User:Tara K. Luckau/Notebook/Team ConGen/2010/10/21

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 * style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]] Tara's Lab Notebook
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 * style="background-color: #9DB68C" align="center"|  |Main project page


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Make 1x TAE

 * 20mL 50x TAE + 980 mL NanoPure H2O

Cast Gel

 * 100mL 1x TAE + 1.5g agarose
 * large stirbar, hotplate set at 150 for about 30 min
 * let cool about 20 min
 * not enough volume! FAIL!

Next Try

 * 270mL TAE + 4.05g agarose
 * (for future reference, total volume ≈ volume of gel box in cm3, ie 27cm long x 20 cm wide x 0.5 cm thick gel = 270mL of TAE)


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