User:Fermenter User/Notebook/MOLD A10

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Mold A10 (F3)
Day 0 (Oct 17th Fri):           10/17/2008 (11:08)      Start Autoclave Cycle Started  10/17/2008 (13:00)     Drain PBS, transfer media           10/17/2008 (13:18)      Start BioXpert software  Recording Time 0:00           10/17/2008 (13:45)      Completion of Bioreactor setup for inoculation              10/17/2008 (16:15)      Inoculation: ( 2,3*10^6/ ml,   80    ml) Fermentation Time 0:00              10/17/2008 (22:06)      Cell counts: ( 0,23*10^6/ ml) Viability (  95 %)  Fermentation Time 5:51

Day 1 (Oct 18th Sat):              10/18/2008 (16:44)      Cell counts: ( 0,20*10^6/ ml) Viability (  95 %)  Fermentation Time 24:29

Day 2 (Oct 19th Sun):              10/19/2008 (14:30)      Cell counts: ( 0,21*10^6/ ml) Viability (  95 %)  Fermentation Time 46:15

Day 3 (Oct 20th Mon):              10/20/2008 (13:30)      Cell counts: ( 0,28*10^6/ ml) Viability (  95 %)  Fermentation Time 69:15

Day 4 (Oct 21st Tue):  10/21/2008 (13:18)     Finish BioXpert 10/21/2008 (13:30)     2 x UPS are installed 10/21/2008 (13:22)     Start BioXpert              10/21/2008 (13:30)      Cell counts: ( 0,50*10^6/ ml) Viability (  95 %)  Fermentation Time 93:15

Day 5 (Oct 22nd Wed):              10/22/2008 (13:45)      Cell counts: ( 0,57*10^6/ ml) Viability (  95 %)  Fermentation Time 117:30

Day 6 (Oct 23rd Thu):              10/23/2008 (13:00)      Cell counts: ( 0,99*10^6/ ml) Viability ( 95  %)  Fermentation Time 140:15

Day 7 (Oct 24th Fri): <Frank>             10/24/2008 (13:50)      Cell counts: (1,60*10^6 / ml) Viability ( 95  %)  Fermentation Time 165:35

Day 8 (Oct 25th Sat): 10/25/2008 (16:47)     O2 valve opened 100% <Frank>             10/25/2008 (17:00)      Cell counts: ( 2,33*10^6/ ml) Viability (  95 %)  Fermentation Time 192:45 impeller speed increased to 200 rpm

Day 9 (Oct 26th Sun): <Frank>             10/26/2008 (16:00)      Cell counts: ( 1,93*10^6 / ml) Viability (  90 %)  Fermentation Time 215:45 increased O2 flow to about 200ml/min

Day 10 (Oct 27th Mon): 10/27/2008 (5:00 - 8:00) HVAC system test (No access to building, No expected power shut-down) <Frank>             10/27/2008 (12:00)        Cell counts: (1,73*10^6 / ml) Viability ( 85  %)  Fermentation Time 235:45 <Sung-Hye>          10/27/2008 (13:45)        Terminate Fermentation   Fermentation Time 237:30


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