Koch Lab:Protocols/GPEM

GPEM is PEM + 1 mM GTP. PEM is BRB80 and also referred to as "general tubulin buffer."

As of June, 2009, we are making GPEM as follows:


 * 98 parts PEM
 * 2 parts 50 mM GTP

For example, on 6/15/2009, Linh is making 300 microliters of GPEM by adding:
 * 294 microliters PEM (from cytoskeleton)
 * 6 microliters of GTP