Smolke:Protocols/Transformation


 * For each sample
 * 1 tube of electrocompetent cells (keep covered in ice at all time)
 * 1 electroporation cuvette (keep on ice)
 * 1 1.5-ml microfuge tube
 * 1 mL SOC
 * 1 LB plate with appropriate antibiotics
 * Gene Pulser setup
 * Exponential
 * 2000 V voltage
 * 25 uF capacitance
 * 200 ohms resistance
 * 1 mm cuvette
 * If transforming non-purified, non-concentrated ligation product, use 0.5-1.0 ul per sample.
 * Time constant should be at least 4.5, generally between 4.7 and 5.0.
 * If sample sparks or has a low time constant, lowering the volume of ligation product is generally more useful than increasing the volume.