Quint Lab:Back Door

{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 790px; color: #000000; background-color: #ffffff;" |width=395px style="padding: 5px; background-color: #ffffff; border: 2px solid #000101;" |
 * -valign="top"

resources

 * primer lists
 * enzyme lists
 * seeds
 * ordering


 * width=395px style="padding: 5px; background-color: #ffffff; border: 2px solid #000101;" |

papers/lab meetings/seminars
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 790px; color: #000000; background-color: #ffffff;"
 * papers of the month
 * lab meeting/journal club
 * ipb progress reports/seminars
 * SFB648 seminars
 * }
 * -valign="top"
 * width=395px rowspan=2 style="padding: 5px; background-color: #ffffff; border: 2px solid #F8B603;" |

online tools
dna/protein analysis:
 * sequence converter
 * dna clean-up
 * protein clean-up
 * reverse complement
 * translate dna
 * predict protein mw
 * protein motif scan
 * restriction analysis
 * genetic code
 * amino acid structures
 * plasmapper

oligos:
 * oligo analysis
 * primer3plus
 * duplex analysis

blasts/alignments:
 * pairwise dna alignment
 * protein alignment
 * blast alignment
 * clustalw - multiple alignments
 * blastn
 * blastp
 * Vicogenta - comparing genomes to arabidopsis

arabidopsis tools:
 * arabidopsis database collection
 * tair
 * nasc
 * 101 ways to grow arabidopsis
 * plants-ubq
 * salk t-dnas
 * genevestigator
 * efp-browser/expression angler/etc.
 * GEB - gene expression browser for arabidopsis
 * tiling array expression atlas
 * arex
 * mapman
 * web mirna designer
 * tile viz
 * tiling array express
 * 1001 genomes
 * POLYMORPH reseq SNPs
 * arabidopsis hormone database
 * JIC microsatellite mapping markers
 * Vicogenta - comparing genomes to arabidopsis
 * AmiGO - Gene Ontology
 * PLAZA
 * dCAPS finder

medicago tools:
 * medicago gene expression atlas
 * medicago database

natural variation/polymorphisms:
 * msqt - multiple snp query tool
 * masc snp database
 * caps finder
 * polymorph - reseq. snps
 * atprimer ecotypes
 * JIC microsatellite mapping markers
 * Marker Tracker
 * Bergelson A.thaliana Resources

calculators:
 * vector:insert ratios
 * molar conversions - moles ↔ weight
 * concentration conversions - molar units ↔ weight/volume
 * pcr mastermix calculator
 * buffer calculator
 * pcr mastermix calculator
 * [[Media:Stammloesung.swf|Konzrechner]]
 * width=395px style="padding: 5px; background-color: #ffffff; border: 2px solid #C9D3EB;" |

white board

 * AG Quint - Lab Blog
 * DFG Science TV: Forscher stellen ihre eigenen Projekte vor
 * Naturally Obsessed: The making of a Scientist - documentary
 * historical papers of the royal society
 * professor science
 * free PC software for molecular biologists
 * Six Important Factors for Reverse Transcription
 * Video: Jede Zelle ...
 * Video: GFP-tagged HIV infiziert T-Zelle
 * The Large Hadron Rap
 * medicago gene expression atlas
 * the postdoc interview
 * craig venter's grand vision on synthetic genomics → video
 * Kits and their unique role in molecular biology: a brief retrospective
 * recycling electroporation cuvettes
 * pimp your plasmid growth medium
 * buffer calculator
 * Control Your Lab Computers From Home
 * run faster gels → new electrophoresis buffers
 * how-to-become-a-world-expert-in-your-field → artikel
 * quick and dirty screening for cloned inserts
 * awesome video → cellular visions: the inner life of a cell
 * Journal of Visualized Experiments


 * -valign="top"
 * style="padding: 5px; background-color: #ffffff; border: 2px solid green;" |

lab protocols
→ check also openwetware protocols

e. coli agros
 * cacl2-competent cells e.coli
 * e.coli transformation chemical
 * electrocompetent cells e.coli + electroporation
 * colony pcr
 * electrocompetent A.rhizogenes cells + electroporation
 * agrobacterium plasmid miniprep

arabidopsis
 * [[Media:southern.pdf | southern blot]]
 * arabidopsis dna isolation - quick and dirty
 * arabidopsis transformation
 * arabidopsis protoplast transformation
 * arabidopsis rna isolation protocols (including siliques)
 * transient transformation and expression
 * kanamycin selection of transgenic seeds
 * [[Media:basta.pdf | selection of transgenic seeds on basta plates ]]
 * ems mutagenesis
 * ats plates
 * iaa extraction and quantification

yeast → for general yeast handling follow Clontech Yeast Protocols Handbook → for Y2H follow Clontech Matchmaker Library Construction & Screening Kit
 * genomic dna
 * non-lethal ß-galactosidase assay
 * Short version ß-galactosidase assay
 * plasmid prep
 * yeast transformation
 * yeast western blot
 * mating in 24-well plates
 * yeast media

general
 * ligation (sticky ends)
 * northern
 * western


 * }