IGEM:Penn State University 2006/calendar/2006-6-27

=To Do: Tuesday=

Tony

 * restrict and run gel pSB4A3 (need more volume than yesterday)
 * gel extract, ligate, and transform IIR+motB constructs into pSB4A3
 * competency prep on RP437
 * transform RP437 and RP3087 with GFP
 * transform R0100+motB, C0051 (from registry plate) and pSB4A3 into DH5a

Jon

 * use Pat's program to create a map of the IIR+motB part in pSB1A2 and pSB4A3
 * restrict R0051 and motB, and run on gel
 * make LB AND SOB

Joel

 * Inoculate 6ml of inducible, 2ml constitutive, 2ml DH5-alpha at 11AM.
 * Plate reading on 9hr cultures at 8PM and 24 hr cultures at 10PM.
 * Read Drew Endy's plate reading assay protocals Endy lab page
 * inoculate 2ml of inducible (I14033 and I14032) and 2ml of constitutive.

Ralph

 * Plate read (Pat's Lab) see above
 * Read Drew Endy's plate reading assay protocals Endy lab page

Lane

 * Innoculate colonies from pLacIq+B0030/31/34+C0012 plates
 * ?competency prep on RP437?
 * Transform I14032+E0240 into RP437

Deepti

 * stuff