IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-24

Precipitation tests

 * Made 10 mL solution with Dr. Shih's protocol.
 * Tested (which DNA?) with the PEG precipitation protocol.

Streptavidin binding assay

 * For the future...
 * Double asterisks indicate need for purified nanostructures
 * Total volume for each reaction: 13

Working stocks
Final concentration of each oligo in working stocks is 250 nM.

Folding reaction
Reagents (each expt in respective 0.2 mL PCR tube) Annealing protocol
 * start at 80
 * 60 cycles: wait 2 minutes, decrease 1
 * hold at 4