IGEM:IMPERIAL/2007/Week5

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This Week's Aims

 * Wet lab: Cheuk, Maira, Ben
 * Cloning:
 * sort out DNA constructs to build
 * transform cells
 * amplify biobricks
 * midi/maxi preps
 * digestion and ligation
 * build complete DNA constructs
 * In vitro:
 * wait for materials/look for BL21
 * prepare cell extracts

By end of week: Build complete DNA constructs


 * Merge protocols: James, Peixuan, Lucas
 * Define standard units
 * Learn chassis
 * Learn cell-by-date
 * Learn biofilm
 * merege protocols (prioritise)
 * provisional lab schedule
 * learn biofilm production

By end of week: Efficient planning pf protocols and lab sessions


 * Learn vessicles: Dirk, Cheuk
 * Read up papers
 * materials
 * equipment
 * compare results of different techniques
 * Contact people
 * focus on techniques
 * ask for opinion

By end of this week: Finalise protocols, Wait for material delivery


 * Wiki: Everyone
 * Have a schedule
 * weekly
 * daily
 * Updated project page
 * progress throughout each day
 * progress throughout each week
 * Have an iGEM 'digest' for the professors and supervisors (email)

By end of week: Get supervisors back into the 'loop'. Get organised


 * Modelling: Jerry, Alex
 * Learn biofilm
 * diffusion process within biofilms
 * [AHL] and temperature dependence
 * Learn cell-by-date
 * temperature dependence
 * degradation time
 * steady state (rate of formation/decay of GFP)

By end of week: Model basic constructs and parameters to be measured

Expenses
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