SDS sample buffer

Purpose

 * For preparation and loading of protein samples onto a gel for SDS-PAGE analysis.

Preparation
To make 10 mL of 4X stock:
 * 2.0 ml 1M Tris-HCl pH 6.8
 * 0.8 g SDS
 * 4.0 ml 100% glycerol
 * 0.4 ml 14.7 M &beta;-mercaptoethanol
 * 1.0 ml 0.5 M EDTA
 * 8 mg bromophenol Blue

Final concentrations (1X):
 * 50 mM Tris-HCl pH 6.8
 * 2% SDS
 * 10% glycerol
 * 1% &beta;-mercaptoethanol
 * 12.5 mM EDTA
 * 0.02 % bromophenol blue

Use
Dilute protein sample 1:3 into 4X sample loading buffer. Load on acrylimide gel in SDS-PAGE buffer.

Safety
&beta;-mercaptoethanol is a severe irritant and is readily absorbed through the skin. SDS is a respiratory irritant in solid form and a mask should be worn while weighing it.
 * &beta;-mercaptoethanol MSDS