BISC 219/2009: Mod 3 Experiment 1 Media Recipes

Media Recipes
MS4 = BASAL MEDIA Murashige-Skoog (1969) defined plant tissue culture media 4.42 grams/liter MS media complete(Invitrogen) or make by combining: Nitrates 165g/l NH4NO3 190g/l KNO3 Sulfates 37g/l MgSO4.7H2O 1.69g/l	MnSO4.H2O 0.86g/l ZnSO4.7H2O 0.0025g/l CuSo4.5H2O Other 44g/l CaCl2.2H2O 0.083g/l KI 0.0025g/l CoCl2.6H2O 17g/l KH2PO4 0.025g/l NaMoO4.2H2O 2.784g/l FeSO4.7H2O 3.724g/l Na2 EDTA Vitamins: 5mg/ml nicotinic acid, 4mg/ml thiamin HCL, 5mg/ml pryidoxine HCL 30.0 grams/liter sucrose 6 ml/liter of 0.5M MES, pH 5.8 1mg/ml glycine 100g/l myo-inositol MS4 = SHOOT INDUCTION MEDIA Murashige-Skoog defined plant tissue culture media with: 0.3 mg/liter indoleacetic acid (IAA), an auxin, 10 mg/liter dimethylallylamino purine (DMAP), a cytokinin. MSP2 = ROOT INDUCTION MEDIA Murashige-Skoog defined plant tissue culture media with: 0.1 mg/liter napthalene acetic acid (NAA), an auxin.