IGEM:MIT/2006/Notebook/2006-7-27

To Do

 * 1) Miniprep overnight cultures
 * 2) Ligations + Digestions
 * 3) Pour more LB Amp plates
 * 4) Retransform pchBA and osmY promoter + pSB1A3-1
 * 5) Transform ligations

Digestions

 * 1) E0840 w/ EX
 * 2) osmY w/ S
 * 3) ATF1-1148 w/ ES
 * 4) B0030 w/ SP
 * 5) B0032 w/ SP

Ligations

 * 1) B0030(2)SP + 45001.B0015CXP
 * 2) B0030(2)SP + ATF1-1148XP
 * 3) osmYES + E0840EX
 * 4) ATF1-1148ES + B0015EX

Transformations (using new cells from Tom)

 * 1) B0030(2)SP + 45001.B0015CXP
 * 2) B0030(2)SP + ATF1-1148XP
 * 3) osmYES + E0840EX
 * 4) ATF1-1148ES + B0015EX
 * 5) pchBA w/ pchA silent mutation (transformation failed yesterday)
 * 6) osmY + pSB1A3 backbone (transformation failed yesterday)
 * 7) pUC19

Desired Completed Constructs

 * 1) RBS-SAMT-Term
 * 2) RBS-ATF1
 * 3) SPP-RBS-GFP-Term
 * 4) ATF1-Term

Listed Current Biobricks
Can be found at in the index

Made liquid cultures of transformants

 * R0040+B0030 [A, B, C, and D] (in LBAmp)
 * R0040+B0032 (in LBAmp)