User:Steven J. Koch/Notebook/Kochlab/2008/07/16/Lambda DNA visualization


 * Aliquoted YOYO1 into 20 ul aliquots ... now stored at -20C


 * Making 10x YOYO1 stain:
 * 999 ul TE buffer (from Sandia)
 * 1 ul YOYO1 stock
 * covered tube partially with masking tape, is in drawer


 * Made working DNA solution
 * 88 ul TE buffer
 * 1 ul Taq
 * 1 ul lambda DNA stock (vortexed and heated by hand...was still very viscous)
 * 10 ul 10x YOYO solution (above)
 * Did not see any stretched out DNA, but PLENTY of DNA balled up in solution. Fading bad, but not too bad.


 * Adding 10 ul taq to the remaining about 75 ul of the above working solution.
 * The sample leaked (or probably I flowed it around the edge), so we made a new sample, and watched as we flowed in the new higher taq
 * Did not look any different than with less taq. Not proving anything, but best guess is that taq is not working like the RT was.