IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-11-20

Gel Analysis (repeat)
- samples: c5.0A (no lids, no aptamers), c5.0C (no lids, inside aptamers), c5.0D (no lids, outside aptamers)

- 2.0% agarose gel: 2 g agarose + 100 0.5x TBE + 1.1 mL 1 M  + 5  EtBr

- lanes: 1 = ladder (10 ) 2 = p7308 scaffold (5 + 3  dye) 3 = c5.0A (5 + 3  dye) 4 = c5.0C (5 + 3  dye) 5 = c5.0D (5 + 3  dye)

- run 1 hr. @ 130 V



- results: the folding did not succeed, as can be seen by the lack of bands in the 3rd through 5th lanes near the position of the scaffold band