IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/30

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Entry title

 * Order: S+I(1,2); S+I(3,4)
 * Gel run with entire template:
 * NO PCR product found
 * PCR of S+I7 with primers 1 and 2
 * PCR program:
 * 1) 98°C for 2min;
 * 2) 98°C for 30s;
 * 3) 64.4°C for 30s;
 * 4) 72°C for 45s;
 * go to 2 for 30 times
 * 5) 72°C for 30min;
 * 6) 4°C hold
 * inoculated DH5alpha in SOB for competant cell preparation.


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