Eccles: DGG Protocols

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Nucleic acid

 * Total RNA isolation with Tri Reagent and columns (Aaron)
 * cDNA synthesis (Jess Protocol)
 * DNA isolation (Jody Protocol)
 * PCR product sequencing (Matt Protocol)
 * qPCR reaction (Jess Protocol)
 * Random oligo hyb (Aaron)
 * Bioanalyzer (Aaron)

Cell culture

 * CO2 for cell culture
 * siRNA transfection (Jess Protocol)
 * siRNA forward transfection with Lipofectamine 2000 (Aaron)
 * siRNA reverse transfection with Lipofectamine 2000 (96-well) or RNAiMAX (24-well) (Aaron)
 * Antisense transfection (Jess Protocol)
 * Culturing NZM cell lines (Jess Protocol)
 * Making primary cells from mouse tissue (Lana)
 * Making primary cells from surgery tissue (Lana)
 * Culturing CB cells (Mouse Embryonic Kidney Cells, SV40 large T positive) (Lana)
 * Collagen coating of cell culture vessels (Lana)
 * Culturing human ADPKD primary kidney cells (Lana)
 * MTT Assay (7d) on CB Cells (Lana)
 * Eccles:MKS Epithelial Cell Culture (Lynn)

Protein

 * Western Blotting (Lana)
 * Western Blot Stripping (Lana)
 * Protein lysates from cells in culture (Lana)
 * Protein lysates from tissue (Lana)
 * MITF western protocol (Lynn)

Genotyping

 * Pax2-1Neu for WAVE dHPLC