BCH070505-Construction of pJ23006-J23009

[EcoRI][TT][Ptet][XbaI][Ptet][RFP][TT][SpeI][PstI]+[XbaI][Key3d][SpeI][PstI] Extend KB007/KB008 and digest                  (XbaI/PstI, Neb2, 126bp, use small frag cleanup) Paste into pJ23006                 (XbaI/PstI, Neb2, Large-2334bp) Product is pJ23006-J23009