SBB09 Construct39

Construction of  basic part Bcr0002

PCR Ocr0001-F/Ocr0002-R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-Bcr0002 {a~Inv-long>} - Ocr0001-F      forward oligo for biobricking of {a~Inv-long>} ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg Ocr0002-R      reverse oligo for biobricking of  {a~Inv-long>} ccaaaGGATCCctgctggcctcAagactggatacc

JCA: Use Douglas's Odmd002R for your reverse; you need to use the M numbers for naming

Construction of  basic part Bcr0002

PCR Ocr0001-F/Odmd002R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10031 {a~Inv-long>} - Ocr0001-F      forward oligo for biobricking of {a~Inv-long>} ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg Odmd002R	Reverse Oligo for Invasin (refactored, long) ccaaaGGATCCactggataccagcgatctgccg