Kubke Lab:/Notebook/Cranial nerve development/2011/02/04

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Cranial Nerve Development
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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=General Entries= =Personal Entries=
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Malisha
Plan for today:
 * Complete entering embryo records including some results, type of stain and staining procedure--Malisha Hettiarachchi 21:53, 3 February 2011 (EST).

MH006- Must go back and stain these sections. MH003

- Nice animation showing 3D embryo development
 * Complete the required headings in the neuroanatomy document.
 * Follow up embryo atlas

-Trying to figure out a way to collate the information on google docs so both me and Reuben are able to contribute to the document more collaboratively --Malisha Hettiarachchi 19:55, 3 February 2011 (EST).

Histology
Using the following resource previously mentioned here a set of criteria will be created to assess the quality of the [|Nissl stained] sections:
 * A nice video showing coronal sections of mouse brain Nissl stained.
 * Nissl stain of mice cerebellum
 * Nissl stain of telencephalon description found here
 * "Mammalia - Felis - Cat spinal cord smear Nissl "
 * 1) Do the cell bodies appear violet to dark blue?
 * 2) Under higher magnification are subcellular organelles visible?
 * 3) Is there evidence of cell loss?

Questions to answer: The neurons should be stained dark violet against a lighter background colour. The following image illustrates this :
 * What would the neurons look like in the Nissl stained sections?

Reuben

 * 10:30am-12:30pm Cresyl violet stain of MH007. The Slides are drying on the benchtop.
 * 1:45pm Working online with Malisha.--Reuben Cutfield 19:53, 3 February 2011 (EST)

Fabiana

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