User:Mbennie/Notebook/Papers and Books/Outer Membrane Proteins Overviews


 * 1) OmpX pmid=16600968
 * Fused the N and C terminals of the OmpX protein and created a protein with a N and C terminus that are outside the cell (by 'cutting' one of the beta-barrel loops that was outside the cell)
 * About 500 bp
 * Other systems that might have external display of C or N terminal: INP, EaeA intimin, and EstA
 * Can be overexpressed without damage to membrane
 * Less efficient than OmpX
 * MIT 2007 IGEM team is working with this protein - check back in the fall and hopefully it will already be a part


 * 1) Protein-Display-Review pmid=12039531
 * Thorough review of all significant surface display proteins
 * Surface proteins facilitate diverse functions such as "cell-cell recognition, signal transduction, surface adherence, colonization, and immunoreactions"
 * Table 1 (p. 131) contains good overview of surface display proteins sorted by family
 * Promising system: Lpp'OmpA (p. 133) carries large passenger and expresses on cell surface, folding of passenger can be a problem
 * Lots of system with various drawbacks: not well studied, small passenger, and structure
 * Section on Gram positive bacteria and applications


 * 1) Microbial-Surface-Display-Review pmid=12480350
 * Four requirements for a carrier protein (all quoted)
 * Efficient signal peptide or transporting signal to allow premature fusion protein to go through the inner membrane
 * Strong anchoring structure to keep fusion proteins on the cell surface
 * Compatible with foreign protein
 * Resistant to proteases in the the periplasmic space or medium
 * Describes several system of surface display including sections on Gram positive bacteria and yeast


 * 1) Voulhoux-Role pmid=12522254
 * Omp85 plays a crucial role in the functionality of IgA and other outer membrane proteins
 * Inner membrane - phospholipid bilayer, outer membrane - asymmetric with phospholipids and lipopolysaccharides
 * Signal sequence is cleaved off after translocation into the periplasm
 * omp85 is essential for growth
 * "In the Omp85-depleted strain, the processed passenger domain of IgA1 protease was barely detectable, and the full-length form was found to accumulate"


 * 1) Benz-AIDA-1 pmid=1625582
 * Discovery of adhesin involved in diffuse adherence (AIDA) in e coli
 * Has 49 aa signal sequence
 * 50% homology to virG in S. flexneri