User:Vijay/Protocol/urease-assay

Overview
Urease agar assay is used to differentiate micro organisms based on their urease producing ability.

Materials

 * BBL Urea agar base concentration 10X (Becton, Dickinson and Company (BD), Sparks, MD)
 * Granulated agar

Procedure

 * 1) Dissolve 1.7g of granulated agar in 100 ml of dH20.
 * 2) Boil it for 40sec in a microwave
 * 3) Autoclave the boiled agar at 121C, 15min, 15lb
 * 4) Cool the agar to 50C
 * 5) Add 10ml room temperature Urea Agar Concentrate-10X to the agar
 * 6) Pour in to plates (5 plates)
 * 7) Streak wild type, mutant on to the plate
 * 8) Incubate wild type, mutant and a control at 37C
 * 9) Watch for change in the color from yellow to pink
 * 10) A pink color indicates presence of Urease activity