Muratore:Protocols/PCR/Basic


 * Add each from left to right. Mix and spin before adding Pfu Turbo. Gentle mixing and spin after Pfu Turbo. Don't mix in wax.
 * 1) 5' @ 95°C
 * 2) 30" @ 95°C
 * 3) 2' @ Tm-5°C
 * 4) 2' @ 78°C
 * 5) repeat steps 2-4 x 29 more times
 * 6) 5' @ 78°C