IGEM:UC Berkeley/2006/TlambdaOlambda

-Start with saturated culture -Wash out all antibiotics 1. pour 1 mL of culture into 1.5 mL eppendorf tube 2. spin (10 sec. at full speed) 3. dump out residue 4. pipette 1 mL of LB  5. resuspend (by vortexing) 6. spin (10 sec. at full speed) 7. dump out residue 8. pipette 1mL of LB  9. resuspend (by vortexing) -setup: 1. pipette 50uL recipient (ie. EC100D) and 50 uL donor into eppendorf 2. mix and incubate (1 hour @ 37C) 3. add 1mL LB  4. mix 5. plate 25 uL on triA or triK --Jenlu 17:34, 26 June 2006 (EDT)