BISC 219/F10: Assignment Series2 Complementation

Lab 5 Complementation Assignment
Complementation analysis is an important and widely used genetic tool. We will use it to confirm and expand our knowledge about the gene mutation that is responsible for the phenotype abberation you are studying. You should find and read some general background information before you start writing this assignment. A good place to start is the Wikipedia article found at:. An excellent scholarly article by Hawley and Gilliland can be found in Genetics, Vol. 174, 5-15, September 2006, at doi:10.1534/genetics.106.064550. Your homework assignment to show that you understand complementation and how you will use it in our Series 2 forward genetics project will be due at the beginning of lab next time.

Please write a 1-2 page summary describing complementation analysis as a genetic tool both generally and specifically related to your project. You will probably need to use and cite outside sources (NOT Wikipedia!). Please use the journal Genetics as a model for in text and reference page cition style. You must paraphrase information from sources (no direct quoting in any work submitted for BISC219) and you must not cite Wikipedia or the lab wiki since they are secondary sources and not peer-reviewed. Use Wikipedia initially to learn more about your subject and to find appropriate primary sources to cite. Since you are using sources and citing them in-text, an appropriately formatted Literature Cited page is required as part of this assignment. Include diagrams of the crosses you will set up for complementation analysis from beginning to end including expected results when your mutation is allelic and non-allelic to a reference mutation. Use templates provided.

Grading Rubric
Assignment Complementation Analysis 5 points