User:Linh N Le/Notebook/2009/07/13

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To Do

 * Autoclave stuff today!
 * Pipette tips (1000ul) that fell out, they are sitting in the autoclave
 * More centrifuge tubes (the large ones)
 * Popin buffer
 * More BNC Cables
 * OT?

Autoclaving

 * When I came in, the Autoclave was in use
 * Andy says that Nathan (Osinski's Lab) was using it
 * Round one (probably the only round since our group meeting is today)
 * 1x 1000ul tips (the ones i got dirty Friday)
 * 2x 100ul tips
 * 5x large centrifuge tips
 * ''Note: I went ahead and autoclaved ALL of the tubes in the plastic bag since we only had 1 more container of them and I wanted to make sure we had some in reserve
 * Round 2
 * The "popin buffer"

BNC

 * Whilst waiting for the Autoclave, I will make some 3ft and 4 ft BNC cables
 * one 4ft made friday, one made today
 * two 3ft made today

New Lab Network

 * Used Koch's laptop to check the 4 ports that are in the new lab (2 at front door 2 at rear door)
 * They all provided me with internet access and I was able to access //controller
 * I will take down the annoying wire tomorrow since today I need to read up on the paper for the meeting

OT

 * Koch, Ant and I tethered some DNA and tried unzipping
 * There is a problem with the translator/piezzo
 * They seem to be misaligned because we get alot of "sliding"
 * Ex. the x translation works fine, but when you use the Y translator it has a diagonal movement wrt the microscope plane
 * We were able to find some tethers and pull on them
 * We feel that the trap is too weak since the beads keep coming out of the trap
 * it could also be a problem with the piezzo (misaligned and/or moving too fast)
 * Good note: 500nm beads are easy to see and very easily trapped (sometimes we get them when we dont want them)
 * We need to calibrate the tweezers tomorrow
 * Cool note: I think i saw dna unzip, but that result was unrepeatable
 * We turned on the trap near a tether, saw it move, then turned off the trap.
 * The bead "sprung back" to its original spot and oscillated a bit (like a block on a spring)


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