Stanford/BIOE44:Module 1:Day6

=M1: Day 6 - Arsenic!=

How to find and get parts

 * 1) Search the parts registry.
 * 2) When you find a part you want, click on the "get this part" link located on the right.
 * 3) If the part is in the 2009 or 2007 distribution, we should have it (but there is a small chance we won't if it has already been used).
 * 4) Send an email to Isis with the following information. All of this info is listed on the 'Get Part' page!:
 * 5) *Part number
 * 6) *Distribution (ex. Spring 2007)
 * 7) *Plate number
 * 8) *Well number
 * 9) *Antibiotic resistance
 * 10) Isis will send you a confirmation email containing which parts are available to you.
 * 11) Isis will transform the parts into E. coli. You will receive a plate with colonies on it. This means it will take at least a day to get your parts.

Some interesting results for those doing a colony PCR


1% and 0.8% agarose (90V)

What can you gather from this gel?

Summary of Procedures


 * 1) Colony PCR, arsR biobrick primers, MG1655
 * 2) PCR Cleanup Qiagen
 * 3) Digestion with EcoRi and SpeI
 * 4) Method A: PCR Cleanup Kit only
 * 5) Method B: PCR Cleanup Kit + Gel Extraction