WangLab:Confocal Microscopy Usage

Confocal Microscopy Processes:
Note
 * Do not touch the fiber cable for Laser!
 * If you want to change objectives, see Jerry!


 * 1) Turn on Arc Lamp first.
 * 2) Turn on Laser Power, Make sure the laser is on Standby mode (Standby light should be on). Turn the key to On->Start->On on laser.
 * 3) Turn the key on the laser set besides microscopy to On (Off->Spinning->On).
 * 4) Turn on computer, Turn on camera, turn on controllers for Stage, filter shutter
 * 5) Get into Simple PCI, Select file->manage profiles->Simple PCI 5.0
 * 6) Click on the camera icon, Choose Monochrome for single staining (you can choose other options based on your exp.), Select filter set, for FITC, it is 488/525.
 * 7) Only one small drop of oil on lens (must be Olympus oil), mount sample slide, Turn on the regular light lamp, focus the sample with eyepiece.
 * 8) Turn off regular light, switch to SP (side port?).
 * 9) Turn the switch from Standby to On on the shelf laser controller.
 * 10) After experiment, turn the key on the shelf to Off, the key besides the microscopy to Off (On->Spinning->Off).
 * 11) Lower Objective, take off sample, clean lens (wipe off the oil excess with dry lens paper, and clean the lens with lens paper with lens cleaning solution).
 * 12) If the noise from laser is gone, turn off the power switch of laser on the shelf.
 * 13) Turn off light lamp.
 * 14) Change simple PCI profile to Null
 * 15) Turn off camera, controllers for shutter and stage.
 * 16) Turn off computer after image analysis.