User:Fermenter User/Notebook/BROM G2

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Background color codes:  Pastel Green: Major change of setup this time  yellow: Sung-Hye's correction or question to user

Letter color codes: Black: Basic comment Red: Emergency Information (Power shutdown, building access, Manager's absence etc) Blue: Fermentation Setup information Green: Discussion Orange: Fermentation hrs

BROM G2 (F4)

Day 0 (Feb 2 Mon):
           2/2/2009 (10:45) Bring media to fermenter room           2/2/2009 (11:00) Mix powder with 30-L water 2/2/2009 (11:00) Add 30-ml of Antifoam 2/2/2009 (11:15) Acid (500 ml) and Base (1000 ml) 2/2/2009 (11:25) Start Sterilization recording 2/2/2009 (11:30) Start Sterilization Cycle 2/2/2009 (12:25) Cooling of bioreactor (Temp setup) 50 L/min air, 250 rpm 2/2/2009 (12:27) Start Fermentation Software for Fermenter #4 2/2/2009 (12:53) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min 2/2/2009 (12:53) Set dO2 2/2/2009 (12:54) Start pH control 2/2/2009 (12:55) Completion of bioreactor set up: Ready for inoculation Natasha, no need to bring EtOH!! -               2/2/2009 (13:00) Inoculation: inoculation volume (900 ml) Fermentation 0:00            2/2/2009 (13:00) Sampling  Fermentation 0:00 Cell weight (2 mg), Activity ( 16%, x100), Activity ( 3%, x1000)

Day 1 (Feb 3 Tue):
Sung-Hye: CHBE Class Auditing 9:30 - 11:00

           2/3/2009 (8:15) Fermentation 19:15 Acid (420 ml) Base (590 ml) 2/3/2009 (8:15) Change configuration (stirrer external)             2/3/2009 (9:00) Sampling  Fermentation 20:00 Cell weight (155 mg), Activity ( 61%, x100), Activity ( 23%, x1000)            2/3/2009 (10:00) Fermentation 21:00 Refill Base (500 ml: total 1100 ml)             2/3/2009 (14:00) Sampling  Fermentation 25:00 Cell weight ( 231 mg), Activity ( 78%, x100), Activity (28 %, x1000)            2/3/2009 (14:20) Fermentation 25:20 Refill Acid (500 ml: total 620 ml) Base (1000 ml)             2/3/2009 (18:00) Sampling  Fermentation 29:00 Cell weight ( 202 mg), Activity ( 82%, x100), Activity (40 %, x1000)

Day 2 (Feb 4 Wed):
<Sung-Hye>           2/4/2009 (9:15) Fermentation 45:15 5 am - 9:15 am : O2 tanks ran out Open O2 tank Acid (650 ml) Base (100 ml) <Natasha>            2/4/2009 (10:30) Sampling  Fermentation 46:30 Cell weight ( 369 mg), Activity ( 79%, x100), Activity (41 %, x1000) <Sung-Hye>           2/4/2009 (10:30) Fermentation 46:30 Refill Base (500 ml: total 550 ml) <Sung-Hye>           2/4/2009 (11:30) Fermentation 47:30 Finish Fermentation and Ready for Harvest with Continuous Centrifuge Acid (650 ml) Base (550 ml) <Sung-Hye>           2/4/2009 (11:45) Start Centrifuge 2/4/2009 (11:50)                         First 4-L of media was poured back to fermenter 2/4/2009 (12:30)                         Centrifuge completed


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