SIL071906-Construction of pSB1A2-J23013

[TraGr] PCR JL10/JL11 on Rlambda  (?bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521            (EcoRI/SpeI, Neb2, Large 2062) Product is pSB1A2-J23013 - JL10 Forward EcoRI oligo to bioBrick TraG: CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc JL11 Reverse SpeI oligo to bioBrick TraG: gacttACTAGTgaagcggctcatatcgtgatc