Phusion

Description
Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Statistics about Phusion™ High-Fidelity DNA Polymerase

 * 10x processivity compared to Taq
 * 50x fidelity compared to Taq
 * Creates blunt end
 * Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
 * Non-displacing

Quick reference reaction mix
See the manual for details and special usage conditions.

A 2x supermix is now available containing either HF buffer or GC buffer, dNTPs, and Phusion polymerase. See references section below for links.

Thermocycling conditions

 * 1) 15-30 s/kb extension time.
 * 2) 98C for denaturation.
 * 3) Anneal at 3C above the Tm.
 * 4) *Note: Tm should be calculated with nearest neighbor method see: Finnzyme Tm calculator
 * 5) Extend at 72C.