Wikiomics:Site Directed Mutagenesis

This page is beta. Please feel free to update it.

The idea here is to create a flow of work to simulate the point mutation of a single amino acid in a protein.


 * 1) First step is to choose your target. (This is up to you.)
 * 2) Finding the nucleic sequence (ENTREZ)
 * 3) What type of mutation will be introduced ? (substitution / deletion / addition)
 * 4) Can we find the wanted mutation in other related proteins ? (Blast)
 * 5) How conserved is the protein we wish to mutate ?
 * 6) Does the w.t. protein have a known 3D structure ? (Check PDB)
 * 7) If not, check out STRUCTURE PREDICTION FLOWCHART
 * 8) What are the known domains inside our protein ?
 * 9) see Wikiomics:Searching for 3D functional sites in a protein structure
 * 10) Where is the Active Site ? (SARIG)
 * 11) What is the location of the mutation? (surface / inside the protein)
 * 12) What impact does the mutation have on the protein? (no backbone changes yet)
 * 13) If the protein is a multiple subunit protein - what are the subunit interaction residues ? (CSU)