Crisanti:Materials and Orderforms

[[media:Order Form.doc|Click here for plain orderform!]]

PCR
[[media:Crisanti-Taq.doc|Click here to complete the order form]] [[media:Crisanti-BDAdv.doc|Click here to complete the order form]] [[media:crisanti-phusion.doc|Click here to complete the order form]] [[media:Crisantiphusionhs.doc|Click here to complete the order form]] [[media:primers.doc|Click here to complete the order form]] [[media:FastSYBRgreen.doc|Click here to complete order form]] [[media:Crisanti_PCR_strip_tubes_sep_cap.doc|Click here to complete order form]] [[media:Crisanti_ExoSAP.doc|Click here to complete order form]]
 * Taq DNA Polymerase
 * BD Advantage 2 PCR Kit
 * Phusion™ High-Fidelity DNA Polymerase
 * HS Phusion™ High-Fidelity DNA Polymerase
 * MWG Primers
 * Fast SYBR® Green Master Mix
 * PCR strip tube separate cap
 * ExoSAP-IT (USB)

Competent Cells
[[media:CrisantiTop10.doc|Click here to complete the order form]] [[media:Subcloning_efficiency.doc|Click here to complete the order form]]
 * One Shot® TOP10 40x50ul
 * Subcloning Efficiency Commpetent Cells

Enzymes

 * Restriction Enzymes

[[media:AntaPhosphatase.doc|Click here to complete the order form]] [[media:Crisantinebligase.doc|Click here to complete the order form]] [[media:Crisantinebventpol.doc|Click here to complete the order form]] [[media:Crisantinebventexopol.doc|Click here to complete the order form]] [[media:crisantiascifermentas.doc|Click here to complete the order form]] [[media:crisantiasciNEB.doc|Click here to complete the order form]] [[media:crisantiDpn1NEB.doc|Click here to complete the order form]]
 * NEBs Antarctic Phosphatase
 * NEB T4 DNA Ligase
 * NEB VentR DNA Polymerase
 * NEB VentR (exo-) DNA Polymerase
 * Fermentas AscI FAST digest
 * NEB AscI digest
 * NEB Dpn1

Cloning Kits
[[media:PGEM_orderform.doc|Click here to complete the order form]] [[media:Crisanti-Pet101.doc|Click here to complete the order form]] [[media:CrisantiZeroblunt.doc|Click here to complete the order form]] [[media:Crisantitakara.doc|Click here to complete the order form]]
 * pGEM®-T Easy Vector System I
 * pET101 TOPO Expression Kit
 * Zero Blunt Topo PCR cloning Kit
 * Takara DNA Ligation Kit 2.1

Kits (Qiagen, Promega)
[[media:Crisanti-QuiagMAXI.doc|Click here to complete the order form]] [[media:Crisanti-Quiagelextr.doc|Click here to complete the order form]] [[media:highspeedMAXI.doc|Click here to complete the order form]] [[media:Crisanti-QuiagEndoFreeMAXI.doc|Click here to complete the order form]] [[media:Crisanti-QuiaPCRpur.doc|Click here to complete the order form (50)]][[media:Crisanti-QuiaPCRpur250.doc| (250)]] [[media:CrisantiWizard.doc|Click here to complete the order form]] [[media:Crisanti-QIAprepMINI.doc|Click here to complete order form]]
 * QIAgen Plasmid Maxi Kit (25)
 * QIAquick gel extraction kit
 * Hispeed Maxi
 * EndoFreeHispeed Maxi
 * PCR purification Kit
 * Wizard® Genomic DNA Purification Kit
 * QIAprep Spin Miniprep Kit (250)

RNA
[[media:Crisantirnasefreestuff.doc|Click here to complete the order form]] [[media:Crisanti megascript.doc|Click here to complete the order form]] [[media:Crisanti megaclear.doc|Click here to complete the order form]] [[media:Crisantiambiontrna.doc|Click here to complete the order form]] [[media:Crisantisuperrnasin.doc|Click here to complete the order form]] [[media:Crisantinorthernmax.doc|Click here to complete the order form]] [[media:Crisantibiotin.doc|Click here to complete the order form]] [[Media:CrisantiRACE.doc|Click here to complete the order form]] [[Media:CrisantiNORTH2.doc|Click here to complete the order form]] [[Media:CrisantiMMM.doc|Click here to complete the order form]] [[Media:CrisantiFrancescaOrder1.doc|Click here to complete the order form]]
 * RNA free tips (1000,200,10ul) and tubes
 * MEGAscript® High Yield Transcription Kit 25rxn
 * MEGAclear® Kit 20rxn
 * Yeast tRNA (10 mg/ml)
 * SUPERase•In™ (20 U/µl) 2500u RNAse Inhibitor
 * NorthernMax® Formaldehyde Load Dye 6x1ml
 * Ambion 10 mM Bio-16-UTP
 * Ambion RACE Kit
 * Ambion Northenmax2 kit
 * Ambion T7 MessageMachine
 * Francescas Ordeform

Electrophoresis
[[media:Hyperladder form.doc|Click here to complete the order form]]
 * Bioline Hyperladder 1 (200-10 000 bp)

Cell Culture
[[media:FBS.doc|Click here to complete the order form]] [[media:Schneiders.doc|Click here to complete the order form]] [[media:Effectene.doc|Click here to complete the order form]] [[media:Nunc175.doc|Click here to complete the order form]] [[media:Crisantipenstrap.doc|Click here to complete the order form]]
 * Invitrogen's FBS
 * Invitrogen's Scheider's Drosophila Medium
 * Effectene Qiagen
 * Easy Flasks
 * Penicillin-Streptomycin Solution, liquid 100ml

Protein Stuff & Antibodies
[[media:OrderHa-Antibody.doc|Click here to complete the order form]] [[media:CrisantiLeupeptinantipain.doc|Click here to complete the order form]]
 * Anti-HA Tag mouse Monoclonal
 * Leupeptin & Antipain Protease Inhibitors

Radioactive Isotopes
[[media:32POrder_Form.doc|Click here to complete the order form]] [[media:UTP_p32t.doc|Click here to complete the order form]]
 * α-P32 dATP
 * α-P32 UTP

Chemicals
[[media:CrisantiGlucose.doc|Click here to complete the order form]]
 * Glucose (for teh Mossies)

Injections
[[media:Femtotips.doc|Click here to complete the order form]] [[media:Microloader.doc|Click here to complete the order form]] [[media:Micoscope_Slides.doc‎|Click here to complete the order form]] [[media:8_well_slides.doc‎|Click here to complete the order form]]
 * Femtotip (from VWR)
 * Microloader (from VWR))
 * Microscope Slides (from VWR)
 * 8 well Microscope Slides (from MP Biomedicals)

Microarray Group stuff
[[Media:CrisantiTyramide.doc|Click here to complete the order form]] [[Media:Crisantitips.doc|Click here to complete the order form]] [[Media:Crisantisilicagel.doc|Click here to complete the order form]] [[Media:Crisantimicroplates.doc|Click here to complete the order form]] [[Media:CrisantiFischerwater.doc|Click here to complete the order form]] [[Media:CrisantiFedex.doc|Click here to complete the order form]] [[Media:CrisantiCelAs.doc|Click here to complete the order form]] [[Media:Crisantisyringefilter.doc|Click here to complete the order form]] [[Media:CrisantiGeneFrames.doc|Click here to complete the order form]] [[Media:CrisantiMediaHybridoma.doc|Click here to complete the order form]] [[Media:CrisantiFlasksHybridoma.doc|Click here to complete the order form]] [[Media:CrisantiProteinAColumnHybridoma.doc|Click here to complete the order form]] [[Media:CrisantiBSA.doc|Click here to complete the order form]] [[Media:CrisantiAlexaAntibodies.doc|Click here to complete the order form]]
 * Tyramide
 * Tips
 * Silica Gel
 * Microplates
 * Fischer Water
 * Cel Associates Fedex
 * Cel Associates
 * Syringe filter
 * Gene frames
 * Media Hybridoma
 * Flasks Hybridoma
 * ProteinAcolumn Hybridoma
 * BSA
 * Alexa Antibodies

Blotting
[[media:Hyperfilm_ECL.doc|Click here to complete the order form]] [[media:Hybond_ECL.doc|Click here to complete the order form]] [[media:Hybond_N.doc|Click here to complete the order form]]
 * Hyperfilm ECL
 * Hybond ECL Nitrocellulose Membrane
 * Hybond N+ Roll 1m Nitrocellulose Membrane

Safety and Waste
[[media:Crisanti_sharps.doc|Click here to complete the order form]]
 * Sharps container, for non-infectious sharps and needles

Other Stuff
[[media:Crisantig50.doc|Click here to complete the order form]] [[media:CrisantiCellstrainers.doc|Click here to complete the order form]] [[media:CrisantiGibcowater.doc|Click here to complete the order form]] [[media:CrisantiColumns.doc|Click here to complete the order form]] [[media:Crisantivwrnalgene.doc|Click here to complete the order form]] [[media:CrisantiBioradgelstuff.doc|Click here to complete the order form]] [[media:Crisantidialysis.doc|Click here to complete the order form]] [[media:Crisantisilversnap.doc|Click here to complete the order form]] [[media:CrisantiDynabeads.doc|Click here to complete the order form]] [[media:Crisantistreptavidin.doc|Click here to complete the order form]] [[media:Crisanticoll.doc|Click here to complete the order form]] [[Media:Crisantilamps.doc|Click here to complete the order form]] [[media:Pinzers.doc|Click here to complete the order form]] [[media:Snap-cap.doc|Click here to complete the order form]] [[Media:Crisanti50ml-15ml tubes.doc|Click here to complete the order form]] [[Media:CrisantiEppendorf tubes.doc|Click here to complete the order form]] [[Media:CrisantiBlueRollsandKimwipe.doc|Click here to complete the order form]] [[Media:Crisanti_5xloadingbuffer.doc|Click here to complete the order form]]
 * illustra ProbeQuant™ G-50 Micro Columns
 * BD Falcon™ Cell Strainers, yellow, 100 μm
 * Ultrapure DNAse/RNAse-Free distilled water 500ml
 * Chromatography Columns, empty 50
 * Nalgene Filter units + Bottles, sterile 250ml
 * Biorad gel equipment
 * Slide-A-Lyzer® Dialysis Cassettes 10/pkg 7000MWCO
 * SilverSNAP Staining Kit for Mass Spectrometry
 * Dynabeads® M-280 Streptavidin 10 mL
 * ImmunoPure Immobilized Streptavidin, 	5 ml
 * Colloidal Blue Staining Kit, G250 Coomassie for Masspec
 * Microscope Lamps UV and TM
 * Pinzers aka: tweezers
 * Snap-Cap 14ml Tubes for mini-cultures
 * 50ml-15ml tubes
 * Eppendorf tubes
 * Blue rolls and kimwipe
 * Crystal 5x DNA Loading Buffer Blue, 2x1ml (Bioline)