Berglund:Kristy Method-U2AF65

Materials
10X Binding Buffer C (BBC) 250mM Tris pH 7.5 500mM NaCl 10mM DTT 10mM EDTA 10X Binding Buffer D (BBD) 500 mM Tris pH 7.5 50 mM MgCl2 1 M NaCl 0.1% Triton X-100 10mM DTT 10X native dye 200µL 5X TBE or TB 400µL 100% glycerol 250µL 2% bromophenol blue 150µL H2O recombinant purified U2AF65 kinased RNA of your choice