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James Macaulay



lambda red/recA part jtk2979 

Lambda red/recA exists but needs to be moved
The lambda red genes encode proteins that enable efficient homologous recombination in E. coli. Many publications have described its use in genome manipulation experiments, and one of the more useful ones is PMID 10829079. Unfortunately, it is somewhat toxic to E. coli, and we want to know why and hopefully alleviate the problem. A composite part encoding all 4 CDS in one operon with ribosome binding sites and terminator but no promoter as part jtk2979. However, it is associated with the wrong vector (plasmid pBca9145-jtk2979). We need to move it into a new plasmid with vector pBca1766.

P_hdeAB part sbb1128