IGEM:IMPERIAL/2009/Assays Protocols/Shopping List

= Shopping List=


 * Food grade sodium acetate : |SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Purchasing Page

Module 1
Sigma Products for Cellulase Assay:

No. S-8625 = Sodium Acetate, Trihydrate :|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Purchasing Page

No. S-3504 = Sigmacell Solution (Sigmacell) |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Purchasing Page

No. 16-10 = Glucose (HK) |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Purchasing Page

Sigma Products for PAH Assay: 

Trizma Base : Sigma Prod. No. T-1503

L-Phenylalanine : Sigma Prod. No. P-2126

Catalase : Sigma Stock No. C-100

DL-Dithiothreitol : Sigma Prod. No. D-0632

DL-6-Methyl-5,6,7,8-Tetrahydropterine Dihydrochloride : Sigma Prod. No. M-4758

Trichloroacetic Acid, 6.1 N Solution : Sigma Stock No.490-10

Nitric Acid : Aldrich Stock No. 25811-3

Sodium Nitrite : Sigma Prod. No. S-2252

1-Nitroso-2-Naphthol : Sigma Prod. No. N-3765

Sodium Hydroxide Solution : 1.0 N, Sigma Stock No. 930-65

L-Tyrosine Free Base : Sigma Prod. No. T-3754

Module 2
 Equipment required for Cell Colanic Acid Assay  PCV Centrifuge Tubes :Purchasing Page

 Trehalose 6-Phosphate Synthase Assay (OtsA)  Reagents: HEPES/ KOH (pH 6.8) MgCl2 ; Sigma prod no. m8266: |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Order Page Glucose-6-Phosphate; Sigma Prod no. G7772 : |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Order Page UDPG Bovine albumin; Sigma Prod. No. A8531 : |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Order Page Phosphoenolpyruvate ; Sigma Prod No. 860077 : |ALDRICH&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Order Page NADH Pyruvate kinase |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Order Page Lactate dehydrogenase; Prod. No. 59747 : |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Order Page

 Trehalose Assay  Standard Trehalose Test Kit : Megazyme Order Page Other required Reagents: Sodium Hydroxide Alkaline borohydride (10 mg/mL sodium borohydride in 50 mM sodium hydroxide); Sigma Prod. No. S9125 : |SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sodium Borohydride Sigma Order Page Acetic Acid

Module 3
 Cell Death Test  Cell Membrane Staining Kit Biotium Purchasing Page