Quint Lab:yeast western blot

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western blot yeast

 * 1) spin down the over night culture 2 min 10000 rpm
 * 2) pour off the SN
 * 3) add 2x Lämmli: 50 – 100 µl → vortex
 * 4) boil for 5 min → chill on ice
 * 5) spin down 30 s top speed→ chill on ice
 * 6) 15 µl on gel
 * 7) 2 ml over night culture (selective medium) @ 30°C

pGBKT7: α-c-myc (1:5000) pGADT7: α-HA (1:5000) pJG4-5/pJCL: α-HA (1:5000) pEG202: α-LexA