IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/09/Hao Wang

9:06 Miniprep the 2-4O->1-18P plasmids

11:52 Digest the plasmids of 2-4O->1-18P and 1-23L: Digestion System Insert: 20μL;

Digestion System Vector: 20μL;

12:13 Reaction Start

17:32 Stop the digestion

20:08 Prepare to Ligation

20:17 Ligation start