IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-27

Secondary antibody incubation
Incubated for 45 minutes, then transferred the liquid to tube labeled "IRDye 700" for reuse.

Final wash schedule

 * 2 10m washes of PBST
 * First wash started: 1050
 * Second wash started: 1216
 * 2 10m washes of PBS
 * First wash started: 1230
 * Second wash started: 1243 --note: we washed for only 5m due to time constraints
 * Rinse with distilled water when finished
 * Wrap in aluminum foil, store in 4C refrigerator until imaging

Anybody want some data?