User:Karmella Haynes/Notebook/miR Trigger/2011/06/11

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06/11/11

 * &#x2713; YFP/miR-luc time course: Plate cells and start dox induction

YFP/miR-luc time course > Plate JDS33-47 cells at ~1x105 in 3x 12-well plates; use U2OS plain medium > Set up three wells per time point for triplicate trials > +Dox (1μg/mL) time points:
 * 1) Sat, 6/11/11, 12 pm (55 hrs.)
 * 2) Sat, 6/11/11, 7 pm (48 hrs.)
 * 3) Sun, 6/12/11, 7 am (36 hrs.)
 * 4) Sun, 6/12/11, 7 pm (24 hrs.)
 * 5) Mon, 6/13/11, 7 am (12 hrs.) (9:30 am)
 * 6) Mon, 6/13/11, 10 am (9 hrs.)
 * 7) Mon, 6/13/11, 1 pm (6 hrs.)
 * 8) Mon, 6/13/11, 4 pm (3 hrs.)
 * 9) no dox

--> Harvest cells at 7 pm for Flow Cytometry


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