User:Tara K. Luckau/Notebook/Team ConGen/2011/06/27

{| width="800"
 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
 * style="background-color: #9DB68C" align="center"|  |Main project page
 * style="background-color: #9DB68C" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

Fragment Analysis Submission

 * Submit samples from 23 June 2011 PCR2 (condition indicated by [[Image:20110627 Ex.jpg|15 px]] below)
 * Submit samples from 24 June 2011 ([[Image:20110627 Star.jpg|15 px]] below)
 * [[Image:20110627 FragSubmit.jpg|500 px]]
 * [[Image:20110627 FragSubmitMap.jpg|800 px]]

Evaporation test for Shipping

 * used 22 June 2011 PCR (failed)
 * sealed with flat-top 8-well strip caps, left on Team ConGen computer 23 June - 27 June


 * Plate Weight

Conclusion

 * 0.16 g loss of liquid via evaporation after 4 days at room temperature
 * $$ 0.16 g \bullet \tfrac{1 mL}{1 g} \bullet \tfrac{1000 \mu L}{1 mL} = 160 \mu L $$
 * 32 rxn * 10µL - 40µL gel = 280µL sample in the plate; so >1/2 liquid is lost!
 * BUT: there's still at least ~ 3µL left in all wells, which is plenty for fragment analysis
 * BUT: when shipping, plate will be cold, and will only take 2 days
 * thus, sealing this way is a-okay!


 * }