Drummond:Protocols

Local Protocols
Here are some protocols we use in the lab.


 * Resolving and detecting ubiquitin
 * Using paromomycin to alter translation in yeast
 * Cell preparation and protocol to view RFP/TFP fluorescence
 * Using the Zeiss LSM 510 confocal microscope
 * Yeast OD and cell viability model
 * Standard conditions for flow cytometer growth rate assay
 * High-efficiency yeast transformation
 * Yeast genomic DNA prep
 * Isolating total, soluble and insoluble protein fractions
 * Making chemically competent E. coli cells
 * Preparing anti-ubiquitin beads
 * Periplasmic protein prep from E. coli

Remote Protocols

 * Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
 * Rather rapid yeast genomic DNA prep (Gottschling Lab)
 * SDS-PAGE (Szostak Lab)

Data Analysis Protocols

 * Akashi's test for translational accuracy selection