User:Karmella Haynes/Notebook/BioBrick cloning/2011/06/01

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06/01/11

 * &#x2713; Cultures/minipreps/digests: assemblies from 5/31/11 (2 each)
 * &#x2713; Assemblies: re-do KAH188, 190; new KAH191, 195, 196, 197, 198

Minipreps > Check with E/P digests

--> Re-do KAH188, 190 (to get better cloning ratio, less background, use less vector)

Assemblies
 * 1) KAH188: KAH83/(E/S)/443 &#x2713; + KAH182/(E/X)/~4875 &#x2713;
 * 2) KAH190: KAH85/(E/S)/687 &#x2713; + "
 * 3) KAH191: (1) KAH185/(E/S)/size + "
 * 4) KAH195: (2) KAH193/(S/P)/~5988 + polyA2/(X/P)/225 &#x2713;
 * 5) KAH196: " + KAH171/(X/P)/418 &#x2713;
 * 6) KAH197: (3) KAH194/(S/P)/~6232 + polyA2/(X/P)/225 &#x2713;
 * 7) KAH198: " + KAH171/(X/P)/418 &#x2713;

> Digests (Fermentas FD) --> Specific notes

> Measure conc.'s

> Ligations

--> Add total lign. to 30 μL DH5α-turbo


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