User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/01/22

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis
Today:
 * With gfp-myb, rfp-h2av samples: Heather performed the following yesterday (1/21/10):
 * Incubate with primary antibody in 2% NGS
 * O/N at 4C
 * Anti-sa with 1:100 anti-sa from guinea pigs
 * Anti-bam with 1:5 anti-bam from mouse
 * Totpro with nothing, continued blocking
 * Wash 3 x 10 minutes in 1x PBS-T
 * Incubate with secondary antibody in 2% NGS
 * (1:500 of respective secondaries conjugated with 633 fluorophore)
 * Except totpro, remained in 1x PBS-T
 * 2hr at RT
 * Wash 3 x 15 minutes in 1x PBS-T
 * Keep over the weekend in PBS-T.
 * Will stain totpro sample with totpro on Monday and mount the anti-bam and anti-sa samples.


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