Rosenzweig:Th1 Cell Culture

Overview
This is a protocol for the culture of Th1 cells isolated from mouse spleens and lymph nodes.

Materials

 * 6.5 Thy1.1+/+ mice
 * CTLM
 * RPMI + 10% FBS
 * 10 ng/ml murine IFNgamma
 * 25 units/ml murine IL-2
 * 5 ng/ml murine IL-12
 * 10 &mu;g/ml HA MHC class II peptide
 * Scissors and forceps