User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/04/13

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Tuesday 13th April 2010
I made 1 lt of LB solid medium using:

Peptone              10g

Yeast extract     5g

NaCl                     10g

Agar                     15g


 * Procedure:


 * Weigh the ingredients but the agar and place them into a flask


 * Add 250ml of water to dissolve and shake until it looks even, meanwhile place 3.5g of agar in 4 schott bottles


 * Fill in a graduated cylinder with the mixture of the ingredients and water up to 1lt.


 * Add 250ml of the mixture with all the water to every schott bottle, then put the in the sterilizer to melt.


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