User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/19

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04/19/10

 * &#x2713; Sequencing order: KAH154-159/MV2; KAH96, 142, 146, 147/V0201

Sequencing order (Genewiz, standard, 4/20/10) --> Mix-up: Sequence reactions were actually set up (by me) in this order: 154, 155, 156, 158, 159, 157
 * 1) KAH154/MV2 DDB122: good
 * 2) KAH154/MV2 DDB123: good
 * 3) KAH155/MV2 DDB122: good
 * 4) KAH155/MV2 DDB123: good
 * 5) KAH156/MV2 DDB122: fshPcCD! (should be fly)
 * 6) KAH156/MV2 DDB123: fshPcCD! (should be fly)
 * 7) KAH157/MV2 (actually 158) DDB122: good
 * 8) KAH157/MV2 (actually 158) DDB123: good
 * 9) KAH158/MV2 (actually 159) DDB122: good
 * 10) KAH158/MV2 (actually 159) DDB123: good
 * 11) KAH159/MV2 (actually 157) DDB122: flyPcCD! (should be fsh)
 * 12) KAH159/MV2 (actually 157) DDB123: flyPcCD! (should be fsh)
 * 13) KAH96/MV2 3t3: good
 * 14) KAH96/MV2 BGHR: good
 * 15) KAH142/MV2 3t3: good
 * 16) KAH142/MV2 BGHR: good
 * 17) KAH146/MV2 3t3: looks like 147
 * 18) KAH146/MV2 BGHR: good
 * 19) KAH147/MV2 3t3: looks like 146
 * 20) KAH147/MV2 BGHR: good

--> Tomorrow: check KAH156, 157/MV2 with AvaI digest for correct i.d.; also XbaI digests for KAH94, 142, 146, 147/V0201


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