Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 3

MACS with strep and his plus cherry/red bacteria (07/02/07)
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Run the MACS with the cherry/red bacteria incorporated to see if separation occurs (07/03/07)

 * 1) Followed Indirect Magnetic Labeling Protocol
 * 2) Used 300 ul of Strep/His and 300 ul of RFP Bacteria
 * 3) Note: OMPA1+Strep and OMPA1+His had low cell count
 * 4) Then follow Magnetic Separation

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Results from MACS plate
Observation: Still have some background in the elution fraction, instead of the plain white strep and his colonies that we expected.

Conclusion: We may need to further optimize the wash steps, because some of the extraneous bacteria expected to be gone appear in the elution fraction.

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Transformation of BL21DE3 RFP cells (07/05/07)

 * For use in MACS assay.