SBB09 Construct18

Wobble Odvs002F/Odvs002R to make M10023 (Bdvs002)  (67 bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5                      (EcoRI/BamHI, 3171+910, L) Product is pBca9495AK-M10023          {}       (3222bp)

Odvs002F   Forward construction of AG4 basic part       ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC Odvs002R   Reverse construction of AG4 basic part       ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG