IGEM:Groningen/2009/Notebook/iGEM 2011/2011/07/06

{| width="800"
 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Entry title
HybB promotor in pSB1CR HybB promotor in RBS GFP ampicillin resistance vector PcI promotor in RBS GFP ampicillin resistance vector PlasI promotor in RBS GFP ampicillin resistance vector LasI-LVA in ampicillin resistance with double terminator vector cI-LVA in ampicillin resistance with double terminator vector
 * Colony PCR of 6 constructs!

Colony PCR protocol: [[Media: Taq PCR mastermix]] Preheated Lid: 111° C Denaturation: 95 ° C for ten minutes Cycle 33× Denaturation: 95° C for 30 seconds Annealing: 60° C for 30 seconds Extension: 72° C for 1 minute Final extension: 72° C for ten minutes Store at 4 ° C (infinite)


 * }