Griffitts:Plant DNA preparation

Materials

 * Plant material
 * Liquid nitrogen
 * Pestles
 * Ice
 * 70% ethanol
 * Qiagen Cell Lysis Solution (on shelf with Qiagen kits)
 * Qiagen Protein Precipitation Solution (on shelf with Qiagen kits)
 * T3E0.3
 * 1.7-mL microcentrifuge tubes

Procedure
Optional:
 * Cut 1 leaf from plant and place in a microcentrifuge tube
 * Dip in liquid nitrogen for 1 second, pour off excess
 * Right as the liquid nitrogen boils off, add 500 μL Qiagen Cell Lysis Solution
 * Grind with pestle
 * Vortex 5 seconds
 * Heat at 65°C for 1 hour (vortex occasionally)
 * Chill to room temperature on ice
 * This takes about 4 minutes
 * Add 180 μL of Qiagen Protein Precipitation Solution
 * Vortex for 20 seconds
 * Incubate on ice for 15 minutes
 * Centrifuge at maximum speed for 6 minutes
 * Move 500 μL of the supernatant to a new tube
 * Add 500 μL isopropanol
 * Invert 3 times
 * Wait 10 minutes
 * Centrifuge at maximum speed for 6 minutes
 * Pour off supernatant
 * Resuspend in 70% ethanol
 * Vortex for 3 seconds
 * Centrifuge at maximum speed for 6 minutes
 * Pour off supernatant
 * Centrifuge at maximum speed for 30 seconds
 * Remove remaining supernatant using a P-200
 * Add 80 μL T3E0.3
 * DON'T pipette up and down!
 * Heat at 65°C for 5 minutes with flicking
 * Run 5 μL of each sample on an electrophoresis gel