Barry Canton/Bacterial lysis

I need to lyse some E. coli under denaturing conditions. I'm collecting my on how to best do that here.

OWW protocols

 * 1) Sauer:Purification of His-tagged proteins/Denaturing prep - the lysis part of this should generally useful
 * 2) Knight:Purification of His-tagged proteins/Denaturing - slightly different lysis buffer
 * 3) RNA extraction - describes use of guanidine isothiocyanate
 * 4) Sauer:ClpX purification/Protocol 1
 * 5) Sauer:His6 ClpX purification
 * 6) Sauer:SspB purification - Tween
 * 7) Sauer:Lysing E. coli with Lysozymes - lysozyme methods for native purification
 * 8) Sauer:Expression/purification of 35S-Met proteins/Native purification - Imidazole
 * 9) WangLab:Lysis Buffer

Lysing reagents

 * 1) NaH2PO4, Guanidine hydrochloride, imidazole
 * 2) Urea, TCEP, imidazole, NaH2PO4
 * 3) Lysozyme and guanidine isothiocyanate
 * DTT, PMSF
 * 1) NaH2PO4, imidazole, DTT
 * 2) beta-ME, Tween-20, PMSF
 * 3) NaH2PO4, Imidazole
 * 4) Triton, SDS, NAF

Chemical functions

 * 1) Guanidine hydrochloride - denatures protein
 * 2) NaH2PO4 - pH buffer
 * 3) Imidazole - used for his-tagged protein purification
 * 4) Urea - denatures protein
 * 5) TCEP - breaks disulfide bonds
 * 6) Guanidine isothiocyanate - denatures protein
 * 7) DTT - breaks disulfide bond
 * 8) beta-ME - breaks disulfide bonds
 * 9) Tween - detergent
 * 10) PMSF - serine protease inhibitor
 * 11) Triton - nonionic detergent
 * 12) SDS - detergent
 * 13) NAF -