IGEM:Peking University/2008/Notebook/Group 2/2008/05/17

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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Primer design

 * Primer 1
 * Primer name: F-Gal4-HindIII-Apal
 * Sequence(5'to3'):AAAAAGCTTGGGCCCTTTTAAGAGAGGACAGAGAAGCAAGCCTC
 * Tm:64.1°C
 * Primer 2
 * Primer name: R-Gal4-EcoRI-Xhol
 * Sequence(5'to3'):GGGGAATTCCTCGAGTGCGGGGTTTTTCAGTATCTACGATTCATT
 * Tm:64.0°C

PCR

 * conditions


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