User:Burak Yilmaz/Notebook/YR10 protein interaction/2009/09/22

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plasmid concentrator

 * using zymo kit

Protocol 300 μl DNA Binding Buffer to 150 μl DNA sample). Transfer sample mixtures to the wells of a Zymo-Spin I-96 Plate™ mounted on a Collection Plate. Alternatively, add two volumes of DNA Binding Buffer to each volume of DNA sample directly in the wells of the Zymo-Spin I-96 Plate™ mounted on a Collection Plate. Ensure proper mixing by pipetting up and down a few times. mixtures have been completely filtered. Discard the flow-through. Centrifuge at 2,500-8,000 rpm for 5 minutes. Repeat wash step. (Alternatively, one wash can be performed using 600 μl Wash Buffer). Zymo-Spin I-96 Plate™ onto an Elution Plate and centrifuge at 2,500-8,000 rpm for 5 minutes to elute the DNA. Ultra-pure DNA in water is now ready for use.
 * 1. Add two volumes of DNA Binding Buffer to each volume of DNA sample. (e.g.,
 * 2. Centrifuge at 2,500-8,000 rpm (minimum 1000 x g) for 5 minutes until sample
 * 3. Add 300 μl Wash Buffer to each well of the Zymo-Spin I-96 Plate™.
 * 4. Add 10-15 μl water directly to the column matrix in each well. Transfer the


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