Matt Gethers/CRI, Thailand/Labwork/HmgR Purification Protocol Using Heparin/7.28.09 SDS-PAGE Results

=SDS-PAGE to Assay 7.28.08 Elution Fractions=

Gel 1

Gel 2

I don't see any sign of an ~28 KDa protein on the gel. The -/+ IPTG samples from the culture look identical, so I don't think I'm getting any induction. There's plenty of protein in the post-sonication pellet and the flow through, a little bit in the first fraction of the wash through, and nothing much in the last fraction of wash through. In each case, there is protein along the length of the lane, no real peaks. Fractions 3 - 5 of the elution have several bands, again none of which are ~28 KDa. Fractions 6-14 also have several bands, though there appears to be a recurrent band at ~100 KDa.