Koeris/Notebook/2007-1-16

As one finds often, good planning is half the work. Now I need to re-transform both A and B constructs into K12 EMG2 cells in order to make more plasmid, since I can't find the stocks. On the other hand I did find the 06 stock but no plasmid prep... So I got to grow that one up again, too. Bottom line: wasted a day.

=Sequencing of [His]6 constructs= All three constructs need to be sequenced. New sequencing service in use at Boston University: GeneWiz.

Samples need to be dropped off BEFORE 4pm at Larry Valles office LSEB601.

Set up of sequencing
IMPORTANT: DILUTE STOCK PRIMERS to 2pmol/ul

Measure concentration of all 3 constructs
 * 06
 * 07
 * 08

Sample set up guidelines

 * Submit your samples in 0.2 ml, 8-strip PCR tubes, one sample per tube.
 * For the amount of template needed, please refer to the following table.
 * Provide 8 microliters (without primer) or 12 microliters (premixed with primer) of DNA per tube.

Label Sample Tubes
Please label your tube on the SIDE with the corresponding sample ID. Cap your 8-strip tubes tightly with matching 8-strip caps. Try to provide some cushion around the tubes to avoid any potential damage during shipping.

Send Samples
Send your samples along with a printed copy of the order summary to GENEWIZ via the preferred overnight courier service at FedEx.