User:Tk/Notebook/MF/2009/11/22

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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 * style="background-color: #F2F2F2" align="center"|  |Main Page


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Cutting DNA with Sau3AI and SpeI


Cut 1 μg DNA in 50 μl in buffer 2, BSA, 1 μl enzyme


 * L1
 * Uncut
 * Sau3AI digest
 * SpeI digest


 * GF
 * Uncut
 * Sau3AI digest
 * SpeI digest


 * PP
 * Uncut
 * Sau3AI digest


 * Ent
 * Uncut


 * PP
 * SpeI


 * Ent
 * SpeI digest
 * Sau3AI digest

L1 cannot be cut with Sau3AI. Poorly cut with SpeI. Other strains are completely cut with Sau3AI, cut similarly with SpeI.


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