Klapperich Lab:Notebook/Lab Meeting Notes/2008/11/18

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18 November 2008 Lab Meeting Agenda
† Alex's research report today.

† Announcements † Flu R01 † SEPSIS Project - CIMIT Bird Flu †RNA project † COBRA † Valve Array/Valve building (FJ) † Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR '''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ''' † RCA † Senior project † F31: Cochlea † Silica Optimization (Lambda):
 * Room 720 move out.
 * First samples taken starting next week, 11/19.
 * Sample training 9:30am Wed.
 * Plaque Assay - first samples today. Ref stock 2x10^4 pfu/ml
 * Mark trained for real to make SU molds. Trained to make the monoliths.
 * Hussam: Flu fixture will be ready 11-19-08 (tomorrow)- 6 channel fixture (2).
 * Cathie STILL working on paper.
 * Jeff Braman will visit December 12th
 * Do cDNA prep. Spec, gels, send to Jeff B. Shipping?
 * Mark- Control circuit?
 * Bacteria Experiment - Changing channel size.
 * Substrates -SEM down.
 * Valves backorderd until 24th.
 * Mask made. Chrome mask.
 * E. Coli experiments with fluorescence?
 * Aim for fluor data for 6th.
 * Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
 * STAR-CD Renewal - January ($2K)
 * Cathie's flu assay has nonspecific products, too.
 * Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?
 * Look into Melting temp analysis on the 7300 - Done. what is contaminated?
 * Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit.
 * Design new lambda phage assay with longer product.
 * What do we need to finish the paper?
 * Cathie submitted One pager Whitepaper. Waiting for response.
 * Megan has mask designs. CHECK WITH HER.
 * Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume. 100 microns.
 * Mark will help/learn SU8 step.
 * Teddy doing off chip controls work.
 * Submit paper.
 * UPDATE 11-18-08
 * De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. (today)
 * Ongoing. More concentrations. saved samples in -20 until pellet paint figured out
 * Repeating experiments this week with activated MMA and collecting more samples
 * Plates used for Pico Green are not sterile ?? NOT LIKELY.
 * Pico Green assay figured out and standards working.
 * Pellet paint: Even (-) ctrl form a pellet, after 100% ethanol wash pellet is detached.
 * try std. etoh protocol.


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