User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/09

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Yeast Culture Prep for Long Term Freezer Storage
From Revived Yeast Culture NCYC1001

1) Overnight culture on agar plate

Streaked out 50.0ul of growing culture onto a glucose+yeast extract plate

S. pombe - failed to grow - redo S. cerevisiae - good lawn

2) Prepare freezing media = 20% gycerol in sterile YM media. Aliquot into small freezer tube (1000ul).

3) Collect lawn with sterile toothpick and swirl in freezing media

4) Slow freeze in -30C

Addition of emPCR-Compatible Sites and Library Enrichment
load 3.0 ul of sample