User:Guido/Notes/July 07

July 3, 2007

 * Do a culture pcr from last recombination of specR to replace MutS


 * 3 &mu;l culture
 * 1 &mu;l f-primer
 * 1 &mu;l r-primer
 * 45 &mu;l supermix
 * 50 &mu;l reaction

* 95 10 min * 95 30 sec * 51 30 sec * 72 2 min * X30 * 72  7 min * 4 forever