WangLab:Transformation Procedure

Transformation Procedure

 * 1) Take ice, put DH5a, wait to thaw
 * 2) Aliquote 50 ul to prechilled 600 ul tubes
 * 3) Add 1 ul DNA to tubes and wait for 30 min on ice.
 * 4) Heat shock 42oC for 45 sec.
 * 5) Incubate on ice for 2 min
 * 6) Add in LB medium 500 ul to each tube
 * 7) Shaker in 37oC for 20 min
 * 8) 50 ul plate on agar plates