Hoatlin:Crosslinking antibodies to beads


 * 1) 400μL Protein A Sepharose Fast Flow Beads (GE Healthcare #17-1279-01)
 * 2) Rotate @ room temp (RT) 30 min-3 hrs (3 hrs @ 4°C)
 * 3) Wash 4X in PBS (1ml for each wach)
 * 4) Wash 2x in 100mM  Na Borate, pH 9.0
 * 5) Resuspend beads in 1 ml 100mM Na Borate, pH 9.0 + 5.2mg DMP (Dimethyl pielimidate, Pierce #21667)
 * 6) Rotate 45 min (don't exceed 1 hr) at RT
 * 7) Spin 1 min at RT and recover beads
 * 8) Wash beads 1x w/ 1M ethanolamine, pH 8.0 (Sigma #E9508)
 * 9) Add 1 ml of 1M ethanolamine, pH 8.0
 * 10) Rotate 2 hrs-overnight at 4°C
 * 11) Wash 4x quickly in 100 mM glycin pH 3.0
 * 12) Neutralize by washing 2x w/ PBS
 * Store at 4°C
 * Beads can be used several times