Media and solution preparation for this protocol

==  Media and solutions for Arabidopsis transformation == YM Media 1L Mannitol                        10g Yeast extract                 0.4g K2HPO4 (10% w/v stock)            1 ml KH2PO4 (10% w/v stock)             4 ml NaCl (10% w/v stock)                 1 ml MgSO4.7H2O (10% w/v stock)     2 ml • pH 6.8 • Agar 15g/L • Autoclave • When ready to pour add: • Antibiotic selection if required Keep poured plates for 2 days at room temperature to visualise any contamination, then store at 4°C. Minimal A Medium  (Svab, et al, 1975) Liquid 1L Stock solution A  490mL Stock solution C 11mL Milli-Q H2O 499mL Solid 1L Stock solution A  490mL Stock solution B  499mL Stock solution C 11mL • Store 4°C Minimal A Stock Solution A      Final concentration g/490mL   1 x mM K2HPO4  10.5    60 KH2PO4 4.5    33 (NH4)2SO4 1.0    7.6 tri-Sodium citrate.2H20 0.5   1.7 • Autoclave in 490mL aliquots • Store 4°C • Substituting chemicals • K2HPO4.3H2O    13.8g/490mL Minimal A Stock Solution B    Dissolve 15g agar in Milli-Q H2O, make up to 500mL • Autoclave • Store room temperature Minimal A Stock Solution C    Add 1mL 1M MgSO4 to 10mL 40% sucrose • Store room temperature Infiltration media 200ml Final conc. 10X MS salts  20ml  1xMS salts Sucrose  10g  5% MES   0.2g  0.1% Silwet-L77  200