User:Anthony Salvagno/Notebook/Research/2011/03/04/Prepping Unzipping With pALS

PCR
I cleaned up the PCR reaction from Thursday and the nanodrop revealed:
 * 227ng/ul --> 85nM in 30ul (total of 6.8ug of DNA)

Adapter Annealing
Since I got a new bottom oligo with 5' biotinylation I will have to anneal a new adapter duplex.

Ligation
I only have SappBR so I will need to ligate the adapter to here first and then ligate this to the digested pALS.

SappBR-Adapter Ligation
After purification:
 * ApBR:
 * 89ng/ul --> ~31nM -- ~2.7ug
 * DpALS:
 * 151.1ng/ul --> ~57nM -- ~4.5ng