User:Karmella Haynes/Notebook/Polycomb project/2010/05/05

{| width="800"
 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Project name
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

05/05/10

 * &#x2713; FlpE: cultures (2 from 20 μg/mL Amp plate, 2 from 100 μg/mL Amp plate)
 * &#x2713; RT-PCR: Pc-ATF 4-day dox set, p14ARF & KRT17
 * &#x2713; Senescence assay cultures: Expand 132-4, -6 to 10 cm plates; KAH126-1 not growing well (freeze down current culture, PS7, on next passage); thaw fresh U2OS Flp-in T-REx

RT-PCR

> Continued from 5/04/10: semi-qRT-PCR --> cDNA templates
 * 1) KAH126-1
 * 2) KAH126-1(+dox)
 * 3) KAH127-4
 * 4) KAH127-4(+dox)
 * 5) KAH128-8
 * 6) KAH128-8(+dox)
 * 7) KAH129-4
 * 8) KAH129-4(+dox)
 * 9) KAH130-2
 * 10) KAH130-2(+dox)
 * 11) KAH131-9
 * 12) KAH131-9(+dox)
 * 13) KAH132-8
 * 14) KAH132-8(+dox)
 * 15) KAH133-1
 * 16) KAH133-1(+dox)
 * 17) FTRx
 * 18) FTRx(+dox)

--> Primers; cDNA dilution
 * 1) p14ARF(6); 1:1
 * 2) KRT17(14A); 1:100

--> Quantitate KRT17 using Image J


 * }