Yu:quick miniprep

Procedure

 * 1) Spin down 1-3 mL culture
 * 2) 4000 RPM for 5min at 4°C
 * 3) Transfer pellet to eppendorf tube if not already in one
 * 4) Resuspend pellet in 200 μL P1
 * 5) Add 200 μL of P2
 * 6) Mix by inversion - solution will become very viscous
 * 7) Add 200 μL of P3
 * 8) Mix by inversion - white precipitate will form
 * 9) Spin mixture for 10 min at top speed
 * 10) Remove pellet with a sterile toothpick
 * 11) Add 600 μL Isopropanol
 * 12) Spin mixture for 3 min at top speed
 * 13) Decant supernatant
 * 14) Add 500 µl 70% EtOH and vortex briefly
 * 15) Spin mixture for 3 min at top speed
 * 16) Decant supernatant
 * 17) Air dry pellet
 * 18) Resuspend pellet if 50-100 μL TE8

P3
3M NaOAc pH4.8