User:Meng Xiao He/Notebook/fall08/2008/10/31

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Update

 * BsmBI digest did not over digest (band sizes still correct)
 * T4 ligation for 15 minutes made DNA smear into large (8-10Kb) region, so over ligated

Next

 * repeat
 * PCR fragments over using existing fragments
 * purify>dephosphorylate fragments>purify>digest with BsmBI>ligate for 1-5 minutes>purify>XmaI digest
 * try crossover PCR

PCRs:

1-6: Xover:
 * cbb, cco, Duo; 3 then 5
 * cbb, cco, Duo


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