Kubke Lab:Research/CND/Records/RC001

=Embryo details=

Species: Gallus gallus domesticus Embryo Name: RC001 Embryo stage: ST22 Confirmed by Fabiana. (see Notebook Entry) Staging description: Staged as a group exercise by Fabiana, Malisha and Reuben using the Hamburger and Hamilton (1951) staging system. Fixation: Cryoprotection: Cryoprotected in 30% Sucrose in 0.1M PBS Material label and storage: Labeled as RC-001 and stored in sucrose in a vial prior to being cut. The slides produced by RC001 are in a slide folder in the lab labeled RC001.

=Experiment details=

Objective: To use the embryo in a staging demonstration (see Notebook Entry), cryoprotection practice and cryostat sectioning practice. Procedure: 23/11/2011 see Notebook entry 24/11/2010: Histology Training with Satya see Notebook entry
 * Embryo taken from 'stationery draw' where it was stored in PFA.
 * As a group we staged the embryo and Fabiana labeled it as ST22, RC001.
 * It was put into a vial filled with 30% sucrose, 0.1M PBS.
 * The embryo was placed flat on a plastic mould and OCT was then added.
 * The embryo was oriented 90° to the side of the block so that coronal sections could be obtained.
 * The protruding OCT was cut from the sides of the block.
 * The block was mounted onto the chuck at 90° to the chuck.
 * A mark was made on the block corresponding to the line drawn on the metallic chuck holder, so that the block could be re-aligned if it had to be moved from the metallic chuck holder.
 * The embryo was then sectioned in the cryostat.

Comments: Tissue from this embryo will not be used for histological analysis. RC001 was used for practice purposes only, it was not stained or coverslipped. The settings of the cryostat and the cutting techniques were altered to learn the effects the settings had on the quality and efficiency of the sections produced. No further work will be done with RC001.

=Results= =Images= =Summary=