User:Howard Boland/Notebook/Art from Synthetic Biology/2010/11/06

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Transformation Result
From the two transformation set up yesterday I observed one colonies one of my plates

Here it is! I actually only discovered this a couple of days later

Gel Result PCR, expected 2.3kb
I ran out my PCR from Thursday night on a 1% gel to verify and cut out the expected 2.3kb band


 * 1) Lane 1: 10µl 1kb NEB Quick Ladder
 * 2) Lane 2: 50µl pcr product, 2300bp, pSense66, cut
 * 3) Lane 3: BLANK
 * 4) Lane 4: 50µl pcr product, 2300bp, pSense66, cut
 * 5) Lane 5: BLANK
 * 6) Lane 6: BLANK
 * 7) Lane 7: 2µl Template, pUA66kate 25-10-10-NA
 * 8) Lane 8: Blank




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