Endy:Screening plasmid/v1.0

Design



 * Endy:Screening plasmid/v1.0/Update

Controls

 * I13522 - constitutive GFP
 * I13521 - constitutive RFP
 * I13540 - inducible GFP
 * I13520 - inducible RFP
 * V1023 - negative (CW2553/pJAt8)

Characterization of Empty Screening Plasmid 1.0
To ensure our screening system was operating as expected we measured the expression levels of GFP and mRFP1 when the plasmid did not contain a part (e.g. input equals output).
 * Endy:Screening plasmid/v1.0/Hairpin - characterize effectiveness of the hairpin to stabilize the mRFP1 transcript
 * Endy:Screening plasmid/v1.0/RNAseE sites - characterize effectiveness of the RNAseE sites
 * Endy:Screening plasmid/v1.0/Growth rates - characterization of growth rates under different induction levels / conditions
 * Endy:Screening plasmid/v1.0/Steady state - evaluate expression levels at various time points following induction.
 * Endy:Screening plasmid/v1.0/Characterization protocol

Terminator Characterization via Screening Plasmid 1.0

 * Protocols
 * Analysis

Inverter Characterization via Screening Plasmid 1.0

 * Protocols
 * Inverters
 * Analysis

Inverter library screening via Screening Plasmid 1.0
To be added.


 * Endy:Screening plasmid/v1.0/Glycerol box 4