Escherichia coli RNA polymerase

Epicentre is the only commercial source for Escherichia coli RNA polymerase.

Materials

 * E. coli RNA Polymerase Core Enzyme and Sigma-Saturated Holoenzyme from Epicentre

For core enzyme
from Epicentre

For sigma saturated holoenzyme
from Epicentre

Functional test
50 &mu;L reaction
 * 1X E. coli RNA polymerase transcription buffer
 * 0.04 M Tris-HCl (pH 7.5)
 * 0.15 M KCl
 * 10 mM MgCl2
 * 0.01% Triton® X-100
 * 2mM DTT
 * 0.25mM NTPs
 * 1&mu;g T7 D111 DNA as template

Assay test
50 &mu;L reaction
 * 1X E. coli RNA polymerase transcription buffer
 * 0.04 M Tris-HCl (pH 7.5)
 * 0.15 M KCl
 * 10 mM MgCl2
 * 0.01% Triton® X-100
 * 10 mM DTT
 * 0.5 mM NTPs
 * 1&mu;g T7 D111 DNA as template