IGEM:Yale/2010/Notebook/2010/07/05

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Colony PCR
This day's activities are also recorded on page 47 and 48 of the hard copy lab notebook.
 * Ran colony PCR of colonies 1B-9B from ligation attempt #2 according to the same protocol as used on  7/1.
 * Then ran a 1.0% agarose gel of the nine PCR reaction solutions against a 1 kb ladder, but the resulting gel failed to show any evidence of either insert or vector, so will have to rely on sequencing and digests of miniprepped plasmids. [[Image:050710_ligationproducts.jpg|thumb| Gel of colony PCR from ligation attempt #2 Lane 1: 1 kb ladder, Lanes 3-11:PCR products from colonies 1B-9B, in order]]
 * Miniprepped liquid cultures grown from colonies of first ligation attempt (colonies 1A-4A) and second ligation attempt (colonies 1B-9B) according to standard miniprep protocol.


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