IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/16

{| width="800"
 * style="background-color: #800000"|[[Image:UIUC_ArsR_System.jpg|150px]] UIUC - Arsenic Collection System!
 * style="background-color: #AFEEEE" align="center"|  |Main project page
 * style="background-color: #AFEEEE" align="center"|  |Main project page


 * colspan="2"|
 * colspan="2"|

9/16/10
All overnights were white!!!

12:30 PM

Miniprep Promega kit

J23100B0034ArsRSDM2B0015pSB1T3

colony 1: 76.4 ng/μL

colony 2: 75.9 ng/μL

colony 3: 6.5 ng/μL

colony 4: 50.2 ng/μL

diluted colonies 1,2, and 4 to 20 ng/μL

1:30 PM

Verification PCR

Reaction for J23100B0034ArsRSDM2B0015pSB1T3 3

Samples:  J23100B0034ArsRSDM2B0015pSB1T3 1 J23100B0034ArsRSDM2B0015pSB1T3 2 J23100B0034ArsRSDM2B0015pSB1T3 3   J23100B0034ArsRSDM2B0015pSB1T3 4  negative control (H2O) 

Primers (10 micromolar):   VF2</li>  VR</li> </ul>

Program:

5:20 PM

Gel

TAE, 1%, 175V



Made glycerol stock of J23100B0034ArsRSDM2B0015pSB13 colony 1, ISB237


 * }