User:Tk/Notebook/MF/2009/11/08

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Mesoplasma florum simplification
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Resequencing L1, PP, GF, W12

 * Growth after 24 hours showed differences in colony diameter
 * L1: 6-7 divisions about 100 microns
 * PP: 4-5 divisions about 75 microns
 * GF: 2-3 divisions about 40 microns
 * W12: no visible colonies, possible contamination with yeasts
 * sterile control shows no growth


 * Scale is 16.5 microns/division


 * pick single colonies of L1, PP, GF into two 10 ml cultures
 * Streak out 3/25/09 culture of W12

Annotation

 * M. pneumoniae phosphatase mpn248 is required for tip assembly, PMID
 * M. florum has genes mfl220 and mfl221 homologous to mpn247 and mpn248
 * M. florum genes are dispensable


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