China workshop/Participants

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PKU Team
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Introduction
As the school motto “Self-discipline and Social Commitment” reflects, Tsinghua University iGEM 2007 team is focusing on endeavor and creativity as well as teamwork and responsibility in the process of cooperating with the others to accomplish a goal. The team consists of two supervisors, three advisors, eight members and the desire to explore the world of synthetic biology.

Supervisors
Prof. Chen Guoqiang Dr. Xu Feng

Advisors
Liu Zhe: PhD student in Laboratory of Structural Biology, Tsinghua University Shi Zhenyu: Senior undergraduate student majoring in fundamental science (Chemistry and Biology) Song Xindong: PhD student in Medical Systems Biology Research Center

Team Members
Chen Chen: Senior undergraduate majoring in Biology Dong Peng: PhD student in Medical Systems Biology Research Center Jiang Ming: Junior undergraduate majoring in Electronic Engineering Liu Yexing: Junior undergraduate majoring in Biology Li Keyu: Junior undergraduate majoring in Biology Yu Zhou: unior undergraduate majoring in Biology Zhao Xinyu: enior undergraduate majoring in Biology Zhou Lijun: Junior undergraduate majoring in Biology

Lab
Prof. Chen Guoqiang kindly provides us working space and reagents for experiments in his lab of Microbiology, Tsinghua University

Preliminary Proposals
How much can we disturb or control the communication between microorganisms in tremendous amount? Perhaps a chain carrying signals that we want to bruit is possible to be constructed in the society of E. coli. Then we can gain a closer insight into those tiny lives. According to former work in the realm of Oscillator, we are inspired to design special gene sequence that can endow E. coli periodical reaction in certain forms to certain signals, which can be derived from other cells or be opposed artificially.
 * E. coli Communicating Chain Construction
 * E. coli Oscillator

Contact Us
The following is the contact of the Tsinghua University iGEM 2007 team. Please feel free to contact us, we would be more than happy to communicate with you. Your advice and suggestions are welcome and would be highly appreciated. Address:

Tsinghua University IGEM Team C/O Liu Yexing, Undergraduate Student in Biology, Tsinghua University, Department of Biological Science and Technology, Haidian District, Beijing, 100084, China

Email:yexing.liu@gmail.com

Tel:+86-010-515-34104

Mobile:+86-138-1177-1678

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Team one

 * Team one

Introduction
We come from Tianjin University, one of the most famous universities in China. Our team is composed of fifteen creative young people with enormous passion for synthetic biology. The solid foundation of professional knowledge provides us firm confidence, and the enthusiastic research spirit gives us the courage to challenge the mystery of science. We believe that everything can be possible with our endeavor and wisdom.

Members

 * Graduates

Binbin Han        PHD of biochemical engineering

Shuobo Shi        PHD of biochemical engineering

Hui Wang          PHD of biochemical engineering

Tong Hao          Master of biochemical engineering

Zongbao Zheng     Master of biochemical engineering

Yanfeng Xue       Master of biochemical engineering

Hao Ma           Master of biochemical engineering

Jie Zhang          Master of Zymolysis engineering


 * Undergraduates

Xiaowen Hou       major in biological engineering

Liwei Chen         major in biological engineering

Hong Zhang        major in biological engineering

Liujie Huo          major in biological engineering

Bo Wang           major in biological engineering

Yifan Yuan         major in biological engineering

Shunhua Yang       major in biological engineering

Prof. X.M. Zhao
 * Faculty Advisors

Prof. Q.Z. Wang

Lab and funding
Prof. X.M. Zhao is kind enough to provide ‘wet’ experiments environment and ‘dry’ experiment equipment for us.

Project 1
ene circuit The aim of our project is to simulate electrocircuit in series and parallel connection by gene elements. Series circuit and parallel circuit are the simplest circuits in physics. There are three eletments in it: switch, power and resistance. What we should do is to construct three kinds of bacterials functioning as the three elements in electrocircuit, seperately: one for power, the other two for resistances(R1 and R2). Light is used as a switch. The gene circuit is connected when the bacterials are supposed to light so that the flurescence proteins with different colors in the three kind of bacterials are all expressed, while the circuit is cut when we turn the light off. In series circuit, If we cut any of the resistances or power, it will result in the disconnection of the whole circuit, while in parallel circuit, the lack of one resistance will not influence the other one. Three different kinds of HSL are used as the cell to cell signals.

Project 2
Bacterial neon light In this project ,we use gene circle to create a bacterial neon light with three different colors. the first repressor protein, LacI from E. coli, inhibits the transcription of the second repressor gene, tetR from the tetracycline-resistance transposon Tn10, whose protein product in turn inhibits the expression of a third gene, cI from λ phage. Finally, CI inhibits lacI expression, completing the cycle. The three repressors are connected with red, green, and yellow luc genes (a kind of luminescent gene) seperately. With the oscillation of the gene circle, the three colors of light will show up circularly, just as a neon light.

Contact
The following is the contact of the iGEM TJU 2007 team. Your advices and suggestions are welcome and highly appreciated.

Address:Room 310, Chemical Engineering Department, Tianjin University. Tianjin .P.R.China (300072)

Email:haotong2001@sina.com

Tel:+86-022-27892075

Team three

 * team three

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Introduction
We come from USTC, one of the most famous universities in China. We are the combination of the exact tradition of USTC and the exact vitality of the young. Rigorous daily schoolwork has provided us with a solid foundation of basic knowledge, and an innovative spirit enables us to stand on the frontier of the modern science. Our belief is'Never to say failur'.

Member

 * Graduates

Zhan Jian :  PHD candidate in Key Laboratory of Structural Biology

Ding Bo:     PHD candidate in Key Laboratory of Structural Biology

Ma Rui:      PHD student in Laboratory of Nonlinear Chemistry

Ma Xiaoyu:   PHD student in National Laboratory for Physical Sciences at Microscale


 * Undergraduates

Su Xiaofeng: major in molecular and cellular Biology

Liu Ziqing:  major in molecular and cellular Biology


 * Faculty Advisors

Prof. H.Y. Liu

Prof. J.R. Wu

Prof. Z.H. Hou

Lab and funding
Prof. J.R. Wu is kind enough to provide ‘wet’ experiments environment.

Our team is sponsored by the National Science Funding and the CYL committee of USTC.

Proposal
Despite the exciting advancement in recent years, phage contamination is still one of the headaches that nowadays the food and fermentation industries mainly confront. Some strains with genotype like TonA is resistant to T1-like bacteriophages, but the productive performance of these strains is low because of the slow growth rates. Therefore, the producer is facing a dilemma: how can we deal with the strains lacking phage resistance, but with high productivity, and strains with certain kind of phage resistance but the productivity of which is rather low.

To help those unfortunate guys, we aim at developing an inexpensive system to offer the industries a new kind of strain that can grow fast and without phage infection.

There are two different strategies for the active defense:

One is the ‘Signaler Strategy’, that is, let a cell infected with bacteriophages send a signal telling other cells that the enemy is coming. In this way the others will switch on the expression of TonA, making themselves resistant to the enemy.

There are two key points for this strategy. Firstly, what can be used as such a signal? It should be rare in the environment in most occasions, and its number ought to increase fast after the infection. Secondly, how to switch on the expression of TonA? The response should be as quick as possible, or the enemy will invade in without any resistance!

The other strategy is the ‘Sacrifice Strategy’, that is, when the phage begins to reproduce itself, the apoptosis system of the host will be switched on. The host commits suicide to avoid the propagation of the phages, and without influencing the others.

Also, there is a key point for this strategy, that is, how to make the reaction as quick as possible .And another one is how to keep the apoptosis off under normal conditions.

Both of the strategies might be applied to reinforce the self-defense ability of cells.

Contact
The following is the contact of the iGEM USTC 2007 team. Your advice and suggestions are welcome and highly appreciated.

Address:Room 456, Life Sciences Building, University of Science and Technology of China Hefei, Anhui, P.R.China (230027)

Email:zhanjian@ustc.edu

Tel:+86-551-3603754-1

Mobile:+86-13856942160

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