IGEM:MIT/2006/Notebook/2006-7-31

to do

 * 1) find a new glycerol storage box and transfer 7/29's new glycerols into it -done
 * 2) nanodrop 23 minipreps from 7/29 and prepare 46 small tubes for sequencing order -done
 * 3) *sequence results from tubes 23/24, 27/28 have been discarded b/c we do not have enough dna
 * 4) troubleshoot pchBA (w/out silent mutation) PCR
 * 5) digestions+ligations+transformations (design a plan)
 * 6) get ready to fax offices about p. shuttle vectors -done, need Isadora
 * 7) link our system device diagrams on main wiki page -done

Current products (sequence results to verify will come in on wed 8/2)

 * 1) RBS-CDSBSMT-Term
 * 2) RBS-ATF1mut
 * 3) SPP-RBS-GFP-Term
 * 4) ATF1mut-Term

Digestions (and gel extractions)

 * 1) RBS30, 32-CDS-Term: XP
 * 2) RBS30, 32-ATF1: ES
 * 3) osmY: SP
 * 4) ATF1+Term: XP
 * 5) R0040: SP
 * 6) E0840: XP

Digests needed for ligation in our possession

 * 1) B0015: EX
 * 2) B0030: SP
 * 3) B0032: SP

Gel Extraction

 * 1) RBS-CDS-Term: XP 30ABC came out
 * 2) E0840: XP also came out
 * 3) RBS-ATF1: ES30/32 AB

pchBA PCR
Ran pchBA PCR with gradient -- unsuccessful again. We need advisor help.

BAT2 and THI3 PCR (and gel)

 * 1) primers recieved, 55 degree/standard PCR program

Sequencing Results
Looked at R40 + B30/32 sequencing results. Seems that the promoter is missing and we will be going with different set of ligations.

liquid cultures

 * 1) R0040
 * 2) osmY+E0840(A,B,C)
 * 3) ROO30+ATF1mut(A,B,C)