IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/12

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Team Allergy
ihpRNA


 * Minipreps of more V9/V10 colonies
 * Diagnostic Digest of minipreps w/ Eco and Pst (Should see ~840 and 7000 bp)

ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b

Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2)


 * Digested V9/V10 backbone for ligations w/ Eco & Pst (redoing ihpRNA ligations into V9/V10)

amiRNA


 * Ligated into V9/V10 and transformed (mutagenesis sites confirmed through pcr using 20 bp sepecific primers)
 * still need to be sequenced

[[Image:

Team Fence


This picture is not the best picture but we did find colonies on the plate with the arp2/b21 transformation.



transformation from karmella's stock

Transformations from Yesterday's Ligations


Colonies beyond control on RXRLc VP16, Barstar V26, and EcR-Gal-PhyB.


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