User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/04

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Drosophila melanogaster Genetics
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Spermatogenesis

 * 9 Day Heat Shock
 * Dissect Control and Experimental
 * Fix 20' in 4% formaldehyde @ RT
 * Wash 4 x 3' in PBS-T @ RT
 * GFP-Myb, RFP H2Av
 * Wash 3 x 15' in PBST
 * Stain 2 hrs @ RT with secondary
 * anti-PCNA mouse (use anti-mouse 633)
 * anti-BAM mouse (use anti-mouse 633)


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