Acinetobacter ADP1

Cultures

 * ADP1 can be grown anywhere between room temperature and 37°C
 * Will not survive at 4°C
 * For long term storage, add 10% DMSO and store at -80°C
 * Naturally resistant to ampicillin and chloramphenicol. Use spectinomycin, kanamycin, or tetracycline for selections.

Transformations
(derived from Metzgar et al. paper)
 * Streak out cells on LB plate, grow overnight at 37°C
 * Innoculate colonies into 2 mL LB and grow for 12-16 hours at 30°C with shaking
 * Take a 20 μL aliquot and add to 280 μL fresh LB
 * Add DNA
 * Shake at 30°C for 2 hours
 * Plate on appropriate media with antibiotic and let grow at 30°C

Constructs

 * 2.1 in plasmid IMBB
 * IMBB expresses TEM-1 β-lactamase and has a ColE1 origin
 * 2.2 in plasmid IMBB
 * 2.1-P1010-2.2 in plasmid pSB1AC3
 * 2.1-J04450-P1010-2.2 in plasmid pSB1AC3
 * gusA-specR reporter system