IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/11/Zhangs

Store the strain of 1J①、11N②、2I①、1H④、2M①、5N①、2G① and send to sequence 7 samples in total to be sequenced: T7P+1J+CI+T①; T7P+5N+CI+T①; T7P+2G+CI+T①; T7P+2I+CI+T①; T7P+2M+CI+T①; T7P+11N+CI+T②; T7P+1H+CI+T④. The results demonstrates that T7P+1J+CI+T①; T7P+2I+CI+T①; T7P+1H+CI+T④; T7P+2M+CI+T①; T7P+2G+CI+T①; ARE THE TARGET PRODUCTS. While T7P+5N+CI+T①; T7P+11N+CI+T② failed.

Miniprep for 5J+CI①~⑤ and T7p+2K①~⑤ Electrophoresis to identify Result 1 (from left to right) T7p+1-2K+CI+T①-⑤(plasmid), T7p Plasmid Control, Marker The T7p+1-2K+CI+T①-⑤ are the target plasmids. Result 2 (from left to right) 1-5J+CI+T①-⑤(plasmids), RBS Plasmid Control, Marker Double digestion for 1-5J② insert: