Lactobacillus miniprep

Overview
A miniprep for Lactobacillus delbruckii (development in progress)

Procedure

 * 1) Incubate cells for 30-45 minutes in TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose) with 10mg/ml lysozyme and 70U/ml mutanolysin.
 * 2) Perform normal QIAGEN miniprep
 * 3) verify extraction of plasmid by sequencing or digesting and running on gel

Contact

 * Derek Ju (derekju [at] mit.edu)

or instead, discuss this protocol.