Knight:Reconstituting primers

Procedure
Invitrogen recommends the following reconstitution procedure -


 * 1) Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
 * 2) To make a 25 &mu;M stock, add Y&mu;L of sterile H2O to X nmoles of dry primer stock. (See equations below).
 * 3) Allow to sit for 2 mins, then vortex for 15 secs.

Short version
$$Y\ \mu L\ =\ 40\ *\ X\ nmoles\ primer$$

The number of nmoles of material in the tube (X) should be listed on the pages accompanying your primer order.

Long version
$$Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer$$