Template:SBB10 ConstructionFiles JennaK sbb29

Biobricking of 099 rep protein basic part sbb29

PCR repo99-F and repo99-R on pEC52 gene            (978 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144                          (EcoRI/BamHI, 918+2472, L) Product is pBca9523-repo99			     {rbs.repo99!} repo99-F  Cloning of repo99   ccaaaGAATTCtaaAGATCTcctggaaggaatcgtaacac repo99-R  Cloning of repo99   gctagGGATCCctactctacaagacctcg



JCA Notes
Biobricking of 099 rep protein basic part sbb29
 * Prefix should be ATG not TAA
 * Vector size slightly off
 * Reverse oligo can be shared with other part
 * Plasmid name should be partName, not partDescription

PCR repo99-F and JG002-R on pEC52 gene            (978 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144                          (EcoRI/BamHI, 910+2472, L) Product is pBca9523-sbb29	{rbs.repO99!} repo99-F  Cloning of repo99 ccaaaGAATTCatgAGATCTcctggaaggaatcgtaacac JG002-R  Cloning of  O99 rep Gene CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc