Template:SBB10Project-15924

Wei-Chun Hsu

sbb11: sleeping beauty 5'TR
Source: synthetic Target Sequence: agttgaagtcggaagtttacatacacttaagttggagtcattaaaactcgtttttcaactacaccacaaatttcttgttaacaaacaatagttttggcaagtcagttaggacatctactttgtgcatgacacaagtcatttttccaacaattgtttacagacagattatttcacttataattcactgtatcacaattccagtgggtcagaagtttacatacactaa Vector: pBjk2741 Short description: sleeping beauty 5'TR   Family:  Terminal Repeat

Proposed synthesis route: Fully Synthetic (GeneDesign) Proposed construction file (draft): Template:SBB10 ConstructionFiles JimH sbb11

sbb13: PhiC31 Integrase
Source: pBca9523-Bca1623, pBca9523-Bca1659 Target Sequence: DTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDGGRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIPIVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQRELGGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWREIKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRILRDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQAWLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPGQHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERANLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAEAPKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPIEKRASITWAKPPTDDDEDDAQDGTEDVAA Vector: pBjk2741 Short description:  Genbank reference: X59938.1 Flavor: { } Family: Phage Integrase A template for making this part has been constructed as two synthons in plasmids pBca9523-Bca1623 and pBca9523-Bca1659. You will need to use SOEing to splice these two synthons together. Take special note that you are creating the correct flavor for this part.

Proposed synthesis route: Modified SOE procedure Proposed construction file (draft): Template:SBB10 ConstructionFiles JimH sbb13