<owwmenu font="arial, helvetica, sans-serif" bold="1"
color="black" bgcolor="white" hovercolor="black"
bghovercolor="gray" topfontsize="10" fontSize="8" pagewidth="930"
image="Bishop.jpg">
Home=Pelling
Protocols=Pelling:Protocols
Lab Supplies=Pelling:Lab Supplies
Lab Safety and MSDS's=Pelling:Lab Safety
</owwmenu>
Fetal Bovine Serum (FBS)
- FBS comes frozen in a 500mL bottle and is stored in the -80°C Freezer.
- Thaw in a water bath or overnight in the fridge.
- In a sterile hood make 10x50mL aliquots of the FBS in 50mL falcon tubes.
- IMPORTANT: If more FBS is required for Wash Buffer then make 5x20mL aliquots in the 'flip-cap' tubes and 8x50mL aliquots in 50mL falcon tubes.
- Store in the -20°C freezer.
Penicillin/Streptomycin (P/S)
- Thaw a 100mL bottle of P/S in the water bath.
- In a sterile hood make 20x5mL aliquots in the 5mL screw cap tubes.
- Store in the -20°C freezer.
Trypsin-EDTA (TE)
- TE comes frozen in a 500mL bottle and is stored in the -20°C Freezer.
- Thaw in a water bath or overnight in the fridge.
- In a sterile hood make 11x45mL aliquots in 50mL falcon tubes (NOTE the 45mL aliquot size)
- Store in the -20°C freezer.
|
