User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/01

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Check Transformation of BBa_I13504
Around 10 colonies where produced.

Preparation of M9 Media for YYC912
Yale Stock Centre Strain YYC912

1M MgSO4, 10ml
MgSO4x7H20 has a molecular weight of FW 246.48.


 * 1) To make 1M in one litre of water add 246.48g powder and increase volume by adding water to 1 litre.
 * 2) To make 1M in 10ml of water add (246.48g)/(1000ml/10ml) = 246.48g / 100 = 2.47g powder and increase volume by adding water to 10ml.

1M CaCl2, 10ml
CaClx6H2O has a molecular weight of FW 219.08.


 * 1) To make 1M in one litre of water add 219.08g powder and increase volume by adding water to 1litre.
 * 2) To make 1M in 10ml of water add (219.08g)/(1000ml/10ml) =219.08g / 100 = 2.19g powder and increase volume by adding water to 10ml

10% Casamino Acids
1g/l to make 10% Stock Casamino Acids 0.1g to make 100ml 10% Stock Casamino Acids

Setup overnight growth of BBa_I13504 colonies from plate
I prepared 3 bottles with ampicillin and inoculated with colonies from the transformation


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