User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/04

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Check Overnight Growth BBa_J04450
Growth was fine

Miniprep BBa_J04450
Eluted in 35µl Water

Double Digest BBa_J04450 E|P
Eluted in 35µl Water

Gel BBa_J04450 E|P
1% Agarose Gel, 04-FEBRURARY-2011-17:44 GMT
 * 1) Lane 1: 10µL  1kbp NEB Quick Ladder
 * 2) Lane 2: BLANK
 * 3) Lane 3: 50µL BBa_J04450 E|P (Expected 1069bp (part) and 5263bp backbone (pSB1A10))
 * 4) Lane 4-8: BLANK



Purified BBa_J04450 E|P
Purified Second Band from top, expected 5263bp backbone

Ligation BBa__katE E|S & BBa_I13504 X|P & BBa_J04450 E|P
Setup a ligation containing
 * 1) 3µl BBa_J04450 E|P
 * 2) 1µl BBa_I13504 X|P
 * 3) 1µl BBa__katE E|S
 * 4) 3µl Ligation Buffer
 * 5) 1µl Ligase
 * 6) 1.5µl ddH2O

Transformation of Ligation
The whole ligation was transformed in an carbenicillin dish


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