Initial Testing for DNA constructs for CELL BY DATE In Vitro

Aims:
 * To determine which constructs for cell by date expresses in vitro
 * To test the pTet and pcI constructs in commercial S30 E.coli cell extract and home made S30 extract
 * To test the pT7 construct in commercial S30 T7 cell extract and home made S30 extract.
 * To investigate optimum volume of home made S30 extract
 * To investigate the differences between home made and commercially bought S30 extract. This is in terms of rates of expression, length of expression and total output.