Notebook:Tk/2008/04/14

Transformations

 * Some small colonies of a few of the older plates
 * likely junk

PCR of transposons

 * 20 ul reactions
 * 0.6 ul ME0 primer
 * cycle 95/5 min, (95/20s, 55/20s, 72/3.0 min) x 32
 * wells:
 * 83 ng/ul cut PCR'd transposon
 * transposome
 * cells from culture B of initial transformations (streaked plate for growth test)

Setup of cultures for tomorrow

 * 50 ml cultures infected with
 * 5 ml
 * 0.5 ml
 * 50 ul
 * 5 ul