User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/24

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05/24/11

 * &#x2713; Cultures/minipreps/digests: 2 mL cultures for KAH183(2); KAH184(4)

Minipreps > Check with E/P digests

--> KAH184 not successful. Re-do with higher ratio of insert

Assemblies --> Re-build KAH184
 * 1) KAH184: HPK/(E/S)/516 + KAH182/(E/X)/4869

> Ligations

---> Add 15 μL to 40 μL DH5α Turbo


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