IGEM:British Columbia/2009/Protocols/Glycerol Stocks

Supplies Needed:

 * 60% autoclaved glycerol
 * Day/overnight culture
 * 1.5mL microfuge tube/cryovial

Steps:

 * 1) Label the tube with: strain, date, BioBrick part number/plasmid or other identifying information, antibiotic (if necessary)
 * 2) Aseptically add 0.25mL 60% glycerol to the tube, then add 0.75mL of culture (final glycerol concentration 15%).
 * 3) Vortex briefly.
 * 4) Put in -80°C freezer.