Jacobs:Protocol Pipetting Exercise

Materials

 * Pipetman (20ul, 100ul, 1000ul)
 * Pipet tips
 * 1.5 ml microcentrifuge tubes
 * Beaker with distilled water
 * Analytical balance
 * Laboratory marking pen

Procedure

 * 1) Label 8 microcentrifuge tube lids separately: 2 with “1ul”, 2 with “20ul”, 2 with “100ul” and 2 with “500ul”
 * 2) Weight each tube on the analytical balance and record the mass to the nearest 0.001g
 * 3) Pipet the volume of water written on each tube into the tube
 * 4) Re-weigh the tubes and record the mass to the nearest 0.001g
 * 5) Calculate the mass (g) of the water in each tube (Mass of tube with water (g) – Mass of tube without water (g) = Mass of water (g))
 * 6) Check accuracy of pipetting technique knowing that 1ml water = 1gram
 * 7) e.g., 200ul water = 0.2ml = 0.2g

Contact

 * History: CRJ-ABC, last updated: 8/3/07

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