User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/03

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] ELISA, RII, IF
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * ELISA (Caro (12:08) & Mine (11:55))
 * Mine was the same as the previous (01August2010)
 * NOTE: FORGOT TO ADD DMSO TO THE ONES NOT RECEIVING Ht31!
 * New cells were plated out, D9 P23
 * RII Overlay showed NOTHING!! Not even marker signals...
 * Most probable cause, blot was incubated ON up side down?
 * IF Cells seeded in 100.000 cells per plate are swarming the coverslips, only use 75.000 cells from now on


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