Quint Lab:colony pcr

{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"
 * -valign="top"
 * width=300px style="padding: 5px; background-color: #ffffff; border: 2px solid #F8B603;" |

colony pcr

 * 1) pick half of an isolated colony with a toothpick of pipet tip and inoculate 25 µl TE; store the plate parafilmed at 4ºC.
 * 2) boil for 5 min.
 * 3) spin down at 13k for 1 min.
 * 4) use 2 µl as template for subsequent pcr.
 * 5) run out on a gel with respective control.
 * 6) pick other half of the positive clones and inoculate liquid o/n culture for miniprep.