Griffin:Zinc-Fixation

Zinc Fixation Protocol
Zinc-Fixative:
 * 1000 mL 	0.1M Tris buffer, pH 7.4
 * 0.5 g		Calcium Actate
 * 5.0 g		Zinc Acetate
 * 5.0 g		Zinc Chloride

Mix to dissolve, do not adjust pH (it should be about 6.5-7.0).

I) Place freshly dissected tissue (3 mm or thinner) in Zinc-Fixative and allow to sit at room temperature 6-48 hours.

II) Dehydrate tissues as follows, all at room temperature:


 * one hour 70% ethanol
 * one hour 95% ethanol
 * one hour 100% ethanol
 * one hour 100% ethanol

III) Clear tissues as follows, both at room temperature:


 * 50 minutes in Xylene
 * 50 minutes in Xylene

IV) Follow with paraffin embedding.


 * 1) Paper1 pmid=12808124