Kubke Lab:Research/CND/Records/MH001

=Embryo details=

Species: Gallus gallus domesticus Embryo Name: MH001 Embryo stage:28 Staging description: *Hamburger, V and Hamilton, HL (1951). "A series of normal stages in the development of the chick embryo". J. Morph. 88 (1): 49–92. doi:10.1002/jmor.1050880104. Fixation: PFA Cryoprotection: Yes cryoprotected by leaving the embryo in 0.1M PB 30% Sucrose solution overnight. Material label and storage: Labeled as MH001 and stored in a sucrose filled vial.
 * The limb-ends are a "pointer contour" or triangular as opposed to curved.
 * Beak or nose present therefore it cannot be a stage 27 when the beak is barely visible.
 * Forelimb and hindlimb has not reached the level of development in a stage 29. Justified by comparing the photograph as well as the description of the stage 29 embryo."Limbs: Wing bent  in  elbow.  Second  digit  distinctly  longer than  the  others."
 * Eye does not protrude out. (Unsure about this comment).

=Experiment details=

Objective:
 * Practiced cutting techniques which are described in detail here.

Procedure: Comments:

=Results= From this exercise I learnt that the a colder environmental setting facilitates less curling of OCT therefore making it easier to mount sections onto the slide. Cutting slowly also improves sectioning.

=Images= =Summary=