User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/05

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Start stimulation ELISA & IF, splitting cells
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Cells for ELISA were stimulated with CSE and treated with db-cAMP or mPKI (all had at least DMSO) (15.30)
 * Cells for IF were stimulated with CSE with and without DMSO or Ht31 (15.55)
 * NOTE: Ht31 was diluted in S0 OR 15% CSE!! So no incubation with Ht31 prior to adding CSE!
 * New cells were split to P24 (4x 100mm dishes)


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