User:Meng Xiao He/Notebook/fall08/2008/10/30

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PCR redo


7-11 should be: 12???????
 * cbb3III 5
 * cbb3I 3
 * ccoIII 3
 * DuoII 5
 * DuoI 3

use columns 4, 6, 9, 11 on gradient mxfg, with dmso in 11 (.3ul)

clonewelled

Digest
BsmBI, overnight at 55, heat killed at 80 for 25min:
 * ccb3 (both)
 * cco (both)
 * Duo (both)

Rx:
 * 15 ul of each PCR clonewell frag
 * 3.55ul buffer 3
 * 2ul BsmBI


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