User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/27

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Heather performed the following on the dissected experimental and control testis:


 * Stain with anti-vis/achi 1:5000 O/N
 * Stain with goat anti-guinea pig 1:500 488 nm for 2 hrs
 * Stain with ADL67.10 anti-lamin from DSHB 1:150
 * Stain with goat anti-mouse 633nm 1:500 for 2 hrs.


 * Antibody dilutions were made in 2% NGS
 * Between each staining, testis were washed 5 x 10' in PBS-T


 * After wash, leave at 4C over weekend, mount on Monday.


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