IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/08/06

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dspB Track

 * Transformants from yesterday taken out @ 1100

Protocol: Common protocols for PCR

PCR Cycles:
 * 95C @ 10min
 * Cycle 25x:
 * 94C @ 30 sec
 * 56C @ 30 sec
 * 68C @ 2 min
 * 68C @ 20 min
 * 10C @ hold

Start: 1204 End: 1351

Gel verification for colony PCR products
Gel orientation: Results: Vicki Ma 02:47, 11 August 2010 (EDT)
 * Protocol: gel verification protocol in Protocol (SOP)
 * Changes: 1% agarose gel
 * Machine conditions: 0.5x TBE buffer, 100V, 50min
 * Note: bottom results are QS Track's