 * will take over cost of that room

Things To Do

 * 1) Write up component proposal (Marcella and Wayne)
 * 2) obtain quote for peptide arrays
 * 3) send Bert U19 slides