User:Tara K. Luckau/Notebook/Team ConGen/2010/11/05

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 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
 * style="background-color: #9DB68C" align="center"|  |Main project page
 * style="background-color: #9DB68C" align="center"|  |Main project page


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Cast Gel

 * 270µL 1x TAE + 5.4g agarose
 * hot plate + stir bar
 * 27µL Gel Red (added when agarose turned clear but still hot, used stir bar)

Load Gel

 * 5µL loading dye + 5µL sample
 * [[Image:20101105 GelSchematic.jpg|800 px]]

Run Gel

 * 130V, 2hrs

Image Gel

 * UGH! Why ain't my shit workin'?
 * Top:
 * [[Image:20101105 Top.TIF|300 px]]  [[Image:20101105 Top2.TIF|300 px]]
 * [[Image:20101105 TopZoom.TIF|300 px]]
 * Bottom:
 * [[Image:20101105 Bottom.TIF|300 px]]
 * [[Image:20101105 BottomZoom1.TIF|300 px]]  [[Image:20101105 BottomZoom2.TIF|300 px]]

Results

 * Very faint bands at E2, E4, E6, corresponding to DNA concentration of 1x, Buffer G, Temperatures 55, 57, 59
 * around 150 bp band


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