User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/25

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

{| width="800"
 * style="background-color: #cdde95;" align="center"|
 * style="background-color: #cdde95;" align="center"|




 * align="center" style="background-color: #e5edc8;" |

title=Search this Project


 * colspan="2" style="background-color: #F2F2F2;" align="right"|Customize your entry pages 
 * colspan="2"|
 * colspan="2"|
 * colspan="2"|

June 25th, 2010
I dephosphated the two vectors I'm gonna use: plasmid J61002 carrying constitutive promoter J23106 and ampicillin resitence and plasmid 17. I followed the next protocol to dephosphate the two plasmids:

15μL DNA

3μL Buffer

1μL phosphatase

11μL H2O

30μL TOTAL

Then I made an agarose gel in order to make the ligation

LuxAB - Plasm 17 - Plasm J61002


 * }