PHYC452Biophysics:Schedule/quiz3

Quiz 3 Topics

This quiz will cover the Beer-Lambert Law, absorbance measurements for protein and nucleic acid concentration measurements, and the laser classes for visible, CW lasers.

Students should know:


 * The equation for the Beer-Lambert law
 * The definition of absorbance and transmittance
 * The units of molar extinction coefficient
 * How to convert from a absorption cross section to molar extinction coefficient
 * The wavelengths used for protein, vs nucleic acids.
 * Absorbance at these wavelengths is primarly from ring structures
 * The three amino acids that contribute most to protein absorbance
 * The power ranges and safety considerations for visible, CW lasers for class I,II,IIa,IIIa,IIIb,IV lasers.
 * See Laser Safety and consider only the old system (not revised).

Hey does any one know the answers to thee points. I don't know where to find them online.
 * 1) The wavelengths used for protein, vs nucleic acids.
 * 2) *280 for protein. 260 for Nucleic Acid
 * 3) Absorbance at these wavelengths is primarly from ring structures
 * 4) The three amino acids that contribute most to protein absorbance
 * they have the ring structure and are phenylalanine, tryptophan, and tyrosine.