IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/08/Min Lin

The Primer(Made of Standard Prefix and Suffix arrived). Add ddH2O to each tube.

PCR tetR plasmid (MasterMix) to see whether the primers work. PCR protocol. ( the Band is too narrow which means that the PCR is not done very well)

Try Colony PCR No Signal

Go to Lingli’s Lab, do gradient PCR. 52, 53, 54, 55, 56, 57, 62, 64

All of the temps works well.

It turns out that the thermocycler in our lab has no hot cap. So the liquid is on the tube cap when exposed to 94 centigrade.