User:Karmella Haynes/Notebook/miR Trigger/2009/12/27

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12/27/09

 * &#x2713; Minipreps: KAH93, 94, 95/MV2 (from re-transformation colonies)
 * &#x2713; Transfections: KAH120-125/MV2 (12-well format, Fugene)

Minipreps > Check with E/P digests

Fugene transfection > KAH120-125/MV2 + U2OS plain (12-well format, Fugene)

> 3.6 μL Fugene + 46.4 μL Opti-MEM --> R.T/ 5 min. > Add DNA --> R.T./ 20 min. > Add 50 μL complexes to each well (2 ml med. each); Grow cells at 37&deg;C overnight

Day 2 > Check RFP/CFP expression --> Experiment looks interesting so far. Document images and repeat again with full set of 9 pairs when KAH117-119 are finished


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