User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/05/12

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Maize Endophyte Biofertilizers
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The Daily Grind

 * Sarah made electrocompetent seed endophytes (about 15) and electroporated them with pDSK-GFPuv as per the E. coli instructions. 1mm cuvettes and program Ec1 on the Biorad electroporator.
 * Transformed ligations of partially NdeI cut alSD operon and NdeI cut ACC deaminase into (dephosphorylated) NdeI cut pSBA12 with PSBA::GFPuv and NdeI cut pDSK-GFPuv


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