User:Elad Noor/Notebook/Growth rates of Baceria/2008/08/17

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Plan
The script used was put in D:/Script/Elad/RowGradients_3fold.conf Preparations:  Put 100 mL of oil in TR2 Put 150 mL of M9 in TR3 Put the test-tube with the bacteria (MG1655), grown overnight on 4 mL of M9 + glucose 0.2% at T1 - row 1 Put 1 mL of each sugar (3% concentration) in a test-tube in T2 rows 1-6, I diluted 0.15 mL of the sugar at 20% with 0.85 mL of M9 Fill the tips Put 6 plates at P1-6 Put one deep-well plate at S1</li> </ol>

The order of sugars is: <ol> D-glucose</li> D-arabinose</li> glycerol</li> succinate</li> D-mannose</li> D-ribose</li> </ol>

Problems with the script: <ul> DILUTE_SERIAL doesn't distribute the medium to all wells with one swipe, but picks up 135 uL of M9 once for every column.</li> It also doesn't remove a third of the liquid from the last column so there is 50% more liquid than in the other columns. The concentration there will be wrong. ignore it for all plates.</li> Plate P3 (glycerol) had a grave problem with the medium distribution. Ignore the results in it.</li> </ul>


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