User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/09

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] hTERT stimulation @ splitting, Western blot contn'd
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Summary
Split D12 (p25) and D9 (p21) to 4 new petridishes (p+1) 24-wells D9 was put to S0 2x 6 wells (d12, d9) were refreshed with S+

Western blot Primary antibody (1:1000 in 5% milk) (rabbit anti AKAP-79), 2 hours RT wash 3x secundary antibody (1:1000, 5% milk) (goat? anti-rabbit),1 hour RT wash 3x ECL, inc. 5 min. take pictures every 5 min, 6x GAPDH 1:2000 ab for control protein amount

Stimulation D12 24 wells, S0/Ht31

Results
PICTURE --> ANOUK

Conclusion

 * ✅ Ow..

Same topics

 * 10Feb2010 Splitting cells


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