User:Linh N Le/Notebook/2009/06/12

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Questions for Andy

 * Andy asked Brigette and I to come up with questions pertaining the experiments we ran last night (AA + MT + AF)
 * The bulk of the questions, for me at least, will deal with some chemistry and pH related things

The Questions

 * 1) What pH do MT's thrive in? (my guess would be near 7)
 * 2) What is the pH of BRB80 (and BRB80T)? (I overheard it was made of salts, so possibly near 7 also)
 * 3) For that matter, what is BRB80 comprised of?
 * 4) What is the pH of the AF cocktail? ( I should look up Glucose oxidase (GOD), Catalase (CAT), and beta-mercaptoethanol (BME))
 * 5) How does Ascetic Acid and AF interact? (it seemed to do terrible things to the tubulin)
 * 6) For that matter, how does AF interact with the MT's?

The Chemicals

 * Glucose Oxidase is an enzyme that binds to beta-D-glucopyranose (a form of the sugar glucose)
 * It is dimeric
 * It uses flavin adenine dinucleotide (FAD), which comes from Redox reactions, to become a catalyst.
 * It also reduces natural 02 into h202 (hydrogen peroxide)

Computer

 * Koch and I got the drivers installed and the computer put on the server
 * Its name is Kochlab-daq2
 * It has 2 ports in the back (as far as I can tell) one for a video card and one for a DAQ card
 * LABView 71 and visual safe source also installed

Video Cards

 * I went to the Dell site and tried to "build my own" optiplex 960
 * I figure if Dell sells the card, they work with the computer. I will also try to price them out on sites through Google shopping
 * Here are the video cards they offer:
 * 256MB ATI RADEON HD 3450 (Dual DVI/ VGA /1 TV-out), full height [add $74]
 * Amazon:~$40
 * 256MB ATI RADEON HD 3470 (Dual DP), full height [add $84]
 * $70-120
 * 512MB ATI RADEON HD4670,Dual DVI/ plus single DVI VGA adapter [add $139]
 * ~$60-70
 * 256MB nVidia GeForce 9300 GE (Dual DVI/ VGA /1 TV-out), full height [add $74]
 * Amazon: 50-60
 * 512MB NVIDIA Quadro NVS 420 Quad DVI Adapter, Full Height [add $499]
 * Amazon: ~480
 * 512MB NVIDIA Quadro NVS 420 Quad DisplayPort Adapter, Full Height [add $499]
 * Amazon: same as above
 * So, any descent card will def be under 100, and we can just ship it in and take 20mins to install it to the computer itself


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