Knight:RNA work



A list of various items that might be useful while working with RNA.

The sterile hood in 306 is dedicated to RNA work.


 * 1) RNaseZap (Ambion)
 * 2) RNaseZap wipes (Ambion)
 * 3) DMPC (less carcinogenic than DEPC)
 * 4) RNaseAlert Kit (Ambion)
 * 5) RNase free water
 * 6) *The DI water may be sufficiently free of RNases
 * 7) 3% Hydrogen peroxide
 * 8) Oven for baking glassware
 * 9) *Note that caps can be heated only to 140&deg;C but removal of RNAases requires 180&deg;C
 * 10) Decontaminated electrophoresis apparatus and power supply
 * 11) RNA ladder
 * 12) SYBR Gold
 * 13) *Sensitive nucleic acid stain.
 * 14) RNase free 10X TBE buffer
 * 15) *For native gels
 * 16) Glyoxal and formamide
 * 17) *For denaturing gels
 * 18) RNase free tips and tubes
 * 19) RNase free DNase I (We have Deoxyribonuclease I from bovine pancreas. Is this good enough for RNA work?  Stored at -20&deg;C in the large freezer on the door shelf.)