Calendar:ImperialWetLabNotebook2009iGEM/2009-9-2

--Dineka Khurmi 08:57, 2 September 2009 (EDT)

 WEDSDAY JOB LIST 


 * WaaL - Gel extraction - Run on gel with vector AK3 - Ligation


 * Harvard BB - Gel extraction - Run on gel with GFP - Ligation


 * CRP1 (vector) - Gel extraction - Run on gel with GFP - Ligation


 * Miniprep cellulase - Digestion - Run on a gel


 * Midiprep RFP I13507 (small culture)


 * Dirty ligation RBS + AK3 - Transformation


 * Pick culture colonies - grow 5ml


 * Make agar for Amp/Kan plates and LB media (send to autoclave)

David Roche 05:55, 2 September 2009 (EDT) AM : Ran a gel with the WaaL pcr products that were digested overnight. 20ul against the ladder Updated wiki and BioBrick registry


 * James Field 16:17, 2 September 2009 (EDT):

AM:

responsible for poor results.
 * Checked transformed plates from yesterday - only OtsA and Ots B had colonies (1 or 2 each). Positive control worked indicating that the ligation was


 * Did a mini Prep on the Edinburgh cellulases:

K118022

K118023

K118028


 * Went through and made corrections to SLIC primers for Construct 9.

PM:


 * Did Restriction digest on the Edinburgh cellulases and ran on gel. This indictated that the miniprep had been successful.


 * Plated OtsA and OtsB colonies from the Ak3+insert ligation plates (see AM for more details). Also kicked off a starter culture for a mini prep for these colonies which will be conducted tomorrow.


 * I transformed cells with the RBS + AK3 construct and plated out - will see results tomorrow following overnight incubation.


 * Innoculated media (200ml LB) for overnight incubation for tomorrow's midi on:

RBS-RFP-TT (BBa_I13507)

K118022

K118023

K118028