SBB09 Construct32

Biobricking of Intimin EaeA

PCR OWRL-023-F/OWRL-024-R on {} 	(1887bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 				(EcoRI/BamHI, 910+3039,L) Product is pBca9495CA-M10032 --- OWRL-023-F	Forward biobricking of Intimin EaeA GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG OWRL-024-R	Reverse biobricking of Intimin EaeA GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC

JCA: Reverse oligo can be reused. See revision:

Biobricking of Intimin EaeA

PCR OWRL-023-F/Bhs003R	 on {} 	(1888 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 				(EcoRI/BamHI, 910+3039,L) Product is pBca9495CA-M10032 {} --- OWRL-023-F	Forward biobricking of Intimin EaeA GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG Bhs003R	Reverse BamHI for a~eaeA_Display> GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC