Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/13

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13 October 2009 Lab Meeting
‡ Announcements ‡ Flu R01:Integration † Sample Concentration (Lead: Jane, Team: Jaephil)
 * Attending:
 * Missing:
 * Presentation: Dr. Qian Mei, Post Doc Applicant, 2pm, please BE ON TIME.
 * Invited speaker at group meeting on 10/20 - PLEASE Do NOT MISS - Dr. Tushar Bansal, post doc applicant.
 * PAPERS Drafted before MicroTAS
 * Posters for MicroTAS Due to CMK by 10/15
 * Posters for MicroTAS Due to CMK by 10/15
 * New design - address evaporation loss, Reagent delivery and storage solution. Consider marginating flows?
 * Up to high 10^7 for positive control. Silver substrate no signal.
 * Jane working on the cell lysate control: plan to have Cassidy's help on plaque assay while I'm away.
 * New cell passage from frozen - the old one is beyond passage 30.
 * Main loss is at the outlet/sample collection: tangential filtration design in drawing stage.
 * Contacted EC Shaw about rubber stamp mold for new design for evaporation.
 * Set up COMSOL and StarCD, look for governing equations for simulation of evaporation.
 * Committee meeting setup

† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) † PCR - CMI (Lead: Qingqing) † HDA (Lead: Jaephil, Team:Sonali) '''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc ) ‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing) ‡ Agilent Automated Sample Preparation (Lead: Alex) ‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)  ‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks) ‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA) ‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)
 * Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. Deliver CAD drawings for these for photomasks by Friday 10/16, functional chips to be delivered by 10/26 [Hussam/Jessie]
 * Silica free SPE, and SPE free channel experiment to be repeated. Control was old didn't take pressure, report back on 10/15.
 * Load DNA in a straw of 50ul SPE, quick test of hypothesis more SPE = more DNA, report back 10/16.
 * QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR.
 * PCR2 Paper formatted for LOAC this week.
 * CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations.
 * Start planning R01 for Submission on 2/5/10. MM, JD, CMK.
 * JD will train QQ how to make HDA chips. This may entail moving from cutter plotter to embossing steps. JD and QQ to meet ASAP to plan the tech transfer.
 * Paper submitted 10/6.
 * Sonali to train Lisa on SPE.
 * QQ to run test PCR on chip with genomic DNA and Toxin B primers.
 * Meeting 9am Monday (every other week)
 * With Straws in Parallel at this point. Speeding up the assay.
 * Alex will have drafts this week.
 * Metal piece modification for the integration - Parts distored and is begin fixed.
 * paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli.
 * New experiments happening now.
 * Cathie will submit paper inquiry.
 * IRB approved.
 * Cathie submitted the companion BU IRB form for exemption. Revised, still still waiting.
 * Phone meeting with PATH this week. 10/13.
 * Training on straw making concluded.
 * UGs purchasing parts for straw machine for 709.


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