User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/05

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Co-IP day III, Stop stimulation hTERT, Split hTERT
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Short summary

Method

 * Co-IP day III
 * Stop stimulation D9/D12 24-wells induced yesterday (after 24 hours)
 * Alamar Blue
 * .. D9 ELISA samples got lost, D12 was put to 96-wells in freezer
 * Split cells D9/D12
 * 30.000 cells per well for 24-wells
 * 200.000 cells per well for 6 wells
 * 1 million per plate for 10cm petridish

Results

 * Viability staining


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