IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/12/Shan Shen

Prepare competent cells. Construction

8:00 The order of the samples: marker, plasmid control of 1-18A, digestion product of 1-18A The digestion products run faster than the plasmids.

9:30 Shake 1-18A and 1-18C again.

15:30 Electrophoresis to test the digestion products The order and the amount of the samples: marker 5μL, plasmids of high-copy bi-stable system 3μL, digestion products of hi-copy bi-stable system 20μL, plasmids of low-copy bi-stable system 5μL, digestion products of low-copy bi-stable system 20μL. Results: Bands are smeared.

16:20 Digest high-copy bi-stable system and low-copy bi-stable system again.

22:00 Electrophoresis to test the digestion products The order and the amount of the samples: marker 5μL, plasmids of high-copy bi-stable system 3μL, digestion products of hi-copy bi-stable system 20μL digestion products of low-copy bi-stable system 20μL. Results: High- copy plasmids have been successfully digested, while the low-copy plasmids are still digested to be smeared.

22:30 Miniprep of 1-18A and 1-18C

23:00 Digest 1-18A.