IGEM:PennState/2007/Labbook/GarrettTobin/2007-7-26

July 26th Plan for xylR
 * Make 6 different promoter+RBS+xylR constructs with different levels of expression
 * 2 different RBS - B0033 (stregnth .01) - B0034 (streght 10
 * 3 different Promoters - 1, 200, 2000

Today:
 * Transform xylR orf from miniprep
 * Transfrom B0033
 * Both into dh5a
 * B0033 dna from regrestry plates is in my box- labled as exactly that
 * Made sure there was a miniprep for other RBS-B0034\
 * 12:30-5:30

Tomorrow
 * Innoculate
 * Prepare Transformation/Find Minipreps of 3 promoters

Saturday
 * Miniprep, Restrict, Purify, Ligate, Transform
 * Innoculate promoters if needed

Sunday
 * Innoculate