User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/06/02

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 * style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] Maize Endophyte Biofertilizers
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 * style="background-color: #F2F2F2" align="center"|  |Main project page


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The Daily Grind

 * Transformed a 0.25 ul per sample pool of 16S PCR from all the seeds into TOPO
 * Photographed and weighed nodal potatoes
 * Screened ACC deaminase clones for growth in ACC amended LGI but none had grown overnight - transfered previously selected hits to antibiotic selected media and grew overnight along with alsD clones for glycerol stock and plasmid extraction. Also doing PCR screen for the transgene.
 * Finsihed assigning species names to endophytes in 96 well plate formats


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