IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/15

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Wednesday 15th September

 * The overnight digestions were checked using gel electrophoresis[]. Which was set up as shown bellow:

Lane 1) BBa_J04450 Lane 2) Ladder Lane 3) BBa_J04450 Lane 4) HapR-GFP Lane 5) CsrB-GFP Lane 6) pgaA-GFP Lane 7) CsrB-GFP Lane 8) pgaA-GFP
 * Starting from the top:




 * The small band from the lanes containing a cut promoter-GFP were extracted using the gel extraction kit.[]
 * The large Band from the lanes containing part BBa_J04450 were extracted using the gel extraction kit.[]


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