WangLab:Ligation Samples

Two piece ligation

 * 5X ligation buffer: 	4
 * Vector: 		1
 * Insert: 			3
 * T4 ligase: 		1
 * H2O: 			11

Three piece ligation

 * 5X ligation buffer: 	4
 * Vector: 		1
 * Insert 1: 		5
 * Insert 2: 		5
 * T4 ligase: 		1
 * H2O: 			4