Koeris/Notebook/2007-1-17

=Thoughts on biofilms= Linares et al. =Transformations= Standard heat shock protocol usable for any kind of origin. Also good for multi-plasmid transformations

Reagents necessary

 * Chemically competent cells (see here)
 * Plasmid prep >5ng/ul concentration as the lower limit is acceptable
 * SOC

Protocol

 * 1) Thaw competent cells on ice
 * 2) Add plasmid DNA to cell, stir with pipette tip, do NOT pipette up and down
 * 3) Incubate for 30min on ice
 * 4) Heat shock at 42 deg C for 30s
 * 5) Cool on ice for 5min
 * 6) Add 1ml SOC to 100ul competent cells
 * 7) Incubate at 37 deg C for 1h at 300rpm
 * 8) Spread plate 550ul on LB agar with the appropriate resistance markers