Klapperich Lab:Notebook/Lab Meeting Notes/2008/11/11

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11 November 2008 Lab Meeting Agenda
† Hussam's research report today.

† Announcements † Flu R01 † SEPSIS Project - CIMIT Bird Flu †RNA project † COBRA † Valve Array/Valve building (FJ) † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR '''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ''' † RCA † Senior project † F31: Cochlea † Silica Optimization (Lambda):
 * Your personal priorities have been emailed to you by me. Please take note.
 * Courier service! Sample transport set up.
 * Plaque assay status -- plaques and everything!Yeah!
 * Mark - status of fixture?
 * Cathie working on paper.
 * Jeff Braman will visit in early Dec.
 * Do cDNA prep. Spec, gels, send to Jeff B. Shipping?
 * Mark- Control circuit?
 * Bacteria Experiment - Changing the teflon filter.
 * Pluronic results? - straight channels. Treated/non, pluronic better. NEED UPDATE FROM JD
 * Substrates - JZ tried with more gold and do all SEM/sample testing.
 * Status from Mark? - valves in transit somewhere.
 * Frank should talk with Alex, Mark, and Jaephil and make sure we are not duplicating efforts/making something that is too big for portability.
 * Frank met with Mark on Friday, has concerns along the lines of the above comment (duplicating efforts etc.) that will hopefully be addressed during/after lab meeting.
 * Primer/dimer issue. Talked with QQ. We do not appear to have this problem with our chips.
 * Mask made. Chrome mask.
 * Preliminary E. coli experiment is ongoing.
 * Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
 * STAR-CD Renewal - January ($2K)
 * Cathie look up her flu assay specs.
 * Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers.
 * Look into Melting temp analysis on the 7300 - can only do with Sonali's assay.
 * Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit.
 * MinCheol's designs. Done. Data looks the same as old design. Extension time not long enough.
 * Will do extension design with lambda phage assay.
 * Mincheol will work on DNA migration simulation after IMECE 2008.
 * Cathie submitted One pager Whitepaper. Waiting for response.
 * Megan has mask designs, JD will check, send out this week.
 * Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume.
 * Mark will help/learn SU8 step.
 * Teddy doing off chip controls work. Will consult with Megan and JD to get interface design going. Software control, too..LabVIEW.
 * Valves in the mail --- find them! (MARK)
 * Made changes and gsve to Cathie.
 * De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns.
 * Ongoing. More concentrations.
 * Pico Green assay figured out.
 * Ethanol precipitation protocol and preparation of sodium acetate. ASAP.


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