User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/09

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Gravin Co-IP, cAMP precipation gel
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * AKAP250 immunoprecipitation
 * Gradient (4-12%) SDS-PAGE (NuPage) for cAMP Precipitations

Results

 * Overnight autoradiograph of RII overlay did not change anything of the image of yesterday
 * Ponceau of cAMP pull down SDS-PAGE

Discussion

 * Ponceau shows bands at higher altitude, maybe look for AKAP-Lbc next to AKAP250?
 * Anoying that both markers are not running equal on every height, but HiMark at least has nice sharp band @ 160 kDa on NuPage gels. Although transfer was less perfect
 * Coomassie staining shows not a 100% efficiency of transfer, and it shows bands in the control lanes that were not visible on Ponceau staining. Hopefully something did transfer


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