IGEM:MIT/2006/Notebook/2006-6-17

am

 * 1) check BL21 plates for transformants -- TONS of transformants! They were borderline overgrown, even in the 20&mu;L plates.  I put them in the fridge; I will start liquid cultures sometime this afternoon.
 * 2) make D, E, F liquid cultures in LB Kan from each plate (assuming possible) -- Did this at 8PM; I chose 3 colonies from each of the 3 plates (20&mu;L BAMT, SAMT, and BSMT), and cultured them in 9 tubes. I put them near the center of the rotating tube holder in the 37 degree room (for whoever comes in tomorrow...they're labeled with what they contain + VV + 6/17...it's hard to miss them :))
 * 3) put in 37c room with shaking for about 16 hours -- so...noon-ish sunday
 * 4) will remove next am