Escherichia coli/Vectors

Stringent vs. relaxed replication
Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the abscence of protein production - stringent control (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed relaxed control. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--BC 19:05, 3 Sep 2005 (EDT)

Nomenclature

 * 1) Novick-BacteriolRev-1976 pmid=1267736

Replicon compatibility
Replication origins in different incompatibility groups are compatible. Replication origins in the same incompatibility group are not.

Some sets of vectors with compatible origins are available as a part of the Novagen Duet system. (from TK)

Individual vector links
''Note: searching for cloning vector when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.''


 * pUC19
 * pBeloBAC11
 * pBACe3.6
 * F plasmid
 * pSCANS genbank vector info cookbook
 * pSC101 replication origin
 * pBR322
 * pACYC184