Sauer:Protocols

Please link protocols to the general Protocols page if appropriate.

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Protein Expression/Purification
Sauer:ClpX purification

Sauer:His6 ClpX purification

Sauer:ClpP purification

Sauer:ClpA purification

Sauer:SspB purification

Sauer:Expression/purification of 35S-Met proteins

Sauer:Purification of His6-tagged proteins

Sauer:Lysing E. coli with Lysozymes

Sauer:GFP-H6-ssrA purification

Sauer:GST-sFtsH purification

Sauer:FtsH purification

In vitro enzymatic assays
Sauer:In vitro degradation of GFP-ssrA by ClpXP

Sauer:In vitro degradation of GFP-ssrA by ClpAP

Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED!

Sauer:ATPase assays

Sauer:In vitro peptide degradation by ClpXP

Proteins: in vitro
Sauer:Silver staining

Sauer:bis-Tris SDS-PAGE, the very best

Nucleic acids: in vitro
Sauer:In vitro transcription with T7 RNA polymerase

Sauer:Phenol/chloroform extraction

Quantitating nucleic acids

Sauer:Site-directed mutagenesis

Sauer:RNA Purification from E. coli

Sauer:Stitching Genes by PCR


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Analysis of proteins or RNA in vivo
Sauer:Western blot

Sauer:Quantitative Western blot

Sauer:Northern blot PROTOCOL NEEDED!

Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED!

Sauer:beta-Galactosidase Assay (A better Miller)

More in vivo goodies
Sauer:Competent cell preparation PROTOCOL NEEDED!

Sauer:P1vir phage transduction

Sauer:Plasmid-born λRed recombineering using dsDNA

Other good stuff
Generation of genomic libraries

Sauer:HPLC protocol

Sauer:FPLC protocol


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