Template:SBB10 ConstructionFiles JennaK sbb42

Biobricking of pre-pro sequence basic part sbb42

Digest pBjh1601AC-Bjh1723                     (BglII/BamHI, 91+3203, S) Sub into pBca9523-Bca1144                      (EcoRI/BamHI, 918+2472, L) Product is pBca9523-rbs_pelB  	{rbs_pelB>}



JCA Notes
Eco/Bam transfer pBjh1601AC-Bjh1723 Sub into pBjk2741-Bca1144           (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-Bjh1723  	{rbs_pelB>}
 * You're going to be digesting EcoRI/BamHI for both source and destination
 * You want to transfer into pBjk2471
 * Product has same partName as source