Chang Lab:Notebook/CBE/08/149/2008/12/05

{| width="800"
 * style="background-color: #FFF"|   Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page
 * style="background-color: #F2F2F2" align="center"|  |Main Notebook page


 * colspan="2"|
 * colspan="2"|

Logs

 * Started at 0945am.
 * Culture medium
 * 1g of BD Agar and 11g of BD Broth weighed out on a weighing scale into a 1000ml flask.
 * Add 200ml of distilled water
 * Add 400ml of BD Agar solution (14g BD Agar to 400ml distilled water) and 400ml of BD Broth (14g LB Broth to 400ml distilled water).
 * Stir well and separate into 3 bottles (300ml each).
 * Autoclaved 1030am at 121 degrees for 15min (waited for about 2hrs for the temperature to rise and pressure to build up and lower). Temperature safe to remove is 80 degrees.
 * Prepared E coli (K12 W3110) broth mixture and incubated at 30 degrees.
 * E coli was filled with LB Broth in a test tube (ratio 0.1ml:9ml).
 * Placed in a shaking incubator at 252rpm for 6hr at 35.2 degrees (incubated from 10am - 5 pm).
 * Culture streak
 * Streaked 2 LB agar plates using E coli broth culture for overnight incubation at 37.0 C.


 * }