User:Vijay/Notebook/2007-2-17

Targetron for msa knockout

 * Double digested vector, insert (msa and control retargeted)
 * Run gel
 * Gel purify (elute with 40ul for insert, 50ul for vector)
 * Ligate (one hour at room temperature)
 * Transformation in to Dh5, RN4220
 * Plate in amp (Dh5), erm (RN4220)
 * Incubate at 37 (Dh5), 32 (RN4220)