User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/12

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 * style="background-color: #EEE"|[[Image:OpenWetWare_photo.jpg‎|128px]] Alien genes
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Ethanol Post-PCR Clean Up
Procedure

1) For each 10 ul of PCR product, prepare the following mixture in strip-cap tube. 1 ul of 3M sodium acetate (NaOAc) pH 5.2 25 ul chilled absolute ethanol. 2) Transfer the PCR product (20ul) into the tube containing the mixture. 3) Vortex the tube and then store it at -20 for 30-50 min. 4) Spin for 30 min at maximum speed 14.000 rpm. 5) Rremove the supernatant carefully and don't disturb the pellet. 6) Wash the pellet with 70% ethanol. And spin the tube at maximum speed for 5 min. 7) Remove the supernatant and then dry the pellet – air dry. 8) Resuspend the pellet in 10 ul in sterile water.