SBB09 Oligos

Put your oligo sequences into this page. Separate each column with tabs: Ost001F	Forward oligo for upaG	ccaaaGAATTCatgAGATCTgttgagatggataacaaactg Ost002R	Reverse oligo for upaG	ctagcGGATCCttaCCACtgaataccggcaccgag Ost003F	Forward construction for HydroxyapatiteBP	ccaaaGAATTCatgAGATCTgcgccgtggcatctgagcagccagtatag Ost004R	Reverse construction for HydroxyapatiteBP	ctagcGGATCCggtgcggctatactggctgctcagatgc Ost005F	Forward oligo for Beta Helix Repeats	ccaaaGAATTCatgAGATCTagtgtcttcaacggcaccg Ost006R	Reverse oligo for Beta Helix Repeats	ctagcGGATCCtgtacgcatgacaaatgctgac Ost007F	Forward construction of Magnetic Bead BP basic part	ccaaaGAATTCatgAGATCTCACCACAAATACTGGCACCG Ost008R	Reverse construction of Magnetic Bead BP basic part	ctagcGGATCCACGGTGCCAGTATTTGTGGTG Oso001	Cloning of Ag43, short	ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg Oso002 	Cloning of Ag43	GCttaggatcctcagaaggtcacattcagtg Oso003 	Cloning of PL2_Passenger_AT to Autotransporter Ag43	ccataGAATTCatgAGATCTacgctgacgctgaacgacag Ojb001F	Forward construction of CPG_L2 N term part	ctctgGAATTCatgAGATCTgaggaaaggaacgactgg Ojb001R	Reverse construction of CPG_L2 N term part	catgtGGATCCttacgcgctataatctaccggcc Ojb002F	Forward construction of CPG_L2 C term part	ctctgGAATTCatgAGATCTggtaaacgtggaacgtggtt Ojb003F	Forward removal of XhoI site in CPG_L2	actattatctTgagcgcggc Ojb003R	Reverse removal of XhoI site in CPG_L2	gccgcgctcAagataatagt Ojb004F	Forward SOEing oligo for CPG_L2	GGATCTAAGTCTCGTCGCgaggaaaggaacgactggca Ojb004R	Reverse SOEing oligo for CPG_L2	GCGACGAGACTTAGATCCgaacgagtaatttacgccga Ojb005F	Forward construction of CPG_L6 N term part	ctctgGAATTCatgAGATCTgaggaaaggaacgactgg Ojb005R	Reverse construction of CPG_L6 N term part	catgtGGATCCttactgccagtcccagttactcc Ojb006F	Forward construction of CPG_L2 C term part	ctctgGAATTCatgAGATCTgatgatattgaacgtgaagg Ojb002R	Reverse construction of CPG_L2 C term part	catgtGGATCCgaacgagtaatttacgccga Bhs001F	Forward EcoRI for a~eaeA_Display>	cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG Bhs002F	Removing the EcoRI site from eaeA_Display	GTTAATCAGAACTCATTTGCAAATGG Bhs002R	Removing the EcoRI site from eaeA_Display	CCATTTGCAAATGAGTTCTGATTAAC Bhs003R	Reverse BamHI for a~eaeA_Display>	GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC Osn0001-F	Forward Bglbricking of espP of Escherichia coli O157:H7 str. Sakai	ccagtGAATTCatgAGATCTgccccgtcagcatctgccac Osn0002-R	Reverse Bglbricking of espP of Escherichia coli O157:H7 str. Sakai	gcagtGGATCCtcagaacgagtaacggaaattag Odd001F	Forward EcoRI oligo for Tsh Autotransporter	ccataGAATTCatgAGATCTacacttttacctgtatacg Odd002R	Reverse BamHI oligo for Tsh Autotransporter	ctgatGGATCCtcagaatgaataacgaatattagcg Odd003F	Forward oligo for Ice Nucleation Protein	ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg Odmd001F	Forward Oligo for Invasin (refactored, long)	ccataGAATTCatgAGATCTGCTGGGGCTTCAGAAAAATATGATGC Odmd002R	Reverse Oligo for Invasin (refactored, long)	ccaaaGGATCCactggataccagcgatctgccg Ocr0001-F	forward oligo for biobricking of {a~Inv-short>}	ccaaaGAATTCatgAGATCTAATTATGATGGTTTTCCAGC Ocr0001-R	reverse oligo for biobricking of {a~Inv-short>}	ccaaaGGATCCactggataccagcgatctgccgc Odmd003R	Reverse Oligo for Chal Lectin w/o Stop Codon 	ccaaaGGATCCctcgctgtcgtcctctcgta Ons003F	Forward construction of Poly_bind basic part	ccataGAATTCatgAGATCTttcaaattctggctatacgaacatg Ons004R	Reverse construction of Poly_bind basic part	ctgatGGATCCcccccgtattacatgttcgtatagccagaat Ons001F	Forward oligo for TraT	ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc Ons002R	Reverse oligo for TraT	ctgatGGATCCgagaatatttgcgattgatttgg Odvs003	Gene Synthesis of CBK basic part	CCATAGAATTCATGAGATCTGGTTCTGGTCCGACCCAGTCTCACTACGGTCAGTGCG Odvs004	Gene Synthesis of CBK basic part	CGCAAACGGTCGGACCAGAGTAACCGATACCACCGCACTGACCGTAGTGAGACTG Odvs005	Gene Synthesis of CBK basic part	CTCTGGTCCGACCGTTTGCGCTTCTGGTACCACCTGCCAGGTTCTGAACCCGTACTAC Odvs006	Gene Synthesis of CBK basic part	GCTTAGGATCCACCAGAACCCAGGCACTGAGAGTAGTACGGGTTCAGAACCTGGC OWRL-025-F	Forward construction of Aluminum Binding Peptide basic part	GCGTAgaattcATGagatctGTGCCGAGCAGCGGCCCGCAGGATACC OWRL-026-R	Reverse construction of Aluminum Binding Peptide	TCATCggatccGGTGGTGCGGGTATCCTGCGGGCCGCTGC Oph022F 	Foward oligo for cloning of {a~TraA>}	ccaaaGAATTCatgAGATCTacttatgaatgctgttttaagtgttcaggg Oph023R	Reverse oligo for removal of stop codon {a~TraA>}	gcaaaGGATCCgaggccaacgacggccatac js001F 	Forward biobricking of upaG (long) 	CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg js002R 	Reverse biobricking of upaG (long) 	CTGATggatccTTACCACtgaataccggcac Oeh001F	Forward EcoRI/BglII oligo for 	gtcagGAATTCatgAGATCTaaccacagtgatctaattgc Oeh001R 	Reverse BamHI oligo for 	gtcagGGATCCactgtcctgcccggccgttg Odvs002F	Forward construction of AG4 basic part	ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC Odvs002R	Reverse construction of AG4 basic part	ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG OWRL-023-F	Forward biobricking of Intimin EaeA	GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG Odvs0007F	Forward EcoRI/BglII for Bglbrick of ECOHLY	ccataGAATTCATGAGATCTtattccgtggaagaacttattgggacc Odvs0007R	Reverse BamHI for Bglbrick of ECOHLY	ctgatGGATCCataaatatcattaccatatccacc Odvs0008rF	Removing the BglII site in ECOHLY (Forward)	ggcagtgagggagcagaCctgcttgatggcgg Odvs0008rR	Removing the BglII site in ECOHLY (Reverse)	ccgccatcaagcagGtctgctccctcactgcc Omv044F	Forward Bglbricking of {}	ccagtGAATTCatgAGATCTgcaagtatttcatcgctggg Omv044R	Reverse Bglbricking of {}	gcagtGGATCCttaaaaactttgtagcgcatcatcaccgc Omv045F	Forward Removing BamHI site in {}	cgttaagcggCatccgtgatgcc Omv045R	Reverse Removing BamHI site in {}	ggcatcacggatGccgcttaacg Omv046F	Forward Bglbricking of {}	ccagtGAATTCatgAGATCTgttgccgcccagaatgcg Omv046R	Reverse Bglbricking of {}	gcagtGGATCCcttggaattactgttgttttcg Ovu015	Construction of cpx N term part	ctagaGAATTCatgAGATCTGGTCAGTCTggtgactacaacaaaaaccag Ovu016	Construction of cpx N term part	gacaaGGATCCgaagcggtaaccaacagaggcaatccaggtgcctac Ovu017	Construction of cpx C term part	ctagaGAATTCatgAGATCTgcgacttctactgtaactgg Ovu018	Construction of cpx C term part	gacaaGGATCCttaagagcttgcagtacggcttttctcgg Ovu019	SOEing of eCPX	gtcgcTTTAGACTGTTTAGATCCgaagcggtaaccaacag Ovu020	SOEing of eCPX	GGATCTAAACAGTCTAAAgcgacttctactgtaactgg Osc0030F	Forward Biobricking of ehaB	ggcctGAATTCattAGATCTggcttaatcgcaatgaactct Osc0031R	Reverse Biobricking of ehaB	gtaccGGATCCttaccaggtatatttaacac Omv047F	Forward construction of { } basic part	ccataGAATTCatgAGATCTtacttcgatgtctggggcgcagggaccacggtcaccgtctcctcaggcggag Omv047R	Reverse construction of { } basic part	ctgatGGATCCtcctcctccagaaccacctccacctgaaccgcctccgcctgaggagacggtgac Omv048F	Forward construction of { } basic part	ccataGAATTCatgAGATCTtggacgttcggtggaggcaccaagctggaaatcaaacgtgcggccgctc Omv048R	Reverse construction of { } basic part	ctgatGGATCCacggccttcaatgccgtgatggtgatggtggtgagcggccgcacgtttgatttc Ojw001	Forward construction of Z34C basic part	CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG Ojw002	Reverse construction of Z34C basic part	CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG Oph024F 	Foward oligo for cloning of {}	ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg Oph025R	Reverse oligo for cloning of {}	ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc Osc0028F	Forward construction of Leucine zipper EILD	TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG Osc0029R	Reverse construction of Leucine zipper EILD	CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG 