User:Mbennie/Notebook/Sequencing/8.6.2007

Template: 8ul DNA, .3ul primer, 1.7 ul water

NNNNNNNNNNNTNNNTATNNNAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGA TGATTTCTGGAATTCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGTGACGTGGTGCGCCCGCGGTCAAACACTT CGCACCATTCGGCCTGTTTATCCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCG AAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATC AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAAT CGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCG CTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGAC CGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA GAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAA ACCACCGCTGGTAGCGGNGNNNTTTTTGTTTGCAGCAGCNNATTACGCGCANAAAAANNGNTCTCAGANATCCTTTGNTC TTTNTANNNNCTGACNCTCANTGNNNNAAACTCACGTTAANNNNTTNGNCATNANNTNTCAAANGATCNTNNCNAGATCT TTNNNNNNNAANNNNTTTTNAATNANNNAANNNNNNNNNNNNCTGNNNNNNNNNNNNNCTNNANNCNCNCNNNAANNNNN NGGNNCNNNNGNNNNNNANNAANNNNNGGNNNNNNNNGNNNNNNNNNNNGGANNNTNNNACNNNGNNNNNNN
 * IgAss2 with VF2
 * No significant similarity

NNNNNNNNNNNTNNNNATANAAATAGGCGTATTACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGA TGATTTCTGGAATTCGCGGCCGCTTCTAGATGAAAGCCAAACGTTTTAAAATTAACGCCATATCCTTATCCATCTTTCTT GCCTATGCCCTTACGCCATACTCAGAAGCGGGTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACC ACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGG TCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGA AAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCG CCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCG GGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGT GCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGNGCTACAGAGTTCTTGAAGTGG TGGCCTAACTACNGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCNGAAAAGAGTT GGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGNNGNTTTTTGNTTGCAGCAGCNNATTACGCGCANAAAAA GNANCTCAGANATCCTTGATCTTTCTANGGGTCTGANNCTCANNGNANNAANNTCANGTNAANGATTNTNGNCANNANAN TNNTCNANNANGNNNNTTNNCNANANCTNNANNNNNANGNANNNNANCAANCNNNNNNNNNNNNNAACNNNNNGNNANNN NNNNNNNNNNNNNNNNNNNAAANNNCNNNNNNNNCNNNNNTNNTNNAANANNNNNNNNNNNGNNN
 * IgAss3 with VF2
 * 100% correct

Missing part 2 NNNNNNNNNNANNTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGA TTTCTGGAATTCGCGGCCGCTTCTAGAGGTAGCACGCAATCTATCTTGTTGGATACAGGTGCTTCATTAACACGGCACAT TGCACAAAAATCACGCGCTGATGCCGAAAAAAACAGTGTTTGGATGTCAAACACCGGTTATGGCCGTGATTATGCTTCCG CACAATATCGCCGGTTTAGTTCGAAACGCACGCAAACACAAATCGGCATTGACCGCAGCTTGTCCGAAAATATGCAGATA GGCGGAGTATTGACTTACTCTGACAGTCAGCATACTTTTGATCAGGCGGGCGGCAAAAATACTTTTGTGCAAGCCAACCT TTATGGTAAGTATTATTTAAATGATGCTTGGTATGTGGCCGGCGATATTGGTGCGGGCAGCTTGAGAAGCCGGTTACAAA CGCAGCAAAAAGCAAACTTTAACCGAACAAGCATCCAAACCGGCCTTACTTTGGGCAATACGCTGAAAATCAATCAATTC GAGATTGTCCCTAGTGCGGGTATCCGTTACAGCCGCCTGTCATCTGCCTGAAGAGCTCTTCAGCCGCTTACTTTGCCAAT TATGGCAAAGGCGGCGTGAATGTGGGCGGTAAATCCTTCGCCTATAAAGCAGATAATCAACAGCAATATTCAGCAGGCGC CGCGTTACTGTACCGTAATGTTACATTAAACGTAAATGGCAGTATTACAAAAGGAAAACAATTGGAAAAACAAAAATCCG GACAAATTAAAATACAGATTCGTTTCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAGGGCAAGGTGTCACCA CCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCANGCTTCCTCGCTCACTGACTCGCTGCGCTCGGT CGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTANNACGGTNATCNCAGAATCNGGGGANACGNNGAAAGA ACNTGNNAGCAAAGNCAGCNAAAGNCNGNANCGTAAAANGCNNCGTTGCTGGNGTTTTNCACNGNNCCGNCCCCCNGANN ANNNTNANNAAAATCGANNNTCAAGNCNNNGGNGNNNAANCNNNNNNNNNNNTNAANANNNNCNGNNNNNTCNNNNNNNN NNNNNNNNCNNNNNNNNNNNNNNNNNNNNACCNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNTNNNNTNNNNNGNNNNNN NNNGNNNTTTNNNNTANNNNNNNNNNNNNNN
 * IgAbc1-F with VF2

Missing part 2 NNNNNNNNCNNANNNACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTT TAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGAAACGA ATCTGTATTTTAATTTGTCCGGATTTTTGTTTTTCCAATTGTTTTCCTTTTGTAATACTGCCATTTACGTTTAATGTAAC ATTACGGTACAGTAACGCGGCGCCTGCTGAATATTGCTGTTGATTATCTGCTTTATAGGCGAAGGATTTACCGCCCACAT TCACGCCGCCTTTGCCATAATTGGCAAAGTAAGCGGCTGAAGAGCTCTTCAGGCAGATGACAGGCGGCTGTAACGGATAC CCGCACTAGGGACAATCTCGAATTGATTGATTTTCAGCGTATTGCCCAAAGTAAGGCCGGTTTGGATGCTTGTTCGGTTA AAGTTTGCTTTTTGCTGCGTTTGTAACCGGCTTCTCAAGCTGCCCGCACCAATATCGCCGGCCACATACCAAGCATCATT TAAATAATACTTACCATAAAGGTTGGCTTGCACAAAAGTATTTTTGCCGCCCGCCTGATCAAAAGTATGCTGACTGTCAG AGTAAGTCAATACTCCGCCTATCTGCATATTTTCGGACAAGCTGCGGTCAATGCCGATTTGTGTTTGCGTGCGTTTCGAA CTAAACCGGCGATATTGTGCGGAAGCATAATCACGGCCATAACCGGTGTTTGACATCCAAACACTGTTTTTTTCGGCATC AGCGCGTGATTTTTGTGCAATGTGCCGTGTTAATGAAGCACCTGTATCCAACAAGATAGATTGCGTGCTACCTCTAGAAG CGGCCGCGAATTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTAT TTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGNNNTTTTNNGGGAAATGNGCGCNGAANCNCT ANTTNNTNNTTTTNCTAAATNNNTNNAANANGNNNCCGNTCNNGANNNANTANCNGATAATGCTCANNNNNNTNNNAANG NANANNNNGANNTNANNNTNCNNNNNNCCNTNNNNNTTTNNNNNNNTTNNNNNNTNNNNNNCCNNNACNNNGGGNAANNN NANNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNATNN
 * IgAbc1-R with VR

Sequencing problem NNNNNNNNNNNTTACCTATANNATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGA TGATTTCTGGAATTCGCGGCCGCTTCTAGAGGTACTGATACACTCCAAGCGGAGACAGACCAACTAGAAGATGAGAAGTC TGCTTTGCAGACCGAGATTGCCAACCTGCTGAAGGAGAAGGAAAAACTACAGTTCCTCCTGGCGCCTCATATAGGNNCTC GNNNCTACCNCCCNCTTCAGCATGANATGANCNGTCANTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTNANNNTTT TANCTTCACTCTNGGGCTTTCTTCNTTTTCNTCACAGTTNNNNTGNNTCNGNGGGGACATTTGGCTGCTGCGANNNATAC CATNTNACTTGGANNCCGTTNANCATGTATCTAN
 * Fos1 with VF2

No significant similarity NNNNNNNNTNACCNNTNANAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATG ATTTCTGGAATTCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGTGACGTGGTGCGCCCGCGGTCAAACACTTCG CACCATTCGGCCTGTTTATCCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAA AAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAG CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAA AAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCA CGCTGTAGGTATCTCATTTCGGGTGTAGGTCGTTCGCTCCAGCTGGGGCTGTGTGCACAACCCCCCCGTTCAGNCCNACC GCTGCTNCCTTATCCGGNAANTATCANNTGAATCCAACNN
 * JunB3 with VF2

No significant similarity NNNNNNNNNNNTNNNNTATNNNNATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGGACTCGGCATGAACATGTTCGAACAGCAGCAGTGACGTGGTGCGCCCGCGGTCAAACACT TCGCACCATTCGGCCTGTTTATCCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACC GAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTAT CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAG CAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAA TCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGC TCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGA CCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGNAAGACACGACTTATCGCCACTGGCAGCAGCCACTG GNNACAGGATTAGCAGANCNAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGTNGGCCCTAACTNCNGCTACNCTAG ANAACANNATTGNTATCTGCGCTCCTGCTGANNCNNNTACCTTTNNNAAAAATANTTGNNNNCTCCTGNTCCGNNAANNN
 * GCN42 with VF2