IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/09/07

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dspB Track

 * cPCR on plates (Streaked)

Colony PCR
PCR Tubes: His, no his, no his A, His A, W
 * Pick colony from respective plate and place in respective tubes

PCR Cycles:
 * 98C @ 3min
 * Cycle 27x:
 * 98C @ 10 sec
 * 72C @ 30 sec
 * 72C @ 40 sec
 * 72C @ 10 min
 * 10C @ hold

Start: 6:46pm End: 7:49pm

Gel verification colony PCR products
Gel orientation: Results:
 * Protocol: gel verification protocol in Protocol (SOP)
 * Changes: 1% agarose gel
 * Machine conditions: 0.5x TBE buffer, 100V, 60min


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