IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Prey Construct/Modelling

 Specifications Design Modelling Implementation Testing/Validation  

Model assumptions and relevance

 * LuxR is at steady state
 * A good approximation if LuxR gene is left in an active state while cells are growing in medium
 * LuxR and AHL bind in a linear second order fashion
 * This is not strictly true as LuxR and AHL bind using pseudo-Michaelis-Menten kinetics
 * Gives a good approximation if LuxR is in excess of AHL (ie not saturated by AHL)
 * Unlikely to occur all the time as LuxI levels increase to very high amounts
 * LuxI can be ignored
 * LuxI levels vary throughout, and it is these levels that are directly responsible for AHL production
 * This could be a major flaw in the model

Model description


J37015 SBML File