Maheshri:Duties

=Long-term Duties=

EHS Rep
Currently: Shawn Finney-Manchester

Computer Guru
Currently: Nick Wren

Web/Wiki Master
Currently: C.J. Zopf

Maintenance
Currently: Tek Hyung Lee

Inventory
Currently: Katie Quinn

Social Chair
Currently: Bradley Niesner

=Rotating Duties (2-month rotation)=

Ordering

 * Every day, items that are written on the white board in the office should be ordered and a check placed next to the item. When the item arrives, erase the item from the board.
 * When items arrive, stock the shelves and bring the packing slip to the Administrative Services Office in 8-328 (weekly at least).
 * Once a week, the following stocks need to be checked and any low or missing items should be ordered. A printable list of the commonly ordered items below and their VWR catalog numbers is available here.
 * For urgent supplies, any catalog number followed by "SR" can be purchased in the stockroom in 56-070 (though the catalog numbers often represent bulk packages, the stockroom usually has smaller quantities or different brands).

Lab Supplies

 * Common enzymes should also be checked once a week, and most can be purchased through the stockroom. The enzyme boxes in the -20 are labeled with all common enzymes on the lid. If we are out of an enzyme, its well will be empty. Any duplicate unopened enzymes should remain in their packages and be placed next to the enzyme boxes. Order the HF (high fidelity) version of the enzyme if it exists to facilitate double digests.

Autoclave Biological Waste
The non-sharp biological waste bags need to be removed from their bins and autoclaved before they can be thrown in the regular trash. See the protocol here

Liquid media
Common media and solutions to make and autoclave:
 * YePD
 * 2xYPAD
 * TB
 * SD min
 * 50% glucose solution
 * ddH2O

Plates
Common media plates to make:
 * TB-Amp
 * YePD plates

Autoclaving tips
Autoclave and stock shelves
 * pipette tips (10μL, 200μL, 1000μL)

Autoclaving can be done on dry cycle: 20 min sterilize/20 min dry

Dishwashing
Either by hand or using dishwasher in the autoclave room, wash the common dishes:
 * Media/solution bottles and caps
 * Erlenmeyer flasks
 * Test tube caps

Autoclaving culture tubes and flasks
Autoclave to restock sterile: Autoclaving can be done on dry cycle: 20 min sterilize/20 min dry
 * Test tubes with clean caps (13x100mm blue cap, 16x150mm green cap)
 * 250mL Erlenmeyer flasks with aluminum foil caps

Autoclaving small items
Autoclave and stock shelves: Autoclaving can be done on dry cycle: 20 min sterilize/20 min dry
 * microcentrifuge tubes
 * toothpicks
 * applicators
 * 96-well deep well plates