User:Wayne G. Shreffler/Notebook/Daily Log/2009/06/02

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*Wayne G. Shreffler 23:25, 2 June 2009 (EDT):

 * Orientation for Priya in morning
 * needs novell/ email
 * needs accounts


 * R course lesson 2
 * Need to create assignment for next week that involves editing an existing figure


 * Met with Carl -- Project will focus on CCL22 effect on basophils
 * Does CCL22 (or CCL17) induce RALDH2? can test using Aldefluor
 * Does CCL22 activate basophils to alter Th differentiation? co-culture basos and T cells, activate T cells by anti-CD3/-CD28


 * Met with Caitlin -- will focus on following experiments:
 * DHR with CD63 and pErk following anti-IgE to look at function of endo H2O2.
 * straight desensitization with anti-IgE and fMLP in which sups are saved for histamine measurement.
 * kinetics of DHR following stim
 * dependence of peroxide-induced desens on src kinase (use inhibitor)


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