User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/11

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] AKAP250 (scbt) immunoblot cAMP precipitation
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Put secondary antibody on blot of cAMP precipitation (after 3x 10 min. wash with blocking buffer)
 * anti-goat 1:5000 in blocking buffer
 * Put primary antibody on blot of AKAP250 co-IP
 * Should have cut both membranes, but will now do RII immunoblot after this one

Results

 * cAMP precipitation immunoblot for AKAP250 using the Santa Cruz C-16 antibody

Discussion

 * maybe too much secondary antibody? a bit high background So I am not sure if it is not there (or is)
 * should try the new antibodies


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