Klapperich Lab:Notebook/Lab Meeting Notes/2009/03/05

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5 March 2009 Lab Meeting Agenda
Bioanalyzer DONE Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look
 * Status: compressed air and vacuum. DONE
 * Co. says that it works fine. Transducer up to 1000 psi. We are getting it back.

† Announcements † Flu R01 † SEPSIS Project †RNA project † COBRA - Discussion for different approach using COP film as their substrate - Try glass fixture. - magnets hold Teflon substrate in place and make sure they sink - they crack as soon as left dry - nanopatterned COP provides access to repeatable morphology  *try EBEAM gold on COP - Engineered COP substrate? - Do Rhodamine. -Need a size specific positive control. PS beads? charged? † Valve Array/Valve building - done.
 * MicroTAS abstracts.
 * Talked to Patty and Jim today. Need more sample. No more splitting. Compare notes with Sonali, Patty and Jim
 * 3/13 Cathie meeting with Nira Pollcak - more samples
 * BUMC _ John Connor - Cathie working on IBC amendment. Two transportation steps
 * BUMC _ John Connor - Cathie working on IBC amendment. Two transportation steps
 * Submitted to Integrative Biology.
 * New proposal with Fraunhofer for food due this week.
 * New proposal with Fraunhofer for food due this week.
 * Integration test from here forward.
 * Test ongoing with Rubber adhesive tape for Sol-gel.
 * Test with engineered SERS substrates
 * Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann.
 * benchtop or floor models searched, price range 32K+, waiting on quote.
 * Substrates (JZ)
 * three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD)
 * Colloid experiment.
 * ordered
 * 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
 * co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles

† Fraunhofer: LOAC † Biointerfaces group † CIMIT- Colson Grant † PCR † RCA/HDA † Senior project † F31: Cochlea
 * Paper drafting!
 * Final experiments were done.
 * counting the number of trapped cells
 * adding experimental results in the draft paper.
 * adding experimental results in the draft paper.
 * IRB still in revision.
 * Cathie in charge of PCR1 paper right now.
 * Developed a new protocal for PCR coating
 * Simulaons of DNA chain model are running (60 beads).
 * Cathie submitted One pager Whitepaper. Waiting for response.
 * convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested?
 * PReliminary no-valve design for 25 microliters.
 * 20 more test chips to Sonali (AACC poster).
 * Sonali try to do 10 microliters in the tube
 * Megan
 * Test on Thu, Fri worked but failure after some cycles.
 * Need to enhance bonding (e.g. Ar or O2 ashing)
 * Cell/matrix paper blocking controls. Likely final questions... when is Decorin up/down regulated--- is there an association between fibronectin and actin cytoskeletons during regeneration?  Obtained feedback on culture paper.

† Silica Optimization (Lambda):
 * Do full spectra on dirty controls - with PG and Without PG
 * try another color DNA dye.


 * UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday 

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html


 *  reading about all the different stains to 

Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


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