User:Meng Xiao He/Notebook/fall08/2008/11/04

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Test digest w/ SmaI



 * left most: pUC19 digest, control (2686)
 * next 3: cbb3+pUC19
 * 1kb ladder
 * next 2: cco+pUC19
 * 100bp ladder
 * next 3: Duo+pUC19
 * lane 12 is good> it should contain the Duo flank in pUC19's XmaI site

New transformations

 * TOPO things into TOP10
 * pER21 (+0, cbb3, cco, Duo) into chem pir-116


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