Luckau Protocols:Tris-Cl

Purpose

 * Tris-Cl is a buffering agent (made from Tris base and HCl)
 * We use it as a stock solution for Low TE

Info

 * tris [hydroxymethyl] amino methane (C4H11NO3, 2-Amino-2-hydroxymethyl-propane-1,3-diol)
 * MW = 157.56 g/mol (MW = molecular weight, same as formula weight, FW)

Calculations

 * $$500 \mathrm{mL} \bullet \tfrac{0.5 \mathrm{mol}}{\mathrm{L}}\ \bullet \tfrac{\mathrm{L}}{1000 \mathrm{mL}}\ \bullet \tfrac{157.56 \mathrm{g}}{\mathrm{mol}}\ = 39.39 \mathrm{g}$$


 * $$500 \mathrm{mL} \bullet \tfrac{1 \mathrm{mol}}{\mathrm{L}}\ \bullet \tfrac{\mathrm{L}}{1000 \mathrm{mL}}\ \bullet \tfrac{157.56 \mathrm{g}}{\mathrm{mol}}\ = 78.78 \mathrm{g}$$

To make $$\tfrac{1}{2}$$ liter of 0.5M or 1M Tris-Cl, pH 8.0

 * 1) In 1 L beaker, mix Tris-Cl in 400 mL H2O
 * 2) Put beaker on hotplate with stirbar until dissolved
 * 3) Using concentrated aqueous HCl to adjust to pH 8.0
 * 4) Add H2O to bring volume to 500 mL
 * 5) Autoclave solution, store at room temperature