User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/12/02

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Dissected 13 day heat shocked flies (2 x 2 hours @ 37 C)
 * Fixed 25' in 4% formaldehyde
 * Wash 3 x 5' in PBS-T
 * Incubate 30' in PBS - 0.6% Triton
 * Block O/N in 2% NGS


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