IGEM:IMPERIAL/2007/Notebook/Lab Planning

This page acts as a draft for the aims and projected achievements of the web lab... equipment requests and other information might also be present on the page.

Aims
Week 6:
 * Clone remaining bricks in XL1-Blue
 * BBa_E0240 [GFP]
 * BBa_I13507 [RFP]
 * BBa_T9002 [ptet-luxR-pLux-GFP]
 * BBa_B0034 [RBS]
 * Clone test contructs into BL21(DE3)
 * BBa_I13522 [ptet-GFP]
 * BBa_E7104 [T7-GFP]
 * Midiprep bricks required for ligation
 * BBa_I13522 [ptet-GFP]
 * BBa_R0040 [ptet]
 * BBa_I0500 [pBad]
 * BBa_F2620 [ptet-luxR-plux]
 * BBa_F1610 [luxI]
 * BBa_E7104 [T7-GFP]
 * BBa_S03550 [RBS-araC]
 * BBa_B0015 [Stop]
 * BBa_R0051 [pcI]
 * BBa_E0240 [GFP]
 * BBa_I13507 [RFP]
 * BBa_T9002 [plux-GFP]
 * Ligate constructs
 * Being planned
 * Make cell extracts

Week 7:
 * Clone bricks in XL1-Blue
 * BBa_J23039 [ptet-luxI]
 * BBa_I13504 [GFP]
 * Clone test contructs into BL21(DE3)
 * BBa_B0015 [stop] (as a blank plasmid)
 * BBa_T9002 [plux-GFP]
 * Ligate constructs
 * BBa_R0051 + BBa_I13504 = BBa_I719022 [pcI + GFP]

Outline of week:
 * Monday: Preparation of pre-incubation solution and YT media
 * Tuesday: Growing of BL21(DE3) in YT media
 * Wednesday: Harvesting of BL21(DE3)
 * Thursday: Preparation of cell extracts
 * Friday: Preparation of cell extracts

Cloning-ligation cycle: [Note: With an optimistic view, on cloning-ligation cycle takes 3 days.]
 * Monday: Cloning of plasmids, plating
 * Tuesday: Growing up of colonies for mini/midiprep
 * Wednesday: Mini/midiprep
 * Thursday: (Cloning, plating)
 * Friday: Plasmid testing, (Ligation)

Eqiupment Waiting List
A list of media to be ordered and equipment to be autoclaved

Media
All media has been collected

Autoclave Equipment

 * 10x 250 ml conical flasks
 * 10x 50 ml centrifuge tubes