User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/30

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Splitting cells, Immunoblot
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Summary

 * Split cells for RII overlay stimulation
 * Split cells for ELISA stimulation?
 * Dependent on how fast Alamar blue gets here
 * Antibody incubation for blot blocked ON
 * mouse anti-RIIα (1:5000) for 2 hours @ RT
 * Wash 3x 10 min. block buffer (1% BSA)
 * anti-mouse secondary antibody (1:10 000) 1 h @ RT
 * Rinse with block buffer
 * Wash 3x 15 min. w. block buffer


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