BCH092406-Construction of pJ23006-J23085

[tRNA key precursor J23061] + [B0015] PCR ca998/G00101 on pSB1AK3-B0015 template (XbaI/PstI, neb3, 226bp) Paste into pJ23006-J23061 (SpeI/PstI, neb2, large) Product is pJ23006-J23085