Template:SBB-EcoBamXfers

Eco/Bam Transfer
Set up digest: For BBb transfers: 7.5uL ddH2O 1 uL NEB2+ATP Buffer 0.5uL EcoRI 0.5uL BamHI 0.5uL miniprepped plasmid
 * Set up the digest:
 * run temperature program on thermocycler of 1hr at 37 and then 20 min at 80
 * Add 0.5uL of predigest vector and 0.5uL of T4 DNA ligase
 * Incubate 30 min. at room temp, transform, rescue, plate.