User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/25

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
 * style="background-color: #F2F2F2" align="center"|  |Main project page
 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * Dissected Experimental and control flies that were heat shocked on Thursday.
 * Fix with 4% Formaldehyde PBS Solution
 * 30 minutes
 * Wash 3 x 5 minutes in 1x PBS-T
 * Incubate 30 minutes with PBS 0.6% Triton.
 * Wash once with 1x PBS-T
 * Block in 2% NGS
 * O/N at 4C


 * }