User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/06/15

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ABSTRACT

 * Beginning of the preparation of two solutions (TE-10:1 and Solution I) needed for plasmid isolation.


 * My goal of today was the preparation of two solutions for plasmid isolation, because our reserves had been finished. For that I follow the protocols that Miguel gave us. For the preparation of TE-10:1 I need the following proportions of reagents:

And for the preparation of Solution I, the proportions are:


 * So the first step was to prepare the stock solutions of EDTA 0.5M pH 8, EDTA 0.25M pH 8 and glucose 0.5M. The solution of Tris 1M pH 8 was already made. So for prepare the stock I follow the next indications:

0.5M EDTA pH 8 Adjust the pH with NaOH, filter and sterilize.

0.25M EDTA pH 8

Glucose 0.5M


 * I prepare this three solutions and I left in the autoclave the solutions of EDTA. Tomorrow I´m going to finish solutions TE-10:1 and Solution I.