User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/07

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To do

 * Desiccate rotifers for
 * re-extract failed rotifers

Rotifer dessication

 * prepare rotifers
 * take three vials of each species to be tested
 * one as a test rehydration, one to take to Japan, one to keep in the Mark Welch lab
 * Add small pieces of filter paper to the rotifer culture, and remove most water with a pipette, leaving .3-.5 ml
 * note number of rotifers
 * prepare humidity chamber
 * The dry salt is spread about 3 mm deep in a petri dish that occupies most of the bottom of a small airtight bottle.
 * Water is added to moisten the salt -- do not add more water than is needed to make the salt look damp.
 * keep each salt for about 4 hours, then replace with the one giving a lower relative humidity.
 * keep under Potassium carbonate until dry (also for long-term storage @ rt)
 * once dry, put both vials at -80C


 * Relative humidity table


 * We can also use Sodium bromide (RH 57.6%) as an intermediate, if necessary