User:Alexander S Mikheyev/Notebook/Midge genome/2010/04/16

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Tagmentation

 * mix on ice
 * incubate 5 min @55C
 * purify with minelute kit from quiagen and elute into 10 ul buffer

PCR

 * mix on ice
 * 72C 3 min; 95C 3min (95C 5s, 62C 30s, 72C 1min)x12, 72C 5 min


 * purified using quiagen pcr kit, using 2 washes
 * eluted in 50 ul, 6.5ng/ul

Gel

 * 3ul of non-purified PCR