Maheshri:Internal

=General Information=


 * Guidelines for New Members
 * Lab Duties
 * Standard Operating Procedures for the Equipment
 * Common Area Etiquette
 * Locations of Common Items and Reagents
 * Enzymes
 * Abbreviations
 * Equipment
 * Computing

=Protocols=
 * Media
 * Buffers
 * Culturing Yeast
 * Non-selective Mating of Yeast
 * Sporulation in Liquid Media
 * PCR
 * Kinase and Anneal Oligos
 * Restriction Digestion
 * Agarose gel electrophoresis
 * Gel Extraction
 * DNA Ligation (OpenWetWare)
 * Making Chemically Competent Bacteria
 * Making Electro-competent Bacteria
 * Bacterial Transformation
 * Yeast Transformation
 * Bacterial Plasmid Miniprep
 * Yeast Transformation by Electroporation
 * Yeast Genomic Prep
 * Yeast Plasmid Prep
 * Ethanol Precipitation of Nucleic Acids
 * Yeast Protein Prep
 * SDS-PAGE
 * Western Blotting
 * mRNA FISH
 * Micrococcal Nuclease Assay
 * RT-PCR
 * Fluorescence Microscopy

=Software=
 * MATLAB
 * VectorNTI; speak to the Computer Guru about obtaining a copy. VectorNTI tutorials & reference guide

=Database=

=Useful Links=
 * Saccharomyces Genome Database (SGD)
 * NCBI Homepage
 * Codon Usage
 * Biological Sequence Alignment
 * Current Protocols in Molecular Biology
 * Getting Started with Yeast
 * NEB Double Digest Finder
 * Nikon MicroscopyU