Maheshri:CommonArea

Sign-up for equipment usage

 * For heavily used equipment (Microscope, deep-well plate shakers), please sign-up in advance

Sink Usage

 * Do not leave items in the sink while bleaching.
 * Small items can be bleached on benchtop.
 * Large/many items can be bleached in fume hood and rinsed within 24 hours.
 * Clear all items from the sink area within 24 hours
 * Dry gel trays next to gel viewer away from ddH2O cord
 * Wipe down counter to keep area clean and dry
 * Soap and bleach refills are in cabinets under sink and water baths

Media Making Station

 * Put all reagents back after use
 * Clean up any spills
 * Make sure recipe book is neatly arranged
 * Throw away any trash

Balances

 * Clean balances after use
 * Don't leave spatulas lying around
 * Put reagents back after use
 * Throw away any weighing papers/trays
 * Throw waway any trash, even if it's someone else's mess

pH Meter

 * Turn on with probe still in buffer
 * Remove probe from buffer and rinse with ddH2O into rinse waste collection container
 * Measure pH
 * Rinse again after use
 * Replace probe in buffer before turning off
 * Empty rinse waste collection container
 * Clean up any mess you make

Gel Area
After running gel electrophoresis
 * 1) Turn off the UV lamp in the imager. Remove the pipette tip from the safety trigger.
 * 2) Remove the gel from the imager. To recycle the agarose gel, place it in one of the two designated 500mL containers (with purple caps) located in the gel area.
 * 3) To dispose the agarose gel, dump it into the five gallon black gel solid waste container located on the ground near the -80 freezer.
 * 4) Dispose the plastic wrap into regular biological waste and razor blade into the bench-top red sharps container.
 * 5) Used electrophoresis buffer can be directly dumped into the sink. To reuse the buffer, leave it in the gel box and cover the gel box to prevent evaporation.
 * 6) Rinse the plastic gel tray and dry it in the sink area.
 * 7) Measuring cylinders filled with buffer should be cover by Parafilm to prevent evaporation. Remove any empty measuring cylinders from the area.
 * 8) Clean up any spills immediately.

Plate Reader

 * Remove the microplate from the platereader after use. Don’t leave used microplates in the surrounding area.
 * Please DO NOT grow (with shaking) your cell cultures in the platereader. The platereader is not designed for cell culturing.