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Dorothy Tulanont

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sbb26: P9 regulatory RNA
Source: pEC22-P9 Target Sequence: tgaagtgaccggattagcaacgcagtaacccgaaatcctctttgacaaaaacaaagcgtgtcaggctgattctgatgcgcttttttttgaaatgtcacaaaaattccatgtgggagatgggatctaaaatccccgtgcagaactttctatccagggggagaaaacttgtcgttttgagccgttcggtgttcagaacgcacgaaaccgatcgcgcatcgctttcgtgcatagttatgcaacgcgctaaaaacgattctgacgcattttttatgtttaccctggtgtttccttgtctttttgcgttttttgcgtcagaaagcgattgagggcgttttaaggggggcgtacaacgggagttatggtaaatggatcgggttttcgggaaggatcgacaggatttgccgttgggtgtagtgtaagcgactgaaaaacaaacgccccgtaaatcgtgctctcaccgccaagattgatcacgaaattacagggcgccgggttccgcgtttcccgatgggaaagcgcggttagttaaactgtgtaccgagagaaatcgtatcacatgagcgccgtacttcaacgcttcagggaaaaattaccgcacaaaccgtactgtacgaacgatttcgcgtacggcgttcgcattctgccgaaaaacattg Vector: pBca9523 Short description: P9 5'UTR Genbank reference: D30054 (1…656) Family: Expressed RNA

sbb25: CA42 regulatory RNA
Source: pEC22-CA42 Target Sequence: cagctgaagtgaccggattagcaacgcagtaacccgaaatcctctttgacaaaaacaaagcgtgtcaggctgattctgatgcgcttttttttgaaatgtcacaaaaattccatgcgggagatgagagctaaaatccccgtgcagaactttccacccagggcgagaaaacttgtcattttgacctgttcgcccttcggaacagtcgaaatcgatcgcgcatcgctttcgtgcatagttatgcagccctctaaaaacgattctggcgcgtttttctggtttggcctggtgttttcttgtctttttgcgttttttgcgccagaaagcgactgaggggcgttttaaggggtacgtacaacgggagttatggtaaatggatcgggtttycgggaaggttcgacaggatttgccgttgggtgtagtgtaagcgactgaaaaacaaacgcccctgaaatcatgtccagatccggcaagattcaacatgaaatacagagggcgtgtaggtcgcataacccggagagaatcgtaacacatgagcgccgcgcttcaatacttcgacgaaaatttaccccatcgtccctatcacacggatgatctcgcttttggtcttcgcatctccggcaaagggcgtgc Vector: pBca9523 Short description: CA42 5'UTR Genbank reference: D30056 (1…630) Family: Expressed RNA

sbb43: Pre-Pro sequence
Source: pBjh1601AC-Bjh1730 Vector: pBjk2741 Short description: {rbs2_lamB>} Family: Targeting Peptide This part encodes a prepro sequence. The "pre" sequence targets secretion of a protein fused to it's C-terminus to the periplasm of E. coli. the "pro" part of the sequence allows the peptide to be clipped off by a protease releasing the protein to be a soluble protein in the periplasm. Note that this part is of the rbs.part> flavor, so it also encodes a ribosome binding site and start codon.