Mesoplasma florum:Primer extension

Goal: identify transcription start sites of the entire genome

 * Isolate total RNA
 * Reverse transcribe the RNA using a 5' fluorescently labeled primer
 * Isolate ssDNA
 * Mix ssDNA into a standard sequencing reaction
 * Sequence the mixed product

Fluorescent labels

 * LIZ standard
 * VIC or HEX
 * NED or TAM
 * FAM
 * PET
 * ROX standard
 * VIC or HEX
 * NED or TAM
 * FAM
 * FAM is cheap and suitable

Custom Genescan markers

 * Bioventures, ROX, TAM labeled