Mazel/pSW8197

LOCUS      pSW8197        3490 bp    DEFINITION  Ligation of pMP7 = pSW23T-ccdB-xbaI-sacI* into pSW29T* ACCESSION  alternate name pMP15 KEYWORDS. SOURCE     Unknown. ORGANISM Unknown Unclassified. REFERENCE  1  (bases 1 to 3490) AUTHORS  Marie-Eve VAL JOURNAL  Notebook COMMENT    SECID/File created by Clone Manager, Scientific & Educational Software COMMENT    SECNOTES|Author:: Marie-Eve VAL Date:: 02 Mar 2009 Notebook:: Vector molecule: pSW29T* Fragment ends: SacI and XbaI Fragment size: 1930 Insert molecule: pMP7 = pSW23T-ccdB-xbaI-sacI* Fragment ends: XbaI and SacI Fragment size: 1560 FEATURES            Location/Qualifiers misc_feature   46..302 /gene="oriT-RP4" /SECDrawAs="Region" misc_signal    329..359 /gene="MCS'" /SECDrawAs="Label" CDS            complement (392..1321) /gene="araC" /SECDrawAs="Gene" CDS            1609..1914 /gene="ccdB" /SECDrawAs="Gene" misc_feature   1979..2405 /gene="oriVR6K" /SECDrawAs="Region" CDS            complement (2485..3279) /gene="aph" /product="KanR" /SECDrawAs="Gene"



>pSW8197

CAATTGATATCGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTAC CAGCGCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTT TTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGG TTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATA GGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTA TTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGG CGTCGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGAT CCACTAGTTCTAGAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAA CTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGAAACTCGC TCGGGCTGGCCCCGGTGCATTTTTTAAATACTCGCGAGAAATAGAGTTGA TCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTAGT GCTCAAAAGCAGCTTCGCCTGACTAATGCGTTGGTCCTCGCGCCAGCTTA AGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGC GACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGC CAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCG GTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGC TCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCC GGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTT CATCCGGGCGAAAGAAACCCGTATTGGCAAATATTGACGGCCAGTTAAGC CATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCA TTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGA ACAGCAAAATATCACCCGGTCGGCAGACAAATTCTCGTCCCTGATTTTTC ACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCC CAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCG TTAAACCCGCCACCAGATGGGCGTTAAACGAGTATCCCGGCAGCAGGGGA TCATTTTGCGCTTCAGCCATACTTTTCATACTCCCACCATTCAGAGAAGA AACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACT GGCTCTTCTCGCTAACCCAACCGGTAACCCCGCTTATTAAAAGCATTCTG TAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTA TAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTT GCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGC TTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTTGGATGGA GTGAAACGATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTAT CGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACG GATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCC GTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATG ACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGC TGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGT TCTGGGGAATATAAGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAAT TGCGCGCAATTCCCATGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAAT TGCTTTGAGAGGCTCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGT TGTCCACAACCGTTAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTT TTTTTCTTATAAAACTTAAAACCTTAGAGGCTATTTAAGTTGCTGATTTA TATTAATTTTATTGTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAG CTTAGTACGTTAGCCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGT TCGTTAAACATGAGAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGA AACATGAGAGCTTAGTACGTACTATCAACAGGTTGAACTGCTGATCTTCA GATCCTCTACGCCGGACGCATCGTGGCCGGATCAGATCTGATATCGTCGC AGACCAAAACGATCTCAAGAAGATCATCTTATTAATCAGATAAAATATTT CTAGGCACCAATAACTGCCTTAAAAAAACTGCAGTCAGAAGAACTCGTCA AGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTA AAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATAT CACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGG CCACAGTCGATGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTCGG CAAGCAGGCATCGCCATGGGTCACGACGAGATCCTCGCCGTCGGGCATCC GCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCT TCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTACGTGC TCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCAGGTAGCCGGAT CAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCG GCAGGAGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAA TAGCAGCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAGCACAGCTGCGC AAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGTCTTGG AGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAACCGGGCG CCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCT GTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAA CCTGCGTGCAATCCATCTTGTTCAATCATTTTAGCTTCCTTAGCTCCTGA AAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTAT GGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAA AAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACACCAGGATTTATTTA TTCTGCGAAGTGATCTTCCGTCACAGGTATTTATTCGGCG