User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/27

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12/27/09

 * Re-transform: KAH93/V0120; JDS42, 43
 * Assemblies: KAH136; KAH137, 138 (do-over)

Assemblies
 * 1) KAH136: (1) PEST/(S/P)/3734 + EED/(X/P)/1401 &#x2713;
 * 2) KAH137: KAH78/(E/S)/3652 &#x2713; + KAH134/(E/X)/4600 &#x2713;
 * 3) KAH138: KAH79/(E/S)/3652 &#x2713; + "

> Digests (Fermentas FD)

> Measure conc.'s

> Ligations

--> For KAH137 & 138, use less vector and 3:1 insert to minimize background


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