Talk:Synthetic Biology:Vectors

Vector design review meeting
We held a meeting to review the proposed design for some new BioBricks standard vectors.

Meeting notes

 * Are there any other features that we want in these vectors?
 * All BioBricks compatible restriction sites should be removed from the vectors once the resistance marker and replication origin have been inserted.
 * Only include a single antibiotic resistance marker (rather than two).
 * Include a unique restriction site in the backbone for situations where the insert is equal in size to the backbone (such that they can be distinguished).
 * Since all components will be synthesized, remove unnecessary restriction sites. Also do codon optimization?  See Codon pairs.
 * Should the flanking terminators be inside or outside the verification primer binding sites?
 * Inside.
 * Plasmid barcode
 * Use a 3-4 bp tag that can be read during sequencing. Also place this tag immediately 3' to VF2 and VR so that vector specific primers can be used in single colony PCR?
 * How can we "verify" the design?
 * Caitlin, Barry and TK have volunteered to inspect the design.
 * How should the vectors be fabricated?
 * Direct synthesis of useful vector components, scaffold and intact vectors (budget of $20,000). Prepare a flow chart of synthesis and cloning steps so that we can obtain useful intermediates and test whether they work or not.

Relevant pages

 * BioBricks parts
 * Single copy plasmid
 * Synthetic Biology:Vectors/Barcode
 * Codon pairs