IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/06

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Entry title

 * Measured the flourescence of all the inoculated broths from the previous day.
 * I13521; J04450; were good. Showed red Flourescence.
 * 502(B); 500(B); 019(C); 019(B); I13600; I13602; did not show cyan flourescence > these cupped.
 * J+S(B)--> might have cupped.
 * no minipreps done as RFP parts were already there and CFP cupped.
 * Ligations: (F set)
 * J23119 + 1C3;
 * S03879 + 1C3;
 * K17005 + 1C3;
 * K145151 + 1C3;
 * J13002(1) + 1C3;
 * J13002(2) +1C3;
 * B0014 + 1C3.
 * Also J23119(S/P)(A) + E0420(X/P)(C) --> selected on amp.
 * R0010(S/P)(C) + E0420(X/P)(A)(RK) --> select on Chl.
 * J23119(S/P)(A) + I13507(X/P)(C) --> select on Amp.
 * R0040(S/P)(C) + E0420(X/P)(A)(RK) --> select on Chl.
 * Transformed I13522; I13504, E0430, from the Kit and E0420 from RK.
 * Ran the Gel for all the digest.
 * Gel Order.
 * B0014(E/P); E0420(X/P); E0420(X/P)(RK); J13002(E/P)(1);J13002(E/P)(2); I13507(X/P); J23119(E/P); Ladder; J23119(S/P); K117005(E/P); K145151(E/P); R0010(S/P);R0040(S/P); S03879(E/P); 1C3(E/P).


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