IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/16

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Team Allergy
More arabadopsis DNA arrived today. We are going to obtain more hpRNA parts and

GFP didn't show up (first 2 lanes). The plants for the extraction weren't the GFP variety so this is expected.
 * Did a second PCR/Purification of our allergen panel from Julia's arabidopsis genomic DNA. It worked!


 * gel purifying PCR products
 * Concentrations: LTPS: LTPA: GERS: GERA: BETS: BETA: BET2S: BET2A: PME: PAL:


 * Digested Xba1 Pst1
 * Sent BetS, PDK2, and GerS (3) for sequencing
 * Miniprep amiRNA

Nanodrop of Miniprepped amiRNA Concentrations

Diagnostic Gel: Ladder, Bet1-6, LTP1-LTP4, Ladder; Ladder, LTP6, LTP.5 (1-6), Ladder



PCR of Arabidopsis Genomic DNA, attempt 2
PCR failure - no product.

PCR using the newly extracted DNA was unsuccessful.

The degradation signal from the Austrians was successfully inoculated and colonies were found on the agar plates. Individual colonies from the plates were inoculated into tubes of 5 ml LB+Amp and placed in the shaking incubator overnight. (37°C)

Making Glyerol Stocks
Combined .5mL 80% glycerol with .5mL overnight cell culture of: Barnase 3 (number changed to Barnase 1 from miniprep onward), LacIN1, and Gal2.

Team Flavor

 * Did minipreps of the Mira/Brazz + Strep constructs. All constructs had failed to ligate.


 * Ran PCR of Valencene gene from extracted genomic DNA. Followed with PCR purification
 * Re-digested STOP+V0120 BioBrick, gel purified. Gel Purification failed, no DNA was extracted.


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