User:Victor Tapia

Contact Information

Víctor  E. Tapia  Mancilla Molecular Recognition Laboratorium, Institut für medizinische Immunologie CHARITÉ - UNIVERSITÄTSMEDIZIN BERLIN

-  et   -

Equipe Cytosquelette d'actine et Trafic intracellulaire Département de Biologie moléculaire et celluilaire UMR7156 CNRS-Université de Strasbourg

Email me through OpenWetWare  last change on 01.04.2010

Research interests

 * Modularity of Protein Structure and Cellular Signal Transduction.
 * Protein Folding and Neurodegenerative Deseases.
 * Natural Autoimmunology and Diagnostic Assays

Publications

 * 7 - Volkmer R, Tapia V. Exploring Protein-Protein Interactions with Synthetic Peptide Arrays. In: SPOT Light on Peptide Arrays on Solid Supports – Applications and Perspectives. Bentham Science Publishers; 2011. p. 164-170.
 * 6 - Tapia VE, Nicolaescu E, McDonald CB, Musi V, Oka T, Inayoshi Y, Satteson AC, Mazack V, Humbert J, Gaffney CJ, Beullens M, Schwartz CE, Landgraf C, Volkmer R, Pastore A, Farooq A, Bollen M, Sudol M. (2010) "Y65C MISSENSE MUTATION IN THE WW DOMAIN OF THE GOLABI-ITO-HALL SYNDROME PROTEIN PQBP1 AFFECTS ITS BINDING ACTIVITY AND DEREGULATES PRE-mRNA SPLICING." J Biol Chem.;285(25):19391-401. Epub 2010 Apr 21.
 * This work explores the effect of point mutation leading to X-LMR at the reductionist level of domain-peptide interactions, inferes potential affected functions, and tests inferences on pre-mRNA splicing
 * 5 - Mahrenholz CC, Tapia V, Stigler RD, Volkmer R. (2010) "A STUDY TO ASSESS THE CROSS-REACTIVITY OF CELLULOSE MEMBRANE-BOUND PEPTIDES WITH DETECTION SYSTEMS: AN ANALYSIS AT THE AMINO ACID LEVEL." J Pept Sci.;16(6):297-302.
 * ever wanted to see if your favorite detection system reacts with cellulose-bound peptides?
 * 4 - Tapia VE & Volkmer R (2009) "EXPLORING AND PROFILING PROTEIN FUNCTION WITH PEPTIDE ARRAYS" in: Methods in Molecular Biology, Vol 570 (Eds. M Cretich & M Chiari). Humana Press. - ISBN: 978-1-60327-393-0.
 * This work reviews the peptide array technology focusing on technological advancements, oriented immobilization of peptide probes, and some interesting applications. Going along the philosophy of the monography series, we give some general recomendations on methods and instruments. See the following newsletter comments by Tony Fong: Q&A: Raising the Profile of Peptide Arrays for Studying Protein Function, ProteoMonitor, August 13, 2009
 * 3 - Tapia VE, Ay B, Triebus J, Wolter E, Boisguerin P, Volkmer R. EVALUATING THE COUPLING EFFICIENCY OF PHOSPHORYLATED AMINO ACIDS FOR SPOT SYNTHESIS. J Pept Sci. 2008 Dez ;14(12):1309-1314. - PMID: 18816512.
 * This work was carried out to adapt "building block" approaches for the generation of phosphopeptides through resine-supported solid-phase peptide synthesis to the spot-technology, which relies on cellulose as support. It compares different coupling strategies for the incorporation of protected phoshoaminoacids into determined positions of synthetic peptides. We find that the use of EEDQ (caution, neurotoxic!) as coupling activator is the most efficient strategy in our comparison.
 * 2 - Tapia VE, Bongartz J, Schutkowski M, Bruni N, Weiser A, Ay B, Volkmer R, Or-Guil M. AFFINITY PROFILING USING THE PEPTIDE MICROARRAY TECHNOLOGY: A CASE STUDY. Anal Biochem. 2007 Apr 1;363(1):108-118. - PMID: 17288979.
 * This work analyses the predictive potential of microarray-based binding assays. Fluorescent SI-measurements were used to fit a mass-action derived model estimating the observed affinity (equil. diss. const.).
 * 1 - Weiser AA, Or-Guil M, Tapia VE, Leichsenring A, Schuchhardt J, Frommel C, Volkmer-Engert R. SPOT SYNTHESIS: RELIABILITY OF ARRAY-BASED MEASUREMENT OF PEPTIDE BINDING AFFINITY. Anal Biochem. 2005 Juli 15;342(2):300-311. - PMID: 15950918.
 * This work analyses the quantitative potential of measurements derived from peptide macroarray-based immunobinding assays. Chemoluminiscent signal intensity data was confronted with independent "golden stardard" measurements (surface plasmon resonance-based binding assays on a BIAcoreX maschine).

Presentations

 * 6 - V.E. Tapia & R. Volkmer; Steady State Analysis of Peptide Array-based Binding Assays: A Mathematical Model for Disease Diagnostic and Affinity Profiling of Bimolecular Interactions. 9th German Peptide Symposium, 2009, Göttingen.
 * 5 - Mahrenholz CC, Tapia V, Stigler R, Volkmer R; Peptides crossreacting with detection systems – Analysis at the amino acid level. 9th Peptide Symposium (2009).
 * 4 - V.E. Tapia, C. Landgraf, A. Pastore, M. Sudol, R. Volkmer; Analysis of a missense mutation in the WW domain of PQBP1. FEBS Workshop 2007, Protein Modules and Networks in Health and Disease, Seefeld, Austria.
 * 3 - V.E. Tapia et al; titel; 2008 Brownschweig, Germany
 * 2 - V.E. Tapia et al; titel; 2007 Frankfurt, Germany
 * 1 - V.E. Tapia & M. Or-Guil; Reading Natural Autoimmune Responses with Random Peptide Arrays; Workshop "Strukturbildung in Chemie und Biophysik", Salzwedel, Germany, October 2004.

Useful links

 * Introductory tutorial
 * OpenWetWare help pages
 * Molecular Recognition Lab
 * AG-Volkmer official site
 * wiki editing

Who's visiting
start on 18 Mar 2010

 

Science in the city
This is a short series of high content essays about the every day life ''of scientists in berlin. If you really have the time, or your boss is'' not around at the moment, then read ahead and enjoy. ATTENTION: technical problems (work) are keeping me away from my mission but i will return tu fun as soon as possible. DAY 1 - ANALYTE CAPTURING DAY 2 - INTO THE SKY OF BERLIN DAY 3 - MATTERS OF AFFINITY