IGEM:IMPERIAL/2006/LabCalendar/2006-9-25

 TO DO: *Miniprep of J37024 and J37031 (from the o/n ligations) *Ligations and Electroporations: J37022 (AiiA test construct) J37025 (to be used to construct the final poly/monocistronic predator parts) J37018 (to be used to construct the final monocistronic predator part) J37020 (the polycistronic prey test construct)

Minipreps

 * J37024 colonies 2 and 3 successful (colony 1 unsuccessful)
 * J37031 all three colonies successful

Ligations and Electroporations

 * Ligation set up today.
 * Dr. Mann said he'd transform if he was still around later today or else he'll do it tomorrow...things are looking up, we may get our test construct yet.

Cre-Lox

 * Using new blunt-end kit, the ligation of the Cre-Lox part into a blunt-ended plasmid
 * Left to ligate overnight