ISISBio:Internal/Group meeting/20-June-08

Visitor from Kings for next week


 * plan to transform two plasmids, this afternoon into Top10, Bl21(DE3), KRX
 * out of incubator tomorrow morning ready for overnight cultures on Monday
 * grow Tuesday and overnight for KRX
 * start purification on Wednesday
 * keep things simple for Thursday
 * gel filtration Friday

Michael


 * have two colonies - might be the right thing
 * recloning of sortase - redoing because got no colonies
 * hope to be growing up next week

Vicky G-S


 * still haven't confirmed whether made the right thing or not
 * waiting on enzyme to test that
 * amplified up all other fusion partners

Mark Telling


 * Will try to schedule GFP experiments for September or later