IGEM:Harvard/2007/Protocols/Fluorescent Labeling Protocol


 * 1) Resuspend up to 10^7 nucleated cells in 100 ul of buffer.
 * 2) Add 10 ul of MACS Fluorochrome-conjugated Antibodies.
 * 3) Mix well and refrigerate for 10 minutes in the dark.
 * 4) Wash cells by adding 1-2 ml of buffer per 10^7 cells and centrifuge at 300 x g for 10 minutes. Aspirate supernatant completely.
 * 5) Resuspend cell pellet in a suitable amount of buffer for analysis by flow cytometry or fluorescence microscopy.