Richard Lab:IC overview

Overview

 * Ion chromatograph with 2 independent pump and column systems and shared autosampler
 * Carbohydrate analysis using CarboPac PA20 columns with pulsed amperometry (Au working electrode)
 * Alcohol and organic acid analysis using ICE-AS1 columns with pulsed amperometry (Pt working electrode) and UV detection

Component Specifications

 * Chromeleon Chromatography Management Software v. 6.8
 * AS autosampler
 * 100-1.5mL vial capacity
 * 250μL needle, 10μL injection loops
 * Diverter valve for sequential (independent) control of dual column/pump system
 * DC detector compartment
 * 15-40°C temperature control
 * Houses 2 ea. injection valves, columns, and ED detectors
 * ED electrochemical detector
 * Combination pH and Ag/AgCl reference electrodes
 * Disposable Au or Pt working electrodes
 * UVD340U UV/Vis detector
 * Simultaneous detection at 4 wavelengths (200-595nm)
 * DP dual pump
 * 2 gradient pumps with integrated vacuum degasser
 * PEEK pump heads and flow path
 * Shodex-101 refractive index detector (RID)
 * Not currently in use

Column Specifications

 * CarboPac PA20
 * 3 x 30mm guard column
 * 3 x 150mm analytical column
 * Borate trap
 * ICE-AS1
 * 4 x 50mm guard column
 * 4 x 250mm analytical column
 * 9 x 250mm analytical column
 * CarboPac MA1 (not currently in use)

Sample Preparation

 * Possible sample neutralization or clean-up
 * Filtration with 0.2μM nylon or PTFE filters
 * Dilution to existing standard curve range
 * 1-40 mg/L sugars
 * 25-200 mg/L alcohols and organic acids

Contact

 * Who has experience with this protocol?

Back to Protocols