User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/02

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Preparing TRIS Urea gel
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * 8% AA Tris-Urea gel was prepared (separating)
 * Tris pH 8.8 2.5 mL (Should be 1.5 M? But 0.75 M was used)
 * 4.8 g Urea
 * 2.7 mL 30% bis-Acrylamide
 * 100 μL 10% APS
 * 6 μL TEMED
 * Up to 10 mL with H2O (about 1 mL?)
 * 5% AA Tris-Urea gel was prepared (Stacking)
 * Tris pH 6.8 630 μL (Should be 1 M? But 0.625 M was used)
 * 2.4 g Urea
 * 830 μL 30% bis-Acrylamide
 * 50 μL 10% APS
 * 5 μL TEMED
 * Up to 5 mL with H2O (about 2.5 mL?)

Discussion

 * Does the Tris concentration hurt the gel??


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