IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/17/Min Lin

Transformation( ligation product into Top10) Miniprep SupD plasmid. Use E.coli with T7ptag (From Voigt Lab) to make competent cell. Pour LB plates with double antibiotics (Kan and Cm). Transform the competent with SupD plasmid.