User:Tk/Notebook/MF-xfm/2008/04/22

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Construction plasmid PCR

 * 100 ul final
 * 50 ul Phusion mastermix
 * 1.5 ul suffix-FB
 * 1.5 ul prefix-RB
 * 0.5 ul of plasmid (pSB1AC3, 1AK3, 1AT3)
 * 46.5 ul water
 * Cycle 98/30s 32x(98/20s 55/20s 72/2min) 72/10min

Transformation results

 * Picked 40 colonies off of 4/20 transformations
 * +10 worked best, 0 worked well, -5 few, -10 none, +15 few
 * replated 0,-5 saved cultures

Construction plasmid distribution

 * Each team gets 300 ul of each plasmid
 * Concentration is 10 ng/ul
 * Each team needs 3 ug of plasmid
 * With 150 teams, we need 450 ug of each plasmid


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