Matt Gethers/CRI, Thailand/Labwork/Isolating HmgR/Week of 6.8.08

=6.11.08=

Primer Design for HmgR ORF
Here is the KEGG File for HmgR (pa2010). I'd like to amplify this ORF and then drop it into the pET-11a commercial vector between the NdeI and BamHI sites. The ORF has to be in frame and I have to be sure there aren't any cloning sites in the ORF.

Using the file of HmgR from Mayuree

Forward Primer BT2736 (5' to 3')
Specs

LENGTH (annealing/all): 21/27

GC CONTENT (not including non-anealing bases):47.6 %

MELT TEMP (not including non-anealing bases): 55.4 ºC

Min(ΔG): -0.07 kcal.mole-1

Reverse Primer BT2737 (5' to 3')
Specs

LENGTH (annealing/all): 18/27

GC CONTENT (not including non-anealing bases): 61.1 %

MELT TEMP (not including non-annealing bases): 57.4 ºC

Min(ΔG): -4.01 kcal.mole-1