User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/29

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01/29/10

 * &#x2713; SOEing PCR: repeat megaprimer PCR from 1/28
 * &#x2713; Cultures: KAH139/V0201 (4)

> Alternative Step 2 --> Repeat "megaprimer" PCR from 1/28; 4 reactions

--> BioRad Block A
 * 95°C/ 3 min.
 * [95°C/ 30 sec., 57°C/ 30 sec., 72°C/ 30 sec.] x35
 * 72°C/ 3 min.
 * 4°C/ ∞

--> PCR didn't work. Try site-directed mutagenesis tomorrow.


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