User:Karmella Haynes/Notebook/Polycomb project/2010/03/09

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03/09/10

 * &#x2713; Chromatin IP: cross-link KAH127-4, 128-8, 130-2, 132-8
 * &#x2713; Dilution plates for selecting RFP+ colonies w/ improved expression: KAH131-9, KAH128-8

Chromatin IP: Cross-linking > Check for RFP expression > Qingqing's dual cross-linking protocol (DMA and formaldehyde cross-linking) > Use 5x 15 cm plates (~2x107 cells/ plate) > Freeze cells in liq. nitorgen and store at -80°C


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