User:Karmella Haynes/Notebook/Polycomb project/2010/09/12

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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09/12/10

 * &#x2713; p16 induction time course: add dox to 1-day plates (9 pm)
 * &#x2713; Plasmid cultures: grow 100 mL cultures for KAH160/015 and 170/015 midipreps
 * &#x2713; Cell culture: split confluent cultures
 * &#x2713; Cell culture: expand 158-3 (158-2 failed)
 * &#x2713; Thaw: HEK Gal4-EED again; pellet in 9 mL FBS, grow in 2x FBS DMEM (last two vials)
 * &#x2713; Nanostring assay: start new cultures in 10 mL plates


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