Jessica Karen Wong/Notebook/2007-7-23


 * Minipreped I2055 for seq w/ VF and VR
 * Got some sequencing redo's back and I2055-3K3 which didn't work, nothing incredibly enlightening


 * Did a scarring/promoter insert PCR of I2056 on a gradient (50-56) w/ supermix
 * Ran a gel, all lanes looked good
 * Doing a 100ul PCR of I2056 at 51 overnight


 * Wrote up proposal for synthesis of I2055


 * Ordered new primers
 * CTTAGTAG GCGGCCGC ACTAGT G TCACACAGGAAAGTACTAGATGCG, BBbp_E0240_Fwd_Spe
 * CTTAGTAG GCGGCCGC TCTAGA G TCACACAGGAAAGTACTAGATGCG, BBbp_E0240_Fwd_Xba
 * CTTAGTAG GCGGCCGC ACTAGT G TCACACAGGAAAGTACTAGATGGC, BBbp_I2057_Fwd_Spe
 * CTTAGTAG GCGGCCGC TCTAGA G TCACACAGGAAAGTACTAGATGGC, BBbp_I2057_Fwd_Xba
 * TCAGCGAT GCGGCCGC ACTAGT A GTGCTCAGTATCTCTATCACTGATAGG, BBbp_R_Rev_Spe
 * CTTAGTAG GCGGCCGC TCTAG ATGCGTAAAGGAGAAGAACTTTTC, BBbp_I2055_Fwd_Xba
 * CTTAGTAG GCGGCCGC TCTAG ATGGCTTCCTCCGAAGACG, BBbp_I2056_Fwd_Xba