User:Karmella Haynes/Notebook/BioBrick cloning/2009/11/19

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11/19/09

 * &#x2713; Minipreps: KAH77(2), 78(3), 79(3), 83(2), 84(2), 85(2)
 * &#x2713; Assemblies: NLS myc stop (KAH011), K-stop (KAH012); Postpone ---> KAH93, 94, 95
 * &#x2713; Cultures: KAH86(2), 87(2), 88(2), 89(2), 90(2), 91(2), 92(2); KAH84(2, re-pick); 5 ml ea.

Minipreps > Check with E/P digests (Fermentas FD)

Note: Re-do KAH77-79 digests tomorrow, triple digest to cut V0120

Assemblies (tomorow)
 * 1) KAH011: NLS-myc-stop/(X/P ds-oligo)/64 + V0120/(X/P)/3200
 * 2) KAH012: Kstop/(X/P ds-oligo)/24 + V0120/(X/P)/3200
 * 1) KAH93: 2xHS4-Ubc-K-Gal4DB-PEST-2xmCh-NLS-stop (KAH77)/(E/S)/3480 + 5xLuc target-polyA-2xHS4 (KAH67)/(E/X)/4553
 * 2) KAH94: 2xHS4-HPK-K-Gal4DB-PEST-2xmCh-NLS-stop (KAH78)/(E/S)/3652 + "
 * 3) KAH95: 2xHS4-CMV-K-Gal4DB-PEST-2xmCh-NLS-stop (KAH79)/(E/S)/3724 + "

> Ligations


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