IGEM:The Citadel/Notebook/Notes Brian/07-27-10

Biobrick construction map:

0.1 J23102 (A)[E,P] + pSB1C3 (PCR)[E,P] = J23102 (C, ex A) -- COMPLETED! (Tube A) 0.2 B0015 (A)[E,P] + pSB1C3 (PCR)[E,P] = B0015 (C, ex A) -- COMPLETED! (Tube B) 0.3 C0160 (A)[E,P] + pSB1C3 (PCR)[E,P] = C0160 (C, ex A) -- COMPLETED! (Tube C) 0.4 J23102 (C, ex A)[E,S] + E0240 (A)[X,P] + pSB1T3 [E,P] = ~J02E40 [3A Assembly Test] 1.0 J23102 (C, ex A)[E,S] + F1610 (A)[X,P] + pSB1T3 [E,P] = ~J02F10 (T) 2.0 I746350 (A) + B0015 (C, ex A) + pSB1T3 = ~I50B15 (T) 2.1 ~I50B15 (T) + I746360 (A) + pSB1C3 = K274370 (C) 2.2 ~I50B15 (T) + I746361 (A) + pSB1C3 = K274371 (C) 2.3 ~I50B15 (T) + I746364 (A) + pSB1C3 = K274374 (C) 2.4 ~I50B15 (T) + I746365 (A) + pSB1C3 = K274375 (C) 3.0 I746351 (A) + B0015 (C, ex A) + pSB1T3 = ~I51B15 (T) 3.1 ~I51B15 (T) + I746360 (A) + pSB1C3 = K274380 (C) 3.2 ~I51B15 (T) + I746361 (A) + pSB1C3 = K274381 (C) 3.3 ~I51B15 (T) + I746362 (A) + pSB1C3 = K274382 (C) 3.4 ~I51B15 (T) + I746364 (A) + pSB1C3 = K274384 (C) 4.0 I746352 (A) + B0015 (C, ex A) + pSB1T3 = ~I52B15 (T) 4.1 ~I52B15 (T) + I746361 (A) + pSB1C3 = K274391 (C) 4.2 ~I52B15 (T) + I746362 (A) + pSB1C3 = K274392 (C) 4.3 ~I52B15 (T) + I746364 (A) + pSB1C3 = K274394 (C) 4.4 ~I52B15 (T) + I746365 (A) + pSB1C3 = K274395 (C) 5.0 F2620 (A) + K274382 (C) + pSB1T3 = ~F20K82 (T) 5.1 ~F20K82 (T) + E0240 (A) + pSB1C3 = ~F20K82E40 (C) 5.2 ~J02F10 (T) + ~F20K82E40 (C) + pSB1A3 = ~J02F10F20K82E40 (A) [QS & ST tester] 6.0 B0034 (A) + C0160 (C, ex A) + pSB1T3 = ~B34C60 (T) 7.0 ~B34C60 (T) + B0015 (C, ex A) + pSB1A3 = K218026 (A) 8.0 ~J02F10 (T) + F2620 (A) + pSB1C3 = ~J02F10F20 (C) 8.1 ~J02F10F20 (C) + E0240 (A) + pSB1T3 = ~J02F10F20E40 (T) [QS Test]
 * J23102 to be cut with E & P and inserted into pSB1C3 prior to assembly.
 * B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.
 * B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.
 * B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.