IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/02/12

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 * style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]] iGEM Project name 1
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Objective

 * Get an idea of final construction.

Looking for:

 * Assembly of all necessary bio-bricks (are they already assembled?)
 * Looking for bio-bricks and methods
 * Protocols (strain, reactants, medium)

Bio-bricks must contain:
 * We need to prove if it is possible for a luciferase emitter to stimulate a chromophore receptor.
 * Plasmid structure
 * Vectors must have the same structure, this structure is an iGEM standard, all bio-bricks have the same standard unless specified.
 * Standard assembling


 * Suffix and Prefix:

These are sequences placed at both sides of the sequence to be inserted; these sequences contain several restriction sites in order to excise the bio-brick if necessary. It is important to notice that the principal sequence placed between the suffix and prefix sequences must lack restrictions sites contained in suffix/prefix sequences to avoid bio-brick damaging.
 * Origin of replication


 * Expressed sequence

RBS (Ribosome binding site)

Promoter

Gene

Terminus


 * Resistance gene

The sequence to be expressed should be like this:

Suffix--rbs—promoter—ATG—prot—stop codon--prefix


 * We need to search for bio-brick’s specifications like kind of restriction-sites, resistance gene, bio-brick’s length, etc.

Modeling
It is necessary to model:
 * Signal input
 * Transcriptional response after receptor stimulation depending on the number of photons received.
 * How strong was the transcriptional response after a defined photon input.


 * What do we expect of our model?
 * Modeling signal input and transcriptional response.
 * We need to model: elements, circuit, parameters, logic design.