Registry/Measurement kit/Previous versions

The Measurement Kit consists of Promoter Testers, RBS Testers, and an Inverter/Terminator Tester. All Devices are on the 3K3 plasmid. Also, they are being built in both GFP and RFP versions. (GFP versions shown below)

Promoter Tester
The Promoter Tester using GFP is based on  E0240 and using RFP is based on  I2057. Both come in two versions.
 * Version 1 (E/S)
 * ___E X CCDB S___RBS GFP T
 * Cut promoter E/S and insert
 * Can take part back out if necessary


 * Version 2
 * ___E CCDB X___E0240
 * Cut Promoter E/S to insert
 * Forms mixed site so can't remove, real conditions


 * GFP Promoter Tester Version 1 E/S  I20244
 * GFP Promoter Tester Version 2 E/X  I20245
 * RFP Promoter Tester Version 1 E/S  I20246
 * RFP Promoter Tester Version 2 E/X  I20247

RBS Tester
The RBS Tester using GFP is based on  I2055 and using RFP is based on  I2056. Both come in two versions.
 * Versions 1 (E/X)
 * __R0040___E CCDB X____GFP T
 * To insert RBS cut E/S, forms a mixed Spe/Xba site
 * Has too big spacing (full BB prefix) b/t promoter and RBS
 * Easier for testers to use


 * Version 2 (S/X)
 * __R0040___S CCDB X____GFP T
 * Will get 2 spe/xba mixed sites, can't get RBS out
 * Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation
 * Real condition spacing


 * GFP RBS Tester Version 1 E/X  I20248</BBpart>
 * GFP RBS Tester Version 2 S/X  I20249</BBpart>
 * RFP RBS Tester Version 1 E/X  I20250</BBpart>
 * RFP RBS Tester Version 2 S/X  I20251</BBpart>

Inverter/Terminator Tester

 * Based on  T9002</BBpart>

<br style="clear:both" />
 * F2620________E X P1010 S P________E0040
 * Contains a full BB site to allow construction within the device