Template:SBB10Project-11097

Joanna Chen

Overview
Your two parts are very similar and they require gene synthesis. In designing your oligos, you should codon optimize the gene sequence for E. coli and try to maximize re-use of your oligos in making this. You might need to write a small program to help you generate this, and JCA can provide you some starter files to help you out.

sbb17: Zinc Finger Domain (zf+)
Source: Synthetic Target Sequence:   MAPKKKRKVGIHGVPAAMAERPFQCRICMRNFSRSDTLSEHIRTHTGEKPFACDICGRKFAARSTRTTHTKIHTGSQKPFQCRICMRNFSRSDSLSKHIRTHTGEKPFACDICGRKFAQRSNLKVHTKIHLR Vector: pBjk2741 Short desciprion: {} Flavor: { } Family: Zinc Finger

sbb18: Zinc Finger Domain (zf-)
Source: Synthetic Target Sequence:   MAPKKKRKVGIHGVPAAMAERPFQCRICMRNFSRSDNLSVHIRTHTGEKPFACDICGRKFARNAHRINHTKIHTGSQKPFQCRICMRNFSRSDTLSEHIRTHTGEKPFACDICGRKFAARSTRTNHTKIHLR Vector: pBjk2741 Short desciprion: {} Flavor: { } Family: Zinc Finger