User:Niepelm/Notebook/ICBP45/2010/09/22

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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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HCC-1419 density

 * at 5pm Lili treated
 * by mistake fixed Serum plate shortly after treatment!
 * failed to take microscope images

HCC-1187 CNI-96well

 * at 10am treat 96 well dishes
 * some problems in serum starvation
 * some columns have too much media (most likely serum media not taken off completely)
 * treat anyway
 * maybe retreat since 15cm dishes of this line were dead anyway


 * primary Ab added at 6pm
 * incubate o/n

HCC-1806 SS-15cm

 * at 2pm treat 15cm dishes with Lili
 * took some extra time to show everything
 * overall samples should be good
 * no problem

HCC-1806 CNI-96well

 * at 3:30pm treat 96 well dishes
 * a little late (by two hours maybe)
 * overall starvation looks good
 * so far no problems
 * keep ImageWorx dishes o/n in 200yl PBST


 * GF1 30min wells (3,4,9,10) treated with plate upside down. Readjust after scanning.

HCC-1569 density

 * serum starved

HCC-1954 density

 * serum starved

HCC70 SS-15cm

 * at 1pm plated 10x 15cm dishes

MCF7 PNNL-15cm

 * at 4pm plated 8x15cm for collaborators

MCF7 RPLA-96well

 * at 4pm plated 14x96 well for RPLA


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