User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/29

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Trying to handle as many things as possible!!!
  -H2O > 7μL
 * Today, I checked the gel of my restrictions and as I observed the correct size in a reasonable concentration, I ligated them using the following reaction for a total of 20μl:

-Buffer ligase --> 4μL

-Vector (plasmid) -> 2μL

-Insert (luciferase) ---> 6μL

-Ligase -> 1μL   
 * I left the ligations at 16°C ON.


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