Griffin:Antibody Elution Buffers

Elution buffers are essential in order to affinity purify antibodies. There are several out there, and below are a representation of 4 very good ones.


 * 100mM Glycine, pH2.5 (acidic pH)


 * 1M Triethanolamine; TEA (basic pH)


 * 4M MgCl2 (high salt)


 * 1M NaCl/PBS (high salt)

In general high salt concentration or extreme pH will disrupt the antibody-protein binding. The optimal elution buffer to use will have to be determined experimentally by the user.