Lissa1: July9-July16

Monday, July 10

 * 1) Do colony PCR of new pGEV colonies. -DECIDED NOT TO DO
 * 2) Is this worth it? There are only 2 colonies, 1 contamination, and the diagnostic digest looked the same as last time.
 * 3) Look at suspicious cells under microscope to see what they are. -DONE
 * 4) Use Trp1_A and _D to PCR the yeast genome to see if it works. -DONE
 * 5) Where is the yeast genome? -FOUND
 * 6) Run gel of PCR. -DONE
 * 7) Start using Fus3 antibody... -DONE
 * 8) Make alpha-arrested samples - DONE
 * 9) Order anti-goat antibody -DONE
 * 10) Do ImageQuant stuff -DONE

Tuesday, July 11

 * 1) Prep proteins (alpha, beta, etc) - DONE
 * 2) Pour mini small gel (x2) and load it -DONE, run it -DONE, transfer it -DONE, set up incubation -DONE
 * 3) Do transfer -DONE and set up overnight incubation of big Western -DONE
 * 4) Pour new SUMO gel - DONE
 * 5) Set up 2 overnight cultures- DONE

Wednesday, July 12

 * 1) Wash and image big Western *and* little Western - DONE
 * 2) Colony PCR with Trp1_A and Trp1_D of s288c yeast and run gel. -DONE
 * 3) Prep and load unarrested large PPL samples and run for a LONG TIME - SAMPLES DID NOT GROW!!!

Thursday, July 13
RELAY FOR LIFE

Friday, July 14
RELAY FOR LIFE

Saturday, July 15
RELAY FOR LIFE