User:Tk/Notebook/MF/2008/07/28

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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 * style="background-color: #F2F2F2" align="center"|  |Main Page


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Resequencing of problematic Me. florum regions

 * Primers for PCR and sequencing


 * ttaggttttcacttaccaagaaaagg,F0050, 532951R
 * actacagctatttcgtcagttaaagtaa,F01063, 531938R
 * aacggaattaacgaaaagttagca,R01280, 531731F
 * ggattttcttttagtaatagttgcagc,F02050, 530951R
 * cattaaccataaataccaacattcca,R02227, 530774F
 * taaagatggcaagtagaattaccaaa,F03226, 529775R
 * cctagtcctgaattacatccaaa,R03296, 529705F
 * gagctagtggagtttatagttcagaatc,F04220, 528781R
 * gcacgatataggttttttcacttc,R04281, 528720F
 * cagtctctgaaagagctaaaattga,F05200, 527801R
 * ctttttcaatcgcaatgcttc,R05304, 527697F
 * aggagaagtggaatgagataagga,F06212, 526789R
 * aattcgccatcaaattctga,R06307, 526694F
 * gcatgagatacatcaaaagtaactga,F7152, 525291R
 * gctgaagcatcagaaaacatact,R07282, 525719F
 * ttgactatctcaaagtatagattagtgttttt,F08163, 524838R
 * tatctgataaaatacctaaacctagaattaac,R08268, 524733F
 * atataacccattttgagataaaatgattg,F09100, 523901R
 * acgtaaaaagaaaggaattataaatttga,R09225, 523776F
 * tagtggttgttacattaacaacaaacg,F10092, 522909R
 * cttccaattttgattgcaaga,R10199, 522802F
 * gatgaagcacataaaaatcaagc,F11042, 521929R (several other matches)
 * agttgaatcatcttcagtaacatcaaa,R11146, 521855F
 * tcagctgctaaaggaaatgatg,F12017, 518806R
 * ttaacagttgaaacatcatttccttt,R12141, 518772F
 * gatttagcagatattattaaagctgatga,F13112, 518845R (dozen other matches to 521193, every 261 bp)
 * ccagctgcagttaacacagttt,R13218, 518739F (dozen other matches to 521xxx)
 * ttgttgatggttctaaagtaacttttg,F14100, 518640R (dozen other matches)
 * cagttgaaacatcatttcctttagc,R14225, 518776F (half dozen other matches)
 * ctgatgaaaacataatgtttgaattaa,F15090, 517911R
 * ttacctcttttctaacttcatattgttatt,R15314, 517687F
 * tcgtgtagttagataccaccctaaga,F16076, 516925R
 * cttttgtatatggttgtctgtgacc,R16146, 516855F
 * gagcgtctggtgaatcaatc,R16977, 516024F


 * }