Klapperich Lab:Notebook/Lab Meeting Notes/2009/06/23

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

23 June 2009 Lab Meeting
Missing:Alex, Jaephil, Ajani, † Announcements † Flu R01
 * Attending: Hussam, Paolo, Aaron, MinCheol, SOnali, QQ, Jane, Cathie, Brendan, Jessie.
 * Hussam will present on MS and FITR data.
 * I need a poster on HDA for the conf. next week. I leave Sat. PM. May I have a soft copy of the old poster and all of the new data/pictures?
 * MicroTAS (4) Papers due June 30th.
 * Integration: Spec? On chip? Jane will look into it.
 * QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR. Cutter plotter? Faunhofer Chip serpentine channel.
 * RNA extraction troubleshooting.
 * Try clean fabrication of chips and monoliths.
 * Try RNAseH wash through.
 * Absolute Quantification assays:
 * A primers look good at this point. Van Elden 2001.
 * Jessie should learn SEM.

† Virus concentration (upstream from the assay.) † Coulter Flu Fraunhofer Project † SEPSIS Project †RNA project † COBRA paper 1: Evap with Sol Gel substrate. paper 2: Covap with PMA, Rhodamine, PS beads?...virus...? - Rhodamine experiments done - Signals are similar between with and without GNP - Rhodamine binds poorly to gold - try GNP-pMA next. R6G-silver NP. - Need to redefine hypothesis
 * QQ - HM - JZ - Chip Integration.
 * How to read? Color, sensitivity? Alignment. Check with JD.
 * Fraun folks coming to get trained next week. RNA isolation. Safety issues. English.
 * IBC for Fraun flu. waiting?
 * Brendan can come to Fraun Flu meetings if he wants....as primer design goes.
 * Ajani should come to Fraun Flu meetings to learn how to make straws. Alex will train him.
 * Proofs in.
 * Call Natalia.
 * Update on concentration of live bugs?
 * Evap system (JD and JZ)
 * Let's get this to a point where we can publish and move on

† Biointerfaces group † CIMIT- Colson Grant † PCR it has been uploaded in the share driver. done done the repeatebility has been comfirmed. † RCA/HDA
 * fabricated SU-8 mold with Herringbone grooves.
 * Brett took a photo of the device for the paper, but will repeat
 * Jason coated thin gold on the surface of the PDMS mold, and will take SEM images for the sieves. --->SEM down
 * draft paper2 for the submission to "Biophysical Journal" has been started.
 * Will repeat trapping experiments this Friday to obtain good quality images for the Fig. 2 in the paper.
 * IRB submitted?
 * QQ: PCR 2 paper draft.
 * CMK: PCR 1 draft
 * DONE with PEG attempts.
 * PEG-coating protocol developed - TROUBLESHOOTING.
 * on-chip experiment with blank chip without PEG,BSA. It works with expected lower efficiency
 * on-chip experiment with PEG-coated chip without PEG,BSA in reagent. It does not work, or the product is under the detection limitation of bioanalyzer.
 * QQ : write protocol for PEG graft.
 * QQ: Try changing heater and thermocouple.
 * QQ: Try running PCR after plasma treatment and water wash only.
 * QQ and MM: look back at flu PCR. Recall the repeatability. Decide course of action with Sonali. See what you can do to do "real" samples soon. One pot? Two steps necessary instead?
 * Getting solution back out is still an issue.
 * Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime)
 * Evaporation problems near edges. Maybe design change?
 * Teflon issue with the enzyme? Check into it.

† Silica Optimization (Lambda):
 * Do absolute quant assay. Mass Spec data suggests junk is polymer. Pursue GPC later.
 * Get lower Bioan. Kit.


 * }