Neutral red

Overview
Neutral Red for gHV68 (DW)

Materials

 * Neutral Red
 * Whatman filter paper
 * Bottles

Procedure
Stock Solution:


 * 1) Add 25g neutral red to 2 liters water.
 * 2) Incubate o/n at 56C protected from light.
 * 3) Filter through Whatman filter paper.
 * 4) Aliquot into small bottles and wrap in tin foil.
 * 5) Autoclave.
 * 6) Store stock at RT.

Working solution:


 * 1) Dilute 3 ml stock solution per 100 ml PBS.
 * 2) Filter.
 * 3) Apply to methylcellulose.

Contact

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