IGEM:Peking University/2008/Experiment/PKU 0807022 G1 1

PKU_080722_G1_1

 * Positive transformation-pGREG504(new)/DH5α/JM109


 * Picking up clonies


 * Gel running and testing-hypothesis-promoter's ligation-(lac1/lacS/tet)(SB/SX)AID(BX)


 * result:no band and snear


 * Sequencing-ligation product(yesterday)


 * Ligation test-pGREG504-promoter-old/new ligation buffer


 * Enzyme cutting and testing-pGREG504-single(sacI/XhoI)-double(sacI&XhoI)