Huang: Amino Silane Treatment of Glass Slides

Overview
This protocol allows the immobilization of bacterial cell on glassware and improves the following imaging. We use amino silane instead of poly-lysine to achieve robust graft.

Materials

 * 1 ml amino silane (we use (3-Aminopropyl)triethoxysilane from Sigma-Aldrich)
 * 100 ml acetone
 * 5 Glass microscope slides (or cover slips)
 * Staining Jar

Procedure

 * 1) Mix 1 ml silane with 50 ml acetone in the staining jar (2% silane).
 * 2) Clean the glass slides or cover slip. To achieve this, you can either
 * 3) rinse the glass with ethanol and wipe with Kimwipe, and then blow dry. Or
 * 4) ultrasonic cleaning with 10% micro90 detergent for 30 min, rinse with water and then blow dry. Or
 * 5) plasma cleaning. Or
 * 6) follow the protocol in Pet Brown's lab at Stanford
 * 7) Immerse the glass in the jar for 20 min.
 * 8) Rinse the glass in a conical tube with 50 ml acetone.
 * 9) Blow dry.
 * 10) Bake at 120°C for 20 min (optional).

Contact

 * Jianbin Wang